• When genetically susceptible individuals are exposed to infection, stress or trauma, cutaneous dendritic cells are activated to produce tumor necrosis factor (TNF)-α and interleukin (IL)-23, which subsequently stimulate the proliferation and differentiation of proinflammatory T cells. (nature.com)
  • Both enriched and clonal populations of primary myoblasts were characterized in assays of cell proliferation and differentiation. (rupress.org)
  • A mitogen receptor complex which influences reactions leading to proliferation and differentiation. (uni-mainz.de)
  • However, the possible differences in proliferation and differentiation capabilities among independently-derived human embryonic stem cells (hESCs) are not well known because of insufficient characterization. (biomedcentral.com)
  • Development and homeostasis require the coordinate regulation of cell proliferation and differentiation. (biomedcentral.com)
  • Stem cells and progenitor cells have this ability in common. (wikipedia.org)
  • However, stem cells are less specified than progenitor cells. (wikipedia.org)
  • Progenitor cells can only differentiate into their "target" cell type. (wikipedia.org)
  • The most important difference between stem cells and progenitor cells is that stem cells can replicate indefinitely, whereas progenitor cells can divide only a limited number of times. (wikipedia.org)
  • Some research found that progenitor cells were mobile and that these progenitor cells could move through the body and migrate towards the tissue where they are needed. (wikipedia.org)
  • Many properties are shared by adult stem cells and progenitor cells. (wikipedia.org)
  • Progenitor cells have become a hub for research on a few different fronts. (wikipedia.org)
  • Current research on progenitor cells focuses on two different applications: regenerative medicine and cancer biology. (wikipedia.org)
  • Research on regenerative medicine has focused on progenitor cells, and stem cells, because their cellular senescence contributes largely to the process of aging. (wikipedia.org)
  • Research on cancer biology focuses on the impact of progenitor cells on cancer responses, and the way that these cells tie into the immune response. (wikipedia.org)
  • Increased circulation of progenitor cells in the body has also positively correlated to increased longevity and regenerative processes. (wikipedia.org)
  • Endothelial progenitor cells (EPCs) are one of the main focuses of this field. (wikipedia.org)
  • Recent studies have shown that haematopoietic progenitor cells contribute to immune responses in the body. (wikipedia.org)
  • Inflammatory cytokines and other factors released during infections will activate haematopoietic progenitor cells to differentiate to replenish the lost resources. (wikipedia.org)
  • The characterization or the defining principle of progenitor cells, in order to separate them from others, is based on the different cell markers rather than their morphological appearance. (wikipedia.org)
  • Intermediate progenitor cells formed in the subventricular zone. (wikipedia.org)
  • These progenitor cells are easily identified by their long radial process. (wikipedia.org)
  • Bone marrow stromal cells found in the epidermis and make up 10% of progenitor cells. (wikipedia.org)
  • Periosteum contains progenitor cells that develop into osteoblasts and chondroblasts. (wikipedia.org)
  • Pancreatic progenitor cells are among the most studied progenitors. (wikipedia.org)
  • Angioblasts or endothelial progenitor cells (EPC). (wikipedia.org)
  • after that period, progenitor cells produce only dissimilar mesenchymal stem cell daughters. (wikipedia.org)
  • The retinal progenitor cells (RPCs) of retinal organoids expressed CD184, consistent with its expression in the neuroblastic layer in fetal retina. (caltech.edu)
  • The team showed that IER3IP1-- mutant organoids are abnormally small because their neural progenitor cells differentiate into neurons before the organoids properly expand. (the-scientist.com)
  • In vitro differentiation of GS-2 iPSCs into hematopoietic stem and progenitor cells was done using Op9 feeder layers and specified media. (biomedcentral.com)
  • derivation of this specialized endothelium Ezetimibe enzyme inhibitor from human being embryonic stem cells (hESCs) provides an priceless platform to study and dissect blood specification and the emergence of hematopoietic stem and progenitor cells. (bioskinrevive.com)
  • We have developed and established protocols for human iPSC neurons on microelectrode arrays, MEAs, together with our commercial partners who offer iPSC-neurons and neuronal progenitor cells, NPC's. (neuroproof.com)
  • Human CD34 + hematopoietic stem and progenitor cells purified from umbilical cord or adult peripheral blood were cultured in a two-phase liquid culture system in a serum-free medium for 21 days. (haematologica.org)
  • We found that INK4b and INK4a , but not ARF , are upregulated following the differentiation of haematopoietic progenitor cells, in ageing fibroblasts and in senescing malignant rhabdoid tumour cells. (biomedcentral.com)
  • Downloaded from periodic thymic colonization by progenitor cells guarantees stage. (lu.se)
  • The focus of my research is to understand cell fate specification in the developing brain and in human neural progenitor cells using cell-based models of neuronal differentiation. (lu.se)
  • In vitro differentiation of Th9 cells from the larger CD4 + T cell population provides increased numbers of Th9 cells to facilitate research into their functions. (rndsystems.com)
  • In vitro differentiation of small intestinal epithelium that approaches physiologic functionality of the in vivo host may allow for the development of a pathogenesis model for norovirus. (cdc.gov)
  • Development of Antiviral Innate Immunity During In Vitro Differentiation of Mouse Embryonic Stem Cells. (usm.edu)
  • Based on its restriction in expression and its modulation during in vitro differentiation, it is likely that matk participates in signal transduction during megakaryocytopoiesis. (ox.ac.uk)
  • Second, altering the MSC state by inducing differentiation into multiple lineages did not affect the exosomal properties or endocytosis but triggered the expression of lineage-specific genes and proteins in vitro and in vivo respectively. (frontiersin.org)
  • What makes characterization challenging is that heterogeneity within a given stem cell population can cause variability in the differentiation into important downstream lineages, such as cardiomyocytes and hepatocytes. (genengnews.com)
  • Most importantly stem cells possess the ability to differentiate into cell types of multiple lineages including adipocytes, chrondrocytes, and osteocytes. (vin.com)
  • MSCs are a population of nonhematopoietic adult stem cells that have the property of self-renewal and can differentiate into multiple lineages [ 8 - 10 ]. (hindawi.com)
  • A unique and essential property of embryonic stem cells is the ability to self-renew and differentiate into multiple cell lineages. (biomedcentral.com)
