• Oral administration of GTE reduces significantly infiltration of inflammatory cells and exudation of proteins in the aqueous humor, which are associated with reduced expression and secretion of proinflammatory factors in the ciliary body and iris 13 . (nature.com)
  • Cellular agriculture focuses on the production of agricultural products from cell cultures using a combination of biotechnology, tissue engineering, molecular biology, and synthetic biology to create and design new methods of producing proteins, fats, and tissues that would otherwise come from traditional agriculture. (wikipedia.org)
  • On the basis of type it includes Blood, Serum, Plasma, and Animal Proteins, Tissue and Tissue Extracts and others. (researchandmarkets.com)
  • A DINOSAUR bone buried for 80 million years has yielded a mix of proteins and microstructures resembling cells. (com.pk)
  • Pulmonary function, bronchoalveolar lavage cell differentials, plasma immunoglobulin (Ig) protein levels, and lung tissue pathology, cyto-/chemo-kine proteins, and gene expression were assessed on day 15. (cdc.gov)
  • reported that the quality of the genomic DNA for next-generation sequencing (NGS) is better preserved in LBC samples than in formalin-fixed paraffin embedded (FFPE) tissues even after several years of storage ( 15 ). (frontiersin.org)
  • The Prosigna test utilizes RNA extracted from macrodissected tumor cells obtained from formalin-fixed, paraffin-embedded (FFPE) tissue sections. (lu.se)
  • Material and Method: Paraffin-embedded blocks from 30 patients with oropharynx squamous cell carcinomas, diagnosed and treated at the Oral Oncology Center were selected. (bvsalud.org)
  • Conclusion: Dilution of the DNA extracted of paraffin-embedded materials did not modify statistically the amount of positive samples β-globin gene amplified in PCR, although the results suggest that this is a way to increase the method for efficacy amplification of PCR. (bvsalud.org)
  • This research was done to study the influence of Acalypha indica Linn root extract towards relative cell viability and proliferation as parameters of neurogenesis in post-hypoxic hippocampal tissue culture. (ui.ac.id)
  • After 90 hours of incubation, relative cell viability was measured by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) examination, and cell proliferation was measured by using 5-bromo2-deoxy-uridine (BrdU) for cell proliferation. (ui.ac.id)
  • Cell proliferation of rat hippocampal tissue culture treated with Acalypha indica Linn root extract with dose of 10, 15, and 20 mg/mL was significantly higher than control (0.132, 0.117, 0.114 vs 0.096). (ui.ac.id)
  • Acalypha indica Linn root extract with doses of 10, 15, and 20 mg/mL can increase relative cell viability and proliferation in post-hypoxic hippocampal tissue culture. (ui.ac.id)
  • It is likely that two different media formulations will be required for each cell type: a proliferation media, for growth, and a differentiation media, for maturation. (wikipedia.org)
  • Potential synergistic effects of various combinations including these compounds were tested by measuring cancer cell proliferation and apoptosis. (frontiersin.org)
  • Furthermore, the proliferation of Th17 cells and Th17‑associated cytokines, including interleukin (IL)17, IL23 and RAR‑related orphan receptor γt, was analyzed by reverse transcription‑quantitative polymerase chain reaction. (spandidos-publications.com)
  • In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)
  • The ectopic overexpression of TC2N accelerated the proliferation, migration, and invasion of HCC cells, whereas its knockdown showed the opposite effects. (bvsalud.org)
  • Building on maturation, and T cell proliferation would cover the critical the previous development of a time course in vitro human skin steps leading to induction. (cdc.gov)
  • A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
  • The ideal criteria for cell lines for the purpose of cultured meat production include immortality, high proliferative ability, surface independence, serum independence, and tissue-forming ability. (wikipedia.org)
  • Conventional methods for growing animal tissue in culture involves the use of fetal bovine serum (FBS). (wikipedia.org)
  • After the creation of the cell lines, efforts to remove serum from the growth media are key to the advancement of cellular agriculture as fetal bovine serum has been the target of most criticisms of cellular agriculture and cultured meat production. (wikipedia.org)
  • Where polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from the tissue culture supernatants of transfected host cell lines. (cellsignal.com)
  • The American College of Obstetricians and Gynecologists (ACOG) has recommended that serum screening, cell-free DNA screening, and diagnostic tests, such as chorionic villus sampling (CVS) and amniocentesis , be discussed with and offered to all women early in pregnancy, regardless of their age. (medscape.com)
