• screening a sample such as water for bacteria, fungi, and parasites. (bio.net)
  • In comparison, MALDI-TOF-MS can identify bacteria and fungi within minutes directly from colonies grown on culture plates. (molvis.org)
  • One of the most widely used and promising technology to break down the pollutants is bioremediation, which uses selected microorganisms such as fungi, yeast, and bacteria [ 4 ]. (omicsonline.org)
  • Microorganisms capable of catabolizing lignin-derived ar- carbohydrate and protein components of the organic matter omatics are essentially fungi and bacteria (Bugg et al. (lu.se)
  • A safe bio-preservative agent, lactic acid bacteria (LAB) can inhibit the growth of pathogenic bacteria and spoilage organisms. (frontiersin.org)
  • However, there have not been many investigations on how pathogenic bacteria are affected by the metabolic properties of planktonic and biofilm probiotics. (frontiersin.org)
  • Ingestion of pathogenic bacteria in foods often results in illnesses that are of worldwide concern. (usda.gov)
  • Cellular organelles isolated by differential centrifugation can maintain an incredibly high level of normal function, so long as they're not exposed to denaturing conditions in isolation. (microbiologynote.com)
  • The isolation of organelles in sub-cellular form is a fundamental procedure in numerous biochemical research labs through this use of different centrifugation methods. (microbiologynote.com)
  • Because lysis-centrifugation blood cultures show enhanced isolation of B henselae and B quintana, intracellular forms are most likely present in humans. (medscape.com)
  • The secretory pathway translocates unfolded proteins from the reducing cytoplasm into the endoplasmic reticulum lumen in yeast or into the periplasm in bacteria. (jove.com)
  • For the purpose of this study, isolated bacteria from the biofloc sample were identified as bioflocculant-producing bacteria using yeast peptone glucose agar as it shows highly mucoid and ropy colonies morphology. (scialert.net)
  • MVs are released from the outer membrane of bacterial cells, such as outer membrane vesicles (OMVs) from Gram-negative bacteria, and from the cytoplasmic membrane of cells, such as MVs from Gram-positive bacteria. (nature.com)
  • 6. Remove most of the bacterial solution, seal the Petri dishes with Parafilm, and co-culture the bacteria and root bundles for 40-50 hours in a growth chamber at 20 o C. (purdue.edu)
  • In other particular aspects, the bacterial cell is an Enterobacter or Serratia bacteria. (justia.com)
  • Various pigments like carotenes, melanins, flavones, quinines and violacein[ 4 - 6 ] are produced by different bacterial genera like Serratia , Streptomyces , Pseudomonas , Bacillus , Vibrio and Cytophyga that play an important role in their molecular and physiological processes and help taxonomist to identify and classify the bacteria, as they differ in chemical structure with specific chromophores. (ijpsonline.com)
  • However, much is unknown about the effects of indigenous bacteria on microalgal growth and the characteristics of bacterial communities associated with microalgae in microalgae-effluent culture. (biomedcentral.com)
  • To enhance their own growth, microalgae may be able to selectively stimulate specific bacterial groups from among the in situ indigenous bacterial community found in wastewater effluent (i.e., microalgae growth-promoting bacteria: MGPB). (biomedcentral.com)
  • Twelve tickborne bacterial species were detected, including two novel pathogens, representing a 100% increase in the number of tickborne bacteria identified compared to what was possible by initial PCR testing. (cdc.gov)
  • Abstract Starting from mature vegetable compost, four bac- the utilization of these bacteria as cell factories for upgrading terial strains were selected using a lignin-rich medium. (lu.se)
  • Gram staining classifies bacteria as positive (purple) or negative (pink) according to the cell wall structure and chemical composition. (bvsalud.org)
  • Use sealed centrifuge rotors or sample cups, if available, for centrifugation. (cdc.gov)
  • The goal for this scenario is for the user to select the correct tubes, properly balance, and load the centrifuge during the centrifugation process. (cdc.gov)
  • Streptococcus pneumoniae is a gram-positive bacterium cephalosporins as high as 32 µg/mL(8,9). (cdc.gov)
