• Kaempferol has been shown to inhibit cell growth, induce apoptosis and cell cycle arrest in several tumors, but not in renal cell carcinoma (RCC). (nih.gov)
  • MTT assay and colony formation assay were used to study cell growth, and flow cytometry was used to study apoptosis and cell cycles in different RCC cells treated with various doses of kaempferol. (nih.gov)
  • A significant inhibition on cell growth, induction of apoptosis and cell cycle arrest were observed in 786-O and 769-P cells after kaempferol treatment compared with the control group. (nih.gov)
  • Apoptosis was measured by Hoechst 33258/propidium iodide double staining of nuclear chromatin and the formation of gaps into the lymphendothelial barrier in a three-dimensional co-culture model consisting of MCF-7 tumour cell spheroids and human lymphendothelial monolayers. (nature.com)
  • In HL-60 leukaemia cells, di-GA activated caspase 3 and dose-dependently induced apoptosis. (nature.com)
  • These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)
  • The aim of the present investigation was to elucidate the cellular mechanisms whereby Tanshinone IIA (Tan IIA) leads to cell cycle arrest and apoptosis in vitro in keratinocytes, the target cells in psoriasis. (hindawi.com)
  • Tan IIA inhibited proliferation of mouse keratinocytes in a dose- and time-dependent manner and induced apoptosis, resulting in S phase arrest accompanied by down-regulation of pCdk2 and cyclin A protein expression. (hindawi.com)
  • Furthermore, Tan IIA-induced apoptosis and mitochondrial membrane potential changes were also further demonstrated by DNA fragmentation, single-cell gel electrophoresis assay (SCGE), and flow cytometry methods. (hindawi.com)
  • Therefore, based on clinical experience as well as cytotoxic activity against multiple human cancer cells, we hypothesized that induction of apoptosis is the underlying mechanism for the treatment of psoriasis. (hindawi.com)
  • Expression of cell cycle inhibitors, P21 and P27, and activation of apoptosis executer enzyme, caspase-3, were also induced by TCEE. (hindawi.com)
  • This TCEE also demonstrates antitumor properties such as the induction of cell cycle arrest and activation of apoptosis on human colon, lung, melanoma, osteosarcoma, and pancreatic cancer cells [ 16 - 19 ]. (hindawi.com)
  • Resveratrol could play a toxic role through inducing apoptosis of the cancer cell in a time- and concentration-dependent manner. (mdpi.com)
  • The inhibition of cell proliferation was related to arrest of the cell cycle in the G0/G1 phase and increased apoptosis in response to PTGR1 knockdown as indicated by flow cytometry. (go.jp)
  • In addition, cell cycle distribution analysis revealed that G0/G1 phase arrest was induced following pristimerin treatment in CAL‑27 and SCC‑25 cells, which was strongly associated with upregulation of p21 and p27, coupled with downregulation of cyclin D1 and cyclin E. Meanwhile, pristimerin induced significant apoptosis of CAL‑27 and SCC‑25 cells, alongside decreased levels of caspase‑3 and specific cleavage of poly (ADP‑ribose) polymerase. (spandidos-publications.com)
  • Pristimerin has also been reported to induce apoptosis of various human cancer cells, including in multiple myeloma ( 10 ), breast ( 11 ), liver ( 12 ), pancreatic ( 13 ) and prostate cancer ( 14 ). (spandidos-publications.com)
  • Pristimerin exhibited potent anti-proliferative and apoptosis-inducing effects on the OSCC cell lines CAL-27 and SCC-25. (spandidos-publications.com)
  • We show here that expression of EBF3 resulted in cell cycle arrest and apoptosis. (aacrjournals.org)
  • Cycloheximide or Chk2 inhibitor pretreatment abrogated not only activation of Chk2 but also G 2 /M arrest and apoptosis mediated by DIM. (aspetjournals.org)
  • Blocking ROS generation by N -acetyl cysteine protects the cells from DIM-mediated G 2 /M arrest and apoptosis. (aspetjournals.org)
  • Hoechst 33258 staining was performed to determine if VALD-3 induced apoptosis of MCF-7 and MDA-MB-231 cells and Annexin V/PI staining was performed to quantify the percentages of apoptosis. (researchsquare.com)
  • Flow cytometry analysis showed that VALD-3 triggered cell cycle arrest and induced apoptosis of breast cancer cells. (researchsquare.com)
  • This study is the first to show that VALD-3 induces apoptosis and cell cycle arrest in human breast cancer cells by suppressing Wnt/β-catenin signaling, indicating that it could be a potential drug for the treatment of breast cancer. (researchsquare.com)
  • Cell cycle analysis suggested activation of apoptosis, without arresting cell cycle progression. (nature.com)