  • All cell lines differentiated into three embryonic germ lineages in embryoid bodies and into neural cell lineages when cultured in neural differentiation medium. (biomedcentral.com)
  • By using this technology we show that during the course of differentiation of human myeloid precursor cells into three different lineages, the cells alter their viscoelastic properties, to suit their ultimate fate and function. (exeter.ac.uk)
  • First, it was tested whether patient‑derived ADSCs could differentiate into chondrogenic and osteogenic lineages. (spandidos-publications.com)
  • Exosomes are a non-cellular resource and therefore free from the potential to differentiate into unintended cellular lineages, which is a major problem of MSC transplantation [ 18 ]. (biomedcentral.com)
  • Following cell differentiation, flow cytometry can be used to verify the expression of established cell surface or intracellular markers of CD4 + T cell subsets. (rndsystems.com)
  • Determine the percentage of differentiated cells by flow cytometry using the suggested reagents shown in the table below. (rndsystems.com)
  • To prepare cells for flow cytometry, wash the cells once with RPMI and resuspend them in 1 mL of RPMI, 2 mM L-glutamine, 10 units/mL penicillin, 10 µg/mL streptomycin, 10% FBS, 50 ng/mL PMA, and 1 µg/mL calcium ionomycin. (rndsystems.com)
  • For protocols to stain membrane-associated molecules or intracellular molecules (using either detergents or alcohol to permeabilize the cell membrane), please visit Flow Cytometry Protocols on our website. (rndsystems.com)
  • Additionally cell surface marker characterization via flow cytometry differentiates them from hematopoetic cells, though no truly unique MSC molecule has been identified. (vin.com)
  • The overall goal of this study is to compare two different methods of human monocyte isolation for dendritic cell generation in vitro and to characterize the CT11c, CT14 cell surface expression of the resulting monocyte derived populations by imaging flow cytometry. (jove.com)
  • In addition, this is the first study to characterize the specific population of monocyte derived dendritic cells by single cell imaging flow cytometry. (jove.com)
  • D) Representative flow cytometry plots of cells stained with fluorescein isothiocyanate (FITC)-conjugated anti-CD71 and phycoerythrin (PE)-conjugated anti-CD235a antibodies, demonstrating sequential expression of CD71 followed by CD235a and subsequent loss of CD71. (haematologica.org)
  • Using immunocytochemistry, flow cytometry, qRT-PCR and MPSS, we found that all three cell lines actively proliferated and expressed similar "stemness" markers including transcription factors POU5F1/Oct3/4 and NANOG, glycolipids SSEA4 and TRA-1-81, and alkaline phosphatase activity. (biomedcentral.com)
  • During differentiation, morphology and the relative expression of sox-17, pdx-1, Ins-1 and Ins-2 genes were assessed, also the production of insulin and glucagon proteins was determinated by fluorescence microscopy and flow cytometry. (intjmorphol.com)
  • Phenotypical change of non-parenchymal liver cells was also evaluated by flow cytometry. (biomedcentral.com)
  • The most widely accepted method for determining whether a population of cells is truly pluripotent is the teratoma assay, that is, verifying in vivo a cell population's ability to differentiate into each of the three germ layers via teratoma formation in mice. (genengnews.com)
  • Primary myoblasts were dependent on added bFGF for growth and retained the ability to differentiate even after 30 population doublings. (rupress.org)
  • Recently, a number of studies have confirmed that ADSCs possess the ability to differentiate into adipocytes, osteoblasts, and chondrocytes [ 16 - 18 ], suggesting that a broader source of stem cells is available for application in tissue engineering. (hindawi.com)
  • Human embryonic stem cells (hESCs) possess the ability to self-renew in an undifferentiated state in culture while retaining the ability to differentiate into all of the cell types in the human body. (biomedcentral.com)
  • His current research projects utilize mouse embryonic stem cells as a model system to investigate the molecular mechanisms that control stem cell differentiation and the development of innate immunity. (usm.edu)
  • The Molecular Basis for the Lack of Inflammatory Responses in Mouse Embryonic Stem Cells and Their Differentiated Cells. (usm.edu)
  • Mouse embryonic stem cells are deficient in type I interferon expression in response to viral infections and double-stranded RNA. (usm.edu)
  • Due to the boom in regenerative medicine, Stem Cells (SC) represent a source of cell replacement to any tissue, we decided to undertake this research with the objective of differentiating mouse embryonic stem cells (mESC) to early pancreatic cells, developing their genetic and morphological characterization. (intjmorphol.com)
  • Boundary cap neural crest stem cells promote survival of mutant SOD1 motor neurons. (wikipedia.org)
  • The differential expression timing of CD184 and CD171 permits identification and enrichment of RGCs from retinal organoids at differing maturation states from committed progenitors to differentiating neurons. (caltech.edu)
  • To understand the differentiation process of these stem cell cultures into neurons is an important and complex task and an essential prerequisite for the functional characterization of test compounds in a human cellular background.These iPSC cultures can be differentiated to different neuron cell type cultures as glutamatergic, gabaergic, dopaminergic or motoneurons and others for further information see also on our stem cells technology page. (neuroproof.com)
  • Deconvolution shows dopamine neurons being the dominating cell type in many features beneath the graft area. (lu.se)
  • Our current focus is to learn how to direct and efficiently drive controlled differentiation of human stem cells into subtype-specific neurons. (lu.se)
  • Although MSC EVs show great promise for regenerative medicine applications, insights into their uptake mechanisms by different target cells and the ability to control MSC EV properties for defined function in vivo have remained elusive knowledge gaps. (frontiersin.org)
  • Mesenchymal stem cells clearly modulate immune responses, as demonstrated by both in vitro and in vivo studies. (vin.com)
  • Among these, adipose-derived stem cells (ADSCs) are a type of mesenchymal stem cell isolated from adipose tissue, which has the advantages of abundant storage in vivo , easy acquisition, and expansion [ 13 - 15 ]. (hindawi.com)
  • In vivo differentiation to cells from all three germlines was tested using a teratoma assay. (biomedcentral.com)
  • Cells grown on porous collage-coated beads under fluid shear conditions in rotating wall vessel bioreactors differentiate into 3-D architectures resembling both the morphologic and physiologic function of in vivo tissues. (cdc.gov)
  • We first optimized a serum-free, miniature erythroid differentiation system starting from primary human CD34 cells, the exact type of cells we would ultimately like to target in vivo ( Figure 1 ). (haematologica.org)