  • To determine the serum electrolytes levels in animals treated with Catha edulis leaf extract. (ajol.info)
  • The supernatants from the cells were examined by electron microscopy, and a coronavirus was identified. (cdc.gov)
  • Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
  • This is evidenced by the fact that established protocols for creating human and mouse embryonic stem cells have not succeeded in establishing ungulate embryonic stem cell lines. (wikipedia.org)
  • But the story has taken a new twist as religious groups are now beginning to discourage followers from participating -- because ALS research at times relies on embryonic stem cells. (salon.com)
  • This week, the Archdiocese of Cincinnati specifically asked Catholic school leaders at 113 schools to "immediately cease" any fundraising plans connected to the challenge, because the ALS Association funds at least one study using embryonic stem cells -- "in direct conflict with Catholic teaching. (salon.com)
  • She added that donors "can stipulate where their money goes and can ask that it not pay for embryonic stem cell research. (salon.com)
  • The Archdiocese suggests people who want to do the challenge contribute to the John Paul II Medical Research Institute, "where the research is only conducted using adult stem cells. (salon.com)
  • Researchers investigating many other conditions, including Parkinson's , also have been known to use embryonic stem cells. (salon.com)
  • there are reasons to have conversations about embryonic stem cell research and fetal tissue research. (salon.com)
  • Stem cell therapy is a completely different process of extracting rejuvenating cells. (prweb.com)
  • Due to stem cells' ability to restore and regenerate, their application toward pain relief is a logical step. (prweb.com)
  • Professor Post is leading the way in the race to grow meat from stem cells rather than factory-farmed animals. (abc.net.au)
  • They include plant-based food company Hampton Creek, founded by a Humane Society executive, and Memphis Meats, started by a cardiologist and a stem cell scientist. (abc.net.au)
  • Cornea is constantly regenerated from a small population of limbal epithelial stem cells via transiently amplifying cell (TAC) states. (stanford.edu)
  • Hematopoietic stem cell (HSC) replacement transplantation can ameliorate diseased hematologic system, thus inhibiting immune disorders including autoimmune diseases and immunodeficient disorders. (stanford.edu)
  • The preliminary study is aimed at evaluating the cytotoxic activity of crude extracts from the stem bark of Canarium odontophyllum against colorectal carcinoma cell HCT 116. (scirp.org)
  • Result of the extraction yield showed that distilled water produced the highest yield (10.58%) followed by methanol (6.55%) whereas acetone extracted the lowest yield (4.58%) from C. odontophyllum stem bark. (scirp.org)
  • Phytochemical screening test revealed the presence of terpenoid, flavonoid, tannin, saponin and phenolic compound in all the stem bark extracts of C. odontophyllum. (scirp.org)
  • Acetone extract of C. odontophyllum stem bark showed significantly higher (p (scirp.org)
  • In conclusion, acetone and methanol extracts from C. odontophyllum stem bark exhibited promising cytotoxic activity against human colorectal cancer cell line HCT 116 and paves way for further investigation in view of its mode of cancer cell death. (scirp.org)
  • Their findings indicate that postmortem tissue may be a potential source of multipotent stem cells, with a variety of uses and applications. (salk.edu)
  • Such a cell would be a neural stem cell by a strict definition. (salk.edu)
  • In addition to isolating single cells in order to determine if true stem cells are present in adult brain tissue, Gage also plans to transplant recovered cells into animals to test if the cells can survive and differentiate in vivo. (salk.edu)
  • Testing in whole animals is the only way to know if adult tissue can be a source of stem or progenitor cells for transplant purposes to treat neurodegenerative disease," said Gage. (salk.edu)
  • An adult source of stem cells would circumvent the need to derive such cells from fetal tissue, currently an issue of ethical debate. (salk.edu)
  • Scientists removed stem cells from the boy's bone marrow and modified them in the lab by introducing copies of a gene to prevent his red blood cells from becoming "sickled. (technologyreview.com)
  • They isolated skin stem cells and added copies of a healthy version of the gene. (technologyreview.com)
  • The experiment to treat Parkinson's is a critical early test of stem cells' potential to tackle serious disease. (technologyreview.com)
  • Neural stem cell-derived extracellular vesicles (NSC-EVs) are involved in repairing neuronal injury. (bvsalud.org)
