• A new kind of medicine that destroys specific proteins on the surface of cells could lead to better therapies for conditions such as cancer. (newscientist.com)
  • As demonstrated on LF fibroblasts, the resistance to trypsin was similar in serum-supplemented and serum-free media, i.e., medium without cell adhesion-mediating proteins. (nature.com)
  • Based on all the results, the increased resistance to trypsinization of C2C12, LF, HaCaT, and VSMC cells on amine PPs can be explained most probably by a non-specific cell adhesion such as electrostatic interaction between the cells and amine groups on the material surface, rather than by the receptor-mediated adhesion through serum-derived proteins adsorbed on the PPs. (nature.com)
  • virtually all cell-surface and secreted proteins have complex sugars attached to them. (sciencedaily.com)
  • MemBrite™ Fix dyes covalently label cell surface proteins, and are available in with a wide selection of bright and photostable colors. (biotium.com)
  • There are specific receptor proteins like integrin on the external surface of focal attachments. (omicsonline.org)
  • And the internal surface has actin filaments such as talin, paxillin, vinculin, tensin and proteins that provide interactions between membrane receptor proteins [ 1 - 3 ]. (omicsonline.org)
  • Rising demand for biopharmaceutical products including antibodies, vaccines, proteins, and drugs is another major factor driving the growth of the cell culture protein surface coating market. (prnewswire.co.uk)
  • The production of biopharmaceutical products such as monoclonal antibodies and recombinant proteins is carried out by using cell culture technique. (prnewswire.co.uk)
  • Purified GST fusion proteins of the N and C termini of Na v 1.3 pull down contactin from lysates of transfected HEK 293 cells. (jneurosci.org)
  • This product category contains extracellular proteins of human, murine or rat sequence that, for the most part, initiate or otherwise affect signaling through cell surface receptors. (peprotech.com)
  • Cells interact with the external environment via a layer of proteins and respond to not only biochemical cues but also physical properties including stiffness and topography of adjacent surfaces. (gla.ac.uk)
  • Thus, users are advised to fully validate their application if they desire to obtain high quality data that includes surface proteins, intracellular proteins, and RNA expression data. (biolegend.com)
  • This environment-responsive phospholipid layer consisting of multiple dynamically balanced macromolecules, such as membrane proteins, carbohydrate and lipids, is regarded as a promising platform for various surface engineering strategies. (uky.edu)
  • We are currently developing these pipette-based techniques with the aim to use them to obtain fundamental knowledge about real biological problems such as how proteins interact and organize on the surface of cells and how different molecules interact locally with the cell membrane 6 . (lu.se)
  • CellBrite™ Fix dyes are fluorogenic membrane dyes that covalently stain the plasma membrane in live cells. (biotium.com)
  • We also offer classic membrane and membrane potential dyes, including CellBrite™ and CellBrite™ NIR dyes , which are lipophilic carbocyanine dyes that stain the plasma membrane of cells and are non-toxic for long-term tracking. (biotium.com)
  • It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine and stains yeast bud scars as well as the cell membrane of gram+ bacteria and mammalian cells. (biotium.com)
  • DiA 4-(4-Dihexadecylaminostyryl)-N-methylpyridinium iodide) is a green fluorescent membrane dye which diffuses much faster than DiO in cell membranes. (biotium.com)
  • 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine) is a widely used carbocyanine membrane dye that labels cell membranes by inserting its two long (C 18 carbon) hydrocarbon chains into the lipid bilayer. (biotium.com)
  • Important biological events associated with plasma membranes, such as signal transduction, cell adhesion, and protein trafficking, are mediated through the membrane microdomains. (mdpi.com)
  • Focal attachments have 10-15 nm distance between the cell membrane and the material surface and they are tight connections. (omicsonline.org)
  • The cell plasma membrane is an interactive interface playing an important role in regulating cell-to-cell, cell-to-tissue contact, and cell-to-environment responses. (uky.edu)
  • The crosslinking of a grafted polymeric patch from a mammalian cell membrane is achieved through surface-mediated photolithographic polymerization. (uky.edu)
