• A nanoscale modular design strategy was employed to synthesize six engineered, recombinant proteins intended to mimic aspects of the extracellular matrix proteins fibronectin, laminin, and elastin as well as the cell-cell adhesive protein neural cell adhesion molecule. (frontiersin.org)
  • It constitutes fibrous proteins such as collagen and elastin, elongated glycoproteins such as fibronectin and laminin, which provide cell matrix adhesion. (biomedcentral.com)
  • The aim of the present study was to functionalize Ti scaffolds with genetically modified elastin-like recombinamers (ELRs), synthetic polymeric proteins containing the elastin epitopes responsible for their mechanical properties and for promoting mesenchymal stem cell (MSC) recruitment, proliferation, and differentiation to ultimately increase scaffold osseointegration. (ibecbarcelona.eu)
  • This growth factor also stimulates production of collagens, proteoglycans, elastin, fibronectin, tenascin and thrombospondin, diminishes production of extracellularly active neutral endoproteinases belonging to the matrix metalloproteinase (MMP) and serine proteinase families, and stimulates production of endogenous MMP inhibitors (tissue inhibitor of metallo-proteinase [TIMP]) and serpins (plasminogen activator inhibitor-1). (biomedcentral.com)
  • Consistent with this hypothesis, developing granule cells from the rostral region of Gpr56 −/− cerebella at birth exhibit a specific defect in cell adhesion to extracellular matrix (ECM) molecules of the pial basement membrane (BM) but no defects in migration, proliferation, or process outgrowth. (jneurosci.org)
  • SPARC (secreted protein,acidic and rich in cysteine) is a matricellular glycoprotein that regulates cell-cell and cell-matrix interactions, cellular proliferation and differentiation, and the expression of genes encoding extracellular matrix components. (silverchair.com)
  • Deposition of collagen and proliferation of smooth muscle cells are the response to chronic inflammation and injury of the submucosa leading to fibrosis and thickening of the submucosa and the smooth muscle layers of the bowel wall. (bmj.com)
  • The TGF-β family forms an important group of growth factors, consisting of three isoforms in man, and is important for matrix deposition because it modulates fibroblast recruitment and proliferation. (biomedcentral.com)
  • Lastly, we revealed that addition of type IV collagen protein induced myofibroblast proliferation and migration in monolayer culture and increased the formation of mesenchymal-epithelial septal-like structures in co-culture. (biomedcentral.com)
  • In addition to its role in the formation of epithelium and vasculature, type IV collagen appears to be key for alveolar myofibroblast development by inducing their proliferation, differentiation and migration throughout the developing septum. (biomedcentral.com)
  • It may also play a role in cell interactions with the extracellular matrix. (wikipedia.org)
  • The enzymatic isolation process disrupts islet cell membranes, damages cell-to-cell interactions and inhibits vital functions. (brighton.ac.uk)
  • Therefore, this engineered protein adsorption approach allows for the facile preparation of tunable, quantifiable, and reproducible surfaces for in vitro studies of cell-ligand interactions and for potential application as coatings on neural implants. (frontiersin.org)
  • It is influenced by angiogenesis, cell-cell and cell-extra cellular matrix (ECM) interactions. (biomedcentral.com)
  • On the other hand, even if 3D aggregate culture methods are able to recapitulate in vitro the cell-extracellular matrix interactions properly observed in vivo, and the synthetic/natural matrix and scaffolds have biochemical and mechanical properties, in order to mimic the native extracellular matrix, both of these systems possesses some limitations and some methodological improvements are needed. (razavihospital.ir)
  • Nidogen-1 (NID-1), formerly known as entactin, is a protein that in humans is encoded by the NID1 gene. (wikipedia.org)
  • The resistance to fouling is quantified and thrombus formation and deposition of blood cellular components on the coatings are analyzed. (ibecbarcelona.eu)