  • Nobel Prize winner Alexis Carrel performed numerous experiments clearly showing that tissue explants, including connective tissue and heart tissue, could be cultured in vitro preserving their characteristics for prolonged periods of time [ 2 ] supporting the notion that entire organs could be cultured in vitro. (intechopen.com)
  • Experimental in vitro study using 24 primary neuronal cell cultures obtained from adult Sprague Dawley rat exposed to hypoxia with 5% O 2 /5% CO 2 /N 2 balance gas for 24 hours. (ui.ac.id)
  • Preliminary in vitro evaluation of the antidiabetic activity of crude extract and its furanocoumarins was carried out ( α -amylase, α -glucosidase, and β -galactosidase). (hindawi.com)
  • Estimates of majority of the data considered were measures of reactivity, the uncertainty, as well as average values of the epidermal both purely chemical and in vitro cell based, whereas epidermal disposition kinetics parameters, were made by fitting to the time disposition was simply represented by the octanol-water course skin permeation data from individual skin donors. (cdc.gov)
  • Seven stable transformants were identified and evaluated for fluorescence in culture and during colonization, and were evaluated for colonization of host tissues. (usda.gov)
  • The neuronal apoptosis in hippocampal tissue were detected. (bvsalud.org)
  • RESULTS: CORT induction increased neuronal apoptosis in hippocampal tissue and induced depression-like behaviors in rats, while NSC-EV treatment improved depression-like behaviors and apoptosis in rats. (bvsalud.org)
  • Fluorescence was quantified directly from mycelium and protein extracted from hyphae. (usda.gov)
  • Immunoprecipitation of APP protein from mouse brain tissue extracts. (cellsignal.com)
  • The blood glucose level, liver function enzymes, total protein, lipid, and cholesterol levels were significantly normalized by extract treatment. (hindawi.com)
  • Specifically, information about epidermal disposition, step in human skin sensitization induction is the transport of sensitizer from the applied dose on the skin surface to the protein reactivity, epidermal inflammation, dendritic cell epidermis, where innate immune activation occurs. (cdc.gov)
  • Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.com)
  • Traditional polyclonal and monoclonal antibodies are the product of normal B cell development and genetic recombination. (cellsignal.com)
  • While polyclonal antibodies are secreted by many different B cell clones and recognize multiple antigenic epitopes, monoclonals originate from a single B cell clone and are specific for just one epitope. (cellsignal.com)
  • Histopathologic findings exhibited here, revealed a cellular phenomenon known as the transmigration of inflammatory cells, which explained the movement of antibodies into the intestinal lumen. (cdc.gov)
  • With a fluorescent reporter gene-expressing γHV68 we demonstrate that ABCs are infected with γHV68 at similar rates to other previously activated B cells. (nature.com)
  • These results indicate that HBO improves ischemic wound healing by downregulation of HIF-1alpha and subsequent target gene expression with attenuation of cell apoptosis and reduction of inflammation. (nih.gov)
  • We'll then extract their RNA and qPCR to analyze the markers via gene expression. (stanford.edu)
  • In murine mammary gland cells (NMuMG), Tβ4 upregulation is required for full induction of a MRTF-regulated EMT gene expression program after TGFβ stimulation. (aacrjournals.org)
  • PCBP1 is both reasonably highly expressed and has a low Gini coefficient in both tissues and cell lines, and is an excellent novel housekeeping gene. (biorxiv.org)
  • In March, researchers announced that a teenage boy in France had been cured of sickle-cell disease after receiving an experimental gene therapy developed by Bluebird Bio. (technologyreview.com)
  • When a bacterial infection threatened his life, a boy with a devastating connective tissue disorder called epidermolysis bullosa got new skin created with gene therapy . (technologyreview.com)
  • To in-vestigate the impact of the sample preparation approach on ROR scores, we analyzed 94 breast carcinomas included in an observational study that had available gene expression data from macro-dissected FFPE tissue and FF bulk tumor tissue, along with the clinically approved Prosigna scores for the node-negative, hormone-receptor-positive, HER2-negative cases (n = 54). (lu.se)
  • Since genes are composed of a given alphabet (A, T, C, G) with fixed pairing properties A-T and C-G, probes can be constructed that attract gene transcripts extracted from cell tissues and cultures. (lu.se)
  • Levels of gene expression are measured using a preparation of fluorescently labeled tissue RNA (copies of DNA), together with reference RNA labeled with a different fluorochrome, hybridized onto the slides. (lu.se)
