• The host cells contain macromolecules such as laminin, thrombospondin, heparin sulphate, and fibronectin that cover their surface. (wikipedia.org)
  • Using the human corneal epithelial cell line HCE-T, adhesion to laminin isoforms and fibronectin was determined using a colorimetric adhesion assay. (nih.gov)
  • During liver fibrosis, the composition of the ECM changes from one dominated by collagen IV and laminin [ 4 ], to one of fibrillar collagen, predominantly collagen I and III, as well as fibronectin [ 5 ]. (biomedcentral.com)
  • The molecules and proteins on the cytoskeleton of the cell also bind to the surface of the parasite and initiate host invasion. (wikipedia.org)
  • Following the implantation of a biomaterial into a host tissue, the first event to occur at the tissue-material interface (which dictates biocompatibility) is the noncovalent adsorption of plasma proteins from blood onto the surface [ 517 - 520 , 936 , 954 ] if the material comes into direct contact with blood. (nanomedicine.com)
  • Once proteins have adsorbed to the surface of the foreign material, host cells no longer see this underlying material, but only the protein-coated surface overlayer. (nanomedicine.com)
  • Thus the type and state of adsorbed proteins, including their conformational changes, are critical determinants of biocompatibility [ 518 - 523 ], so pretreatment of surfaces can be a control mechanism ( Section 15.2.2.1 ). (nanomedicine.com)
  • Due to their large surface area and ability to interact with proteins and peptides, graphene oxides offer valuable physiochemical and biological features for biomedical applications and have been successfully employed for optimizing scaffold architectures for a wide range of organs, from the skin to cardiac tissue. (mdpi.com)
  • During the initial infection, alpha-helical M proteins (M8 and M13) on the surface of the streptococcus bind type IV collagen in the host, and this interaction can trigger auto-antibody formation. (medscape.com)
  • A and rot as well as for the surface proteins UafA and Aas. (biomedcentral.com)
  • There he worked with Dr. Max D. Cooper (Howard Hughes Medical Institute, National Academy of Sciences) and his research focused on cell surface proteins expressed by preB cells that regulate B cell maturation and homing. (ubc.ca)
  • In addition, he identified a number of novel hematopoietic stem cell surface proteins and began analyzing their function. (ubc.ca)
  • His laboratory has followed two primary interests: 1) the transcription factor networks that regulate fate determination in various cells that make blood, and 2) the cell surface proteins expressed by hematopoietic stem cells that and allow them to communicate with their microenvironment. (ubc.ca)
  • In this regard, his lab has identified a novel family of hematopoietic cell surface proteins, called the CD34 family, and shown that these are essential for a number of developmentally important processes. (ubc.ca)
  • Immunofluorescence microscopy reveals that alpha-integrin protein localization patterns are not significantly different in HCE-T interacting with EHS or placental laminin. (nih.gov)
  • Protein adsorption is much more rapid than the transport of host cells to foreign surfaces. (nanomedicine.com)
  • This adsorbed protein overlayer - rather than the foreign material itself - then mediates the types of cells that may adhere to the surface, which ultimately can determine the type of tissue that forms in the vicinity [ 517 ]. (nanomedicine.com)
  • Protein adsorption on a range of hydrophobic and hydrophilic polymer surfaces is typically 0.3-12 mg/m 2 (~500-20,000 molecules/micron 2 ) [ 1322 ]. (nanomedicine.com)
  • One common example is fibrinogen, a molecule that is reduced slightly in molecular weight and ultimately converted to the active protein, fibrin, after foreign surface contact during blood clotting ( Section 15.2.5 ). (nanomedicine.com)
  • Further, biophysical experiments suggested that HomA and HomB are dimeric and most likely the cysteine residues present on surface-exposed loops participate in protein-protein interactions. (nature.com)
  • In addition, Sive lab researchers demonstrated that this process requires the protein laminin, which is a crucial part of the basement membrane that lines the outside of the neural tube. (news-medical.net)
