• Cell 100 , 57-70 (2000). (nature.com)
  • 2000 ). The encapsulation of cells has been developed to avoid immune rejection in cell/tissue transplantation. (springer.com)
  • Based on high-resolution microscopic images, high content analysis (HCA) enables you to detect cellular phenotypic changes at a molecular level, so the technology can be utilized as a powerful tool to study effects of physiologically active substances, such as compounds, siRNA, peptides and antibodies, on cultured cells, tissue samples and whole organisms, like zebrafish. (yokogawa.com)
  • thus, transcription errors represent a new molecular mechanism by which cells can acquire disease phenotypes. (nih.gov)
  • The findings reinforce the importance of screening iPSCs for potentially harmful DNA mutations before using them for therapeutic purposes, said lead investigators Ali Torkamani, Ph.D., director of genome informatics at STSI, and Kristin Baldwin Ph.D., the study's co-lead investigators and associate professor of molecular and cellular neuroscience at the Dorris Neuroscience Center at TSRI. (sciencedaily.com)
  • Proteins must be folded correctly to fulfill their molecular functions in cells. (mpg.de)
  • Newly-synthesized proteins at the ribosomes are not yet able to fulfill their molecular functions in the cell. (mpg.de)
  • Mechanical stress can modulate physiological processes at the molecular, cellular and systemic level. (springer.com)
  • The topics are discussed in a form of review articles, written by leading researchers who study the mechanosensitivity process at the molecular, cellular, tissue and whole organ levels. (springer.com)
  • Comparative molecular portraits of human unfertilized oocytes and primordial germ cells at 10 weeks of gestation. (mpg.de)
  • Over the last few decades, the cellular and molecular basis for many host defense functions has been uncovered through the use of emerging laboratoty technologies. (cdc.gov)
  • Paul, 1992), it discusses many of the cellular and molecular components of the immune system and their use as biologic markers. (cdc.gov)
  • By combining eight different areas of expertise (Proteomics, Genomics, Targeted Therapy, Molecular Diagnostics, Chemical Biology, Stromal Biology, psychological Resilience and Cancer Stem Cells), we will come one step closer to conquering cancer. (lu.se)
  • In a study published in the Molecular Cell journal, they explain what makes hematopoietic stem cells acquire malignant traits in cancer. (lu.se)
  • In recent years there have been significant advances in our understanding of the cellular and molecular mechanisms through which allergic sensitization to chemicals is induced and regulated. (cdc.gov)
  • Cellular senescence is a multifaceted process that arrests the proliferation of cells that are at risk of neoplastic transformation. (nature.com)
  • Jurkat T cells encapsulated with a double layer of PSS-GNR/PAH (PSS-GNR/PAH@Jurkat) showed the highest rate of cell proliferation when compared to 24-h encapsulated cells. (springer.com)
  • Curcumin, a natural polyphenolic compound derived from turmeric (Curcuma longa L), has proven to be a modulator of multiple intercellular signalling pathways linked to inflammation, to proliferation, growth, invasion, drug sensitivity, angiogenesis and metastasis of cancer cells. (researchgate.net)
  • The protein produced from the normal ABL1 gene is involved in many cellular processes, including cell growth and division (proliferation), maturation (differentiation), movement (migration), and self-destruction (apoptosis). (medlineplus.gov)
  • Like the ABL1 protein, the abnormal protein produced from the fusion gene, called BCR-ABL1, can promote cell proliferation and block apoptosis. (medlineplus.gov)
  • However, potentially erroneous characterization of cytotoxicity and cell proliferation can arise if verification of baseline interaction of test article with method employed is neglected. (cdc.gov)
  • Non-tumorigenic Beas-2B cells were treated with graded concentrations of benzo[a]pyrene (B[a]P) for 24 and 48h prior to cytotoxicity and proliferation assessment with commonly used MTT, MTS, WST1, and Alamar Blue assays. (cdc.gov)
  • These results demonstrate differential sensitivity of standard cytotoxicity assessments on the PPP, thus (1) decoupling 'mitochondrial activity' as an interpretation of cellular formazan and Alamar Blue metabolism, and (2) demonstrating the implicit requirement for investigators to sufficiently verify interaction of these methods in routine cytotoxicity and proliferation characterization. (cdc.gov)
  • Because FCDI's MyCell Products are created using custom iPS cell reprogramming and differentiation processes, they provide biologically relevant human cells from donors with specific disease-associated genotypes and phenotypes. (wikipedia.org)
  • Understanding the causes and consequences of cellular senescence has provided novel insights into how cells react to stress, especially genotoxic stress, and how this cellular response can affect complex organismal processes such as the development of cancer and ageing. (nature.com)