  • Immortalized cells were clonally expanded and characterized based on collagen type I, collagen type II, α1 (COL2A1), and SRY-box 9 (SOX9) protein expression profiles, as well as on expression of a subset of established in vivo NP cell lineage markers. (biomedcentral.com)
  • Most clinical trials which utilize ADSCs for OA treatment have been based on the autologous cells from the stromal vascular fraction, as have most of the in vivo studies ( 6 , 7 ). (spandidos-publications.com)
  • Mouse nuclear transfer embryonic stem cells (NT-ESCs) were first established in 2000, and then proved to be able to differentiate either in vivo or in vitro, and give rise to individual tissues through germ line transmission or tetraploid complementation. (benthamscience.com)
  • 2004): Subcutaneous adipocytes can differentiate into bone- forming cells in vitro and in vivo. (ankara.edu.tr)
  • Deconvolution of spatial sequencing provides accurate characterization of hESC-derived DA transplants in vivo. (lu.se)
  • Despite increasing refinement of differentiation protocols and standardization of the transplanted neural precursors, the transcriptomic analysis of cells in the transplant after its full maturation in vivo has not been thoroughly investigated. (lu.se)
  • 5. Gronthos S, Mankani M, Brahim J, Robey PG, Shi S. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. (bvsalud.org)
  • Scholars@Duke publication: Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress. (duke.edu)
  • Human iPSC derived neuronal cell cultures cultivated on microelectrode arrays. (neuroproof.com)
  • Primary neuronal cell cultures from mouse and iPSC derived neuronal cultures show similar activity development over time. (neuroproof.com)
  • The expression of cardiac markers in iPSC-derived cardiomyocytes and the electrical properties of the cells. (creative-bioarray.com)
  • We characterize the role of increased Lyn kinase activity in neutrophils, endothelial cells and lesional liver biopsies and utilized an iPSC-derived endothelial cell platform for disease modeling of neutrophilic vasculitis and to screen and evaluate drug efficacy. (nature.com)
  • Further transcriptomic analysis of isolated peripheral blood mononuclear cells (PBMCs) and PMNs from patients with acute brucellosis before treatment initiation and after successful treatment revealed a positive correlation of the molecular signature of active disease with pathways associated with response to interferons (IFN). (frontiersin.org)
  • These observations will facilitate molecular characterization of PSC-derived RGCs during differentiation, critical knowledge for establishing the veracity of these in vitro produced cells. (caltech.edu)
  • We explored the underlying molecular mechanisms for ectodermal differentiation in our model and observed up-regulation of β-catenin, noggin, VCAN, and SMAD genes. (ku.edu)
  • The aim of this study was to test for the molecular and functional expression of the non-gastric H + /K + ATPase ATP12A/ATP1AL1 in unstimulated and butyrate-stimulated (1 and 10 mM) human myelomonocytic HL-60 cells, to unravel its potential role as putative apoptosis-counteracting ion transporter as well as to test for the effect of the H + /K + ATPase inhibitor SCH28080 in apoptosis. (karger.com)
  • Through the regulation of molecular turnover in the differentiating nucleus by external forces, auxeticity could be a key element in mechanotransduction. (exeter.ac.uk)
  • Detailed understanding of the ontogenetic, cellular and molecular characteristics of functional intervertebral disc (IVD) cell populations is pivotal to the successful development of cell replacement therapies and IVD regeneration. (biomedcentral.com)
  • Successful development of cell replacement therapies and IVD regeneration is crucially dependent on an in-depth understanding of cellular and molecular characteristics of the functional IVD. (biomedcentral.com)
  • This project focused on the molecular and functional characterization of the SCFFBXO38 ubiquitin ligase. (nusl.cz)
  • In this manuscript, we review the current literature regarding how HPV-infected cells avoid apoptosis and the molecular mechanisms involved in these events. (mdpi.com)
  • 2001): Thiazolidinediones and glucocorticoids induce differentiation of human adipose tissue stromal cells: Biochemical, cellular, and molecular analysis. (ankara.edu.tr)
  • Most molecular assays, such as conventional real-time RT-PCR (rRT-PCR), detect total RNA in a sample without differentiating vRNA from +RNA. (cdc.gov)
  • These antibodies are added to live, unfixed cells in a single-step assay that takes only 30 minutes and has no adverse effects on stemness or proliferation. (genengnews.com)
  • Despite its broad use as the most common assay for assessing the pluripotency of stem cells, the teratoma assay has limitations. (genengnews.com)
  • The teratoma assay is also inherently variable, depending on the site of cell inoculation and the mouse strain used. (genengnews.com)
  • Differentiation capacity into cells from all three germ layers was confirmed using the teratoma assay. (biomedcentral.com)
  • MPP+ disturbs the developmental differentiation process of these stem cell derived cultures, which can be rescued by GDNF in this stem cell based assay. (neuroproof.com)
  • Development of an RNA strand-specific hybridization assay to differentiate replicating versus non-replicating influenza A virus. (cdc.gov)
  • We, therefore, developed a strand-specific hybridization (SSH) assay that differentiates between vRNA and +RNA and quantifies relative levels of each RNA species. (cdc.gov)
  • Previously, our laboratory reported in several publications on the development of the Endopep-MS method as an assay for BoNT detection and serotype differentiation [ 3 , 4 ]. (cdc.gov)
  • Characterization of embryonic stem cell-differentiated fibroblasts as mesenchymal stem cells with robust expansion capacity and attenuated innate immunity. (usm.edu)
  • Initially Mouse embryonic fibroblasts (MEF) were grown and arrested in their cell cycle with mitomycin, subsequently mouse embryonic SC (mESC) were expanded and subjected in to a pancreatic cell differentiation protocol of 21 days. (intjmorphol.com)
  • 10. Kamata N, Fujimoto R, Tomonari M, Taki M, Nagayama M, Yasumoto S. Immortalization of human dental papilla, dental pulp, periodontal ligament cells and gingival fibroblasts by telomerase reverse transcriptase. (bvsalud.org)
  • In this point of view, they can compare to adult stem cells, but progenitors are said to be in a further stage of cell differentiation. (wikipedia.org)
  • Some of these transit amplifying neural progenitors migrate via rostral migratory stream to the olfactory bulb and differentiate further into specific types of neural cells. (wikipedia.org)
  • 2012). At later EB stage, most CD43+ cells upregulated the manifestation of CD41a and CD235a, and were enriched for megakaryocyte and erythroid progenitors, respectively (Klimchenko et?al. (bioskinrevive.com)
  • Under the same neural differentiation-promoting conditions, the ability of each cell line to differentiate into neural progenitors varied. (biomedcentral.com)
  • DN1 stage) T-cell progenitors. (lu.se)
