Cells surface markers immunomagnetic. Medical search. Frequent questions

The authors propose a new strategy that allows isolation of CD44 + /CD24 − tumor initiating cells (TICs) by immunomagnetic separation involving both magnetic beads coated by anti-CD44 antibody and nonmagnetic beads coated by anti-CD24 antibody. (stemcellsciencenews.com)
We screened a collection of 312 annotated antibodies and uncovered integrin-associated protein (IAP, Compact disc47) being a cell surface area marker ideal for immunomagnetic isolation of FOXA2+ hPSC-derived mesDA progenitor cells with flooring plate identity. (epf2013.org)
For these good reasons, an alternative solution or additional technique to detect and/or monitor FR appearance is certainly through the isolation and evaluation of circulating tumor cells (CTCs) from peripheral bloodstream. (healthanddietblog.info)
isolation exploits the distinctions in intrinsic dielectric properties between cancers cells and regular bloodstream cells.44 Information about the DEP frequency are described in the scholarly research by Gupta et al.44, in short, 65 kHz was herein employed for the samples reported. (healthanddietblog.info)
Single-cell sequencing of microbial genomes entails technical challenges relating to the various steps of the required workflow: isolation of individual cells, whole-genome amplification, DNA sequencing, and sequence analysis (Fig. 1 ). (biomedcentral.com)
These methods permit targeted isolation of a cell from mixed populations in liquid medium, but isolating microbial cells from primary samples such as swabs and biopsies remains challenging, especially from solid tissues requiring homogenization. (biomedcentral.com)
Experimental steps include ( upper half ) isolation and lysis of single cells with subsequent amplification of their genomes, followed by ( lower half ) high-throughput sequencing, genome assembly and genome analysis. (biomedcentral.com)
Stiefel's thesis deals with detecting tumor features based on the automated isolation of circulating tumor cells from liquid biopsies and thus contributes significantly to establishing a platform technology of Fraunhofer IMM in applied research of liquid biopsy. (fraunhofer.de)
The defense of his dissertation on "Automated Isolation of Circulating Tumor Cells and their Molecular Biological Analysis" took place on January 30, 2023 at the Johannes Gutenberg University Mainz. (fraunhofer.de)
Against this background, the Fraunhofer Institute for Microengineering and Microsystems has recognized the unmet need of standardized CTC isolation and developed automated microfluidic platforms to enrich, detect and isolate single tumor cells from 7.5 mL whole blood. (fraunhofer.de)
The aim of this thesis was to realize single cell isolation by means of automated immunomagnetic bead enrichment and antibody-based fluorescence detection in a chip-based microfluidic system and the molecular biological characterization of these cells to assay tumor features from liquid biopsy. (fraunhofer.de)
In a prototype enrichment device (IsoMAG), we established an isolation protocol with EpCAM-targeting 1 µm magnetic beads for automated enrichment tested on different epithelial cancer cell lines. (fraunhofer.de)
40 to 56.7 percent of cells were recovered after complete isolation from 7.5 mL untreated whole blood. (fraunhofer.de)
We highlighted the bottlenecks of microfluidic cell isolation in dependency of bead-binding on the cell surface and implemented a gating function for the cytometer subunit to selectively dispense cells instead of autofluorescent objects. (fraunhofer.de)
Materials and Methods Isolation tradition and illness of primary human being CD34+ cells and of U937 cells The human being hematopoietic cell collection U937 was cultured in RPMI-1640 (Existence Systems Carlsbad CA USA) comprising 10% fetal bovine serum (FBS) (Existence Technologies) inside a humidified incubator at 37°C and 5% CO2. (acancerjourney.info)
Plasma-derived EVs were captured and detected using this straightforward protocol, sequentially combining isolation and staining of specific surface markers, such as CD9 or CD41. (biomedcentral.com)
In this review article we aimed to emphasize the importance of single-cell analyses by presenting tumor heterogeneity and the limitations of conventional investigational approaches, followed by an overview of the whole single-cell analytic workflow from sample isolation to amplification, sequencing and bioinformatic analysis and a review of recent literature regarding the single-cell analysis of colorectal cancers. (por-journal.com)
In this review article, we aimed to present contemporary methods and techniques for the sampling, isolation, and analysis of single cells and to give an overview of the current scientific literature about CRC at the single-cell level. (por-journal.com)
Morphological-based isolation separates CTCs according to size discrepancies, using isolation by size of epithelial tumor cells (ISET) or according to density, using density-gradient separation ( 4 ). (iiarjournals.org)
Immunological techniques, which are the most widely used methods, employ immunomagnetic isolation ( 4 ). (iiarjournals.org)
The heterogeneous expression of markers, together with their rare frequency (1-10 CTCs in 8 mL of blood) are the main intrinsic factors hampering CTC isolation and limiting in-depth study of these cells in patients with melanoma. (nature.com)