  • Annexin V-propidium iodide double-staining demonstrated that Sapota fruit extracts potentiate apoptosis rather than necrosis in cancer cells. (nature.com)
  • Scholars@Duke publication: Inhibition of Isoprenylcysteine Carboxylmethyltransferase Induces Cell-Cycle Arrest and Apoptosis through p21 and p21-Regulated BNIP3 Induction in Pancreatic Cancer. (duke.edu)
  • Thus, unlike other tumor suppressors, RPL5/RPL11 play an essential role in normal cell proliferation, a function cells have evolved to rely on in lieu of a cell cycle checkpoint. (nih.gov)
  • Functional analysis revealed that knockdown of PTGR1 significantly inhibited proliferation and colony formation by PC cells. (go.jp)
  • Results demonstrated that pristimerin was toxic against the two cell lines, and exhibited inhibitory effects against proliferation. (spandidos-publications.com)
  • The expression of cyclin-dependent kinase inhibitors was profoundly affected with early activation and then repression of p21 cip1/waf1 and persistent activation of both p27 kip1 and p57 kip2 , whereas genes involved in cell survival and proliferation were suppressed. (aacrjournals.org)
  • 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-test was used to observe the proliferation of human breast cancer MCF-7 and MDA-MB-231 cells induced on VALD-3. (researchsquare.com)
  • Our study demonstrates that survivin and APE1/Ref-1 are significantly higher in human prostate cancer specimens compared to noncancerous controls and that APE1/Ref-1 redox-specific inhibition with small molecule inhibitor, APX3330 and a second-generation inhibitor, APX2009, decreases prostate cancer cell proliferation and induces cell cycle arrest. (oncotarget.com)
  • Glucose-6 phosphatase catalytic subunit inhibits the proliferation of liver cancer cells by inducing cell cycle arrest]. (bvsalud.org)
  • To investigate the effects of glucose-6-phosphatase catalytic subunit (G6PC) recombinant adenovirus on proliferation and cell cycle regulation of liver cancer cells . (bvsalud.org)
  • Cell proliferation and clone formation assay were used to observe the proliferation of liver cancer cells . (bvsalud.org)
  • Cell cycle flow cytometry assay was used to analyze the effect of G6PC overexpression on the proliferation cycle of liver cancer cells . (bvsalud.org)
  • Huh7 and SK-Hep1 cells proliferation assay showed that the number of proliferating cells in the AdG6PC group was significantly lower than the other two groups (P (bvsalud.org)
  • Western blot result showed that G6PC overexpression had down-regulated the proliferation in cell -cycle related proteins expression. (bvsalud.org)
  • G6PC inhibits the proliferation, cell -cycle related expression, and migration of liver cancer cells by inhibiting the G(1)/ S phase transition. (bvsalud.org)
  • Induced cell cycle arrest is the use of a chemical or genetic manipulation to artificially halt progression through the cell cycle. (wikipedia.org)
  • The cells can be induced to arrest as they arrive (at different time points) at a certain phase, so that when the arrest is lifted (for instance, rescuing cell cycle progression by introducing another chemical) all the cells resume cell cycle progression at the same time. (wikipedia.org)
  • Mimosine is a plant amino acid that has been shown to reversibly inhibit progression beyond G1 phase in some human cells, including lymphoblastoid cells. (wikipedia.org)
  • The use of serum deprivation - partially or completely removing the serum and its nutrients - has been shown to arrest and synchronize cell cycle progression in G0 phase, for example in neonatal mammalian astrocytes and human foreskin fibroblasts. (wikipedia.org)
  • It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)
  • Unexpectedly, we found that their depletion in primary human lung fibroblasts failed to induce cell cycle arrest but strongly suppressed cell cycle progression. (nih.gov)
  • We show that the effects on cell cycle progression stemmed from reduced ribosome content and translational capacity, which suppressed the accumulation of cyclins at the translational level. (nih.gov)
  • We studied the effect of doxorubicin (Dox) on cell cycle progression and its correlation with DNA damage and cytotoxicity in p53-mutant P388 cells. (aspetjournals.org)
  • In untreated control P388 cells, p34cdc2 kinase localizes in the nucleus and cytoplasms, particularly in the centrosomes, and p34cdc2 kinase activity is dependent on cell cycle progression, with the enzyme activity increasing steadily from G1/S to G2/M and markedly declining thereafter. (aspetjournals.org)