  • Proliferation-linked shifts in eligibility of T-cell progenitors, population dynamics, DN1 cells to undergo specification thus control kinetics of T-cell generation. (lu.se)
  • In detail, thymus-settling progenitors enter the thymus and generate early T-cell progeni- tors (ETP/DN1 stage), which in turn give rise to DN2, DN3 and DN4 cells. (lu.se)
  • In the last decade, there has been an increased desire for the characterization of this precursor from differentiating hESCs using several methods, generally through three-dimensional embryoid body (EB) differentiation (Ditadi et?al. (bioskinrevive.com)
  • Embryoid bodies (EBs) were obtained from mESC, with different morphological characteristics according to their differentiation, which expressed endodermal germ line genes (sox-17 y pdx-1) at days 0, 11 and 17 of differentiation, an inductor gene of embryonic pancreas development (pdx-1) was detected at day 11 of differentiation. (intjmorphol.com)
  • Many existing tissue-engineering approaches focus on delivery of selected proteins (growth factors, transcription factors etc.) or nucleic acids to host or implanted stem cells to achieve lineage specific differentiation. (frontiersin.org)
  • GS-2 iPSCs showed the capacity to differentiate into cells of the hematopoietic lineage. (biomedcentral.com)
  • 1. Lineage-committed cardiomyocytes differentiated from iPSCs. (creative-bioarray.com)
  • The characterization of naturally occurring mutations and targeted gene deletions has demonstrated that many of these proteins function in terminal differentiation events, including lineage determination, migration, proliferation, survival, and maintenance of mature cell types. (lww.com)
  • Expression of matk mRNA was predominantly found in cells of megakaryocytic lineage. (ox.ac.uk)
  • 2004): Plasticity of human adipose lineage cells toward endothelial perspectives. (ankara.edu.tr)
  • The ability of the host to trigger apoptosis in infected cells is perhaps the most powerful tool by which viruses can be cleared from the host organism. (mdpi.com)
  • To avoid elimination by this mechanism, human papillomaviruses (HPV) have developed several mechanisms that enable the cells they infect to elude both extrinsic and intrinsic apoptosis. (mdpi.com)
  • Many of the current efforts regarding anti-cancer drug development are focused on directing tumor cells to undergo apoptosis. (mdpi.com)
  • In a different study, in which researchers systematically analyzed mutations identical to those found in patients with Darier disease, mutant SERCA2 protein aggregates were found to cause stress to the endoplasmic reticulum, subsequently inducing cell apoptosis. (medscape.com)
  • It helps control the growth and division (proliferation) of cells, the process by which cells mature to carry out specific functions (differentiation), cell movement, and the self-destruction of cells (apoptosis). (medlineplus.gov)
  • The results of these analyses revealed that the differentially expressed exosomal miRNAs participate in multiple biological processes, such as gene expression, synthesis of biomolecules, cell development, differentiation, and signal transduction, among others. (hindawi.com)
  • This identified IOX1, a pan-histone demethylase inhibitor, which selectively downregulates α-globin expression without perturbing erythroid differentiation or general gene expression, more specifically β-like globin expression. (haematologica.org)
  • However, a profound variation in colony morphology, growth rate, BrdU incorporation, and relative abundance of gene expression in undifferentiated and differentiated states of the cell lines was observed. (biomedcentral.com)
  • Analysis of the gene expression patterns in the differentiation process, cells initially showed genetic characteristics of endoderm and thereafter from day 17 of differentiation characteristics of early pancreatic cells which by day 21 of differentiation expressed insulin and glucagon proteins. (intjmorphol.com)
  • The terms "progenitor cell" and "stem cell" are sometimes equated. (wikipedia.org)
  • The kind of potency they have depends on the type of their "parent" stem cell and also on their niche. (wikipedia.org)
  • Advertorial: Pluripotent Stem Cell Verification: Is There an Alternative to Teratoma Assays? (genengnews.com)
  • To address these difficulties, R&D Systems has developed new tools that aid in the verification of stem cell pluripotency during derivation, maintenance, and expansion. (genengnews.com)
  • The GloLIVE™ Human Pluripotent Stem Cell Live Cell Imaging Kit and individual GloLIVE antibodies available from R&D Systems allow you to verify the pluripotency status of your stem cells by monitoring the expression of both positive and negative markers ( Figure 1 ). (genengnews.com)
  • This kit, used in combination with the analysis of colony morphology, promotes the selection of high-quality, undifferentiated stem cell colonies, thereby reducing wasted time. (genengnews.com)
  • Pluripotency of live human stem cell colonies was verified using a NorthernLights™ (NL) 493-conjugated Mouse Anti-Human SSEA-4 Monoclonal Antibody (green) and a NL557-conjugated Mouse Anti-Human TRA-1-60(R) Monoclonal Antibody (red). (genengnews.com)
  • Stem cell therapy in particular is an innovative new field of scientific investigation and clinical application that holds promise for a variety of diseases in veterinary medicine as well as human medicine. (vin.com)
  • A stem cell is a generic term referring to any unspecialized cell that is capable of long-term self-renewal through cell division but that can be induced to differentiate into a specialized, functional cell. (vin.com)
  • For most studies, the adult stem cell in question is actually a mesenchymal stem cell or mesenchymal stromal cell. (vin.com)
  • In addition, it is also important to keep the immune modulatory properties of MSC in mind when interpreting the results of many stem cell therapy studies. (vin.com)
  • To characterize RGCs in human embryonic stem cell (hESC) derived retinal organoids we examined RGC markers and surface antigen expression and made comparisons to human fetal retina. (caltech.edu)
  • Human neuronal stem cell and induced pluripotent stem cell, iPSC, cultures are of high scientific and practical interest in drug discovery, toxicology and for future therapeutic applications. (neuroproof.com)
  • We compare stem cell-derived neuronal network activity patterns with primary cell recordings. (neuroproof.com)
  • GDNF rescues MPP+ effects in human stem cell cultures and primary ventral midbrain cultures from mouse. (neuroproof.com)
  • They are a feasible and physiologically relevant alternative to embryonic stem cell, primary cells, and animal models. (creative-bioarray.com)
  • He has an extensive track-record in quantitative proteomics, introducing metabolic stable isotope labeling in multicellular organisms such as Drosophila and C. elegans, using SILAC for studying stem cell and B cell differentiation, and stable isotope labeling by using chemical approaches, for example to follow differential pTyr phosphorylation in differentiating stem cells. (uu.nl)