Right here, we demonstrate that integrin-associated protein (IAP) is really a cell surface area marker ideal for enrichment of hPSC-derived mesDA progenitor cells. (epf2013.org)
Next, we optimized post-HITI CRISPR EnrichMENT (CEMENT) to seamlessly integrate it into a 14-day process and compared our knock-in with viral transduced anti-GD2 CAR-T cells. (biomedcentral.com)
CTC enrichment, isolated cells could be enumerated, interrogated, and seen as a immunofluorescence recognition of biomarkers using laser beam checking cytometry (LSC) quantitative evaluation. (healthanddietblog.info)
enrichment, to recognize FR+ CTCs from bloodstream examples collected from sufferers with four types of metastatic cancers: NSCLC adenocarcinoma, breasts cancer, ovarian cancers, and squamous cell lung cancers. (healthanddietblog.info)
The field of circulating tumor cell (CTC) enrichment has seen many emerging technologies in recent years, which have resulted in the identification and monitoring of clinically relevant, CTC-based biomarkers that can be analyzed routinely without invasive procedures. (oaepublish.com)
Therefore, this work compares manual and automated immunomagnetic enrichment in terms of cell recovery in dependency of cell line and input number, bead characteristics, biofunctionaliza-tion protocol and blood cell contamination focusing on head and neck squamous cell carcinoma (HNSCC) and metastatic breast cancer. (fraunhofer.de)
In the microfluidic benchtop platform CTCelect, we characterized each process step with two different carcinoma cell lines (MCF-7, SCL-1) demon-strating up to 87 Percent enrichment, 73 percent optical detection and dispensing efficiency. (fraunhofer.de)
Fluorescent EV spike recovery evidenced that target EV subpopulations can be efficiently retrieved from plasma, and that their enrichment is dependent not only on complex matrix composition, but also on the EV surface phenotype. (biomedcentral.com)

Potential subpopulations of exosomes can be captured by targeting these markers using magnetic beads. (exosome-rna.com)
CD34+ cells were isolated from umbilical wire blood (CB) from consenting donors using immunomagnetic beads (StemCell Systems Grenoble France). (acancerjourney.info)
Beads with covalently-conjugated antibodies on their surface outperformed streptavidin-conjugated beads, coated with biotinylated antibodies, in EV immunoprecipitation. (biomedcentral.com)
Anti-GFP Tag mouse monoclonal antibody at 1/500-1/2000 dilution and 60μg of Immunomagnetic beads Protein G. Goat Anti-Mouse IgG H&L (Dylight800) at 1/10000 dilution. (thenewsindependent.com)
These CD34+ cells were isolated using immunomagnetic beads (Miltenyi, #130-046-702) after gradient centrifugation (Axis-Shield PoC AS, Norway). (biomedcentral.com)

The orthotopic mouse model of 4T1 and the experimental mouse model of MDA-MB-231 cell line expressing firefly luciferase demonstrated that SH treatment inhibited breast cancer metastasis by inhibiting epithelial-mesenchymal transition and cancer stem cell properties without obvious hepatotoxicity and renal toxicity. (stemcellsciencenews.com)
The immunogen used to generate the G8.8 antibody was the TE-71 thymic epithelial cell line. (thermofisher.com)
CD326 is expressed on the majority of epithelial cells, and is considered a pan-carcinoma antigen. (thermofisher.com)
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. (thermofisher.com)
Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. (thermofisher.com)
Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. (thermofisher.com)
In addition, we studied the replication kinetics of SARS-CoV-2/SB3-TYAGNC in human fibroblast, epithelial, and immune cells. (cdc.gov)
This method uses either epithelial cell-specific markers which are generally expressed in all tumor cells, or tumor markers expressed by specific types of cancer ( 13 ). (iiarjournals.org)
The presence of high expressing epithelial cell adhesion molecule (EpCAM high ) circulating tumor cells (CTC) enumerated by CellSearch ® in blood of cancer patients is strongly associated with poor prognosis. (oncotarget.com)

The discovery of various proteins specific to certain cells led to the production of cell-type-specific antibodies that have been used to identify cells. (wikipedia.org)
The most important neural markers used nowadays are the GFAP, Nestin, NeuroD antibodies and others. (wikipedia.org)
The device, which is sandwiched by permanent magnets, balances magnetic forces and fluidic drag forces to sort cells labelled with magnetic nanoparticles conjugated with antibodies for the target markers. (northwestern.edu)

As briefly illustrated in Tables one and two, there are many combina tions of cell surface markers that can be employed to pick MSCs from mixtures of cells, that are often performed by cell sorting using FACS or immunomagnetic procedures, although several scientific studies use plastic adher ent stromal cells that are subsequently classified using immunofluorescent phenotyping or movement cytometry. (plasignaling.com)
White blood cell contamination, usually hampering further downstream analysis of enriched tumor cells, was measured by FACS and minimized to about 1200 cells starting from 7.5 mL blood. (fraunhofer.de)
Background and Aim Overexpression of (expression was measured by quantitative real time reverse transcriptase polymerase chain reaction (qRT-PCR) and intranuclear fluorescence activated cell sorting (FACS). (acancerjourney.info)
Differentiation was permitted to move forward for 7-13 times based on lineage and was supervised by FACS for lineage-specific cell surface area markers (Compact disc15 for granulocytes Compact disc14 for monocytes. (acancerjourney.info)

Our buoyancy-activated technology is an innovative alternative to traditional blood separation techniques , significantly reducing costs, saving time, and simplifying workflows-all while improving the quality and viability of sorted cells. (akadeum.com)
Red blood cell depletion microbubbles offer a gentler cell separation approach. (akadeum.com)

