• Aided by molecular modelling, amino acids in the predicted phosphoinositide binding pocket of dynamin and Btk PH domains were selectively mutated in order, to identify key ligand recognition residues. (ucl.ac.uk)
  • In Escherichia coli , amino acid starvation leads to the accumulation of an unusual molecule, polyphosphate (polyP), a linear polymer of many hundreds of orthophosphate residues. (go.jp)
  • The first 84-amino acid residues correspond to a typical transit sequence for the signal directing OEE1 to its appropriate compartment of chloroplast. (biu.ac.il)
  • The third difference is the very high proportion of proline residues in the sequence accounting for about one-third of the sequence. (springer.com)
  • We have sequenced so far 29 amino acid residues of the SQR NH2 terminus and found that from the second residue, this sequence contains the highly conserved fingerprint of the NAD/FAD-binding domain of many NAD/FAD-binding enzymes (Wierenga, R. K., Terpstra, P., and Hol, W. G. S. (1986) J. Mol. (agri.gov.il)
  • The N-terminal region has been predicted to fold into a beta-propeller domain composed of seven beta-sheets each about 60 amino acid residues long, with the I-domain inserted between beta-sheets 2 and 3. (embl.de)
  • The fACCD were found 16.18-82.47 kDa proteins having 149-750 amino acid residues. (springeropen.com)
  • The ORF contained 354bp and encoded 117 amino acid residues, which shares 69% overall amino acid sequenceidentities with the subunit VII of ubiquinol-cytochrome C reductase from bovine. (researchgate.net)
  • The metal ligands and active site residues of extradiol dioxygenases are conserved although several amino acid residues found exclusively in enzymes which preferentially cleave bicyclic substrates are missing in BphC_JF8. (muni.cz)
  • Based on the genome sequencing results, an analysis of structural proteins and prediction of putative microRNAs of Hz-2V was performed. (umass.edu)
  • AbiQ has no homology to known or deduced proteins in the databases. (omicsdi.org)
  • Amino acid sequence homology with proteins in the hsp-70 family. (ocl-journal.org)
  • Structural and biochemical characterisation of Pleckstrin Homology domains Pleckstrin Homology (PH) domains are modules of approximately 100-120 amino-acids found in a variety of proteins involved in intracellular signalling cascades. (ucl.ac.uk)
  • To solve this dilemma, cells must use their own proteins as sources of amino acids in response to the nutritional downshift. (go.jp)
  • Molecular characterization of Xenopus laevis DP proteins. (ox.ac.uk)
  • The deduced amino acid sequences encoded by sepABC show a high homology to bacterial proteins known to be involved in solvent efflux or multidrug pumps. (canada.ca)
  • We identified zebrafish and Fugu TNFSF 13b homologues by genomic DNA database searches and determined that the predicted proteins share 68% and 76% sequence identity with trout TNFSF 13b. (usda.gov)
  • PPR proteins have in common a canonical P-type 35 amino acid domain repeated in tandem up to 30 times. (frontiersin.org)
  • Given the availability of 3D structures of these homologous proteins, the prediction of PAG structure from its amino acid sequence at high confidence levels is implicit. (biomedcentral.com)
  • The present study unveils the structure-function and evolutionary aspects of 1-aminocyclopropane-1-carboxylic acid deaminase (ACCD) proteins of fungal origin. (springeropen.com)
  • Previously, the genome of the cereal pathogen Fg was sequenced and shown to encode a larger number of proteins in pathogenicity related protein families compared to non-pathogenic fungi, including predicted transcription factors, hydrolytic enzymes, and transmembrane transporters 5 . (nature.com)
  • Continued new insights into host-parasite interactions at the molecular level will be achieved by combining clonal analysis of bacterial pathogens with large-scale comparative sequencing of virulence genes. (cdc.gov)
  • Homologies were found to 38 of the 113 ORFs predicted for genes with known functions. (umass.edu)
  • A putative late gene promoter motif sequence, AGTAT was found in the 3' upstream sequences of these genes. (umass.edu)
  • Here you can find the number of classified homology groups and genes on a pangenome level but also for individual genomes. (readthedocs.io)
  • Five Dmrt family genes with full-length transcript sequences were identified in Amur sturgeon. (researchsquare.com)
  • Southern hybridization experiments and analysis of the P. putida KT2440 genome sequence indicated that sepR is a relatively rare commodity compared to homologues of the sepABC genes. (canada.ca)
  • METHODS: To identify expressed TNFSF genes, we searched a computer database of ~50,000 rainbow trout expressed sequence tags generated at the National Center for Cool and Cold Water Aquaculture. (usda.gov)