  • To further investigate the physiological processes 40 underlying GBM cell movement, this study aimed to image and analyze distinct GBM invasive growth patterns found in vivo , similar to those observed in patients. (nature.com)
  • Adipose tissue-derived mesenchymal stem cells (AD-MSC) show great potential to treat inflammatory and degradative processes in OA and have demonstrated paracrine effects in chondrocytes. (karger.com)
  • however, germline genetic variants play a role in tumorigenesis by partaking in critical biological and cellular processes. (hindawi.com)
  • Actin is the most abundant protein in highly developed cells and has diverse functions in processes like cell stabilization, cell division and muscle contractions. (mpg.de)
  • Thus, acting on ion channels in the plasma membrane, mechanical stress can elicit a multitude of biochemical processes - both transient and long-lasting - inside a cell. (springer.com)
  • Treated cells undergo a remarkable shift in structure, acquiring an astrocyte-like morphology with star-shaped bodies and radial branched processes. (lu.se)
  • We take advantage of the similarity between neural developmental processes and cancer hierarchy to mitigate, if not completely abolish, the malignant nature of tumor cells and pave the way for new intervention strategies. (lu.se)
  • This simplified approach towards understanding the essence of the mechanisms, underlying the processes determining life and death of a cell has undoubtedly provided scientists with enormous amount of knowledge. (intechopen.com)
  • Evidence suggests that surface-sensing triggers transcriptional regulation as well as post-translational signaling that promote a variety of interesting cell phenotypes. (aps.org)
  • Studying cellular phenotypes is very dependent on the dynamics and functionality of imaging tools at an optimum or temporal resolution. (essaywriter.org)
  • New technologies have advanced both hardware and software for automated imagery analysis which allows researchers to screen images without limitation of the cell quantity being screened (Usaj et al, 2016). (essaywriter.org)
  • Several published studies have used FCDI products to investigate mechanisms of toxicity and have leveraged cellular models to identify toxicity earlier in the drug development process. (wikipedia.org)
  • Using a combination of modeling, conceptual theory, and experiments, we show that multiple, redundant self-organization mechanisms coexist within the protein network underlying cell polarization and are responsible for the module's resilience and adaptability. (biorxiv.org)
  • This is in part because the E. coli have cellular mechanisms that prevent single-stranded DNA from being accumulated and integrated into their genomes. (scienceblog.com)
  • Human induced pluripotent stem cells--from mechanisms to clinical applications. (mpg.de)
  • These functions are carried out through both nonspecific mechanisms of innate or natural immunity and through specific mechanisms of acquired immunity that develops as the organism encounters environmental agents or antigens. (cdc.gov)
  • In this study, we investigated programmed death-ligand 1 (PDL-1) as a potential biomarker for CAF-induced chemoresistance and evaluated its role and the underlying mechanisms of chemoresistance in non-small cell lung cancer (NSCLC). (cdc.gov)
  • FCDI's iCell and MyCell products are being used to research the development of regenerative medicine approaches, including: regenerative medicine compound screening, allogeneic and autologous cell therapy, tissue engineering, and transplantation. (wikipedia.org)
  • Want to analyze cellular or tissue samples from experiments performed in the lab? (stu.edu)
  • Therefore, in addition to epidermal Langerhans cells, other potential cellular origins for LCH include dermal langerin + dendritic cells, lymphoid tissue-resident langerin + dendritic cells, and monocytes that can be induced by local environmental stimuli to acquire a Langerhans cell phenotype. (medscape.com)
  • The primary functions of the host defense system are repairing injured tissue, identifying and removing foreign substances, destroying or containing infectious agents, and, in some cases, eradicating cancer cells. (cdc.gov)
  • Confocal laser scanning microscopy is mainly used in biomedical sciences for high-resolution analysis of cell and tissue structures labeled with fluorescent markers. (lu.se)
  • Consequently, we aimed to assess miR-637 expression in gliomas to determine the effects of miR-637 on glioma cell migration and invasion, as well as to explore the possible signaling pathways involved. (scirp.org)
  • Furthermore, we investigated the cellular pathways that are involved, and these are very fundamental for differentiating these cells to the placental phenotype (i.e., appearance and gene signature). (ca.gov)
  • There is now substantial evidence that cellular senescence is a barrier to malignant tumorigenesis in vivo . (nature.com)