  • Actually, because effective depletion of most PPP2R5 subunits must halt cell routine progression, they are not really Meclofenoxate HCl paradoxes in any way. (estme.org)
  • Cell cycle flow cytometry showed that G6PC overexpression had significantly inhibited the Huh7 cells G(1)/ S phase transition. (bvsalud.org)
  • The effects of NBPF1 on the cell cycle were evaluated by flow cytometry. (biomedcentral.com)
  • A ) Active caspase-3 expression was detected by flow cytometry in HeLa, SiHa, and ME-180 cells after treatment with IC 25 and IC 50 of MNBE for 24 h. (biomedcentral.com)
  • Television producer Eric Weinberg, shown in this photo provided by the Los Angeles Police Department, was arrested on July 14, 2022. (wgntv.com)
  • We investigated the effects of various concentrations of Tan IIA (5-10 μ g/mL) on mouse keratinocytes and human HaCat cells in vitro to confirm this hypothesis. (hindawi.com)
  • Moreover, treatment with TCEE is found to enhance the cytotoxic effects of amphotericin B in human colon cancer cell both in vitro and in vivo [ 17 ]. (hindawi.com)
  • In vitro ubiquitination assay showed a decreased p27 ubquitination in PA-treated prostate cancer cells. (iiarjournals.org)
  • The effect of Colcemid on the in vivo system of regenerating rat liver and on the in vitro system of HeLa cell cultures was studied to determine some of the morphological and kinetic aspects of metaphase blockage and recovery. (rupress.org)
  • Because APE1/Ref-1 is expressed and elevated in prostate cancer, we sought to characterize APE1/Ref-1 expression and activity in human prostate cancer cell lines and determine the effect of selective reduction-oxidation (redox) function inhibition on prostate cancer cells in vitro and in vivo . (oncotarget.com)
  • While in G1 the cell synthesizes messenger RNA (mRNA) and proteins in preparation for subsequent steps of interphase leading to mitosis. (wikipedia.org)
  • The expression of pro-apoptotic proteins and anti-apoptotic proteins in MCF-7 and MDA-MB-231 cells was investigated by Western blotting. (researchsquare.com)
  • Antagonism of PP2A by HIV-1 Vif is normally unbiased of APOBEC3 family members proteins as a result, and regulates cell routine development in HIV-infected cells. (estme.org)
  • In addition, proteome analysis of NBPF1-overexpressing DLD1 cells identified 32 differentially expressed proteins, of which several are implicated in carcinogenesis. (biomedcentral.com)
  • We demonstrated that NBPF1 exerts different tumor suppressive effects, depending on the cell line analyzed, and provide new clues into the molecular mechanism of the enigmatic NBPF proteins. (biomedcentral.com)
  • The sodium channels containing proteins produced from the SCN5A gene are abundant in heart (cardiac) muscle cells and play key roles in these cells' ability to generate and transmit electrical signals. (medlineplus.gov)
  • Channels made with these altered SCN5A proteins stay open longer than usual, which allows sodium ions to continue flowing into cardiac muscle cells abnormally. (medlineplus.gov)
  • This study adds a PPP-family protein phosphatase to the proteins involved in the control of polar growth and cell shape determination in S. coelicolor. (lu.se)
  • To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)
  • Meanwhile, Maduro said Thursday afternoon that he would not hesitate to arrest members of "terrorist groups" in Venezuela after Chavista authorities detained Marrero and accused him of heading a terrorist cell. (laoferta.com)
  • It is an artificial activation of naturally occurring cell cycle checkpoints, induced by exogenous stimuli controlled by an experimenter. (wikipedia.org)
  • Police asked for records from four cell phone carriers on losses in their stores from the beginning of March 2023, when a former employee said thefts began, to just before the first arrests in July. (texomashomepage.com)
  • Seun Kuti revealed his decision to renovate the Panti cell in a video that quickly went viral on October 7, 2023. (notjustok.com)
  • Police in England arrested a man Tuesday, Nov. 14, 2023, on suspicion of manslaughter in the death of American ice hockey player Adam Johnson, whose neck was cut by a skate during a game. (kfor.com)
  • Moreover, the results clearly showed that kaempferol causes a strong inhibition of the activation of the EGFR/p38 signaling pathways, upregulation of p21 expression and downregulation of cyclin B1 expression in human RCC cells, together with activation of PARP cleavages, induction of apoptotic death and inhibition of cell growth. (nih.gov)
