Cells repressor proteins. Medical search. Frequent questions

Researchers speculate that atrophin 1 may act as a transcriptional co-repressor. (medlineplus.gov)
A transcriptional co-repressor is a protein that interacts with other DNA-binding proteins to suppress the activity of certain genes, although it cannot attach (bind) to DNA by itself. (medlineplus.gov)
Contributions to neutropenia from PFAAP5 (N4BP2L2), a novel protein mediating transcriptional repressor cooperation between Gfi1 and neutrophil elastase. (nih.gov)
The protein encoded by this gene is a transcriptional repressor and a member of the PRDM family. (nih.gov)
PRISM/PRDM6, a transcriptional repressor that promotes the proliferative gene program in smooth muscle cells. (nih.gov)
We identified the nuclear transcriptional regulators DELLA proteins, which function as master growth repressors by inhibiting all aspects of GA responses. (duke.edu)
Transcriptional Control of Regulatory T Cells in Cancer: Toward Therapeutic Targeting? (mdpi.com)
TCF1 is inactivated by association with the transcriptional repressor TLE proteins. (biolegend.com)
During Wnt signaling, the transcriptional coactivator CTNNB1 accumulates and, in turn, replaces the transcriptional repressor associated with TCF1. (biolegend.com)
In addition to its function in thymus, TCF1 promotes T cell differentiation to Th2 cells in the periphery through transcriptional activation of GATA3. (biolegend.com)
Altogether, by identifying a pro-angiogenic VEGF/miR-101/EZH2 axis in endothelial cells we provide evidence for a functional link between growth factor-mediated signaling, post-transcriptional silencing, and histone-methylation in the angiogenesis process. (plos.org)
The identification and characterization of the transcriptional regulatory networks governing the physiology and adaptation of microbial cells is a key step in understanding their behaviour. (biomedcentral.com)
In lower multicellular fungi, the C2H2 zinc finger CreA/CRE1 protein has been shown to act as the transcriptional repressor in this process. (biomedcentral.com)
Bromodomain Protein BRD4 Is a Transcriptional Repressor of Autophagy and Lysosomal Function. (immunochemistry.com)
2) The increase in lactose concentration prompts the release of a transcriptional repressor, LacI, (3) and the subsequent expression of enhanced green fluorescent protein. (the-scientist.com)
Functions as a transcriptional repressor that suppresses cell elongation. (utoronto.ca)
HBP1, an HMG Box transcription factor, is a cell cycle inhibitor and a transcriptional repressor of the Wnt signaling pathway. (tufts.edu)
Here, we develop HT-recruit, a pooled assay where protein libraries are recruited to a reporter, and their transcriptional effects are measured by sequencing. (stanford.edu)
Fisher theorizes that copper uptake is regulated by copper-dependent transcriptional repressors and the proteins under their control. (lehigh.edu)
In differentiating neuroblastoma cells, HASH-1 is down-regulated, and there is coincident up-regulation of the transcriptional repressor HES-1, which is known to bind the HASH-1 promoter. (lu.se)
Transcription factors (TFs) are critical for B-cell differentiation, affecting gene expression both by repres- sion and transcriptional activation. (lu.se)