  • Surface mounted cell phone lockers provide a convenient means of installation in locations where walls cannot accommodate a recessed mounted application. (lockers.com)
  • Human bmMSCs were described in the late nineties as well [ 6 ] and at the same time a breakthrough study investigated the expression of typical cell surface markers and the proliferation and differentiation properties of human MSCs in more detail [ 7 ]. (hindawi.com)
  • Cell attachment, proliferation and differentiation were studied using enzyme-linked immunosorbant assays, and immunofluorescence microscopy was used to detect integrin subunits and cytoskeletal arrangement 24 h after cellular attachment. (ucl.ac.uk)
  • The term "adhesion" to the biomaterial refers to the most important phase, since the quality of it will influence morphology and the capacity of cell proliferation and differentiation 3 . (bvsalud.org)
  • They display differentiation capacities and therefore qualify as multipotent progenitor cells (Figure 1 ). (hindawi.com)
  • Overview on self-renewal or differentiation of stem cells in their respective stem cell niche. (hindawi.com)
  • In this study, it was observed that the ion implantation stimulated the neural proliferation and the implantation of different ions on cell culture surfaces was essential to determine the effects of this technique on adhesion, proliferation, differentiation and apoptosis properties of cells in details. (omicsonline.org)
  • This was also the case for myogenic cell differentiation, which was indiscriminately higher on polymers with extended fibronectin, however, cytoskeletal contractility was found to play an essential role in the myogenic differentiation of cells on these polymers in a mobility dependent manner. (gla.ac.uk)
  • My observations suggest that fibronectin fibrils coating a polymer with low mobility may be most suited for osteogenic differentiation of hMSCs by simultaneously exposing cell-binding sites to a higher degree. (gla.ac.uk)
  • Biochemical assays used to measure cellular attachment and differentiation showed that there were no significant differences in the activity of cells grown on the different substrates. (ucl.ac.uk)
  • 2 (2007) observed that cell adhesion and proliferation, as well as the osteogenic differentiation of mouse mesenchymal stem cells (MSCs) to Ti discs were significantly similar to those on the plastic surface of the culture, indicating Ti as an excellent material for repairing hard tissue in the field of bone tissue engineering. (bvsalud.org)
  • Recent advances in live imaging and genetics of mammalian division, movement and cell differentiation leading to development which integrate observations of biochemical tissue formation [14 ]. (lu.se)
  • Various microfluidic approaches have been devised to mimic traversal in vitro by measuring the time required for cells to pass through a constriction. (nih.gov)
  • However, Neu5Gc expression on target cells did not enhance CPV or FPV infection in vitro. (nih.gov)
  • Expansion of MSCs was shown to be limited to a few passages of in vitro culture and the cells underwent replicative senescence [ 11 ]. (hindawi.com)
  • Therefore, available in vitro protocols for expansion of MSCs do not yield true stem cells. (hindawi.com)
  • The protein surface coating provides improved adhesion and proliferation of cells in vitro. (prnewswire.co.uk)
  • Activated cells can be prepared from in vivo -stimulated tissues or from in vitro -stimulated cultures (e.g. mitogen or LPS stimulation). (biolegend.com)
  • Short-term in vitro studies have shown that bone cells react more favourably to rough substrate surfaces than to smooth ones. (ucl.ac.uk)
  • This in vitro study investigated the effects of implant material composition and surface roughness on the early behaviour of three transformed human osteoblast-like cell lines, MG-63, HOS and U-2 OS, cultured on commercially pure titanium and zirconium substrates of different surface roughness. (ucl.ac.uk)
  • However, initial in vitro screenings mainly include the quantity of adherent immortalized cells and cytotoxicity. (bvsalud.org)
  • The approach, devised by Xin Zhou at the Dana-Farber Cancer Institute in Boston, Massachusetts, and her colleagues, involves using modified antibodies they call TransTACs to join a surface protein to another common protein that is involved in transporting iron into cells. (newscientist.com)
  • A bacterial fucosyltransferase can add antibodies to cell surfaces in a one-step reaction. (acs.org)
  • Antibodies and cell lines. (jneurosci.org)