Investigators analyzed the effects of Resveratrol exposure on cell viability, proliferation and motility in seven glioma stem cell (GSC) lines isolated from glioblastoma multiforme patients. (stemcellsciencenews.com)
It's of curiosity to determine that MSCs also express cell surface epidermal development issue receptor one and reply to the ligand heparin binding epidermal growth element with dose dependent proliferation, which reversibly impairs their trilineage differentiation potential right up until the stimulus is removed. (plasignaling.com)
The progression of chronic lymphocytic leukemia (CLL) is strongly associated with the modification of diverse immune cell populations to create a niche appropriate for the proliferation and survival of leukemic B cells. (biomedcentral.com)
The most abundant and best characterized of these are EVI1 a 1053 amino acid protein with a well-established oncogenic function and myelodysplastic syndrome 1 (MDS1)/EVI1 which in addition to the entire GF 109203X sequence harbours an is mostly expressed in stem and progenitor cells and is necessary because of their maintenance and proliferation (7-10). (acancerjourney.info)
In summary a number of reports have shown that manifestation of causes improved proliferation and delayed differentiation of murine hematopoietic stem and progenitor cells. (acancerjourney.info)
CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. (thermofisher.com)

Compared with controls, the detectable proportion of HIV-1 particles derived from CD14 macrophages and CD26 lymphocytes was increased in persons with acute malaria coinfection and correlated with markedly increased plasma concentrations of both proinflammatory cytokines and soluble markers of macrophage and lymphocyte activation. (lww.com)
and 0.5 ng/ml tumor necrosis factor α(TNFα) (all cytokines from Peprotech) for dendritic (Langerhans cell LC) differentiation. (acancerjourney.info)
The changes of immune cells and cytokines in vitro and in vivo were detected by flow cytometry and enzyme-linked immunosorbent assay (ELISA). (biomedcentral.com)
Besides, Niraparib activated the cGAS-STING pathway, increasing the levels of cytokines such as CCL5 and CXCL10, which played a vital role in augmenting the infiltration and activation of cytotoxic T cells. (biomedcentral.com)
In our study, we investigated the dynamic changes and responses of immune cells and cytokines during Niraparib treatment. (biomedcentral.com)
They are more heterogeneous than normal fibroblasts and express various surface receptors and cytokines that facilitate tumor progression, angiogenesis, etc. [ 13 ]. (por-journal.com)
Partial interspecies cross-reactivity between murine cytokines/chemokines or MHC and co-stimulatory molecules with their human cognate receptors, might reduce the survival capacity of engrafted human cells, possibly affecting the decay of the latent reservoir. (biomedcentral.com)

The EasySep™ Human CD56 Positive Selection Kit II is designed to isolate CD56+ cells from fresh or previously frozen peripheral blood mononuclear cells and human skeletal muscle (myoblasts and fibroblasts) cultures by positive selection. (stemcell.com)
For example, human peripheral blood mononuclear cells (PBMCs) are often used for studying immunological functions like cellular surface marker expression and cytokine production. (akadeum.com)
We confirm that human primary peripheral blood mononuclear cells are not permissive for SARS-CoV-2. (cdc.gov)
To investigate the transcriptional diversity of BM-MSCs, we applied single-cell RNA sequencing (scRNA-seq) on freshly isolated CD271 + BM-derived mononuclear cells (BM-MNCs) from two human subjects. (ijbs.com)

Scientists report that sulforaphane (SFN), a potent anti-cancer and well-tolerated dietary compound, inhibited cancer stem-like cell properties and enhanced therapeutic efficacy of cisplatin in human non-small cell lung cancer. (stemcellsciencenews.com)
Compared with conventional cell sorting, immunomagnetic cell sorting recovered up to 30-fold higher numbers of TILs, and the higher levels and diversity of the recovered TILs accelerated TIL expansion and enhanced their therapeutic potency. (northwestern.edu)
Stem/progenitor cell transplantation is a potential novel therapeutic strategy to induce angiogenesis in ischemic tissue, which can prevent major amputation in patients with advanced peripheral artery disease (PAD). (bvsalud.org)
Chimeric Antigen Receptor (CAR) T cells are now standard of care (SOC) for some patients with B cell and plasma cell malignancies and could disrupt the therapeutic landscape of solid tumors. (biomedcentral.com)
Additional fully non-viral CRISPR knock-in platforms are needed to ensure adequate access to meet the increasing demand for engineered T cells for therapeutic use. (biomedcentral.com)
CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. (thermofisher.com)
The enumeration of circulating tumor cells (CTCs) represents an effective prognostic and predictive biomarker, which is able to monitor efficacy of adjuvant therapies, detect early development of (micro)metastases and at last, assess therapeutic responses of advanced disease earlier than traditional imaging methods. (amegroups.org)
Additionally, our data highlight the potential exploitation of T cell autophagy as a biomarker of disease activity and provide new ground to verify the therapeutic implications of autophagy as an innovative drug target in pSS. (biomedcentral.com)

Adoptive cell therapies require the recovery and expansion of highly potent tumour-infiltrating lymphocytes (TILs). (northwestern.edu)
CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. (thermofisher.com)
Lymphocytes are recruited to tissues from blood via binding to vessels in the target tissue mediated by interactions between specific receptors on the lymphocyte surface and ligands on the endothelium. (biomedcentral.com)
T lymphocytes were analyzed for the expression of autophagy-specific markers by biochemical, molecular, and histological assays in peripheral blood and labial gland biopsies. (biomedcentral.com)
Although the aberrant activation of the T cell response observed in pSS has partially been explained by the interaction of T lymphocytes both with the ductal epithelium and the infiltrating B cells via costimulatory molecules (i.e. (biomedcentral.com)

