• 2016). Thus, mice lacking B cells had less TFH cells and an increase Tedalinab in TRM cells, explaining why B cell deficient mice had increased airway inflammation when challenged with HDM inhalation long after the priming period (Hondowicz et al. (icem2012.org)
  • The present study examined the therapeutic effects and action mechanism of a saponin-enriched extract of Asparagus cochinchinensis (SEAC) on airway inflammation and remodeling in an ovalbumin (OVA)-induced asthma model. (spandidos-publications.com)
  • Cysteine (CYSH), glutathione (GSH), and markers of inflammation in bronchoalveolar lavage fluid (BALF) were measured following ovalbumin (OVA) inhalation challenge. (cdc.gov)
  • In order to determine whether adoptive transfer of induced Treg cells generated in vitro can be used as an effective therapeutic approach to suppress airway allergic inflammation, exogenously induced Treg cells were infused into ovalbumin-sensitized mice prior to or during intranasal ovalbumin challenge. (ca.gov)
  • Moreover, adoptive transfer of induced Treg cells during allergen challenge also effectively attenuate airway inflammation and improve airway function, which are comparable to those by natural Treg cell infusion. (ca.gov)
  • While the results of the present study do not support a functional role for thiols in the immune response, it is important to note the dose-dependent dramatic alteration seen in thiols following sensitization and challenge. (cdc.gov)
  • Total cell count, tissue TNF- α, IL -33, and serum IgE increased considerably after sensitization to ovalbumin (OVA), while GSH levels decreased significantly. (edu.iq)
  • Sensitization and Challenge Sensitization was carried out by intraperitoneal shot of 20 μg OVA (Sigma. (siamtech.net)
  • BALB/c mice without and with ovalbumin sensitization/challenge were infected with candidate bacteria by oral gauge intra-gastric intubation. (researcher-app.com)
  • 001). In mice, endogenous R gnavus grew rapidly following sensitization and challenge with ovalbumin. (researcher-app.com)
  • The aim of this study was to assess how exposure to fine PM collected from Sacramento, CA, shapes the allergic airway immune response in BALB/c mice undergoing sensitization and challenge with ovalbumin (OVA). (cdc.gov)
  • Allergic asthma was induced in female BALB/c mice by intraperitoneal administration of ovalbumin (OVA) on days 0 and 14, followed by intratracheal challenge with 1% OVA six times between days 22 and 30. (blogspot.com)
  • BALB/c mice were sensitized and challenged with OVA. (kyobobook.co.kr)
  • Male BALB/c mice were actively sensitized with ovalbumin antigen, and were repeatedly challenged with aerosolized antigen. (openrespiratorymedicinejournal.com)
  • Since chicken ovalbumin (OVA) is one of the most abundant proteins consumed by people worldwide and is a generally recognized as a safe (GRAS) protein, HP-modified OVA has great potential for further development as an effective, safe and affordable microbicide. (biomedcentral.com)
  • We found that the expression of foreign antigens such as chicken ovalbumin (OVA) and green fluorescent protein (GFP) in Stat1-competent 291 cells led to immune responses that delayed tumor progression and improved survival of wild-type animals. (uni-muenchen.de)
  • To this end, rats were actively sensitized to ovalbumin (OVA) and submitted or not to OVA challenge to assess the development of smooth muscle hyperreactivity. (ndltd.org)
  • Daqing formula ameliorated allergic asthma and airway dysbacteriosis in mice challenged with ovalbumin and ampicillin. (bvsalud.org)
  • METHODS AND MATERIALS The mice were co-challenged with ovalbumin and ampicillin to induce allergic asthma combined with airway dysbacteriosis . (bvsalud.org)
  • In the present study, we investigated the possible protective effect of cinnamic acid (CA) on ovalbumin-induced asthma in a mouse model. (edu.iq)
  • Study the Anti-Asthmatic Activity of Guggulsterone in Ovalbumin-Induced Asthma in Rat. (edu.iq)
  • Kim DI, Song MK, Lee K. Comparison of asthma phenotypes in OVA-induced mice challenged via inhaled and intranasal routes. (edu.iq)
  • The 'classical' ovalbumin challenge model of asthma in mice. (edu.iq)
  • Asthma was induced by challenge and repeated exposure to ovalbumin. (waldlog.com)
  • Micro- array data from the ovalbumin and TMA model of asthma were also compared to previous data using Aspergillus as allergen to identify putative asthma `signature genes', i.e. genes up-regulated with all 3 allergens. (cdc.gov)
