AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPsychiatry and PsychologyPhenomena and ProcessesDisciplines and OccupationsInformation ScienceHealth Care
Pigment Epithelium of EyeRetinal Pigment EpitheliumChoroidLipofuscinBruch MembraneRetinal PigmentsRetinaMacular DegenerationEpitheliumcis-trans-IsomerasesEye ProteinsPigments, BiologicalRetinal DegenerationIodatesFluorescein AngiographyRetinal DiseasesFundus OculiRetinal DetachmentPhotoreceptor CellsPhotoreceptor Cells, VertebrateElectroretinographyChoroidal NeovascularizationEyeRetinal DrusenMacula LuteaFluorescent Antibody Technique, IndirectRetinaldehydeRetinoidsRod Cell Outer SegmentMelanosomesTomography, Optical CoherenceBlood-Retinal BarrierCells, CulturedMelaninsChoroid DiseasesOphthalmoscopyDark AdaptationVitreous BodyRetinal Photoreceptor Cell Outer SegmentAlbinismRats, Mutant StrainsIrisChoroideremiaRetinol-Binding ProteinsLightGeographic AtrophyVitreoretinopathy, ProliferativeMicrophthalmia-Associated Transcription FactorPyridinium CompoundsCiliary BodyEpithelial CellsVitamin AReverse Transcriptase Polymerase Chain ReactionRetinal PerforationsCattleRNA, MessengerPigmentationVisual AcuityBlotting, WesternPhagocytosisMicroscopy, ElectronRetinal Cone Photoreceptor CellsMicrophthalmosMice, Inbred C57BLRod OpsinsLaser CoagulationSerpinsRetinitis PigmentosaIndocyanine GreenImmunohistochemistryRetinal NeuronsRhodopsinInjectionsCell LineOptic Disk DrusenRana catesbeianaHistocytochemistryAlbinism, OcularRetinal VesselsMicroscopy, ConfocalImmunoenzyme TechniquesTrypan BlueWet Macular DegenerationRetinal DystrophiesBile PigmentsMelanocytesRetinal Rod Photoreceptor CellsAgingOtx Transcription FactorsMicroscopy, FluorescenceEye DiseasesCarrier ProteinsUveaCell TransplantationFetal Tissue TransplantationGene ExpressionFluorescenceOphthalmia, SympatheticChoroid NeoplasmsSclera
Retina and Retinal Pigment EpMacularDegenerationActivated during retinal deveTherapeuticTransplantationMutationsPhotoreceptorsGeneChoroidRPE65Variety of retinalDiseasesMelaninMonolayerMaculaHypertrophyNeuralNeuronsDisordersLesionsEpithelial cellDiabeticMechanismsExperimental2017ProliferationDetachmentMetabolismTissueMembranesDifferentiation of humanGenesPhenotypeStemOptic nerveTissuesBlood-retinalOuterVitreousMelanosomesIntraretinalMigrationAtrophyGeneticAbnormalClinicalProgenitorBarrierGanglion cellDiseaseMethodsProteinRegenerateOcularCystoidPhysiology
Retina and Retinal Pigment Ep1
Macular13
Degeneration7
  • The acute model of light-induced retinal degeneration uses short exposure to bright white light to study photoreceptor cell death leading to loss of vision. (nature.com)
  • We also identified other genes, including IDO1 and BAMBI, that may influence the RPE and therefore outer blood-retinal barrier integrity during ocular infection and inflammation, or are associated with degeneration, as seen for example in aging. (molvis.org)
  • Most retinal diseases involve the degeneration of choroidal retinal pigment epithelial (RPE) cells . (bvsalud.org)
  • See the clinical guideline summary from the American Academy of Ophthalmology, Posterior vitreous detachment, retinal breaks, and lattice degeneration . (medscape.com)
  • Conditional Loss of the Exocyst Component Exoc5 in Retinal Pigment Epithelium (RPE) Results in RPE Dysfunction, Photoreceptor Cell Degeneration, and Decreased Visual Function. (nih.gov)
  • Punzo C ., Xiong W., Cepko C.L. Loss of daylight vision in retinal degeneration: are oxidative stress and metabolic dysregulation to blame? (umassmed.edu)
  • What is pigment degeneration? (xshotpix.com)
Activated during retinal deve1
  • However, promoters of these genes were demethylated and activated during retinal development. (nih.gov)
Therapeutic9
  • Induced pluripotent stem (iPS) cells have the potential to differentiate into any cell type, making them a potential source from which to produce cells as a therapeutic platform for the treatment of a wide range of diseases. (ca.gov)
  • Cultured retinal pigment epithelium (RPE) may become a therapeutic option for transplantation in retinal disease. (allergy-link.com)
  • Human parthenogenetic stem cells have great therapeutic potential, yet do not require viable human embryos, thus avoiding ethical issues. (blogspot.com)
  • Creating experimental retinal detachments with a reproducible and sustained height of detachment, and without subretinal hemorrhage, is important for studying the pathophysiology of photoreceptor cell loss in retinal disease and evaluating potential therapeutic interventions. (jove.com)
  • Furthermore, the ability to isolate and expand cells of sufficient therapeutic quality and quantity in a patient-to-patient model can limit progress. (scienceboard.net)