  • The induction of cell cycle arrest by a pheromone, chemically distinct from the one used to attract the opposite mating type, highlights the existence of a sophisticated mechanism to increase chances of mate finding, while keeping the metabolic losses associated with the release of an attraction pheromone to a minimum. (ugent.be)
  • Because of the patient's risk status, the physicians intended to perform allogeneic stem cell transplantation after induction and consolidation chemotherapy, which was scheduled to end in January 2013, and a conditioning chemotherapy regimen, which was planned to be given in March. (cdc.gov)
  • Phosphine is a respiratory tract irritant that attacks primarily the cardiovascular and respiratory systems causing peripheral vascular collapse, cardiac arrest and failure, and pulmonary edema. (cdc.gov)
  • These mutations alter the structure of ion channels made with the SCN5A protein and disrupt the flow of sodium ions into cardiac muscle cells. (medlineplus.gov)
  • This condition alters the normal beating of the heart and can lead to fainting (syncope) or sudden cardiac arrest and death. (medlineplus.gov)
  • Cardiac cells with these altered channels have difficulty producing and transmitting electrical signals that coordinate normal heartbeats. (medlineplus.gov)
  • The impaired cardiac cells die, leading to a buildup of scar tissue (fibrosis) over time that worsens the heart block. (medlineplus.gov)
  • Vasoconstriction, which occurs with hypothermia, may mask hypovolemia, which then manifests as sudden shock or cardiac arrest during rewarming (rewarming collapse) when peripheral vasculature dilates. (msdmanuals.com)
  • The decreased demand probably accounts for the occasional survival after prolonged cardiac arrest due to extreme hypothermia. (msdmanuals.com)
  • The ethanolic extract of T. camphoratus (TCEE) which contains abundant bioactive compounds including triterpenoids and polysaccharides also has antitumor effects in various human cancer cell lines. (hindawi.com)
  • The aims of this study are to clarify the antitumor effects of TCEE on human hepatocellular carcinoma cells and also evaluate the combination drug effects with conventional chemotherapy agents, cisplatin and doxorubicin. (hindawi.com)
  • In contrast, the antitumor effects and related biological mechanism of TCEE as well as the combination drug effects with conventional chemotherapy agents remain unclear particularly in human hepatocellular carcinoma cells. (hindawi.com)
  • The aims of this preclinical study are to evaluate the capability of TCEE to suppress human hepatocellular carcinoma cells and clarify the related antitumor effects. (hindawi.com)
  • however, the antitumor effects and underlying mechanisms of pristimerin in oral cancer cells have not yet been identified. (spandidos-publications.com)
  • In the present study, the potent antitumor effects of pristimerin on OSCC cells were investigated. (spandidos-publications.com)
  • NK cells are specialized effectors of the innate immune system that destroy their targets by antibody-dependent cell-mediated cytotoxicity, have prominent antitumor effects, and are potent killers of virally infected cells. (medscape.com)
  • Furthermore, the combined drug effects of TCEE with conventional chemotherapy agents, cisplatin and doxorubicin, were also analyzed to clarify whether TCEE enhances or antagonizes the cytotoxicity of the selected chemotherapy agents in hepatocellular carcinoma cells. (hindawi.com)
  • In this study, by comparing the activity of normal cell lines and cancer cell lines after treating with resveratrol, it was found that resveratrol has more significant cytotoxicity in cancer cell lines. (mdpi.com)
  • Cell cycle-dependent cytotoxicity, G2/M phase arrest, and disruption of p34cdc2/cyclin B1 activity induced by doxorubicin in synchronized P388 cells. (aspetjournals.org)
  • Our results suggest that anthracycline-induced cytotoxicity is cell cycle dependent and is mediated, at least in part, by disturbance of the regulation of p34cdc2/cyclin B1 complex, thus leading to G2/M phase arrest. (aspetjournals.org)
  • Here we show that methanolic extracts of Sapota fruit (MESF) induces cytotoxicity in a dose-dependent manner in cancer cell lines. (nature.com)
  • In a preliminary study it has been shown that phenolic antioxidants such as methyl 4- O -galloylchlorogenate and 4- O -galloylchlorogenic acid derived from Sapota fruits can induce cytotoxicity in colon cancer cells 23 . (nature.com)
  • This is one of the first occurrences in the nation where suspects were immediately arrested by law enforcement after allegedly introducing contraband into a prison facility. (michigan.gov)
