Loading...
  • senescence
  • In recent years, researchers have provided evidence to suggest that cellular senescence mechanisms are partly involved in the decline of stem cell activity in aging. (fightaging.org)
  • upon regenerative or proliferative pressure, these cells undergo accelerated senescence ( geroconversion ), through Rb -mediated repression of E2F target genes . (fightaging.org)
  • Thus, p16INK4a/Rb-driven stem cell senescence is causally implicated in the intrinsic defective regeneration of sarcopenic muscle. (fightaging.org)
  • Here we discuss on how cellular senescence may be a common mechanism of stem cell aging at the organism level and show that induction of p16INK4a in young muscle stem cells through deletion of the Polycomb complex protein Bmi1 recapitulates the geriatric phenotype . (fightaging.org)
  • With the newfound and rapidly spreading interest in senescent cells in the research community over the past few years, a lot of efforts are now underway to better understand how cellular senescence fits into the existing knowledge of the biochemistry of age-related conditions. (fightaging.org)
  • The expression of senescence-associated markers p21 and p16ink4a and the proportion of SA-β-gal positive cells increased with age. (fightaging.org)
  • Senescence and death of CSCs with increasing age in wild type mice has been implicated in impairment of growth and turnover of cells in the heart. (fightaging.org)
  • therapies
  • Using a human HD patient model of the central nervous system, hopefully one day researchers can tease out the consequences of mutant HTT (mHTT) expression on specific cell types within the brain in order to identify and test novel therapies for disease. (springer.com)
  • Following the success of the 1st and 2nd International Congress on Controversies in Stem Cell and Cellular Therapies (COSTEM), we are honored to invite you to the 3rd COSTEM Congress to be held in Berlin, Germany on October 22-24, 2015. (tsnn.com)
  • Aiming to integrate expanses of information with its implementation in best clinical care, this state-of-the-art Congress is unique in its focus to resolving vital controversies in Stem Cell and Cellular Therapies. (tsnn.com)
  • Human iPS cells - which have the ability to differentiate into any type of specialized cell - may be able to transform transplantation medicine by creating patient-specific cell-replacement therapies to treat neurological diseases, heart ailments, blood diseases and diabetes. (uab.edu)
  • This study moves research a step further towards the development of blood regeneration therapies, but the researchers believe it is also applicable to a variety of cells and will yield insights about regenerating diseased or damaged tissues. (eurekalert.org)
  • bone
  • Low/Negative Expression of PDGFR-α Identifies the Candidate Primary Mesenchymal Stromal Cells in Adult Human Bone Marrow. (lu.se)
  • By tagging bone marrow cells of mice with a genetic label, or barcode, researchers were able to track and describe the family tree of individual blood cells as they form in their natural environment. (eurekalert.org)
  • A. Khojasteh and S. R. Motamedian, "Mesenchymal stem cell therapy for treatment of craniofacial bone defects: 10 years of experience," Regeneration, Reconstruction & Restoration , vol. 1, no. 1, p. 1, 2016. (hindawi.com)
  • MSC are unique bone marrow-derived multipotent progenitor cells that are presently being exploited as gene therapy vectors for a variety of conditions, including cancer and autoimmune diseases. (frontiersin.org)
  • Granulocyte-colony stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein that stimulates the bone marrow to produce granulocytes and stem cells and release them into the bloodstream. (wikipedia.org)
  • The stem cells are collected from donor's blood through a process known as apheresis after a certain period of daily subcutaneous injections of Granulocyte-colony stimulating factor, serving to mobilize stem cells from the donor's bone marrow into the peripheral circulation. (wikipedia.org)
  • Haematopoietic stem cells (HSCs) reside in the medulla of the bone (bone marrow) and have the unique ability to give rise to all of the different mature blood cell types and tissues. (wikipedia.org)
  • When bone marrow develops, it eventually assumes the task of forming most of the blood cells for the entire organism. (wikipedia.org)
  • Although such attempts produced no positive benefit, further research found in the mid twentieth century that human cells could be used to help prevent the human body rejecting transplanted organs, leading in time to successful bone marrow transplantation. (wikipedia.org)