  • Recently, some specific Gram-positive bacteria, including Mycobacterium tuberculosis and Corynebacterium glutamicum , were found to possess a novel cell wall structure consisting of an arabinogalactan layer covalently linked to the peptidoglycan layer, and a special outer membrane consisting of mycolic acids (mycomembrane) [ 19 ]. (nature.com)
  • The homogenized vitreous samples, with or without centrifugation, were stained with Gram and Coomassie Blue (CBB) dyes and cultured with blood agar. (molvis.org)
  • Whooping cough, also known as pertussis, is a respiratory disease that is predominantly caused by the gram-negative bacterium Bordetella pertussis , which is found only in humans [ 1 ]. (biomedcentral.com)
  • This study focused on the changes in biofilm activity and related metabolic pathways of S. aureus treated with lactic acid bacteria planktonic CFS (LAB-pk-CFS) and biofilm state (LAB-bf-CFS). (frontiersin.org)
  • Here, we used untargeted metabolomics to investigate the potential impacts of planktonic bacteria (LAB-pk-CFS) and biofilm colonies (LAB-bf-CFS) on the metabolism of S. aureus biofilms. (frontiersin.org)
  • Two physical methods (centrifugation and ultrasonication) and 3 chemical methods (extraction with EDTA, extraction with formaldehyde, and extraction with formaldehyde plus NaOH) for extraction of EPS from alga-bacteria biofilm were assessed. (scielo.org.za)
  • We retrospectively investigated the bacteria from endophthalmitis samples (aqueous humor and vitreous) and found that the three organisms most commonly responsible are Staphylococcus spp . (molvis.org)
  • Bacteria are typically found as communities made up of several different species rather than as isolated, solitary organisms in nature ( 6 ). (frontiersin.org)
  • Although these organisms were originally thought to be rickettsiae, Bartonella bacteria can be grown on artificial media, unlike rickettsiae. (medscape.com)
  • Disinfection - Water is treated with a chemical disinfectant such as chlorine, chlorine dioxide, or chloramines to kill any remaining bacteria, parasites, or viruses. (lakeside-equipment.com)
  • Because microscopic detection usually requires a microbe concentration of at least about 1 × 10 4-5 /mL, most body fluid specimens (eg, cerebrospinal fluid) are concentrated (eg, by centrifugation) before examination. (msdmanuals.com)
  • In three blood specimens, two tickborne bacteria were simultaneously detected. (cdc.gov)
  • PAHs could be degraded by bacteria under aerobic conditions through the initial oxidation of the aromatic ring, which is catalyzed by the dioxygenase enzyme. (omicsonline.org)
  • Microscopic examination of unstained feces was performed after centrifugation in a zinc sulfate solution, thin fecal smears were stained with acid fast stain and examined for C parvum , and bacteriologic culture of feces was used to detect aerobic and anaerobic bacteria. (avma.org)
  • In a typical situation that differential centrifugation is utilized to study cell-biological processes (e.g. organelle distribution) A tissue specimen is very first de-lysed in order to cut the cell membranes, and let out organelles and the cytosol. (microbiologynote.com)
  • More particles settle down based on the speed and length of the individual centrifugation processes as well as the density and relative sizes of each particle. (microbiologynote.com)
  • 2007 ). Strongest interest is focused on understanding what factors cause variations in microbiota composition and how these gut bacteria modulate host health (Louis et al. (springer.com)
  • 2007 ). Our work aims to stimulate the growth of health-promoting probiotic gut bacteria by using newly synthesized non-digestible carbohydrates, i.e., prebiotic compounds. (springer.com)
  • They are fermented by various commensal and health-beneficial gut bacteria, thus promoting their growth and activity which may confer health benefits upon the host (Roberfroid et al. (springer.com)
  • To assess the effects of the indigenous bacteria in wastewater effluent on microalgal growth, three microalgae, Chlamydomonas reinhardtii , Chlorella vulgaris , and Euglena gracilis , were cultured in two municipal wastewater effluents and one swine wastewater effluent with and without indigenous bacteria for 7 days. (biomedcentral.com)
  • It's also not as effective as reverse osmosis and removes about 90% of salts and almost all of the bacteria, organic matter, and viruses in water. (lakeside-equipment.com)
  • Hence, our research efforts have focused on developing screening tests capable of multiplexed detection of foodborne bacteria and associated toxins. (usda.gov)