  • The following protocol describes combined surface and intracellular protein staining with TotalSeq™-B antibodies, with or without TotalSeq™-B hashtag antibodies using the Single Cell 3' v3.1 Feature Barcoding technology from 10x Genomics. (biolegend.com)
  • This protocol has been validated solely for the detection of surface or intracellular protein expression with the corresponding TotalSeq™-B antibodies. (biolegend.com)
  • Optimization of antibody staining concentration is essential to obtain good quality data in antibody-based applications such as surface and intracellular staining using TotalSeq™ antibodies. (biolegend.com)
  • Enzymatic digestion may result in alterations of surface protein epitopes and impact staining with TotalSeq™ antibodies. (biolegend.com)
  • Chronic lymphocytic leukemia (CLL) and mantle cell lymphoma (MCL) are malignancies characterized by the dependence on B-cell receptor (BCR) signaling and by the high expression of ROR1, the cell surface receptor for Wnt-5a. (muni.cz)
  • [ 2 ] Options for second-line therapy in patients with relapsed/refractory disease include chemotherapy-free regimens with biologic targeted agents such as covalent Bruton tyrosine kinase (BTK) inhibitors, lenalidomide,venetoclax, and chimeric antigen receptor (CAR) T-cell therapy. (medscape.com)
  • Cell Type-Specific Adhesion and Migration on Laser-Structured Opaque Surfaces. (bvsalud.org)
  • Metastasis requires the penetration of cancer cells through tight spaces, which is mediated by the physical properties of the cells as well as their interactions with the confined environment. (nih.gov)
  • Cell-substrate interactions are of interest in modern biology. (gla.ac.uk)
  • It is not certain how cell-substrate interactions are affected by implant surface roughness, although integrins are thought to convey information about the substrate topography to the cells. (ucl.ac.uk)
  • A purely mechanistic theory of the cell-substrate interactions may not be sufficient to account for the variations in the reported behaviour of cells grown on different substrates. (ucl.ac.uk)
  • In my presentation, I will show how model membranes of varying complexity can be used in complement to virological investigations to study how viruses interact at the cell surface and how such interactions are optimized, on a molecular level, to promote efficient viral entry and infection. (lu.se)
  • Furthermore, we examined the three-dimensional cell interactions with spike topographies and developed a tailored migration assay for long-term monitoring on opaque materials. (bvsalud.org)
  • Many of the most important interactions in the human body take place between molecules on the surface of cells. (lu.se)
  • interactions between cells. (lu.se)
  • Our previously-obtained impressive results of highly increased C2C12 mouse myoblast adhesion to amine plasma polymers (PPs) motivated current detailed studies of cell resistance to trypsinization, cell proliferation, motility, and the rate of attachment carried out for fibroblasts (LF), keratinocytes (HaCaT), rat vascular smooth muscle cells (VSMC), and endothelial cells (HUVEC, HSVEC, and CPAE) on three different amine PPs. (nature.com)
  • Here we report the modification of the chemical and mechanical properties of ultra-high molecular weight poly(ethylene) (UHMWPE) using 80:20 (v/v) N 2 /H 2 ASPN, followed by growth of 3T3 fibroblasts on the treated and untreated polymer surfaces. (open.ac.uk)
  • The MSCs have been described for the first time as colony forming fibroblasts (CFU-F), a rare population of cells residing in the bone marrow of guinea-pigs or mice [ 1 , 2 ]. (hindawi.com)
  • Fibronectin binding sites on cultured human fibroblasts were localized by high voltage electron microscopy using either 5- or 18-nm colloidal gold beads (Au5 or Au18) bound to intact fibronectin, the 70-kD amino-terminal fragment of fibronectin that blocks incorporation of exogenous fibronectin into extracellular matrix, or 160-180-kD fragments of fibronectin with cell adhesion and heparin-binding activities. (rupress.org)
  • We investigated different laser -fabricated spike structures using primary and immortalized cell lines of fibroblasts and osteoblasts and included quantification of the cell area, aspect ratio, and focal adhesions . (bvsalud.org)
  • While fibroblasts and osteoblasts on small spikes retained their normal morphology, cells on medium and large spikes sank into the structures, affecting the composition of the cytoskeleton and thereby changing cell shape . (bvsalud.org)