  • Hemidesmosome formation and positive labeling for integrins alpha6 and beta4 was observed after 14-15 days, whereas deposition of basement membrane material and positive labeling for type IV collagen and laminin-1 occurred after 17-20 days. (arvojournals.org)
  • This could be considered to be part of a feedback loop as changes in ECM composition results in changes in HSC cell surface receptors, particularly integrins indicating that the ECM should not be regarded simply as an inert physical scaffold but a dynamic structure that is able to influence the phenotype of HSCs [ 2 , 8 , 9 ]. (biomedcentral.com)
  • TBD cells embedded in collagen gel formed three-dimensional clusters, which had an internal cavity equipped with a tight junction-like structure, a microvilluslike structure, and a laminin-positive layer surrounding the cluster. (bioone.org)
  • Nearly 90% of the clinical isolates were found to possess the gene for the surface-associated lipase Ssp and 10% for the collagen binding MSCRAMM SdrI. (biomedcentral.com)
  • HSCs express numerous cell surface receptors including both discoidin domain receptors (DDRs)-which are receptors for collagen I and many of the integrin subunits, which are a major family of cell surface receptors for extracellular molecules and play a critical role in many biological functions [ 7 , 10 ]. (biomedcentral.com)
  • These studies demonstrate that human corneal epithelial cells are capable of rapid adhesion to, and enhanced spreading on, laminin isoforms not characteristically resident in the adult corneal basement membrane. (nih.gov)
  • MCF-10CA1a cells grown in three-dimensional basement membrane culture form large, hyper-proliferative masses, that retain few characteristics of MCF10A acini and more closely resemble tumors. (biomedcentral.com)
  • The mechanical kinetics of adsorption have also been examined by testing the adhesion strength of an individual fibrinogen molecule that is affixed to an AFM tip and is briefly touched to a silica surface [ 566 ]. (nanomedicine.com)
  • At high solution concentrations, the molecule forms end-to-end polymers on hydrophilic surfaces and spherical 18-24 nm diameter structures on hydrophobic surfaces [ 565 ]. (nanomedicine.com)
  • This is comparable to the characteristic distance between D and E globules of a single fibrinogen molecule, suggesting that fibrinogen adsorbs mainly through its D and E globules [ 566 ]. (nanomedicine.com)
  • A highly pure preparation of mouse laminin that is devoid of the bridging entactin molecule. (corning.com)
  • In short-term adhesion assays, the preferred substrate for HCE-T attachment is placental laminin. (nih.gov)
  • Corning BioCoat™ PDL/Laminin enhances the attachment, propagation and differentiation of neuronal cell on plastic and glass surfaces. (corning.com)
  • The trypomastigotes are in the feces and are capable of swimming into the host's cells using flagella, a characteristic swimming tail dominant in the Euglenoid class of protists. (wikipedia.org)
  • To analyze alpha-integrin mediated adhesion of human corneal epithelial cells to placental and EHS laminin isoforms. (nih.gov)
  • This result suggests that co-cultured TBD cells exhibited a characteristic of Type III taste cells. (bioone.org)
  • Culturing cells in or on gels such as Laminin/Entactin Complex enable the study of specific mechanisms that dictate cell differentiation and functionality. (corning.com)
  • Cells cultured on a Laminin surface exhibit a spindle-shaped morphology and dendritic processes. (corning.com)
  • Rat PDL DPCs also exhibited differentiative potential characteristic of stem cells. (nih.gov)
  • To characterize the phenotype of human limbal epithelial cells expanded on a standardized biological substrate with regard to morphology, growth potential, and antigen expression patterns, and to evaluate their clinical application in corneal surface reconstruction. (arvojournals.org)
  • Fibrin-cultured cells retained characteristics similar to those of the limbal stem cell compartment in situ. (arvojournals.org)
  • Some of these molecules are glycoproteins expressed on the cell surface, socalled cell adhesion molecules (CAM), which mediate either the contact between two cells or the contact among cells and the extracellular matrix, being therefore of fundamental importance for adhesion 3 . (bvsalud.org)
  • alpha-integrin sub-unit modulation of corneal epithelial cell interaction with laminin isoforms was analyzed using immunofluorescence microscopy and adhesion assays incorporating functional blocking antibodies. (nih.gov)