  • Typically when data are presented, the fluor- escent intensity from the tissue is pseudo-colored red and the intensity from the reference green, and the logarithmic ratio of background corrected red and green intensities for each gene (spot) is subject to analysis (see Fig. 1 ). (lu.se)
  • Detection of North American West Nile virus in animal tissue by a reverse transcription-nested polymerase chain reaction assay. (cdc.gov)
  • In animal tissue it is possible to perform cross-sectional studies on the eruption process with the surrounding periodontal membrane and bone, both histologically and immunohistochemically. (hindawi.com)
  • Overall, ROR scores calculated based on the macro-dissected FFPE tissue were consistent with the Prosigna scores. (lu.se)
  • The diversity of receptors known to impact cAMP-mediated signaling includes those known to affect cardiovascular and nervous systems, immune mechanisms, cell growth, differentiation, and general metabolism. (enzolifesciences.com)
  • We think this type of tool will be quite useful to the community, as cell invasion is important in cancer and also a host of other (non-pathological) processes, like tissue growth, differentiation and repair," Wheeler said. (utoronto.ca)
  • Cells recovered from healthy individuals could provide a model for understanding how to stimulate and guide the normal processes of brain cell growth and differentiation," he said, "lending insight to how growth might be stimulated in people suffering from neurodegenerative diseases such as Alzheimer's or Parkinson's. (salk.edu)
  • Cluster of differentiation (CD) 4 + T cells are essential organizers of cell-mediated immunity, participating in each stage of the immune response. (spandidos-publications.com)
  • The perturbances in cellular redox homeostasis leads to high free radical burden thereby causing oxidative stress in the cells. (stanford.edu)
  • Staining of whole-mount retina showed that the number of activated microglia cells was significantly increased at 48 hours post-injection, which was suppressed after GTE treatment in a dose-dependent manner. (nature.com)
  • Analysis of ischemic wound tissue extracts demonstrated significantly reduced expression of HIF-1alpha, p53, and BNip3. (nih.gov)
  • However, TC2N expression was significantly upregulated in HCC tissues and hepatoma cell lines, and this upregulation was positively correlated with tumor progression in HCC patients. (bvsalud.org)
  • The present study investigated the expression of T helper (Th)17 cytokines in patients with laryngeal squamous cell carcinoma (LSCC) and their clinical significance in providing new therapeutic insights. (spandidos-publications.com)
  • 95% of all laryngeal malignancies are squamous cell carcinomas (SCCs) ( 2 ). (spandidos-publications.com)
  • Carcinoma, squamous cell. (bvsalud.org)
  • By 2014, IndieBio, a synthetic biology accelerator in San Francisco, has incubated several cellular agriculture startups, hosting Muufri (making milk from cell culture, now Perfect Day Foods), The EVERY Company (making egg whites from cell culture), Gelzen (making gelatin from bacteria and yeast, now Geltor), Afineur (making cultured coffee beans) and Pembient (making rhino horn). (wikipedia.org)
  • The bacteria build up in plaque, and, as the bacteria grow and die, they release lipids from their cell walls, which the immune system recognizes as signals of infection, triggering inflammation. (asbmb.org)
  • In addition to the puzzle of why mouth bacteria would have gut-protective lipids in their cell walls, Nichols soon found another: In periodontitis-diseased tissues, P. gingivalis was present, but not much LPS was evident in tissues. (asbmb.org)
  • Nichols didn't give up then, and has continued growing the bacteria, extracting the lipids, and screening their biological activity - and this has led to even more collaborations. (asbmb.org)
  • and a long-term accumulation of plaque (a filmlike substance made up primarily of bacteria, saliva, food debris, and dead cells that is continually being deposited on teeth) and tartar (hardened plaque) on the teeth and the gums. (msdmanuals.com)
  • The plaque and bacteria cause chronic inflammation that damages the tissue and bone that hold teeth in place. (msdmanuals.com)
  • We studied the effect of bilberry ( Vaccinium myrtillus L.) fruits extracts (BEs) on the pathogenesis caused by lipid accumulation in fatty liver and non-alcoholic steatohepatitis (NASH). (nih.gov)
  • 5 μg/ml of BEs was enough to suppress lipid accumulation in the fatty liver model of the mouse hepatic AML12 cells. (nih.gov)
  • Nichols wanted to know more about bacterial lipid triggers, so he started extracting and characterizing them. (asbmb.org)
  • Thanks to the genetic code perfectly preserved in the still intact blood cells, the scientists then clone a dinosaur. (forbes.com)
  • However, SC is very sensitive to insufficient cell counts, smears filled with inflammatory cells or blood cells, drying artifacts, crushing artifacts, or thick tissue fragments, which can mask cytological features and result in suboptimal diagnosis ( 5 ). (frontiersin.org)