  • This study aimed to evaluate the effect of mesenchymal stem cell (MSC)-derived exosomes on an immune-induced liver injury model. (biomedcentral.com)
  • 1999): Autologous Mesenchymal Stem Cell-Mediated Repair of Tendon. (ankara.edu.tr)
  • Stem cell properties of human dental pulp stem cells. (bvsalud.org)
  • Isolation and characterization of a population of immature dental pulp stem cells expressing OCT-4 and other embryonic stem cell markers. (bvsalud.org)
  • Stem Cell Res Ther. (lu.se)
  • My group works with translational stem cell biology. (lu.se)
  • The ease of isolation, growth, and transfection of primary mouse myoblasts under the conditions described here expand the opportunities to study muscle cell growth and differentiation using myoblasts from normal as well as mutant strains of mice. (rupress.org)
  • 27. Melchers, F.: Receptors for the regulation of growth and differentiation of murine B-lymphocytes. (uni-mainz.de)
  • Previously, we have shown that maintaining WJMSCs seeded onto DWJM in osteogenic media induces ectodermal differentiation evident by generating CK 19 positive cells. (ku.edu)
  • To achieve this, we ectodermally differentiate Wharton's jelly mesenchymal stem cells (WJMSCs) by seeding these cells onto a three-dimensional decellularized Wharton's jelly matrix (DWJM) and maintaining them in osteogenic differentiation (OD) media. (ku.edu)
  • In this study, we verified that exosomes derived from osteogenically differentiated ADSCs can promote osteogenic differentiation of ADSCs. (hindawi.com)
  • Moreover, we found that these differentially expressed exosomal miRNAs connect osteogenic differentiation to processes such as axon guidance, MAPK signaling, and Wnt signaling. (hindawi.com)
  • This study confirms that alterations in the expression of exosomal miRNAs can promote osteogenic differentiation of ADSCs, which also provides the foundation for further research on the regulatory functions of exosomal miRNAs in the context of ADSC osteogenesis. (hindawi.com)
  • Human mesenchymal stem cells (HMSCs) are multipotent somatic stem cells that can be isolated from a variety of tissues such as the bone marrow, adipose tissue, and dental pulp. (frontiersin.org)
  • Mesenchymal stem cells are multipotent but not pluripotent, which means they can differentiate into some, or "multiple," but not all tissue types. (vin.com)
  • Adipose tissue (AT) represents a commonly used source of mesenchymal stem/stromal cells (MSCs) whose proregenerative potential has been widely investigated in multiple clinical trials worldwide. (mdpi.com)
  • The extraction, characterization and use of DWJM in skin tissue engineering as a bioactive, biocompatible and biodegradable scaffold were demonstrated. (ku.edu)
  • The fact that these 12 genes had visible effects only once cells had started differentiating and the organoid had started taking on the form of a small brain suggests that these genes have tissue-specific functions, Knoblich says. (the-scientist.com)
  • The IER3IP1 peptide seems to block premature differentiation by promoting secretion of extracellular matrix proteins that support tissue integrity and neural progenitor proliferation. (the-scientist.com)
  • Here we report the generation of clonal NP cell lines from nondegenerate human IVD tissue and present a detailed characterization of NP cellular subpopulations. (biomedcentral.com)
  • The initial stages of DDD correlate with reduced cellularity, aberrant cell function, loss of proteoglycans and concomitant tissue dehydration [ 6 ]. (biomedcentral.com)
  • In total, 1-10% of nucleated cells in adipose tissue are ADSCs, whereas only 0.0001-0.01% of nucleated cells in bone marrow are stem cells ( 5 ). (spandidos-publications.com)
  • Tissue infiltration by activated innate immune cells, including monocytes, macrophages, and neutrophils, if untreated, leads to rapidly progressive organ injury, that can involve many organ systems. (nature.com)
  • 2002): Improvement of postnatal neovascularization by human adipose tissue-derived stem cells. (ankara.edu.tr)
  • Here, we present spatial transcriptomics analysis of fully differentiated grafts in their host tissue. (lu.se)
  • Pulp cells cultures were established by two approaches: enzyme digestion (3 mg/mL type I colagenase and 4 mg/mL dispase), or culture of the tissue explants in cell culture dishes. (bvsalud.org)
  • Dental pulp tissue engineering with stem cells from exfoliated deciduous teeth. (bvsalud.org)
  • The second theory holds that the serosal epithelium of the peritoneum undergoes metaplastic differentiation into endometrium-like tissue. (medscape.com)
  • We identified 24 common genes that were significantly altered in both PMNs and PBMCs, including genes involved in IFN signaling that were downregulated after treatment in both cell populations, and IL1R1 that was upregulated. (frontiersin.org)
  • Their approach combines brain organoids with two other technologies-CRISPR-Cas9 to knock out specific genes and DNA barcoding to track individual cells and their progeny. (the-scientist.com)
  • The team found 13 genes with obvious roles in organ growth-genes that would show up in a 2-D screen of cells as well as the team's 3-D organoid system, Knoblich says-plus an additional 12 that only influenced phenotype later in an organoid's development. (the-scientist.com)
  • In: 'Regulation of the Immune System: Genes and the Cells in which They Function,' Vol. VI, eds. (uni-mainz.de)
  • POU domain factors can exert critical functions in cell types in which they exhibit either transient or sustained expression patterns, and it is likely that their target genes encode receptors, guidance molecules, and neuropeptides that define differentiated phenotypes. (lww.com)
  • Pancreas genes (ins-1 e ins-2) were expressed at day 17 and 21 of differentiation. (intjmorphol.com)
  • The BRAF , MAP2K1 , MAP2K2 , and KRAS genes provide instructions for making proteins that work together to transmit chemical signals from outside the cell to the cell's nucleus. (medlineplus.gov)
  • This finding could also be used to develop improved methods of targeted cellular differentiation of stem cells for therapeutic and regenerative medicine. (umd.edu)
  • With the in-depth researches of MSCs, an increasing number of studies have indicated that the therapeutic effects of MSCs can be attributed not only to their differentiation capacity but also to their paracrine action [ 21 ]. (hindawi.com)
  • The therapeutic effect of umbilical cord-derived mesenchymal stem cells(hUC-MSCs) in combination with pirfenidone(PFD) on pulmonary fibrosis in mice and its possible mechanism were investigated. (researchsquare.com)
  • The comparison of the unique properties and behavior of each individually derived cell line is critical in identifying the safe and efficacious lines for research and therapeutic use [ 3 , 13 ]. (biomedcentral.com)
  • In the present study, the potential of using exosomes isolated from adipose‑derived stem cells (ADSCs) as a therapeutic tool for reducing chronic inflammation and promoting chondrogenesis was investigated using patient‑derived primary cells. (spandidos-publications.com)