  • Examples of high molecular environmental factors are known to influence the development weight occupational allergens include proteases used in the and expression of asthma, and a vast array of triggers of asthma detergent industry, laboratory animal allergens, and ovalbumin have been identified. (cdc.gov)
  • The degree of the hyperresponsiveness measured by methacholine challenge correlates with the severity of asthma. (indexarticles.com)
  • Ammit and colleagues [ 7 ] firstly demonstrated that S1P levels are elevated in the airways of individuals with asthma after segmental allergen challenge. (openrespiratorymedicinejournal.com)
  • Animals also received an intranasal administration of S1P (10-5 M, 20 µL) or its vehicle (1% methanol in sterile PBS, 20 µL) 30 min prior to each antigen challenge. (openrespiratorymedicinejournal.com)
  • Immune function was assessed using a vaccine challenge with ovalbumin (OVA) and equine influenza as antigens during week 22 to 29. (uky.edu)
  • Inhalation of RS - and R -salbutamol 30 min before and 5.5 h after allergen challenge suppressed allergen-induced airway hyperreactivity to histamine after the early and late asthmatic reaction, completely inhibiting the early asthmatic reaction and tending to reduce the development of the late asthmatic reaction. (ersjournals.com)
  • After 14 days, repeated allergen inhalation challenges were performed in the sensitized rats for 2 weeks. (indexarticles.com)
  • During week 28 to 35 immune function was evaluated using a vaccine challenge with keyhole limpet hemocyanin (KLH) and equine influenza as antigens. (uky.edu)
  • The infiltration of immune cells, such as Th2 cytokines in the lungs, and the lung histology, were assessed in the OVA-challenged mice in the presence and absence of an intratracheal administration of bvPLA2. (blogspot.com)
  • To elucidate whether allergic bronchitis interferes with vitamin A metabolism, we measured the vitamin A levels in the serum, the lung, and the liver following repeated allergen challenge in a rat model and compared them to normal rats. (indexarticles.com)
  • Dose-dependent thiol and immune responses to ovalbumin challenge in Brown Norway rats. (cdc.gov)
  • OVA challenge to sensitized rats induced increases in BALF albumin, total protein, lactate dehydrogenase, CYSH and GSH that were independent of serum antibody concentrations. (cdc.gov)
  • CYSH, but not GSH, was elevated in LNC of OVA challenged rats. (cdc.gov)
  • In summary, antigen exposure caused a dose-dependent alteration of inflammatory, thiol and immune parameters in OVA sensitized and challenged rats. (cdc.gov)
  • Stimulated with increasing concentrations of acetylcholine and KCl, rats tracheal rings challenged with OVA was contracted with significantly higher magnitude compared to the non-challenged group. (ndltd.org)
  • On day 16, allergen challenge was performed and bronchoconstriction was measured in all 24 rats. (indexarticles.com)
  • Results: The study rats showed severe bronchoconstriction after allergen challenge compared to the naive rats, and ex vivo TNF-[alpha] and NO production was significantly higher in the sensitized rats. (indexarticles.com)
  • Added directly into the bath chamber containing Ca2+ in free form, EPJP and its main constituent, coumarin, preferably inhibited concentration-effect curves induced by increasing concentrations of KCl or acetylcholine in rat tissues challenged with OVA, compared to those curves made for Ca2+, in the presence of the same agonists in assays performed in medium without Ca2+. (ndltd.org)
  • Four weeks after pneumonia, mice were sensitized and challenged with OVA to induce allergic airway disease (AAD). (nature.com)
  • Surprisingly, we found that a very short period of exposure to ovalbumin was sufficient to elicit early changes of remodeling. (monash.edu)
  • Since route of allergen exposure can influence mechanistic pathways, mice were sensitized by identical routes with a high molecular weight occupational allergen ovalbumin and a low molecular weight occupational allergen trimellitic anhydride (TMA). (cdc.gov)
  • Moreover, male mice orally challenged with 1 mg OVA, and all mice orally challenged with 40 mg OVA were severely hypothermic post OVA challenge, while mice orally challenged with 1 mg OVA did not display any clinical symptoms of allergy post challenge. (usda.gov)
  • The development of the nanoparticle addresses the challenge of dispensing a vaccine through the lungs via an aerosol spray without activating an immune response that neutralizes it. (quantumday.com)
