Cellular proteins polypyrimidine tract binding protein. Medical search. Frequent questions

Initiation of BAG-1 protein synthesis can occur by both cap-dependent and cap-independent mechanisms and it has been shown that synthesis of BAG-1S is dependent upon the presence of an internal ribosome entry segment (IRES) in the 5′-UTR of BAG-1 mRNA. (nature.com)
2002). Translation of polioviral mRNA is inhibited by cleavage of polypyrimidine tract-binding proteins executed by polioviral 3C(pro). (nature.com)
Results of in vivo transfection of these bicistronic constructs suggested the presence of two IRES elements within p53 mRNA, with activities comparable to known viral and cellular IRESs. (iisc.ac.in)
The IRES directing the translation of p53 is in the 5'-untranslated region of the mRNA, whereas the IRES mediating the translation of ΔN-p53 extends further into the protein-coding region. (iisc.ac.in)
Proteins shown in red (FDR 0.01) represent the mRNA interactome. (nature.com)
c ) Overlap of mRNA interactome proteins in yeast and HuH-7. (nature.com)
d ) Validation of the yeast mRNA interactome using western blotting of input samples and eluate after interactome capture with specific antibodies (ADH1, alcohol dehydrogenase 1, PUB1) or against TAP-tagged proteins (PGK1, phosphoglycerate kinase 1, TDH1, triose phosphate dehydrogenase, TRX2, thioredoxine 2, SHE2, Swi5p-dependent HO Expression 2). (nature.com)
The regulation of expression of X-box-binding protein-1 (XBP1), a transcriptional factor, involves an unconventional mRNA splicing that removes the 26 nucleotides intron. (mdpi.com)
Phosphorylation by mTOR of 4E-BP1 disrupts its binding to eIF4E, a protein that binds the 5'-cap structure of mRNA. (biomedcentral.com)
It binds the 3′ AG splice acceptor dinucleotide of the pre-mRNA target intron, and forms a heterodimer with U2AF2 , which binds the adjacent polypyrimidine tract. (massgenomics.org)
It is transcribed in a 14 kb mRNA, and the 11kb cDNA encodes a 3685 amino acid protein of 427 kDa called Dystrophin. (pressbooks.pub)
This machinery recognizes pre-mRNA sequences at several motifs - the 5′ and 3′ splice sites, the branch point, and polypyrimidine tracts - positioned at exon-intron boundaries [ 4 ]. (biomedcentral.com)
These proteins bind specific sequences in the pre-mRNA called intronic or exonic splicing enhancer or LY404039 suppressor sequences. (biomedigs.org)
Splice site selection will reflect the relative occupation of these sequences and LY404039 interactions among different proteins on a pre-mRNA (Witten and Ule, 2011). (biomedigs.org)
MicroRNAs regulate gene expression at the post-transcriptional level by binding to 3′- or less often to 5′-untranslated regions of target messenger RNAs (mRNAs), which in consequence leads to inhibited translation and/or induces degradation of targeted mRNA [ 1 ]. (archivesofmedicalscience.com)
A microRNA strand is transferred to the Argonaute complex (AGO), forming an RNA-induced silencing complex (RISC), and guides it to pair with the target mRNA through binding of the microRNA seed sequence with the microRNA recognition site on the mRNA. (archivesofmedicalscience.com)

U2AF35, the small subunit of U2AF, binds to the intron/exon border, whereas the large subunit U2AF65 binds to a region rich in pyrimidines designated the polypyrimidine tract (Figure 1). (biomedigs.org)
It specifically binds Na+/K+-ATPase -subunit to inhibit Na+/K+-ATPase, which has been explored in a number of clinical studies for cancers treatment today. (tech-strategy.org)

The large ~280-kDa U5 snRNP protein PRPF8 is central to the dynamics of spliceosome assembly [ 11 ]. (biomedcentral.com)
The core particles of the U1, U2, U4, and U5 snRNPs are formed by Sm proteins, whereas the LY404039 U6 snRNP contains the related Lsm2 (Like Sm2) to Lsm8 proteins (Tharun, 2009). (biomedigs.org)
The initial step of splice site recognition comprises U1 snRNP binding to the 5splice site and U2 auxillary factor (U2AF) binding to the 3splice site. (biomedigs.org)
Subsequently, U2 snRNP binds to the branch point, and a preformed complex of U4, U5, and U6 snRNPs is recruited to the intron. (biomedigs.org)
The spliceosomes consist of four small nuclear ribonucleoproteins (snRNPs) and over a hundred of non-snRNP proteins that associate with snRNPs at some point during the splicing [ 4 - 6 ]. (ijbs.com)