  • Techniques such as cellular reprogramming will allow the exploration of scalable allogeneic cell sources and the optimizable generation of cells for therapeutic development. (scienceboard.net)
  • This circumvents the need for any intermediary steps and reduces the time required to produce a cell type for therapeutic use. (scienceboard.net)
  • First-generation ex vivo cell therapies, such as autologous anti-CD19 CAR T cells, demonstrated the clinical value and curative potential of enhancing the therapeutic qualities of immune and other patient-derived cell types. (scienceboard.net)
  • Molecule screening on human motor neurons produced from pluripotent stem cells helped identify the therapeutic potential of lithium in this genetic form of autism. (afm-telethon.fr)
Transplantation6
  • Protective effects of human iPS-derived retinal pigment epithelium cell transplantation in the retinal dystrophic rat. (ca.gov)
  • Our results demonstrate that iPS cells can be differentiated into functional iPS-RPE and that transplantation of these cells can facilitate the short-term maintenance of photoreceptors through phagocytosis of photoreceptor outer segments. (ca.gov)
  • Mesenchymal stem cell transplantation and DMEM administration in a 3NP rat model of Huntington's disease: morphological and behavioral outcomes. (allergy-link.com)
  • Transplantation of mesenchymal stem cells (MSCs) may offer a viable treatment for Huntington's disease (HD). (allergy-link.com)
  • Fibrin hydrogels as a xenofree and rapidly degradable support for transplantation of retinal pigment epithelium monolayers. (mayo.edu)
  • Prior to the invention of CAR, cell therapies were largely limited to allogeneic hematological transfusion and transplantation. (scienceboard.net)
Mutations3
  • Mutations in the RDS gene disrupt the structures in these cells that contain light-sensing pigments, leading to vision loss. (wikipedia.org)
  • Grill C, Bergsteinsdottir K, Ogmundsdottir MH, Pogenberg V, Schepsky A, Wilmanns M, Pingault V, Steingrimsson E. MITF mutations associated with pigment deficiency syndromes and melanoma have different effects on protein function. (medlineplus.gov)
  • Epidemiology of Mutations in the 65-kDa Retinal Pigment Epithelium (RPE65) Gene-Mediated Inherited Retinal Dystrophies: A Systematic Literature Review. (cdc.gov)
Photoreceptors5
  • We show here that the neural cell adhesion molecule (N-CAM) is found both on the apical surface of RPE in situ and on the outer segments of photoreceptors, fulfilling an important requisite for an adhesion role between both structures. (rupress.org)
  • This results in atrophy and death of the RPE cells and subsequently the photoreceptors leading to central blindness. (frontiersin.org)
  • Although the overlying neural tissue is partly transparent, and the accompanying glial cells have been shown to act as fibre-optic channels to transport photons directly to the photoreceptors, [7] [8] light scattering does occur. (wikipedia.org)
  • Although their photoreceptors contain a protein, retinochrome, that recycles retinal and replicates one of the functions of the vertebrate RPE, cephalopod photoreceptors are likely not maintained as well as in vertebrates, and that as a result, the useful lifetime of photoreceptors in invertebrates is much shorter than in vertebrates. (wikipedia.org)
  • It regulates many physiologic processes that include cell survival, migration, differentiation, and PHAGOCYTOSIS of apoptotic cells and ROD PHOTORECEPTORS in the RETINAL PIGMENT EPITHELIUM. (bvsalud.org)
Gene15
  • Best disease is inherited in an autosomal dominant pattern, which means one copy of the altered gene in each cell is sufficient to cause the disorder. (wikipedia.org)
  • This gene provides instructions for making a protein that plays a role in the development, survival, and function of certain types of cells. (medlineplus.gov)
  • Discoveries in fundamental basic research have revealed how human embryonic stem cells regulate gene expression to maintain pluripotency, how induced pluripotent stem cells retain a "memory" of their origin, and how adult stem cells differentiate in response to their environmental milieu. (ca.gov)
  • Our study demonstrates a circadian rhythm of contrast sensitivity that peaks during the daytime, and that its regulation involves interactions of D4Rs, the clock gene Npas2 , and the clock-controlled gene adenylyl cyclase 1 ( Adcy1 ) in a subset of retinal ganglion cells (RGCs). (jneurosci.org)
  • Targeted disruption of the gene encoding D4Rs reduces the amplitude of the contrast sensitivity rhythm by reducing daytime sensitivity and abolishes the rhythmic expression of Npas2 and Adcy1 mRNA in the ganglion cell layer (GCL) of the retina. (jneurosci.org)