  • This characteristic of human corneal epithelium may explain, at least in part, why amniotic membrane transplantation is proving to be clinically useful for human ocular surface reconstruction. (nih.gov)
  • After standardization of the technique, fibrin-cultured autologous epithelial sheets were transplanted onto the corneal surface of 5 patients with limbal stem cell deficiency due to chemical burns. (arvojournals.org)
  • Western blot analysis was used to partially characterize commercially available preparations of laminin isolated from the mouse EHS sarcoma and from human placenta. (nih.gov)
  • A clearer molecular picture of the fibrinogen adsorption event on implant surfaces (and subsequent inflammatory response) is slowly emerging [ 567 , 568 ]. (nanomedicine.com)
  • Analyses of recovered implants revealed organized PDL tissues surrounding titanium implant surfaces in PDL-cell-seeded, and not in unseeded control, implants. (nih.gov)
  • As a result of this coating process, however, the material increased in surface roughness, which may have an adverse effect on the prevention of bacterial infection. (elsevierpure.com)
  • In the present study, a reduction of the surface roughness of the laminin-apatite composite layer was attempted by adding MgSO 4 to the LCP solution as an inhibitor of apatite crystal growth. (elsevierpure.com)
  • The surface roughness, Ra, of the resulting laminin-apatite composite layer decreased from approximately 0.43 to 0.31 μm upon increasing the MgSO 4 concentration in the LCP solution from 0 to 1.5 mM, while retaining its cell adhesion characteristics. (elsevierpure.com)
  • The thus-obtained material, having a laminin-apatite composite coating with reduced surface roughness, has potential as a material for percutaneous devices with improved resistance to bacterial infection through the interface between the device and the skin. (elsevierpure.com)
  • The use of nanotechnology plays an important role in tissue engineering because the properties that this technology adds to the material, such as the greater surface area and greater roughness, improve the physical-chemical properties which mimic those of the natural tissues and organs 31 . (bvsalud.org)
  • Individual triatomines infected with protozoa from other contact with animals transmit trypanosomes when the triatomine deposits its faeces on the host's skin surface and then bites. (wikipedia.org)
  • The limitations of interfacing electrodes including low charge injection limits, mechanical mismatch and foreign body response can be addressed through the use of organic electrode coatings which typically provide a softer, more roughened surface to enable both improved charge transfer and lower mechanical mismatch with neural tissue. (frontiersin.org)
  • Taste buds are the end organs of taste located in the gustatory papillae, which occur on the surface of the oral cavity. (bioone.org)
  • This is very important in forming tubes, which are found in essentially all organs, and constrictions, which bend many organs in characteristic ways. (news-medical.net)
  • The remainder of this article addresses the key clinical characteristics and pathologic findings on muscle biopsy of selected examples of disorders from 4 different categories of muscle disease: immune-mediated (inflammatory) myopathies, muscular dystrophies, metabolic myopathies, and congenital myopathies. (medscape.com)
  • Human bmMSCs were described in the late nineties as well [ 6 ] and at the same time a breakthrough study investigated the expression of typical cell surface markers and the proliferation and differentiation properties of human MSCs in more detail [ 7 ]. (hindawi.com)
  • Use Corning Laminin surfaces as a thin coating on tissue culture surfaces or as a soluble additive to culture medium to promote cell adhesion, migration, chemotaxis, growth, and differentiation. (corning.com)
  • Most metallic electrodes inject charge through the generation of electrons at the electrode surface, however in physiological systems charge is carried by electrolytes (ions). (frontiersin.org)
  • Suitable as a thin coating on tissue culture surfaces or as a soluble additive to culture medium. (corning.com)
  • Variants of leukocyte adhesion deficiency have also been reported, including fully expressed but nonfunctional CD18 and an E selectin that is expressed but rapidly cleaved from the cell surface (only present in soluble form). (medscape.com)