B) hESCs (H9) and hiPSCs (hFF-iPS) had been differentiated toward mesDA progenitor cells and screened for marker appearance on d11 and d16 of differentiation. (epf2013.org)
C) Comparative evaluation from the 12 surface area markers portrayed in hESCs and hiPSCs at d11 and d16 of differentiation. (epf2013.org)
Cyto-Chex BCT is a direct draw blood collection tube that maintains cellular morphology and surface antigen expression, including cluster of differentiation (CD) markers prior to analysis by flow cytometry. (streck.com)
We consequently set out to study the manifestation and function of in main human being cluster of differentiation 34 positive (CD34+) hematopoietic progenitor cells. (acancerjourney.info)
For megakaryocyte differentiation cells had been extended for 6 times in StemPro-34 mass media (Life Technology) filled with 1 ng/ml IL-3 1 ng/ml SCF and 50 ng/ml TPO (all from Miltenyi Biotec Bergisch Gladbach Germany) and differentiated in mass media supplemented GF 109203X with 50 ng/ml TPO. (acancerjourney.info)
We successfully identified LEPR hi CD45 low BM-MSCs within the CD271 + BM-MNC population, and further codified the BM-MSCs into distinct subpopulations corresponding to the osteogenic, chondrogenic, and adipogenic differentiation trajectories, as well as terminal-stage quiescent cells. (ijbs.com)
We also discovered transcripts for several clusters of differentiation (CD) markers that were either highly expressed (e.g. (ijbs.com)

The info confirmed that FR+ CTCs had been discovered in bloodstream from NSCLC adenocarcinoma, breasts, and ovarian cancers sufferers, whereas squamous cell lung cancers patients and regular healthy handles lacked FR+ CTCs as previously known. (healthanddietblog.info)
While FR+ CTCs had been isolated in examples from NSCLC adenocarcinoma, breasts cancers, and ovarian cancers, FR+ CTCs weren't isolated from either lung squamous cell carcinoma sufferers or healthful donors, demonstrating the specificity from the technique. (healthanddietblog.info)
Ensemble-decision aliquot position (eDAR) is a sensitive and high-throughput method to analyze circulating tumor cells (CTCs) from peripheral blood. (conferencedequebec.org)
The ultimate challenge is based on the need for downstream analyses of the isolated CTCs providing cellular and molecular information at the single-cell level which may be more important than simple enumeration. (conferencedequebec.org)
The use of circulating tumor cells (CTCs) as a "liquid biopsy" to interrogate the molecular profile of single patients is extremely encouraging, as these cells originate from primary tumor and metastatic sites, providing a comprehensive fingerprint of a given tumor at any time during the course of the disease with a simple blood draw [ 7 ] . (oaepublish.com)
Cancer metastases descend from proliferative circulating tumor cells (CTCs) which disseminate from the primary tumor via blood and lymph vessels. (fraunhofer.de)
Nonetheless the small number of CTCs compared to billions of blood cells in a patient sample requires sensitive methods to obtain reproducible tumor cell recovery rates. (fraunhofer.de)
In the last decade, the detection of disseminated tumor cells (DTCs) in the bone marrow and circulating tumor cells (CTCs) in the blood have demonstrated to provide useful information for the clinical management of BC by predicting treatment benefit earlier than traditional imaging methods. (amegroups.org)
An emerging method to detect metastasis is the analysis of circulating tumor cells (CTCs), which has shown promise in filling the gaps left by other diagnostic methods. (iiarjournals.org)
CTCs are tumor cells originating from primary sites or metastases that circulate in the patients' bloodstream and are very rarely found in healthy individuals ( 9 , 10 ) ( Figure 1 ). (iiarjournals.org)
Circulating tumour cells (CTCs) can be assessed through a minimally invasive blood sample with potential utility as a predictive, prognostic and pharmacodynamic biomarker. (nature.com)
Circulating tumour cells (CTCs), which are responsible for haematogenous spread of tumours, also reflect this heterogeneity. (nature.com)
In this study, we compared the recovery and purity of melanoma cells isolated through different microfluidic devices, Parsortix (Angle plc, Surrey, United Kingdom) and ClearCell® FX1 system (Biolidics, Singapore), which enrich CTCs based on their differential cell size, density and deformability. (nature.com)

However, most of these studies were performed on cells isolated from peripheral blood. (biomedcentral.com)
For our in vivo and ex vivo experiments, we inoculated wild-type mice with TCL1 leukemic cells isolated from Eµ-TCL1 transgenic mice and then monitored disease progression by detecting leukemic cells in peripheral blood. (biomedcentral.com)
Applications Tested: This 2H7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
Methods: Identification of tumour-associated endothelial markers (TEM) by comparing antigen expression on normal vs t-CEC and assess the presence of t-CEC in peripheral blood of cancer patients by incorporating TEM in our novel flow cytometry-based CEC detection assay. (researchgate.net)

Cells were harvested for the flow-cytometry-based surface area marker verification on d16 and d11. (epf2013.org)
Several approaches have been developed to isolate single cells by using either serial dilution, microfluidics, flow cytometry, micromanipulation, or encapsulation in droplets [ 1 ]. (biomedcentral.com)
EVs were characterized by transmission electron microscopy and fluorescence nanoparticle tracking analysis, and EV surface protein profiles were analyzed by multiplex bead-based flow cytometry to identify tumor- or immune system-related EVs of AML, ALL, CLL, and healthy samples. (nature.com)
Based on Streck's proprietary cell-stabilization technology, we offer a comprehensive line of positive procedural controls and cellular stabilization products for immunophenotyping by flow cytometry. (streck.com)
Flow cytometry allows scientists to rapidly analyze thousands of normal cells and identify the presence of rare and abnormal cells. (streck.com)
Programmed death ligand 1 (PD-L1) expression in human ovarian cancer cells after PARP inhibitors treatment was examined by western blotting (WB) and flow cytometry. (biomedcentral.com)
Cell-generated EV spike-in models were isolated and purified by size-exclusion chromatography, stained with cellular dyes and characterized by nano flow cytometry. (biomedcentral.com)

