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Molecular characterizationSections using laser capChromosomeIsolationTumorGeneLaserCTCsFluorescenceDetectionMarkersStipulated use of laser capture microdiIsolation and CharacterizationCell CharacterizationProteomicPostmortem TissueTissueTranscriptomicMicroscopesAnalysesMolecular alterationsTissuesTumor samplesGenomicImmunohistochemistryTranscriptomeHeterogeneityProteinsLast decadeProteinClinicalConventionalAnalysisGene expressionDevelopmentSamplesAccurateCellsStudySampleResults

Molecular characterization2

• The bovine PPARGC1A gene: molecular characterization and association of an SNP with variation of milk fat synthesis. (fbn-dummerstorf.de)
• Single CTC from patients with lung cancer were used to demonstrate the potential of single CTC molecular characterization. (biomedcentral.com)

Sections using laser cap2

• Amyloid plaques and neighbouring non-plaque tissue were microdissected from human brain sections using laser capture microdissection and label-free LC-MS was used to quantify the proteins present. (researchsquare.com)
• White matter perivascular and intrameningeal immune infiltrates, including B cell follicle-like structures, were isolated from brain sections using laser capture microdissection. (biomedcentral.com)

Chromosome2

• Targeted generation of 16 sequence-tagged sites for bovine chromosome region 5q21-q25 by microdissection. (fbn-dummerstorf.de)
• Molecular and clinical characterization of Y chromosome microdeletions in infertile men: a 10-year experience in Italy. (medscape.com)

Isolation4

• Micro-sectioning coupled with laser capture microdissection (LCM) offers an opportunity for the isolation of feeding sites from heterogeneous cell populations. (biomedcentral.com)
• CTCs are present in the bloodstream at a very low concentration, thus their detection and characterization require highly sensitive and specific methods, which consist of a combination of enrichment (isolation) and detection (identification) strategies ( Table 1 ) and both steps are essential components of the identification process ( 19 ). (amegroups.org)
• One focus will be on exosome or micro-vesicles isolation, purification for bio-liquids, amount and size quantification, and characterisation techniques, including intra-cellular and membrane bound protein or lipid markers. (gene-quantification.de)
• Laser Capture Microdissection is a contact-free method of isolation & manipulation of live cells and fixed materials with precision. (unist.ac.kr)

Tumor1

• However, cancer cells are heterogeneous both within and between patients, forcing the need for individual characterization of the tumor cells. (biomedcentral.com)

Gene1

• Other examples of specimen requiring amplification for genome-wide characterization of gene expression include purified populations of cells obtained by either flow cytometry, laser capture microdissection, breast ductal or bronchial lavage, or microendoscopy. (biomedcentral.com)

Laser8

• Instead, we used laser microdissection to excise putative progenitor cells and control cells from their in situ locations in cryosections and characterized the molecular properties of these cells. (frontiersin.org)
• After detection, the male identity can be confirmed using Y-STR PCR after laser capture microdissection. (ugent.be)
• We collected 2000 individual cells per batch from FFPE liver slices with laser capture microdissection and used them as test samples. (bvsalud.org)
• The combination of MALDI mass spectrometry imaging, laser-capture microdissection, and quantitative proteomics allows the identification and characterization of molecularly distinct tissue compartments. (bvsalud.org)
• The two media that provided the best results (5% gelatin and 2% low-melting point agarose) were compared with non-embedded tissue by both MALDI mass spectrometry imaging of lipids and laser-capture microdissection followed by bottom-up proteomics. (bvsalud.org)
• 2% low-melting point agarose is proposed for tissue embedding in experiments involving MALDI mass spectrometry imaging of lipids and laser-capture microdissection, proteomics of consecutive tissue sections. (bvsalud.org)
• Using a workflow combining laser microdissection and single-cell mass spectrometry, the researchers identified more than 1,700 proteins per liver cell. (genomeweb.com)
• We use laser capture microdissection, next-generation sequencing, digital pathology, multiplexed immunohistochemistry and artificial intelligence to reach our goal. (labidigalylab.com)

CTCs2

• The implementation of molecular and genomic characterization of CTCs could contribute to improve the treatment selection and thus, to move toward more personalized treatments. (amegroups.org)
• We now can not only capture cancer cells from blood with high efficiency but also hand-pick single CTCs for in-depth characterization to provide crucial information that helps doctors make better decisions. (sciencedaily.com)

Fluorescence1

• Previous molecular characterizations of mammary stem cells (MaSC) have utilized fluorescence-activated cell sorting or in vitro cultivation of cells from enzymatically dissociated tissue to enrich for MaSC. (frontiersin.org)

Detection1

• Detection and further characterization of the male fetal cells in women post-partum with an autoimmune disease may result in a better understanding of the pathogenesis of the disease. (ugent.be)