  • Three introns were identified in the rainbow trout, Atlantic salmon and zebrafish genomic sequences, and the intron locations correspond closely to those in the mammalian TNFSF 13b genes. (usda.gov)
  • Approximately 20% of LTR retrotransposon sequences lie within putative genes. (biomedcentral.com)
  • The male sterilizing factors are produced by recombination of the mitochondrial genome and consist of unusual genes or open reading frames (ORFs) that usually contain a portion of functional mitochondrial genes and sequences of unknown origin ( Hanson and Bentolila, 2004 ). (frontiersin.org)
  • Genes adjacent to pvtramp were identified in silico to determine the degree of similarity between the protein sequences encoded by equivalent chromosomic fragments in P. falciparum and Plasmodium knowlesi . (biomedcentral.com)
  • a , Maximum-likelihood tree using concatenated protein sequences of 100 genes randomly selected from 4,694 Fusarium orthologous genes that have clear 1:1:1:1 correlation among the Fusarium genomes and have unique matches in Magnaporthe grisea , Neurospora crassa and Aspergillus nidulans . (nature.com)
  • Twenty putative pre-microRNA sequences were predicted in Hz-2V by searching stem-loop structures with less than -25kcal/mol of free energy, and one of the microRNA candidates was predicted to target the Hz2V007 gene transcript, which codes for a putative juvenile hormone esterase. (umass.edu)
  • Sequence analysis of this fragment revealed a complete open reading frame (abiQ), which encodes a putative protein of 183 amino acids. (omicsdi.org)
  • Although more than 90 putative PH domains have been identified by sequence analysis, a universal function for PH domains has remained elusive. (ucl.ac.uk)
  • By using sequence alignment of herpesviruses homologs, we identified conserved amino acids in the putative pUL105 ATP binding site and in the putative pUL70 zinc finger pattern. (frontiersin.org)
  • Wamucho, Anye, "Cloning, Heterologous Expression in Yeast, and Biochemical Characterization of Recombinant Putative Glucosyltransferase Clones 9 and 11 from Grapefruit (Citrus paradisi)" (2012). (etsu.edu)
  • We show that the mouse putative pS2 protein sequence and the physiological pattern of expression of the mouse pS2 gene are well conserved. (xenbase.org)
  • Here, we present the complete genome sequences of two P335-type phages, Q33 and BM13, isolated in North America and representing a novel lineage within this phage group. (omicsdi.org)
  • The primary amino acid sequence of a protein is a translated version from its gene sequence which carries important messages and information concealed therein. (springeropen.com)
  • Analysis of the protein sequences of the three CpHsps indicated the presence of 83 amino acids with homology to the a-crystallin domain. (usda.gov)
  • Octameric enolase from the hyperthermophilic bacterium Thermotoga maritima: purification, characterization, and image processing. (uni-regensburg.de)
  • 1997. Functional characterization and purification of an intracellular vitamin D-binding protein in vitamin D-resistant new world primate cells. (ocl-journal.org)
  • 2010) Purification and characterization of a thermostable keratinolytic serine alkaline proteinase from Streptomyces sp. (scielo.br)
  • Biological and structural characterization of glycosylation on ephrin-A1, a preferred ligand for EphA2 receptor tyrosine kinase. (wakehealth.edu)
  • This study describes the structural characterization of a totally new family of peptides from the venom of the snake green mamba ( Dendroaspis angusticeps) . (springer.com)
  • The last two features have made the structural characterization of the new peptides by mass spectrometry a real analytical challenge. (springer.com)
  • The N-terminal 25 amino acids of the enzyme reveal a high degree of similarity to enolases from other sources. (uni-regensburg.de)
  • The matk cDNA clone encodes a polypeptide of 527 amino acids and has closest sequence similarity to the csk tyrosine kinase. (ox.ac.uk)
  • Computer based sequence similarity searches of the nucleic acid databases are considered to be a more accurate method to identify retrotransposon families and estimate TE content and distribution in rice genome [ 28 - 31 ]. (biomedcentral.com)
  • Isolation and characterization of an angiotensin converting enzyme substrate from vitellogenic ovaries of Neobellieria bullata. (ox.ac.uk)
  • We here report the isolation and characterization of the first ACE interactive compound hereof. (ox.ac.uk)
  • Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. (xenbase.org)
  • Molecular cloning and characterization of the mouse tag7 gene encoding anovel cytokine. (embl-heidelberg.de)
  • In this paper we describe the cloning and sequencing of the SWI5 gene. (ox.ac.uk)
  • Glutamate-287 (E287) of murine mature ferrochelatase is a conserved residue in all known sequences of ferrochelatase, is present at the active site of the enzyme, as inferred from the Bacillus subtilis ferrochelatase three-dimensional structure, and is critical for enzyme activity. (unl.pt)