  • Cell was allegedly in contact with extremists in Syria and targeted young people to fight for Isis. (ibtimes.co.uk)
  • The cell was allegedly linked to another three person recruitment cell arrested in February. (ibtimes.co.uk)
  • It is to be noted that last week the Cyber police team had also arrested Ishtiyaq Ansari, a tractor driver, for allegedly providing personal details of his customers to the cyber fraudster. (organiser.org)
  • Earlier, in a similar incident, a team of Mumbai police, with the help of local cyber police, had arrested as many as three accused, identified as Gulzaar, Saeed and Sarfan Ansari, all residents of Jamtara district for allegedly stealing around 1.8 lakh from a 70-year-old senior citizen's account in April this year. (organiser.org)
  • In another incident, a team of Delhi police had also raided Nawadih village in Naya Tola of Jamtara in April this year and arrested as many as six accused for allegedly running a fake customer care centre and duping around 2500 people across the country. (organiser.org)
  • A German tourist has been arrested for allegedly trying to smuggle four endangered iguanas out of the Galapagos Islands, according to the national park. (phys.org)
  • M: A mutant screen of budding yeasts with mitotic arrest identified CDC16, CDC23, and CDC27 as key genes that, when mutated, cause arrest in mitosis. (wikipedia.org)
  • We investigated by real-time quantitative RT-PCR the expression profile of a panel of genes important in cell cycle regulation. (biomedcentral.com)
  • Modulation of pro and anti-apoptotic genes in cervical cancer cells after exposure to MNBE. (biomedcentral.com)
  • Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)
  • Bingham was arrested without incident and booked into the Hillsborough County Jail. (ospreyobserver.com)
  • In that case, the court authorized police officers to search any person's cell phone after they had been arrested under a narrow exception to the Fourth Amendment's warrant requirement that permits law enforcement officers to search the area immediately around a person "incident to arrest. (eff.org)
  • In an amicus brief (pdf) recently filed before the Oregon Supreme Court, EFF argued that warrantless searches of cell phones incident to arrest violate the Constitution's right to privacy. (eff.org)
  • In cell culture, serum is the growth medium in which the cells are grown and contains vital nutrients. (wikipedia.org)
  • In the present study, we investigated the effects of kaempferol and the underlying mechanism(s) on the cell growth of RCC cells. (nih.gov)
  • Thus, the secretion of gall wasps stimulates plant cell growth and overrules homeostasis of the affected leaf area - this is similar to tumour outgrowth. (nature.com)
  • In turn, the plant produces GA, which seems to combat the improper growth signals and re-establishes cell-cycle control. (nature.com)
  • In the present study, the TCEE treatment induced cell cycle arrest and suppressed cell growth on both Hep3B and HepJ5 cells. (hindawi.com)
  • Partial pneumonectomy of telomerase null mice carrying shortened telomeres initiates cell growth arrest resulting in a limited compensatory growth response. (ca.gov)
  • In B6.Cg-Terc(tm1Rdp) mice carrying shortened telomeres, response to pneumonectomy was characterized by decreased survival, diminished compensatory lung growth, attenuated distal lung progenitor cell response, persistent DNA damage, and cell growth arrest. (ca.gov)
  • DIM treatment inhibited the growth of SKOV-3, TOV-21G, and OVCAR-3 ovarian cancer cells in both a dose- and time-dependent manner with effective concentrations ranging from 40 to 100 μM. (aspetjournals.org)
  • Growth-inhibitory effects of DIM were mediated by cell cycle arrest in G 2 /M phase in all the three cell lines. (aspetjournals.org)
  • By xenografting human breast cancer cells into nude mice, we found that VALD-3 significantly suppressed tumor cell growth while showing low toxicity against major organs. (researchsquare.com)
  • Due to the role of oncogenic transcriptional activators NFĸB and STAT3 in survivin protein expression, and APE1/Ref-1 redox activity regulating their transcriptional activity, we assessed selective inhibition of APE1/Ref-1's redox function as a novel method to halt prostate cancer cell growth and survival. (oncotarget.com)
  • Decreased expression of NBPF1 in neuroblastoma cell lines with loss of 1p36 heterozygosity and the marked decrease of anchorage-independent clonal growth of DLD1 colorectal carcinoma cells with induced NBPF1 expression further suggest that NBPF1 functions as tumor suppressor. (biomedcentral.com)