  • Thus, developing a non-invasive and touch-free detection of cell growth in longitudinal studies of 3D tumor spheroid cultures or of stem cell regeneration remains a major unmet need. (unboundmedicine.com)
  • Further, SH-SAW biosensing was applied to A549 cells cultured on a 3D electrospun nanofiber scaffold that generate tumor spheroids (tumoroids) and the results showed the device's ability to detect changes in tumor spheroid growth over the course of eight days. (unboundmedicine.com)
  • As test cases to examine changes in protein presence associated with tumor growth inhibition, we applied SILAC-based proteomics on human colon carcinoma cells treated with galectin-4 (Gal-4). (uni-muenchen.de)
  • Mantle cell lymphoma (MCL) is a lymphoproliferative disorder derived from a subset of naive pregerminal center cells localized in primary follicles or in the mantle region of secondary follicles. (medscape.com)
  • Mantle cell lymphoma (MCL) is recognized in the Revised European-American Lymphoma and World Health Organization classifications as a distinct clinicopathologic entity. (medscape.com)
  • Cell Surface Profiling Using High-Throughput Flow Cytometry: A Platform for Biomarker Discovery and Analysis of Cellular Heterogeneity. (stembook.org)
  • 4F2 cell-surface antigen heavy chain is a protein that in humans is encoded by the SLC3A2 (solute carrier family 3 member 2) gene. (wikipedia.org)
  • DURHAM, N.C. - Researchers at the Duke Cancer Institute who have been studying prostate cancer cells for decades now think they know why PSA (prostate-specific antigen) levels reflect cancer progression. (scienceblog.com)
  • We correlate the detachment force of antigen-interacted MSCs by a parallel laminar flow assay with the density of ICAM on the substrate and the density of anti-ICAM on the MSC surface. (uky.edu)
  • This study was done to evaluate efficacy of HBV vaccination on hepatitis B virus surface antigen (HBsAg) carrier rate in children with thalassaemia major receiving multiple blood transfusions. (who.int)
  • 2 We have also delivered molecules from nm-sized pipettes to the surface of neuronal cells to locally stimulate and study heat- and pain-sensitive receptors, 3 and delivered small molecular drugs to study receptors on heart cells 4,5 . (lu.se)
  • Taken together, these results demonstrate the use of SH-SAW device for detection and quantification of cell growth changes over time in 2D suspension cultures and in 3D cell culture models, which may have potential applications in both longitudinal 3D cell cultures in cancer biology and in regenerative medicine. (unboundmedicine.com)
  • Therefore, many laboratories try with great effort to develop resorbable tissue scaffolds that could support the patient´s cells. (nature.com)
  • ECM provides mechanical support for cells and also determines the shape of tissue 9 . (nature.com)
  • In contrast to standard tissue culture surfaces which are optimised to enhance conditions for cell attachment, the cell-repellent surface has been developed to effectively prevent cell attachment.CELLSTAR® cell culture vessels with a cell-repellent surface reliably prevent cell attachment in suspension cultures of semiadherent and adherent cell lines where standard hydrophobic surfaces generally used for suspension culture are insufficient. (thomassci.com)
  • Tissue stem cells live in their organ niches where they represent a minute cell population. (ca.gov)
  • Traditional cell-screening techniques such as FACS and MACS are better suited for large numbers of cells isolated from bulk tissue and cannot easily screen stem or progenitor cells from minute populations found in their physiological niches. (ca.gov)
  • However, even though polymeric biomaterials have been described as one of the cornerstones of tissue engineering, the effect of an intrinsic polymer property known as mobility on cell behaviour is poorly characterised. (gla.ac.uk)
  • BioLegend has not tested this protocol using single-cell suspensions derived from enzymatically digested tissue. (biolegend.com)
  • Surface associated glycoproteins of endothelial origin, or derived from pericytes, intravascular leukocytes, and plasma, are other important components of the glycocalyx, constituting a vascular cell surface proteome that is dynamic, tissue-specific, and sensitive to changes in vascular homeostasis, blood infection, and inflammation. (lu.se)
  • In the second part of this dissertation, we study the use of tissue-adhesive coatings to improve the retention of therapeutic mesenchymal stem cells (MSCs) in the heart following intramyocardial or intravenous injection. (uky.edu)
