Characterization of antibody antigen complexes. Medical search. Frequent questions

These antibodies have been verified by Cell treatment to confirm specificity to MHC Class II (I-A/I-E). Find the MHC Class II (I-A/I-E) antibody that fits your needs. (thermofisher.com)
Antibodies with Advanced Verification data have been validated for specificity to ensure that the antibody binds to the antigen stated. (thermofisher.com)
The genetic transfer of T-cell receptors (TCRs) directed toward target antigens into T lymphocytes has been used to generate antitumor T cells efficiently without the need for the in vitro induction and expansion of T cells with cognate specificity. (fujita-hu.ac.jp)
The specificity of tissue antigens has previously been investigated by the usual immunologic procedures [1] and the effects of antibodies [2,3] upon tissue explants has long been known. (caltech.edu)
The growth and function of tissue explants have previously been used to study the specificity of tissue antibodies [4,5], but the antisera used were of low, uncertain potency, and only a few different tissues were examined in testing the toxic effects. (caltech.edu)
The specificity for and function of monoclonal antibodies against Tritrichomonas foetus were characterized. (docksci.com)
Therapeutics of this nature capitalize on the exquisite specificity of antibody biologics to directly target tumor cells with cytotoxic agents (for example, ADCs) or indirectly target tumor cells by actively recruiting other immune cells (such as T-cells and macrophages) via multi-specific antibody formats. (news-medical.net)
Our traditional hybridoma discovery approach triages antibody candidates in a high-throughput manner by specificity, affinity, and oftentimes functionality early in the workflow. (news-medical.net)
While all known superantigens induce vast immune cell proliferation and come from opportunistic pathogens, recently, proteins with similar broad specificity to antibody variable (V) domain families were identified in a commensal microbiota. (cornell.edu)
The polyclonal antibody E053 recognizes the cytoplasmic part of the epsilon subunits of the CD3 complex on thymocytes and on peripheral T lymphocytes and, in contrast to many monoclonal antibodies of the same specificity, is suitable for cryostat material and kerosene sections. (histoprime.com)
A judicious alternative to chemical molecules and small peptides with short half-life is the monoclonal antibody where high levels of specificity would be expected to mitigate off-target effects, and generate safer and more potent classes of drugs. (synabs.be)
Competitive assays play a vital role in antibody characterization due to their ability to provide valuable insights into the binding properties, specificity, and stability of antibodies. (iaanalysis.com)
The development of antibodies for diagnostics or therapeutics requires comprehensive characterization of affinity, specificity and mechanism of action. (technologynetworks.com)
In this approach a nonradiolabeled bispecific monoclonal antibody (bs-mAb) with antitumor-associated antigen (TAA) and antihapten specificity is injected in the first phase. (snmjournals.org)

The few research laboratories with rare, coveted and well sheltered polyclonal antisera were joined by many laboratories freely sharing their monoclonal antibody resources and associated data. (karger.com)
Anti-CD9 monoclonal antibody activates p72syk in human platelets. (bdbiosciences.com)
Monoclonal antibody TF3.8 recognized a predominant band at -155 kilodaltons, whereas TF3.2 reacted with several bands. (docksci.com)
Monoclonal antibody production. (docksci.com)
The same region of A3Bctd was used previously as a peptide antigen for generating a rabbit monoclonal antibody (mAb 5210-87-13), suggesting that this region is particularly immunogenic and that these antibodies from very different origins may share similar binding modes. (uthscsa.edu)
Tumors were pretargeted with the bispecific monoclonal antibody (bs-mAb) G250xDTIn-1 and, 72 h later, mice were injected intravenously with one of both radiolabeled peptides. (snmjournals.org)

The capture molecules arrayed on the solid surface may be antibodies, antigens, aptamers (nucleic acid-based ligands), affibodies (small molecules engineered to mimic monoclonal antibodies), or full length proteins. (wikipedia.org)
Quantification and characterization of exosomal proteins is then performed using appropriate detection antibodies against exosome-associated antigens, which can be either generic or cell / tissue specific exosomes. (immpact-international.org)
We sought to determine if there was antibody deposition in SAH livers and whether antibodies extracted from SAH livers were cross-reactive against both bacterial antigens and human proteins. (elifesciences.org)
Employing human proteome arrays, we profiled the antibodies extracted from explanted SAH, alcoholic cirrhosis (AC), nonalcoholic steatohepatitis (NASH), primary biliary cholangitis (PBC), autoimmune hepatitis (AIH), hepatitis B virus (HBV), hepatitis C virus (HCV) and HD livers and found that antibodies of IgG and IgA isotypes were highly accumulated in SAH and recognized a unique set of human proteins as autoantigens. (elifesciences.org)
This important study tested the hypothesis that liver-derived but not serum-derived antibodies that are cross-reactive to E.coli and to host proteins can play a role in the hepatic damage found in severe alcoholic hepatitis (SAH). (elifesciences.org)
Antibodies are heavy (~150 k Da ) proteins of about 10 nm in size, [7] arranged in three globular regions that roughly form a Y shape. (wikipedia.org)
Invasion of human erythrocytes by Plasmodium falciparum (Pf) merozoites relies on the interaction between two parasite proteins: apical membrane antigen 1 (AMA1) and rhoptry neck protein 2 (RON2). (bvsalud.org)
Newly produced antibody, like the other plasma proteins, became readily labelled with S35 amino acids within a few hours. (caltech.edu)
Some success has been obtained also in the formation of antibodies from other serum proteins and by other denaturation-renaturation procedures. (caltech.edu)
Although the idea of generating therapeutic monoclonal antibodies that block or agonize ion channels, which are membrane proteins, seems to be self-evident, its implementation is more complicated than expected. (synabs.be)
However, suitable viroporin-targeting antibodies are difficult to generate because of the well-recognized technical challenge associated with isolating antibodies to complex transmembrane proteins. (bvsalud.org)
Isolation and characterization of proteins from Rous sarcoma virus. (microbiologyresearch.org)
Previously, we've reported a fluorescent dye-conjugated antibody focusing on cancer of the colon secreted proteins-2 (CCSP-2), a proteins indicated in colorectal adenoma and adenocarcinoma cells extremely, enable you to distinguish tumor lesions and regular cells with fluorescent indicators that may be recognized by former mate vivo molecular imaging24. (p38-mapk-inhibitors.com)
Biosensors with different ligands that bind antibodies, proteins and small molecules such as streptavidin, human Fc, mouse Fc, protein A, anti-his, and Ni-NTA are routinely used. (technologynetworks.com)
We provide additional C1q binding assays to support the production, process development, characterization, and similarity studies of therapeutic antibodies and Fc fusion proteins. (party.biz)

