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  • tumor
  • The invention provides a method for preparing cell populations with anti-tumor immune response activity, which includes co-culturing tumor and mononuclear cell in a three-dimensional cell culture device, separating and amplifying the cell populations with anti-tumor immune response activity from the cultures. (patentgenius.com)
  • The present invention, at the same time, discloses the cell populations with anti-tumor immune response activity obtained by the method and the kit comprising the cell populations. (patentgenius.com)
  • and wherein thetumor cells and mononuclear cells are put together in the one or more empty cavities of the three-dimensional cell culture unit within the cell culture medium thereby forming a co-culture of tumor cells and mononuclear cells resulting in a cellpopulation with anti-tumor immune response activity. (patentgenius.com)
  • 3. The method of claim 1, wherein the co-culture of tumor cells and mononuclear cells has a ratio of mononuclear cells to tumor cells which is in the range of 5:1 to 100:1. (patentgenius.com)
  • 6. A cell population with anti-tumor immune response activity, prepared by the method of claim 1 wherein the number of cells in the population is from 10.sup.5 to 10.sup.11. (patentgenius.com)
  • 8. A method for the treatment of a patient having a tumor, wherein the method includes the step of injecting an infusion solution that contains the cell population with anti-tumor immune response activity prepared by the method of claim 1 intoa cancer patient in need of the treatment, wherein the mononuclear cells are derived from the patient. (patentgenius.com)
  • 9. A pharmaceutical composition for active specific immunotherapy for administration to a patient in need of the treatment, comprising using the cell population with anti-tumor immune response activity obtained by the method of claim 1, whereinthe mononuclear cells in the co-culture are derived from the patient. (patentgenius.com)
  • Sallusto F, Lanzavecchia A. Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor alpha. (pubmedcentralcanada.ca)
  • Cytokine-induced killer (CIK)cells are shown to be a heterogeneous population, and the major population expresses both the T cell marker CD3 and the NK cell marker CD56, and is termed NKT cells, which has shown significant anti-tumor activity in both clinical trials and animal studies. (clinicaltrials.gov)
  • Furthermore, CIK cells are able to expand significantly when they are cultured with DCs, and the CIK cells activated by DCs stimulation (DCIKs)have a characteristic which cytotoxic activity enhanced and show increased anti-tumor activity. (clinicaltrials.gov)
  • This study aimed to evaluate the clinical efficacy of DCIK cells treatment combined with DCs following tumor resection and radiotherapy in patients with malignant glioma. (clinicaltrials.gov)
  • Dendritic cells pulsed With tumor lysate were injected back into the patient intradermally close to a lymph node, DC vaccinations will be given every week for a total of four vaccinations. (clinicaltrials.gov)
  • In our review, we discuss the various subtypes of tumor antigens with a focus on neoantigens and their potential in cancer immunotherapy. (frontiersin.org)
  • We will describe the current methods and techniques of detection as well as the structural requirements for neoantigens that are needed for their recognition by T cells and for tumor destruction. (frontiersin.org)
  • Immunology
  • He is conducting studies looking into factors that influence HPV-related cancer development and skin cancer development using molecular cell biology, immunology and next-generation sequencing approaches. (edu.au)
  • Immense Immunology Insight: Question: Which cells have which MHC class? (blogspot.com)
  • epitopes
  • It ensures that a single antigen is recognized and attacked through its overlapping parts, called epitopes , by multiple clones of B cell . (wikipedia.org)
  • Furthermore, we optimized four B cell epitopes to design a novel DKK1 multiepitope DNA vaccine and evaluated its bone protective effects in collagen-induced arthritis (CIA), a mouse model of RA. (hindawi.com)
  • mice
  • To investigate their role in B cell development, we generated B cell-specific ADAM10 knockout mice. (rupress.org)
  • Leslie Brent, Rupert Billingham and technician Trevor Courtenay at University College London in 1955/1956, working out the intravenous route for the inoculation of cells into newborn mice. (royalsocietypublishing.org)
  • Neural stem cells can repair damaged parts of the brain and restore motor impairments in mice that display features of cerebral palsy, according to new research published in eNeuro. (medicalxpress.com)
  • innate
  • 0.01) and an increase in GILT mRNA expression, indicating increased innate immune responses by these cells in treated animals. (aacrjournals.org)
  • Bacterial infections As described earlier, phagocytic innate insusceptible cells are ticklish in regard to the ini- tial immune identification of bacteria result of PAMPs such as lipopolysaccharide and lipoteichoic acids in the bacterial room wall. (autoportal.ru)
  • cytokine
  • 4. The method of claim 1, further comprising adding a substance that can promote the growth of mononuclear cells into the liquid cell culture medium in the three-dimensional cell culture device in step (1), wherein the substance is a cytokine. (patentgenius.com)
