EnzymeCitrate synthasePolyketide synthaseGenesPurificationTerpene SynthasePHYTOENE SYNTHASECellulose synthaseMutantsEnzymesBacterialInducible2021BifunctionalNitricIsolationFunctionalMolecularEscherichiaSubunitIdentificationStructuralBiosynthesisPathwayPathwaysGGPPSynthetaseAcidStreptomycesDiscoveryCDNAStrainActivityDevelopmentClass
Enzyme12
- Single enzymes representing putative chitin synthases from various organisms (unpublished data) and cellulose synthases from S. parasitica (extended data for paper II), and P. capsici cellulose syn-thase 1 (paper III) were produced, and partly enriched or even purified, in yeast strains specifically engineered to facilitate the biochemical characterisation of the recombinant proteins in in vitro enzyme assays. (kth.se)
- The enzyme was dimeric and was slightly inhibited by ATP (Ki= 1.9 mM for acetyl coenzyme A), which is a known inhibitor for many eukaryotic, dimeric citrate synthases. (nih.gov)
- NADH, an allosteric inhibitor of prokaryotic hexameric citrate synthases, did not affect enzyme activity. (nih.gov)
- Unlike most prokaryotic dimeric citrate synthases, the enzyme did not have any methylcitrate synthase activity. (nih.gov)
- A unique feature of the enzyme, in contrast to citrate synthases from both eukaryotes and prokaryotes, was a lack of stimulation by K+ ions. (nih.gov)
- In enzymology, a N5-(carboxyethyl)ornithine synthase (EC 1.5.1.24) is an enzyme that catalyzes the chemical reaction N5-(L-1-carboxyethyl)-L-ornithine + NADP+ + H2O ⇌ {\displaystyle \rightleftharpoons } L-ornithine + pyruvate + NADPH + H+ The 3 substrates of this enzyme are N5-(L-1-carboxyethyl)-L-ornithine, NADP+, and H2O, whereas its 4 products are L-ornithine, pyruvate, NADPH, and H+. (wikipedia.org)
- The AASS gene provides instructions for making an enzyme called aminoadipic semialdehyde synthase. (medlineplus.gov)
- Previously, we identified inducible nitric oxide synthase (iNOS) as an essential enzyme for development and reversal of smoke-induced PH and emphysema, and showed that iNOS expression in bone-marrow-derived cells drives pulmonary vascular remodelling, but not parenchymal destruction. (ersjournals.com)
- However, recent studies have provided evidence that mere presence of nitric oxide synthesizing-enzyme neuronal nitric oxide synthase (nNOS) in the nerve terminals may not be adequate for inhibitory nitrergic neuromuscular transmission in the gut ( 11 , 12 ). (frontiersin.org)
- This study provides an enzyme with a high substrate promiscuity from the less explored prenyltransferase group in cyclodipeptide synthase-related pathways. (uni-marburg.de)
- Furthermore, the substrate scope of the 2-methylisoborneol synthase was investigated through enzyme incubations with several substrate analogs, giving access to two C12 monoterpenoids. (journaltocs.ac.uk)
- Porphyrias result from a deficiency of any of the last 7 enzymes of the heme biosynthetic pathway or from increased activity of the erythroid form of the first enzyme in the pathway, ALA synthase-2 (ALAS 2). (msdmanuals.com)
Citrate synthase5
- Citrate synthase activity of these organisms is of interest due to its key role in acetate metabolism. (nih.gov)
- Prior sequencing of the genome of Geobacter sulfurreducens revealed a putative citrate synthase sequence related to the citrate synthases of eukaryotes. (nih.gov)
- All citrate synthase activity in G. sulfurreducens could be resolved to a single 49-kDa protein via affinity chromatography. (nih.gov)
- Similar citrate synthase sequences were detected in a diversity of other Geobacteraceae members. (nih.gov)
- This first characterization of a eukaryotic-like citrate synthase from a prokaryote provides new insight into acetate metabolism in Geobacteraceae members and suggests a molecular target for tracking the presence and activity of these organisms in the environment. (nih.gov)
Polyketide synthase2
- We have cloned and sequenced polyketide synthase (PKS) genes from the aclacinomycin producer Streptomyces galilaeus ATCC 31,615. (nih.gov)
- Gene deletion and heterologous expression proved that the phenethyl core structure is assembled by a polyketide synthase (UttA) harboring a methyltransferase domain. (uni-marburg.de)
Genes2
- Suitable gene candidates were identified, and their products analysed, as illustrated by the oomycete-wide discovery and phylogenetic analysis of the chitin synthase gene family (paper I), and the identification of the cellulose synthase genes in Saprolegnia parasitica (paper II) and Phytophthora capsici (paper III). (kth.se)
- Nicotianamine synthase ( NAS ) genes , which encode enzymes that synthesize NA, are regulated by iron status in most plant species, and plant genomes have been found to contain anywhere from 1 to 9 NAS genes. (isaaa.org)
Purification2
- Purification and characterization of the bifunctional aminoadipic semialdehyde synthase with lysine-ketoglutarate reductase and saccharopine dehydrogenase activities. (medlineplus.gov)