  • Immobilized bacteria were labeled with a fluorescent dye-antibody conjugate to allow for quantitative detection of the antibody captured bacteria using laser-induced fluorescence scanning. (usda.gov)
  • This new strategy could be used for other bacteria and represents a new rapid method for detection of clinical antibiotic resistance. (bvsalud.org)
  • Differential centrifugation is a good choice for the separation of crude particles just on the sedimentation rate However, more fine-grained purifications can be made using density by using equilibrium centrifugation using density gradients. (microbiologynote.com)
  • Intracellular membrane structures are rare in bacteria, thus making them ideal chasses for cell-based vesicle production. (biomedcentral.com)
  • Differential centrifugation (also called the differential velocity method) is a common process in the fields of biochemistry and cell biology it is utilized to differentiate organelles and other subcellular particles by their sedimentation rates. (microbiologynote.com)
  • Thirty halophilic bacteria were isolated from the soil of Karwar mangrove regions, Karnataka. (ijpsonline.com)
  • From six species of bioflocculant-producing bacteria [( Bacillus infantis (B. infantis ), Bacillus cereus ( B. cereus ), Bacillus safensis ( B. safensis ), Halomonas venusta ( H. venusta ), Nitratireductor aquimarinus ( N. aquimarinus ) and Pseudoalteromonas ], there was 64 consortium of bioflocculant-producing bacteria were produced using the sum of combination calculation. (scialert.net)
  • The most consortium that has more than 80% flocculation activity derived from consortiums of single and combination of two bioflocculant-producing bacteria species. (scialert.net)
  • Among several sample reaction conditions (e.g., static vs. agitation) that were compared, centrifugation was the only one found to significantly improve localization of bacteria (pathogenic E. coli O157:H7) to planar capture surfaces containing the printed antibodies. (usda.gov)
  • Alterations due to co-cultivation with bacteria could not only be observed in fungal morphology but also in fungal transcriptome. (frontiersin.org)
  • and show that these bacteria could serve in plant protection. (frontiersin.org)
  • Moreover, these infections the survival of Yersinia and other bacteria show a modest predilection for males, with in cold enrichment. (who.int)
  • For certain experiments, you may wish to resuspend the bacteria at a Klett of 100, or a Klett of 1 or even less. (purdue.edu)
  • Each of these prebiotic compounds may exert specific and selective effects on gut bacteria. (springer.com)
  • Microalgal productivity could be enhanced if the positive effects of indigenous bacteria could be exploited. (biomedcentral.com)
  • Owing in part to the inability of phages to attach to spores, seed banks stabilized population dynamics and resulted in minimum host densities that were 30-fold higher compared to bacteria that were unable to engage in dormancy. (nature.com)
  • When the inoculated number was 1.0 × 10 4 CFU/μl or higher, the bacteria in the vitreous pellets could be identified in all samples (100%, n = 9). (molvis.org)
  • The RNeasy Protect Mini Kit and Bacteria Mini Kit include RNeasy spin columns for purifying up to 100 µg of high-quality RNA using silica-membrane technology. (qiagen.com)
  • This study was aimed to test and applied bioflocculant-producing bacteria isolated from biofloc for flocculation process potentially used as inoculum for rapid formation of biofloc. (scialert.net)
  • Bacteria among all the microbes have immense potential to produce diverse pigments. (ijpsonline.com)
  • A select agent is a bacterium, virus or toxin that has the potential to pose a severe threat to public health and safety. (cdc.gov)
  • Treatment of the roots with bacteria prior to infection with V. dahliae resulted in a significant reduction of fungal root colonization. (frontiersin.org)
  • Plant infection begins at the roots, where the fungus is confronted with rhizosphere inhabiting bacteria. (frontiersin.org)
  • In the vitreous samples inoculated with S. aureus and cultured for 12 h, the number of S. aureus increased in a dose-dependent manner to the number of bacteria in the inoculate. (molvis.org)
  • Bacteria have been studied extensively, and there is information regarding their ability to degrade xenobitics, including PAHs [ 6 ]. (omicsonline.org)