  • Indeed, granule cells from the rostral region of perinatal Gpr56 −/− cerebella show loss of adhesion to extracellular matrix molecules of the pial basement membrane. (jneurosci.org)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • This study aims to investigate the effects of oral administration of sodium nitrite on expression of laminin α5 in extracellular matrix of mice testicular interestitium using an Immunohistochemical (IHC) assessment. (biomedres.info)
  • Their localisation in relation to extracellular matrix components was shown by immunohistochemical double labelling. (bmj.com)
  • in this manner we show that fibrotic matrices clearly promote transforming growth factor-β-independent myofibroblast differentiation compared with normal matrices. (atsjournals.org)
  • Czyz J, Wobus A. Embryonic stem cell differentiation: the role of extracellular factors. (razavihospital.ir)
  • We further found that the pericytic laminin isoforms differentially regulate mouse β-cells. (nature.com)
  • In C. elegans , the ten-1 gene can be transcribed from two distinct promoters to produce the transcripts ten-1a and ten-1b , which respectively encode two isoforms of the protein: TEN-1L, which contains all the teneurin domains described above (see Fig. 1F ), and TEN-1S, which lacks the ICD but contains the rest of the protein including the transmembrane domain. (biomedcentral.com)
  • Results: The processes by which re-aggregated adult single cell types or adult and embryonic explanted tissues are able to recapitulate embryogenesis in vitro, when cultured in adhesion or embedded in 3D gels, is not surprising and is clearly under the control of a reminiscent cellular memory which recapitulates early developmental stages. (razavihospital.ir)
  • It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. (cancer-genetics.org)
  • Using mass spectrometry, quantitative proteomic analysis and Gene Ontology terms filters, we observed that EVs released by EGFRvIII-transformed cells were enriched for extracellular exosome and focal adhesion related proteins. (cancer-genetics.org)
  • The IHC tests showed that the ECM of the mice testicular interstitial tissue showed reaction to anti-laminin-α5 antibody. (biomedres.info)
  • Kruskal Wallis test showed no significant difference in laminin α5 expression in the ECM of the interstitial spaces of seminiferous tubules between the groups. (biomedres.info)
  • High concentration of sodium nitrate did not change the laminin α5 expression in the ECM of the testicular interstitial tissue. (biomedres.info)
  • Here, we show that pericytes produce multiple components of the mouse pancreatic and islet interstitial and BM matrices. (nature.com)
  • Extracellular matrix (ECM) is a dynamic tissue that contributes to organ integrity and function, and its regulation of cell phenotype is a major aspect of cell biology. (atsjournals.org)
  • We define a new method to prepare acellular lung matrix that can be used to study the impact of extracellular matrix on lung cell phenotype. (atsjournals.org)
  • The link between oncogenic RAS expression and the acquisition of the invasive phenotype has been attributed to alterations in cellular activities that control degradation of the extracellular matrix. (oncotarget.com)
  • Cancer cells that have acquired an invasive phenotype release proteases that attack and digest the protein component of the matrix that would restrict the movement of these malignant cells. (oncotarget.com)
  • Mutations in GPR56 , an orphan G-protein-coupled receptor (GPCR), cause bilateral frontoparietal polymicrogyria (BFPP), a disorder characterized by mental retardation, seizures, motor developmental delay, and ataxia. (jneurosci.org)
  • Scope includes mutations and abnormal protein expression. (cancer-genetics.org)
  • We used histological, molecular, and behavioral approaches to characterize the defects caused by loss of GPR56 and also examined the potential cellular functions of this molecule in vitro and in vivo . (jneurosci.org)
  • By using human lungs and cells, this new system provides a novel way to culture cells in vitro to investigate matrix contributions to lung pathology in a disease-specific manner. (atsjournals.org)
  • Structurally it (along with perlecan) connects the networks formed by collagens and laminins to each other. (wikipedia.org)