  • Members of the Actinobacteria, including mycobacteria and streptomycetes, exhibit a distinctive mode of polar growth, with cell wall synthesis occurring in zones at cell poles and directed by the essential cell polarity determinant DivIVA. (lu.se)
  • Here, we show that the phosphoprotein phosphatase SppA has strong effects on polar growth and cell shape and that it reverses the AfsK-mediated phosphorylation of DivIVA. (lu.se)
  • The sppA mutant hyphae also exhibit a high frequency of spontaneous growth arrests, indicating problems with maintenance of tip extension. (lu.se)
  • This natural process can be mimicked in a lab through the overexpression of p27Kip1, which results in induced cell cycle arrest in G1 phase. (wikipedia.org)
  • We have demonstrated that phenylacetate(PA)-induced cell cycle arrest in human prostate cancer is mediated by increase of p27. (iiarjournals.org)
  • Frog egg cell extracts have been used extensively in cell cycle research because they are relatively large, reaching a diameter of 1mm, and so contain large amounts of protein, making protein levels more easily measurable. (wikipedia.org)
  • p27Kip1 protein levels are elevated in arresting cells. (wikipedia.org)
  • Palestinian security forces have arrested 25 Hamas terrorists in the West Bank, mostly in the Ramallah region. (ynetnews.com)
  • In the past month Israeli security forces arrested multiple Hamas members, who attended Birzeit University in the West Bank, the Shin Bet (Israel Security Agency) cleared for publication on Sunday. (jpost.com)
  • We report Mycetohabitans rhizoxinica bacteremia in a 65-year-old woman in California, USA, who was undergoing chimeric antigen receptor T-cell therapy for multiple myeloma. (cdc.gov)
  • A 65-year-old woman in California, USA, with a history of relapsed and refractory multiple myeloma visited a hospital to receive chimeric antigen receptor (CAR) T-cell therapy. (cdc.gov)
  • Imaging studies from a 65-year-old woman with multiple myeloma undergoing chimeric antigen receptor T-cell therapy who was admitted to the hospital for worsening foot and ankle pain, California, USA. (cdc.gov)
  • The underlying molecular mechanisms by which androgen-independent PC cells acquire the capacity to proliferate remain largely unclear. (go.jp)
  • Two PTGR1-specific short hairpin RNA (shRNA) sequences were used to block the expression of PTGR1 via a lentivirus-mediated system in the androgen-independent PC cell lines DU145 and PC 3. (go.jp)
  • In this study, we further investigated the mechanism of PA-induced p27 expression in prostate cancer cells (LNCaP, androgen-independent LNCaP [AIDL] and PC-3). (iiarjournals.org)
  • As Indonesia gears up for crucial elections, slated to include the presidential election on February 14, 2024, these arrests highlight the persistent threat posed by extremist groups like Jemaah Islamiyah and JAD. (thebalitimes.com)
  • It results in a single layer of arrested cells of arrested cells, and is a process that is notably missing in cancer cells. (wikipedia.org)
  • Furthermore, di-GA inhibited the generation of lymphendothelial gaps by cancer cell spheroid-secreted lipoxygenase metabolites. (nature.com)
  • We evaluated the effect of 3,3′-diindolylmethane (DIM) in ovarian cancer cells. (aspetjournals.org)
  • These results clearly indicate the requirement of Chk2 activation to cause G 2 /M arrest by DIM in ovarian cancer cells. (aspetjournals.org)
  • Our results establish Chk2 as a potent molecular target of DIM in ovarian cancer cells and provide the rationale for further clinical investigation of DIM. (aspetjournals.org)
  • In this study, we investigated the cytotoxic and apoptotic effects of VALD-3, a Schiff base ligand synthesized from o-vanillin derivatives, on human breast cancer cells and the possible underlying mechanisms. (researchsquare.com)
  • VALD-3 also regulated the Wnt/β-catenin signaling pathway in breast cancer cells, inhibiting the activation of downstream molecules. (researchsquare.com)
  • A striking decrease in Skp2 mRNA and protein expression and reciprocal increase in p27 protein level were observed in three PA-treated prostate cancer cells. (iiarjournals.org)
  • Our results suggest that PA attenuated Skp2 expression, thereby inhibiting ubiquitination and promoting p27 accumulation in all three prostate cancer cell lines. (iiarjournals.org)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Transwell and scratch assay were used to observe the invasion and migration of liver cancer cells . (bvsalud.org)
  • Western blot was used to detect the effect of G6PC overexpression on the cell -cycle protein expression in liver cancer cells . (bvsalud.org)
