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  • vitro
  • In-vitro colony forming cell (CFC) assays using normal human bone marrow grown in appropriate semi-solid media such as ColonyGEL have been shown to be useful in predicting the level of clinical myelotoxicity a certain compound might cause if administered to humans. (wikipedia.org)
  • These predictive in-vitro assays reveal effects the administered compounds have on the bone marrow progenitor cells that produce the various mature cells in the blood and can be used to test the effects of single drugs or the effects of drugs administered in combination with others. (wikipedia.org)
  • Mice
  • We found that MV-infected mice were more susceptible to infection with Listeria and that this corresponded with significantly decreased numbers of macrophages and neutrophils in the spleen and substantial defects in IFN-γ production by CD4+ T cells. (jci.org)
  • citation needed] G-CSF is used clinically (see Neutropenia) but tests in mice suggest it may lead to bone loss. (wikipedia.org)
  • precursors
  • Parvovirus B19 inhibits erythropoiesis by lytically infecting RBC precursors in the bone marrow and is associated with a number of different diseases ranging from benign to severe. (wikipedia.org)
  • patients
  • In immunocompromised patients, B19 infection may persist for months, leading to chronic anemia with B19 viremia due to chronic marrow suppression. (wikipedia.org)
  • mice
  • B and T cells are derived from hematopoietic stem cells, which reside in the bone marrow of adult mice and the liver of embryos. (pnas.org)
  • In particular, the bone marrow is the major site of B cell development in adult mice. (pnas.org)
  • B and D: Lung cells from SPC-KO mice transplanted with non-VSELs or HSPC express cytokeratin but not SPC. (nih.gov)
  • Biodistribution, long-term survival, and safety of human adipose tissue-derived mesenchymal stem cells transplanted in nude mice by high sensitivity non-invasive bioluminescence imaging," Stem Cells and Development , vol. 17, no. 5, pp. 993-1003, 2008. (hindawi.com)
  • Bone marrow and HSC from green fluorescent protein (GFP) transgenic mice were transplanted into irradiated wild‐type mice. (pubmedcentralcanada.ca)
  • At 1 month after transplantation, mice were sacrificed and their sclera examined by histology, immunohistochemistry (CD11b, CD11c, CD45), and transmission and scanning electron microscopy. (pubmedcentralcanada.ca)
  • To investigate bone marrow derived cell recruitment under inflammatory conditions, experimental autoimmune uveitis (EAU) was induced in transplanted mice. (pubmedcentralcanada.ca)
  • In EAU mice, vast infiltration of GFP (+) cells developed into the sclera. (pubmedcentralcanada.ca)
  • Given the close relation between hematopoietic progenitor cells and EPC, we investigated the role of CXCR4 and its downstream signaling cascade for the neovascularization capacity of cultured human EPC as well as progenitor cells derived from mice. (ahajournals.org)
  • The glycoengineered cells exhibited enhanced tethering and rolling interactions on E-selectin-bearing endothelium under flow conditions in vitro as well as increased BM trafficking and extravasation when transplanted into mice. (jci.org)
  • citation needed] G-CSF is used clinically (see Neutropenia) but tests in mice suggest it may lead to bone loss. (wikipedia.org)
  • A study in mice has shown that G-CSF may decrease bone mineral density. (wikipedia.org)
  • The gene encoding stem cell factor (SCF) is found on the Sl locus in mice and on chromosome 12q22-12q24 in humans. (wikipedia.org)
  • Mice with SCF or c-Kit mutations have severe defects in the production of mast cells, having less than 1% of the normal levels of mast cells. (wikipedia.org)
  • Unlike ES and iPS cells, transplanted Muse cells in testes of immunodeficient mice -a commonly used experiment to test the tumorigenicity of stem cells- have not been reported to form teratomas, even after six months. (wikipedia.org)
  • Human immune cells are used to develop human lymphoid organs within these immunodeficient mice, and many different types of SCID mouse models have been developed. (wikipedia.org)
  • Mice with SCIDs have lymphocyte progenitors, but these cells are unable to survive to maturity. (wikipedia.org)
  • Some SCID mice are able to produce monocytes, granulocytes, and red blood cells from the hematopoietic stem cells (HSC) present in their bone marrow. (wikipedia.org)
  • Some examples include SCID-hu Thy/Liv mice, which are given human fetal thymus and liver cells, hu-SRC-SCID mice, which are implanted with human hematopoietic stem cells (HSC), and hu-PBL-SCID mice, in which human peripheral blood mononuclear cells have been injected. (wikipedia.org)