  • DFNB2 is inherited in an autosomal recessive pattern, which means both copies of the MYO7A gene are mutated in each cell. (medlineplus.gov)
  • Gene Therapy Approaches for the Treatment of Retinal Disorders. (umassmed.edu)
  • Retinal Gene Delivery by rAAV and DNA Electroporation. (umassmed.edu)
  • Cellular reprogramming involves remodeling gene expression to generate a desired cell lineage or type. (scienceboard.net)
  • The "spotlights" represent the use of single-cell RNA sequencing to reveal the gene expression state of cells at early, mid, and late time points of retinal pigment epithelium differentiation. (ophthalmologytimes.com)
  • Using scRNA-seq, the researchers were able to study the entire gene expression profile of individual human embryonic stem cells throughout the differentiation protocol, which takes a total of sixty days. (ophthalmologytimes.com)
  • AFM-Telethon has supported and continues to support the development (clinical trials underway or in preparation) of over forty drugs in gene therapy, cell therapy and pharmacology. (afm-telethon.fr)
  • Strimvelis® (gene therapy of the bone marrow stem cells) for X-linked severe combined immune deficiency. (afm-telethon.fr)
  • Zynteglo® (gene therapy of the bone marrow stem cells) for beta thalassemia. (afm-telethon.fr)
  • Economic Impact of Next-Generation Sequencing Versus Single-Gene Testing to Detect Genomic Alterations in Metastatic Non-Small-Cell Lung Cancer Using a Decision Analytic Model. (cdc.gov)
Choroid3
RPE651
  • Voretigene neparvovec -rzyl is now available for the treatment of confirmed biallelic RPE65 mutation-associated retinal dystrophy. (msdmanuals.com)
Variety of retinal1
  • At these annual meetings, there will be 20 scientific presentations on MicroPulse laser therapy that utilize IRIDEX's Cyclo G6® Glaucoma Laser and MicroPulse P3® Delivery Device for treating multiple types of glaucoma, and IRIDEX's IQ family of laser consoles for treating a variety of retinal disorders. (iridex.com)
Diseases3
  • These findings have identified an alternative source of replacement tissue for use in human retinal cellular therapies, and provide a new in vitro cellular model system in which to study RPE diseases affecting human patients. (ca.gov)
  • Degradation of photoreceptor outer segments (POS) by retinal pigment epithelium (RPE) is essential for vision, and studies have implicated altered POS processing in the pathogenesis of some retinal degenerative diseases. (wisc.edu)
  • ISCO's scientists are focused on using hpSC to treat severe diseases of the eye, the nervous system and the liver where cell therapy has been proven clinically yet is limited by the availability of safe immune-matched human cells. (blogspot.com)
Melanin9
Monolayer6
  • As a monolayer of cells critical to photoreceptor function and survival, the RPE is an ideally accessible target for cellular therapy. (ca.gov)
  • QBAM images of iPSC-RPE were used to train DNNs that predicted iPSC-RPE monolayer transepithelial resistance, predicted polarized vascular endothelial growth factor (VEGF) secretion, and matched iPSC-RPE monolayers to the stem cell donors. (jci.org)
  • ARPE-19 cells were able to form a monolayer on the surface of the membrane and no cell invasion into the membrane was seen. (frontiersin.org)
  • This could allow this novel bioactive substrate to be a potential treatment for atrophic AMD that would function two-fold: deliver the required monolayer of healthy RPE cells to the macula on a synthetic BM and remove diseased structures within the retina, restoring the waste/exchange pathway and preventing vision loss. (frontiersin.org)
  • The requirements for the successful culture of organoids in vitro differ significantly from those of traditional monolayer cell cultures. (mdpi.com)
  • The retinal pigment epithelium (RPE) is a monolayer of pigmented cells situated between the neuroretina and the choroids. (xshotpix.com)
Macula4
  • This disorder affects the retina, specifically cells in a small area near the center of the retina called the macula. (wikipedia.org)
  • The macula is part of the eye's retina, which is the light-sensitive tissue mostly composed of the eye's visual cells: cone and rod photoreceptor cells. (ophthalmologytimes.com)
  • Wet AMD occurs when abnormal blood vessels form underneath the macula - the section of the retina that allows detailed, central vision - and damage its cells. (medscape.com)
  • Wet AMD is more serious than dry AMD, which is more common and caused by cells in the macula being damaged by a deposit of fatty protein. (medscape.com)
Hypertrophy2
Neural7
  • Apical polarization of N-CAM in retinal pigment epithelium is dependent on contact with the neural retina. (rupress.org)