Genetically engineered T cells expressing a chimeric antigen receptor (CAR) are rapidly emerging a promising new treatment for haematological and non-haematological malignancies. (nature.com)
Alternatively, T cells have been gene-modified with a TCR-like antibody or chimeric antigen receptor (CAR). (fujita-hu.ac.jp)
To advance vaccine development, here we engineered chimeric antigens by replacing the AMA1 DII loop, displaced upon ligand binding, with RON2L. (bvsalud.org)
Here, we aimed to overcome this limitation by engineering NK cells to express chimeric antigen receptors (CARs) to boost their anti-AML activity and interleukin (IL)-15 to enhance their persistence. (bvsalud.org)
Chimeric rabbit/human Fab antibodies against the hepatitis Be-antigen and their potential applications in assays, characterization, and therapy. (nih.gov)
Over the last decade, chimeric antigen receptor (CAR) T-cell therapies have attracted growing interest for their potential to treat a wide range of cancers. (perkinelmer.com)
To enhance the immunogenicity of this protein, which has a low molecular weight, the aim of the present work was to express, isolate, and characterize a chimeric protein based on the fusion of the bacterial chaperone HSP70 of Yersinia pseudotuberculosis and EIII (DIII + stem) as a prospective antigen for an adjuvanted delivery system, the tubular immunostimulating complex (TI-complex). (usf.edu)
ELISA, differential scanning calorimetry, intrinsic fluorescence, and computational analysis were applied for the characterization of the immunogenicity and conformation of the chimeric protein. (usf.edu)
Mice immunization showed that the chimeric protein induced twice the number of anti-EIII antibodies in comparison with EIII alone. (usf.edu)
In turn, the incorporation of the HSP70/EIII chimeric protein in the TI-complex resulted in a twofold increase in its immunogenicity. (usf.edu)
Using HSP70 in the content of the chimeric protein represents an efficient means for presenting the main antigenic domain of the TBEV envelope protein to the immune system, whereas the incorporation of this chimeric protein into the TI-complex further contributes to the development of a stronger immune response against the TBEV infection. (usf.edu)

The remaining four directed against a second epitope gave significant persistent fractions which could be reduced by addition of complement, anti-mouse immunoglobulin, or antibody 2.25.4. (microbiologyresearch.org)
An antibody ( Ab ), also known as an immunoglobulin ( Ig ), [1] is a large, Y-shaped protein used by the immune system to identify and neutralize foreign objects such as pathogenic bacteria and viruses . (wikipedia.org)
Antibodies are glycoproteins belonging to the immunoglobulin superfamily . (wikipedia.org)
The terms antibody and immunoglobulin are often used interchangeably, [1] though the term 'antibody' is sometimes reserved for the secreted, soluble form, i.e. excluding B-cell receptors. (wikipedia.org)
Undegraded myelin is initially enveloped by membranes bearing fixed complexes of immunoglobulin and complement. (nih.gov)
Anti-GBM antibodies are almost exclusively of the immunoglobulin G (IgG) isotype. (medscape.com)
The activation of the C1 complex (consisting of a single C1q molecule and two C1r and C1s molecules) that occurs when C1q binds to multiple immunoglobulin (IgG) molecules complexed with an antigen is highlighted by the C1q binding assay. (party.biz)

Complex macromolecules, such as immunoglobulins, or genetic material often cannot be adequately characterized by chemical or physical means alone, and reference preparations are necessary to enable consistency and comparison across assays. (cdc.gov)
The main focus of our research group is the structural, biophysical and immunological characterization of antibody-antigen complexes by a multidisciplinary approach ranging from experimentally validated structural bio-computing to in cell infectivity assays. (usi.ch)
We leverage a number of proprietary immunization strategies for tough target antibody discovery along with a suite of premium downstream characterization assays to ultimately deliver a panel of fully characterized lead molecules ready for development. (news-medical.net)
The crystal structure and biochemical assays of a truncated IbpA construct in complex with mouse VH5 antigen-binding fragment (Fab) shows a binding of Ig heavy chain framework residues to the Ibp Domain D and the C-terminal heavy chain binding domain (HCBD). (cornell.edu)
Why Competitive Assays in Antibody Characterization? (iaanalysis.com)
By introducing competition between multiple antibodies for the same antigen, these assays allow for a comprehensive analysis of their binding affinities, epitope mapping, and functional characterization. (iaanalysis.com)
Furthermore, competitive assays enable researchers to evaluate the potential cross-reactivity of antibodies, assess their suitability for specific applications, and compare the performance of different candidates. (iaanalysis.com)
By employing competitive assays, scientists can make informed decisions and ensure the successful development of safe and effective antibody-based therapeutics. (iaanalysis.com)
Competitive assays enable the quantification of binding affinities between antibodies and target antigens. (iaanalysis.com)
Competitive assays aid in the identification and mapping of epitopes recognized by antibodies. (iaanalysis.com)
Competitive assays, including nano-differential scanning fluorescence (nanoDSF) , allow assessment of the thermal stability of antibodies. (iaanalysis.com)
These binders were subjected to further characterization using enzyme-linked immunosorbent assays and surface plasmon resonance analysis to assess their binding affinities to the target epitopes. (bvsalud.org)
More importantly, conventional assays rely on simple Ab-antigen binding assays, which may not result in identifying the most potent nAbs, as the strongest binder may not be the most potent nAb. (rsc.org)
Droplet microfluidic systems have the capability to overcome such limitations by conducting complex multi-step assays with high reliability, resolution, and throughput in a pico-liter volume water-in-oil emulsion droplet format. (rsc.org)