  • Matrix metalloproteinases (MMPs) are a group of structurally related endopeptidases that require a metal cofactor. (medscape.com)
  • Some cytokines act as competence rather than progression factors, some lack secretory signals, and some must be processed and released from the pericellular matrix or basement membranes (eg transforming growth factor beta [TGF-β ] binding to chondroitin or the keratan sulfate of biglycan, decorin and fibromodulin, or basic fibroblast growth factor and platelet derived growth factor binding to the heparin sulfate of glypican, perlecan and syndecan). (biomedcentral.com)
  • Epithelial cells adjacent to the area of the defect flatten, lose their hemidesmosome attachments, and migrate on transient focal contact zones that are formed between cytoplasmic actin filaments and extracellular matrix proteins. (medscape.com)
  • It is now well established that a key requirement for invasion and metastasis of cancer cells is the degradation of both the extracellular matrix (ECM) that surrounds the tumor and the basement membrane which presents the final barrier between the tumor cells and the blood stream [ 1 - 5 ]. (oncotarget.com)
  • Degradation of the ECM facilitates invasion of tumor cells into the organ parenchyma, whereas degradation of the basement membrane is required for their entry into the blood stream as well as their metastasis to distant organs. (oncotarget.com)
  • Degradation of crystallins and MIP 26, or changes in the levels of these proteins, were not detected. (silverchair.com)
  • Degradation of matrix is a key event in progression, invasion and metastasis of potentially malignant and malignant lesions of the head and neck. (biomedcentral.com)
  • They are activated by proteolytic cleavage of the N-terminal region in the extracellular compartment. (medscape.com)
  • All of these alterations have deep effects on muscle proteins and also on proteolytic enzyme systems (Huff Lonergan et al. (earthwormexpress.com)
  • The protein interacts with several other components of basement membranes. (wikipedia.org)
  • IMs are found in the space between the tissue stroma cells and contain, among other components, fibrillar collagens and fibronectin. (nature.com)
  • Thus, human endocrine cells are not in direct contact with the vascular BM components but with the invaginated peri-islet membrane. (nature.com)
  • The aim of this study was to investigate whether mast cells accumulate in these tissues and whether their localisation is associated with extracellular matrix components. (bmj.com)
  • Fibrosis is characterised by an excessive production of extracellular matrix components, and cells in the tissue are constantly interacting with these extracellular matrix proteins. (bmj.com)
  • The main function of metalloproteinases is to degrade extracellular matrix and basement membrane components. (medscape.com)
  • and ( 3 ) ineffective repair of elastic fibers and perhaps other extracellular matrix components. (atsjournals.org)
  • PBP 10, an antibacterial, cell membrane-permeant rhodamine B-conjugated peptide derived from the polyphosphoinositide binding site of gelsolin, interacts selectively with both lipopolysaccharides (LPS) and lipoteichoic acid (LTA), the distinct components of gram-negative and gram-positive bacteria, respectively. (upenn.edu)
  • As confirmation that ligand density in these engineered systems impacts neuronal cell behavior, we demonstrate that increasing the density of fibronectin-derived RGD ligands on coated surfaces while maintaining uniform protein surface coverage results in enhanced neurite extension of PC-12 cells. (frontiersin.org)
  • This review critically focuses on opportunities to employ protein-graphene oxide structures either as nanocomposites or as biocomplexes and highlights the effects of carbonaceous nanostructures on protein conformation and structural stability for applications in tissue engineering and regenerative medicine. (mdpi.com)
  • Muscle cells, called muscle fibers, are long, narrow, very specialized cells covered by the cell membrane (sarcolemma), whose cytoplasm (sarcoplasm) contains the organelles and the structures responsible of muscle contraction, the myofibrils. (earthwormexpress.com)