  • Bone marrow transplants have been found to be effective, along with some other kinds of human cell therapy - for example in treating damaged knee cartilage. (wikipedia.org)
  • embryos
  • The stem cells are usually extracted from the amniotic sac by amniocentesis which occurs without harming the embryos. (wikipedia.org)
  • The use of amniotic fluid stem cells is therefore generally considered to lack the ethical problems associated with the use of cells from embryos. (wikipedia.org)
  • In developing embryos, blood formation occurs in aggregates of blood cells in the yolk sac, called blood islands. (wikipedia.org)
  • In 1931 Paul Niehans (1882-1971) - who has been called the inventor of cell therapy - attempted to cure a patient by injecting material from calf embryos. (wikipedia.org)
  • However, since they must be derived from early human embryos their production and use in research has been a hotly debated topic as the emt introduce new cells into adult bodies for possible treatment of cancer, diabetes, neurological disorders and other medical conditions. (wikipedia.org)
  • regeneration
  • Regeneration of skeletal muscle relies on a population of quiescent stem cells ( satellite cells ) and is impaired in very old (geriatric) individuals undergoing sarcopenia . (fightaging.org)
  • Global gene expression analyses uncovered an induction of p16INK4a in satellite cells of physiologically aged geriatric and progeric mice that inhibits satellite cell-dependent muscle regeneration. (fightaging.org)
  • p16INK4a silencing rejuvenated satellite cells, restoring regeneration in geriatric and progeric muscles. (fightaging.org)
  • Cellular kinetics and modeling of bronchioalveolar stem cell response during lung regeneration. (nih.gov)
  • Organ regeneration in mammals is hypothesized to require a functional pool of stem or progenitor cells, but the role of these cells in lung regeneration is unknown. (nih.gov)
  • For human tissue regeneration to become a viable therapy for conditions like neurodegeneration or diabetes, clinicians will likely need to artificially manipulate factors that return adult brain or pancreatic cells to a cellular "Tir Nan Og" to restore adult cell types. (salk.edu)
  • Suzuki, possibly inspired by his new job as a research scientist at the Research Unit for Organ Regeneration in Kobe, Japan, explains it this way: "Just as humans can start over in life, differentiated cells can also take on other fates following the generation of undifferentiated stem cells. (salk.edu)
  • Decline in the proportion of healthy cardiac stem cells (CSCs) can affect tissue regeneration. (fightaging.org)
  • In the study, researchers tagged cells using a transposon, a piece of genetic code that can jump to a random point in DNA when exposed to an enzyme called transposase, to track blood progenitors and adult stem cells during the natural, unperturbed process of blood regeneration. (eurekalert.org)
  • The cells also have potential medical applications, especially in organ regeneration. (wikipedia.org)
  • pluripotency
  • Nanog is a critical factor required for what cell biologists call "stemness," which is defined by two qualities: the ability of ES cells to divide or "self-renew" and their plasticity in assuming the identity of almost any cell type, which is also known as "pluripotency. (salk.edu)
  • But when those maturing cells were forced to produce Nanog, they reverted to their naïve state and regained pluripotency. (salk.edu)
  • He is able to de-bookmark using small-molecule chemicals that mildly target the binding pockets of the bookmarking proteins, known as bromodomains extra terminal, or BET. The result, Hu says in a paper published in Cell Reports on Sept. 20, is a proof-of-principle strategy to facilitate reprogramming to pluripotency. (uab.edu)
  • vitro
  • Although chemical labeling methods such as ICAT (isotope-coded affinity tags) have demonstrated widespread applicability ( 1 ), metabolic incorporation strategies such as stable isotope labeling with amino acids in cell culture (SILAC) are becoming more common for cell types that can be grown for extensive periods of time in vitro . (mcponline.org)
  • Mesenchymal stem cells from Bichat's fat pad: in vitro comparison with adipose-derived stem cells from subcutaneous tissue," BioResearch Open Access , vol. 2, no. 2, pp. 107-117, 2013. (hindawi.com)
  • tissues
  • Stem cell function is essential for organismal homeostasis , providing a renewable source of cells to repair damaged tissues. (fightaging.org)