Nol3 has been shown to be induced in multiple cancer types and acts as a repressor of apoptosis leading to resistance and proliferation. (wikipedia.org)
Involved in negative regulation of hematopoietic stem cell differentiation and positive regulation of hematopoietic stem cell proliferation. (nih.gov)
VEGF, produced in large amounts by cancer cells during tumor growth, interacts with its receptors VEGFR1 and VEGFR2 thereby causing endothelial cell survival, proliferation, and sprouting [7] . (plos.org)
MicroRNAs control the levels of numerous cell cycle regulators that controls cell proliferation. (ukessays.com)
However, Tamoxifen Z isomer enters into the cell, competitively binding with ER to form receptor complexes, inhibiting that estrogen plays an role, and inhibiting proliferation of breast cancer cells. (lookchem.com)
Real-time cell analyzer and flow cytometry data reveal that tumor cell proliferation and apoptosis were impacted by FO. (hindawi.com)
We hypothesized that FO might regulate related genes in apoptosis and PI3K-AKT pathways to mediate the proliferation and apoptosis of liver cancer cells. (hindawi.com)
Cell proliferation and invasion were assayed using the kidney cell lines HEK293 with wild-type p53 and a ccRCC cell line MZ1257RC mutated for p53. (frontiersin.org)
HEK293 cells demonstrated a restriction of ERV-K113 env expression and invasion with no changes in proliferation in the absence of Aza. (frontiersin.org)
Major factors that may contribute to the proliferation of the leiomyoma are the decrease in the protein CCN5 and the expression of the gene HBP1--both of which are components of the Wnt signaling pathway, a fundamental pathway in normal development and in disease. (tufts.edu)
CCN5 is a matricelluar protein, which has a role in cell proliferation, angiogenesis, and progression of certain cancers. (tufts.edu)
Antagonizes TGF-beta-induced cell proliferation arrest. (hmdb.ca)
We showed that S670 dose-dependently inhibited the proliferation of human GBM cell lines U87 and U251 with IC50 values of around 6 µM. (bvsalud.org)

In Rosetta(DE3)pLacI, the rare tRNA genes are present on the same plasmid that carries the lac repressor gene. (sigmaaldrich.com)
Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. (sigmaaldrich.com)
A regulator gene may encode a protein, or it may work at the level of RNA, as in the case of genes encoding microRNAs. (wikipedia.org)
In prokaryotes, regulator genes often code for repressor proteins. (wikipedia.org)
Other regulatory genes code for activator proteins. (wikipedia.org)
Regulatory genes can also be described as positive or negative regulators, based on the environmental conditions that surround the cell. (wikipedia.org)
The binding of the lac repressor to RNA polymerase's binding site inhibits the transcription of the lac genes. (wikipedia.org)
Only when a corepressor is bound to the lac repressor will the binding site be free for RNA polymerase to carry out transcription of the lac genes. (wikipedia.org)
Knight had talked to Rettberg about the idea of applying engineering principles to biology: taking cells apart, figuring out how they work, and reassembling the parts (in this case, genes) to do something new. (technologyreview.com)
Might they one day be able to treat cells as living circuit boards, letting genes stand in for electrical components like resistors and capacitors? (technologyreview.com)
They wondered if they could ultimately redesign living cells by assembling biological "circuits" from a set of standardized "parts" (genes), just as an engineer can build circuits to control electronic devices by combining the right components. (technologyreview.com)
Assembling a given set of genes in novel ways enables synthetic biologists to accomplish highly specific and sophisticated tasks that they wouldn't be able to achieve by modifying cells one gene at a time, a process that doesn't always make it possible to control their function. (technologyreview.com)
1) The alternation of protein levels of critical oncogenes or tumour suppressor genes, which causes by miRNAs deregulation may also lead to proliferative diseases such as cancer. (ukessays.com)
Screening with RNA-Seq in Huh7 cells treated with FO revealed that 317 genes were modulated, of which 188 genes were upregulated and 129 genes were downregulated. (hindawi.com)
Herein, genome-wide transcriptome profiling (RNA-Seq) was used to screen the differentially expressed genes (DEGs) in FO-treated Huh7 cells, with the aim of uncovering the pharmacologic pattern. (hindawi.com)
Among CRE1 repressed transcripts, genes encoding unknown proteins and transport proteins were overrepresented. (biomedcentral.com)
These proteins work as activators or repressors of growth-stimulating genes. (hightimes.com)
When a repressor is present, it stops the formation of growth-stimulating genes, in parallel activators start the transcription of growth-stimulating genes. (hightimes.com)
When growth stimulating genes are activated, cells begin to grow and increase in size. (hightimes.com)
Located on the X chromosome itself, Xist produces a protein that spreads up and down the chromosome during female embryonic development, turning off its genes. (sciencedaily.com)
and the University of California, Santa Cruz, Genome Browser, to look for genes or genomic regions of chromosomes 1 and 19 thought to produce proteins that interact with Xist. (sciencedaily.com)
The researchers hunted for genes responsible for adding or subtracting so-called epigenetic marks, which attach to DNA and affect whether a cell can use a given gene. (sciencedaily.com)
The bacterial cell is able to control many protein-coding genes (green lines at bottom) with relatively few controls (yellow and purple lines). (creation.com)
1 It turns out that the bacterial genome has a few high-level instructions that control a few middle-level processes, that in turn control a massive number of protein-coding genes. (creation.com)
In the following paragraphs, we will write genes in italics and their associated proteins in Roman. (wikidoc.org)
After cloning the antibody genes into an expression vector, this is then transfected into an appropriate host cell line for antibody expression. (cellsignal.com)
DEAF-1 activates the expression of Mtk and Drs promoter-luciferase fusion genes in S2 cells. (sdbonline.org)
Transcription factor Nrf2 is a key regulator of genes encoding phase II detoxification enzymes and antioxidant proteins in response to environmental chemical insults. (cdc.gov)
Las redes funcionan para en última instancia especificar la expresión de conjuntos concretos de GENES para estados, momentos o lugares específicos. (bvsalud.org)