  • In particular, it has been observed that SCID mice with an added mutation for interleukin-2 receptor common gamma chain (IL2Rγ) are better able to accept transplantation of human HSC and create human B and T cells. (wikipedia.org)
  • have found that non-obese diabetic (NOD) SCID IL2Rγ mice are even better suited as models for tissue transplants from non-self organisms due to their lower rate of rejection of human cells. (wikipedia.org)
  • Due to their immunodeficient state, SCID mice are able to accept human hematopoietic stem cells harvested from human bone marrow or thymus. (wikipedia.org)
  • This can lead to the development of human adaptive immune cells, such as B and T lymphocytes, within SCID mice, and for subsequent study of human cells in vivo. (wikipedia.org)
  • SCID mice with human immune cells are able to respond to pathogens such as viruses and create antibodies against them, which has helped scientists better understand how the human immune system protects against pathogen infection. (wikipedia.org)
  • Some studies have suggested that after a period of time, human T cells in the immunocompromised mice become anergic, meaning that they no longer respond to stimuli. (wikipedia.org)
  • It acts by binding to and activating FLT3 (CD135) which is found on what (in mice) are called multipotent progenitor (MPP) and common lymphoid progenitor (CLP) cells. (wikipedia.org)
  • NSG branded mice lack mature T cells, B cells, and natural killer (NK) cells. (wikipedia.org)
  • Mice homozygous for the mutation have severely reduced numbers of mature T and B cells. (wikipedia.org)
  • proliferation
  • By suppressing the unregulated proliferation of T and B cells, MSCs can potentially minimize and control on-going damage to the CNS. (wikipedia.org)
  • This can be explained in part by their intrinsically low telomerase activity, eradicating the risk of tumorigenesis through unbridled cell proliferation. (wikipedia.org)
  • Its role in control of stem cell proliferation has now been demonstrated for several cell types including haematopoietic, neural, and mammary stem cells. (wikipedia.org)
  • When compared to other similar chemicals, UM171 allowed for more HSC proliferation and lower apoptotic cell number compared to controls, along with a higher number in multipotential progenitors like CFU-GEMM. (wikipedia.org)
  • tissue
  • Cells present in processed lipoaspirate tissue are used to treat patients. (google.es)
  • Methods of treating patients include processing adipose tissue to deliver a concentrated amount of stem cells obtained from the adipose tissue to a patient. (google.es)
  • Compositions that are administered to a patient include a mixture of adipose tissue and stem cells so that the composition has a higher concentration of stem cells than when the adipose tissue was removed from the patient. (google.es)
  • mixing said concentrated cell population that comprises adipose-derived stem cells with a second portion of unprocessed adipose tissue from said patient. (google.es)
  • 4. The method of claim 1 , further comprising a disaggregation step, wherein said cell population that comprises adipose-derived stem cells contained in said removed first portion of adipose tissue is mechanically or enzymatically disaggregated from said mature adipocytes and connective tissue present in said first portion of adipose tissue that was removed from said patient prior to separation. (google.es)
  • Therapeutic regeneration of molecularly engineered and transplanted autologous stem cells in neurological and cardiovascular diseases due to inability of self-regeneration or repair of endogenous stem cells at sites of inflammation or disease at various stages of progress for regeneration of the damaged tissue depending upon the sites and nature of degeneration. (hoajonline.com)
  • Preimplantantion genetic diagnosis for HLA testing has been proposed as a superior method for creating a tissue matched child that can donate stem cells to its sick sibling. (bmj.com)
  • An approachable human adult stem cell source for hard-tissue engineering. (semanticscholar.org)
  • The CD34 protein is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early hematopoietic and vascular-associated tissue. (wikipedia.org)
  • Our WP currently comprises mostly of medical professionals including haemato-oncologists, infectious disease experts, doctors, nurses and scientists working in the area of cell and tissue regeneration. (isbtweb.org)
  • Since they are derived from the mesoderm, they have been shown to differentiate into bone, cartilage, muscle, and adipose tissue. (wikipedia.org)