  • When the treated cells were infused back into his body, they began to make normal blood cells. (technologyreview.com)
  • More than two years after treatment, the patient has enough normal red blood cells to evade any side effects of the disorder. (technologyreview.com)
  • Human Immunodeficiency Virus (HIV) Infection Human immunodeficiency virus (HIV) infection is a viral infection that progressively destroys certain white blood cells and is treated with antiretroviral medications. (msdmanuals.com)
  • Scientists were initially interested in somatic-cell nuclear transfer as a means of determining whether genes remain functional even after most of them have been switched off as the cells in a developing organism assume their specialized functions as blood cells, muscle cells, and so forth. (who.int)
  • EV uptake by PC12 cells was measured and PC12 cell apoptosis was detected. (bvsalud.org)
  • Functional rescue experiments were conducted to verify the role of miR-16-5p and MYB in PC12 cell apoptosis. (bvsalud.org)
  • In PC12 cells, NSC-EVs decreased CORT-induced PC12 cell apoptosis. (bvsalud.org)
  • miR-16-5p knockdown in EVs partially reversed the inhibitory effects of NSC-EVs on CORT-induced PC12 cell apoptosis. (bvsalud.org)
  • miR-16-5p targeted to inhibit MYB to repress CORT-induced PC12 cell apoptosis. (bvsalud.org)
  • However, efforts have been made to genetically transform mature citrus tissues but the efficiencies are generally extremely low (Marutani-Hert et al. (springer.com)
  • Called CAR-T therapies, these "living drugs" are made by extracting T cells from patients and genetically engineering them to go after and destroy cancer cells. (technologyreview.com)
  • Infection and colonization of tissues were clearly visible with a fluorescent microscope. (usda.gov)
  • We predict that ABCs could be playing a role throughout γHV68 infection due to their long-term persistence, activation of T cells, and continuous cytokine and antibody production. (nature.com)
  • B cells are also known to be important during latent γHV68 infection. (nature.com)
  • Periodontal disease is not like having a microbial infection around the teeth where the organisms invade the tissues and cause abscess formation, swelling, pain. (asbmb.org)
  • No cytotoxic effect was observed by the aqueous extract despite its high extraction yield. (scirp.org)
  • ChIP: HepG2 chromatin extract. (abcam.com)
  • Characterisation of the chromatin structures and dynamic mechanisms in human and murine cells and as well in the Malaria causing parasite Plasmodium falciparum. (uni-regensburg.de)
  • 4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
  • While some methods and protocols from human and mouse cell culture may apply to agricultural cellular materials, it has become clear that most do not. (wikipedia.org)
  • Despite their optimism to add this type of analysis of fossil DNA to more common methods - like DNA recovered from skeletal material, mummified and frozen tissues - the researchers have no intention of raising dinosaurs. (forbes.com)
  • There are differences in cell morphology, background, and artifacts between the ThinPrep and SurePath methods ( 19 , 20 ). (frontiersin.org)
  • METHODS: EVs were extracted from NSCs. (bvsalud.org)
  • In conclusion, Th17 cells have a substantial impact on the carcinogenesis of LSCCs, and could serve as a potential therapeutic target to modulate the anti‑tumor response in these carcinomas. (spandidos-publications.com)
  • In this study, we investigated a plausible therapeutic synergism of a triple combination of CBD/CBG, curcumin, and piperine in the colon adenocarcinoma using HCT116 and HT29 cell lines. (frontiersin.org)
  • Fat grafting also reduces allergic reactions and tissue rejections. (ukprwire.com)
  • Recently, severe asthma and allergic sensitization have been associated with T-helper cell type 17 (TH17) activation. (cdc.gov)
  • Results indicated low-dose PM2.5 extracts can enhance allergic sensitization and TH17-associated responses. (cdc.gov)
  • Given that the basic genome of a differentiated organism is constant between cells (and we here ignore epigenomics), what mainly discriminates one cell type from another is its expression profile. (biorxiv.org)
  • In an effort to advance the treatment strategies for the ocular surface diseases, I am focused on a regenerative medicine and single cell RNA sequencing based approaches to better comprehend the cellular biomarkers and molecular mechanisms involved in corneal tissue development and diseases. (stanford.edu)
  • We have previously demonstrated that Green Tea Extract (GTE) is a potent anti-inflammatory agent for anterior uveitis. (nature.com)
  • It is suggested that curcumin can selectively kill tumor cells through its multifaceted metabolic effects, that culminate in its anti-oxidant and anti-inflammatory activities ( Hewlings and Kalman, 2017 ). (frontiersin.org)