We show that immunization of HLA-A2 transgenic mice with tetramerized recombinant HLA-A2 incorporating HA-1 H minor histocompatibility antigen (mHag) peptides and β2- microglobulin (HA-1 H/HLA-A2) generate highly specific antibodies. (fujita-hu.ac.jp)
Immunization with AMA1 bound to RON2L, a 49-amino acid peptide from its ligand RON2, has shown superior protection by increasing the proportion of neutralizing antibodies. (bvsalud.org)
Interestingly, Fusion-FD12 immunization enhanced antibodies targeting conserved epitopes on AMA1, leading to increased neutralization of non-vaccine type parasites. (bvsalud.org)
Immunization studies showed that Fusion-F D12 immune sera neutralized parasites more efficiently than apoAMA1 immune sera despite having an overall lower anti-AMA1 titer, suggesting improvement in antibody quality. (bvsalud.org)
Furthermore, immunization with Fusion-F D12 enhanced antibodies targeting conserved epitopes on AMA1 resulting in greater neutralization of non-vaccine type parasites. (bvsalud.org)
Suppression was dependent on the ratio of haptenic groups on the molecule, appeared to be greatly affected by the method of immunization, and could be demonstrated in all three antigen systems. (caltech.edu)
The retention of antigen in rabbit liver tissue, resulting from a primary intravenous injection, is influenced by immunization brought about by subsequent intravenous injections of the same antigen. (caltech.edu)
Passively administered homologous antibody did not produce a change in the retention of the primary injection of antigen nor did secondary injections of a heterologous native protein injected according to the same immunization schedule as the homologous azoprotein. (caltech.edu)
Four monoclonal antibodies generated by immunization of mice with live T. foetus were selected on the basis of enzyme-linked immunosorbent assay reactions. (docksci.com)
Due to our unique combination of engineered mouse models, proprietary immunization techniques, and premium high-throughput and high-content screening capabilities, we specialize in high homology targets, constrained epitope targeting, and tough cell surface targets, all of which exist within the immuno-oncological antibody research space. (news-medical.net)

To allow the immune system to recognize millions of different antigens, the antigen-binding sites at both tips of the antibody come in an equally wide variety. (wikipedia.org)
The emergence of bispecific antibodies has been heralded as the fourth wave of biopharmaceutical innovation, providing opportunities to target two different antigens with a single therapeutic. (perkinelmer.com)
For example, some bispecific antibodies can bind different antigens expressed on two different cell types, bringing those cells together as a more effective treatment for complex diseases. (perkinelmer.com)

Bispecific antibodies (bsAbs) also present new challenges concerning process development and control, adds Thaddeus Webster, lead scientist, R&D Biologics for Lonza. (biopharminternational.com)

Virus-induced immune complex disease: identification of specific viral antigens and antibodies deposited in complexes during chronic lymphocytic choriomeningitis virus infection. (microbiologyresearch.org)
Using this binding mechanism, an antibody can tag a microbe or an infected cell for attack by other parts of the immune system, or can neutralize it directly (for example, by blocking a part of a virus that is essential for its invasion). (wikipedia.org)
The constant region at the trunk of the antibody includes sites involved in interactions with other components of the immune system. (wikipedia.org)
Antibodies from different classes also differ in where they are released in the body and at what stage of an immune response. (wikipedia.org)
Together with B and T cells , antibodies comprise the most important part of the adaptive immune system . (wikipedia.org)
For the past several years we have been interested in devising methods that would afford practical procedures for the isolation and purification of antibodies from immune serums and in particular the isolation of non-precipitating antibodies from animal serums and allergic antibodies (reagins) from human serums. (caltech.edu)
Most of the currently approved immuno-oncological therapeutics aim to enhance the immune response rather than directly target a specific tumor antigen. (news-medical.net)
Abveris is a contract research organization specializing in critical reagent and therapeutic antibody discovery for a wide range of targets, including those in the immune-oncology research space. (news-medical.net)
Our study indicates that co-expression of GP and VP40 antigens based on the SFV replicon vector generates EBOV VLPs in vitro , and vaccination with recombinant DREP vectors containing GP and VP40 antigens induces Ebola antigen-specific humoral and cellular immune responses in mice. (frontiersin.org)
In the following studies attributes of SAS1B are defined that support its candidacy like a tumor cell-specific target antigen, including tumor cell-surface convenience, immunogenicity, internalization of immune complexes into the endosomal-lysosomal system, and immunotoxin delivery resulting in tumor cell growth arrest = 4 experiments). (gasyblog.com)
Structures of Hepatitis B Virus Core- and e-Antigen Immune Complexes Suggest Multi-point Inhibition. (nih.gov)
Immune cells, meanwhile, suffer from large donor-to-donor variability, making both process characterization and development and the establishment of process control strategies difficult, particularly for autologous applications, according to Matuszczyk. (biopharminternational.com)
Although critical, antibodies are part of a larger and incompletely understood set of humoral and cellular immune responses, which has received little attention. (nature.com)
His research is aimed at understanding the cellular immune mechanisms responsible for the generation of IgG antibodies that recognize platelet antigens. (lu.se)
These antibodies can lead to clinically significant adverse effects such as autoimmunity causing immune thrombocytopenia (ITP) and alloimmunity causing platelet transfusion refractoriness. (lu.se)

One single-chain variable region moiety (scFv) antibody, #131, demonstrated high affinity (K D =14.9 nM) for the HA-1 H/HLA-A2 complex. (fujita-hu.ac.jp)
These data implicate that T cells with high-affinity antigen receptors reduce the ability to lyse targets with low-density peptide/MHC complexes (∼100 per cell), while they could respond at cytokine production level. (fujita-hu.ac.jp)
Antibodies play an ever increasing role in biotechnology and basic research sectors due to their ability to recognize their target antigen with high affinity and selectivity. (usi.ch)
This information helps to identify high-affinity antibody candidates that exhibit optimal target engagement and potential therapeutic efficacy. (iaanalysis.com)
Here, we describe a high-affinity minimal antibody, designated 5G7, obtained via a phage display screening against the C-terminal catalytic domain (ctd) of A3B. (uthscsa.edu)

Structural characterization of the fusion chimera, Fusion-F D12 to 1.55 Å resolution showed that it closely mimics the binary receptor-ligand complex. (bvsalud.org)
We are particularly interested in antibody D2.2, a potent neutralizer with the peculiarity of recognizing the toxin receptor binding domain and yet not inhibiting diphtheria binding to its host cellular receptor. (usi.ch)
The complex consists of several protein subunits (including gamma, delta, epsilon) associated with the T cell receptor (TCR). (histoprime.com)
The T cell receptor/CD3 complex: a dynamic protein ensemble. (histoprime.com)
Evidence for the T3-associated 90k heterodimer as the T cell antigen receptor. (histoprime.com)