  • The foregoing properties allow the scarring and maintenance of tissue structure adhesion to the Ti surface 1 . (bvsalud.org)
  • Other vital parameters , such as cell migration and an in-depth understanding of the interaction between native tissue cells and implant surfaces, are rarely considered. (bvsalud.org)
  • of the most critical events which lead to tissue patterning involves mechanisms going beyond single cells. (lu.se)
  • These oxides form during the cutting, cleaning, and implant sterilization process and remain adherent to the surface. (bvsalud.org)
  • Cell phone storage lockers can be ordered with resettable combination locks or master keyed locks. (lockers.com)
  • It was therefore implied that the difference in cell behaviour observed on the substrates of different height might be a result of an interplay between the lateral mechanics and the rate of liquid flow though the gel. (gla.ac.uk)
  • Plasma technology for surface modifications has many advantages compared to morphological and chemical treatments, such as low toxicity, short time of production, substrate independence and, if required, a negligible degradation of the original material. (nature.com)
  • Working in the MIT.nano clean room, they coat the solar cell structure using a slot-die coater, which deposits layers of the electronic materials onto a prepared, releasable substrate that is only 3 microns thick. (eurekalert.org)
  • To solve this challenge, the MIT team searched for a lightweight, flexible, and high-strength substrate they could adhere the solar cells to. (eurekalert.org)
  • The mechanical anisotropy of the gel due to its being bound to the substrate was hypothesised to be the cause of the cell behaviour observed. (gla.ac.uk)
  • I verified using atomic force microscopy the similarity in topography and stiffness between the four substrate surfaces and ascertained that fibronectin molecules adsorb in a globular conformation on the polymer with the shortest side chain (1 methyl group) compared with a more extended conformation on the rest of the polymers. (gla.ac.uk)
  • My study of the fibronectin coatings using fluorescence recovery after photobleaching (FRAP) on the different polymer surfaces suggested that the mobility of the polymer substrate is translated to the interfacial protein layer. (gla.ac.uk)
  • RNA Crossing Membranes: Systems and Mechanisms Contextualizing Extracellular RNA and Cell Surface GlycoRNAs. (stembook.org)
  • Combined with advanced fluorescence microscopy (single molecule, FRAP, TIRF) makes this a powerful method to study the properties and function of different molecules in cell membranes. (lu.se)
  • Boston) - Researchers from the Boston University School of Medicine (BUSM) have demonstrated that commensal species of the genus Neisseriae are low inducers of human airway epithelial cell responses as compared to the pathogenic species. (scienceblog.com)
  • However, transplanting other than hematopoietic stem and progenitor cells is still limited to a few applications, and it mainly applies to mesenchymal stromal cells (MSCs) isolated from bone marrow. (hindawi.com)
  • At the same time, by asymmetric cell division or after specific activation, stem cells must be able to generate more mature progenitor cells or differentiated effector cells (Figure 1 ). (hindawi.com)
  • This method, as well as the cell attachment rate, proved the difference among the plasma polymers with different amounts of amine groups, but other investigated techniques could not reveal the differences in the cell behaviour on different amine PPs. (nature.com)
  • However, the effect gel geometry (thickness, width and length) has on both the mechanics of the gel and the cells behaviour has usually been ignored. (gla.ac.uk)
  • Therefore, films of four poly(alkyl acrylates) with similar surface chemistry but different glass transition temperatures achieved by varying branch chain lengths (1, 2, 4 or 6 methyl groups) were used in this work to investigate the role of polymer mobility on cell behaviour. (gla.ac.uk)
  • Fibroblast coated surfaces were maintained for up to 28 days, monitoring their metabolic activity and morphology throughout. (open.ac.uk)
  • Results: The proliferation of endothelial cells on the titanium surface is better than the culture plate surfaces, the adhesion cells on titanium surface are more numbers, cell morphology is more extended, titanium surface expression of VE-cadherin protein higher than the culture plate surface. (atlantis-press.com)
  • Aim: To evaluate the adhesion of mouse bone marrow mesenchymal cells (MBMMC) on different titanium surfaces. (bvsalud.org)