The basic conclusion is that MSCs or their closely relevant cousins can reside in or pass via most tissues, and that this kind of cells may be isolated and/or selelck kinase inhibitor cultured by standard methods this kind of as fluorescence activated cell sorting or plastic adherence, and will be shown to possess multipotency. (plasignaling.com)
Proven is the proportion from the mean fluorescence strength (MFI) for every marker for FOXA2+ and FOXA2? (epf2013.org)

Extracellular Vesicles (EVs) are membranous vesicles produced by all cells under physiological and pathological conditions. (nature.com)
Exosomes, nano-sized extracellular vesicles (EVs) secreted by almost all cell types, are found in biological fluids and central nervous system tissues. (creative-proteomics.com)
Exosomes are here defined as extracellular vesicles (EVs) in the approximate size range of 30-100 nm in diameter, and are observed in most body fluids containing typical exosomal markers such as CD9, CD63, and CD81. (exosome-rna.com)
Intercellular communication is mediated by extracellular vesicles (EVs), as they enclose selectively packaged biomolecules that can be horizontally transferred from donor to recipient cells. (biomedcentral.com)
Extracellular vesicles (EVs), including exosomes, are membranous particles released by cells into the extracellular space. (mdpi.com)

However, the specific role of innate immune cells, especially CCR2 macrophages on the outcome of cardiomyocyte transplantation, is unclear. (bvsalud.org)
Regularly, anergy of T cells or other immune cells can be regularly reverted to anti-leukemic functionality. (nature.com)
We also tested a wide range of human immune cells for productive infection with SARS-CoV-2. (cdc.gov)
Our results showed that Niraparib could upregulate PD-L1 expression, but did not impair the function of immune cells, especially CD8 + T cells. (biomedcentral.com)
VAP-1 is downregulated in human CRC and although the molecular basis of this down regulation is not yet known, we suggest it may be part of a mechanism used by the tumour to prevent the recruitment of anti-tumour immune cells. (biomedcentral.com)
Cancerous cells are embedded into diverse tissues consisting of cancer-associated fibroblasts, extracellular matrix, vascular and lymphatic networks, and immune cells, among others. (por-journal.com)

Staining of the TE-71 cell line with 0.06 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (blue histogram) or 0.06 µg of Anti-Mouse CD326 (EpCAM) APC (purple histogram). (thermofisher.com)

Depending on the goal of a particular study, one can use neural stem cells markers, neural progenitor cell markers, neuron markers or PNS neuronal markers. (wikipedia.org)
The aim of this study was to identify early diabetes-related changes in the expression of genes that have been reported to be closely involved in endothelial progenitor cell migration and function. (biomedcentral.com)
Based on review of current literature, this study examined the expression level of 35 genes that are known to be involved in endothelial progenitor cell migration and function in magnetically sorted Lin - /VEGF-R2 + endothelial progenitor cells obtained from the bone marrow of Akita mice in the early stages of diabetes (18 weeks) using RT-PCR and Western blotting. (biomedcentral.com)

Magnetic cell sorting continues to be reported to permit quicker and gentler managing of cells (Bosio et?al. (epf2013.org)
2014). Significantly, magnetic cell sorting can be employed in large-scale scientific techniques under sterile circumstances (Despres et?al. (epf2013.org)
The Exo-Flow™ magnetic antibody bead kit from System Biosciences enables the selective capture and purification of exosomes based on specific surface markers. (bioscience.co.uk)

Immunomagnetic HIV-1 capture analysis was used to determine the cellular origin of cell-free virus particles present in all 30 plasma samples and indices of immune activation were measured using enzyme-linked immunosorbent assays. (lww.com)
METHODS: We compared the cellular, molecular, and functional outcome following cardiomyocyte transplantation in wildtype and T cell- and B cell-deficient Rag2del mice. (bvsalud.org)
Immunostaining is used in cell biology to study differential protein expression, localization and distribution at the tissue, cellular, and subcellular level. (thenewsindependent.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)

Exosomes are cell-derived vesicles that are present in many and perhaps all biological fluids, including blood, urine, and cultured medium of cell cultures. (exosome-rna.com)

Several molecular platforms have been used to investigate the molecular profile of the disease, from high throughput gene expression analyses down to single cell biological dissection. (oaepublish.com)
The process of depletion is when a single cell type, such as red blood cells, is removed from a biological sample. (akadeum.com)
As a critical part of sample preparation, RBC depletion is intended to clean up red blood cell contamination in biological samples, such as in bone marrow or dissociated tissue samples. (akadeum.com)
When RBC depletion microbubbles are added to a biological sample, they engage RBCs and rapidly lift the unwanted cells to the top of any container, regardless of shape or size. (akadeum.com)
In recent years, the evolution of the molecular biological technical background led to the widespread application of single-cell sequencing, a versatile tool particularly useful in the investigation of tumor heterogeneity. (por-journal.com)

However, these methods are generally unable to reconstruct how DNA is compartmentalized into cells, which is needed to understand population structure with the cell as the basic unit. (biomedcentral.com)
The dissemination of tumor cells from the primary tumor to distant sites through bloodstream cannot be early detected by standard imaging methods. (amegroups.org)
Since then, with the evolution of the equipment and toolbox of molecular biology with methods such as single-cell next generation sequencing (NGS), the need for an even more detailed investigation of organisms at a single-cell level has emerged. (por-journal.com)
Currently, detection of breast cancer metastasis relies on clinical manifestations of the spread to distant organs, biopsies of affected organs, radiological evaluations, imaging methods and serum tumor markers ( 3 , 4 ). (iiarjournals.org)