  • The above results suggest the need to continue with the study of differentiation protocols to determine the ESC behavior under differentiation and their potential therapeutic use by implementing differentiated cells in animal models. (intjmorphol.com)
  • Embryonic stem cells (ESCs) can grow infinitely and give rise to all types of cells in human body, thus of tremendous therapeutic potentials for a variety of diseases, such as Parkinson's disease, spinal cord injury, and diabetes. (benthamscience.com)
  • Here, we describe a 7-day protocol to differentiate up to 55 million vascular endothelial cells (viECs) from 3.5 million human iPSCs using small molecules to regulate specific transcription factors. (duke.edu)
  • Creative Bioarray provides top quality service for directed-differentiation of iPSCs into cardiomyocytes using our proprietary induction protocol and reagents. (creative-bioarray.com)
  • We developed a novel, serum-free and fully defined differentiation protocol to make BMECs from iPSCs. (vanderbilt.edu)
  • Mesenchymal stem cells (MSCs) are multipotent cells with regenerative and immunomodulatory properties. (frontiersin.org)
  • Overall, the results presented in this study show a common mechanism of endocytosis for MSC EVs across different cell types and the feasibility to generate functionally enhanced EVs by modifications to parental MSCs. (frontiersin.org)
  • Mesenchymal stem cells (MSCs) have immunomodulatory properties. (nature.com)
  • MSCs are multipotent cells characterized by their regenerative capabilities and immunomodulatory properties. (nature.com)
  • 11 The regulatory effect of MSCs on T cells has also been demonstrated in mice models of autoimmune disease such as rheumatoid arthritis and sjogren syndrome (SS). (nature.com)
  • Over the last decades, mesenchymal stem cells (MSCs) have attracted extensive attention in the field of bone regeneration [ 4 - 7 ]. (hindawi.com)
  • The suppressive effects of MSC-derived exosomes and the MSCs in this injury model were evaluated by the level of plasma alanine aminotransferase (ALT), histopathological examinations, the messenger RNA (mRNA) expression of pro- and anti-inflammatory cytokines, and the population alteration of regulatory T cells (Treg) among non-parenchymal liver cells (NPCs). (biomedcentral.com)
  • 2013): Ultrastructural characteristics of sheep and horse mesenchymal stem cells (MSCs). (ankara.edu.tr)
  • 2013): Mesenchymal stem cells (Mscs) in scanning electron microscopy (SEM) world. (ankara.edu.tr)
  • Our data set up that hemogenic endothelium populations endowed with primitive and definitive hematopoietic potential are specified simultaneously from your mesoderm in differentiating hESCs. (bioskinrevive.com)
  • 2014). Using OP9 stromal cells to differentiate hESCs, both Rafii et?al. (bioskinrevive.com)
  • These unique capabilities make hESCs a renewable source of a wide range of cell types for potential use in research and cell-based drug screening and therapies for many diseases. (biomedcentral.com)
  • Unlike earlier transcriptomics analyses using single-cell technologies, we observe that cells derived from human embryonic stem cells (hESCs) in the grafts adopt mature dopaminergic signatures. (lu.se)
  • To date, a great deal of data describing the emergence of blood cells from human being HE have been acquired using stromal co-culture protocols Ezetimibe enzyme inhibitor (Choi et?al. (bioskinrevive.com)
  • 2006). Using EB differentiation protocols, the onset of hematopoietic commitment was also defined from the manifestation of CD43, growing from a CD34+ endothelial precursor human population (Kennedy et?al. (bioskinrevive.com)
  • These monocytes isolation protocols can contribute to the development of reliable methods for the purification of human dendritic cells for research and clinical applications. (jove.com)
  • The main advantage of these protocols is that they facilitate the isolation of human monocytes and their differentiation into viable and functional monocyte derived cells. (jove.com)
  • Having more than five years of experience with hiPSC neuron recordings, we optimize your culture protocols based on functional activity readouts and compare their activity with commercially available reference cell lines. (neuroproof.com)
  • Moreover, the combination of gene modification and directed differentiation of ESCs provides perfect tool for disease modelling and drug discovery. (benthamscience.com)
  • What is more, by deriving NT-ESCs from patient cells, the problem of immune rejection may be avoided. (benthamscience.com)
  • It is also essential to understand how the inherited variation in the sex, stage, quality and genetic background of embryos, as well as environmental influences such as derivation methods and passage procedures can affect the ability of hES cell lines to self-renew and differentiate. (biomedcentral.com)
  • In the present study, a side-by-side comparison of the ability to maintain an undifferentiated state and to self-renew under standard conditions, the ability to spontaneously differentiate into cell types of three germ layers in embryonic bodies, and directed differentiation under neural differentiation-promoting conditions was made between three NIH registered hESC lines I3, I6 and BG01V. (biomedcentral.com)
  • Furthermore, by using this technology we also investigate embryonic stem cells that self-renew in a state of naive pluripotency in which they are competent to generate all somatic cells. (exeter.ac.uk)
  • 2006). Moreover, in both these experimental strategies, the hemogenic potential of endothelium precursor people has been examined at differing times from the differentiation procedure, with or with out a prior purification stage of this people (Ditadi et?al. (bioskinrevive.com)
  • Intracellular markers include transcription factors that control CD4 + T cell differentiation as well as signature cytokines as they traffic through secretory organelles. (rndsystems.com)
  • 22. Melchers, F.: Immunoglobulin synthesis and mitogen reactivity: markers for B lymphocyte differentiation. (uni-mainz.de)
  • 2009): Characterization of mesenchymal stem cells from rat bone marrow: potential and immunophenotypic markers. (ankara.edu.tr)
  • These findings further support the preferred environmental niche of TH-positive cells and confirm their dopaminergic phenotype through the presence of multiple dopaminergic markers. (lu.se)
  • 20. Melchers, F.: Biochemical characterization of immunoglobulin-producing B-lymphocytes at different stages of their differentiation.In: 'Regulation of Growth and Differentiated Function in Eukaryotic Cells,' eds. (uni-mainz.de)
  • To date, cell state and function has primarily been probed by investigating biochemical properties. (exeter.ac.uk)
  • 2007): Chondrogenesis, osteogenesis and adipogenesis of canine mesenchymal stem cells: a biochemical, morphological and ultrastructural study. (ankara.edu.tr)
  • The large number of macrophages in the intestinal tract, play a significant role in maintaining the homeostasis of microorganisms on the surface of the intestinal mucosa and in the continuous renewal of intestinal epithelial cells. (frontiersin.org)
