• Our results demonstrate that CcsZ is in fact an endo -acting cellulase belonging to glycoside hydrolase family 5 (GH-5). (biorxiv.org)
  • Cellulose degradation in Gastrophysa viridula (Coleoptera: Chrysomelidae): functional characterization of two CAZymes belonging to glycoside hydrolase family 45 reveals a novel enzymatic activity. (mpg.de)
  • The second ORF was the axhD3 gene belonging to glycoside hydrolase family 43. (megazyme.com)
  • Novel acid trehalase belonging to glycoside hydrolase family 37 from Pleurotus sp. (bvsalud.org)
  • We show that CcsZ is in fact capable of this activity and belongs to a broader family of glycoside hydrolases with unexpected taxonomic diversity. (biorxiv.org)
  • In this study, we found 61 families of glycoside hydrolases (GH) (out of 182 CAZymes) from protein sequences deposited in the CAZy database-currently associated with more than 20,324 microbial genomes. (biomedcentral.com)
  • The first ORF showed high homology with xylanases belonging to family 8 of the glycoside hydrolases and this gene was designated xylA . (megazyme.com)
  • The research focus is within the area "enzymes of extremophilic microorganisms", especially concerning thermostable glycoside hydrolases (including representatives from GH-family 1, 3, 10, 12 and 13). (lu.se)
  • 5. Rather, MY, Nordberg Karlsson E, Adlercreutz P. ( 2015 ) Complexation of alkyl glycosides with α-cyclodextrin can have drastically different effects on their conversion by glycoside hydrolases. (lu.se)
  • Purification, Characterization, and Stereochemical Analysis of a C-C Hydrolase: 2-Hydroxy-6-keto-nona-2,4-diene-1,9-Dioic Acid 5,6-Hydrolase. (warwick.ac.uk)
  • Octameric enolase from the hyperthermophilic bacterium Thermotoga maritima: purification, characterization, and image processing. (uni-regensburg.de)
  • Gamma-glutamyl hydrolase is an enzyme that catalyses the following chemical reaction: Hydrolysis of a gamma-glutamyl bond This lysosomal or secreted, thiol-dependent peptidase, most active at acidic pH. (wikipedia.org)
  • In humans, gamma-glutamyl hydrolase is encoded by the GGH gene. (wikipedia.org)
  • Three-dimensional structure of human gamma -glutamyl hydrolase. (wikipedia.org)
  • gamma-Glutamyl hydrolase ( GGH, EC 3.4.19.9) catalyzes removal of the polyglutamyl tail from folyl and p-aminobenzoyl polyglutamates. (univpm.it)
  • To test this hypothesis, we subcloned the putative glycoside hydrolase CcsZ and solved the X-ray crystal structure of both apo- and product-bound CcsZ. (biorxiv.org)
  • 2]Wang X C, Xue L, Sun J Q, Zuo J R. The Arabidopsis BE1 gene, encoding a putative glycoside hydrolase localized in plastids, plays crucial roles during embryogenesis and carbohydrate metabolism. (chinacrops.org)
  • 8. von Schantz L, Schagerlöf H, Nordberg-Karlsson E, Ohlin M. ( 2014 ) Characterization of the substitution pattern of cellulose derivatives using carbohydrate-binding modules. (lu.se)
  • Advances in understanding the molecular and biochemical basis include cloning and characterization of most genes that encode specific enzymes required for sphingolipid metabolism. (medscape.com)
  • The diverse ionization behaviors of the histidine residues are discussed in terms of their structural and functional roles in this model glycoside hydrolase. (ubc.ca)
  • however, in addition to organic synthesis and med chem optimization our lab also pursues assay development, high-throughput screening, biochemical/biophysical ligand-receptor analysis, structural characterization, and computational modeling. (purdue.edu)
  • Characterization of a family 43 β-xylosidase from the xylooligosaccharide utilizing putative probiotic Weissella strain 92. (lu.se)
  • A concordant reduction (22 - 33 %) in TH, alpha-synuclein (SNCA), and ubiquitin C-terminal hydrolase L1 (UCHL1 / PARK5) proteins was also seen in the STR, suggesting that a short-term repeated exposure to WF causes dopaminergic neurotoxicity. (cdc.gov)
  • Cloning and characterization of arabinoxylan arabinofuranohydrolase-D 3 (AXHd 3 ) from Bifidobacterium adolescentis DSM2008 3 . (megazyme.com)
  • cloning, expression and characterization. (bvsalud.org)
  • It is ranging from cloning and molecular development to production and characterization. (lu.se)
  • Biochemical characterization and homology modeling of a purine-specific ribonucleoside hydrolase from the archaeon Sulfolobus solfataricus: Insights into mechanisms of protein stabilization. (cnr.it)