  • In mammalian organisms, cells that express markers of senescence have been shown to accumulate with age and at sites of certain age-related pathologies. (nature.com)
  • There is also mounting evidence that cellular senescence contributes to ageing. (nature.com)
  • Proliferating cells can initiate an additional response by adopting a state of permanent cell-cycle arrest that is termed cellular senescence. (nature.com)
  • Campisi, J. Cellular senescence as a tumor-suppressor mechanism. (nature.com)
  • They are, for instance, active in senescence, acting to transform non-senescent cells to senescent status. (virology.ws)
  • Langerhans cell histiocytosis (LCH) is a group of idiopathic disorders characterized by the presence of cells with characteristics similar to bone marrow-derived Langerhans cells juxtaposed against a backdrop of hematopoietic cells, including T-cells, macrophages, and eosinophils. (medscape.com)
  • While a few studies have attempted reprogramming of glioblastoma (GBM) cells toward a neuronal fate, this approach has not yet been used to induce differentiation into other lineages and in vivo data on reduction in tumorigenicity are limited. (lu.se)
  • Here, we employ cellular reprogramming to induce astrocytic differentiation as a therapeutic approach in GBM. (lu.se)
  • Most importantly, forced differentiation substantially reduces tumorigenicity of GBM cells in an in vivo xenotransplantation model. (lu.se)
  • The CellVoyager CQ1 provides the highest quality confocal images and extended live-cell imaging in a space-saving benchtop design. (yokogawa.com)
  • Our high-content confocal imaging systems based on the CSU are the ideal solution for drug screening using elaborated 3D and live cell assay systems as well as 2D fixed samples. (yokogawa.com)
  • In SK-N-SH cells, carbachol induced a rapid and transient translocation of tubulin to the plasma membrane, microtubule reorganization, and a change in cell shape as demonstrated by confocal immunofluorescence microscopy. (jneurosci.org)
  • The new microscope is based on confocal microscopy, a technique commonly used for cell imaging. (news-medical.net)
  • The difficulty in quantitatively acquiring cellular morphological and mechanical information in vivo makes mathematical modeling a challenging problem and impedes model validation. (aps.org)
  • In vivo experiments were conducted using a co-implantation xenograft mouse model with live cell imaging and immunohistochemistry. (cdc.gov)
  • The working group of the Histiocyte Society divided histocytic disorders into three groups: (1) dendritic cell histiocytosis, (2) macrophage-related disorders, and (3) malignant histiocytosis. (medscape.com)
  • References 2 and 3 describe the characteristics of cancer cells and the importance of mutations in cancer development. (nature.com)
  • Researchers who looked at the effect of aging on induced pluripotent stem cells (iPSCs) found that genetic mutations increased with the age of the donor who provided the source cells, according to study results. (sciencedaily.com)
  • Researchers at the Scripps Translational Science Institute (STSI) and The Scripps Research Institute (TSRI) who looked at the effect of aging on induced pluripotent stem cells (iPSCs) found that genetic mutations increased with the age of the donor who provided the source cells, according to study results published by the journal Nature Biotechnology . (sciencedaily.com)
  • Our study highlights that increased risk of mutations in iPSCs made from older donors of source cells. (sciencedaily.com)
  • Researchers found that iPSCs made from donors in their late 80s had twice as many mutations among protein-encoding genes as stem cells made from donors in their early 20s. (sciencedaily.com)
  • Unexpectedly, iPSCs made from blood cells donated by people over 90 years old actually contained fewer mutations than what researchers had expected. (sciencedaily.com)
  • Researchers said the reason for this could be tied to the fact that blood stem cells remaining in elderly people have been protected from mutations over their lifetime by dividing less frequently. (sciencedaily.com)
  • Of the 336 different mutations that were identified in the iPSCs generated for the study, 24 were in genes that could impair cell function or trigger tumor growth if they malfunctioned. (sciencedaily.com)
  • How troublesome these mutations could be depends on how well the stem cells are screened to filter out the defects and how they are used therapeutically, Torkamani said. (sciencedaily.com)
  • High-throughput genotyping and next-generation sequencing technologies have enabled discovery of genetic risk variants and acquired somatic mutations driving the disease. (hindawi.com)
  • For decades, germline mutations, contained within the heritable genome, and somatic mutations, acquired de novo by breast cancer cells, have been considered as separate research endeavours, and each has unique clinical applications and implications for patient care. (hindawi.com)
  • Cancer is not one disease, but hundreds, that develops when multiple mutations in a cell result in escape of the cellular proofreading system. (lu.se)