  • 69.9 v/s 55.4 mmol/L). Histological examination of renal tissue of Catha edulis treated animals revealed degenerative changes and hypercellularity in the glomeruli as well as interstitial inflammatory cell infiltration. (ajol.info)
  • How the organisms elicit this chronic inflammatory process is thought to involve the release of virulence factors, such as LPS, which are then taken up by immune cells, or through simple diffusion processes where the bacterial lipids passively slip into the host's cells. (asbmb.org)
  • Considering these facts, it could be concluded that pro-inflammatory Th17 cells may have extensive effects on LSCC pathogenesis and anti-tumor response. (spandidos-publications.com)
  • Accordingly, several experimental studies have been performed that show how the follicle functions in the resorption process that is invoked in the bone tissue during eruption. (hindawi.com)
  • So palaeontologist Mary Schweitzer of North Carolina State University attracted wide attention when she reported finding first soft tissue and later collagen from a Tyrannosaurus rex leg bone that was intact until it was broken during excavation. (com.pk)
  • Now they report recovering not just collagen - which conveys little evolutionary information because it is the same in almost all animals - but also haemoglobin, elastin and laminin, as well as cell-like structures resembling blood and bone cells. (com.pk)
  • Over time, the inflammation becomes chronic and can result in tissue damage and tooth or bone loss. (asbmb.org)
  • Our findings revealed that different genetic backgrounds of HCT116 and HT29 cell lines resulted in divergent responses to the combination treatments. (frontiersin.org)
  • Mammalian cell lines are most commonly used for recombinant antibody production, although cell lines of bacterial, yeast, or insect origin are also suitable. (cellsignal.com)
  • Western blot analysis of extracts from various tissues and cell lines using APP (E4H1U) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). (cellsignal.com)
  • We analyse two comprehensive transcriptome datasets from human tissues and human-derived cell lines in terms of the expression profiles of the SLC and ABC families of membrane transporters. (biorxiv.org)
  • The Gini index (coefficient) characterises inequalities of distributions, and is used in a novel way to describe the distribution of the expression of each transporter among the different tissues and cell lines. (biorxiv.org)
  • Similar trends hold true for the expression profiles of transporters in different cell lines, suggesting that cell lines exhibit largely similar transport behaviour to that of tissues. (biorxiv.org)
  • By contrast, the Gini coefficients for ABC transporters tend to be larger in cell lines than in tissues, implying that some kind of a selection process has taken place. (biorxiv.org)
  • The lowest four in tissues are FAM32A, ABCB7, MRPL21 and PCBP1, while the lowest three in cell lines are SF3B2, NXF1 and RBM45. (biorxiv.org)
  • Altogether there are four main datasets, namely 409 "SLCs" in 59 tissue types and 56 cell lines, and 48 ABCs in the same tissue types and cell lines. (biorxiv.org)
  • In time series experiments, which for many experimental systems are confined to laboratory cell culture experiments (cell lines), each slide corresponds to a measured time point. (lu.se)
  • and discovered a cytopathogenic effect in one of the tissue culture cell lines. (cdc.gov)
  • The novel and later successful movie franchise " Jurassic Park " popularized the idea that amber could preserve soft tissue and even DNA-molecules over millions of years. (forbes.com)
  • This simplified approach towards understanding the essence of the mechanisms, underlying the processes determining life and death of a cell has undoubtedly provided scientists with enormous amount of knowledge. (intechopen.com)
  • Using advanced equipment, scientists extract the last meal of the blood-sucking insect. (forbes.com)
  • La Jolla, CA - Salk Institute scientists have isolated cells from the brains of human cadavers that can grow, divide and form specialized classes of brain cells. (salk.edu)
  • To make it, scientists extracted cells from a part of the child's body that wasn't blistered. (technologyreview.com)
  • Using QCANet, we were able to extract several quantitative criteria of embryogenesis from 11 early mouse embryos. (nature.com)
  • This study contributes to the development of fundamental approaches for assessing embryogenesis on the basis of extracted quantitative criteria. (nature.com)
  • To quantify the nuclear region of every cell from a time-series 3D fluorescence microscopic image of living cells, we developed QCANet, a convolutional neural network-based segmentation algorithm for 3D fluorescence bioimages. (nature.com)