  • In this study, we aimed to demonstrate the glycohistochemical characteristics of the LTM in the syrinx of the Denizli cock. (9lib.net)
  • In total, 236 clinical isolates and 15 animal isolates of S. saprophyticus were characterized in respect of the occurrence of 9 potential virulence genes and four surface properties. (biomedcentral.com)
  • Here 236 clinical isolates of S. saprophyticus and 15 isolates of animal origin were characterized regarding the existence of these genes and surface properties. (biomedcentral.com)
  • But, the researchers found that basal constriction takes place even in zebrafish mutants that fial to inflate their cavities, so that folding of the tissue is actively controlled, at least in part, from the basal surface. (news-medical.net)
  • PDGFRb is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. (thermofisher.com)
  • On quartz at low solution concentrations, fibrinogen molecules appear to form a 46-nm long multinodular rod with 6-7 nodes each 4 nm in diameter on hydrophilic surfaces, and a 40-nm long binodular or trinodular rod with node diameter 5-9 nm on hydrophobic surfaces. (nanomedicine.com)
  • Other unusual features of the receptor include its ability to engage classes of molecules, rather than individual ligands, and its enhanced surface expression in environments rich in RAGE ligands. (jci.org)
  • An artificial material coated with a laminin-apatite composite layer on its surface would be useful as a percutaneous device with cell-adhesion properties and good biocompatibility. (elsevierpure.com)
  • In the severe PE group, complementary measurements of platelet Annexin V- and CD62 (P-Selectin) surface expression showed apoptosis of platelet populations in the majority of patients. (bvsalud.org)
  • This is likely related to circulating subtypes of streptococci and genetic characteristics of the host populations. (medscape.com)
  • individuals with this condition typically have a brain abnormality called cobblestone lissencephaly, in which the surface of the brain lacks the normal folds and grooves and instead develops a bumpy, irregular appearance (like that of cobblestones). (medlineplus.gov)
  • Many genes and characteristics were investigated for the staphylococcal species S. aureus and S. epidermidis , and the distribution of potential virulence factors among infectious isolates have been studied extensively. (biomedcentral.com)
  • Of the theoretical training needs, topics related to pharmacokinetics and special characteristics of using medicines in the elderly, effects, adverse effects and interactions of medicines, were most important. (helsinki.fi)
  • In its presence, growth factors either adsorb or moisten the surface of the bone substitutes, promoting an adequate integration with the host bone 13 . (bvsalud.org)
  • The present authors recently prepared such a composite by a liquid-phase coating process in which a surface-modified material was immersed in a laminin-containing calcium phosphate solution (LCP solution) supersaturated with respect to apatite. (elsevierpure.com)
  • NG-108 rat glioma/mouse neuroblastoma cell morphology is surface dependent. (corning.com)
  • 1987 ). This superfamily of proteases is defined by the presence of a Zn 2+ ion at the catalytic center, which is coordinated by three histidine residues in the zinc-binding consensus sequence HExxHxxGxxH that is present in all proteolytically active metzincins, and a characteristic, strictly conserved methionine-containing tight 1,4 beta turn forming a hydrophobic cleft for the catalytic zinc ion (Bode et al. (evelinvahter.com)
  • Laminin/Entactin Complex High Concentration (HC) is a special formulation that Corning has developed to enhance three-dimensional (3D) cell culture applications. (corning.com)
  • Training needs also covered special characteristics of pharmacotherapy for the elderly, and formulations and administration routes of medicines. (helsinki.fi)
  • Interestingly, the in-silico analysis revealed that HomA/B consists of 8 discontinuous N and C terminal β-strands forming a small β-barrel, along with a large surface-exposed globular domain. (nature.com)
  • Contact with foreign surfaces, along with adhesive forces, may cause premature activation. (nanomedicine.com)
  • PDGFRb is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. (thermofisher.com)