  • The retinal pigment epithelium (RPE) is unique among epithelia in that its apical surface does not face a lumen, but, instead, is specialized for interaction with the neural retina. (rupress.org)
  • Glial remodeling and neural plasticity in human retinal detachment with proliferative vitreoretinopathy. (medscape.com)
  • The neural retina consists of several layers of neurons interconnected by synapses and is supported by an outer layer of pigmented epithelial cells. (wikipedia.org)
  • Neural signals from the rods and cones undergo processing by other neurons, whose output takes the form of action potentials in retinal ganglion cells whose axons form the optic nerve. (wikipedia.org)
  • All neuroectoderm-derived neural cells exhibited a high percentage of the mutant variant. (jci.org)
  • Reduces colony-forming efficiency of mouse neural stem cells (Androutsellis-Theotokis et al. (stemcell.com)
Neurons4
  • The vertebrate retina is inverted in the sense that the light-sensing cells are in the back of the retina, so that light has to pass through layers of neurons and capillaries before it reaches the photosensitive sections of the rods and cones. (wikipedia.org)
  • Promotes differentiation of neurons from human and mouse embryonic stem (ES) cells (Crawford and Roelink, Elkabetz et al. (stemcell.com)
  • Exocyst Complex Member EXOC5 Is Required for Survival of Hair Cells and Spiral Ganglion Neurons and Maintenance of Hearing. (nih.gov)
  • Kanadia R.N., Clark V.E., Punzo C., Trimarch J., Cepko C.L. Temporal requirement of the alternative splicing factor Sfrs1 for the survival of retinal neurons. (umassmed.edu)
Disorders1
  • Wang M, Li H, Wang F. Roles of Transepithelial Electrical Resistance in Mechanisms of Retinal Pigment Epithelial Barrier and Retinal Disorders. (medscape.com)
Lesions2
  • CONCLUSIONS: AMD results in an altered RPE with enlarged and deformed cells that could precede clinically visible lesions and thus serve as early biomarkers for AMD onset. (nevinmanimala.com)
  • iRORA, for Incomplete RPE and Outer Retinal Atrophy, refers to pre-GA lesions, while cRORA refers to Com-plete RPE and Outer Retinal Atrophy. (aao.org)
Epithelial cell2
  • Retinal pigment epithelial cell. (nih.gov)
  • Acts upstream of with a negative effect on epithelial cell apoptotic process. (nih.gov)
Diabetic2
  • Coral K, Angayarkanni N, Gomathy N, Bharathselvi M, Pukhraj R, Rupak R. Homocysteine levels in the vitreous of proliferative diabetic retinopathy and rhegmatogenous retinal detachment: its modulating role on lysyl oxidase. (medscape.com)
  • Is the retinal pigment epithelium involved in diabetic retinopathy? (xshotpix.com)
Mechanisms4
  • In this study, we investigated the epigenetic plasticity of adult murine RPE to identify possible mechanisms that prevent mammalian RPE from regenerating retinal tissue. (nih.gov)
  • This research was conducted to detail the mechanisms of these effects of lycopene-treated RPE cells. (nih.gov)
  • Research carried out in the facility has made significant, high impact contributions in three areas of stem cell research: Molecular Mechanisms/Basic Science, Translational Bioengineering, and Regenerative Medicine. (ca.gov)
  • However, the underlying cellular and molecular mechanisms through which retinal dopamine modulates contrast sensitivity have not been elucidated. (jneurosci.org)
Experimental3
  • The aim of this experimental study was to examine potentially cytotoxic effects of ICG and TB on cultured human RPE cells. (uni-tuebingen.de)
  • Experimental studies on embryonic mouse and chick eyes have shown that the ILM is a critical component of retinal histogenesis and optic axonal growth and navigation to the optic disc. (hindawi.com)
  • Our presentation discusses the technique in great deal and discusses experimental results using retinal pigment epithelium cells. (aps.org)
20171
  • The underlying native diseased BM and related structures remain, however, which leaves the potential for healthy transplanted cells to eventually succumb to disease ( White and Olabisi, 2017 ). (frontiersin.org)
Proliferation3
  • The results obtained from this work showed that the proliferation of MG63 cell was more significant in the group containing ionic products of Ca(2)SiO(4) coatings than in the control group . (allergy-link.com)
  • Proliferative vitreoretinopathy can occur from glial or retinal pigment epithelium (RPE) proliferation. (medscape.com)
  • The ILM serves as a scaffold for cellular proliferation of myofibroblasts, fibrocytes, and retinal pigment epithelium (RPE) cells [ 2 ]. (hindawi.com)
Detachment6
Metabolism2
  • The presence of both PLGA and PGA nanoparticles increased ARPE-19 cell metabolism but had no effect on cell viability. (frontiersin.org)