  • Splicing factor mutations are particularly prevalent in MDS, a group of heterogeneous hematological disorders characterized by defective blood stem cells and a high risk of leukemia development. (lu.se)
  • Hayflick, L. The limited in vitro lifetime of human diploid cell strains. (nature.com)
  • A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
  • Although other irritants in the smoke may have contributed to the incident, there is supporting evidence that stable strontium can stimulate the release of histamine from mast cells in vitro (ATSDR 2001e). (cdc.gov)
  • Described here are some of the general requirements of in vitro test methods for skin sensitization, and progress that has been made in developing suitable approaches with particular emphasis on the utility of dendritic cell culture systems. (cdc.gov)
  • This is an acquired immune system, which has two components -- non-cellular (humoral) and cellular components. (kemin.com)
  • The course deals with the autogenous immune system, immune competence, humoral and cellular immunity. (uib.no)
  • The term "immune system" will be used to refer to all components of nonspecific innate immunity and antigen-specific acquired immunity, since their components and activities are invariably intertwined. (cdc.gov)
  • Researchers can reprogram diseased cells of interest to study how a particular disease affects those cell types and to discover methods of repairing the cells. (wikipedia.org)
  • The new DNA writing technique, which the researchers call HiSCRIBE, is much more efficient than previously developed systems for editing DNA in bacteria, which had a success rate of only about 1 in 10,000 cells per generation. (scienceblog.com)
  • In a new study, the researchers demonstrated that this approach could be used for storing memory of cellular interactions or spatial location. (scienceblog.com)
  • To achieve that, the researchers engineered the cells to produce a reverse transcriptase enzyme called retron, which produces a single-stranded DNA (ssDNA) when expressed in the cells, and a recombinase enzyme, which can insert ("write") a specific sequence of single-stranded DNA into a targeted site in the genome. (scienceblog.com)
  • In each generation, out of 10,000 E. coli cells, only one would acquire the new DNA that the researchers tried to incorporate into the cells. (scienceblog.com)
  • By integrating a DNA "barcode" into each cell's genome, which can then be exchanged with other cells, the researchers can determine which cells have interacted with each other by sequencing their DNA to see which barcodes they carry. (scienceblog.com)
  • Researchers at the Max Planck Institute of Biochemistry (MPIB) have demonstrated that actin, the most abundant protein in higher developed cells, does not have the inbuilt potential to fold and instead requires special assistance to fold into its active state. (mpg.de)
  • Researchers from the department "Cellular Biochemistry" of MPIB director F.-Ulrich Hartl have now revealed the unique non-folding properties of the universal protein actin. (mpg.de)
  • In comparison with similar proteins that build the cytoskeleton of bacteria, which are evolutionary older and simpler cells, the researchers found fundamental differences in the protein folding. (mpg.de)
  • Direct cellular reprogramming has recently gained attention of cancer researchers for the possibility to convert undifferentiated cancer cells into more differentiated, postmitotic cell types. (lu.se)
  • Researchers visually assessed cell plating densities of the chambers after each experiment came to an end. (essaywriter.org)
  • Researchers incubated HeLa Kyoto cells for about 50hours on the siRNA arrays and then fixed them with paraformaldehyde or methanol. (essaywriter.org)
  • This allowed the researchers to measure distances for all experimental cells from the control cells. (essaywriter.org)
  • A combination of automated fluorescence and quantitative imagery enables researchers to acquire unbiased multipara-metric information down at a single cells' level. (essaywriter.org)
  • Researchers have developed a microscope specifically for imaging large groups of interacting cells in their natural environments. (news-medical.net)
  • To acquire multiple planes simultaneously, the researchers developed a way to reuse the light for imaging cells in one plane to also image cells deeper in the sample. (news-medical.net)
  • A team of researchers led by Dr. Cristian Bellodi recently discovered a hardwired genetic control mechanism modulating individual spliceosomal components, known as splicing factors, in cells harboring oncogenic lesions common in human cancers. (lu.se)
  • We have previously discussed the idea that viruses originated from selfish genetic elements such as plasmids and transposons when these nucleic acids acquired structural proteins (see A plasmid on the road to becoming a virus ). (virology.ws)
  • Missing from these hypothesis is how nucleic acids became virus particles - that is, how they acquired structural proteins. (virology.ws)