The overall objective of EuroNeut-41 was to design a vaccine that raises antibodies able to prevent HIV cell fusion by blocking the virus fusion protein gp41 in its pre-fusogenic conformation. (europa.eu)
The utility and technical advantages of synthetic nucleic acid platforms (SNAP), including their efficacy, safety, speed of development, and ease of vaccine manufacture, were revealed during the COVID-19 pandemic, thus instilling hope that these technologies can be leveraged for the development of an effective HIV vaccine and broadly neutralizing antibody (bNAb) delivery. (nih.gov)
Dr. Terry Cyr , Health Canada, Ottawa, ON " Quantitative analysis of Influenza Vaccine Antigens: How Does the Hi3 Method Compare to Methods Requiring Labeled Isotopes? (cfabs.org)
However, this approach relies on the formation of a complex in solution between the two vaccine components. (bvsalud.org)
Identifying these cross-neutralizing antibody epitopes holds promise for developing an effective, strain-transcending malaria vaccine. (bvsalud.org)
A limitation of this approach, however, is that it requires the two vaccine components to form a complex in solution. (bvsalud.org)
Identifying epitopes of such cross-neutralizing antibodies will help in the development of an effective, strain-transcending malaria vaccine. (bvsalud.org)
Studying antibodies is also relevant for vaccine design, since most current vaccines aim to generate an antibody response. (usi.ch)
However, M2e-based vaccine candidates have shortcomings, including the need for several administrations and the lack of sustained antibody titers over time. (bvsalud.org)
We report here a vaccine targeting strategy that has the potential to confer sustained and strong protection upon a single shot of a small amount of M2e antigen. (bvsalud.org)
Identifying broadly conserved, protective antigens is important to vaccine development. (biomedcentral.com)

Mature messenger RNA species for both class I and class II major histocompatibility complex (MHC) molecules were detected by Northern hybridization, and their corresponding cell surface gene products were detected by cytofluorography of MCT cells stained with haplotype-specific antibodies. (rupress.org)
MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. (thermofisher.com)
Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. (thermofisher.com)
The CD3- molecules are involved in signal transduction after antigen binding to the TCR. (histoprime.com)
It quantifies the binding affinity between an antibody and its target antigen by tracking the migration of fluorescently labeled molecules.MST allows researchers to determine the dissociation constant (KD) with high sensitivity, even for low-affinity interactions. (iaanalysis.com)
The technology is also extensively used for kinetics measurements of antibodies and small molecules to assess the strength and speed of binding of an antibody to a target. (technologynetworks.com)
Also, measurements of nonspecific binding to compounds that are structurally related to the antigen of interest can help establish the likelihood of cross reactivity with other molecules that accompany the antigen. (technologynetworks.com)
ADC molecules have a complex structure with a number of different species [of molecule], each with different physical properties. (technologynetworks.com)

The most promising Ags were submitted to a more in-depth biophysical investigation of their structure and conformation stability, and for their capacity to interact with neutralising antibodies (Abs) and peptides. (europa.eu)
Dr. Eren's research focuses on understanding the structural and mechanistic aspects of Rev-host protein interactions and finding target regions to inhibit Rev activity using antibodies, synthetic peptides, and aptamers. (nih.gov)

Ion channel therapeutic monoclonal antibodies · 22. (synabs.be)
Our scientists work with you to apply the C1q binding assay method that has been qualified by commercially available therapeutic monoclonal antibodies to demonstrate performance, and can specifically verify or qualify your products. (party.biz)

The concept and methodology of protein microarrays was first introduced and illustrated in antibody microarrays (also referred to as antibody matrix) in 1983 in a scientific publication and a series of patents. (wikipedia.org)
Still, the RH antigens resisted many attempts to characterize their molecular basis: discussions continued whether all RH antigens derived from a single gene or whether there were three genes coding for C, D, or E. It was not until 1990 that the sequence of the Rh protein was elucidated [ 7,8 ]. (karger.com)
The CD9 antigen is also known as p24, tetraspanin-29 (Tspan-29), motility-related protein-1 (MRP-1), and leucocyte antigen MIC3. (bdbiosciences.com)
In this proposal, antibodies against Prion Protein and Diphtheria Toxin will be investigated. (usi.ch)
Recently, antibodies against the globular domain of prion protein have been shown to increase its toxicity. (usi.ch)
In a recent report by Garvey and Campbell (2) the loss of antigen material from hepatic tissue following a secondary injection of the native protein carrier was clearly demonstrated by radioautography. (caltech.edu)
1) The general approach has been to attempt to produce an insoluble protein antigen which would combine specifically with antibody to give a complex that could be dissociated into soluble antibody and insoluble antigen, which could then be separated by centrifugation. (caltech.edu)
Attempts were made to confer insolubility on protein antigens such as ovalbumin and crystalline bovine serum albumin without destroying their antigenicity. (caltech.edu)
However, in every case the resulting product either had lost too much native antigenicity (i.e., adsorbed serum always contained antibody which reacted with native antigen), was too soluble, or had too much power of adsorption for non-specific protein. (caltech.edu)
The problem of obtaining from precipitation experiments evidence about the structure of antibodies and the nature of serological reactions is obviously greatly simplified by the replacement of protein antigens by simple substances of known structure. (caltech.edu)
The molecular characterization of antibody binding to a superantigen-like protein from a commensal microbe. (cornell.edu)
Glycoprotein (GP) is the major protective antigen of EBOV, and can generate virus-like particles (VLPs) by co-expression with matrix protein (VP40). (frontiersin.org)
Although the assembled VLPs do not have S protein spikes, they could be developed as formulations that can improve the immunogenicity of vaccines including S antigens, and further could be used as platforms that can carry S antigens of concern for various mutations. (bvsalud.org)
Here we exploited a naïve human single chain antibody phage display library, to isolate binders against carefully chosen ORF3a recombinant epitopes located towards the extracellular N terminal and cytosolic C terminal domains of the protein using peptide antigens. (bvsalud.org)
Evidence indicates that Alix, an accessory protein of the endosomal sorting complex required for transport (ESCRT), is involved in the biogenesis of extracellular vesicles (EVs). (spandidos-publications.com)
Domain III (DIII) of the tick-borne encephalitis virus (TBEV) protein E contains epitopes, which induce antibodies capable of neutralizing the virus. (usf.edu)

We analyzed immunoglobulins (Ig) in explanted livers from SAH patients (n=45) undergoing liver transplantation and tissue from corresponding healthy donors (HD, n=10) and found massive deposition of IgG and IgA isotype antibodies associated with complement fragment C3d and C4d staining in ballooned hepatocytes in SAH livers. (elifesciences.org)
One antibody, TF1.15, also killed in the absence of complement. (docksci.com)
The radioimmunoassay of the group-specific antigen seems to be well suited to complement the COFAL (complement fixation avian leukosis) and RIF (resistance inducing factor) tests in the detection of leukosis virus in infected material. (microbiologyresearch.org)
Complement-fixing antigens in tissue cultures of avian leukosis viruses. (microbiologyresearch.org)
The complement system is a mechanism used by antibodies to eliminate pathogens and cancer cells in the body. (party.biz)
However, the activation of the complement cascade is strictly controlled and only occurs when the antibody binds to its antigen. (party.biz)
A diagnostic test based on sequencing long-lived SARS-CoV-2-specific memory T cells provides a complement to antibody testing for determining previous exposure to SARS-CoV-2. (nature.com)
The test is not intended for the diagnosis of active infection but is a complement to antibody tests used to confirm recent or previous infections. (nature.com)
These include additional antibody functions, such as antibody-dependent cellular cytotoxicity, complement activation and phagocyte recruitment. (nature.com)