  • As a result, many researchers have focused on adult stem cells , or stem cells isolated from adult humans that can be transplanted into damaged tissue. (wikipedia.org)
  • The hematopoietic tissue contains cells with long-term and short-term regeneration capacities and committed multipotent, oligopotent, and unipotent progenitors. (wikipedia.org)
  • Adult mesenchymal stem cells (MSCs) are being used by researchers in the fields of regenerative medicine and tissue engineering, to artificially reconstruct human tissue which has been previously damaged. (wikipedia.org)
  • Muse cells are shown to home into the damage site and spontaneously differentiate into tissue-compatible cells according to the microenvironment to contribute to tissue regeneration when infused into the blood stream. (wikipedia.org)
  • Adipose tissue (lipid cells), which requires extraction by liposuction. (wikipedia.org)
  • In practice, stem cells are identified by whether they can regenerate tissue. (wikipedia.org)
  • The virus infection provokes an inflammatory response of paneth cells in the intestine, helping to spread the virus by causing tissue damage. (wikipedia.org)
  • These cells then integrate into the site of injury, replacing damaged tissue, and thus facilitate improved function of the organ or tissue. (wikipedia.org)
  • Adipose tissue is a rich source of MSCs (or adipose-derived mesenchymal stem cells, AdMSCs). (wikipedia.org)
  • platelets
  • This condition can rapidly lead to life-threatening infection, as the body cannot produce leukocytes in response to invading bacteria and viruses, as well as leading to anaemia due to a lack of red blood cells and spontaneous severe bleeding due to deficiency of platelets. (wikipedia.org)
  • These marrow components can be divided into plasma, red blood cells, platelets, and nucleated cells. (wikipedia.org)
  • The cell ceases its growth at 4N, 8N or 16N, becomes granular, and begins to produce platelets. (wikipedia.org)
  • After budding off platelets, what remains is mainly the cell nucleus. (wikipedia.org)
  • Because of the failure of hematologic components to develop-white blood cells, red blood cells, and platelets-the body's capabilities to fight infection, deliver oxygen, and form clots are all diminished. (wikipedia.org)
  • Symptoms caused by bone marrow damage Bruising, spotting: the reason is lack of platelets, it is very common in BAL patients, most of patients die due to the Anemia: Because the decline of hematopoietic function, need blood transfusion therapy Persistent fever, infection prolonged healing: Diffuse hemorrhage: also called Septicemia, which is dangerous and might lead to death. (wikipedia.org)
  • The CFU-GEMM cell is capable of differentiating into white blood cells, red blood cells, and platelets, all of which are normally found in circulating blood. (wikipedia.org)
  • bloodstream
  • Homing of HSPCs from bloodstream to bone marrow (BM) is an important aspect of HSPC biology that has remained unaddressed in efforts to derive functional HSPCs from human PSCs. (jci.org)
  • The decrease in blood cell counts does not occur right at the start of chemotherapy because the drugs do not destroy the cells already in the bloodstream (these are not dividing rapidly). (wikipedia.org)
  • Granulocyte-colony stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein that stimulates the bone marrow to produce granulocytes and stem cells and release them into the bloodstream. (wikipedia.org)
  • neural
  • This complicates the design of drugs, for instance neural stem cell targeted therapies for the treatment of clinical depression. (wikipedia.org)
  • therapeutic
  • Cancer stem cellsand their susceptibility over their expected to be "downstream or progeny" cancer cells represent a target for therapeutic interventions to prevent latency and consequently relapse or metastasis of the malignant disease. (hoajonline.com)
  • Autologous hematopoietic stem cell transplantation and reconstitution: Different species and including primates and humans for studies are being used for development, localization, and therapeutic strategies. (hoajonline.com)
  • For several lethal malignant disorders and also for some non-malignant disorders bone marrow or blood cell transplantations are currently the only therapeutic approach. (bmj.com)
  • Therapeutic window for treatment of cortical ischemia with bone marrow-derived cells in rats," Brain Research , vol. 1306, pp. 149-158, 2010. (hindawi.com)
  • We believe this approach, which is backed by our extensive intellectual property, is broadly applicable to the development of hematopoietic cell therapies with novel biological properties and enhanced therapeutic functionality. (cnbc.com)