  • Self-replicating, virus like nucleic acids emerged in the pre-cellular world and from the emerged the first cells. (virology.ws)
  • The replicating nucleic acids entered the cells, where they replicated and became genetic parasites. (virology.ws)
  • Develop a biological knowledge base common to contemporary biologists, encompassing microscopy, cell structure, cellular membranes, and nucleic acids biology. (uaeu.ac.ae)
  • We found an interesting array of factors that are regulated, and we are now poised to study human endometrial epithelial cells (not a cell line), since we know that a response occurs in these pilot studies with a cell line. (ca.gov)
  • In H. pylori , we have been investigating the geometric localization of the proteins and metabolic probes of cell wall growth. (aps.org)
  • Consistent with these labels, two of the proteins associated with cell wall homeostasis show geometric localization. (aps.org)
  • Like all proteins, actin is "born" at the ribosomes, the protein factories of the cell, which string together amino acids into a long chain. (mpg.de)
  • Misfolded proteins can aggregate into toxic clumps, which damage cells and contribute to Alzheimer's and Parkinson's disease. (mpg.de)
  • Therefore, even though proteins can also fold in their absence, chaperones contribute to cellular health by improving the folding efficiency. (mpg.de)
  • Unlike all previously studied proteins, actin cannot acquire its fold in absence of the chaperonin TRiC. (mpg.de)
  • I want to explore in more detail the idea that the structural proteins of viruses likely originated from cell proteins ( link to paper ). (virology.ws)
  • It seems likely that viral structural proteins originated from cellular genes. (virology.ws)
  • An analysis of the sequence an structure of major virion proteins has identified likely ancestors in cellular proteins. (virology.ws)
  • Many cell proteins have jelly role motifs, and some form 60-subunit virus-like particles in cells. (virology.ws)
  • The extra sequences at the N-termini of viral jelly roll capsid proteins, involved in recognizing the viral genome, likely evolved after the capture of these proteins from cells. (virology.ws)
  • Retroviral structural proteins also appear to have originated from cell proteins, with clear homologies with matrix, capsid, and nucleocapsid proteins. (virology.ws)
  • The matrix Z proteins of arenaviruses are related to cellular RING domain proteins, and the matrix proteins of some negative strand RNA viruses are related to cellular cyclophilin. (virology.ws)
  • There are many more examples, providing support for the hypothesis that viruses evolved on multiple instances by recruiting different cell proteins. (virology.ws)
  • At some point these genetic elements acquired structural proteins from the cells and became bona fide virus particles. (virology.ws)
  • We know that viral proteins can be returned to cells, where they serve useful functions. (virology.ws)
  • Intrinsic antiviral resistance is a branch of antiviral defence that involves constitutively expressed cellular proteins that act within individual infected cells. (mdpi.com)
  • This editorial highlights the relevance of interfering in cancer cell progression through the pharmacological manipulation on the cell metabolism of cyclic nucleotides such as cAMP, and on the intracellular Ca2+ signaling, which may avail the reduction of toxic effects promoted by chemotherapy, radiotherapy and immunotherapy, thus decreasing the incidence of interruption in antitumoral treatment. (researchgate.net)
  • Our computational imaging framework allows us to extract 3D shapes of individual bacterial cells from fluorescence microscopy images. (aps.org)
  • Fast image acquisition was important because the fluorescence indicators that monitor cellular function typically operate on time scales of a few tens of milliseconds. (news-medical.net)
  • As it is in much of life, the aging process isn't kind to an important type of stem cell that has great therapeutic promise. (sciencedaily.com)
  • To this end, we overexpressed key transcriptional regulators of astroglial development in human GBM and GBM stem cell lines. (lu.se)
  • Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
  • We have found that these cells initially lose their stem cell characteristics and change their appearance from stem cells to trophoblast (placental) cells. (ca.gov)
  • RESULTS: We demonstrated that chemotherapy-stimulated CAFs promoted tumorigenic and stem cell-like properties of NSCLC cells, which contribute to their chemoresistance. (cdc.gov)
  • Silencing PDL-1 expression suppressed CAFs' ability to promote stem cell-like properties and invasiveness of lung cancer cells, favoring chemoresistance. (cdc.gov)
  • CONCLUSION: Our results show that PDL-1-positive CAFs modulate stem cell-like properties of NSCLC cells by secreting elevated HGF, thereby promoting chemoresistance. (cdc.gov)