  • This is, at least in part, attributable to the release and intercellular trafficking of extracellular vesicles (EVs), heterogeneous membrane structures containing multiple bioactive macromolecules. (cancer-genetics.org)
  • Tissue inhibitors of matrix metallo-proteinases (TIMPs) are known to have the ability to inhibit the catalytic activity of MMPs. (biomedcentral.com)
  • Turpeenniemi-Hujanen et al suggested that the expressions of matrix expression of MMPs as well as their tissue inhibitors the TIMPs are associated with the clinical behaviour in head and neck malignancy [ 12 ]. (biomedcentral.com)
  • This process results in a more physiologic substrate that retains normal stiffness, architecture, and matrix composition compared with native lungs. (atsjournals.org)
  • It is possible that ten-1 influences the composition/distribution of extracellular matrix. (biomedcentral.com)
  • We observed that EGFRvIII expression alters the expression of EV-regulating genes (vesiculome) and EV properties, including their protein composition. (cancer-genetics.org)
  • Physical adsorption isotherms were experimentally determined for these engineered proteins, allowing for direct calculation of the available ligand density present on coated surfaces. (frontiersin.org)
  • Teneurins are transmembrane proteins that assist morphogenetic processes in many organisms. (biomedcentral.com)
  • Teneurins have a single transmembrane domain, a very large and cleavable extracellular domain containing eight EGF repeats, four NHL domains and more than 20 YD repeats, as well as a cleavable intracellular domain (ICD) that can be translocated to the nucleus. (biomedcentral.com)
  • The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. (cancer-genetics.org)
  • This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. (cancer-genetics.org)
  • GSOs also mitigated the deleterious effects of GLU on the mitochondrial membrane potential and Cyt C release, thus alleviating mitochondrial dysfunction, and increased GSH levels and CAT activity in both cells and Drosophila brain tissue. (sdbonline.org)
  • Extracellular matrix is important in driving cellular phenotypic responses in fibrotic and other lung disorders. (atsjournals.org)
  • Due to their large surface area and ability to interact with proteins and peptides, graphene oxides offer valuable physiochemical and biological features for biomedical applications and have been successfully employed for optimizing scaffold architectures for a wide range of organs, from the skin to cardiac tissue. (mdpi.com)
  • In an effort to elucidate the mechanism of bacterial killing by PBP 10, we compared its effects on artificial lipid bilayers and eukaryotic cell membranes with the actions of the mellitin, magainin II, and LL37 peptides. (upenn.edu)
  • The hemolytic activities of the PBP 10 and LL37 peptides significantly increase when RBCs are osmotically swollen in hypotonic solution, indicating that these antibacterial peptides may take advantage of the more extended form of bacterial membranes in exerting their killing activities. (upenn.edu)
  • This finding suggests that asymmetrical distribution of phospholipids in the external membranes of eukaryotic cells may represent an important factor in determining the specificity of antibacterial peptides for targeting bacteria rather than eukaryotic cells. (upenn.edu)
  • We report that the decellularization process effectively removes cellular and nuclear material while retaining native dimensionality and stiffness of lung tissue. (atsjournals.org)
  • Defining the cells and proteinases responsible for destruction of lung extracellular matrix associated with cigarette smoking will be required for development of appropriate proteinase inhibitors for application in COPD. (atsjournals.org)
  • Lung development timeline and type IV collagen expression in the chicken and the mouse. (biomedcentral.com)
  • d - i Type IV collagen protein and mRNA expression in the lung. (biomedcentral.com)
  • In contrast, culturing on Laminin-421 induced the expression of β-cell genes, including Ins1 , MafA , and Glut2 , and significantly improved glucose-stimulated insulin secretion. (nature.com)
  • Instead, they act at the cellular blastoderm stage, and cleavage of the ICD may allow it to directly regulate the transcription of target genes in alternate parasegments. (biomedcentral.com)