  • 4,6,7 It has been proposed that some of these MSC may actually differentiate into functional renal tubular epithelial cells, though this theory remains controversial. (vin.com)
  • Studies of viral attachment to cultured gastrointestinal epithelial cells (Caco-2) using recombinant virus-like particles or infectious noroviruses indicate that specific histo-blood group antigens play a key role in the attachment of the virus to the host cells ( 13 - 17 ). (cdc.gov)
  • CD4 + T cell subsets exert diverse functions due to their expression of characteristic cytokines. (rndsystems.com)
  • The properties of these cells after transplantation--the stability of resulting hybrid myofibers without immune suppression, the persistence of transgene expression, and the lack of tumorigenicity--suggest that studies of cell-mediated gene therapy using primary myoblasts can now be broadly applied to mouse models of human muscle and non-muscle diseases. (rupress.org)
  • Our data show that selective silencing of α-globin expression in erythroid cells is pharmacologically feasible, and IOX1 is a lead compound to developing new therapy to treat β-thalassemia through the novel pathway of downregulating α-globin expression. (haematologica.org)
  • NP cells were immortalized by simian virus 40 large T antigen (SV40LTag) and human telomerase reverse transcriptase expression. (biomedcentral.com)
  • Differential cell surface marker expression and divergent responses to differentiation conditions suggest that the NP subtypes may correspond to distinct maturation stages and represent distinct NP cell subpopulations. (biomedcentral.com)
  • Functional studies revealed increased expression of ICAM-1 on induced patient-derived endothelial cells (iECs) and of β2-integrins on patient neutrophils that increase neutrophil adhesion and vascular transendothelial migration (TEM). (nature.com)
  • Expression of matk mRNA was up-regulated in megakaryocytic cells induced to differentiate by the phorbol ester. (ox.ac.uk)
  • In this study, we investigated how higher-order chromatin structure modulates differential expression of the human INK4b-ARF-INK4a locus during progenitor cell differentiation, cellular ageing and senescence of cancer cells. (biomedcentral.com)
  • Developmentally regulated EZH2 levels are one of the factors that can determine the higher order chromatin structure and expression pattern of the INK4b-ARF-INK4a locus, coupling human progenitor cell differentiation to proliferation control. (biomedcentral.com)
  • Expression of the INK4b-ARF-INKa locus during cellular ageing and differentiation . (biomedcentral.com)
  • Although the development of cyclosporine A and tacrolimus, which suppress interleukin (IL)-2 expression and T cell-mediated immune responses, was a breakthrough, their adverse effects are still a major problem in the long-term follow-up of transplant recipients. (biomedcentral.com)
  • 2013): S100A1 and S100B Expression Patterns Identify Differentiation Status of Human Articular Chondrocytes. (ankara.edu.tr)
  • The cell population displayed an immunophenotype compatible to the one of stem cells, with remarkable positive expression of CD117. (bvsalud.org)
  • The investigators found that the resultant transfected cells showed defects in ATP2A2 protein expression (15 mutants), ATP hydrolysis (29 mutants), calcium transport (4 mutants), and calcium binding and kinetics (3 mutants). (medscape.com)
  • By transporting "cargos" such as proteins, RNAs, DNAs, and lipids [ 26 ], exosomes regulate the eventual fate of recipient cells. (hindawi.com)
  • Therefore, we aimed to characterize proteomes of the acrosome and acrosome- reacted sperm cells, identify important proteins using advanced bioinformatics approaches and discuss the function of the acrosome as a vesicle that serves to store and process metabolites after the previous maturation process. (nusl.cz)
  • Finally the production of insulin and glucagon proteins was detected on the EBS at day 21 of differentiation. (intjmorphol.com)
  • focuses on the development and implementation of innovative mass spectrometric methods for the more efficient and detailed characterization of proteins in relation to their biological function. (uu.nl)
  • The emphasis is on the structural characterization of proteins and post-translational modifications as well as the investigation of protein complexes and protein interactions important in e.g. protein folding, protein ligand binding, and the formation of tertiary and quaternary structures. (uu.nl)
  • Time and resources spent expanding stem cells are wasted if the resulting population is not functionally pluripotent. (genengnews.com)
  • In this review, I overview our experience with functionally differentiated cultured plant cells and their characteristics, especially with regard to photoautotrophism and secondary metabolism. (go.jp)
  • I emphasize the high potential of functionally differentiated cell cultures, as well as some of the pitfalls, in the characterization of plant functions and biotechnological applications. (go.jp)
  • and keywords Abstract and keywords The ubiquitin-proteasome system (UPS) is a tightly and specifically regulated system of protein degradation in eukaryotic cells. (nusl.cz)
  • They are valuable cells because they directly precede endothelial cells, but have characteristics of stem cells. (wikipedia.org)
  • Using this information, we performed experiments on mESC using the AFM to understand their characteristics in the undifferentiated pluripotent state as well as early differentiating state. (umd.edu)
  • We were able to identify two predominant clonal NP subtypes based on their morphological characteristics and their ability to induce SOX9 and COL2A1 under conventional differentiation conditions. (biomedcentral.com)
  • 11. Kramer PR, Nares S, Kramer SF, Grogan D, Kaiser M. Mesenchymal stem cells acquire characteristics of cells in the periodontal ligament in vitro. (bvsalud.org)
  • The morphological analysis evidenced three-dimensional cell clusters corresponding to EBs. (intjmorphol.com)
  • This study was developed to compare the morphological, proliferative and immunophenotypic profiles of pulp cells from permanent and primary teeth, obtained by two isolation methods. (bvsalud.org)
  • Morphological and proliferative analyses, as well as immunophenotype characterization with monoclonal antibodies against CD117, CD34 and CD45 surface receptors were performed. (bvsalud.org)
  • Kim and Park, 2017 ) and make HMSCs attractive cells for regenerative therapies. (frontiersin.org)
  • Recent years have brought increased interest in the potential for adult stem cells to help in the treatment of many diseases through both their regenerative properties as well as their apparent ability to alter the environment in injured and diseased tissues. (vin.com)
  • Stem cells are emerging as an important source of material for diseases in regenerative medicine. (benthamscience.com)
  • The study of biology of stem cells is the hallmark of the recent emerging field of regenerative medicine and medical biotechnology. (benthamscience.com)