  • Confirmation that the OXA-2 beta-lactamase is a serine enzyme has come from the DNA sequence and the interaction with mechanism based inactivators. (bl.uk)
  • Characterisation of the physical properties of the OXA-2 enzyme by analytical ultracentrifugation have not only confirmed the dimeric nature of this beta-lactamase but have also shown that this enzyme forms aggregates at high protein concentrations. (bl.uk)
  • A 3-D homology model of BphC_JF8 provided a basis for understanding the substrate specificity, quaternary structure and stability of the enzyme. (muni.cz)
  • Bacterial artificial chromosome clones have been isolated from a trout library to further facilitate genomic characterization. (usda.gov)
  • We have recently identified a new class of enzymes, Class IB, showing the acceptability of long (C20-C35) prenyl-diphosphates as substrates and no amino acid sequence homology with known TS. (elsevierpure.com)
  • Initial screens show catechol, gentisic acid, vanillin, and p-hydroxylphenylacetic acid as potential substrates for the PGT 9 protein. (etsu.edu)
  • In this work, a general biochemical characterization in snake venom of serine proteases (SVSP), phospholipases A 2 (PLA 2 ), metalloproteases (SVMP) and hyaluronidases (SVH) of Crotalus aquilus (Ca), Crotalus polystictus (Cp) and Crotalus molossus nigrescens (Cmn) was done. (mdpi.com)
  • and (iii) an intracellular signal-transductor of the Smad family (1530 bp cDNA, 318 amino acids) could be identified and completely characterized. (uni-wuerzburg.de)
  • Pharmacology of a new porcine pancreatic polypeptide with spasmolytic and gastric acid secretion inhibitory effects. (xenbase.org)
  • Thyroxine-binding globulin (TBG) is a 395-amino acid, 54kd polypeptide that is synthesized in the liver and encoded by a single gene copy. (medscape.com)
  • Phylogenetic tree showed Bmuccr hadhigh homology with T. castaneum homologous. (researchgate.net)
  • Peptides were characterized by a combined use of MALDI- TOF/TOF and nanoESI-IT-ETD experiments to determine not only the peptide sequence but also the composition and the position of the carbohydrate moiety. (springer.com)
  • This 1312.7 Da peptide with the sequence NKLKPSQWISL, is substrate to both insect and human ACE. (ox.ac.uk)
  • We have previously shown that the human pS2 gene, which codes for a secreted peptide of 60 amino acids, is expressed in a number of human carcinomas, including carcinomas of the breast, the pancreas , and the large bowel. (xenbase.org)
  • Columns for features like exon and intron will remain empty as bacterial coding sequences are not interrupted. (readthedocs.io)
  • Characterization of a transcription factor involved in mother cell specific transcription of the yeast HO gene. (ox.ac.uk)
  • The predicted amino acid sequence derived from the cloned SWI5 gene shows homology with the repeated DNA-binding domains ('zinc fingers') of Xenopus transcription factor TFIIIA. (ox.ac.uk)
  • Protein chemical characterization of Mucor pusillus aspartic proteinase. (wikipedia.org)
  • Structural and functional characterization of monomeric EphrinA1 binding site to EphA2 receptor. (wakehealth.edu)
  • Although homology-modeled structures may often not be accurate enough to allow characterization of protein-protein or protein-inhibitor interactions at the atomic level, they can suggest which sequence regions or individual amino acids are essential functional components of the protein. (biomedcentral.com)
  • In B. gymnorrhiza, the predicted amino acid sequence of the mature protein starts at the residue number 85 of the open reading frame. (biu.ac.il)
  • The Q33 and BM13 genomes exhibit homology, not only to P335-type, but also to elements of the 936-type phage sequences. (omicsdi.org)
  • A PSI-BLAST (Position Specific Iterated-Basic Local Alignment Search Tool) [ 7 ] search with default parameters was performed against the Protein Data Bank (PDB) to find a suitable template for homology modeling. (biomedcentral.com)
  • The multiple sequence alignment of 16 subunit VII homologues showsthat Bmuccr is very hydrophilic, has a characteristic charge distribution, and has a high helical content. (researchgate.net)
  • 2004) Characterization of a protease of a feather-degrading Microbacterium species. (scielo.br)
  • In order to identify P450UK, it was purified and subjected to amino-terminal amino acid analysis. (nih.gov)
  • Two transmembrane domains were predicted in p11.7, and a DNA binding motif was found in p31.7 by amino acid sequence analysis, therefore p11.7 and p31.7 were predicted to be envelope and capsid protein, respectively. (umass.edu)
  • Mutational analysis of several of these amino acids both in pUL105 and pUL70, proved that they are crucial for viral replication. (frontiersin.org)