It plays an important role in T cell development and differentiation. (biolegend.com)
TCF1 is essential for survival of CD4+CD8+ double positive thymocytes and differentiation of T cells in the periphery. (biolegend.com)
Let-7 Let-7 has an essential role in cell cycle and the differentiation of cell cycle terminals. (ukessays.com)
In CaCo-2 colonic epithelial cells, the expression of this gene increases with cell differentiation. (aacrjournals.org)
All-trans retinoic acid (RA) is the most active metabolite of vitamin A. Several studies have described a pivotal role for RA signalling in different biological processes such as cell growth and differentiation, embryonic development and organogenesis. (mdpi.com)
Caulobacter crescentus is an aquatic free-living Gram-negative bacterium whose cell cycle depends on cell differentiation. (usp.br)
Modulation of Basic Helix-Loop-Helix Transcription Complex Formation by Id Proteins during Neuronal Differentiation. (lu.se)
We found that the three Id proteins expressed in neuroblastoma cells (Id1, Id2, and Id3) were down-regulated during induced differentiation, indicating that Id proteins help keep the tumor cells in an undifferentiated state. (lu.se)
The ability of Id proteins to affect HES-1 activity is of particular interest in neuronal cells, where regulation of HES-1 is essential for the timing of neuronal differentiation. (lu.se)
In this study, highly purified, flow-cytometry sorted, classified in relation to normal B-cell differentiation [1]. (lu.se)
Interacting DNA-encoded regulatory subsystems in the GENOME that coordinate input from activator and repressor TRANSCRIPTION FACTORS during development, cell differentiation, or in response to environmental cues. (bvsalud.org)

The bHLH protein SCL/Tal-l interacts with the co-repressor ETO-2 in erythroid cells and megakaryocytes. (ox.ac.uk)
The extended CAG region changes the structure of atrophin 1 and how the protein interacts with other proteins to control gene function. (medlineplus.gov)
Surprisingly, we recently found that DELLA directly interacts with multiple classes of key regulatory proteins in other signaling pathways. (duke.edu)
Pan D, Estevez-Salmeron LD, Stroschein SL, Zhu X, He J, Zhou S, Luo K: The integral inner nuclear membrane protein MAN1 physically interacts with the R-Smad proteins to repress signaling by the transforming growth factor-{beta} superfamily of cytokines. (hmdb.ca)