Monoclonal antibodies to lymphocytic choriomeningitis virus and Pichinde viruses: generation, characterization and cross-reactivity with other arenaviruses. (microbiologyresearch.org)

Ig extracted from SAH livers, but not patient serum exhibited hepatocyte killing efficacy in an antibody-dependent cell-mediated cytotoxicity (ADCC) assay. (elifesciences.org)
Current therapy involves use of an "antitoxin" produced from horses known to cause "serum sickness", a problem that would be circumvented by treatment with human antibodies such as those studied in this project. (usi.ch)
The first was concerned with the rate and amount of excretion of soluble ^(35)S-labeled hemocyanin (KLH) and bovine serum albumin (BSA) following a single intravenous injection into normal rabbits and also with the properties of the excreted antigen material and its possible association with ribonucleic acid (RNA) or nucleotides. (caltech.edu)
Serum samples were then taken at various intervals and the specific activity of antibody was measured as soon as antibody reappeared in the circulation. (caltech.edu)
Quantitative antibody nitrogen data, obtained for the serum of each rabbit showed, in general, an inverse relationship between circulating antibody and radioactivity retained, i.e. the higher the circulating antibody titer, the lower the retention of radioactivity in liver tissue. (caltech.edu)
As a consequence, the identification of anti-GBM antibodies in the patient's serum or tissues is of paramount importance in the diagnosis of Goodpasture disease. (medscape.com)

First-generation immuno-oncological antibody therapeutics involved checkpoint modulators targeting common T-cell checkpoints such as PD-1 and CTLA-4. (news-medical.net)
Biologics, especially complex or multimodal therapeutics, are an area of continued innovation but are difficult to scale up, manufacture, store, and deliver for a variety of reasons," contends Don Owens, director of product development at TFF Pharmaceuticals. (biopharminternational.com)
Identifying antibodies (Abs) that neutralize infectious agents is the first step for developing therapeutics, vaccines, and diagnostic tools for these infectious agents. (rsc.org)

Since these functions are in vitro correlates of protection, the antigens recognized by these monoclonal antibodies may induce protective immunity. (docksci.com)
Identifying and characterizing conserved ETEC antigens that induce protective immunity is therefore of interest. (biomedcentral.com)

We used targeted mutagenesis of contact residues and affinity measurements and performed studies of the Fab-IbpA complex to determine the stoichiometry between Ibp and VH domains, suggesting Ibp may serve to cluster full-length IgA antibodies in vivo. (cornell.edu)
Competitive testing allows for the ranking and selection of antibodies based on their relative binding affinity. (iaanalysis.com)
Affinity measurements screen different isolates of an antibody to identify those that are most effective at binding antigen. (technologynetworks.com)

In particular ExoTEST is a successful platform for exosomes quantification and characterization from small amount of human biological fluids or cell media and it may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions. (immpact-international.org)
Even more precise targeting is on the horizon: for example, technologies that limit antibody activity to the tumor microenvironment and reduce cytotoxic activity in healthy tissue, resulting in a holistic attack on tumor cells with minimal adverse effects. (news-medical.net)
the endocytic pathway, and tumor cell growth arrest induced by antibody-toxin conjugates suggest therapeutic approaches that would selectively target tumors while limiting adverse drug effects in healthy cells. (gasyblog.com)
Multiple group specific antigen components of avian tumor viruses detected with chicken and hamster sera. (microbiologyresearch.org)
Radioimmunoassay for detecting group-specific avian RNA tumor virus antigens and antibodies. (microbiologyresearch.org)
Localization of avian tumor virus group-specific antigen in cell and virus. (microbiologyresearch.org)
examined the first medical usage of a fluorophore-labeled antibody focusing on carcinoembryonic antigen (CEA) for the recognition of colorectal tumor during medical procedures22. (p38-mapk-inhibitors.com)
In today's study, we describe the characterization and advancement of FITC-conjugated anti-CCSP-2 scFv, a book fluorescent probe for discovering colorectal tumor lesions by fluorescence colonoscopy. (p38-mapk-inhibitors.com)
The effect of bs-mAb-diDTPA-bs-mAb (DTPA is diethylenetriaminepentaacetic acid) bridging at the tumor cell surface on the internalization of the bs-mAb-diDTPA complex was investigated in SK-RC-52 tumor-bearing mice. (snmjournals.org)
Monoclonal antibodies (mAbs) directed against tumor-associated antigens have excellent characteristics to target tumor cells selectively ( 1 - 3 ). (snmjournals.org)