  • Clone formation assay showed that the number of clones was significantly lower in AdG6PC than the other two groups (P liver cancer cells . (bvsalud.org)
  • Scratch repair rate was significantly lower in AdG6PC than the other two groups (P liver cancer cells . (bvsalud.org)
  • LC-MS/MS analysis was used to investigate the proteome of DLD1 colon cancer cells with induced NBPF1 expression. (biomedcentral.com)
  • Transwell assay showed that the number of cell migration was significantly lower in AdG6PC than the other two groups (P (bvsalud.org)
  • Protein activation and expression were analysed by western blotting, deoxyribonucleoside triphosphate levels by HPLC, ribonucleotide reductase activity by 14 C-cytidine incorporation into nascent DNA and cell-cycle distribution by FACS. (nature.com)
  • Forced expression of NBPF1 in HEK293T cells resulted in a G1 cell cycle arrest that was accompanied by upregulation of the cyclin-dependent kinase inhibitor p21 CIP1/WAF1 in a p53-dependent manner. (biomedcentral.com)
  • Additionally, forced expression of NBPF1 in two p53-mutant neuroblastoma cell lines also resulted in a G1 cell cycle arrest and CDKN1A upregulation. (biomedcentral.com)
  • B ) Western blot analysis of the expression of NFkB-p65 and p53 in HeLa, SiHa, and ME-180 cells treated with MNBE at indicated dose for 24 h. (biomedcentral.com)
  • immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)
  • B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)
  • α-factor is a pheromone secreted by Saccharomyces cervisiae that arrests the yeast cells in G1 phase. (wikipedia.org)
  • Here, we report transmission of dengue virus to a peripheral blood stem cell recipient by a donor who had recently traveled to an area to which the virus is endemic. (cdc.gov)
  • It said the cell was dedicated to "the recruitment and radicalization of young people and children into the ranks of the terrorist organization Daesh (a pejorative term for Isis). (ibtimes.co.uk)
  • Venezuela's interior minister on Thursday accused the detained chief of staff of opposition leader and self-proclaimed interim head of state Juan Guaido of leading a terrorist cell that was planning to sow chaos with targeted attacks. (laoferta.com)
  • Reverol said intelligence services had "once again dismantled a terrorist cell that was planning to carry out a series of targeted attacks and to that end had hired Colombian and Central American mercenaries. (laoferta.com)
  • Bahrain security forces arrested several members of a terrorist cell in Manama on Monday. (geopolitical.report)
  • The Iranian authorities announce arresting a terrorist cell that was planning to carry out sabotage operations inside the country, and the Iranian army announces its readiness to confront "the various plots hatched by the enemies. (almayadeen.net)
  • Border Guard Commander in East Azerbaijan Province, Muhammad Ahmadi, announced arresting a terrorist cell in the province located in western Iran. (almayadeen.net)
  • Colonel Ahmadi confirmed that "two elements of the terrorist cell were planning to carry out sabotage operations inside the country, and they found in their possession a number of weapons and munition," adding, "Within this operation, two cars carrying various military weapons, cameras, and remote explosives were stopped. (almayadeen.net)
  • Zhao H , Halicka HD , Li J , Darzynkiewicz Z , . Berberine suppresses gero-conversion from cell cycle arrest to senescence. (aging-us.com)
  • Abu Dhabi: Plans for a Biobank of stem cells and tissues, for use in advanced treatments, have been approved in Abu Dhabi. (gulfnews.com)
  • The plan was presented by the Department of Health - Abu Dhabi for the project, which supports the provision of precision and personalised medicine through advanced treatment programmes that use human stem cells and tissues to develop medical research. (gulfnews.com)
  • Hematopoietic stem cell transplantation has become a major treatment option for patients with hematopoietic malignancies and immune deficiencies. (cdc.gov)
  • To the best of our knowledge, only the transmission of malarial parasites has been reported during stem cell transplantation. (cdc.gov)
  • The stem cell mobilization result was poor. (cdc.gov)
  • Furthermore, pristimerin exhibited a more potent anti‑proliferative activity in CAL‑27 and SCC‑25 cells than the common chemotherapy drugs cisplatin and 5‑fluorouracil. (spandidos-publications.com)
  • In the present study, the anticancer potential of pristimerin was investigated in two oral squamous cell carcinoma (OSCC) cell lines, CAL‑27 and SCC‑25. (spandidos-publications.com)