Markers2

• Insights into the biology of stem cells will be gained by confirmation and characterization of candidate MaSC markers identified in this study. (frontiersin.org)
• Characterization of surface markers on extracellular vesicles isolated from lymphatic exudate from patients with breast cancer. (gu.se)

Stipulated use of laser capture microdi1

• The European and American methodological quality guidelines for microarray experiments were followed, including the stipulated use of laser capture microdissection for separation and purification of the lung cancer tumor cells from surrounding tissue. (upenn.edu)

Isolation and Characterization1

• Work conducted here includes both the isolation and characterization of adult tissue-derived stem cells and the generation of patient specific induced pluripotent cells (iPCs). (rosalindfranklin.edu)

Cell Characterization1

• Single-cell analysis has become an established method to study cell heterogeneity and for rare cell characterization. (labgenexp.eu)

Proteomic4

• Classification of amyloidosis by laser microdissection and mass spectrometry-based proteomic analysis in clinical biopsy specimens. (nih.gov)
• Extraction of venom and venom gland microdissections from spiders for proteomic and transcriptomic analyses. (nih.gov)
• In global proteomic and lipid studies without molecular amplification methods, the quantity of isolated cells sufficient to perform accurate characterization of less abundant species is problematic because the microscopic visualization, targeting, and isolation in LCM has a maximal rate of 1-20 cells per second, depending on the cells' microscopic distribution within the tissues. (nih.gov)
• We present a proteomic characterization wiring multiple sites in HNC, thus providing a promising basis for understanding tumoral biology and identifying metastasis-associated signatures. (uandes.cl)

Postmortem Tissue1

• Comprehensive histopathological characterization of postmortem tissue of a large sample of PD cases of different disease duration suggests that both the soma and the axon of mDA neurons is affected early after diagnosis, with loss of mDA neuron markers both in the SNc and in the striatum. (frontiersin.org)

Tissue7

• However, there have been few attempts to document heterogeneity in normal breast tissue with respect to proportion of stem, progenitor and mature cells at a given time and potential impact of this heterogeneity on tumor characterization, particularly for transcriptome analysis. (nature.com)
• Immunology services: Human monoclonal antibodies for therapy, breeding of transgenic and knockout mouse lines from common strain backgrounds and transfer onto preferred strain backgrounds (speed congenics), genotyping of transgenic and knockout mouse lines , tissue repository/tissue sharing/cryopreservation including the development and characterization of primary cell lines (for example, MEFs), experimental surgery services, health monitoring, monoclonal antibody production services. (nih.gov)
• To identify and collect spatially restricted samples in tissue, the center has a Leica Laser microdissection system and equipment for downstream analysis of gene expression by quantitative PCR. (rosalindfranklin.edu)
• If you are looking for a deep proteome characterization of tissue, Biogenity has tailored workflows for your research. (biogenity.com)
• Utilizing laser microdissection, Biogenity provides high-sensitivity proteomics with up to 3,000 proteins from 10^6 micron tissue pieces. (biogenity.com)
• We continue to develop our inventions of expression microdissection and laser capture microdissection for better integration with multiplex molecular analysis of specific cells and organelles extracted from complex tissue. (nih.gov)
• Laser capture microdissection (LCM) allows the capture of cell types or well-defined structures in tissue. (oncotarget.com)

Transcriptomic1

• This study combines rigorous early leaf characterization, laser capture microdissection, and transcriptomic sequencing to ask how patterns of gene expression regulate early leaf morphogenesis along the medio-lateral and proximal-distal axes in wild type Solanum lycopersium (tomato) and a leaf morphogenetic mutant trifoliate ( tf-2 ). (biorxiv.org)

Microscopes1

• Furthermore, application of laser capture microdissection microscopes has provided a rapid and reproducible approach to procure pure populations of cells. (nih.gov)

Analyses1

• Standard approaches such as in situ analyses and/or microdissection of different epithelial subpopulations and counting number of terminal duct lobular units have been used to document heterogeneity in the normal breast 5 . (nature.com)

Molecular alterations2

• However, characterization of molecular alterations occurring in C-MV of PTCs has so far been performed on whole tumor samples. (medscape.com)
• Characterization of the molecular alterations in premalignant lesions and the corresponding changes in the microenvironment would hasten the development of biomarkers for early detection and risk stratification as well as suggest preventive interventions to reverse or delay the development of cancer. (nih.gov)

Tissues3

• Immunoglobullin derived depositions in the nervous system: Novel mass spectrometry application for protein characterization in formalin-fixed tissues. (nih.gov)
• total protein) were compared across sample sets including cell lines, tissues subjected to laser capture microdissection, and blood-contaminated tissues. (roma2024.com)
• A fundamental argument for laser capture microdissection (LCM) of tissues is that, without separation of specific cell populations from complex tissues, we would miss critical control functions of thousands of regulated transcription factors, cell regulators, and receptors that are expressed at low copy number. (nih.gov)