  • mesenchymal stem cells, bone marrow, titanium. (bvsalud.org)
  • We suggest that such dimers mayhave relevance as mediators of signal transduction in T cells. (rcsb.org)
  • We demonstrated the striking difference in the resistance to trypsin treatment between endothelial and non-endothelial cells. (nature.com)
  • The increased resistance observed for the non-endothelial cell types was accompanied by an increased rate of cellular attachment, even though spontaneous migration was comparable to the control, i.e., to the standard cultivation surface. (nature.com)
  • To observe the proliferation and adhesion of umbilical cord endothelial cells (HUVEC) on the titanium (Ti) surface with large particles blasted-etched (SLA) treatments and further clarify the mechanism of action of each other between the Ti implant surface and the body. (atlantis-press.com)
  • Methods: The human umbilical cord endothelial cells are cultured on the surface of SLA-Ti disks and culture plates. (atlantis-press.com)
  • The proliferation of endothelial cells is detected by MTT method and adhesion of endothelial cells observed by PI fluorescence staining and western-bolt detection of protein of VE-cadherin. (atlantis-press.com)
  • Conclusion: The SLA Ti surface can promote the proliferation and adhesion of endothelial cell and could promote the early healing process of osseointegration. (atlantis-press.com)
  • Contactin/F3, a cell adhesion molecule, has been shown to interact with and enhance surface expression of sodium channels Na v 1.2 and Na v 1.9. (jneurosci.org)
  • and stem cell regeneration. (lu.se)
  • With longer incubations, Au18-fibronectin complexes were arranged into long fibrillar networks on the cell surface and in the extracellular space. (rupress.org)
  • In this context, we employ single particle tracking together with atomic-force-microscopy-based force spectroscopy to study how virus attachment, detachment and diffusion is modulated at the cell surface. (lu.se)
  • This was supported with another set of cell experiments on such samples, where the cell did not respond in accordance with the stiffness as measured by AFM. (gla.ac.uk)
  • This interesting finding highlighted a possible pathway for cells cultured on fibronectin coated polymer surfaces to detect the underlying polymer mobility via the fibronectin coating. (gla.ac.uk)
  • Carbon-based electrodes in polymer electrolyte fuel cells (PEFCs) are prone to corrosion. (strath.ac.uk)
  • We therefore briefly discuss differences found in subsets of human bmMSCs and in MSCs isolated from some other sources and touch upon how this could be utilized for cell-based therapies. (hindawi.com)
  • Overview of studies published regarding MSCs using the term "stem cell" or "stromal cell" in the last 20 years accessed by a web search in July 2015 (Google Scholar). (hindawi.com)
  • It seems that the term "stem cell" became more popular although the "stemness" was only shown in a more strict sense for MSCs involved in osteogenesis and bone repair. (hindawi.com)
  • When the biological properties of MSCs were explored in more detail, questions arose whether these cells met the criterion of a true stem cell [ 8 ]. (hindawi.com)
  • MSCs were also investigated for stem cell qualities in vivo. (hindawi.com)
  • Moreover, a dramatic reduction of immune cell quantity was observed after intravenous injection, indicating the enhanced immunoregulatory efficacy by systemically delivering ICAM-adhesive MSCs to the site of inflammation. (uky.edu)
  • Therefore, the present study aimed to evaluate the adhesion capacity of mouse bone marrow MSCs to smooth and plasmanitrided Ti surfaces in the cathodic cage configuration. (bvsalud.org)
  • image: MIT researchers have developed a scalable fabrication technique to produce ultrathin, lightweight solar cells that can be stuck onto any surface. (eurekalert.org)
  • MIT engineers have developed ultralight fabric solar cells that can quickly and easily turn any surface into a power source. (eurekalert.org)
  • These durable, flexible solar cells, which are much thinner than a human hair, are glued to a strong, lightweight fabric, making them easy to install on a fixed surface. (eurekalert.org)
  • Traditional silicon solar cells are fragile, so they must be encased in glass and packaged in heavy, thick aluminum framing, which limits where and how they can be deployed. (eurekalert.org)
  • Six years ago, the ONE Lab team produced solar cells using an emerging class of thin-film materials that were so lightweight they could sit on top of a soap bubble . (eurekalert.org)