Exosomes contain various molecular constituents of their cell of origin, including proteins and RNA. (exosome-rna.com)
Finally, exosomes are secreted from cells by exocytosis of the MVB contents. (molcells.org)

Immunomagnetic cell sorting also allowed us to identify and isolate potent TIL subpopulations, in particular TILs with moderate levels of CD39 (a marker of T-cell reactivity to tumours and T-cell exhaustion), which we found are tumour-specific, self-renewable and essential for the long-term success of adoptive cell therapies. (northwestern.edu)
It is critical to isolate SARS-CoV-2 viruses to characterize their ability to infect and replicate in multiple human cell types and to determine if the virus is evolving in its ability to infect human cells and cause severe disease. (cdc.gov)

Although immunohistochemistry is the staple methodology for identifying neuronal cell types, since it is relatively low in cost and a wide range of immunohistochemical markers are available to help distinguish the phenotype of cells in the brain, sometimes it is time-consuming to produce a good antibody. (wikipedia.org)
As proven in Table 1 there exists no consistent subset of surface mole cules which can be a definition with the MSCs phenotype, so it can be achievable that numerous slightly various subtypes exist, and that their output phenotypes could possibly be conditional upon area and systemic signalling. (plasignaling.com)
It is still unclear which molecular factors and cell types are involved in shaping neutrophil function and phenotype in the CLL microenvironment. (biomedcentral.com)
The obtained results underline the plasticity of the neutrophil phenotype, observed under the influence of leukemic cells alone and depending on the presence of Treg. (biomedcentral.com)

In doing so, CTCelect enabled automated dispensing of single circulating tumor cells from HNSCC patient samples, qPCR-based confirmation of tumor-related biomarkers with a simplified one-step protocol and immunostaining using a panel of tumor markers. (fraunhofer.de)
Emerging techniques using the analysis of circulating tumor cells show promising results in predicting and identifying the early stages of breast cancer metastasis in patients. (iiarjournals.org)
Circulating tumor cells in the blood stream. (iiarjournals.org)

Scientists report that self-renewing cancer stem cells (CSCs) in glioblastoma have low TLR4 expression that allows them to survive by disregarding inflammatory signals. (stemcellsciencenews.com)
Researchers used clinical samples and patient-derived glioblastoma stem cells (GSCs) to confirm that the double-strand break (DSB) repair protein RAD51 is highly expressed in GSCs, which are reliant on RAD51-dependent DSB repair after radiation. (stemcellsciencenews.com)
Researchers demonstrated that apoptosis inhibitor-5 (API5) confers cancer stem cell (CSC)-like properties, including NANOG expression, the frequency of CD44-positive cells and sphere-forming capacity. (stemcellsciencenews.com)
Recent research transplanting mesenchymal stem cells (MSC) showed their potential in MI therapy. (bvsalud.org)
Moreover, RNA-sequencing of samples from stem cell treated mice after re-infarction demonstrated a significant decline in most arrhythmias with reduced inflammatory pathways. (bvsalud.org)
Additionally, following stem-cell therapy we found numerous highly expressed genes to be either linked to lowering the risk of heart failure, cardiomyopathy or sudden cardiac death. (bvsalud.org)
In summary, our stem-cell therapy led to a reduction in cardiac arrhythmias after MI and showed a downregulation of already established inflammatory pathways. (bvsalud.org)
2007), steady engraftment, and success of transplanted embryonic stem cell (ESC)-produced neural cells (Barral et?al. (epf2013.org)
Risk-adapted therapies for AML, ALL, and CLL patients consist of chemotherapy with/without stem cell transplantation (SCT), but the rate of early failures and relapses is still unsatisfying. (nature.com)
human pluripotent stem cell (hPSC)-derived myoblasts may be cultured on cultureware coated with Corning® Matrigel® hESC-Qualified Matrix. (stemcell.com)
Very recently using a (marker gene knocked into the locus Kataoka reported the regulatory regions were active almost specifically in hematopoietic stem and progenitor cells (10). (acancerjourney.info)
They were expanded for three times in serum-free X-vivo 15 moderate (Lonza Basel Switzerland) filled with penicillin streptomycin glutamax (Sigma-Aldrich GF 109203X Seelze Germany) and 50 ng/ml of every fms-related tyrosine kinase 3 ligand (FLT3-L) stem cell aspect (SCF) and thrombopoietin (TPO) (Peprotech Hamburg Germany). (acancerjourney.info)
The concept of heterogeneity has been around for several decades and gained attention in the 1990s when cancer stem cells were identified in acute myeloid leukemia [ 2 ]. (por-journal.com)
There are two not mutually exclusive models explaining tumor heterogeneity: the cancer stem cell and the clonal evolution model. (por-journal.com)
In the former model, tumor cells are hierarchically organized: a portion of cells, called the "stem cells" retain their ability to proliferate, while their offspring "differentiate" into nonproliferating cells [ 3 ]. (por-journal.com)
Bone marrow-derived mesenchymal stem cells (BM-MSCs) are multipotent stromal cells that have a critical role in the maintenance of skeletal tissues such as bone, cartilage, and the fat in bone marrow. (ijbs.com)

It allows detection and identification of cells by using different techniques. (wikipedia.org)
Parasite clearance following treatment with antimalarial drugs resulted in decreased detection of HIV-1 particles derived from the CD14 macrophage cell subset and correlated with a marked diminution in systemic immune activation. (lww.com)
In-situ fluorescent immunomagnetic multiplex detection of foodborne pathogens in very low numbers. (docksci.com)