With every workshop on monoclonal antibodies to red blood cells [ 3,4,5,6 ], the number of D epitopes increased, and the D antigen seemed to become an extremely complex entity. (karger.com)
Proximal tubular epithelial cells from mice which develop autoimmune interstitial nephritis were found to express the nephritogenic target antigen, 3M-1. (rupress.org)
The 3M-1 antigen can be found on the MCT cell surface by radioimmunoassay, or deposited in a linear array in the extracellular matrix surrounding the MCT cells in culture by immunofluorescence. (rupress.org)
The CD9 antibody, clone M-L13, is derived from the hybridization of mouse myeloma cells with spleen cells isolated from mice immunized with common acute lymphoblastic leukemia (cALL) cells. (bdbiosciences.com)
I. Relationship of soluble antigens derived from infected BHK-21 cells to the structural components of the virion. (microbiologyresearch.org)
The study would benefit from a broader analysis of gut microbiota proteome and further characterization of B cells infiltrating the liver tissue including their numbers/field and their origin (infiltrating versus resident cells). (elifesciences.org)
After an antigen binds to a BCR, the B cell activates to proliferate and differentiate into either plasma cells , which secrete soluble antibodies with the same paratope, or memory B cells , which survive in the body to enable long-lasting immunity to the antigen. (wikipedia.org)
100-fold higher antigen density to exert cytotoxicity compared with the cognate CTL clone, they could produce inflammatory cytokines against cells expressing HLA-A2 and HA-1 H transgenes. (fujita-hu.ac.jp)
It should be pointed out that the radioautographic studies did not bear out the speculation presented by Campbell and Garvey (3, 4) that release of primary antigen resulted in uptake by adjacent hepatic cells and thus production of a "clone. (caltech.edu)
However it is possible that clones of cells might be formed in lymphoid tissues by such a mechanism of release and transfer of antigen. (caltech.edu)
These findings appear irreconcilable with the possibility of direct clonal selection of antibody-producing cells by unprocessed antigen. (caltech.edu)
The investigation was carried out in the following manner: rabbits were first immunized by a series of intravenous injections of antigen and at the height of precipitin production they were fed S35-labelled yeast cells. (caltech.edu)
The latter two monoclonal antibodies also mediated complementdependent killing of T. foetus and prevented of adherence of organisms to bovine vaginal epithelial cells. (docksci.com)
First, SAS1B is definitely exposed on the surface of uterine malignancy cells where it is accessible to antibody binding. (gasyblog.com)
Second, antibodies in the presence of match arrest the growth of SAS1Bpos uterine malignancy cells. (gasyblog.com)
The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage. (histoprime.com)
In this work we employed maleimide-thiol mediated conjugation of anti-CD4 F(ab') antibody fragments to enable nanoparticle targeted delivery of Eggmanone, a specific PDE4 inhibitor, to CD4+ T cells. (vanderbilt.edu)
Our novel delivery system targeted the majority of CD4+ T cells present among a complex cell population, and we have demonstrated antigen specific inhibition of CD4+ T cell responses mediated by nanoparticle formulated Egm. (vanderbilt.edu)
The Adaptive test involves laboratory-based next-generation sequencing to identify T cells that recognize SARS-CoV-2 antigens. (nature.com)
In ITP, for example, his laboratory is interested in how platelet antigens are processed and presented by antigen-presenting cells to activate T lymphocytes. (lu.se)
Functional characterization of natural killer cells in type I leukocyte adhesion deficiency. (lu.se)
SIV antigen has been demonstrated by immunohistochemical methods in lymph node sinus histiocytes, macrophages, and giant cells (14) as well as in macrophage-derived cells in brain tissue from diseased monkeys (8). (cdc.gov)
cells are activated by interaction with antigens and obtain T indicated that the cell lines representing a defined cell help, they mature into terminally differentiated plasma stage generally presented a high similarity in over- cells secreting large amounts of antibodies [10 -12]. (lu.se)

The class hence determines the function triggered by an antibody after binding to an antigen, in addition to some structural features. (wikipedia.org)
Structural analysis confirmed that the fusion chimera (Fusion-FD12) closely mimics the binary AMA1-RON2L complex. (bvsalud.org)
Despite decades of studies on Diphtheria toxin, no structural information is available on antibodies that can neutralize it. (usi.ch)
Structural characterization of the Myxococcus xanthus encapsulin and ferritin-like cargo system gives insight into its iron storage mechanism. (nih.gov)

The Expert Committee on Biological Standardization meets annually to establish detailed recommendations and guidelines for manufacturing, licensing, and control of complex biological materials, including blood products, vaccines, and related in vitro diagnostic tests, and to maintain a catalog of IRPs to be distributed globally as required. (cdc.gov)

Variants of Tacaribe virus resistant to neutralization by antibody 2.25.4 were obtained by growth in the presence of this antibody and neutralization kinetics were reexamined using the heterologous monoclonal neutralizing antibodies. (microbiologyresearch.org)

The NIAID study relied on a complex laboratory test to identify T-cell epitopes specific to SARS-CoV-2, employing a combination of mass cytometry and combinatorial staining of peptide-major histocompatibility complex (MHC)-bound tetramers. (nature.com)

This research addresses challenges in two distinct areas that are unified through the implementation of antibody conjugates that provide novel solutions for each challenge. (vanderbilt.edu)
In particular, antibody-drug conjugates (ADCs) are an exciting and emerging class of biopharmaceuticals with applications in cancer. (technologynetworks.com)
5 ADVANCES IN BLI BIOSENSORS Antibody-Drug Conjugates (ADCs) are an exciting and emerging class of biopharmaceuticals with applications in cancer. (technologynetworks.com)

The kinetics of reaction between antibody and virus were examined for all five neutralizing antibodies. (microbiologyresearch.org)
such as bio-layer interferometry (BLI) , allows real-time monitoring of antibody-antigen binding kinetics. (iaanalysis.com)
This eBook explores advances in biosensor technology and instrumentation that can significantly accelerate antibody discovery through efficient quantitation, kinetics and epitope binning. (technologynetworks.com)

While the impact of inactivated viral vaccines and live-attenuated bacterial vaccines on the host microbiota have been examined, the potential impact of using subunit vaccines consisting of antigens that are also encoded by commensal organisms has not been investigated. (biomedcentral.com)

His PhD investigated the complex molecular structure of gelatin-based hydrogels and was completed at the end of 2022. (edu.au)
Syroegin, E. A. , Aleksandrova, E. V. , and Polikanov, Y. S. (2022) Insights into the ribosome function from the structures of non-arrested ribosome-nascent chain complexes . (cornell.edu)

Structurally an antibody is also partitioned into two antigen-binding fragments (Fab), containing one V L , V H , C L , and C H 1 domain each, as well as the crystallisable fragment (Fc), forming the trunk of the Y shape. (wikipedia.org)
As scFv fragments, that may understand the same antigens as IgG antibodies, were created for fast focus on binding in molecular imaging25, the injection of scFv fragments allows penetration into tissue complexes as well as the rapid release and binding of antigens26. (p38-mapk-inhibitors.com)

[2] [3] Each tip of the "Y" of an antibody contains a paratope (analogous to a lock) that is specific for one particular epitope (analogous to a key) on an antigen, allowing these two structures to bind together with precision. (wikipedia.org)
The anti-GBM antibodies can target the EA and EB epitope separately. (medscape.com)
[ 4 ] The more abundant D-hexamers have dimer-reinforced crosslinks between NC1 domains that help to retain the cryptic nature of the Goodpasture epitopes, whereas the less abundant M-hexamers, composed of only monomeric subunits, allow epitope unmasking and antibody binding under inflammatory states. (medscape.com)
Both ORF3a N and C termini, epitope-specific monoclonal antibodies were identified in our study. (bvsalud.org)

Partial suppression was manifested in decreased frequency and delayed appearance of the response as well as decreased maximal antibody titers. (caltech.edu)

Monoclonal antibodies to murine hepatitis virus-4 (strain JHM) define the viral glycoprotein responsible for attachment and cell-cell fusion. (microbiologyresearch.org)
The pathogenesis of antibodies in severe alcoholic hepatitis (SAH) remains unknown. (elifesciences.org)
Capsids of hepatitis B virus e antigen with authentic C termini are stabilized by electrostatic interactions. (nih.gov)