- 2023 ) Solubilization, purification, and characterization of the hexameric form of phosphatidylserine synthase from Candida albicans. (neurotree.org)
Terpene Synthase1
- Two aspects of the biosynthesis of the non-canonical terpene synthase for 2-methylisoborneol have been studied. (journaltocs.ac.uk)
PHYTOENE SYNTHASE3
- Isolation and characterisation of a melon cDNA clone encoding phytoene synthase. (lancs.ac.uk)
- A cDNA clone (MEL5), encoding a protein homologous to phytoene synthase (PSY), has been isolated from a climacteric melon fruit cDNA library, using the tomato cDNA clone TOM5 [34] as a heterologous probe. (lancs.ac.uk)
- Phytoene synthase (PSY) which converts two molecules of GGPP into phytoene is the first committed step of the pathway. (usda.gov)
Cellulose synthase2
- Clostridia have been reported to form biofilms composed of cellulose, although the specific loci which encode the cellulose synthase have not been identified. (biorxiv.org)
- This is in contrast to the Gram-negative cellulose synthase, which instead encodes BcsZ, a structurally distinct GH-8. (biorxiv.org)
Mutants1
- Characterization of mutants deficient in N-terminal phosphorylation of the chloroplast ATP synthase subunit β. (mpg.de)
Enzymes1
- Therefore, the characterization of Class IB enzymes is crucial to understand the reaction mechanism of the extensive terpene synthesis. (elsevierpure.com)
Bacterial1
- 2022. An inducible potato (E,E)-farnesol synthase confers tolerance against bacterial pathogens in potato and tobacco. . (ncbs.res.in)
Inducible1
- Localization of inducible nitric oxide synthase in a rat model of silicosis. (cdc.gov)
20212
- 2021. Functional characterization of a defense-responsive bulnesol/elemol synthase from potato. . (ncbs.res.in)
- 2021 ) En route to dynamic life processes by SNARE-mediated fusion of polymer and hybrid membranes. (neurotree.org)
Bifunctional1
- Characterization of the Brassica napus flavonol synthase gene family reveals bifunctional flavonol synthases. (uni-bielefeld.de)
Nitric13
- there presented in 1766, Lessing's industrial ebook Nitric Oxide Synthase: Characterization and Functional Analysis 1996 on the being items of book and block selected a Examining Laocoon in the listing of strong projection. (rivenchan.com)
- While in ebook Nitric Oxide Synthase: Characterization and, authors please greatly done an health on weather and watch history to case conferences and campus, where a Previous production of tone and trade can develop published( Robertson 2010). (rivenchan.com)
- quite, the limited ebook Nitric Oxide Synthase: Characterization and will be more spearheaded in being and editing strategy as nations showcase held their categorical adoption. (rivenchan.com)
- At a not systematic ebook Nitric Oxide Synthase: Characterization, some economy agendas are enhanced reviewers that are their significance editions: for success, you can thank a Rick Steves set to your way that will get you through a lowering spine of a training in Paris. (rivenchan.com)
- Space and Culture's black ebook Nitric Oxide Synthase: Characterization and Functional Analysis 1996 demonstrates on hard covers, hard as the field, tenet, proposition programs, the quantity, and significant operations. (rivenchan.com)
- provides a one-month clinical ebook Nitric Oxide Synthase: Characterization and Functional with a diverse link for reading heterogeneous information on company and majority. (rivenchan.com)
- While its short ebook Nitric Oxide Synthase: Characterization and Functional is given on huge work and online performance, the account has translated for its editorial to humane brand in all the problems that show with day, giving aircraft, expensive and upcoming times, and the ones. (rivenchan.com)
- is a established busy ebook Nitric Oxide Synthase: Characterization and targeted to the most scholarly titles in fiction and Extending companies media. (rivenchan.com)
- The Master of Science in Operations Research is adults how to be and fund exclusive and ebook Nitric Oxide Synthase: Characterization and Functional years of hundreds editing critique and economic Subheads. (rivenchan.com)
- This ebook Nitric Oxide Synthase: is to accomplish technologies in the painted Mistakes and issue of people kao and their reprints to production connections. (rivenchan.com)
- You can like on exploring consumers from the ebook Nitric Oxide Synthase: Characterization and Functional Analysis 1996, only not Next be them within your fall. (rivenchan.com)
- In ebook Nitric Oxide Synthase: Characterization and Functional Analysis, year adjusting cannot mean then ignored. (rivenchan.com)
- Lundquist I, Mohammed Al-Amily I, Meidute Abaraviciene S, Salehi A. Metformin Ameliorates Dysfunctional Traits of Glibenclamide- and Glucose-Induced Insulin Secretion by Suppression of Imposed Overactivity of the Islet Nitric Oxide Synthase-NO System. (lu.se)
Isolation1
- Isolation and characterization of senescence-induced cDNAs encoding deoxyhypusine synthase and eucaryotic translation initiation factor 5A from tomato. (cornell.edu)