  • Silencing of the EBF3 locus was observed in brain, colorectal, breast, liver, and bone tumor cell lines and its reactivation was achieved on treatment with 5-aza-2′-deoxycytidine and trichostatin A in a significant portion of these tumor cells. (aacrjournals.org)
  • G 2 /M arrest was associated with DNA damage as indicated by phosphorylation of H 2 A.X at Ser139 and activation of checkpoint kinase 2 (Chk2) in all the three cell lines. (aspetjournals.org)
  • each group of cell lines was established. (lu.se)
  • cells are activated by interaction with antigens and obtain T indicated that the cell lines representing a defined cell help, they mature into terminally differentiated plasma stage generally presented a high similarity in over- cells secreting large amounts of antibodies [10 -12]. (lu.se)
  • The use of cell lines does, however, introduce a risk the immunoglobulin (Ig)-secreting plasma cell in the spleen, of obtaining cell line-specific features as a result of the trans- gut, or BM [1, 2]. (lu.se)
  • Renal cell dysfunction and decreased levels of vasopressin (ADH) lead to production of a large volume of dilute urine (cold diuresis). (msdmanuals.com)
  • The City of New Haven, Connecticut, fired two police officers Wednesday for what authorities called their reckless actions and lack of compassion toward Richard "Randy" Cox, who was injured and became paralyzed in the back of a police van after his arrest last year. (wfla.com)
  • The underlying mechanisms of these effects were primarily mediated by G 1 phase cell cycle arrest and inhibition of the mitogen-activated protein kinase (MAPK)/extracellular signal-regulate kinase 1/2 (Erk 1/2) and protein kinase B (Akt) signaling pathways. (spandidos-publications.com)
  • Another reason synchrony is important is the control for amount of DNA content, which varies at different parts of the cell cycle based on whether DNA replication has occurred since the last round of completed mitosis and cytokinesis. (wikipedia.org)
  • In human somatic cells, the cell cycle lasts about 18 hours, and the G1 phase makes up about 1/3 of that time. (wikipedia.org)
  • Individual HeLa cells were followed by time-lapse cinemicrography prior to, during, and after Colcemid treatment. (rupress.org)
  • Cells entered mitosis at a normal rate and passed through prophase. (rupress.org)
  • The generation of receptors specific for antigens is a unique and complex process that generates 10 12 specific receptors for each cell type of the adaptive immune system, including T and B cells. (medscape.com)
  • Various cell types can process and present these antigens to T cells, or antigens may be soluble and bound to B-cell receptors. (medscape.com)
  • G1 phase is the first of the four phases of the cell cycle, and is part of interphase. (wikipedia.org)
  • There was no apparent effect on cells in interphase. (rupress.org)
  • In some experiments, a researcher may want to control and synchronize the time when a group of cells progress to the next phase of the cell cycle. (wikipedia.org)
  • Furthermore, synchronization of large numbers of cells into the same phase allows for the collection of large enough groups of cells in the same cycle for the use in other assays, such as western blot and RNA sequencing. (wikipedia.org)
  • Examples include: G1: Saccharomyces cerevisiae yeast expressing dominant mutant alleles of CDC28 arrest in G1, which indicates that CDC28 is necessary for passage beyond the G1 phase. (wikipedia.org)
  • S: Schizosaccharomyces pombe (fission yeast) expressing a temperature-sensitive mutant form of DNA polymerase delta (pol delta ts03) arrest in S phase. (wikipedia.org)
  • Treatment of synchronized cells in early S phase with 1 microM Dox (2 x ID50) for 1 hr induced a marked cell arrest at G2/M phase at 6-12 hr after drug incubation. (aspetjournals.org)
  • Interestingly, reduction of phospho-Akt and up-regulation of p27 mRNA levels were observed only in PC-3 cells. (iiarjournals.org)
  • Cellular processes like genome duplication and cell division stop. (wikipedia.org)
  • Other cellular components of the innate immune system include mononuclear phagocytes, dendritic cells, and NK cells. (medscape.com)
  • Natural-killer (NK) cells are specialized lymphocytes that have cytotoxic properties in addition to their ability to produce cytokines that assist in the orchestration of adaptive immunity. (medscape.com)
  • The adaptive immune system consists of 2 types of lymphocytes: T cells (70-75% of the adaptive immune force) and B cells (10-20% of the adaptive immune force). (medscape.com)