  • But these ultrathin solar cells were fabricated using complex, vacuum-based processes, which can be expensive and challenging to scale up. (eurekalert.org)
  • In this work, they set out to develop thin-film solar cells that are entirely printable, using ink-based materials and scalable fabrication techniques. (eurekalert.org)
  • To produce the solar cells, they use nanomaterials that are in the form of a printable electronic inks. (eurekalert.org)
  • While it might appear simpler to just print the solar cells directly on the fabric, this would limit the selection of possible fabrics or other receiving surfaces to the ones that are chemically and thermally compatible with all the processing steps needed to make the devices. (eurekalert.org)
  • InSight Deploys its Solar Cells, Prepared for Surface Operations on Mars! (universetoday.com)
  • The expression of contactin and Na v 1.3 in the HEK-Na v 1.3 stable cell line significantly increases the current density. (jneurosci.org)
  • Furthermore, these techniques rely upon irreversible antibody binding, potentially altering cell properties, including gene expression and regenerative capacity. (ca.gov)
  • To address these challenges, we have developed a novel, label-free stem-cell analysis and sorting platform capable of quantifying cell-surface marker expression of single functional organ stem cells directly isolated from their micro-anatomical niche. (ca.gov)
  • Immunofluorescence studies detected no marked variation in the integrin subunit expression and organisation of the cytoskeletal network of the cells. (ucl.ac.uk)
  • By tracing significant alterations of protein expression likely relevant for the observed phenotypic effects, the capacity of a galectin to affect the proteome of human colon cancer cells at multiple sites is revealed. (uni-muenchen.de)
  • Quantity of envelope on cell surfaces and gp120 on virions. (figshare.com)
  • The elastic modulus, surface topography, and adhesion properties of the ASPN-treated UHMWPE surface were studied over 28 days during sample storage under ambient conditions and during immersion in two commonly used cell culture media. (open.ac.uk)
  • The experiments indicate that the cell lines may not discriminate between the surface chemistry and topography of the metals used in the study. (ucl.ac.uk)
  • I want to get the latest chemistry news from C&EN in my inbox every week. (acs.org)
  • Single cell experiments [ 11 ] could provide iterative validation of models with experimental results. (lu.se)
  • Here, we describe an experimental protocol to chemically tag and quantify the vascular cell surface proteome in murine models of bacteremia, in a time-resolved and. (lu.se)
  • Localization of cell surface sites involved in fibronectin fibrillogenesis. (rupress.org)
  • Sorting single satellite cells from individual myofibers reveals heterogeneity in cell-surface markers and myogenic capacity. (ca.gov)
  • It was discovered that a cell differentially spreads and preferentially accumulates at a certain position with respect to the local variation in thickness along a wedge gel (thickness varied from ~50 to 400 µm). (gla.ac.uk)
  • The simulated relative frequency shift from the two oscillatory circuit systems (one of which functions as control) were found to be concordant to experimental data generated with RAW264.7 macrophage and A549 cancer cells. (unboundmedicine.com)
  • We then optimized the patterning approach for the formation of PEGDA patches on live A549 cells. (uky.edu)
  • Surface roughness of the substrates was quantified using two techniques: a contact profilometer, and a non-contacting laser scanning profilometer. (ucl.ac.uk)
  • that is, they increase resistance to corrosion and surface roughness 6 . (bvsalud.org)
  • When germ-free cell cultures became a laboratory routine, hopes were high for using this novel technology for treatment of diseases or replacement of cells in patients suffering from injury, inflammation, or cancer or even refreshing cells in the elderly. (hindawi.com)
  • Detection and quantification of cell viability and growth in two-dimensional (2D) and three-dimensional (3D) cell cultures commonly involve harvesting of cells and therefore requires a parallel set-up of several replicates for time-lapse or dose-response studies. (unboundmedicine.com)
  • The use of live cells as therapies could become more widespread if there were easy ways to modify cell surfaces. (acs.org)