  • Importantly, these newly patented compositions and methods are independent of the specific modulators used to enhance the cells' biological properties and therapeutic function. (cnbc.com)
  • Transfusion experts, manufactures of Human Platelet Lysate, scientist who are investigating its therapeutic and cell culture potential, as well as regulators have met and discussed the latest updates in the field. (isbtweb.org)
  • 1-3 Increasing evidence suggests that transplantation of culture-expanded progenitor cells or bone marrow-derived progenitor cells successfully promotes therapeutic neovascularization in both ischemic hindlimbs as well as acute myocardial infarction models. (ahajournals.org)
  • 8-11 Further evidence indicates that not only the cell number but also functional properties of transplanted cells determine the therapeutic success in autologous stem cell transplantation. (ahajournals.org)
  • To help discover the therapeutic uses of these stem cells they are grown in laboratories or by using medication to stimulate new cell growth within the human body. (wikipedia.org)
  • ESCs possess many of the same therapeutic properties as MSCs, including the ability to self-regenerate and differentiate into a number of cell lineages. (wikipedia.org)
  • The exact therapeutic mechanisms of MSCs in the treatment of MS are still very much up to debate among stem cell researchers. (wikipedia.org)
  • There is yet no consensus among biologists on the prevalence and physiological and therapeutic relevance of stem cell plasticity. (wikipedia.org)
  • Nevertheless, these cells are being investigated as the basis for a number of therapeutic applications, including possible treatments for diabetes and Parkinson's disease. (wikipedia.org)
  • This includes cells that naturally secrete the relevant therapeutic factors, or which undergo epigenetic changes or genetic engineering that causes the cells to release large quantities of a specific molecule. (wikipedia.org)
  • Regenerative
  • Regenerative medicine has advanced two major stem cell-based therapies for CNS disorders, namely, transplantation of exogenous stem cells and amplification of endogenous neurogenesis. (cognizantcommunication.com)
  • These regenerative endodontic echniques will possibly involve some combination of disnfection or debridement of infected root canal systems ith apical enlargement to permit revascularization and se of adult stem cells, scaffolds, and growth factors. (semanticscholar.org)
  • Marrow nucleated cells are used every day in regenerative orthopedics. (wikipedia.org)
  • In 2006 Eaves retired as required by provincial law at that time, becoming Professor Emeritus of Hematology and spending more time on several companies he founded to further the fields of cellular therapy and regenerative medicine - STEMCELL Technologies, STEMSOFT Software Inc. and Malachite Management Inc. These companies continue to grow under his leadership and by 2016, STEMCELL had over 900 employees making Canada's largest biotechnology company. (wikipedia.org)
  • Several different forms of stem cells have been identified and studied in the field of regenerative medicine. (wikipedia.org)
  • Therefore, an ideal treatment must possess both immunomodulating properties to control irregular autoimmune responses to prevent further damage and regenerative properties to stimulate natural repair mechanisms and replace damaged cells. (wikipedia.org)
  • gene
  • IL2RG, RAG1 and/or RAG2 gene) into the genome of a cell for provision of proteins lacking or deficient in SCID. (patents.com)
  • Mast/stem cell growth factor receptor (SCFR), also known as proto-oncogene c-Kit or tyrosine-protein kinase Kit or CD117, is a receptor tyrosine kinase protein that in humans is encoded by the KIT gene. (wikipedia.org)
  • BCR/ABL induce cell adhesive and migratory abnormalities because the mutation will lead an abnormal response to chemokine SDF-1 MLL gene encode Histone-lysine N-methyltransferase (HRX), which is a histone methyltransferase. (wikipedia.org)
  • The NOD/ShiLtJ background also contributes an allele of the Sirpa gene that renders the bone marrow niche very permissive to colonization by human hematopoietic stem cells. (wikipedia.org)
  • blood
  • Blood cell production is a tightly regulated process that, after birth and throughout life, starts in a conspicuous hematopoietic stem cell (HSC) subset residing within the bone marrow (BM). (hindawi.com)
  • Following this preparative regimen, you will receive the stem cells intravenously (similar to a blood transfusion). (cancercenter.com)
