He was also the first to show that RuvA and RuvB are motor proteins that mediate Holliday junction branch migration. (wikipedia.org)
Intriguingly, the core complex component FANCM also catalyzes branch migration of model Holliday junctions and replication forks in vitro. (infona.pl)
Migration1
The remodelling of duplex DNA, as well as the migration of fork and Holliday junctions, is a central requirement of HR and is achieved by a number of DNA helicases. (biomedcentral.com)
Pathways2
Present understanding indicates that there are three distinct pathways of Holliday junction processing in human cells involving BLM-topoIIIα-RMI1-RMI2, MUS81-EME1 and GEN1. (wikipedia.org)
Cells have evolved with conserved recombination mediated genome editing pathways as a mean for repairing DSBs and restarting replication forks, thus allowing genome duplication to continue [ 8 ]. (springeropen.com)
Recombination6
During his PhD work, he became interested in how cells recombine their DNA and use recombination for DNA repair. (wikipedia.org)
After moving to the UK in 1985, West continued his work in bacterial systems, and set about trying to identify cellular proteins capable of resolving recombination intermediates. (wikipedia.org)
He identified RuvC as the first cellular enzyme that resolves recombination intermediates and characterised how this nuclease cuts Holliday junctions. (wikipedia.org)
Homologous recombination (HR) is a major DNA repair pathway that may be employed for the repair of double-strand DNA breaks (DSBs) during S and G2 of the cell cycle [ 2 ]. (biomedcentral.com)
DSBs not only ensue from normal cellular metabolism, in the form of reactive oxygen species that can oxidize DNA bases [ 1 , 2 ], but can also be generated during physiological processes like chromosome replication, meiotic recombination and DNA replication transcription collision [ 3 - 7 ]. (springeropen.com)
The double Holliday junction (dHJ) is generally regarded to be a key intermediate of meiotic recombination, whose resolution is critical for the formation of crossover recombinants. (infona.pl)
In addition, BLM is required for the dissolution of double Holliday junctions, during the later stages of HR [ 11 ]. (biomedcentral.com)
Non-homologous end joining (NHEJ), which does not depend upon sequence homology, is the key repair pathway during the G0/G1 stages of the cell cycle [ 10 ]. (springeropen.com)
Enzymes1
Junction resolving enzymes use multivalency to keep the Holliday junction dynamic. (jhmi.edu)
However, erroneous repair of DSBs can lead to chromosomal rearrangements and loss of heterozygosity, which in turn can also cause cancer and cell death. (springeropen.com)
Failure to repair DSBs can lead to unwanted consequences, such as loss of genetic information, chromosomal rearrangements and even cell death. (springeropen.com)
FANCM remodels branched DNA structures and plays essential roles in the cellular response to DNA replication stress. (infona.pl)
Human cells1
Maintenance of genome stability is critical in human cells. (biomedcentral.com)
Gene2
For instance, effective transfer of programmable nucleases to living cells, tissues, or organs that contain the target gene is important for genome editing. (molcells.org)
old transporters Activated at the zone of activation and on elucidated authorities are with membrane cycle, splicing to a polymerization of modifications that conducts with the DNA of an national action activation. (evakoch.com)
Traversing an extraordinarily thin membrane, the cilia of the hair cells are embedded in the tectorial membrane, whose free end is located above the cells. (cloudaccess.net)
The bodies of the cochlear sensory cells resting on the basilar membrane are surrounded by nerve terminals, and their approximately 30,000 axons form the cochlear nerve. (cloudaccess.net)
Genome3
The maintenance of genome stability is an essential requirement for cellular homeostasis. (biomedcentral.com)
For genome correction by introducing target-specific genetic scissors in various organisms, it is necessary to harness the endogenous cellular DNA repair system. (molcells.org)
However, in the midst of this process of genome editing, small deletions and insertions might be introduced at the junction site. (springeropen.com)
Degradative1
IL23 aims degradative and known in different similar cellular collagens clock-wise as aciduria, Edition, protein and methionine. (evakoch.com)
Protein4
In the Howard-Flanders group at Yale University, West purified and characterised RecA protein, and in doing so discovered many key aspects relating to the way that cells mediate DNA-DNA interactions and strand exchange. (wikipedia.org)
In addition to the discovery of cellular Holliday junction resolvases, West was the first to purify the human RAD51 protein (the eukaryotic ortholog of RecA), and to show that it promotes homologous pairing and strand exchange reactions similar to those mediated by RecA. (wikipedia.org)
We show that hSSB1 depleted cells contain less BLM protein and that this deficiency is due to proteasome mediated degradation of BLM. (biomedcentral.com)
Our data highlights that BLM helicase and hSSB1 function in a dynamic complex in cells and that this complex is likely required for BLM protein stability and function. (biomedcentral.com)
Organisms3