  • It is suggested that G-protein-coupled receptors might modulate cytoskeletal dynamics, intracellular traffic, and cellular architecture. (jneurosci.org)
  • Notably, LCH cells have been found to express markers of both resting epidermal Langerhans cells (CD1a, intracellular major histocompatibility complex II [MHCII], Birbeck granules) and activated Langerhans cells (including CD54 and CD58). (medscape.com)
  • There is also the phenomenon of HAP2 - a fusion protein that is used by many different eucaryotic organisms "all over" the phylogenetic tree (from Chlamydomonas to Bees) for mediating cell-cell fusion, predominantley of gametes. (virology.ws)
  • Now, the question if of course - is it of viral origin or is it a cellular protein co-opted by viruses? (virology.ws)
  • A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged TgCentrin2 and mCherry-tagged TgIMC1 (innermembrane complex scaffold protein). (cellimagelibrary.org)
  • The function of the protein produced from the normal BCR gene is not completely understood, although it has been shown to help control signaling in cells. (medlineplus.gov)
  • The constantly active BCR-ABL1 protein signals cells to continue dividing abnormally and prevents them from self-destructing, which leads to overproduction of the abnormal cells and, eventually, a shortage of normal blood cells. (medlineplus.gov)
  • Prion diseases result from misfolding of a normal cell-surface brain protein called cellular prion protein (PrP C ), whose exact function is unknown. (msdmanuals.com)
  • Cellular Physiology and Biochemistry (2018) 47 (1): 11-25. (karger.com)
  • 4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
  • These experiments reveal a previously unappreciated role for transcriptional fidelity in cellular health and aging. (nih.gov)
  • A transcriptional roadmap to the induction of pluripotency in somatic cells. (mpg.de)
  • Preparation of mouse embryonic fibroblast cells suitable for culturing human embryonic and induced pluripotent stem cells. (mpg.de)
  • A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged Tgbeta3-tubulin. (cellimagelibrary.org)
  • A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing YFP-tagged Tgalpha1tubulin and mRFP-tagged TgDLC (dynein light chain). (cellimagelibrary.org)
  • Biological engineers at MIT have devised a new way to efficiently edit bacterial genomes and program memories into bacterial cells by rewriting their DNA. (scienceblog.com)
  • The organization of bacterial cells is less complex than in higher developed cells. (mpg.de)
  • Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)
  • 8 Department of Cell Biology and Physiology, University of North Carolina, Chapel Hill, North Carolina 27599, USA. (nih.gov)
  • To make progress in answering these basic questions of evolutionary cell biology, we analyze, as a concrete example, the cell polarity machinery of Saccharomyces cerevisiae . (biorxiv.org)
  • Define the basis of eukaryotic cell biology. (uaeu.ac.ae)
  • The course is intended to complement Basic Biology I (which covers cellular and biochemical basis of life) by covering the basic concepts relating to whole-organism topics. (uaeu.ac.ae)
  • moreover, normal cell regulation can be disrupted by tumor-suppressive or oncogenic miRNAs. (scirp.org)
  • RNA splicing is a major nexus of gene expression regulation, shaping cellular identity during development, frequently altered in human cancers. (lu.se)
  • Fujifilm Cellular Dynamics, Inc. (FCDI) is a large scale manufacturer of human cells, created from induced pluripotent stem cells, for use in basic research, drug discovery and regenerative medicine applications. (wikipedia.org)
  • Prior to acquisition, the company then named Cellular Dynamics International, Inc. tendered an initial public offering in July 2013 under the ticker symbol ICEL. (wikipedia.org)
  • On March 30, 2015, Fujifilm Holdings Corporation announced an agreement to acquire Cellular Dynamics International for $307 million or $16.50/share on a fully diluted basis. (wikipedia.org)
  • The company was subsequently renamed Fujifilm Cellular Dynamics, Inc. Human cells are considered to be a more predictive model and a replacement for other cellular models that serve as proxies, including animal cells, immortalized cell lines, and cadaveric cells. (wikipedia.org)
  • Tumor cell dynamics at different border configurations were analyzed and multivariate linear modelling of tumor cell spreading was performed. (nature.com)
  • If a similar approach could be deployed in mammalian cells, it could someday be used to map interactions between other types of cells such as neurons, Farzadfard says. (scienceblog.com)
  • The hybridization images were acquired under a fluorescent microscope. (scirp.org)
  • Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. (cellimagelibrary.org)
  • It is the prevailing microscope technique for "deeper" analysis at cellular and sub-cellular levels. (lu.se)
  • As a result, the pathologic cells of LCH have been hypothesized to represent Langerhans cells in a state of arrested maturation. (medscape.com)