  • Defects involve fusion of adjacent lobules, disrupted layering of neurons and glia, and fragmentation of the pial basement membrane. (jneurosci.org)
  • These ten-1 mutants are reported to have defects in the growth of several axons and in the development of the gonads and epidermis, and these defects correlated with disruptions in the extracellular matrix (ECM). (biomedcentral.com)
  • Matrix metalloproteinases (MMPs) are a family of zinc-dependent proteinases that are capable of cleaving all extra cellular matrix (ECM) substrates. (biomedcentral.com)
  • however, in the corneal stroma, excessive infiltration of monocytes/macrophages is considered to be unfavorable because they secrete matrix metalloproteinases (MMPs) and other proteins undesirable for tissue healing. (medscape.com)
  • Thus, PIs-ECM expression, localization and remodelling was analysed pre-and post-enzymatic digestion using qRT-PCR (for gene expression), western blotting (for protein expression) and immunocytochemistry (for cellular localization) techniques. (brighton.ac.uk)
  • Oncogenic RAS expression increased S100A10 gene expression which resulted in an increase in S100A10 protein levels. (oncotarget.com)
  • Most of the identified proteins are connected with the initial events of foreign body reaction towards biomaterial (coagulation cascade proteins, complement component, and inflammatory proteins). (ibecbarcelona.eu)
  • In contrast, in the submucosa, laminin was exclusively found in the basal lamina of blood vessels where many adherent mast cells were seen. (bmj.com)
  • Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)
  • The resultant matrices can be sectioned as uniform slices within which cells were cultured. (atsjournals.org)
  • β-Cells depend on the islet basement membrane (BM). (nature.com)
  • The peri-islet membrane, which encapsulates the islets, separates the endocrine cells from the exocrine pancreas and serves as a barrier from immune cell infiltration to the islets. (nature.com)
  • Depletion of S100A10 from RAS-transformed cells resulted in a loss of both cellular plasmin generation and invasiveness. (oncotarget.com)
  • The lens cells immediately beneath the capsule showed aberrant morphology, with numerous protrusions into the lens basement membrane. (silverchair.com)
  • All mast cells in the muscularis propria were colocalised with patches of laminin. (bmj.com)
  • The colocalisation with laminin indicates a mechanism of interaction between smooth muscle cells and mast cells that may be important in the role of mast cells in the process of fibrosis. (bmj.com)
  • Furthermore, GSOs protected cells against GLU-induced apoptosis by reducing the expression of the mitochondrial apoptosis-associated Bcl-2 family effector proteins and protected cells from GLU-induced oxidative damage by increasing the nuclear translocation of Nrf2 and HO-1 expression. (sdbonline.org)
  • This protein enhances cell motility, invasion and metastasis of cancer cells. (cancer-genetics.org)
  • The Impact of Oncogenic EGFRvIII on the Proteome of Extracellular Vesicles Released from Glioblastoma Cells. (cancer-genetics.org)
  • Among them, we validated the association of pro-invasive proteins (CD44, BSG, CD151) with EVs of EGFRvIII expressing glioma cells, and downregulation of exosomal markers (CD81 and CD82) relative to EVs of EGFRvIII-negative cells. (cancer-genetics.org)
  • Degeneration of matrix is a key event in invasion and metastasis of malignant lesions of the head and neck. (biomedcentral.com)
  • Nitrite is one of the most common environmental contaminants affecting Extracellular Matrix (ECM) proteins expression. (biomedres.info)
  • Observed changes included increased expression of Fibronectin, Collagen IV, and Laminin V in PIs cultured in the RCCS system. (brighton.ac.uk)
  • PIs digestion with collagenase-accutase enzyme was optimized such that cell viability was not affected but cellular stress and subsequent remodelling of ECM expression was achieved. (brighton.ac.uk)
  • However, the cellular roles of GPR56 have remained unclear. (jneurosci.org)
  • Furthermore, comprehensive analysis of acellular matrix ECM details significant compositional differences between normal and fibrotic lungs, paving the way for further study of novel hypotheses. (atsjournals.org)