• Meanwhile, marker genes transcription levels were not different in both short day and long day growth condition. (helsinki.fi)
  • Genetic fine-mapping and CRISPRi screens identify functional variants and their target genes associated with Alzheimer's disease in microglia. (nature.com)
  • Regulation of transcription of genes required for fatty acid transport and unsaturated fatty acid biosynthesis in Escherichia coli by FadR. (microbiologyresearch.org)
  • Identification and in silico characterization of structural and functional impacts of genetic variants in milk protein genes in the Zebu breeds Guzerat and Gyr. (fiocruz.br)
  • Whole genome sequencing of bovine breeds has allowed identification of genetic variants in milk protein genes. (fiocruz.br)
  • Although over expressing any of these six single-repeat R3 transcription factors in Arabidopsis causes a glabrous phenotype, single mutants of these genes have different phenotypes. (biomedcentral.com)
  • The processes of animal cell growth, differentiation and infection by viruses result from the differential expression of specific genes, controlled primarily at the level of transcription. (rockefeller.edu)
  • To this end, our specific objectives are to determine the nature and mechanism of action of both the general transcription initiation factors that are commonly used by all genes and the gene- and cell type-specific factors that directly regulate target genes in response to various growth, developmental and viral stimuli. (rockefeller.edu)
  • The transcription of protein-coding genes involves RNA polymerase II and a number of common factors (TFIIA, -B, -D, -E, -F and -H) that form functional preinitiation complexes (on promoters) via an ordered assembly pathway. (rockefeller.edu)
  • Similarly, the transcription of small structural RNA genes (5S RNA, tRNA) by RNA polymerase III involves the assembly of common factors (TFIIIC and TFIIIB) into highly stable complexes via an ordered pathway. (rockefeller.edu)
  • Conservation of genes and processes controlled by the quorum response in bacteria: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis Natalia Comella and Alan D. Grossman* Department of Biology, Building 68-530, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. (igem.org)
  • For identification and characterization of genes involved in the expressional control of antioxidant enzymes several Arabidopsis mutants with low activation of the 2-Cys peroxiredoxin-A (2CPA) promoter were isolated from a chemically mutagenized reporter gene line, which expresses luciferase under control of the redox-regulated 2CPA promoter. (fu-berlin.de)
  • These findings suggest a scenario where collisions between DNA replication and transcription are frequent, leading to increased genetic variability, as seen by the increase SNP levels at chromosome subtelomeres and in DGF-1 genes containing putative origins. (biomedcentral.com)
  • We analyzed the genetic profile of Mhp field samples using 16 genes that encode proteins involved in the mechanisms of bacterial pathogenesis and/or the immune responses of the host. (biomedcentral.com)
  • This work demonstrated the genetic diversity of several Mhp field strains based on 16 selected genes related to virulence and/or immune response in Brazil. (biomedcentral.com)
  • Based on our results, we suspect that Mhp genetic variability may be associated with the frequency of genes among the field strains and we have demonstrated that some Mhp field samples could not have many important genes described in the literature. (biomedcentral.com)
  • In our work, we suggest the use of a PCR assay associated with bioinformatic tools for the analysis of genetic differences based on several Mhp genes. (biomedcentral.com)
  • The ALX3 protein is a transcription factor, which means that it attaches (binds) to DNA and controls the activity of certain genes. (medlineplus.gov)
  • Escherichia coli unsaturated fatty acid synthesis: complex transcription of the fabA gene and in vivo identification of the essential reaction catalyzed by FabB. (microbiologyresearch.org)
  • Identification and genetic characterization of viruses of sweet potato. (agric.za)
  • We focus on the identification and molecular characterization of human genetic variation that alters the gene regulatory capacity of transcription factors and contributes to diseases with identified genetic component like type 2 diabetes or Alzheimer's disease. (uef.fi)
