• Using single-cell elemental analytical methods at the Argonne National Laboratory in collaboration with inorganic chemist Tom O'Halloran, Woodruff discovered a role of zinc in the regulation of oocyte maturation and at the moment of fertilization. (wikipedia.org)
  • The present review discusses methods to improve oocyte quality in older women and new stimulation protocols that may improve the number of mature oocytes retrieved during an in vitro fertilization cycle. (arvigen.com)
  • Usage of melatonin during in vitro fertilization (IVF) procedures has been found to improve oocyte quality, survival, and fecundity. (jrhm.org)
  • On the one hand, reactive oxygen species (ROS) are mandatory mediators for essential cellular functions including the function of germ cells (oocytes and spermatozoa) and thereby the fertilization process. (hindawi.com)
  • However, these techniques have to be carried out very carefully as they, if applied wrongly, bear risks of generating ROS damaging the germ cells and preventing fertilization. (hindawi.com)
  • This has been shown for intrauterine insemination (IUI), in vitro fertilization (IVF), and ICSI, and increased incidences of chromosomal abnormalities, minor and major birth defects, or early childhood cancers, particularly in the male offspring, have been linked to these procedures [ 8 - 16 ]. (hindawi.com)
  • SECM technique may be a valuable tool for accurately assessing the quality of embryos and thereby contribute to improving outcomes associated with assisted reproduction, including human in vitro fertilization. (bioone.org)
  • A retrospective analysis was conducted on 1630 individuals with endometriosis who underwent in vitro fertilization (IVF) with fresh embryo transfer at the Reproductive Medicine Center of Fujian Maternity and Child Health Hospital from January 2018 to January 2022. (biomedcentral.com)
  • Assisted reproductive techniques for canines: preservation of genetic material in domestic dogs [3] "Assisted reproductive techniques (ARTs), such as artificial insemination, in vitro fertilization, and cryopreservation of gametes/zygotes, have been developed to improve breeding and reproduction of livestock and for the treatment of human infertility. (edu.au)
  • In vitro Fertilization of retrieved oocytes with recipient's husband's sperm /Donor sperm obtained from Sperm bank. (lushfertility.in)
  • Despite considerable technological advancements, polyspermy remains a significant challenge in in-vitro fertilization (IVF) procedures in pigs, disrupting normal embryonic development. (e-jarb.org)
  • In total, 789 patients who underwent OPU procedures for in vitro fertilization (IVF) were included in the study. (ecerm.org)
  • In vitro fertilization (IVF), in which fertilization takes place after mature eggs are collected from the ovaries, is a widely used method in the treatment of infertility [ 3 ]. (ecerm.org)
  • Dr. Akerman specializes in the hallmark of Assisted Conception: In Vitro Fertilization (IVF). (drfernandoakerman.com)
  • This study was carried out in a private IVF centre in conjunction with Assisted Reproductive Techniques Center of Ain Shams University Maternity Hospital after the approval of the Research Ethics Committee, during the period between Jan 2014 to Jan 2016 and included 60 women, with unexplained infertility undergoing in vitro fertilization (IVF), were randomised into two groups. (peertechzpublications.com)
  • The ampulla serves as the physiologic site for final gamete maturation, fertilization, and early embryonic development. (medscape.com)
  • The aim of this study is to investigate the role of activated ovarian endothelial cells in early in-vitro follicular development. (biomedcentral.com)
  • Beginning on day 4 of culture, we observed significantly higher follicular and oocyte growth rates in follicles co-cultured with AOECs compared with follicles on gel or matrigel. (biomedcentral.com)
  • This model is a novel platform for the in vitro maturation of early follicles and for the future exploration of endothelial-follicular communication. (biomedcentral.com)
  • Duplicating the complex interaction of growth factors, cellular and hormonal signals required for follicular development and oocyte maturation in vitro is challenging. (biomedcentral.com)
  • The mammalian ovary has all the molecular machinery required for the biosynthesis of melatonin and interestingly concentration of melatonin in the follicular fluid of pre-ovulatory follicles is much higher than circulatory melatonin even in humans. (jrhm.org)
  • Further, melatonin and its receptors play a significant role in antioxidant defense mechanism of ovary for follicular growth and maturation. (jrhm.org)