Since regulatory T cells (Treg) play an important role in CLL progression and influence the activity of neutrophils, we investigated the crosstalk between Treg and neutrophils in the spleen using a murine model of CLL. (biomedcentral.com)
Both MDSC (myeloid-derived suppressor cells) and regulatory T cells are recruited to CRC where they inhibit the anti-tumour immune response, thereby promoting tumour progression. (biomedcentral.com)
Some of these cells have antitumoral behavior (NK cells, CD8 + T cells, CD4 + Th1 cells, and APCs), while others can promote tumor progression (CD4 + Th2 cells, regulatory T cells, and tumor-associated macrophages) [ 13 ]. (por-journal.com)

We produced a fluorescent antibody as a single recombinant protein in Escherichia coli by fusing a red-shifted mutant of green fluorescent protein (EGFP) to a single-chain antibody variable fragment (scFv) specific for hepatitis B surface antigen (HepBsAg). (thenewsindependent.com)

The second technical challenge originates from the heterogeneity of tumor cells whose physical and natural attributes may differ considerably within CTC populations and as time passes. (conferencedequebec.org)
Our unique quality controls provide assay values for both normal and abnormal leukocyte populations and reference values for both intracellular and surface markers. (streck.com)

Here, we show that site directed CAR integration utilizing nanoplasmid DNA delivered via HITI provides high cell yields and highly functional cells. (biomedcentral.com)
Recent advances in genome sequencing of single microbial cells enable the assignment of functional roles to members of the human microbiome that cannot currently be cultured. (biomedcentral.com)
Diabetes is known to impair the number and function of endothelial progenitor cells in the circulation, causing structural and functional alterations in the micro- and macro-vasculature. (biomedcentral.com)

CD20 has been detected at low levels on a small subset of mature T cells. (thermofisher.com)

CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. (thermofisher.com)

Ricin causes toxicity by inhibiting the formation (synthesis) of proteins in the cells of the exposed individual. (cdc.gov)
Autophagy is a lysosome-mediated catabolic process that allows cells to degrade damaged organelles and misfolded proteins, and to recycle nutrients [ 8 ]. (biomedcentral.com)
On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin. (thenewsindependent.com)
GFP (D5.1) XP ® Rabbit mAb detects GFP, YFP, and CFP-tagged proteins exogenously expressed in cells. (thenewsindependent.com)

Applications Tested: This G8.8 antibody has been tested by flow cytometric analysis of the TE-71 cell line. (thermofisher.com)
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)

We applied homology-independent targeted insertion (HITI) or HDR using CRISPR/Cas9 and nanoplasmid DNA to insert an anti-GD2 CAR into the T cell receptor alpha constant (TRAC) locus and compared both targeted insertion strategies in our system. (biomedcentral.com)
CD326 mediates calcium-independent, homophilic, cell-cell adhesion and may function as a growth factor receptor. (thermofisher.com)
Qin R , Zhu H , Wang F , Song M , Lin P , Xing Y , Zhang W , Zhong M , Wang Z , . Platelet activation in diabetic mice models: the role of vascular endothelial cell-derived protein disulfide isomerase-mediated GP IIb/IIIa receptor activation. (aging-us.com)
In particular, Treg affect the expression of CD62L and IL-4 receptor in neutrophils, both of which are crucial for the function of these cells. (biomedcentral.com)
CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. (thermofisher.com)
After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. (thermofisher.com)
The SARS-CoV-2 gain access to the cells on the airway mucosa by concentrating on the ACE2 receptor. (enmd-2076.com)

Subsequent single-cell analyses after MI showed differences in macrophage development and a lower prevalence of CCR2 expressing macrophages. (bvsalud.org)
Cardiomyocyte transplantation increased NK cells and monocytes, while reducing CCR2-MHC-IIlo macrophages. (bvsalud.org)
The innate immune system includes macrophages, dendritic cells (DC), and natural killer (NK) cells, which respond quickly to an immunological threat. (nature.com)

Nowadays, with single-cell approaches, tumor heterogeneity, the tumor microenvironment, and the interplay between tumor cells and their surroundings can be described in unprecedented detail. (por-journal.com)

The purpose of this study was to investigate the release of Endothelial microparticle (EMP)-associated protein disulfide isomerase (PDI) after endothelial cell injury induced in diabetes and the changes in platelet activation. (aging-us.com)

Ensure high yields when passaging and culturing your primary human skeletal muscle progenitor cells (myoblasts) by using MyoCult™-SF Attachment Substrate, which is optimized to support myoblast attachment. (stemcell.com)
For more information on protocols for the derivation and expansion of human skeletal muscle progenitor cells with MyoCult™, please explore the Product Information Sheet . (stemcell.com)

Indeed, neutrophils are able to support leukemia progression through mechanisms that mimic the cooperation of neutrophils with healthy regulatory B cells [ 6 ]. (biomedcentral.com)

Here we show that a configurable microfluidic device can efficiently recover potent TILs from solid tumours by leveraging specific expression levels of target cell-surface markers. (northwestern.edu)
Here we compared two microfluidic devices for the recovery of circulating melanoma cells. (nature.com)