Tumor samples1

• This body of work, recently published in Clinical Proteomics , advances the community's understanding of coring and LMD techniques in the context of integrated proteogenomic characterization of tumor samples. (cancer.gov)

Genomic2

• Morphologic and genomic characterization of urothelial to sarcomatoid transition in muscle-invasive bladder cancer. (tuni.fi)
• Editor's Note: Genomic and Molecular Characterization of Malignant Peripheral Nerve Sheath Tumor Identifies the IGF1R Pathway as a Primary Target for Treatment. (tuni.fi)

Immunohistochemistry1

• Immunohistochemistry and laser microdissection of patient renal biopsies will be used to assess in situ protein and RNA expression of innate interferons and their gene signatures. (rochester.edu)

Transcriptome1

• The atlas will integrate gene and protein expression profiles, transcriptome, epigenome, and other molecular characterizations with high-resolution anatomical information to provide molecular profiles of functionally or anatomically defined cell types in the developing lung. (nih.gov)

Heterogeneity1

• Heterogeneity in normal breast can have an influence on cancer stem cell (CSC) characterization. (nature.com)

Proteins2

• Functional characterization of venom proteins has important biomedical applications, including the identification of drug leads or probes for cellular receptors. (nih.gov)
• The venom collection procedure is followed by a protocol for dissecting spider venom glands, with results demonstrating that this leads to the characterization of venom-expressed proteins and peptides at the sequence level. (nih.gov)

Last decade1

• [ 4 ] In the last decade, laser capture microdissection (LCM) combined with mutational analysis has evolved as an important technical development for studying the clonality of individual components of heterogeneous lesions. (medscape.com)

Protein1

• Recent advances in mass spectrometry, especially platforms that permit rapid 'fingerprint' profiling of multiple biomarkers, and tandem mass spectrometers for protein identification, will most assuredly enhance the discovery, identification, and characterization of potential cancer associated biomarkers. (nih.gov)

Clinical2

• In addition, the TNF-transgenic mouse model has proven a valuable tool in the further understanding of the pulmonary vascular disease in scleroderma and with additional characterization, may provide a mechanism for the pre-clinical investigation of potential targets for treatment. (rochester.edu)
• The main research interest is within the frame of clinical genetic research, i.e. the characterization of acquired and inherited chromosomal aberrations with the aim to understand the biological background of how and why such alterations from the normal happen. (uniklinikum-jena.de)

Conventional1

• This presentation will provide fundamental descriptions of spatial applications of MS technology, including MALDI mass spectrometry imaging (MALDI-MSI) as well as the combined use of laser capture microdissection (LCM) with conventional mass spec analysis (LCMS) as an alternative method that provides identification and quantification of molecules with limited spatial context. (asip.org)

Analysis1

• If microdissection and molecular analysis can be made clinically practical, the expression levels of sets of approximately 20-100 critical, stage-specific disease markers within a selected cell population might provide reliable diagnosis and intermediate endpoints of response to molecular therapies in individual patients. (nih.gov)

Gene expression1

• To compare gene expression profiles of lacrimal gland duct and acinar cells after laser capture microdissection (LCM) and identify molecular networks related to K+ secretion, testing the hypothesis that duct cells are responsible for high K+ levels in tears. (nih.gov)

Development1

• A comprehensive characterization of PDAC is critical for the development of new therapies and the improvement of patient outcomes. (cancer.gov)

Samples1

• Here we isolated neuromelanin-positive dopamine neurons by laser capture microdissection from post-mortem human substantia nigra samples recovered at both early and advanced stages of PD. (frontiersin.org)

Accurate1

• This microdissection has allowed the accurate examination of both DNA content and RNA expression in prostate carcinoma. (nih.gov)

Cells2

• To identify the cellular sources of the expressed genes, cells from various layers of the retina were obtained using laser capture microdissection (LCM), and their mRNAs were isolated. (elsevierpure.com)
• Laser Capture Microdissection is a contact-free method of isolation & manipulation of live cells and fixed materials with precision. (unist.ac.kr)

Study1

• Objective: We aimed to evaluate RI study design, protocol differences, and accuracy of tumor diagnoses for their impact on carcinogenic hazard characterization. (nih.gov)

Sample1

• Data-driven characterization of molecular phenotypes across heterogeneous sample collections. (tuni.fi)

Results1

• I suggested to him that it would be interesting to adopt for the purpose the haemolytic streps associated with puerperal fever in view of the fact that the M.R. C was inaugurating an attack on this disease, and that therefore material and cooperation might be forthcoming and we might also hope to gather results which might be of use in characterization of strains. (nih.gov)