2006). Polypyrimidine tract binding protein regulates IRES-mediated gene expression during apoptosis. (nature.com)
These results suggest that GAPDH exerts other functions beyond glycolysis, and that oxidatively modified GAPDH regulates its cellular functions by changing its interacting proteins, i.e. the RNA splicing by interacting with the p54nrb-PSF complex. (elsevierpure.com)

Alternative polyadenylation alters protein dosage by switching between intronic and 3'UTR sites. (rochester.edu)

In order to obtain more detailed information regarding the interaction between host proteins and the defined viral nucleic acid and/or proteins, we investigated the host factors associating with the main replicase enzyme, NS5, in the JEV-infected cells using yeast-two hybrid screening. (biomedcentral.com)
The decision on which splice sites are selected under particular cellular conditions is determined by the interaction of additional proteins, globally designated as splicing factors (SFs), that guide spliceosomal components and thereby the spliceosome to the respective splice sites (Matlin et al. (biomedigs.org)

Polypyrimidine tract binding protein and associated splicing factor (PSF) has diverse functions inside the cell such as transcriptional regulation, DNA repair, acts as nucleic acids binding protein and regulate replication and infectivity of different viruses. (biomedcentral.com)
Insect genes that harbor U12-type introns belong to several functional categories among which proteins binding ions and nucleic acids are enriched and these few categories are also overrepresented among these genes that preserved minor type introns in Diptera . (ijbs.com)

This process is carried out by the human spliceosome machinery, in which over 300 proteins sequentially assemble with uridine-rich small nuclear RNA molecules (U snRNAs) to form distinct small nuclear ribonucleoprotein complexes (snRNPs). (biomedcentral.com)
It consists of five small nuclear ribonucleoprotein particles (snRNPs) and over 200 additional proteins (Wahl et al. (biomedigs.org)
2012). The main families of these SFs are the Ser/Arg-rich (SR) proteins and heterogeneous nuclear ribonucleoprotein particle (hnRNP) proteins. (biomedigs.org)

The different interactions between viral proteins and cellular host proteins are required for efficient replication of HIV-1. (biomedcentral.com)
The integration process is catalyzed by viral protein integrase which utilizes different host proteins for integration of viral DNA [ 1 ]. (biomedcentral.com)

Only 60% of yeast and 73% of the human RBPs have functions assigned to RNA biology or structural motifs known to convey RNA binding, and many intensively studied proteins surprisingly emerge as RBPs (termed 'enigmRBPs'), including almost all glycolytic enzymes, pointing to emerging connections between gene regulation and metabolism. (nature.com)

In particular, our analysis points to polypyrimidine tract binding protein 1 as a regulator of poly(A) site choice in glioblastoma. (nih.gov)
Further, it has been shown that polypyrimidine tract binding (PTB) protein acts as an important regulator of p53 IRES activities. (iisc.ac.in)
Some studies also suggest that mTOR may be a cellular context-dependent, pleiotropic regulator of apoptosis, although conclusive demonstration of mTOR inactivation in such circumstances is lacking [ 19 ]. (biomedcentral.com)

Here, we show that alterations in RNA splicing patterns across the human transcriptome that occur in conditions of restricted cellular PRPF8 abundance are defined by the altered splicing of introns with weak 5′ splice sites. (biomedcentral.com)

GAPDH (glyceraldehyde-3-phosphate dehydrogenase) is known as a major target protein in oxidative stresses and becomes thiolated in its active site. (elsevierpure.com)
We identified the interacting proteins of oxidized inactive GAPDH as p54nrb (54 kDa nuclear RNA-binding protein) and PSF (polypyrimidine tract-binding protein-associated splicing factor), both of which are known to exist as heterodimers and bind to RNA and DNA. (elsevierpure.com)
The C-terminal of p54nrb binds to GAPDH in the cytosol in amanner dependent on the dose of hydrogen peroxide. (elsevierpure.com)
The GAPDH-p54nrb complex enhances the intrinsic topoisomerase I activation by p54nrb-PSF binding. (elsevierpure.com)
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a cellular metabolic protein, was found to colocalize with viral RNA-dependent RNA polymerase (NS5) in JEV-infected cells. (biomedcentral.com)
instead, GAPDH binds to the 3' termini of plus- and minus-strand RNAs of JEV by electrophoretic mobility shift assays. (biomedcentral.com)
Accordingly, GAPDH binds to the minus strand more efficiently than to the plus strand of JEV RNAs. (biomedcentral.com)