  • The identification and mechanistic characterization of new, truly causal human genetic variants in complex diseases offer tools for improved disease prediction, subtyping and personalized medicine solutions. (uef.fi)
  • Identification of Functional Genetic Variations Underlying Drought Tolerance in Maize Using SNP Markers [J]. J Integr Plant Biol. (jipb.net)
  • CTCs are present in the bloodstream at a very low concentration, thus their detection and characterization require highly sensitive and specific methods, which consist of a combination of enrichment (isolation) and detection (identification) strategies ( Table 1 ) and both steps are essential components of the identification process ( 19 ). (amegroups.org)
  • Identification and Characterization of Novel Small RNAs in Rickettsia prowazekii. (utmb.edu)
  • Among the many achievements ofo his team are the identification and characterization of several "new" viruses, including the human metapneumovirus (hMPV), human coronavirus NL63, the SARS coronavirus (SARS-CoV), the MERS coronavirus (MERS-CoV), and a new influenza A virus subtype (H16). (ed.ac.uk)
  • We conducted cell-type-specific genetic fine mapping of AD variants and performed extensive functional characterization to unravel the causal variants that contribute to transcriptional regulation and AD-related phenotypes in microglia. (nature.com)
  • 2018). Functional characterization of TBR1 variants in neurodevelopmental disorder. (mpi.nl)
  • The role of these candidates is tested using gene knock-out plants and functional characterization of confirmed candidates is performed using a combination of physiological, biochemical and genetic approaches. (mpg.de)
  • We produce proteins of interest recombinantly in E. coli and use mainly fluorescence, far-UV circular dichroism (CD) and Fourier-transform infrared (FTIR) spectroscopy for structural and functional characterization. (mpg.de)
  • On the other hand, although the transcription of TCL2 , like TCL1, is not controlled by the activator complex formed by GL1 and GL3, and TCL2 and TCL1 proteins are more than 80% identical at the amino acid level, the expression of TCL2 under the control of TCL1 promoter only partially recovered the mutant phenotype of tcl1 , implying that TCL2 and TCL1 are not fully functional equivalent. (biomedcentral.com)
  • Feline infectious peritonitis: insights into feline coronavirus pathobiogenesis and epidemiology based on genetic analysis of the viral 3c gene. (cdc.gov)
  • Transcription Factors, Gene Regulatory Networks, and Agronomic Traits. (routledge.com)
  • Based on these results, we formed a hypothesis that gene redundancy could influence genetic characterization of WRKY75. (helsinki.fi)
  • Our methods test the impact of gene variants on the properties and functions of encoded proteins through the genetic manipulation of human cells that we can grow in the laboratory. (mpi.nl)
  • Escherichia coli FadR positively regulates transcription of the fabB fatty acid biosynthetic gene. (microbiologyresearch.org)
  • Gene expression studies provide us with a selection of candidate transcription factors and other regulatory proteins that are related to such memory functions. (mpg.de)
  • Our gene fusion characterization workflow revealed two potential novel fusions specific for PCa. (biomedcentral.com)
  • Since the ubiquitous general transcription factors represent the ultimate targets of the various gene-specific factors, their characterization is essential. (rockefeller.edu)
  • Here, we present data revealing the genetic network associated with ACVRL1 (the gene encoding for ALK1) expression in human cancer tissues. (lu.se)
  • Genetic analysis indicated that the brittle of fp2 was controlled by a recessive gene. (jipb.net)
  • In this study, we report the cloning and characterization of the transcriptional regulation of the mouse UGT1A1 gene. (aspetjournals.org)
  • Translocation carcinoma is a renal cell carcinoma (RCC) subtype that harbors a translocation involving a member of the microphthalmia transcription factor gene family. (medscape.com)
  • The responsible gene has been mapped to band 12q24.1, which encodes the human transcription factor TBX5. (medscape.com)
  • Genetic studies have associated thousands of non-coding variants with Alzheimer's disease (AD), yet the functions of these variants remain elusive. (nature.com)
  • As such, M13 was further engineered to incorporate variants of the zinc finger, DNA-binding domain of transcription factor Zif268 as minor coat fusions. (mit.edu)