  • senescent cells accumulate in tissues, and their inflammatory and other disruptive signaling causes considerable harm. (fightaging.org)
  • A fibroblast is a type of cell that synthesizes the extracellular matrix and collagen, the structural framework (stroma) for animal tissues, and plays a critical role in wound healing. (wikipedia.org)
  • gene expression
  • The team, headed by professor Juan Carlos Ispizúa Belmonte , Ph.D., of the Gene Expression Laboratory and including professor Fred Gage , Ph.D., of the Laboratory of Genetics, demonstrates how a DNA-binding protein called Nanog coaxes mouse ES cells trying to differentiate into muscle cells back into an immature state. (salk.edu)
  • The team found that in ES cells attempting to differentiate prematurely, Nanog actually binds to Smad1 protein and interferes with its ability to recruit those obligatory coactivators, thereby rendering Smad1 powerless to initiate muscle gene expression. (salk.edu)
  • Cells use bookmarks too, specific proteins that help the cell remember what collection of genes needs to be turned on again after the brief halt of gene expression during cell division. (uab.edu)
  • Therapy
  • The findings of this research, if applicable to humans, will have implications for blood cell transplantation, and for clinical and research methods using blood cells, such as gene therapy or gene editing," said John W. Thomas, Ph.D., Stem Cell and Cell-based Therapy Coordinator at NHLBI. (eurekalert.org)
  • Moving forward, we need to come up with methods to better predict what types of cells will be the most optimal for therapy, for instance in reprogramming cells, and editing," he said. (eurekalert.org)
  • He is the founder and CEO of AgeX Therapeutics a startup focused on the field of interventional gerontology, and Co-CEO of its parent company, BioTime, Inc. (AMEX: BTX) of Alameda, California (San Francisco Bay Area), a biotechnology company regarded as a leader in the field of regenerative medicine with a focus on cell therapy. (wikipedia.org)
  • Microtransplantation(MST) is an advanced technology to treat malignant hematological diseases and tumors by infusing patients with granulocyte colony-stimulating factor (G-CSF) mobilized human leukocyte antigen (HLA)-mismatched allogeneic peripheral blood stem cells following a reduced-intensity chemotherapy or targeted therapy. (wikipedia.org)
  • A new 37 acre Innovation complex is going to be set up as an extension to CRF, which aims to conduct translational research for stem cell therapy and Agri biotech. (wikipedia.org)
  • Cell therapy originated in the nineteenth century when scientists experimented by injecting animal material in an attempt to prevent and treat illness. (wikipedia.org)
  • Today two distinct categories of cell therapy are recognized. (wikipedia.org)
  • The first category is cell therapy in mainstream medicine. (wikipedia.org)
  • Cell therapy can be defined as therapy in which cellular material is injected into a patient. (wikipedia.org)
  • The origins of cell therapy can perhaps be traced to the nineteenth century, when Charles-Édouard Brown-Séquard (1817-1894) injected animal testicle extracts in an attempt to stop the effects of aging. (wikipedia.org)
  • In recent times, cell therapy using human material has been recognized as an important field in the treatment of human disease. (wikipedia.org)
  • The experimental field of Stem cell therapy has shown promise for new types of treatment. (wikipedia.org)
  • In mainstream medicine, cell therapy is supported by a distinct healthcare industry which sees strong prospects for future growth. (wikipedia.org)
  • In allogeneic cell therapy the donor is a different person to the recipient of the cells. (wikipedia.org)
  • Cell therapy is targeted at many clinical indications in multiple organs and by several modes of cell delivery. (wikipedia.org)
  • amniotic fluid
  • Amniotic stem cells are the mixture of stem cells that can be obtained from the amniotic fluid as well as the amniotic membrane. (wikipedia.org)
  • In 2009, the first US amniotic stem cell bank was opened in Medford, MA, by Biocell Center, an international company specializing in the cryopreservation and private banking of amniotic fluid stem cells. (wikipedia.org)
  • Haematopoietic progenitor cells were first reported to be present in the amniotic fluid in 1993, specifically up to the 12th week of pregnancy. (wikipedia.org)
  • In 1996, a study indicated that non-haematopoietic progenitor cells were also present in the amniotic fluid. (wikipedia.org)
  • The majority of stem cells present in the amniotic fluid share many characteristics, which suggests they may have a common origin. (wikipedia.org)