  • Changing surface characteristics of scaffolds was a way to promote regeneration and ion implantation was one of the methods to modify surface properties which play a huge role in enhancing the proliferation and integration of cells. (omicsonline.org)
  • Today, more than 50 years after the first successful bone marrow transplantation, clinical application of hematopoietic stem cells is a routine procedure, saving the lives of many every day. (hindawi.com)
  • Adhesion of cells to implant surfaces is a major factor in determining the pattern of events in osseointegration. (ucl.ac.uk)
  • The physicochemical properties of Ti implant surfaces are fundamental to the success of osseointegration. (bvsalud.org)
  • To improve the biological responses for obtaining rapid osseointegration, the surfaces have been modified by a wide range of process involving mechanical, chemical, and physical surface treatment methods, such as: the Ti plasma spray treatment, Ti oxide blasting, laser deposition of Ti carbide, and acid conditioning 4 . (bvsalud.org)
  • Trastuzumab-NK conjugates are more effective than unmodified NK cells in killing cancer cells both in cell culture and in mice. (acs.org)
  • The system of surface bound hydrogels is commonly used as a cell culture surface in the field of cell biomechanics. (gla.ac.uk)
  • Research and Markets has announced the addition of the "Cell Culture Protein Surface Coating Market Analysis By Coating Type (Self-Coating, Precoating [Multiwall/Microwell Plates, Petri Dishes, Flasks, Slides, Cover Slips]), Protein Source (Plant, Animal, Human, Synthetic) And Segment Forecast 2014-2025" report to their offering. (prnewswire.co.uk)
  • Thus, the demand of cell culture protein surface coating is growing at a high rate, which is estimated to drive the growth of this sector. (prnewswire.co.uk)
  • Self-coating was the largest revenue grossing segment of the cell culture protein surface coatingmarket in 2015. (prnewswire.co.uk)
  • Herein, we demonstrate that a shear horizontal-surface acoustic waves (SH-SAW) device comprising two pairs of resonators consisting of interdigital transducers and reflecting fingers can be used to quantify mass loading by the cells in suspension as well as within a 3D cell culture platform. (unboundmedicine.com)
  • MBMMC were cultured on titanium discs in 24-well cell culture plates, at a density of 1 x 104 cells per well. (bvsalud.org)
  • The researchers demonstrated the method by attaching trastuzumab, an antibody used for cancer treatment, to the surface of natural killer (NK) immune cells. (acs.org)
  • My ELISA analyses indicated a higher exposure of these important cell-binding sites on the more extended fibronectin compared with the globular one however, this did not correlate to the mobility of polymers or the mobility of the fibronectin layer. (gla.ac.uk)
  • Ici, on adapte les essais de cytométrie en flux pour fournir une quantification de l'expression de la protéine totale et de la surface cellulaire relative des canaux ioniques recombinants exprimés dans des cellules 201 TSA. (jove.com)
  • Defects on cell-surface sugars may promote the short-term inflammation and long-term neurodegeneration that occurs in the central nervous system of multiple sclerosis patients, according to a new article. (sciencedaily.com)
  • Ti is a very stable metal, although slight oxide formation occurs on its surface. (bvsalud.org)
  • This results in the formation of oxidized titanium nanostructures (such as nanosheets and nanotubes) at the surface. (strath.ac.uk)
  • Conclusions: It was demonstrated that titanium surface treatment with ionic nitriding in a cathodic cage is biocompatible since it preserved the integrity of the cultivated MBMMC for a period of 24 h, allowing their adhesion. (bvsalud.org)
  • Results reveal that after optimizing the local surface nanocrystallization layout, the specific energy absorption (SEA) is increased by 50.78% compared with the untreated counterpart. (techscience.com)
  • Binding sites for Au18-fibronectin on the cell surface were localized to specific regions along the edge of the fibroblast and on retraction fibers. (rupress.org)
  • The appearance of Au18-fibronectin in these fibrillar networks and disappearance of clusters of Au18-fibronectin suggest that Au18-fibronectin complexes are arranged into matrix at specific regions of the cell surface. (rupress.org)
  • Select specific criteria to produce cross-tabulated average land surface temperatures measures for day or night. (cdc.gov)
  • Ident- cult to deduce interaction rules from observations of the ified transcription factors for specific cell fates are, for entire system. (lu.se)