  • WRKY transcription factors is known as one of the largest transcription factor family in plants which regulates a variety of plants processes. (helsinki.fi)
  • Previously we identified a novel single-repeat MYB transcription factor, TRICHOMELESS1 (TCL1), which negatively regulates trichome formation on the inflorescence stems and pedicels by directly suppressing the expression of GL1 . (biomedcentral.com)
  • The ComX-ComP-ComA signalling pathway is a major quorum response pathway in B. Subtilis and regulates the development of genetic competence (7). (igem.org)
  • MYB56 Encoding a R2R3 MYB Transcription Factor Regulates Seed Size in Arabidopsis thaliana [J]. J Integr Plant Biol. (jipb.net)
  • At the biochemical leve l, CBDA raises anandamide levels, activates a serotonin receptor, acts as an antioxidant , regulates genetic transcription , and inhibits the COX-1 pathway (a pathway also used by aspirin). (cvresearch.info)
  • The implementation of molecular and genomic characterization of CTCs could contribute to improve the treatment selection and thus, to move toward more personalized treatments. (amegroups.org)
  • Regulation of transcription factors via natural decoys in genomic DNA. (utmb.edu)
  • In addition, we use genetic approaches such as RNA interference, overexpression or GFP tagging to study the function and localization of the proteins in planta . (mpg.de)
  • We focus our attention on three families of transcription factors that are known to have roles in vertebrate chondrogenesis: Sox and the Ant- and Prd- class families of the homeodomain proteins. (europa.eu)
  • As a way to demonstrate the utility of cell-free TX-TL, we illustrate the characterization of two genetic networks: an RNA transcriptional cascade and a protein regulated incoherent feed-forward loop. (biorxiv.org)
  • Access to human germline cell types for study is limited, and little is known about the underlying genetic regulation of human gametogenesis. (harvard.edu)
  • These strategies involve rearrangements at the molecular level starting from transcription, regulation of mRNA processing, translation, and protein modification or its turnover. (intechopen.com)
  • Plants show stress-specific regulation of transcription that affects their transcriptome under stress conditions. (intechopen.com)
  • The early research focused on the sedative properties but now it's been more widely studied for nausea , anxiety , the lowering of inflammation and pain , anti-tumor effects via changes to genetic transcription , and in a model of Alzheimer's , working with THCA to protect the brain and rescue memory deficits. (cvresearch.info)
  • TFIIF binds to RNA polymerase II when the enzyme is already unbound to any other transcription factor, thus preventing it from contacting DNA outside the promoter. (wikipedia.org)
  • To evaluate the function of WRKY75 transcription factor in SA signalling and cell death, three lesion mimic mutants acd5, cat2, dnd1 and their corresponding wrky75 double mutant were used. (helsinki.fi)
  • The basic leucine zipper (bZIP) transcription factor represents exclusively in eukaryotes and can be related to many biological processes. (biomedcentral.com)
  • We analyzed here the role of TRICHOMELESS2 (TCL2), a previously-uncharacterized single-repeat MYB transcription factor in trichome patterning in Arabidopsis . (biomedcentral.com)
  • In Bacillus subtilis, a quorum response is mediated by a secreted 10-amino-acid modified peptide (ComX pheromone) that activates a receptor histidine kinase (ComP) that activates a response regulator transcription factor (ComA). (igem.org)
  • As a transcription factor whose expression is increased by DNA damage, p53 blocks cell division at the G1 phase of the cell cycle to allow DNA repair. (medscape.com)
  • The CDK4-cyclinD complex normally phosphorylates the retinoblastoma protein (Rb protein), leading to release of the E2F transcription factor and cell cycle progression. (medscape.com)
  • Together, these results provide evidence for a major regulatory function of this liver-enriched transcription factor in UGT1A1 activity in both rodents and human. (aspetjournals.org)
  • A cardiomelic developmental field has also been postulated to relate the genetic heterogeneity of HOS (and other similar syndromes) to a cascade of molecules, including the brachyury, sonic hedgehog, bone morphogenetic protein, retinoic acid receptor, and transforming growth factor beta families. (medscape.com)
  • Partial genetic characterization of West Nile virus strains, New York State, 2000. (cdc.gov)