HDR is mainly activated in the S/G2 phase of the cell cycle in higher organisms. (molcells.org)
On a cellular level, we look at the evolution of epithelia in the most primitive metazoan organisms (sea-water sponges) as an important structure to define organismal and tissue boundaries (in collaboration with Andre le Bivic and Carole Borchiellini). (univ-amu.fr)
Cells of all living organisms have evolved complex mechanisms that serve to stabilise, repair and restart stalled, blocked and broken replication forks. (infona.pl)
Intact1
Enzymatic processing of the intervening junctions (Holliday junctions) subsequently yields two intact dsDNA strands. (biomedcentral.com)
Ionising radiation2
In this study, we demonstrate that hSSB1 and BLM helicase form a complex in cells and the interaction is altered in response to ionising radiation (IR). (biomedcentral.com)
Abstract Caffeine potentiates the lethal effects of ultraviolet and ionising radiation on wild-type Schizosaccharomyces pombe cells. (infona.pl)
Form1
All auditory information is transduced by only 15,000 hair cells (organ of Corti), of which the so-called inner hair cells, numbering 3,500, are critically important, since they form synapses with approximately 90% of the 30,000 primary auditory neurons (figure 2). (cloudaccess.net)
Transfer1
These human RNAs Do a removing download Ethos and see cities, overhangs, cells, ATPases operators, 7SL RNA, 7SK RNA, the RNA cell of RNase transfer RNA, the RNA number of RNase MRP, and the RNA molecule of conservation. (evakoch.com)
Complex1
Strategy for Compositional Analysis of the Hair Cell Mechanotransduction Complex Using TIRF Microscopy. (jhmi.edu)
Intermediates2
After moving to the UK in 1985, West continued his work in bacterial systems, and set about trying to identify cellular proteins capable of resolving recombination intermediates. (wikipedia.org)
He identified RuvC as the first cellular enzyme that resolves recombination intermediates and characterised how this nuclease cuts Holliday junctions. (wikipedia.org)
Somatic cells1
Yet contrary to Dmc1, Rdh54/Tid1 is also present in somatic cells where its function is less understood. (elifesciences.org)
Strand3
In the Howard-Flanders group at Yale University, West purified and characterised RecA protein, and in doing so discovered many key aspects relating to the way that cells mediate DNA-DNA interactions and strand exchange. (wikipedia.org)
In addition to the discovery of cellular Holliday junction resolvases, West was the first to purify the human RAD51 protein (the eukaryotic ortholog of RecA), and to show that it promotes homologous pairing and strand exchange reactions similar to those mediated by RecA. (wikipedia.org)
The D-loop thus features a displaced ssDNA, a Rad51-free hDNA and DNA strand exchange junctions at both extremities of the hDNA. (elifesciences.org)
Biosciences1
Jucker Award 2015 for contributions to cancer research and in 2017 the Friedrich Miescher Award from the LS2 section of Molecular and Cellular Biosciences. (usi.ch)
Proteins4
He was also the first to show that RuvA and RuvB are motor proteins that mediate Holliday junction branch migration. (wikipedia.org)
It cleaves the membrane-bound precursor of TUMOR NECROSIS FACTOR-ALPHA between ALANINE 76 and VALINE 77 to its functional form, as well as several other CELL SURFACE PROTEINS to their soluble forms, including AMYLOID BETA-PROTEIN PRECURSOR and PRION PROTEIN. (nih.gov)
HN - 2017 MH - ADAM12 Protein UI - D000072199 MN - D8.811.277.656.675.374.102.125 MN - D9.400.430.500.125 MN - D12.776.395.33.125 MS - A disintegrin and metalloproteinase domain-containing protein that is expressed as two alternatively-spliced forms: a long transmembrane form (ADAM12-L) and a short soluble form (ADAM12-S). It modulates the cleavage of INSULIN-LIKE GROWTH FACTOR BINDING PROTEINS and may also regulate CELL FUSION during MYOGENESIS. (nih.gov)
In contrast to ADAM proteins, which reside on CELL MEMBRANES, ADAMTS proteases are secreted and function in the EXTRACELLULAR MATRIX. (nih.gov)
Laboratory1
His laboratory discovered that the Holliday junction resolvase activities of MUS81 and GEN1 are regulated so that they act late in the cell cycle to ensure chromosome segregation. (wikipedia.org)
Regulates1
Specifically, it regulates the organization of apical plasma membrane in epithelial cells, disk morphogenesis during early retinal development, MAPK and Akt signaling pathways, and in cholesterol metabolism. (nih.gov)
Human4
Present understanding indicates that there are three distinct pathways of Holliday junction processing in human cells involving BLM-topoIIIα-RMI1-RMI2, MUS81-EME1 and GEN1. (wikipedia.org)
MH - A549 Cells UI - D000072283 MN - A11.251.210.190.80 MN - A11.251.860.180.80 MN - A11.436.54 MS - An immortalized cell line derived from human ADENOCARCINOMA, ALVEOLAR basal epithelial cells isolated from the lungs of a male patient in 1972. (nih.gov)
In humans, it is expressed as a subset of CD34 (bright) human hematopoietic stem cells and CD34 positive leukemias. (nih.gov)
During his PhD studies with Prof. Josef Jiricny, Petr Cejka studied the function of the mismatch repair system in human cells, as well as how mismatch repair mediates the sensitivity of DNA methylating agents used in anti-cancer therapy. (usi.ch)