RNA-binding proteins (RBPs) mediate pivotal cellular functions such as RNA transport, degradation or translation and represent key effectors of post-transcriptional gene regulation. (nature.com)
CCCTC-binding factor (CTCF) is a conserved transcription factor that performs diverse roles in transcriptional regulation and chromatin architecture. (preprints.org)
Characterization of DNA binding, transcriptional activation, and regulated nuclear association of recombinant human NFATp. (colorado.edu)

Numerous studies have shown that replication of RNA viruses is involved in many specific RNA-RNA, RNA-protein, and protein-protein interactions. (biomedcentral.com)
No yeast two-hybrid interactions found for this protein. (yeastrc.org)

3' Untranslated regions (3' UTRs) length is regulated in relation to cellular state. (nih.gov)

RNA-binding proteins (RBPs) exert a broad range of biological functions. (nature.com)
Recently developed unbiased high content techniques to identify RBPs in vivo yielded information on differences in cell type-specific expression and/or RNA-binding activity of RBPs in mammalian cells 2 , 3 . (nature.com)
For example, in both animals and plants, many SFs/RNA binding proteins (RBPs) and some core spliceosomal components themselves undergo AS in response to signals and even control their own levels and those of other SFs via AS (Kalyna et al. (biomedigs.org)

We show that this conserved RNA interactome harbours many proteins without previously assigned roles in RNA biology (enigmRBPs), including surprisingly many metabolic enzymes. (nature.com)
Until the discovery of ribozymes, it had been assumed that enzymes could only be generated with the diversity of structures possible with the amino acids in proteins. (pressbooks.pub)
It is estimated that microRNAs regulate production of approximately 60% of all human proteins and enzymes that are responsible for major physiological processes. (archivesofmedicalscience.com)

We have shown previously that BAG-1 IRES-meditated initiation of translation requires two trans -acting factors poly (rC) binding protein 1 (PCBP1) and polypyrimidine tract binding protein (PTB) for function. (nature.com)
viral proteins may decoy some host factors for assisting viral replication. (biomedcentral.com)

RNA level and protein contents in TAC, Dex, or combined TAC- plus Dex-treated patients and Huh-7 cells were measured utilizing quantitative real-time (qRT)-PCR and western blotting assays measured the formation of lipid droplet. (bvsalud.org)

The probable mechanism of action of PTB protein has also been investigated. (iisc.ac.in)
Identification of these interacting host cellular protein provide better understanding of mechanism of viral replication and subsequently development of new therapeutic approaches. (biomedcentral.com)
Alternative splicing is a crucial mechanism for generating protein diversity. (biologists.com)
The mechanism of attenuation of angiogenic proteins by ibuprofen was further investigated. (akjournals.com)

1999). Cellular stress in Xenopus kidney cells enhances the phoshorylation of eukaryotic initiation factor eIF(4E) and the association of eIF4F with poly(A)-binding protein. (nature.com)
Most eukaryotic protein coding genes are intervened by non-coding sequences called introns ( Int ervening r egi ons ) [ 1 ], which are being removed from the primary transcript in the process of splicing [ 2 - 3 ]. (ijbs.com)

For example, a word in an open reading frame (ORF) has a direct influence on the primary amino acid sequence of a protein and hence is under strong selective pressure. (biomedcentral.com)

Here, we show that the BAG-1 IRES maintains synthesis of BAG-1 protein following exposure of cells to the chemotoxic drug vincristine but not to cisplatin and that this is brought about, in part, by the relocalization of PTB and PCBP1 from the nucleus to the cytoplasm. (nature.com)
One of the important mechanisms used for protein synthesis during these conditions is internal initiation. (iisc.ac.in)
Therefore, appropriate control of their synthesis is required to assure the complex orchestration of cellular processes within multicellular organisms. (biologists.com)
In many cell lines, exposure to rapamycin results in a relatively small decrease in overall protein synthesis (~15-20%), but dose result specifically in G1 cell cycle arrest. (biomedcentral.com)

By means of oligonucleotide microarray and RNA interference, we reveal that the sensitizing effect of IFN-β was possibly due to attenuation of MGMT expression via induction of the protein p53. (aacrjournals.org)

Clearly, differences in the abundance, localization, and activity of proteins in different cells or in cells experiencing different internal or external cues will affect the splicing outcomes. (biomedigs.org)

A beta-hairpin comprising the nuclear localization sequence sustains the self-associated states of nucleosome assembly protein 1. (colorado.edu)

There are three major isoforms of BAG-1 in mammalian cells, termed BAG-1L (p50), BAG-1M (p46) and BAG-1S (p36) that function as pro-survival proteins and are associated with tumorigenesis and chemoresistance. (nature.com)
The PI3K/Akt/mTOR signal pathway is involved in multiple cellular functions including proliferation, differentiation, tumorigenesis, and apoptosis. (biomedcentral.com)