  • At the end of 15 days, five mice in each group were euthanized and the ovaries were analyzed for follicular morphometry and granulosa cell count. (biomedcentral.com)
  • Prior to the onset of menopause (10-15 y), menstrual cycles shorten and rapid follicular loss occurs because more oocytes are being recruited. (medscape.com)
  • Most IVF centers will therefore, monitor follicular size and administer the trigger of oocyte maturation once follicles are deemed to have grown to an appropriate size. (plandl.best)
  • On March 28, 2017, her team announced the creation of Evatar, a miniaturized female reproductive tract composed of ovarian follicles or intact ovaries (mouse) interconnected with human explants from fallopian tubes, uterus, and cervix with liver organoids to provide a metabolic management tissue system. (wikipedia.org)
  • In vitro development of early follicles necessitates a complex interplay of growth factors and signals required for development. (biomedcentral.com)
  • These drawbacks could be overcome by the ability to mature early follicles in vitro. (biomedcentral.com)
  • In mammals , the first part of oogenesis starts in the germinal epithelium , which gives rise to the development of ovarian follicles , the functional unit of the ovary . (wikipedia.org)
  • As follicles grow, they acquire receptors for luteinizing hormone, a pituitary hormone that reinitiates meiosis in the oocyte and causes ovulation of a fertilizable egg. (wikipedia.org)
  • Mammalian ovaries are endowed with a huge number of small oocytes (primordial oocytes) in primordial follicles. (go.jp)
  • If the follicles are of adequate size and oocytes are mature, the physician will determine the timing of ovulation induction and the application of the medication necessary for inducing the final stage of egg maturation. (next-fertilitypilsen.cz)
  • Retrospective analysis to determine the size of follicles on day of trigger contributing most to the number of mature oocytes retrieved using generalized linear regression and random forest models applied to data from IVF cycles (2014-2017) in which either hCG, GnRHa, or kisspeptin trigger was used. (plandl.best)
  • Follicles 12-19 mm on the day of trigger contributed the most to the number of oocytes and mature oocytes retrieved. (plandl.best)
  • Using simulated follicle size profiles of patients with 20 follicles on the day of trigger, our model predicts that the number of oocytes retrieved would increase from a mean 9.8 (95% prediction limit 9.3-10.3) to 14.8 (95% prediction limit 13.3-16.3) oocytes due to the difference in follicle size profile alone. (plandl.best)
  • Follicles 12-19 mm on the morning of trigger administration were most likely to yield a mature oocyte following hCG, GnRHa, or kisspeptin. (plandl.best)
  • Once ovarian follicles grow to an appropriate size, a trigger is administered to mature the oocytes in preparation for oocyte retrieval. (plandl.best)
  • Importantly, knowledge of the size of follicles on day of trigger from which one could reasonably expect to retrieve a mature oocyte could enable the accurate determination of trigger efficacy. (plandl.best)
  • Oocyte retrieval is done without hospitalisation under analgosedation or a brief general anaesthesia by transvaginal puncture (vaginal route) of the ovaries under ultrasound control. (next-fertilitypilsen.cz)
  • Following the oocyte retrieval, the patient will stay under the supervision of the medical staff in the monitoring room. (next-fertilitypilsen.cz)
  • Oocyte retrieval from donor. (lushfertility.in)
  • Donor egg program includes oocyte retrieval from healthy young oocyte. (lushfertility.in)
  • In G-CSF group (n=30), 300 µg trans-cervical intrauterine of G-CSF was administered at the oocyte retrieval day. (peertechzpublications.com)
  • Mammalian oocytes are maintained in meiotic prophase arrest for a very long time-months in mice, years in humans. (wikipedia.org)
  • Therefore, in future, melatonin can be implicated as preferable therapeutic especially in IVF and assisted reproductive techniques. (jrhm.org)
  • In addition, other corrective measures include sperm separation for assisted reproductive techniques. (hindawi.com)
  • IUI is one of the simple and basic artificial reproductive techniques used in the treatment. (lushfertility.in)
  • This was a randomized controlled study performed at Assisted Reproductive Techniques Center of Ain Shams University Maternity Hospital, over a 2-year period, between Jan 2014 and Jan 2016, and included 60 infertile women with normal endometrial thickness. (peertechzpublications.com)
  • Despite major advancements in assisted reproductive techniques, the implantation rates remain relatively not high. (peertechzpublications.com)