  • The immune adjuvant effect of olive, soybean and corn oils in Swiss mice immunized with ovalbumin (OVA) plus aluminum hydroxide or emulsified in Marcol, soybean, olive or corn oils was evaluated through the OVA-specific antibodies determined by ELISA and Passive Cutaneous Anaphylaxis. (sld.cu)
  • The effect of antigen challenge on the function of neuronal M2-muscarinic autoreceptors in the lungs was studied in anesthetized guinea pigs. (nih.gov)
  • In order to assess the specificity of MICCop-induced suppression, treated EAE mice were challenged with the control protein ovalbumin. (uni-koeln.de)
  • Therefore the aim of this project was to identify the lowest egg allergen ovalbumin (OVA) dose causing hypersensitization using a validated murine model. (usda.gov)
  • Here we demonstrate that Cd44v7 -deficient T cells - like Cd44 wild-type ( Cd44 WT ) T cells - provoked disease in two different colitis models: the model induced by CD4 + CD45RB high T-cell transfer into Rag2 -deficient mice and a new model based on ovalbumin (OVA)-specific T-cell transfer into Rag -sufficient, OVA-challenged mice. (nature.com)
  • Continued studies focused on genes showing minimal changes in the TMA-induced model but with clear up-regulation in the ovalbumin model. (cdc.gov)
  • Objective: To examine whether repeated allergen challenges could increase vitamin A consumption in a rat model. (indexarticles.com)
  • The results showed that adoptive transfer of induced Treg cells prior to allergen challenge markedly reduced airway hyperresponsiveness, eosinophil recruitment, mucus hyper-production, airway remodeling, and IgE levels. (ca.gov)
  • Seventy-two hours after the final challenge the airway pressure time index (APTI) which steps airway hyperresponsiveness was recorded. (monossabios.com)
  • At the time of challenge, serum vitamin A levels for the control and A-deficient (A-def) lambs were 33.3 and 3.1 ug·dl-1 respectively. (vt.edu)
  • In chronically challenged mice, these changes persisted and, in addition, subepithelial collagen deposition was significantly increased. (monash.edu)
  • The hyperresponsive phenotype of the challenged tissue decreased significantly after oral treatment with EPJP. (ndltd.org)
  • The Ii-OVA vector was the most potent inducer of IFN-γ-secreting CD4 + and CD8 + T cells and was the only vector to protect mice completely from challenge with OVA-expressing tumor cells. (elsevierpure.com)
  • We examined the sequence of events in remodeling using three commonly used mouse models of allergic airways disease in which mice are exposed to nebulized ovalbumin for four consecutive days (acute), seven consecutive days (subacute), or three times a week for 6 wk (chronic). (monash.edu)
  • Guinea pigs were injected intraperitoneally with saline (control group) or ovalbumin (10 mg/kg) on days 1, 3, and 5. (nih.gov)
  • At 5 h after allergen challenge, the inhibition of airway hyperreactivity was more pronounced in animals treated with R -salbutamol compared to racemate-treated animals. (ersjournals.com)
  • We selected maleic anhydride-modified ovalbumin (ML-OVA) for further study because OVA is easier to obtain than β-LG, and ML is safer than HP. (biomedcentral.com)
  • Post-oral challenge, mice were euthanized and blood samples were analyzed for plasma IgE levels. (usda.gov)
  • Male mice orally challenged with 1 mg OVA had approximately a 3 fold increase in plasma OVA IgE levels compared to female mice orally challenged with 1 mg OVA. (usda.gov)
  • Here, we show that after oral ovalbumin (OVA) challenge, allergic mice present higher levels of anxiety, increased Fos expression in emotionality-related brain areas, and aversion to OVA-containing solution. (fabresearch.org)
  • One group of sensitized animals was challenged on days 20-25 with aerosolized ovalbumin for 5 min/day (challenged group), while another group of the sensitized animals was not challenged (sensitized group). (nih.gov)
  • There was no increase in baseline Ppi in the sensitized or challenged animals compared with the controls. (nih.gov)
  • However, relapse after conventional chemotherapy is an important challenge, especially in high grade B cell lymphomas. (uni-muenchen.de)
  • Conclusions: We conclude that vitamin A utilization is increased during repeated allergen challenge and allergic bronchitis, most probably due to increased demand for epithelial repair. (indexarticles.com)
  • AM thiols were modestly elevated at low OVA challenge doses, but sharply reduced at the higher OVA challenge doses. (cdc.gov)