  • Normal PrP C is water soluble and protease sensitive, but a large percentage of PrP Sc is water insoluble and markedly resistant to protease degradation (similar to beta-amyloid in Alzheimer disease, which PrP Sc resembles), resulting in slow but inexorable cellular accumulation and neuronal cell death. (msdmanuals.com)
  • Yokogawa CSU is renowned in microscopy field not only for the high image quality and fast capturing speed, but also for the live cell friendliness. (yokogawa.com)
  • Our dedicated histological and microscopy core enables students to analyze the finest detailed structures within cells and tissues. (stu.edu)
  • The major theme will be devoted to describe and analyze the molecules of life, light microscopy, fine cell structure and function, cell membranes structure and function, cell division and staining procedures. (uaeu.ac.ae)
  • Active immunity can be acquired in two ways, either by contracting the disease or through vaccination. (cdc.gov)
  • We found that they lose their stemness and acquire genes important for commitment to be placental cells. (ca.gov)
  • Mechanistically, an upregulation of PDL-1 in chemotherapy-treated CAFs led to an increase in hepatocyte growth factor (HGF) secretion, which stimulates cancer progression, cell invasion, and stemness of lung cancer cells, while inhibiting apoptosis. (cdc.gov)
  • Specifically, a variety of other cellular populations have been identified that possess phenotypic characteristics similar to Langerhans cells, including expression of CD207 and Birbeck granules. (medscape.com)
  • Primary brain tumor initiating cell lines from IDH-wild type GBM stably expressing H2B-Dendra2 were implanted orthotopically in the brains of SCID mice. (nature.com)
  • Using high-resolution time-lapse intravital imaging, tumor cell migration in the tumor core, border and invasive front was recorded. (nature.com)
  • Not only tumor borders but also the tumor core was composed of highly dynamic cells, with no clear correlation to the ability to spread into the brain. (nature.com)
  • Two types of border configurations contributed to tumor cell spreading through distinct invasion patterns: an invasive margin that executes slow but directed invasion, and a diffuse infiltration margin with fast but less directed movement. (nature.com)
  • The improved built-in incubator lets you analyze extended live cell responses. (yokogawa.com)
  • A specific and time-dependent association of tubulin with plasma membranes was observed when SK-N-SH cells were treated with carbachol. (jneurosci.org)
  • The same phenomenon was observed with membranes from Sf9 cells, expressing a recombinant PLCĪ² 1 cascade. (jneurosci.org)
  • Cells continually experience stress and damage from exogenous and endogenous sources, and their responses range from complete recovery to cell death. (nature.com)
  • The laboratory course provides insight into the isolation and study of fish immune cells, selected cell markings, functional analysis and measurement of appropriate immune responses in fish. (uib.no)
  • The transplantation of cells or tissues has been applied for treating various human diseases (Bhatia et al. (springer.com)
  • This book series, Mechanosensitivity in Cells and Tissues, is devoted to the specific response of cells and tissues to mechanical stimulation and has been intensively studied during recent years. (springer.com)
  • The mammalian host defense system is a complex network of cells and mediators with recognition and response functions throughout most tissues of higher organisms. (cdc.gov)
  • Monolayer fixed cell samples have been commonly used in drug screening. (yokogawa.com)
  • For example, cells made from iPSCs for a bone marrow transplant would be potentially dangerous if they contained a TET2 gene mutation linked to blood cancer, which surfaced during the study. (sciencedaily.com)
  • The LARGE principle of cellular reprogramming: lost, acquired and retained gene expression in foreskin and amniotic fluid-derived human iPS cells. (mpg.de)
  • Also, we are investigating whether another cell line has similar characteristics, and we are comparing all the genes expressed in the trophoectomer shell surrounding a human embryo (blastocyst) with the gene profiles in the trophoblast cells derived from human embryonic stem cells. (ca.gov)
  • Role of PDL-1 in NSCLC chemoresistance was assessed using CRISPR/Cas9 knockdown and various functional assays including MTT, cell invasion, sphere formation, and cell apoptosis. (cdc.gov)
  • Next, the effects of miR-637 on glioma cell migration and invasion were determined by using the transwell assay. (scirp.org)
  • In addition, miR-637 suppressed glioma cell migration and invasion. (scirp.org)
  • Human glioma cell lines, U373 and U251 cells, were purchased from the China Academia Sinica Cell Repository (Shanghai, China) and cultured in Dulbecco's Modified Eagle Medium (Gibco) that contained 10% fetal bovine serum (Gibco). (scirp.org)
  • 3. viruses are collections of genes that escaped from cells. (virology.ws)
