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  • ligands
  • These and other data indicate that His412, Asp414 and His483 provide the three ligands required for the binding of Fe 2+ to a catalytic site, while Lys493 provides the residue required for binding of the C‐5 carboxyl group of 2‐oxoglutarate. (embopress.org)
  • Subsequent interpretation and mutagenesis studies provide insight into the binding of this tetratricopeptide repeat-like domain to mitotic checkpoint-relevant protein ligands. (bl.uk)
  • kainate
  • Dechesne C. J., Hampson D. R., Goping G., Wheaton K. D., and Wenthold R. J. (1991) Identification and localization of a kainate binding protein in the frog inner ear by electron microscopy immunocytochemistry. (springer.com)
  • Substrate
  • A catalytic site appears to comprise a set of separate locations for the binding of the various co‐substrates and the peptide substrate. (embopress.org)
  • mGluR4
  • 4 l‐CCG‐I evoked an inhibition of the forskolin‐stimulated cyclic AMP production characteristic of mGluR4 with a potency comparable to l‐glutamate. (deepdyve.com)
  • therapeutic
  • Many of these flexible enzymes are of biological interest, and hindering their function via binding of competitive inhibitors would open up valuable therapeutic avenues. (illinois.edu)
  • inhibitors
  • Structure-based modelling suggested the binding modes of the inhibitors in the active site and allowed an estimation of the possible contacts formed upon binding. (pubmedcentralcanada.ca)
  • Inhibitors of glutamate racemase could act as novel antibiotics against a target to which there are no current antibiotics, and thus no known resistance. (illinois.edu)
  • Spies, M. A. Exploiting enzyme plasticity in virtual screening: high efficiency inhibitors of glutamate racemase. (illinois.edu)
  • Spies, M. A. In silico optimization of a fragment-based hit yields biologically active, high-efficiency inhibitors for glutamate racemase. (illinois.edu)
  • protein
  • The deduced PTOX protein encoded by both paralogs were proposed to be functional, since the existence of highly conserved amino acid sites necessary for a typical PTOX, including six iron-binding sites and Exon 8 domain. (thefreelibrary.com)
  • molecular
  • At birth, these binding sites were diffused through the whole cerebellar mass, but became distinctly concentrated in the molecular and the internal granular layers by postnatal day 10. (muscimol.xyz)
  • In the molecular layer the Cl-/Ca2+ effect increased during ontogeny until, in adults, the ion-dependent binding was threefold higher than the ion-independent binding. (muscimol.xyz)
  • Starting from postnatal day 15, binding site density in the molecular layer of lobules VIb and VII of the vermis was lower than in other lobules. (muscimol.xyz)
  • The increase of the Cl-/Ca(2+)-dependent binding in the molecular layer is simultaneous with the growth of Purkinje cell dendrites and of parallel fibres and with the formation of the synapses between them. (muscimol.xyz)
  • The low density of [3H]L-glutamate binding in the molecular layer of lobules VIb and VII probably indicates the presence of specific nerve projections to these areas. (muscimol.xyz)
  • The results provide a molecular basis for the tight coupling of agonist binding and receptor activation. (aspetjournals.org)
  • enzyme
  • The application of isothermal titration calorimetry in combination with enzyme kinetics showed that three designed pyrimidine derivatives bind to lumazine synthase with micromolar dissociation constants and competitively inhibit the catalytic reaction. (pubmedcentralcanada.ca)
  • The following work focuses on one particular enzyme, glutamate racemase. (illinois.edu)
  • Glutamate racemase is an essential and non-redundant enzyme in all species of bacteria, and inhibition of this enzyme results in cell wall degradation, followed by imminent cell death. (illinois.edu)
  • amino
  • Quisqualate-sensitive sites accounted for 80% of the total binding sites already at postnatal day 15, while displacement by alpha-amino-3-hydroxy-methyl-4-isoxazolepropionic and ibotenic acids attained the maximum (68%) at postnatal day 60. (muscimol.xyz)
  • predict
  • The results of the second two projects then feed back into our in silico methods in order to improve our capacity to predict small molecule binders of glutamate racemase. (illinois.edu)