An example of a regulator gene is a gene that codes for a repressor protein that inhibits the activity of an operator (a gene which binds repressor proteins thus inhibiting the translation of RNA to protein via RNA polymerase). (wikipedia.org)
In terms of the lac operon, the negative regulator would be the lac repressor which binds to the promoter in the same site that RNA polymerase normally binds. (wikipedia.org)
If the estrogen receptor (ER) is present in breast cancer cells, estrogen enters into tumor cells and binds with ER, promoting mRNA and DNA synthesis of tumor cells, stimulating tumor cell growth. (lookchem.com)
Lin F, Morrison JM, Wu W, Worman HJ: MAN1, an integral protein of the inner nuclear membrane, binds Smad2 and Smad3 and antagonizes transforming growth factor-beta signaling. (hmdb.ca)
Bacteriophage Lambda binds to the target E. coli cell, the tail tip binding to a maltose receptor. (wikidoc.org)
In addition, the expression of these fusions are no longer cell-cycle regulated, as well as GroEL synthesis in a strain which does not have the HrcA protein, the putative repressor that binds CIRCE, indicating that the CIRCE-HrcA system are involved in cell cycle regulation of groESL in C. crescentus. (usp.br)
The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein is a candidate vaccine antigen that binds angiotensin-converting enzyme 2(ACE2), leading to virus entry. (cdc.gov)

The general dogma is that these regulatory elements get activated by the binding of transcription factors, proteins that bind to specific DNA sequences, and control mRNA transcription. (wikipedia.org)
Each time an amino acid is added to a growing polypeptide during protein synthesis, a tRNA anticodon pairs with its complementary codon on the mRNA molecule, ensuring that the appropriate amino acid is inserted into the polypeptide. (genome.gov)
In a cell, antisense DNA serves as the template for producing messenger RNA (mRNA), which directs the synthesis of a protein. (genome.gov)
The binding of miRNA- protein complexes to mRNA causes translation inhibition or destabilisation of target transcripts. (ukessays.com)
1) This is resulting in the downregulation of the protein encoded by mRNA. (ukessays.com)
In rats, hepatic ketogenic capacity increases rapidly during postnatal development ( 4 ) or fasting ( 5 ) when the mRNA, protein, and activity of HMGCS2 increase in liver ( 6 - 8 ). (aacrjournals.org)
Administration of dibutyryl-cyclic AMP to adult rats increases mRNA, protein levels, and enzymatic activity of HMGCS2 in the liver ( 6, 9 ). (aacrjournals.org)
The maternally expressed Pat1a protein acts as a translational repressor, while Pat1b functions in mRNA decay in somatic cells. (embl.de)
In MEF cells with wild type Nrf2 and mouse hepa 1c1c7 cells, Cd increased the Nrf2 protein level but not the Nrf2 mRNA level dose-dependently. (cdc.gov)

Once activated, p53 coordinates multiple downstream pathways, thereby maintaining the homeostasis of the host cell or organism (if the stress is mild, transient, and repairable) or eliminating damaged cells (if the stress is acute, prolonged, and difficult to resist). (nature.com)
In this review, we summarize the current knowledge on the different intracellular pathways involved in the programming of Treg cell homeostasis and functions in cancer. (mdpi.com)
These pathways are comprised of many different enzymes that collaborate to deliver signals from the environment into the cell. (lehigh.edu)
All of the proteins and pathways that we're studying are used by this species, even though versions of them are also employed by many other bacterial species as well. (lehigh.edu)

In prokaryotes, a well-known activator protein is the catabolite activator protein (CAP), involved in positive control of the lac operon. (wikipedia.org)
The simplest interpretation of these results is that Dri can act either as an activator or as a repressor, depending on the context within which it finds itself. (sdbonline.org)

Members of this family have been shown to bind to KNOX and BELL- like TALE class homeodomain proteins. (utoronto.ca)