  • For example, in an environment rich in amino acids, cells do not expend energy for making amino acid biosynthetic enzymes. (go.jp)
  • However, if the environment becomes depleted of amino acids (nutritional downshift), cells will be exposed to a lack of both the amino acid biosynthetic enzymes and the amino acids required to make these enzymes. (go.jp)
  • Once amino acid biosynthetic enzymes start to accumulate, the cell is able to produce its own amino acids, and a new growth phase begins. (go.jp)
  • Therefore, the characterization of Class IB enzymes is crucial to understand the reaction mechanism of the extensive terpene synthesis. (elsevierpure.com)
  • Expression and pharmacological characterization of a canine 5-hydroxytryptamine1D receptor subtype. (aspetjournals.org)
  • RDC4 is an intronless gene encoding a protein of 377 amino acids, which exhibits greatest sequence identity (43%) to the 5-HT1A receptor and lower overall homology to other serotonergic and catecholaminergic receptors. (aspetjournals.org)
  • We also constructed, by homology modeling, a theoretical structure of the pUL105 N-terminal domain which indicates that the mutated conserved amino acids in this domain could be involved in ATP hydrolysis. (frontiersin.org)
  • The physiological effects of ROS include amino acid chain cleavage, DNA degradation and lipid oxidation, among others. (unl.pt)
  • according to morphological, physiological and its 16S rRNA gene sequence. (researchgate.net)
  • In order to gain insight into the chromosomal distribution of LTR-retrotransposons in the rice genome, a detailed examination of LTR-retrotransposon sequences on Chromosome 10 was carried out. (biomedcentral.com)
  • Terpene synthases (TS) are classified into two broad types, Class I and II, based on the chemical strategy for initial carbocation formation and motif sequences of the catalytic site. (elsevierpure.com)
  • RESULTS: Here, we report the characterization of a cDNA, 1RT126I22, which contains a TNF homology domain. (usda.gov)
  • The PfTRAMP homologue in P. vivax , here denoted as PvTRAMP, is a 340-amino-acid long antigen encoded by a single exon that could have a potential role in cytoadherence, as indicated by the presence of a thrombospondin structural homology repeat (TSR) domain. (biomedcentral.com)
  • In canine epidermolysis bullosa acquisita, the immunoglobulin G (IgG) autoantibodies also target the type VII collagen noncollagenous (NC1) domain, which shares greater than 80% homology in amino acid sequence with the human NC1 domain. (medscape.com)
  • However, it is difficult to capture the complexity of transcript isoform variance using these libraries, and large cDNA sequences are often difficult to clone into size-limited viral expression vectors. (cdc.gov)
  • PGT 11 has been successfully cloned into pPICZA for transformation into yeast, expression, and subsequent characterization. (etsu.edu)
  • The primary structure of BphC_JF8 exhibits less than 25% sequence identity to other 2,3-dihydroxybiphenyl 1,2-dioxygenases. (muni.cz)
  • Sequence of the pS2 mRNA induced by estrogen in the human breast cancer cell line MCF-7. (xenbase.org)
  • We determined the sequence of pig Reelin mRNA and protein and identified a high degree of homology to human Reelin. (biomedcentral.com)
  • X: any amino acid except Pro) present in newly synthesized protein in yeast and mammalian cells. (elifesciences.org)
  • Sequence comparisons also indicate that matk contains src homology region 2 and 3 domains but lacks the NH2-terminal myristylation signal, the negative regulatory tyrosine (Tyr-527), and the autophosphorylation site (Tyr-416) corresponding to those found in src. (ox.ac.uk)
  • In nutrient-poor medium a wider range is found, including unsaturated fatty acids, which are absent when a nutrient-rich medium is used. (microbiologyresearch.org)
  • The trout TNFSF 13b predicted protein shares 54% amino acid identity with human and chicken TNFSF 13b, and 51% identity with mouse TNFSF 13b. (usda.gov)
  • Estrogen-responsive element of the human pS2 gene is an imperfectly palindromic sequence. (xenbase.org)
  • The acyl lipids in strain 1/15 contain predominantly branched-chain fatty acids, mainly anteiso-15:0. (microbiologyresearch.org)
  • The acyl lipids of strain 2/10 contain 16:0 and 16:1 as the major fatty acids, particularly in rich medium, when they comprise >90% of the total. (microbiologyresearch.org)
  • We sequenced Fv strain 7600 and Fol strain 4287 (Methods, Supplementary Table 1 ) using a whole-genome shotgun approach and assembled the sequence using Arachne ( Table 1 , ref. 6 ). (nature.com)
  • The amino-terminal sequence obtained for P450UK was identical to the sequence deduced from a cDNA encoding CYP2C19, thus identifying P450UK as 2C19. (nih.gov)
  • The deduced amino acid sequence consisted of 322 amino acids and was 87% identical to that of Nicotiana tabacum. (biu.ac.il)