  • In 2007, it was confirmed that the amniotic fluid contains a heterogeneous mixture of multipotent cells after it was demonstrated that they were able to differentiate into cells from all three germ layers but they could not form teratomas following implantation into immunodeficient mice. (wikipedia.org)
  • Mesenchymal stem cells (MSCs) are highly abundant in the amniotic fluid and several techniques have been described for their isolation. (wikipedia.org)
  • They usually involve the removal of amniotic fluid by amniocentesis and their distinction from other cells may be based on their morphology or other characteristics. (wikipedia.org)
  • Originally it was proposed that the MSCs were discarded from the embryo at the end of their life cycle but since the cells remained viable in the amniotic fluid and were able to proliferate in culture this hypothesis was overturned. (wikipedia.org)
  • donor
  • The term "microtransplantation" comes from its mechanism of reaching donor cell microchimerism. (wikipedia.org)
  • Chemotherapy is used by lower doses only to destroy cancer and partially suppress patient's immune system, which will be reinitiated by donor's stem cells soon after transplantation, and will play a role as recipient-versus-tumor (RVT) effect combining donor cells' graft-versus-tumor (GVT) effect. (wikipedia.org)
  • Possible mechanisms of the successful avoidance of GVHD include donor cell microchimerism, less-toxic cells processed prior to transplantation, and the preservation of host immune system that is capable of resisting the GVH alloresponse. (wikipedia.org)
  • Moreover, as HLA-mismatched stem cells are employed, donor availability is extremely extended. (wikipedia.org)
  • The 6-year LFS and OS were 76.4% and 82.1%, respectively, in patients who received a high dose of donor CD3+ T cells, which were significantly higher than the LFS and OS in patients who received a lower dose of donor CD3+ T cells (49.5% and 55.3%, respectively). (wikipedia.org)
  • senescent cells
  • Steve - It is probably easier just to remove the senescent cells. (fightaging.org)
  • I still suspect it will be easier to remove senescent cells/damaged mitochondria/cross links/inter&intra cellular aggregates than trying to modify potentially hundreds of downstream signaling protein and molecule changes. (fightaging.org)
  • We know that these cells exert deleterious effects just like other senescent cells: both Kirkland's earlier study in the BubR1-hypotrophic mice and the newer study in mice with irradiated hindlegs subsequently administered "senolytics" found that clearing these cells improved muscle quality and exercise performance on the treadmill test. (fightaging.org)
  • Since senescent cells are a cause of chronic inflammation , and since inflammation and oxidative stress go hand in hand, near any condition in which inflammation or oxidative stress feature prominently is a good candidate for reexamination. (fightaging.org)
  • Recently, evidence has emerged for senescent cells to be involved in the growth and weakening of heart muscle that follows the age-related increases in blood pressure known as hypertension . (fightaging.org)
  • The paper here looks at oxidative stress and senescent cells on heart stem cells that occurs in rats engineered to develop hypertension. (fightaging.org)
  • The proportion of senescent cells was significantly higher in SHR compared to age matched Wistar rat. (fightaging.org)
  • Cortical
  • Through a process called 'cortical patterning', the protomap is patterned by a system of signaling centers in the embryo, which provide positional information and cell fate instructions. (wikipedia.org)
  • Overexpression
  • 3. Results of mRNA RT-PCR in MSCs showed an overexpression of IDO, HLA-G and LIF in cultures with cell contact and without cell contact. (irsn.fr)
  • In cultures with cell contact an overexpression of IL10 and TGF-β was observed, but not in cultures without cell contact. (irsn.fr)
  • viability
  • W. Strober, "Trypan blue exclusion test of cell viability," Current Protocols in Immunology , pp. (hindawi.com)
  • allogeneic
  • Indications for microtransplantation are as follows: Hematologic Malignancies Tumors Acute myeloid leukemia (AML) chronic-phase chronic myeloid leukemia (CP-CML) Myelodysplastic syndromes (MDS) Intermediate- or high-risk Non-Hodgkin lymphoma High-risk Hodgkin lymphoma Multiplemyeloma (MM) Severe aplastic anemia (SAA) The cells employed are allogeneic peripheral blood stem cells. (wikipedia.org)
  • regenerate