Because these fluids were traditionally known as humors , antibody-mediated immunity is sometimes known as, or considered a part of, humoral immunity . (wikipedia.org)

Antibodies that detect MHC Class II (I-A/I-E) can be used in several scientific applications, including Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Functional Assay and Immunocytochemistry. (thermofisher.com)
Supernatants were tested for antibody by an enzyme-linked immunosorbent assay (ELISA), as described below, with whole T. foetus as the antigen. (docksci.com)
Until now, researchers have mostly relied on the use of lateral flow assay or enzyme-linked immunosorbent assay (ELISA) tests for SARS-CoV-2 antibodies to determine whether a person has been exposed to the virus. (nature.com)

[4] Soluble antibodies are released into the blood and tissue fluids , as well as many secretions . (wikipedia.org)
[5] The soluble Y-shaped units can occur individually as monomers , or in complexes of two to five units. (wikipedia.org)
The biological activity of soluble antigen-antibody complexes: II. (caltech.edu)
2), and ourselves (3) has established the fact that soluble antigen-antibody complexes formed in excess antigen can, (a) induce symptoms similar to anaphylaxis, (b) cause contraction of isolated smooth muscle from normal guinea pigs, and (c) increase the permeability of skin capillaries in a manner similar to that obtained in passive cutaneous anaphylaxis. (caltech.edu)

The project has been structured in 3 main pathways or "tracks": a Discovery track, aiming at designing gp41-based antigens (Ag) using an iterative improvement process, and two development tracks (Parenteral and Mucosal) to determine the safety and immunogenicity of the Ags and the optimal administration conditions before selecting the most promising candidate for clinical development. (europa.eu)
The results are discussed in terms of immunogenicity of sensitization, suppressive effects of persisting postsensitization antibody, and submolecular haptenic environment as factors possibly affecting the heterologous recall process. (caltech.edu)

Our MHC Class II (I-A/I-E) monoclonal and recombinant monoclonal antibodies are developed in Rat and Rabbit. (thermofisher.com)
While antibodies to AMA1 provide limited protection against Pf in non-human primate malaria models, clinical trials using recombinant AMA1 alone (apoAMA1) yielded no protection due to insufficient functional antibodies. (bvsalud.org)

While investigating the pathogenesis of experimental nephritis produced by rabbit anti-rat-kidney antibody, it occurred to us that the effects of antikidney antibody on kidney tissue might readily be visualized in tissue cultures. (caltech.edu)

This includes: antibody development, viral antigen characterisation, and the development of a membrane bound version of the molecular clamp. (edu.au)
Given the importance of A3B as a restriction factor of viral infections and as a driver of multiple human cancers, selective antibodies against A3B are highly desirable for its specific detection in various research and possibly diagnostic applications. (uthscsa.edu)
Here, we describe the development of PRESCIENT (Platform for the Rapid Evaluation of antibody SucCess using Integrated microfluidics ENabled Technology), a droplet microfluidic system that can enable high-throughput single-cell resolution identification of nAb repertoires elicited in response to viral infection. (rsc.org)

This project aims at investigating the interaction of antibodies with human pathogens, trying to improve our understanding of the molecular determinants for efficient neutralization. (usi.ch)

Clevers H, Dunlap S and Terhorst C. The transmembrane orientation of the epsilon chain of the TcR/CD3 complex. (histoprime.com)

Several different neutralization profiles were obtained, suggesting that point mutations resulted in conformational changes at topographically selected distinct epitopes recognized by the remaining antibodies. (microbiologyresearch.org)

By measuring the strength of antibody-antigen interactions, researchers can evaluate the efficacy of the antibody and its potential for therapeutic applications. (iaanalysis.com)
The ability to study complex interactions in solution makes MST a powerful tool for antibody characterization. (iaanalysis.com)
It provides real-time monitoring of biomolecular interactions, including antibody-antigen binding. (iaanalysis.com)

The major group-specific antigen (gs-a) of avian leukoviruses was detected by radioimmunoassay in extracellular medium of all embryonic cultures prepared from infected chickens. (microbiologyresearch.org)
Release of group-specific antigen into the extracellular medium was independent of subgroup or strain of avian leukosis or Rous sarcoma virus. (microbiologyresearch.org)

Characterization of a renal tubular epithelial cell line which secretes the autologous target antigen of autoimmune experimental interstitial nephritis. (rupress.org)
Anti-glomerular basement membrane (anti-GBM) antibody disease is a rare autoimmune disorder in which circulating antibodies are directed against an antigen normally present in the GBM and alveolar basement membrane. (medscape.com)
Pulmonary and/or renal manifestations can be encountered in various conditions, such as antineutrophilic cytoplasmic antibody (ANCA)-positive vasculitis and other autoimmune disorders. (medscape.com)
Two forms of cold antibody autoimmune hemolytic anemias are generally recognized: Donath-Landsteiner hemolytic anemia (DLHA) and cold agglutinin disease. (medscape.com)
[ 1 , 2 , 3 ] (Whipple et al reported a case of autoimmune hemolytic anemia in which paroxysmal cold hemoglobinuria was caused by an IgA Donath-Landsteiner [D-L] antibody. (medscape.com)

However, our group was able to show that single mutations in the antigen binding site render the molecule either toxic, non-toxic or protective, even when administered 20 days post prion infection. (usi.ch)
In this proposal we aim to determine and compare the molecular properties of toxic, non-toxic and protective antibody mutants. (usi.ch)

A limited antibody panel can distinguish B-precursor acute lymphoblastic leukemia from normal B precursors with four color flow cytometry: implications for residual disease detection. (bdbiosciences.com)
Evaluation of plaque size reduction as a method for the detection of Pichinde virus antibody. (microbiologyresearch.org)
The level may be too low for detection but still significant with respect to antibody formation. (caltech.edu)
Some studies have implicated L. tropica sults for the detection of anti- Leishmania Chang from Chicago University con- as another agent of VL in humans and antibodies(DAT,IFA,rK39dipstick) dogs in reports from the north-west and firmed the Leishmania identification were negative [ 2,10 ]. (who.int)

Isolation and characterization of CD34 + blast-derived exosomes in acute myeloid leukemia. (immpact-international.org)

The CD9 antibody recognizes a 24-kilodalton (kDa) cell surface glycoprotein belonging to the tetraspanin family. (bdbiosciences.com)
The antibody recognizes a unique molecule of the pathogen, called an antigen . (wikipedia.org)

Monoclonal antibodies prepared against Tacaribe and Junin viruses have been used to define further the serological relationships between arenaviruses of the Tacaribe complex. (microbiologyresearch.org)
Serological characterization. (microbiologyresearch.org)