Splicing factor, proline- and glutamine-rich is a protein that in humans is encoded by the SFPQ gene. (wikipedia.org)

Tumor suppressor p53, the guardian of the genome, has been intensely studied molecule owing to its central role in maintaining cellular integrity. (iisc.ac.in)

The transcriptomic and epigenomic regulatory landscape of the MAC genome, which has 80% protein-coding genes and short intergenic regions, is poorly understood. (uni-frankfurt.de)
Mutations in cis-acting splicing elements or changes in the activity of constitutive or alternative splicing could have a profound regulatory proteins that compromise the accuracy of either impact on human pathogenesis, in particular in tumor development and progression. (biologists.com)

To address the second, we investigated RNAs bound by the metabolic enzyme hydroxysteroid dehydrogenase 17-β 10 (HSD17B10). (nature.com)

Analyses of the mitochondrial hydroxysteroid dehydrogenase (HSD17B10) uncover the RNA-binding specificity of an enigmRBP. (nature.com)
We also determined the RNA targets of an RNA-binding mitochondrial enzyme and show its specificity in RNA binding. (nature.com)

The cellular proliferation marker Ki-67 was decreased robustly in ATP1B2-suppressed tumors (Number 6G). (tech-strategy.org)

In the present study, we have identified a new HIV-1 integrase interacting protein, polypyrimidine tract binding protein and associated splicing factor (PSF) through pull down assay and co immunoprecipitation of IN with mammalian cell protein. (biomedcentral.com)

Additionally, U12-type introns lack a polypyrimidine tract between the BPS and the 3′ splice site. (ijbs.com)

After cell lysis, polyadenylated RNAs were captured on oligo d(T) beads followed by stringent washes to remove non-crosslinked proteins. (nature.com)

In an important model of premature aging, Werner's Syndrome, the genetic problem seems to be lack of a helicase protein that can unwind G-quadruplexes. (anti-agingfirewalls.com)

In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. (thermofisher.com)

The former protein allows BAG-1 IRES RNA to attain a structure that permits binding of the ribosome, while the latter protein appears to be involved in ribosome recruitment. (nature.com)

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. (thermofisher.com)

In an attempt to study the host interacting protein of IN, we have identified a new interacting host protein PSF which is a splicing factor and elucidated its role in integration and viral replication. (biomedcentral.com)

While the level of p53 protein is maintained low in unstressed conditions, there is a rapid increase in the functional p53 protein levels during stress conditions. (iisc.ac.in)
The structural and functional integrity of this overhang is based on its association with a constellation of specific proteins, some of them belonging to the shelterin complex [ 10 ]. (hindawi.com)

Stimulated by the pioneering studies on the cationic porphyrin TMPyP4 and the natural product telomestatin, follow-up studies have developed, rapidly leading to a large diversity of macrocyclic structures with remarkable-quadruplex binding properties and biological activities. (hindawi.com)

Different splice variants of a given protein can display different and even antagonistic biological functions. (biologists.com)
In this review we summarize the current state of the art in detailing the three main categories of quadruplex-binding macrocycles described so far (telomestatin-like polyheteroarenes, porphyrins and derivatives, polyammonium cyclophanes), and in addressing both synthetic issues and biological aspects. (hindawi.com)

It is now well documented in literature that p53 protein accumulates in the cells following DNA damage by posttranslational modifications leading to increased stability and half life of protein. (iisc.ac.in)

Treating SMMC-7721 cells with Rapamycin, a specific mTOR inhibitor, significantly reduce hTERT protein level but did not affect hTERT transcription. (biomedcentral.com)

Anatomy5

Organisms1

Chemicals and Drugs15

Phenomena and Processes14

Information Science3

MRNA16

Subunit2

SnRNP5

Regulates2

Polyadenylation1

Interaction2

Nucleic acids2

Nuclear ribonucleoprotein3

Host proteins2

Motifs1

Regulator3

Splice sites1

GAPDH7

Transcriptional3

Interactions2

Untranslated Regions1

RBPs3

Enzymes3

Factors2

Assays1

Mechanism4

Eukaryotic2

Amino1

Synthesis4

Attenuation1

Abundance1

Nucleosome1

Tumorigenesis2

Humans1

Role in maintain1

Regulatory2

Metabolic1

Specificity2

Proliferation1

Mammalian1

Additionally1

RNAs1

Helicase1

Associate1

IRES1

Knockdown1

Viral replication1

Functional2

Diversity1

Biological2

Stability1

Specific1