  • Moreover, brief exposure during puberty may induce silent oocyte disruption, which could in turn lead to decreased fecundity rates. (biomedcentral.com)
  • The renin-angiotensin-aldosterone system (RAAS) is involved in maintaining blood volume and pressure whilst additionally playing a role in oocyte maturation and ovulation[5]. (eurorad.org)
  • Poor in vitro embryo development is a major obstacle in porcine assisted reproduction. (mdpi.com)
  • In the current study, we utilized modified Spirulina maxima pectin nanoparticles as a supplement to improve porcine in vitro maturation medium. (mdpi.com)
  • Adhesions - Scar-like tissue that abnormally attaches to internal organs, such as the fallopian tubes, ovaries, bladder, uterus or other internal organs. (nordicalagos.org)
  • The paired fallopian tubes extend laterally from the cornua of the uterus on each side and end near the ovaries. (medscape.com)
  • When the inability of male transgenic rats to transmit the transgene is not associated with mosaicism, microinsemination techniques such as intracytoplasmic sperm injection (ICSI) and round spermatid injection (ROSI) can be used for passage of the transgenes. (go.jp)
  • Our ICSI procedure includes the use of a piezo-driven small-sized injection pipette (2-4 μm in diameter), isolated sperm heads, and freshly ovulated oocytes. (go.jp)
  • These microinsemination techniques can be combined with cryopreservation of sperm and spermatid cells, leading to successful transmission of transgenes carried in male gametes. (go.jp)
  • Intracytoplasmic sperm injection (ICSI) is the most widely used technique of "artificial insemination", during which an egg is fertilised by inserting/microinjecting sperm directly into its cytoplasm. (next-fertilitypilsen.cz)
  • The technique was originally developed for couples with deteriorated spermiogram parameters, where the conventional fertilisation by adding sperm to eggs have failed, but thanks to the high success rate, this technique has now entirely replaced the conventional IVF method. (next-fertilitypilsen.cz)
  • in-vitro -matured oocytes were co-incubated with sperm according to a modified two-step culture system. (e-jarb.org)
  • In the first experiment, oocytes were briefly co-incubated with sperm, washed in IVF medium, and then moved to fresh IVF medium for 5 or 6 h. (e-jarb.org)
  • In experiment 2, oocytes were inseminated with sperm separated by Pecroll treatment. (e-jarb.org)
  • All treatments that involve combining the eggs that were removed from a woman's ovaries with sperm to enable pregnancy. (nordicalagos.org)
  • The eggs are extracted directly from the ovaries in our surgical suite under intravenous sedation administered by an anesthesiologist.The eggs are fertilized with sperm in the controlled environment of our lab. (drfernandoakerman.com)
  • in 2015 showed that supplementation of CoQ10 in an aged animal model delayed decline in ovarian reserve, restored oocyte mitochondrial gene expression, and improved mitochondrial activity with a significant reduction in oocyte aneuploidy. (arvigen.com)
  • The aim of this study was to compare the complication rates of oocyte pick-up (OPU) procedures via transvaginal ultrasonography in patients with different levels of ovarian reserve. (ecerm.org)
  • As a result, these aged mice had more oocytes with stimulation, the developmental potential of the oocytes was improved, and more offspring were born compared to old animals receiving placebo 1. (arvigen.com)
  • Female age, ASRM stage, postoperative to IVF duration, stimulation protocol, AFC, AMH, number of oocytes retrieved, number of high-quality cleavage embryos and number of transferred embryos are independent influencing factors for the clinical pregnancy rate in individuals with endometriosis receiving fresh embryo transfer. (biomedcentral.com)
  • The principle of the IVF/ICSI cycle is a controlled hormonal stimulation of the patient's ovaries, which will allow us to obtain a higher number of fertile eggs from both ovaries, compared to the natural cycle when only one oocyte matures. (next-fertilitypilsen.cz)
  • To achieve this, cells of in vitro produced bovine embryos were traced from the 2-cell stage to the blastocyst stage. (bioone.org)
  • Higher percentage of MII stage oocytes in experiment 1(65.2±2.0), embryos at morula stage in experiment 2 (30.4±1.5) and experiment 3 (22.2±2.0) were obtained. (bvsalud.org)
  • In this paper, we describe a scanning electrochemical microscopy (SECM) technique that is a non-invasive and sensitive method for measuring oxygen consumption by individual embryos. (bioone.org)