  • Improved cell metabolism prolongs photoreceptor survival upon retinal-pigmented epithelium loss in the sodium iodate induced model of geographic atrophy. (umassmed.edu)
Tissue8
  • DNN predictions were supplemented with traditional machine-learning algorithms that identified shape and texture features of single cells that were used to predict tissue function and iPSC donor identity. (jci.org)
  • An organoid is a 3D organization of cells that can recapitulate some of the structure and function of native tissue. (mdpi.com)
  • Here, we identify nonrandom distribution of a pathogenic mtDNA variant across a complex tissue using single-cell RNA-Seq, mitochondrial single-cell ATAC sequencing, and multimodal single-cell sequencing. (jci.org)
  • The essential role of the ILM to the integrity of the retina and risk of trauma to retinal tissue spurs suspicion with regard to its routine removal. (hindawi.com)
  • These have variable effectiveness in different tissues, with some tissue types retaining all of their senescent cells, suggesting that no initial clinical treatment is going to be perfect. (fightaging.org)
  • In large numbers senescent cells cause chronic inflammation and their collective signaling actively harms tissue structure and function. (fightaging.org)
  • Limitations in the usage of AAV in murine models include cell types that are refractory to transduction and difficulties achieving tissue-specificity. (nature.com)
  • Plasma Cell-Free DNA Testing of Patients With EGFR Mutant Non-Small-Cell Lung Cancer: Droplet Digital PCR Versus Next-Generation Sequencing Compared With Tissue-Based Results. (cdc.gov)
Membranes2
  • 6 , 7 High photon flux, oxygen tension and the high levels of polyunsaturated fatty acids present in rod outer segment membranes make rod photoreceptor cells especially vulnerable to photochemical damage. (nature.com)
  • Histologically, adenomas in this layer typically retain characteristics of RPE cells, including basement membranes, cell junctions, and microvilli. (aao.org)
Differentiation of human1
  • Here we report the differentiation of human iPS cells into RPE. (ca.gov)
Genes7
  • To identify key genes differentially expressed in the human retinal pigment epithelium (hRPE) following low-level West Nile virus (WNV) infection. (molvis.org)
  • RNA extracted from mock-infected and WNV-infected cells was assessed for differential expression of genes using Affymetrix microarray. (molvis.org)
  • Low-level WNV infection of hRPE cells induced expression of genes that are typically associated with the host cell response to virus infection. (molvis.org)
  • The process called parthenogenesis uses unfertilized human eggs and results in the cells inheriting a duplicate set of human leukocyte antigen (HLA) genes. (blogspot.com)
  • Unlike induced pluripotent stem cells (iPS), hpSC do not require the transfection of genes into somatic cells with the associated possibility of introducing unwanted additional genes. (blogspot.com)
  • The development of transgenic mouse models that express genes of interest in specific cell types has transformed our understanding of basic biology and disease. (nature.com)
  • Instead, the release noted that the researchers used a technique called single-cell RNA sequencing (scRNA-seq), which can detect all the active genes in an individual cell at a given time. (ophthalmologytimes.com)
Phenotype1
  • However, the landscape of heteroplasmy across cell types within tissues and its influence on phenotype expression in affected patients remains largely unexplored. (jci.org)
Stem36
  • Transformation of somatic cells with a set of embryonic transcription factors produces cells with the pluripotent properties of embryonic stem cells (ESCs). (ca.gov)
  • Gamm's laboratory at the Waisman Center utilizes stem cell technology to investigate the cellular and molecular events that occur during human retinal differentiation and generate cells for use in human retinal disease modeling and cell-based rescue or replacement strategies. (wisc.edu)
  • The methodology was validated using clinical-grade induced pluripotent stem cell-derived retinal pigment epithelial cells (iPSC-RPE). (jci.org)
  • In stem cell research, successes have been very few. (indiatimes.com)
  • Human Fibrinogen for Maintenance and Differentiation of Induced Pluripotent Stem Cells in Two Dimensions and Three Dimensions. (mayo.edu)
  • Stem Cells Transl Med. (mayo.edu)
  • Disease modeling studies using induced pluripotent stem cells: are we using enough controls? (mayo.edu)
  • Now what is also interesting here is the launch of "stem cell" cosmetics as another part of their business model, with retail sales on the web. (blogspot.com)