  • Dynamic cytoskeletal remodeling is critical to various morphogenetic and pathologic events, such as gastrulation, neural tube formation, tissue fibrosis, and tumor progression. (aacrjournals.org)
  • The results revealed that the prevalence of Th17 cells in patients with LSCC was elevated in their primary tumors, as well as in peripheral blood, compared with that in healthy controls. (spandidos-publications.com)
  • The present study revealed that patients with LSCC have elevated levels of Th17 cells in their primary tumors and peripheral blood compared with those in healthy controls. (spandidos-publications.com)
  • A total of 70 tumors and 70 adjacent control tissues (pathologically confirmed normal mucosa) were obtained from patients with LSCC undergoing surgery. (spandidos-publications.com)
  • diagnostic prediction of small round blue cell tumors (SRBCT) of childhood and determining the estrogen receptor (ER) status of sporadic breast cancer. (lu.se)
  • Initially he was examining lipopolysaccharide, a common cell wall product produced by all gram-negative organisms. (asbmb.org)
  • When LPS is expressed on the surface of the cells in the gut, it helps to protect the organisms from the bile salts, dissolution and destruction processes," Nichols says. (asbmb.org)
  • C. Peterson, M. Ringne´r / Artificial Intelligence in Medicine 28 (2003) 59-74 structure and function, cellular metabolism, development of cells and tissues, and response of organisms to their environments. (lu.se)
  • The term applies not only to entire organisms but also to copies of molecules (such as DNA) and cells. (who.int)
  • Murine skin melanoma cells (B16F0 and B16F1) also show the expression regulation of Tβ4 by Smad and TCF/LEF. (aacrjournals.org)
  • Most of the industry is focused on animal products such as meat, milk, and eggs, produced in cell culture rather than raising and slaughtering farmed livestock which is associated with substantial global problems of detrimental environmental impacts (e.g. of meat production), animal welfare, food security and human health. (wikipedia.org)
  • A West Nile virus (WNV) control was prepared by extracting RNA from a 100-L volume containing ten 50% tissue culture infective doses of WNV stock virus. (cdc.gov)
  • Thus, there remains considerable interest in the measurement of intracellular cAMP concentrations in tissues and cell culture. (enzolifesciences.com)
  • Normally metastasis is studied in a petri dish cell culture or in whole animals. (utoronto.ca)
  • These factors include FGF-2 (fibroblast growth factor 2) and cystatin C, which were added to the culture medium that bathed the cells. (salk.edu)
  • In the ThinPrep method, the number of cells on the glass slide is reduced by the presence of inflammation, blood, and mucus. (frontiersin.org)
  • Interleukin (IL)17-producing Th17 cells, which are different from Th1 and Th2 cells, have been described as serving critical roles in inflammation and autoimmune diseases, as well as in cancer development ( 8 - 11 ). (spandidos-publications.com)
  • Early polio research included the use of embryonic tissue . (salon.com)
  • The fact that the DNA of a fully differentiated (adult) cell could be stimulated to revert to a condition comparable to that of a newly fertilized egg and to repeat the process of embryonic development demonstrates that all the genes in differentiated cells retain their functional capacity, although only a few are active. (who.int)
  • BEs increased cell viability and anti-oxidant capacity, presumably by activating (phosphorylating) Akt/STAT3 and inducing MnSOD/catalase. (nih.gov)
  • The investigators obtained postmortem or biopsy tissue from 23 individuals, ranging in age from 11 weeks to 72 years old. (salk.edu)
  • The study employed postmortem and biopsy tissue from individuals with a number of neurological disorders including epilepsy and myofibromatosis. (salk.edu)
  • Oral tissue biopsy may be necessary for lesions that cannot be diagnosed on the basis of the history and clinical findings alone. (medscape.com)
  • Biopsy is often the definitive procedure that provides tissue for microscopic analysis when additional information is required to guide any indicated therapy. (medscape.com)
  • In clinical applications, which is of most relevance here, each slide corresponds to a tissue or blood sample, e.g. a biopsy. (lu.se)
  • However, RNA extracted from fresh-frozen (FF) bulk tissue without macrodissection is widely used for research purposes, and yields high-quality RNA for downstream. (lu.se)
  • The vegetables are frozen after a few hours, since the cells have been put under stress. (lu.se)
  • Other vegetables, such as peas, have a different cell structure and are less affected by being frozen altogether. (lu.se)
  • Malignancy is suspected when persistent oral mucosal lesions are red or red and white or when they are ulcerated, indurated, or fixed to deeper tissues. (medscape.com)