  • It was also noted that samples from the same farm were genetically different from each other and samples from different regions could be genetically similar, which is evidence of the presence of different genetic profiles among the Mhp field strains that circulate in Brazilian swine herds. (biomedcentral.com)
  • Therefore, it is important to investigate the genetic diversity and variability of Mhp field strains to improve the vaccines and develop more specific and effective diagnostic methods. (biomedcentral.com)
  • The aim of this study was to investigate the genetic profile of Brazilian Mhp field strains in order to help the development of more effective vaccines against Mhp, as well as specific diagnostic tests for Mhp. (biomedcentral.com)
  • Grossman, A.D. (1995) Genetic networks controlling the initiation of sporulation and the development of genetic competence in Bacillus subtilis. (igem.org)
  • Abstract: Effective interpretation of genome function and genetic variation requires a shift from epigenetic mapping of cis-regulatory elements (CREs) to characterization of endogenous function. (harvard.edu)
  • Our broad objectives are to understand the specific regulatory events that control these processes, as well as more fundamental aspects of transcription activation and repression mechanisms. (rockefeller.edu)
  • Genetic characterization of viruses infecting sweet potato in South Africa. (agric.za)
  • Furthermore, because of the antigenic and genetic diversity of 'Norwalk-like viruses'* (NLVs) and the inability to cultivate these viruses in cell lines, developing assays to detect the full spectrum of NLVs associated with outbreaks of gastroenteritis was not possible. (cdc.gov)
  • Constant monitoring of genetic changes in the circulating influenza A(H1N1)pdm09 viruses is important for maintaining the sensitivity of molecular detection assays. (who.int)
  • Both conventional RT-PCR and real-time RT-PCR assays are rapid, sensitive methods for detecting the genetic material of influenza viruses. (who.int)
  • Genetic Diversity among Lathyrus ssp. (mdpi.com)
  • The characterization of the diversity of species living within ecosystems is of major scientific interest to understand the functioning of these ecosystems. (intechopen.com)
  • In genetic diversity studies, the most frequently used markers are microsatellites. (intechopen.com)
  • The allelic frequency data were used to estimate genetic similarities among lines, and as a result the diversity index of 0.499 was obtained. (jipb.net)
  • Techniques such as the antibody-based enzyme linked immunosorbent assay (ELISA), nucleic-acid methods such as reverse transcription polymerase chain reaction (RT-PCR) and PCR are used. (agric.za)
  • During the early 1990s, breakthroughs in cloning and sequencing of Norwalk virus and Southampton virus ( 15--18 ) led to the development of sensitive molecular assays (e.g., reverse transcription-polymerase chain reaction [RT-PCR]), nucleotide hybridization probes, and enzyme-linked immunosorbent assays (ELISA) that used baculovirus-expressed viral antigens ( 19--33 ). (cdc.gov)
  • There are two methods of reverse transcription polymerase chain reaction (RT-PCR) that have been the common methods to detect influenza infections: conventional and real-time RT-PCR. (who.int)
  • The gold standard assay for influenza diagnosis is the reverse transcription polymerase chain reaction (RT-PCR) assay. (who.int)
  • Furthermore, the high polymorphism of retrotransposable elements can represent a starting point for the development of new genetic variability markers. (sisef.it)
  • High-Throughput Approaches for Characterization and Efficient Use of Plant Genetic Resources. (routledge.com)
  • Retrotransposable elements are important and peculiar genetic components derived from ancient retrovirus insertion inside plants genome. (sisef.it)
  • Transposable elements (TEs) are ancient (retro)-virus insertions inside a host genome and are peculiar mobile genetic elements accounting for a large proportion of repetitive DNA regions ( [29] ). (sisef.it)
  • Targeted genome editing using CRISPR-Cas9 has been widely adopted as a genetic engineering tool in various biological systems. (mdpi.com)
  • Genome replication is responsible for accurate transmission of genetic information through cell division cycles. (biomedcentral.com)