  • Recently I posted about "spray on" stem cell use that is about ready for clinical trials , so I guess to assume, the cosmetic end of the business is ready to advance as well. (blogspot.com)
  • If you read further down there's a bit of an explanation of the 4 types of stem cell technologies. (blogspot.com)
  • International Stem Cell Corporation (OTCBB:ISCO) develops a powerful new stem cell technology called "parthenogenesis" that promises to significantly advance the field of regenerative medicine by addressing the significant problem of immune-rejection. (blogspot.com)
  • Parthenogenesis utilizes unfertilized human eggs to create "parthenogenetic" stem cells (hpSC) that can be "immune-matched" to millions of persons of differing sexes and racial backgrounds. (blogspot.com)
  • UniStemCell bank is the life science industry's first collection of non-embryonic histocompatible human stem cells available for research and commercial use. (blogspot.com)
  • International Stem Cell Corporation (OTCBB:ISCO) has developed a proprietary technique for creating histocompatible stem cells. (blogspot.com)
  • Unlike human embryonic stem cells (hESC), hpSC do not require viable human embryos, thus avoiding most ethical concerns. (blogspot.com)
  • The chart below explains in brief the 4 types of stem cell processes. (blogspot.com)
  • In following some of the recent developments with stem cells there's a big move starting to continue and further advance stem cell storage areas around the world. (blogspot.com)
  • The cosmetic end of the business has their own website as well which explains their stem cell technology serums and processes. (blogspot.com)
  • The products state they use patented non-embryonic stem cell extract to help rejuvenate your skin. (blogspot.com)
  • The University of California at Santa Barbara (UCSB) is well positioned to make significant contributions by leveraging fundamental stem cell-based biomedical research efforts with enabling technologies in materials, microfluidics and bioengineering. (ca.gov)
  • We have developed and renovated a 1400 sq. ft. shared-use stem cell laboratory called the Laboratory for Stem Cell Biology and Engineering. (ca.gov)
  • The overall mission of this laboratory is to facilitate and promote stem cell research by investigators at UCSB, as well as those at neighboring universities, research institutes, and biotechnology companies on the California central coast. (ca.gov)
  • Past and current users of the Laboratory for Stem Cell Biology and Engineering include 52 researchers from 18 research groups, representing collaborations with 12 companies and 17 different universities and research institutes. (ca.gov)
  • Advances in bioengineering have addressed problems of how to control growth and differentiation of stem cells using novel biomaterials, how to sort and purify specific stem cell products, and how to deliver and sustain stem cell grafts in vivo. (ca.gov)
  • Past and current users of the Laboratory for Stem Cell Biology and Engineering include 58 researchers (6 new researchers in the past year) from 18 research groups, representing collaborations with 12 companies and 17 different universities and research institutes. (ca.gov)
  • Enhances radiation-induced cell death of glioma stem cells (Wang et al. (stemcell.com)
  • Promotes differentiation of nociceptors from human pluripotent stem cells, in combination with several other small molecules (Chambers et al. (stemcell.com)
  • Promotes differentiation of pancreatic cells from human pluripotent stem cells (D'Amour et al. (stemcell.com)
  • We demonstrate that transgenic AAVR overexpression greatly increases the efficiency of transduction of many diverse cell types, including muscle stem cells, which are normally refractory to AAV transduction. (nature.com)
  • This concept gained prominence following Shinya Yamanaka's discovery of induced pluripotent stem cells (iPSCs) in 2007. (scienceboard.net)
  • Scientist have proposed several methods for converting stem cells into RPE, but there is still a gap in our knowledge of how cells respond to these stimuli over time. (ophthalmologytimes.com)
  • La Manno has now led a study with Professor Fredrik Lanner at the Karolinska Institute (Sweden) profiling a protocol for differentiating human embryonic stem cells into RPE cells that is actually intended for clinical use. (ophthalmologytimes.com)
  • The study is published and featured on this month's cover of the journal Stem Cell Reports . (ophthalmologytimes.com)
  • A phase I/II trial by allogeneic mesenchymal stem cells for patients with systemic lupus erythematosus with kidney damage resistant to traditional treatments is underway. (afm-telethon.fr)