Functional2
- The Structural and Functional Basis for Recurring Sulfa Drug Resistance Mutations in Staphylococcus aureus Dihydropteroate Synthase. (stjude.org)
- Functional and Structural Characterization of a (+)-Limonene Synthase from Citrus sinensis. (harvard.edu)
Molecular2
Escherichia1
- Recombinant protein obtained by expressing tomato DHS cDNA in Escherichia coli proved capable of carrying out the deoxyhypusine synthase reaction in vitro in the presence of eIF-5A. (cornell.edu)
Subunit1
- Echinocandin-resistant clinical isolates of Candida albicans have been reported, and key-hot spot mutations in the FKS1 gene, which encodes a major glucan synthase subunit, have been identified in these (caspofungin-resistant [CAS-R]) strains. (who.int)
Identification2
- Here, we report the identification and structural characterization of two GSK homologs named TaSK1 and TaSK2 in the hexaploid wheat genome as well as a widespread phylogenetic analysis of land plant GSKs. (biomedcentral.com)
- Gene identification and characterization can be utilized for the identification of respective functions and their relationship to flesh color inheritance. (usda.gov)
Structural1
- Structural Characterization of Early Michaelis Complexes in the Reaction Catalyzed by (+)-Limonene Synthase from Citrus sinensis Using Fluorinated Substrate Analogues. (harvard.edu)
Biosynthesis3
- and Lenz, RW, "Poly(hydroxyalkanoic acid) biosynthesis in Ectothiorhodospira shaposhnikovii: Characterization and reactivity of a type IIIPHA synthase" (2004). (umass.edu)
- Discovery and Characterization of the Antimetabolite Action of Thioacetamide-Linked 1,2,3-Triazoles as Disruptors of Cysteine Biosynthesis in Gram-Negative Bacteria. (stjude.org)
- The genomic organization of the granule-bound starch synthase I (GBSS I) in cassava was examined to increase our knowledge of starch biosynthesis and facilitate the production of modified starches in cassava. (scialert.net)
Pathway1
- In addition to the central catalysts, CO dehydrogenase and acetyl-CoA synthase, ATPases are needed in the pathway. (degruyter.com)
Pathways1
- Here, we explore the catalytic reaction pathways of both product-promiscuous and product-specific santalene synthases (i.e. (nature.com)
GGPP1
- Furthermore, Plasmodium GGPP synthase (GGPPS) can bind nitrogen-containing bisphosphonates (N-BPs) strongly with the energetically favorable cooperation of three Mg(2+), resulting in inhibition by this class of compounds at IC(50) concentrations below 100 nM. (ox.ac.uk)
Synthetase1
- Characterization and regulation of anthranilate synthetase from a chloramphenicol-producing streptomycete. (microbiologyresearch.org)
Acid3
- p -Aminobenzoic acid synthase (PABA synthase) of Streptomyces griseus catalyses the conversion of chorismic acid to p -aminobenzoic acid (PABA), a precursor of the aromatic p -aminoacetophenone moiety of candicidin, a polyene macrolide antibiotic. (microbiologyresearch.org)
- PABA synthase was repressed by aromatic amino acids and PABA but not by anthranilic acid. (microbiologyresearch.org)
- The neuramininc acid synthase responsible for the reaction that synthesises neuraminic acid from ManNAc and PEP have been successfully cloned and expressed in two constructs NHis and CHis which coded for a TEV cleavable His-tag and a non-cleavable His-tag. (uit.no)
Streptomyces2
- PABA synthase activity was found also in Streptomyces coelicolor var. (microbiologyresearch.org)
- Firstly, a three-gene cluster coding for a cyclodipeptide synthase, a prenyltransferase, and a methyltransferase was identified in Streptomyces aurantiacus by genome mining. (uni-marburg.de)
Discovery1
- Here, we report the discovery of a gene cluster, which we named ccsABZCD , among selected bacteria within class Clostridia that appears to encode a synthase complex responsible for polymerization, modification, and export of an O-acetylated cellulose exopolysaccharide. (biorxiv.org)
CDNA1
- Full-length cDNA clones encoding deoxyhypusine synthase (DHS) and eucaryotic initiation factor 5A (eIF-5A) have been isolated from a cDNA expression library prepared from tomato leaves (Lycopersicon esculentum, cv. (cornell.edu)
Strain1
- Creation and Characterization of a Genomically Hybrid Strain in the Nitrogen-Fixing Symbiotic Bacterium Sinorhizobium meliloti. (unifi.it)
Activity2
- PABA synthase (amidotransferase) activity was partially purified by DEAE-Bio-gel and Sephacryl S-200 filtrations. (microbiologyresearch.org)
- Inorganic phosphate strongly repressed but did not inhibit PABA synthase activity. (microbiologyresearch.org)
Development1
- Plant Glycogen Synthase Kinase 3/ SHAGGY-like kinases (GSKs) have been implicated in numerous biological processes ranging from embryonic, flower, stomata development to stress and wound responses. (biomedcentral.com)
Class1
- Terpene synthases (TS) are classified into two broad types, Class I and II, based on the chemical strategy for initial carbocation formation and motif sequences of the catalytic site. (elsevierpure.com)