  • As originally proposed in 1963, cells rely on two genetic elements to duplicate their genome: the replicator, a DNA region where replication begins (now named the replication origin), and the initiator, a protein or a protein complex that recognizes the replicator [ 1 ]. (biomedcentral.com)
  • Both processes require genetic variation in adaptive traits within populations. (harvard.edu)
  • Notwithstanding its characterization as an endothelial cell marker, the full spectrum of biological processes associated with ALK1 is essentially unexplored. (lu.se)
  • However, the slow process of in vivo characterization of network function often limits the timescale of the testing step. (biorxiv.org)
  • Collision between replication and transcription is considered especially problematic, as each are catalyzed by large multiprotein machines, and can occur co-directionally, when the replication fork and transcription machinery are moving in the same direction, or on the leading strand and is head-on, when the fork and transcription are moving towards each other. (biomedcentral.com)
  • We implement the controller in an Escherichia coli cell-free transcription-translation system, which allows rapid prototyping and implementation. (nature.com)
  • After more than four decades of intensive research, the cellular origins of acute lymphocytic leukemia (ALL) have been well defined, and several distinct genetic mechanisms that lead to malignant transformation of these cells have been identified ( 1 - 4 ). (aacrjournals.org)
  • Characterization and Mapping of a Salt-Sensitive Mutant in Rice ( Oryza sativa L.) [J]. J Integr Plant Biol. (jipb.net)
  • Genetic analysis was used to characterize the dogĀ“s virus (KU-08), and the sequences were deposited at GenBank under accession number DQ530170-7. (flu.org.cn)
  • Quantitative genetic analysis of chlorophyll a fluorescence parameters in maize in the field environments [J]. J Integr Plant Biol. (jipb.net)
  • In this webinar, we show how prototyping CRISPR components can be accelerated by combining a cell-free transcription and translation (TXTL) system with an Echo Acoustic Liquid Handler (LH) from Beckman Coulter Life Sciences. (beckman.com)
  • We also provide guidelines for designing TX-TL experiments to characterize new genetic networks. (biorxiv.org)
  • Modern genetic techniques have helped us hugely in characterizing these disorders and providing a molecular diagnosis in the face of nonspecific clinical data. (medscape.com)
  • Like other arenaviruses, Lassa virus lacks a ogy and clinical presentation, treatment, prevention conventional negative-strand coding arrangement and control as well as the current theories of its patho- and the isolates of the virus differ in their genetic, genesis and efforts in vaccine development. (folkhalsomyndigheten.se)
  • Clinical and genetic analyses of a Swedish patient series diagnosed with ataxia. (lu.se)
  • The genetic transformation of plants and the subsequent development of transgenic lines with disturbed sugar metabolism have made an unprecedented impact on the study of sugar translocation and -partitioning. (sun.ac.za)
  • Standard protein purification and characterization techniques, enzyme activity assays, as well as immunoassays (ELISA) will also be taught. (uaeu.ac.ae)
  • SIVcpz does not cause an Aids-like illness among chimpanzees, despite its similarity to the human virus and the very close genetic relationship between chimps and humans. (balloon-juice.com)
  • The authors concluded that RFLP-based markers could be used for investigating genetic relationships between maize inbred lines and assigning them to heterotic groups, but it seemed that a large number of PECs were needed to obtain reliable estimates of genetic similarity. (jipb.net)
  • During the last five years, enormous progress has been made in understanding the molecular basis of circadian systems, mainly by molecular genetic studies using the mouse and fly. (bioone.org)
  • Second, an L1 transposon element, which is absent in the human promoter, is found 480 bp upstream of the transcription start site in mouse. (aspetjournals.org)
  • During evolution of plants, transcription factos highly diverified. (fu-berlin.de)
  • Because glucuronoconjugation is the major route of bilirubin elimination, any genetic alteration that affects bilirubin glucuronosyltransferase activity may result in a more or less severe hyperbilirubinemia. (aspetjournals.org)
  • Professor Sharp said: "We are currently working to understand which genetic differences between SIVcpz and HIV-1 evolved as a response to the species jump. (balloon-juice.com)