The test emerged from a longstanding collaboration between Adaptive and Microsoft to apply machine learning to define the 'rules' according to which T-cell receptors (TCRs) identify their cognate antigens. (nature.com)
That, combined with extensive genomic characterization of the T-cell repertoires of people infected with SARS-CoV-2, allowed Adaptive to define a broad set of TCRs that are indicative of infection with the virus. (nature.com)

The CD3 complex is experimented on the cell membrane of T lymphocytes. (histoprime.com)
Beverley P C L and Callard R E. Distinctive functional characteristics of human T lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody. (histoprime.com)

Despite their efficiency, adoptive T-cell therapies show unique toxicities, which are distinct from those seen with conventional chemotherapies, monoclonal antibodies (mAbs), and small-molecule-targeted therapies. (nature.com)
The presence of dinitrophenyl groups on the immunizing antigen could suppress, partially or completely, the antibody response to p-azophenyl arsonate when this hapten was located on the same molecule. (caltech.edu)

Development and characterization of new monoclonal antibodies against porcine Interleukin-17A and Interferon-gamma. (usda.gov)
Molecular studies of LCM virus-induced immunopathology: development and characterization of monoclonal antibodies to LCM virus. (microbiologyresearch.org)
This information is crucial for selecting lead candidates for therapeutic development, understanding the mechanisms of action, and optimizing antibody design. (iaanalysis.com)
It provides valuable insights for antibody quality control and formulation development. (iaanalysis.com)
Our studies provide a platform for the development of selective antibodies against A3B and other APOBEC3 family enzymes. (uthscsa.edu)
The presented strategy and platform have the potential to identify and investigate neutralizing activities against a broad range of potential infectious agents for which nAbs have yet to be discovered, significantly advancing the nAb identification process as well as reinvigorating the field of Ab discovery, characterization, and development. (rsc.org)

Partial D phenotypes were found to lack specific epitopes defined by monoclonal antibodies [ 1 ], distinguishing them from weak D believed to express normal D antigen in reduced amounts. (karger.com)
ExoTEST consists of ELISA plates pre-coated with patented panexosome antibodies that allow specific capture of exosomes from a variety of biological samples, including cell culture supernatants and human biological fluids. (immpact-international.org)
If you cannot find the antibody you're looking for, contact us today to develop custom antibodies for specific targets, species and applications. (thermofisher.com)
The specific activity of antibody was measured in the antigen-antibody precipitates obtained, both in the period when the antibody had been allowed to decline and also after antigen had been reinjected to induce an anamnestic response. (caltech.edu)
A comparison of antibody-specific activity provided evidence that there was a release of antibody which had been made at the time of S35 feeding and stored in some stabilized form in tissues. (caltech.edu)
From these results it may be concluded that an intracellular antibody-forming activity influences the loss (or retention) of antigen deposited in liver tissue and that the mechanism is immunologically specific. (caltech.edu)
By competing with other antibodies for binding to the antigen, researchers can gain insights into the specific regions or amino acids on the antigen that are targeted by the antibody. (iaanalysis.com)
The N-terminal amino acid of an avian leukosis group-specific antigen from avian myeloblastosis virus. (microbiologyresearch.org)
Identification and localization of avian leukosis group-specific antigen within 'leukosis-free' chick embryos. (microbiologyresearch.org)
Origin of group-specific antigen of chicken leukosis viruses. (microbiologyresearch.org)
Immunofluorescent studies of group-specific antigen of the avian sarcoma-leukosis viruses. (microbiologyresearch.org)
Airway hyperresponsiveness, an exaggerated bronchospastic response to nonspecific agents such as methacholine and histamine or specific antigens, is the physiologic cornerstone of this disorder. (jci.org)
1999). For all diseases, and many chemicals have been implicated as caus- known respiratory allergens, specific IgE antibody has been ative agents (Cronin, 1980). (cdc.gov)

It was expected that the present study of excreted material would give further information as to the nature of retained antigen and also provide material for future investigation of the biological properties of the released material. (caltech.edu)
HCS platforms serve a multitude of purposes, from screening the effects of potential drug compounds on cellular processes to facilitating the study of complex biological pathways. (perkinelmer.com)
Biolayer Interferometry (BLI) is a label free technique that is widely used for the quantitation of antibodies, which is fundamental to biological research and production processes. (technologynetworks.com)
2017). BLI technology is widely used for quantitation of antibodies, which is fundamental to biological research and production processes. (technologynetworks.com)

The purpose of the present investigation was to study further, such antibody which appeared to be bound with antigen and perhaps normal tissue constituents. (caltech.edu)
In rabbits given a single primary intravenous injection of radioactive antigen, the retention of radioactivity in liver tissue, after a period of 21 days, was greater than when the primary injection was followed by secondary injections of the same, but non-radioactive antigen. (caltech.edu)
All of the radioactivity of material retained in the liver tissue 6 weeks after injection was immunologically related to the original S^(35)-BSA antigen. (caltech.edu)

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry. (thermofisher.com)

Thus in this proposal we aim to study the binding site and mechanism of action of a panel of human monoclonal antibodies. (usi.ch)
To understand the mechanism of A3Bctd recognition by 5G7, we used the crystal structures of A3Bctd and 5G7 as templates and computationally predicted the A3B-5G7 complex structure. (uthscsa.edu)

Anatomy21

Organisms17

Diseases5

Chemicals and Drugs138

Analytical, Diagnostic and Therapeutic Techniques and Equipment41

Phenomena and Processes36

Disciplines and Occupations2

Information Science3

Health Care4

Specificity14

Monoclonal antibody6

Proteins15

Chimeric11

Immunoglobulin7

Assays14

Immunization10

Different antigens3

Bispecific1

Immune15

High-affinity5

Receptor5

Vaccine11

Molecules8

Peptides2

Therapeutic monoclonal antibodies2

Protein16

Complement9

Cross-reactivity1

Serum6

Therapeutics3

Induce protective immunity2

Affinity3

Tumor10

Cells23

Structural4

Vitro1

Reexamined using the hetero1

Peptide1

Conjugates3

Kinetics3

Vaccines1

20222

Fragments2

Epitope4

Titers1

Hepatitis3

Immunity1

Assay3

Soluble4

Immunogenicity2

Recombinant2

Rabbit1

Viral3

Pathogens1

Transmembrane1

Epitopes recognized1

Interactions3

Extracellular2

Autoimmune5

Protective2

Detection4

Isolation1

Recognizes2

Serological2

TCRs2

Lymphocytes2

Molecule2

Development6

Specific13

Biological4

Tissue3

Identification1

Mechanism2