In the regulation of gene expression, studied in evolutionary developmental biology (evo-devo), both activators and repressors play important roles. (wikipedia.org)
Essentially, activators and repressors act like the stop and start pedals on a car. (hightimes.com)

however, restoration of CBY1 expression in tumor cells reduces BCSC and its enrichment, thus lnc408 plays an essential role in maintenance of BCSC stemness. (nature.com)
GEP analyses were performed on highly purified, flow-cytometry sorted tumor-cells from eight subgroups of BCLs. (lu.se)
This enabled identification of TFs that can be uniquely associated to the tumor cells of chronic lymphocytic leukemia (CLL), diffuse large B-cell lymphoma (DLBCL), follicular lymphoma (FL), hairy cell leukemia (HCL), and mantle cell lymphoma (MCL). (lu.se)

All of it fits into the cell nucleus. (creation.com)
Using cell fractionation and immunoprecipitation, we further demonstrated that Nrf2 and Keap1 were both detected in the cytoplasm and nucleus. (cdc.gov)

Cd did not affect the protein levels of Keap1, which is a repressor of the cytoplasmic Nrf2, or Cul-3, an E3 ligase involved in the proteasomal degradation of Nrf2. (cdc.gov)

Knockout cell lysate achieved by CRISPR/Cas9. (abcam.com)
Access thousands of knockout cell lysates, generated from commonly used cancer cell lines. (abcam.com)
See here for more information on knockout cell lysates. (abcam.com)
Genetic experiments using mouse embryonic fibroblast (MEF) cells derived from wild type or Nrf2 knockout mice revealed that the cells lacking Nrf2 exhibited markedly increased production of ROS and sensitivity to Cd cytotoxicity. (cdc.gov)

It is assumed that the Id helix-loop-helix (HLH) proteins act by associating with ubiquitously expressed basic HLH (bHLH) transcription factors, such as E47 and E2-2, which prevents these factors from forming functional hetero- or homodimeric DNA binding complexes. (lu.se)
Several tissue-specific bHLH proteins, including HASH-1, dHAND, and HES-1, are important for development of the nervous system. (lu.se)

Scope includes mutations and abnormal protein expression. (cancerindex.org)
DPR and NFJS are considered to be allelic disorders, attributable to mutations on the nonhelical (E1/V1) head domain in the KRT14 gene, with consequent premature termination of protein synthesis. (medscape.com)

The 32-aa MYND domain (for myeloid, Nervy, and Deaf-1) contains non-DNA-binding zinc fingers that are thought to mediate protein-protein interactions (Gross, 1996). (sdbonline.org)

The encoded protein is involved in regulation of vascular smooth muscle cells (VSMC) contractile proteins. (nih.gov)

Cell signaling in plant development usually involves a receptor (i.e. for a hormone or light molecule) and a signal transduction pathway, which concludes with a cellular response that is relevant to the plants development. (hightimes.com)
In response to the signal transduction pathway (initiated by the presence of plant hormones or PGRs), repressor proteins for example are then broken down and the "stop breaks" are removed. (hightimes.com)

Although the exact function of this protein is unknown, it appears to play an important role in nerve cells (neurons) in many areas of the brain. (medlineplus.gov)
This altered protein accumulates in neurons and interferes with normal cell functions. (medlineplus.gov)
Neural defects of retn mutant cells include mushroom body ß-lobe fusion and pathfinding errors by photoreceptor and subesophageal neurons. (sdbonline.org)
Ewing sarcomas are thought to derive from cells of the neural crest, possibly mesenchymal stem cells, via a pathway that might include postganglionic cholinergic neurons. (medscape.com)

Functionally, 'TGF-beta-converted' T(reg) cells generated from TIEG1-deficient mice were unable to suppress airway inflammation in vivo. (nih.gov)
Our technical expertise include lentiviral vectors, transgenic mice and stem cells cultures. (lu.se)
To demonstrate the flexibility and modular nature of the liposome system, 10 recombinant surface antigens representing distinct influenza virus strains were bound simultaneously to generate a highly multivalent protein particle that with 5 ng individual antigen dosing induced antibodies in mice that specifically recognized the constituent immunogens and conferred protection against heterologous H5N1 influenza virus challenge. (cdc.gov)
Compared to other vaccine formulations, immunization using CoPoP liposomes admixed with recombinant RBD induces multiple orders of magnitude higher levels of antibody titers in mice that neutralize pseudovirus cell entry, block RBD interaction with ACE2, and inhibit live virus replication. (cdc.gov)