  • Results of a study published in the journal Nature Biotechnology describe how stem cell therapy can restore sight. (medscape.com)
  • Commenting on the trial in a statement, Dr Carmel Toomes, associate professor at the Leeds Institutes of Molecular Medicine, says: "These results give the many patients out there who suffer from AMD and other retinal degenerations real hope that stem cells replacement therapy may be a reality in the near future. (medscape.com)
Optic nerve2
  • therefore, the optic nerve must cross through the retina en route to the brain. (wikipedia.org)
  • Blood vessels (retinal artery and vein) travel along with the optic nerve and enter and exit through the back of the eye. (peacehealth.org)
Tissues5
  • Lately, there are growing concerns in terms of safety to retinal tissues, especially to the retinal pigment epithelium (RPE). (uni-tuebingen.de)
  • The wide range of manifestations observed in patients with mitochondrial disease results from varying fractions of abnormal mtDNA molecules in different cells and tissues, a phenomenon termed heteroplasmy. (jci.org)
  • Even these prototypes are, however, clearing as much as a quarter of senescent cells in some tissues. (fightaging.org)
  • Thus, naively, a hypothetical highly efficient senescent cell clearance therapy might work just fine in a 40-something adult, with tissues containing comparatively few senescent cells, while having a strong chance of killing patients in their 70s, with tissues containing many more senescent cells and also possessed of less resilient organs. (fightaging.org)
  • We hypothesized that AAV transgene expression could be targeted to specific tissues or cell types by selectively overexpressing AAVR. (nature.com)
Blood-retinal1
  • Collectively, our data suggest that exposure of R91W;Nrl −/− mice to blue light not only induces cone cell death but also disrupts the inner blood-retinal barrier. (nature.com)
Outer3
  • While exposure of wt mice resulted in massive pyknosis in a focal region of the outer nuclear layer (ONL), the exposure of R91W;Nrl −/− mice led to additional cell death detected within the inner nuclear layer. (nature.com)
  • ARPE-19 cells maintained in this medium could also phagocytose human photoreceptor outer segments (POS). (allergy-link.com)
  • The RPE cells have fine hair-like projections on their inner surface called microvilli which lie between and ensheath the tips of the photoreceptor outer segments. (answersingenesis.org)
Vitreous3
  • The internal limiting membrane (ILM), the basement membrane of the Müller cells, serves as the interface between the vitreous body and the retinal nerve fiber layer. (hindawi.com)
  • The back two-thirds of the inner wall of the vitreous chamber is lined with a special layer of cells (the retina): millions of highly sensitive nerve cells that convert light into nerve impulses. (peacehealth.org)
  • Diagnosis is by funduscopy, which shows pigmentation in a bone-spicule configuration in the equatorial retina, narrowing of the retinal arterioles, a waxy pallor of the optic disk, posterior subcapsular cataracts, and cells in the vitreous. (msdmanuals.com)
Melanosomes2
Intraretinal1
  • In our series of 7 cases, we documented impaired visual acuity, central visual field defects, circumscribed and sometimes complex changes of retinal reflectivity, and intraretinal fluid. (aerzteblatt.de)
Migration2
Atrophy1
  • Recently, the Classification of Atrophy Meetings (CAM) group, a collaboration among interna-tional experts in retinal imaging, published two reports: one suggested new terminology based on OCT findings, 1 while the other validated these terms as biomarkers for future research. (aao.org)
Genetic2
  • To meet these goals, Gamm utilizes a variety of human cell types, including ES and iPS cells, which have the capacity to mimic retinal development and disease, as well as to delineate the genetic "checkpoints" necessary to produce particular retinal cell types. (wisc.edu)
  • Multiple different methods have been demonstrated to selectively kill senescent cells in mice, including the genetic engineering approach used a few years ago and the various senolytic drug candidates discovered more recently. (fightaging.org)
Abnormal2
  • Over time, the abnormal accumulation of this substance can damage the cells that are critical for clear central vision. (wikipedia.org)
  • Dimers incorporating the abnormal MITF protein cannot be transported into the cell nucleus to bind with DNA. (medlineplus.gov)
Clinical5
  • Increases in the number of cell therapies in the preclinical and clinical phases have prompted the need for reliable and noninvasive assays to validate transplant function in clinical biomanufacturing. (jci.org)