  • The scientists discovered that these cells regenerate differently than their counterparts do after a blood cell transplant, according to a study published Jan. 3 in Nature and funded by the National Heart, Lung and Blood Institute (NHLBI), part of the National Institutes of Health. (eurekalert.org)
  • research
  • Quality reprogramming is a vital step in efforts to use iPS cells in medical research and disease treatments. (uab.edu)
  • Characterization of dental pulp stem cells of human tooth germs," Journal of Dental Research , vol. 87, no. 7, pp. 676-681, 2008. (hindawi.com)
  • Future research may reveal important interactions between migrating cells and self-assembled nanostructures. (wikipedia.org)
  • Prior to joining BioTime, West was Chairman of the Board, Chief Scientific Officer and CEO of Advanced Cell Technology (ACT), another biotechnology company focused on stem cell research. (wikipedia.org)
  • A decade of stem-cell research. (wikipedia.org)
  • Established in 2001, The Stem Cell Network (SCN) has become Canada's foremost research organization dedicated to enabling the translation of stem cell research into clinical applications, commercial products and public policy. (wikipedia.org)
  • Supported by the Government of Canada, SCN has facilitated the growth of Canada's stem cell research community, and has provided approximately $85 million for innovative, translational research. (wikipedia.org)
  • Additionally, SCN has catalyzed 12 clinical trials, 11 start-up companies, incubated several international and Canadian research networks and organizations, and established the Till & McCulloch Meetings, Canada's foremost stem cell research event. (wikipedia.org)
  • The mission of the organization is "to be a catalyst for enabling translation of stem cell research into clinical applications, commercial products or public policy. (wikipedia.org)
  • The organizations shared information about their mandates and the status of stem cell research in their respective countries, and concluded that (i) they had a responsibility to do all that they could to foster international research collaborations between networks and investigators, and (ii) there was a significant opportunity to share best practice and collaborate in other areas to achieve individual missions more rapidly and effectively. (wikipedia.org)
  • These stem cells can differentiate into all other cells in the human body and are the subject of much scientific research. (wikipedia.org)
  • No federal law ever did ban stem cell research in the United States, but only placed restrictions on funding and use, under Congress's power to spend. (wikipedia.org)
  • On July 18, 2006, the Senate passed three different bills concerning stem cell research. (wikipedia.org)
  • lymphocyte
  • The potential of the method was demonstrated by investigating cytogenetic and physiological changes of human lymphocyte cells as well as conducting comprehensive genetic, genomic and physiological state studies of human cells and cells of the model yeast Saccharomyces cerevisiae. (wikipedia.org)
  • oxidative
  • As aged cells have less regenerative capacity and are more susceptible to oxidative stress, we investigated the effect of ageing on SIRT3 levels and its correlation with antioxidant enzyme activities. (sigmaaldrich.com)
  • SIRT3 expression, levels of MnSOD and CAT, as well as cell survival showed little difference in old versus young hMSCs under normal growth conditions, whereas older cells had a significantly reduced capacity to withstand oxidative stress compared to their younger counterparts. (sigmaaldrich.com)
  • Both Jun and its dimerization partners in AP-1 formation are subject to regulation by diverse extracellular stimuli, which include peptide growth factors, pro-inflammatory cytokines, oxidative and other forms of cellular stress, and UV irradiation. (wikipedia.org)
  • immune
  • Landscape of the lineage fate (natural environment) of unperturbed haematopoiesis (the process of mature blood and immune cell production). (eurekalert.org)
  • In this report, we review the literature identifying stimulatory effects of mesenchymal stem cells (MSC) on immune responses and explore the potential for MSC as a novel, universal vaccination platform. (frontiersin.org)
  • G-CSF is produced by endothelium, macrophages, and a number of other immune cells. (wikipedia.org)
  • Immune cells then respond and initiate a cascade of events to clear the invasive microorganisms. (wikipedia.org)
  • regulates
  • This suggests that c-jun regulates cell cycle progression and apoptosis through two separated mechanisms. (wikipedia.org)