  • Along with polyspermy, the suboptimal quality of IVF-derived pig embryos poses another challenge that needs to be addressed for in vitro production systems. (e-jarb.org)
  • Successful implantation requires good quality of embryos, receptive endometrium, and proper embryo transfer technique" [1]. (peertechzpublications.com)
  • The aim of this study was to assess the effects of brief, intense exposure to tobacco smoke on granulosa cell number, oocyte growth, and follicle size during puberty in female Swiss mice. (biomedcentral.com)
  • However, limited data exist to establish which follicle size on the day of trigger is most likely to yield a mature oocyte. (plandl.best)
  • The decreased quality of oocytes involves an increased rate of chromosomal aneuploidy with aging predominantly related to meiotic errors during oocyte maturation. (arvigen.com)
  • However, as the oocyte grows, these proteins are synthesized, and meiotic arrest becomes dependent on cyclic AMP . (wikipedia.org)
  • Maintenance of meiotic arrest also depends on the presence of a multilayered complex of cells, known as a follicle, that surrounds the oocyte. (wikipedia.org)
  • In the oocyte, cyclic GMP prevents the breakdown of cyclic AMP by the phosphodiesterase PDE3, and thus maintains meiotic arrest. (wikipedia.org)
  • The current pregnancy was initiated with assisted fertility in the form of in-vitro fertilisation (IVF). (eurorad.org)
  • 2019: Daniela Rodler (Department of Veterinary Sciences, Ludwig-Maximilian University of Munich, Munich, Germany) for the paper entitled: "Localization of thrombospondin-1 and its receptor CD36 in the ovary of the ostrich (Struthio camelus)" in co-authorship with F. Sinowatz and published in the Journal Anatomia Histologia Embryologia 2018, 47:124-132. (wava-amav.org)
  • [5] Because the granulosa cells and oocyte are connected by gap junctions, cyclic GMP also decreases in the oocyte, causing meiosis to resume. (wikipedia.org)
  • Cycle fecundability decreases as the number of oocytes decreases. (medscape.com)
  • The number of oocytes decreases to approximately 2-3 million at birth and decreases again to 300,000 by the time of puberty. (medscape.com)
  • Diagram showing the reduction in number of the chromosomes in the process of maturation of the ovum . (wikipedia.org)
  • oocytogenesis , ootidogenesis , and finally maturation to form an ovum (oogenesis proper). (wikipedia.org)
  • Even if a patient's reserve is very good, fewer and higher-quality oocytes should be targeted with the use of the lowest possible dose of drugs. (ecerm.org)
  • Construction of an artificial ovary could be the optimal way of preserving fertility as well as protecting female cancer patients, against the re-transplantation of malignant cells, independent from type of neoplastic diseases [ 9 ]. (biomedcentral.com)
  • IUI - D (donor) - is an ART (artificial reproductive technique) procedure where in the. (lushfertility.in)
  • Fertility preservation is the technique of conserving or storing one's. (lushfertility.in)
  • A cycle begins when a woman begins taking fertility drugs or having her ovaries monitored for follicle production. (nordicalagos.org)
  • This review focuses on the differential responses of cell types to ATZ toxicity, testicular effects of ATZ in both in vitro and in vivo models and chemopreventive strategies, so as to highlight the current state of the art on the toxicological outcomes of ATZ exposure in several experimental model systems. (frontiersin.org)
  • Oogenesis starts with the process of developing primary oocytes, which occurs via the transformation of oogonia into primary oocytes , a process called oocytogenesis . (wikipedia.org)
  • The decline in the number of oocytes begins at 20 weeks' gestation when the female fetus has approximately 6-7 million oogonia (largest lifetime endowment). (medscape.com)
  • Oocyte meiosis, important to all animal life cycles yet unlike all other instances of animal cell division, occurs completely without the aid of spindle -coordinating centrosomes . (wikipedia.org)
  • Oocyte pick-up (OPU) via transvaginal ultrasonography is a commonly used oocyte collection method that is less invasive than other methods [ 4 ]. (ecerm.org)
  • The large volume of medium significantly reduced the number of dead oocytes and increased the rate of blastocyst formation compared to the small volume. (e-jarb.org)
  • The fact that live-birth rates from oocyte donation in older women are consistent with the age of the donor suggests that oocyte quality is the major factor responsible for reduced fecundability with aging. (arvigen.com)
  • When we have a patient in need of a donor egg/ oocyte, we chose a suitable one from the Donor bank after matching the profile of the lady in need of the donor eggs with the donor. (lushfertility.in)