2007) as mesDA progenitor-associated cell surface area markers. (epf2013.org)
Surface area markers portrayed on 90% from the FOXA2+ mesDA progenitor cells are depicted within the Edwards-Venn diagram (Bardou et?al. (epf2013.org)
Twelve surface area markers had been concomitantly portrayed on d11 and d16 both in hESC and hiPSC-derived FOXA2+ cells. (epf2013.org)
As proven for IAP, surface area markers portrayed by mesDA progenitors had been identified in line with the co-staining with FOXA2. (epf2013.org)

An ideal marker is specific to a given cell type in normal conditions and/or during injury. (wikipedia.org)
Using this technique, it is possible to locate gene expression to specific cell types in specific regions and observe how changes in this distribution occur throughout the development and correlate with the behavioral manipulations. (wikipedia.org)
Furthermore, it can define the metabolic features and pathogenic potential of specific bacterial cells and can indicate whether they contain phage and plasmids that facilitate horizontal transfer of genes for clinically relevant traits, such as antibiotic resistance. (biomedcentral.com)
After RBC depletion, a cell sample is ready to be separated into specific cell subsets or otherwise undergo further processing. (akadeum.com)
GFP- and by inference Evi1- positive cells were strongly enriched for cells with multilineage and long-term repopulation potential suggesting that was a highly specific and discriminative marker for murine HSCs. (acancerjourney.info)
Kiehne K, Folsch UR, Nitsche R. High cell language of AX Safety products in narratives with specific broad minute hypothalamic to livre. (onecnctraining.com)

Total viable cells, as determined by Fixable Viability Dye eFluor® 450, were used for analysis. (thermofisher.com)
Microbubbles have no negative effect on cell viability. (akadeum.com)

In conclusion, EMP-PDI promotes early platelet activation through glycoprotein (GP) IIb/IIIa receptors present on platelet surface in the diabetic state. (aging-us.com)
However, the ultimate common pathway of platelet aggregation is the activation of glycoprotein (GP) IIb/IIIa receptors present on platelet surface [ 7 - 9 ]. (aging-us.com)

PDF) Kraan J, van den Broek P, Verhoef C, Grunhagen DJ, Taal W, Gratama JW, Sleijfer SEndothelial CD276 (B7-H3) expression is increased in human malignancies and distinguishes between normal and tumour-derived circulating endothelial cells. (researchgate.net)
Melanoma tumour cells exhibit pronounced heterogeneity within a single tumour, amongst concurrent tumours within the same patient and amongst tumours from different patients. (nature.com)

GFP is a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression. (thenewsindependent.com)

Gene expression profile was assessed using single-cell and bulk RNA sequencing. (bvsalud.org)
Several researchers have applied single cell clones as a source of trilineage potent MSCs, whereas many others have shown their potential by in vivo analysis in xenografts. (plasignaling.com)
Now, single-cell genomics of microbial cells has become possible in recent years and offers a solution to this limitation. (biomedcentral.com)
Overview of steps for single-cell sequencing of microbial genomes. (biomedcentral.com)
Conventional genome-assembly algorithms often have problems with single-cell data because they assume that chimeras are rare and genome coverage is Poisson distributed. (biomedcentral.com)
Creative Proteomics is a leading service provider focused on subcellular and single-cell level research. (creative-proteomics.com)
Furthermore GFP-positive progenitor cells yielded higher numbers of highly proliferative colonies in GF 109203X single-cell tradition and more colonies in semisolid press than their GFP-negative counterparts (10). (acancerjourney.info)

BACKGROUND: The immune response is a crucial factor for mediating the benefit of cardiac cell therapies. (bvsalud.org)
Thus, clinicians can use cell therapies worldwide to treat PAD. (bvsalud.org)
Our work provides a novel platform to perform guided CAR insertion into primary human T-cells using nanoplasmid DNA and holds the potential to increase access to CAR-T cell therapies. (biomedcentral.com)

Tumor heterogeneity means that neoplastic cells from the same tumor can genotypically, phenotypically, morphologically, or metabolically differ from each other. (por-journal.com)
Heterogeneity in solid tumors is not limited to the differences between neoplastic cancer cells. (por-journal.com)
While BM-MSCs have high cell-to-cell heterogeneity in gene expression, the cell subtypes that contribute to this heterogeneity in vivo in humans have not been characterized. (ijbs.com)

Results: No difference in antigen expression between normal and malignant endothelial cells (ECs) was found for CD54, CD109, CD137, CD141, CD144 and CXCR7. (researchgate.net)

Anatomy43

Organisms15

Diseases11

Chemicals and Drugs64

Analytical, Diagnostic and Therapeutic Techniques and Equipment44

Phenomena and Processes36

Disciplines and Occupations5

Technology, Industry, Agriculture5

Information Science3

Health Care6

Isolation21

Enrichment8

Beads5

Epithelial9

Antibodies3

FACS4

Separation2

Proliferation6

Cytokines7

Mononuclear cells4

Therapeutic8

Lymphocytes5

Differentiation7

CTCs13

Peripheral blood4

Flow cytometry7

Fluorescence2

Extracellular Vesicles5

Immune cells6

EpCAM1

Progenitor cell3

Magnetic3

Cellular4

Vesicles1

Biological5

Methods4

Exosomes2

Isolate2

Phenotype4

Circulating Tumor Cells3

Stem16

Detection3

Regulatory T cel3

Fluorescent1

Populations2

Functional3

Subset1

Molecular1

Proteins4

Antibody2

Receptor7

Macrophages3

Approaches1

Endothelial cell1

Human skeletal muscle2

Progression1

Microfluidic2

Area markers4

Specific6

Viability2

Receptors2

Tumour2

Physiological1

Single-cell7

Therapies3

Heterogeneity3

Antigen expression1