  • These cells upon receiving a cytokine signal trigger differentiation of effector Tcell populations. (frontiersin.org)
  • therapeutic
  • This study presents the proof of concept of a previously unidentified real-time and single-cell approach to investigate cellular biology, signal messengers, and therapeutic effects with nanomaterials. (jove.com)
  • deletion
  • Intriguingly, deletion of ADAM10 prevented development of the entire marginal zone B cell (MZB) lineage. (rupress.org)
  • specifically, effective vaccine-induced T cells could become exhausted through the loss of replicative capacity and apoptotic deletion. (iayork.com)
  • extracellular
  • Although 38 ADAMs have been identified, ADAM10 has emerged as one of several members that regulates cell processes by cleaving or shedding extracellular domains of numerous transmembrane receptors and ligands. (rupress.org)
  • cancer
  • MSC are unique bone marrow-derived multipotent progenitor cells that are presently being exploited as gene therapy vectors for a variety of conditions, including cancer and autoimmune diseases. (frontiersin.org)
  • transplant
  • Transplant rejection originally considered as cell mediated response, now has shown to involve humoral response and complement system. (frontiersin.org)
  • human
  • There are at least 4 main classes of antigen presenting cells present in mouse and human skin. (edu.au)
  • Principles for adoptive T cell therapy of human viral diseases. (pubmedcentralcanada.ca)
  • Human leukocyte antigen (HLA) haplotypes DR3-DQ2.5, DR5-DQ7/DR7-DQ2.2, and DR4-DQ8 are the main genetic risk factors associated with CD and the absence of their respective alleles practically excluded the condition. (hindawi.com)
  • A tissue-like structure created from human stem cells and implanted into a damaged region of the mouse brain improves cell survival and differentiation relative to conventional, cell-based methods. (medicalxpress.com)
  • conventional
  • However, conventional classification approaches can be ill-describing the effects and i'm interested in using state-of-the-art single-cell technology to characterize this interesting type of cells. (edu.au)
  • Also, conventional laboratory extractions mostly use bulky materials that ignore the heterogeneity of each cell. (jove.com)
  • Conventional chemotherapies target tumors but also dividing cells in healthy organs and are therefore frequently associated with significant toxicity. (frontiersin.org)
  • RBCs
  • Burnet's hypothesis was influenced by a report from Ray Owen ( figure 3 ) that, as adults, twin cattle had circulating red blood cells (RBCs) bearing genetic markers of both twins [ 9 ]. (royalsocietypublishing.org)
  • Owing to a shared placental circulation they became chimaeras with respect to erythropoietic stem cells (the half-life of RBCs is short, requiring constant replenishment from stem cells). (royalsocietypublishing.org)
  • MHC class I is present on all nucleated cells except RBCs. (blogspot.com)
  • regulates
  • The proteolytic activity of a disintegrin and metalloproteinase 10 (ADAM10) regulates cell-fate decisions in Drosophila and mouse embryos. (rupress.org)
  • vaccines
  • Vaccines may contain living, weakened (attenuated) organisms (measles), killed whole organisms (whole cell pertussis, influenza), portions of organisms (subunit influenza), purified components of organisms (acellular pertussis, pneumococcal polysaccharide), or they may be manufactured artificially (hepatitis B produced by recombinant DNA technology). (encyclopedia.com)
  • For some diseases, vaccines may be available in more than one form (live attenuated and inactivated [killed] poliovirus vaccines, whole cell and acellular pertussis vaccines). (encyclopedia.com)
  • rejection
  • Activated T-cells are the predominant mediators of immunologic rejection. (blogspot.com)
  • BACKGROUND: The copy number of donor-derived cell-free DNA (dd-cfDNA) in blood correlates with acute rejection (AR) in heart transplantation. (stanford.edu)
  • heterogeneity
  • The aim is to discover whether we can target molecular switches in individual cells and sufficiently alter the immune cellular heterogeneity within this environment to favour the clearance of HPV, so that we can consistently prevent the progression of the disease when HPV is involved. (edu.au)
  • activation
  • This production is tightly regulated and requires the activation of B cells by activated T cells (another type of white blood cell), which is a sequential procedure. (wikipedia.org)
  • In diseases that demonstrate IC formation and complement activation also show hyperactive CD4+ T-cell response. (frontiersin.org)
  • organs
  • The passenger leukocyte theory suggests that graft leukocytes that express donor major histocompatibility complexes (MHCs) migrate to recipient lymphoid organs, where they activate host T cells. (sciencemag.org)
  • level
  • High level expression of the designed vaccine was measured in supernatant of COS7 cells. (hindawi.com)
  • Using a testing model with overexpression of GFP, the nanotubes successfully transported the intracellular GFP out at the single-cell level. (jove.com)
  • potential
  • Positive ions already present in the axon are repelled, and pushed towards the next node, at which point the action potential is regenerated. (davidson.edu)
  • vaccine
  • We hypothesize that the unique properties of modified MSC may enable these cells to serve as an unconventional but innovative, vaccine platform (described in Figure 1A ). (frontiersin.org)