  • Several substrates were employed in the biochemical characterization of recombinant AXHd3. (megazyme.com)
  • Functional Characterization of the Glycoside Hydrolase Encoding Gene OsBE1 during Chloroplast Development in Oryza sativa [J].Acta Agron Sin, 2014, 40(12): 2090-2097. (chinacrops.org)
  • Evolution and functional characterization of CAZymes belonging to subfamily 10 of glycoside hydrolase family 5 (GH5_10) in two species of phytophagous beetles. (mpg.de)
  • Functional characterization of poplar and Arabidopsis wood - related hydrolases by reverse genetics and overexpression in Arabidopsis : effects on hypocotyl elongation. (slu.se)
  • The started in 1953 in the north-east of the smears were fixed in methanol, stained by country [ 8 ] but, while it was extended to standard Giemsa methods and examined other parts of the country, the isolation and for parasites by light microscope at high characterization of the parasites has not magnification (× 1000). (who.int)
  • This work reports the characterization of the first known peptidoglycan hydrolase (Acp) produced mainly during vegetative growth of Clostridium perfringens. (pasteur.fr)
  • 98th percentile) transcripts, including acetone carboxylase and cell-wall-associated hydrolases with unknown substrates (numerous lesser expressed cell-wall-associated hydrolases targeted peptidoglycan). (frontiersin.org)
  • 3]Wang X C, Yang Z R, Wang M, Meng L Z, Jiang Y W, Han Y H. The BRANCHING ENZYME1 gene, encoding a glycoside hydrolase family 13 protein, is required for in vitro plant regeneration in Arabidopsis. (chinacrops.org)
  • In vitro characterization revealed that the strains tolerate gastric and bile challenges and display a great adhesion capacity to human intestinal cells. (frontiersin.org)
  • Our lab is currently pursuing best-in-class inhibitors versus Ubiquitin C-terminal hydrolase L1 (UCHL1). (purdue.edu)
  • Pig lactase/phlorizin hydrolase was shown to have the same quaternary structure as the human enzyme, i.e., built up of two polypeptides of the same molecular weight (160000). (nih.gov)
  • Antiserum to the purified enzyme raised in rabbits cross-reacted with rat and Guinea pig epoxide hydrolases. (elsevierpure.com)
  • Characterization of a Nitro-Forming Enzyme Involved in Fosfazinomycin Biosynthesis. (acs.org)
  • Pig intestinal lactase/phlorizin hydrolase (EC 3.2.1.23/62) was purified in its amphiphilic form by immunoadsorbent chromatography. (nih.gov)
  • In each disorder, a deficiency of a lysosomal hydrolase is inherited, which leads to lysosomal accumulation of the enzyme's specific sphingolipid substrate. (medscape.com)
  • 2016) Construction of the first thermostable β-glucosynthase derived from glycoside hydrolase family 3. (lu.se)
  • The lactase/phlorizin hydrolase isolated from pigs in which the pancreatic ducts had been disconnected 3 days before death and from Ca2+-precipitated enterocyte membranes (basolateral and intracellular membranes) exhibited in SDS-polyacrylamide gel electrophoresis the same size of constituent polypeptides and the same catalytic and immunological properties as a normal brush border lactase/phlorizin hydrolase. (nih.gov)
  • Pteroylpolyglutamate hydrolase from human jejunal brush borders. (wikipedia.org)
  • Pre-Steady-State Kinetic Analysis of 2-Hydroxy-6-keto-nona-2,4-diene-1,9-dioic Acid 5,6-Hydrolase: Kinetic Evidence for Enol/Keto Tautomerization. (warwick.ac.uk)
  • Characterisation and cytomodulatory properties of peptides from Mozzarella di Bufala Campana cheese whey. (cnr.it)
  • A comprehensive analysis of the PG biosynthesis machinery in H. neptunium shows that the conserved actin homologue MreB, the PG synthases PBP2 and PBP3, and the PG hydrolase LmdC play a vital role in cell growth in H. neptunium. (uni-marburg.de)
  • This is completely different from the parasitic protozoa IU-NH, specifically the nucleoside hydrolase from C. fasciculata, which showed no activity toward deoxynucleosides. (mtsu.edu)
  • HepG2 cells and medium were assayed for cholesteryl ester hydrolase (CEH) activity in the presence and absence of sodium cholate. (psu.edu)
  • Systems that previously were considered too complex for a molecular characterization now successively become available to fundamental studies. (lu.se)