The ATN1 gene variants that cause CHEDDA syndrome occur in one of the two copies of the gene in each cell and lead to a change in single protein building blocks (amino acids) in atrophin 1. (medlineplus.gov)
A protein consists of one or more chains of amino acids (called polypeptides) whose sequence is encoded in a gene. (genome.gov)
By tiling 238 proteins, we find repressors as short as ten amino acids. (stanford.edu)

Cell expansion is primarily driven by water uptake into the cells cytoplasm, which accumulates in the cells central vacuole. (hightimes.com)

Protein interactions of the transcription factor Hoxa1. (nih.gov)
DELLA proteins play a central role in these processes via direct protein-protein interactions with key transcription factors. (duke.edu)
Mayya V, Lundgren DH, Hwang SI, Rezaul K, Wu L, Eng JK, Rodionov V, Han DK: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. (hmdb.ca)
Studying interactions, we noted that all four Id proteins could dimerize with E47 or E2-2, but not with HASH-1 or dHAND. (lu.se)
water-mediated interactions resulted in severely [email protected] reduced af®nities for trp repressor (Joachimi ak et al. (lu.se)
Since macromolecules comprise 20-40% of the volume of a cell, one would expect that steric interactions dominate macromolecular diffusion. (lu.se)

1 Division of Cell Biology, La Jolla Institute for Allergy and Immunology, La Jolla, California 92037, USA. (nih.gov)

The most likely explanation for this phenomenon, Migeon reasons, was that a protein that represses the X chromosome silencing activity of Xist was working overtime, allowing more than one X chromosome to remain activated. (sciencedaily.com)
To identify the likely location of the repressor protein and the gene that codes for it, the researchers started by looking at cells from human embryos with different forms of chromosomal trisomy, a condition in which cells carry three copies of a particular chromosome instead of two. (sciencedaily.com)
Once the phage has injected its DNA into its host , the phage DNA may integrate itself into the host cell chromosome. (wikidoc.org)

Novagen Rosetta strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. (sigmaaldrich.com)
Expression host strains for pETBlue and pTriEx vectors are lDE3 lysogens that also carry the compatible pLacI plasmid, encoding the lac repressor. (sigmaaldrich.com)
Protein expression and function of organic anion transporters in short-term and long-term cultures of Huh7 human hepatoma cells. (cancerindex.org)
Transforming growth factor-beta (TGF-beta) signaling in naive T cells induces expression of the transcription factor Foxp3, a 'master' regulator of regulatory T cells (T(reg) cells). (nih.gov)
Here we show that Itch-/- T cells were resistant to TGF-beta treatment and had less Foxp3 expression. (nih.gov)
TMA results showed the highest amount of ERV-K env protein expression and the strongest significant membrane expression in ccRCC versus other RCC subtypes. (frontiersin.org)
High ERV-K env total protein expression of all tumor subtypes significantly correlated with low tumor grading and a longer disease specific survival using multivariable analyses. (frontiersin.org)
HMGCS2 protein expression is down-regulated preferentially in moderately and poorly differentiated carcinomas. (aacrjournals.org)
The repressor found in the phage lambda is a notable example of the level of control possible over gene expression by a very simple system. (wikidoc.org)
The expression of the groESL operon is induced by heat shock and is cell cycle controlled at normal temperatures, with maximal transcription in the predivisional cell and very low levels in the stalked cell. (usp.br)

The pLacI plasmid confers chloramphenicol resistance and provides enough lac repressor to inhibit transcription from the T7 lac promoter in the absence of inducer. (sigmaaldrich.com)
N- trans -Feruloyloctopamine (FO), a natural compound, was reported in our previous study to inhibit a tumor cell malignant phenotype by AKT- and EMT-related signals and might be used as a promising drug for HCC treatment. (hindawi.com)