  • Th9 polarized cells are present in low abundance in normal human peripheral blood. (rndsystems.com)
  • Human peripheral blood mononuclear cells (PBMCs) were stained with APC-conjugated Mouse Anti-Human CD19 Monoclonal Antibody (Catalog # FAB4867A ) and either (A) PE-conjugated Mouse Anti-Human CCR6 Monoclonal Antibody (Catalog # FAB195P ) or (B) PE-conjugated Mouse IgG2B Isotype Control (Catalog # IC0041P ). (rndsystems.com)
  • Our results demonstrate that the highly differentiated 3-D cell culture model can support the natural growth of human noroviruses, whereas previous attempts that used differentiated monolayer cultures failed. (cdc.gov)
  • However, these models do not answer the fundamental questions of human norovirus attachment and entry into cells of the human gastrointestinal tract. (cdc.gov)
  • Representative models of differentiated human intestinal epithelium can be established by growing cells in 3 dimensions (3-D) on collagen-I-coated porous microcarrier beads in rotating-wall vessel (RWV) bioreactors that model the physiologic fluid-shear environment in their respective organs ( 21 - 24 ). (cdc.gov)
  • This study compares two different methods of human monocyte isolation for obtaining in vitro dendritic cells (DCs). (jove.com)
  • In this study, we aimed to investigate whether phenotypically distinct clonal cell lines representing different subpopulations in the human NP could be generated using immortalization strategies. (biomedcentral.com)
  • This led to many studies that significantly enhanced our knowledge of the human BBB, but most differentiation schematics use serum in the process. (vanderbilt.edu)
  • Antibodies raised against the NH2 terminus of matk immunoprecipitated a 60-kDa protein from the CMK human megakaryocyte cell line. (ox.ac.uk)
  • The human body is made up of about 220 different kinds of specialized cells such as nerve cells, muscle cells, fat cells and skin cells. (benthamscience.com)
  • 2002): Myogenic differentiation by human processed lipoaspirate cells. (ankara.edu.tr)
  • 2004): Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells. (ankara.edu.tr)
  • 2002): Neurogenic differentiation of murine and human adipose- derived stromal cells. (ankara.edu.tr)
  • SHED: stem cells from human exfoliated deciduous teeth. (bvsalud.org)
  • 9. Chen SC, Marino V, Gronthos S, Bartold PM. Location of putative stem cells in human periodontal ligament. (bvsalud.org)
  • Mesenchymal stem cells or mesenchymal stromal cells (MSC) are plastic adherent and assume a fibroblast-like morphology during culture. (vin.com)
  • 2014), or co-culture on stromal cell lines (Choi et?al. (bioskinrevive.com)
  • 2013). The performance of hematopoietic differentiation differs between your two methodologies because of parameters such as for example serum, stromal maintenance, or EB size, amongst others elements (Kardel and Eaves, 2012, Vodyanik et?al. (bioskinrevive.com)
  • 2 Psoriasis is a disease with innate and adaptive immune system disorder and dendritic cells, neutrophils, keratinocytes and T cells play major roles in the pathogenesis. (nature.com)
  • The lamina propria of the small intestine is the main site of the intestinal immune system, which contains a large number of macrophages, CD4 T cells, and dendritic cells. (frontiersin.org)
  • The chemokine CCL2, which pared to GPT, the LLNA reduces animal numbers needed, can recruit dendritic cells into the site of inflammation, is improves animal welfare, and decreases experimental time also upregulated in keratinocytes following hapten exposure. (cdc.gov)
  • To achieve a proper separation of the peripheral blood mononuclear cells, load the blood onto the density gradient solution quickly, but carefully and without mixing the layers. (jove.com)
  • Moreover, colony morphology is not truly indicative of pluripotency, since cells that are not fully reprogrammed can have similar morphology. (genengnews.com)
  • CT is the primary diagnostic tool for the characterization of an indeterminate renal mass, including both cystic and solid tumors. (bvsalud.org)
  • Magnetic resonance imaging (MRI) is a useful complementary imaging tool for the diagnosis and characterization of renal masses, as it provides both morphologic and functional information. (bvsalud.org)
  • MRI may be applied for renal cystic lesion characterization, differentiation of renal cell carcinoma (RCC) from benign solid renal tumors, RCC histologic grading, staging, post-treatment follow-up, and active surveillance of patients with treated or untreated RCC. (bvsalud.org)
  • renal cell carcinoma has the same appearance. (medscape.com)
  • In a case series with pathologically proven renal cell carcinomas, 32% of tumors with a diameter ≤3 cm were echogenic. (medscape.com)
  • Our results show that the mechanical property of undifferentiated mESC differs from early differentiating (6th day) mESC in both live and fixed cells. (umd.edu)
  • In conclusion, the mESC differentiation was achieved. (intjmorphol.com)
  • Our cell lines yield novel insights into NP biology and provide promising new tools for studies of IVD development, cell function and disease. (biomedcentral.com)
  • To time, it really is still as yet not known whether HE subsets with different hematopoietic potentials emerge in successive waves during hESC differentiation, whether HE populations are preserved inside the differentiating lifestyle as time passes, or whether one exclusive population of He's produced early from mesoderm and steadily differentiates inside the lifestyle. (bioskinrevive.com)
  • The natural aging of cells, called their cellular senescence, is one of the main contributors to aging on an organismal level. (wikipedia.org)
  • Brain microvascular endothelial cells (BMECs) line the capillaries, forming the BBB. (vanderbilt.edu)
  • The fate of the pure myoblast populations after transplantation was monitored by labeling the cells with the marker enzyme beta-galactosidase (beta-gal) using retroviral mediated gene transfer. (rupress.org)
  • Early passage NP monolayer cell cultures were initially characterized using a recently established NP marker set. (biomedcentral.com)
  • Both of these cell populations have disadvantages: myogenic cell lines are tumorigenic, and primary cultures contain a substantial percentage of non-myogenic cells which will not fuse to host fibers. (rupress.org)
  • Furthermore, for both cell populations, immune suppression of the host has been necessary for long-term retention of transplanted cells. (rupress.org)
  • Following hemogenic potential of endothelium cell populations frequently during the period of hESC differentiation would address a few of these problems but to time this has hardly ever been reported. (bioskinrevive.com)
  • They proliferate easily in culture and can be cryopreserved without loss of phenotype or differentiation potential. (vin.com)
  • In addition, cluster of differentiation 24 (CD24)-negative NP responder clones formed spheroid structures in various culture systems, suggesting the preservation of a more immature phenotype compared to CD24-positive nonresponder clones. (biomedcentral.com)