  • It is important to point out that the genes do not always flow from cells to viruses. (virology.ws)
  • In addition, we conducted a study over 12 days in which these cells exhibited these changes, and we looked at not only their appearance, but also all the genes that they express, using whole genome array technology. (ca.gov)
  • Fluorescent, metabolic probes incorporated into the cell wall show enrichment of growth at both the outer and inner axes. (aps.org)
  • Embryonic development is a precise and complex process involving the cell morphology and mechanics interacting in space and time. (aps.org)
  • In recent years it has been discovered that components of cellular nuclear structures known as ND10 or PML nuclear bodies contribute to intrinsic resistance against a variety of viruses, notably of the herpesvirus family. (mdpi.com)
  • The acquired images can be used for analysis and processing, e.g. 3D-reconstruction, of the labeled structures. (lu.se)
  • FCDI is actively engaged in a number of large-scale iPS cell reprogramming and banking projects, with the goal of creating broadly available resources of iPS cells that represent normal human diversity, disease states and adverse drug reactions. (wikipedia.org)
  • A classic paper that describes the limited replicative lifespan of normal human cells. (nature.com)
  • 8] when using sequence analysis of human colorectal cells. (scirp.org)
  • The helical-rod shape of Helicobacter pylori, a human stomach pathogen, is important for its pathogenesis and is an ideal model system for studying complex bacterial cell shapes. (aps.org)
  • In the present work, we have isolated and characterized the extracellular vesicles from human AD-MSC to investigate their role in the chondroprotective actions of these cells. (karger.com)
  • Currently, human T cell therapy is of considerable scientific interest. (springer.com)
  • Here, we propose an innovative technique of single-cell encapsulation of human T cells using polyelectrolytes combined with gold nanorods. (springer.com)
  • We have demonstrated encapsulation of human Jurkat T cells with poly(sodium 4-styrenesulfonate) (PSS)-coated gold nanorods (PSS-GNRs). (springer.com)
  • Since the 1982 definition was published, human T-cell lymphotropic virus type III/lymphadenopathy-associated virus (HTLV-III/LAV) has been recognized as the cause of AIDS. (cdc.gov)
  • Valproic acid confers functional pluripotency to human amniotic fluid stem cells in a transgene-free approach. (mpg.de)
  • Viral and non-viral generation and characterization of induced pluripotent stem cells from human amniotic fluid cells. (mpg.de)
  • During the past reporting period, we have treated two different lines of human embryonic stem cells with a growth factor, BMP4, and differentiated them (i.e., made them change their character) down the pathway towards becoming a precursor of the human placenta. (ca.gov)
  • We have used a human endometrial epithelial cell line, to see if there are any effects, since we do not know if the levels of secreted products can indeed result in a response from the mother's uterus. (ca.gov)
  • The idea that cellular survival and growth could be maintained outside the body was recognized as possible almost hundred years ago when the German zoologist Wilhelm Roux described a successful experiment where he cultured chick neural crest in warm saline for a few days [ 1 ]. (intechopen.com)
  • Arguments supporting the reactive nature of LCH include the occurrence of spontaneous remissions, the extensive elaboration of multiple cytokines by dendritic cells and T-cells (the so-called cytokine storm) in LCH lesions, and the good survival rate in patients without organ dysfunction. (medscape.com)
  • FCDI offers several terminally differentiated cell types as catalog products: iCellĀ® Cardiomyocytes, iCell Neurons, iCell Endothelial Cells, and iCell Hepatocytes. (wikipedia.org)
  • Figure 5: Potential deleterious effects of senescent cells. (nature.com)
  • On the other hand, the infiltration of organs by a monoclonal population of aberrant cells, the possibility of lethal evolution, and the cancer-based modalities of successful treatment are all consistent with a neoplastic process. (medscape.com)
  • The translocation is acquired during a person's lifetime and is present only in abnormal blood cells. (medlineplus.gov)
  • The ultrastructural hallmark of the Langerhans cell, the Birbeck granule, was described a century later. (medscape.com)
  • This effect is due to the depth and density of the pigmented cells (or melanin granule dispersion) and the physical properties of light absorption and reflection described by the Tyndall light phenomenon or effect. (medscape.com)
  • When we proposed this study, we weren't sure whether it would even be possible to grow iPSCs from the blood of the participants in the Wellderly Study, since others have reported difficulty in making these stem cells from aged patients," Baldwin said. (sciencedaily.com)
  • Mechanosensitivity is common to a wide variety of cells in many different organisms ranging from bacteria to mammals. (springer.com)