• G-actin regulated genes were induced by treatment with cytochalasin D ( CD , 2 μM, 90 min) and repressed by latrunculin B ( LB , 5 μM). (biomedcentral.com)
  • 0.1 μg/ml) in the presence or absence of cytochalasin D (2 μM), as an actin disrupter. (biomedcentral.com)
  • In the presence of cytochalasin D, cortactin immunostaining was punctate and distributed in a pattern similar to that reported for actin in cells exposed to cytochalasin D. Sertoli cells not exposed to FSH, but activated with beads, did not show cortactin immunostaining around the phagocytized beads at any of the time periods. (biomedcentral.com)
  • The transient increase of Orai1 protein abundance was abrogated by Rac1 inhibitor NSC23766 (50 μM) and by prevention of actin reorganization with cytochalasin B (1 μM). (biomedcentral.com)
  • It was rapidly demonstrated that the toxins target the cytoskeleton and inhibit the actin polymerization by specifically sequestering the G-actin monomers with a high affinity [4] , unlike cytochalasin D that targets the actin filament [5] . (beilstein-journals.org)
  • Differentiation of erythrocyte-(GLUT1), liver-(GLUT2), and adipocyte-type (GLUT4) glucose transporters by binding of the inhibitory ligands cytochalasin B, forskolin, dipyridamole, and isobutylmethylxanthine. (aspetjournals.org)
  • Further molecular characterization of active factors and their cellular targets is warranted on account of the magnitude of the effects reported here and their potential relevance for nervous system repair. (nature.com)
  • This is combined with biochemical characterization of the molecular mechanism in vitro and in vivo. (au.dk)
  • NIH 3T3 cells were treated with cytochalasin D (2 μM) for 120 min, or with cytochalasin following 30 min pretreatment with latrunculin B (5 μM). (biomedcentral.com)
  • Characterization of angiotensin II (AT2) binding sites in R3T3 cells. (aspetjournals.org)
  • Kelly, F. , Sambrook, J. (1974) Variants of Simian Virus 40-Transformed Mouse Cells Resistant to Cytochalasin-B. Cold Spring Harbor Symposia on Quantitative Biology, 39. (cshl.edu)
  • Characterization of the comparison between each group with the photoconvertible fluorescent protein to allow merozoite egress voltaren online purchase. (itwontfailbecauseofme.com)
  • The extent of MDI intracellular protein binding was not affected by cytochalasin D, a chemical that binds actin filaments and inhibits active uptake into cells. (pdx.edu)
  • and 3) binds the chemical cytochalasin B. Aim 2: Determine if glucose transporter 1 (GLUT1) is necessary for overload-stimulated skeletal muscle glucose uptake. (ecu.edu)
  • Characterization of CAR-M activity showed that CAR-Ms expressed pro-inflammatory cytokines and chemokines, converted bystander M2 macrophages to M1, upregulated antigen presentation machinery, recruited and presented antigen to T cells and resisted the effects of immunosuppressive cytokines. (nature.com)
  • Download Handbook of Flavor Characterization: contractile, Chemical and by Kathryn D. This public compliance viewpoint the scaffolding incentives and various pages considered to create out the reference of thinking punishment, structuralists and proper design field on Page and histamine registration. (flyscreenteam.com)
  • Characterization of the glucose transporters responsible for mechanical overloadstimulated glucose uptake in mouse skeletal muscle (Doctoral Dissertation, East Carolina University). (ecu.edu)
  • However, single-modal mechanical or electrical properties alone are not specific enough to characterize single cells accurately, and it has been long and challenging to couple the conventionally image-based mechanical characterization and impedance-based electrical characterization. (bvsalud.org)
  • With multimodal high-dimensional characterization, the electrical-mechanical flow cytometry can better reveal the difference in cell types, demonstrated by the experimental results with three types of cancer cells (HepG2, MCF-7, and MDA-MB-468) with 93.4% classification accuracy and pharmacological perturbations of the cytoskeleton (fixed and Cytochalasin B treated cells) with 95.1% classification accuracy. (bvsalud.org)
  • It is envisioned that multimodal electrical-mechanical flow cytometry provides a new perspective for accurate label-free single-cell intrinsic characterization. (bvsalud.org)
  • The best characterization of dNCO-protein conjugates is achieved by the quantitative determination of conjugated dNCO per mole of protein as well as determining the extent of dNCO cross-linking. (pdx.edu)
  • Vinblastine also inhibits p l a s m a protein secretion but lumicolchicine, griseofulvin, and cytochalasin B do not. (docksci.com)
  • Here we report the cloning and characterization of a novel GAP-binding protein, mTid-1, a DnaJ chaperone protein that represents the murine homolog of the Drosophila tumor suppressor l(2)tid gene. (embl.de)
  • motivating doing 0%)0%1 Prices and vascular document years, the tech of Flavor Characterization opens the information and size of discourse and bank with smooth server from high sale vocabularies emerging following members of request merging readers and in human itself--like page ingredients. (flyscreenteam.com)
  • Finally, an experiment is performed using the laser range finder to test whether the information gained from the characterization of the sensors can produce greater accuracy when exploring a new area with the quadrotor. (upenn.edu)