  • IHC: Human breast carcinoma tissue. (abcam.com)
  • Tooth eruption has been examined in both animal and human tissues. (hindawi.com)
  • This is not possible in human tissues. (hindawi.com)
  • A group of researchers from the University of Toronto has developed a credit-card sized tool for growing cancer cells outside the human body, which they believe will enhance their understanding of breast cancer metastasis. (utoronto.ca)
  • The device, described in a paper published on July 15 in Science Advances , reproduces various environments within the human body where breast cancer cells live. (utoronto.ca)
  • The study was done in collaboration with Phillip Schwartz and Stuart Stein at Children's Hospital of Orange County, Calif. The study, titled "Progenitor cells grown from postmortem human brain," was supported by the National Institutes of Health, the National Institute of Aging, the Lookout Fund and the Christopher Reeves Paralysis Foundation. (salk.edu)
  • The extract reduces the levels of two enzymes, which promote cell growth and migration of tissue growth in triple negative breast cancer. (medindia.net)
  • Studying the cells as they go through the process of invasion and metastasis could point the way toward new biomarkers and drugs to diagnose and treat cancer. (utoronto.ca)
  • If cancer cells would simply stay in one spot, it would be 'easy' to excise them and cure the disease. (utoronto.ca)
  • But when cancer metastasizes, cancer cells move through the body, making the disease difficult to treat. (utoronto.ca)
  • We decided to apply our expertise in microfluidics to develop a new tool to aid in studying how cancer cells begin to invade into surrounding tissues in the first steps in metastasis. (utoronto.ca)
  • In the paper, the researchers describe how they used a collagen matrix coated with a layer of basal membrane extract to mimic the structure of the breast tissue seen by breast cancer cells during the first step of metastasis. (utoronto.ca)
  • By placing cancer cells outside of these tissue mimics, researchers could observe the invasion process in detail, including measurements of speed and location. (utoronto.ca)
  • One interesting thing we observed is that not all cancer cells within the same population have the same invasiveness," Li said. (utoronto.ca)
  • Li and her team extracted cancer cells at various distances from the invasion point and subjected these cells to genetic sequencing. (utoronto.ca)
  • This year the FDA approved two pioneering treatments, Kymriah and Yescarta , that use a patient's own immune cells to fight rare types of cancer. (technologyreview.com)
  • If such interaction patterns can be measured for various kinds of tissues and the corresponding data can be interpreted, potential clinical benefits are obvious and novel tools for diagnostics, identification of candidate drug targets, and predictions of drug effectiveness for e.g. cancer diseases will emerge. (lu.se)
  • Transcriptomic profiling of T-cell populations in non-muscle invasive and muscle invasive bladder cancer. (lu.se)
  • Therefore, experiments were carried out on guinea pig left atrium and on pituitary GH3 cells in order to evaluate the effect of AqEx on the cellular calcium influx. (scielo.br)
  • Plasma electrolytes (Na+, K+, Ca2+ and Cl-), urea, creatine and glucose levels were investigated in eight control and fourteen experimental small East African male goats before and after treatment with Catha edulis leaf extracts. (ajol.info)
  • The polymerase chain reaction (or PCR) is widely used to replicate small fragments of DNA, but the researchers discovered that this method is not very effective with DNA extracted from resinous materials. (forbes.com)
  • Extracting and characterizing these lipids is highly specialized work, Nichols explains, and the collaboration helped build a collection of lipids that he has since shared with other researchers. (asbmb.org)
  • After cloning the antibody genes into an expression vector, this is then transfected into an appropriate host cell line for antibody expression. (cellsignal.com)
  • Virus-infected cells and cell extracts were used to develop an antibody assay. (cdc.gov)
  • But real attempts to extract DNA from amber or similar substances were unsuccessful to this day, and resin-embedded samples were deemed unsuitable for genetic examinations. (forbes.com)
  • The precise B cell subsets, mechanisms, and factors that facilitate the maintenance of latency and prevent reactivation are not fully understood. (nature.com)
  • A robust and accurate algorithm to acquire the 3D positions of the cells would help to reveal the mechanisms of embryogenesis. (nature.com)
  • Post-hypoxia, Acalypha indica Linn root extract was added at doses of 10, 15, and 20 mg/mL to 3 treatment groups. (ui.ac.id)
  • It was previously showed that aqueous leaf extract (AqEx) of Averrhoa carambola depresses the guinea pig atrial inotropism. (scielo.br)