  • CD34+ cells may be isolated from blood samples using immunomagnetic or immunofluorescent methods. (wikipedia.org)
  • Bone marrow suppression also known as myelotoxicity or myelosuppression, is the decrease in production of cells responsible for providing immunity (leukocytes), carrying oxygen (erythrocytes), and/or those responsible for normal blood clotting (thrombocytes). (wikipedia.org)
  • Instead, the drugs affect new blood cells that are being made by the bone marrow. (wikipedia.org)
  • Because the bone marrow is the manufacturing center of blood cells, the suppression of bone marrow activity causes a deficiency of blood cells. (wikipedia.org)
  • CD117 is an important cell surface marker used to identify certain types of hematopoietic (blood) progenitors in the bone marrow. (wikipedia.org)
  • Hematopoietic progenitor cells are normally present in the blood at low levels. (wikipedia.org)
  • G-CSF stimulates the production of granulocytes, a type of white blood cell. (wikipedia.org)
  • Most of these cells are CD34+ heme progenitors (destined to differentiate into blood components), while very few are actually MSC's capable of differentiating into bone, cartilage, or muscle. (wikipedia.org)
  • It must balance enormous production needs (more than 500 billion blood cells are produced every day) with the need to precisely regulate the number of each blood cell type in the circulation. (wikipedia.org)
  • Using the ratio ρ = L / M {\displaystyle \rho =L/M} of lymphoid (L) to myeloid (M) cells in blood as a quantitative marker, the stem cell compartment can be split into three categories of HSC. (wikipedia.org)
  • In recognition of his research accomplishments and leadership in moving basic science discoveries in stem cell biology into the clinic, he was elected President of the International Society of Cellular Therapy (1995-1997), Treasurer of the Foundation for the Accreditation of Cellular Therapy (1995-2002) and President of the American Society of Blood and Marrow Transplantation (1999-2000). (wikipedia.org)
  • In general, megakaryocytes are 10 to 15 times larger than a typical red blood cell, averaging 50-100 μm in diameter. (wikipedia.org)
  • These multipotent stem cells live in the marrow sinusoids and are capable of producing all types of blood cells depending on the signals they receive. (wikipedia.org)
  • Alternatively, the cell may form platelet ribbons into blood vessels. (wikipedia.org)
  • This crosses the bone marrow barrier to the blood and is consumed in the lung by alveolar macrophages. (wikipedia.org)
  • By definition, autologous cells are obtained from one's own body, just as one may bank his or her own blood for elective surgical procedures. (wikipedia.org)
  • This demonstrates that the cells can produce new blood cells over a long term. (wikipedia.org)
  • Among those affected, the majority develops cancer, most often acute myelogenous leukemia, and 90% develop bone marrow failure (the inability to produce blood cells) by age 40. (wikipedia.org)
  • Because macrocytosis usually precedes a low platelet count, patients with typical congenital anomalies associated with FA should be evaluated for an elevated red blood cell mean corpuscular volume. (wikipedia.org)
  • While at birth, blood count is usually normal, macrocytosis/megaloblastic anemia, defined as unusually large red blood cells, is the first detected abnormality, often within the first decade of life (median age of onset is 7 years). (wikipedia.org)
  • Within the next 10 years, over 50% of patients presenting haematological abnormalities will have developed pancytopenia, defined as abnormalities in two or more blood cell lineages. (wikipedia.org)
  • Early studies used simple models and only considered the cell-free spreading of HIV, in which virus particles bud from an infected T cell, enter the blood/extracellular fluid, and then infect another T cell. (wikipedia.org)
  • Anemia: reduction metrocytes that could produce red blood cells, resulting in a lack of red blood cells. (wikipedia.org)
  • Persistent fever, infection prolonged healing: Most of the white blood cells are leukemia cells, no normal function, leading to decreased immunity, susceptible to infection. (wikipedia.org)
  • Following observation of the symptoms, the patients need to get complete blood counts and a bone marrow examination. (wikipedia.org)
  • When erythropoietin (EPO) is present, red blood cell production from the CFU-GEMM will be activated. (wikipedia.org)
  • Since the CFU-GEMM cell is a very early ancestor of the mature cells of the blood, it is not normally found in the blood. (wikipedia.org)