  • The length of time between admission into the pool and an egg donation can be just a couple of weeks or one year or more depending on the donor's characteristics and the type of donor the recipient couple are searching for. (drfernandoakerman.com)
  • In this review, we summarize the results regarding the activation of primordial oocytes in neonatal and adult ovaries of different species and propose a model in which ovaries of neonatal mammals contain a mixed population of both quiescent and activated primordial oocytes, while almost all primordial oocytes are quiescent in adult females. (go.jp)
  • These quiescent primordial oocytes are activated, perhaps by certain mechanisms involving the interaction between stimulatory and inhibitory factors, to enter the growth phase. (go.jp)
  • This experiment was conducted to examine the effects of using different forms and levels of Echinacea purpurea L. (EP) in comparison with an antibiotic growth promoter (flavophospholipol) on performance, carcass characteristics and immune responses of broiler chicks. (researchgate.net)
  • When in vitro experimental models are exposed to ATZ, they are faced with increased levels of reactive oxygen species (ROS), cytotoxicity and decreased growth rate at dosages that may vary with cell types. (frontiersin.org)
  • A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
  • Abstract The objective of the current study was to investigate the synergistic impact of -Tocopherol and -Linolenic acid (100 µM) on IVM and IVC of Nili Ravi buffalo oocytes. (bvsalud.org)
  • Apart from pineal, several extra pineal sites of melatonin biosynthesis in mammals have been identified including skin, retina, gut, ovary, and testis. (jrhm.org)
  • We think that primordial oocytes in adult mammals are different from those in neonatal mammals. (go.jp)
  • The dormancy of primordial oocytes may be required to reserve the non-growing oocyte pool for the long reproductive life in mammals. (go.jp)
  • After the onset of puberty and menses, the female human ovary recruits at least 30-50 oocytes during each menstrual cycle. (medscape.com)
  • Supplementation of -tocopherol in maturation media having -Linolenic acid and/or in embryo culture media did not further enhance in vitro maturation of oocyte or embryo production. (bvsalud.org)
  • However, this procedure runs the risk of re-transplanting of malignant cells, which makes it unsuitable for patients suffering from neoplastic diseases whose malignant cells are likely to metastasize into the ovary. (biomedcentral.com)
  • Typical characteristics include a cyst that is 2-10cm in diameter with peripheral vascularity and a diffusely thickened and crenulated wall[6]. (eurorad.org)
  • In the case of ROSI, round spermatids are injected into Sr 2+ -activated oocytes using a piezo-driven middle-sized pipette (5-6 μm in diameter). (go.jp)
  • To identify follicle sizes on the day of trigger most likely to yield a mature oocyte following hCG, GnRH agonist (GnRHa), or kisspeptin during IVF treatment. (plandl.best)
  • Cumulus expansion and nuclear maturation status was determined (Experiment 1). (bvsalud.org)
  • In experiment 2, oocytes were matured as in experiment 1. (bvsalud.org)
  • In experiment 3, fertilized oocytes were cultured in 25 μL microdroplets (10 gametes/drop) or 500 μL (100 gametes/well) of culture medium in 4-well plates. (e-jarb.org)
  • Woodruff returned to Northwestern University as a faculty member in 1995 to study inhibin and activin actions and interactions within the pituitary-gonadal axis, characterizing the regulation of subunit assembly and ligand processing in the ovary, the ligands' role in paracrine regulation of folliculogenesis, and their signal transduction pathways in the regulation of follicle-stimulating hormone (FSH). (wikipedia.org)
  • The discrimination, accuracy, and clinical efficacy of the prediction model were analyzed utilizing the receiver operating characteristic (ROC) curve, calibration curve, and clinical decision curve. (biomedcentral.com)
  • A characteristic pathological manifestation of endometriosis is the occurrence of endometrial tissue beyond the confines of the uterine cavity. (biomedcentral.com)
  • Dr. Akerman offer the most advanced surgical techniques with particular expertise in reconstructive surgery for the treatment of tubal disease, endometriosis, myomas, and reversal of tubal ligation surgery. (drfernandoakerman.com)
  • The characteristic MRI appearance of a reproductive female ovary is a homogenous soft-tissue T1-weighted signal intensity, which can be difficult to differentiate from adjacent bowel. (eurorad.org)