  • July 20, 2021 -- Cellular reprogramming of ex vivo cells may offer improvements on first-generation adoptive cell therapies, which have experienced limited clinical success due to complex manufacturing, potential patient rejection, and availability of clinical cell types. (scienceboard.net)
  • The clinical success of anti-CD19 CAR T-cell therapies has not only provided efficacious treatment alternatives for cancer patients but also aided the developmental and regulatory precedent for the translation of cell-based therapies for other disease areas. (scienceboard.net)
  • None of the differentiation protocols proposed for clinical trials have been scrutinized over time at the single-cell level - we know they can make retinal pigment cells, but how cells evolve to that state remains a mystery," Gioele La Manno, PhD, a researcher with EPFL's Life Sciences Independent Research (ELISIR) program, noted in a news release. (ophthalmologytimes.com)
  • A pilot phase I clinical trial to assess the effects of cell therapy using autologous Epstein-Barr virus-specific cytotoxic T-lymphocytes in individuals presenting for the first time signs of multiple sclerosis is underway. (afm-telethon.fr)
Progenitor2
  • Our data suggest that, epigenetically, adult murine RPE cells are a progenitor-like cell type. (nih.gov)
  • Hafler B.P., Surzenko N., Beier K.T., Punzo C ., Trimarchi J.M., Kong J.H., Cepko C.L. (2012) Transcription factor Olig2 defines subpopulations of retinal progenitor cells biased toward specific cell fates. (umassmed.edu)
Barrier1
  • Because of a blood - retina barrier ( tight junction formation), RPE cells restrict the entry of hydrophilic macromolecules (e.g., small interfering RNA ( siRNA )) through blood stream and eye drops . (bvsalud.org)
Ganglion cell1
  • demonstrated that the absence of the ILM caused permanent retraction of the endfeet of neuroepithelial cells from the vitreal surface of the retina and the formation of a disorganized and abnormally thickened ganglion cell layer [ 3 ]. (hindawi.com)
Disease5
  • Long-term visual function is maintained in this model of retinal disease even though the xenografted cells are eventually lost, suggesting a secondary protective host cellular response. (ca.gov)
  • Individual RPE cell shape descriptors may help to delineate healthy from AMD-affected cells in early disease stages. (nevinmanimala.com)
  • Much research focuses on developing a synthetic substrate in order to transplant healthy cells to the native Bruch's membrane (BM), however, the diseased native BM and related structures still leave potential for transplanted cells to succumb to disease. (frontiersin.org)
  • Mutation of BEST1 causes retinal disease. (elifesciences.org)
  • Casual blood specimens were obtained at each examination to determine white blood cell count, serum blood urea nitrogen, glomerular filtration rate, and cystatin C. The glomerular filtration rate from serum creatinine was estimated using the Modification of Diet in Renal Disease (Study) prediction equation. (medscape.com)
Methods2
  • Methods: ARPE-19 cells were incubated with ICG (0.025-5.0 mg/ml) and with ICG-free solutions of corresponding osmolarities. (uni-tuebingen.de)
  • Standard methods such as quantitative PCR and bulk RNA-seq capture the average expression of RNAs from large populations of cells," Alex Lederer, a doctoral student at EPFL and one of the study's lead authors said in the news release. (ophthalmologytimes.com)
Protein5
  • Although its exact function is uncertain, this protein likely acts as a channel that controls the movement of negatively charged chlorine atoms (chloride ions) into or out of cells in the retina. (wikipedia.org)
  • This protein is essential for the normal function of light-sensing (photoreceptor) cells in the retina. (wikipedia.org)
  • The MITF protein helps control the development and function of pigment-producing cells called melanocytes . (medlineplus.gov)
  • Additionally, the MITF protein regulates the development of the retinal pigment epithelium. (medlineplus.gov)
  • Myosins interact with actin, a protein that is important for cell movement and shape. (medlineplus.gov)
Regenerate1
Ocular1
  • Ocular involvement-presenting as chorioretinitis, uveitis, occlusive retinal vasculitis, or optic neuritis [ 4 , 5 ]-occurs in some cases of WNV infection, although the pathogenesis and sequelae of these complications are unclear. (molvis.org)
Cystoid1
  • This was accompanied by retinal swelling and the appearance of cystoid spaces in both inner and ONLs of R91W;Nrl −/− mice indicating edema in affected areas. (nature.com)
Physiology1
  • Hence, we have identified a circadian clock mechanism in a subset of RGCs that modulates an important aspect of retinal physiology and visual processing. (jneurosci.org)