Examples such as cFLIP suppress cell death mechanisms leading to pathological disorders like cancer, and thus play a crucial role in drug resistance. (wikipedia.org)

They are usually constantly expressed so the cell always has a supply of repressor molecules on hand. (wikipedia.org)
We anticipate that the versatility of SPOTlight will facilitate its deployment to decipher the rules of life, understand diseases, and engineer new molecules and cells. (stanford.edu)

Moreover, we found that glioblastoma cells, notorious for their VEGF production, elicited a similar response when co-cultured with endothelial cells [11] . (plos.org)
Uterine leiomyoma is a collection of hyperplastic uterine smooth muscle, along with endothelial cells, fibroblast, collagen, and sulfate proteoglycan. (tufts.edu)

Although the conventional activities of p53 such as cell cycle arrest, senescence, and apoptosis are well accepted as the major checkpoints in stress responses, accumulating evidence implicates the importance of other tumor suppression mechanisms. (nature.com)
Is p53-dependent ferroptosis sufficient for tumor suppression in the absence of cell cycle arrest, senescence, and apoptosis? (nature.com)
To date, various mechanisms have been suggested to explain the powerful tumor-suppressive effect of p53, including the induction of cell cycle arrest, senescence, and apoptosis. (nature.com)
Deletion of TCF1 causes massive apoptosis of double positive thymocyte, suggesting that TCF1 is required for thymocyte survival during T cell development. (biolegend.com)
KEGG enrichment analysis showed that FO mainly regulates PI3K-AKT- and apoptosis-related signals, in which BBC3, DDIT3, NOXA, and CDKN1A on the surface serve as the novel targets of FO inducing HCC cell apoptosis. (hindawi.com)
The result implied that FO might exacerbate HCC cell apoptosis by regulating BBC3, DDIT3, CDKN1A, and NOXA signals. (hindawi.com)
N- trans -Feruloyloctopamine (FO) is the extract of garlic skin, which possesses high antioxidant activity and selectively induces cell apoptosis in leukemia tumors [ 6 ]. (hindawi.com)

We find a relationship between repressor function and evolutionary age for the KRAB domains, discover that Homeodomain repressor strength is collinear with Hox genetic organization, and identify activities for several domains of unknown function. (stanford.edu)

It controls the interaction network of hundreds of thousands of proteins. (creation.com)
Can function as a specific repressor of TGF-beta, activin, and BMP signaling through its interaction with the R-SMAD proteins. (hmdb.ca)

A leading contributing factor to metastasis and treatment resistance is the heterogeneity and plasticity of the cells within tumors 6 . (nature.com)
Among immune cells, Foxp3 + regulatory T cells (Treg cells) are potent inhibitors of cancer immunity, and their presence within solid tumors is generally associated with a poor prognosis. (mdpi.com)
B-cell lymphomas (BCLs) constitute a diverse set of tially identify new functional, diagnostic, and therapeutic tumors, both morphologically and clinically, that are mainly targets. (lu.se)

In vitro , the sprouting and migratory properties of primary endothelial cell cultures were reduced by inhibiting EZH2 through up-regulation of miR-101, siRNA-mediated knockdown of EZH2, or treatment with 3-Deazaneplanocin-A (DZNep), a small molecule inhibitor of EZH2 methyltransferase activity. (plos.org)
1) The regulation of these protein levels is vital for the understanding of the cell cycle control and its dysregulation. (ukessays.com)
1) For example, the down-regulation of miR15s and miR - 16 - 1 in chronic lymphocytic leukaemia, prostate cancer and pituitary tumours, leading to the inhibition of tumour growth and induce cell cycle arrest at the G 1 - phase by target cell cycle regulators ( cyclin D1, cyclin E1, cyclin D3 and CDK6). (ukessays.com)
To understand the role of the epigenetic co-repressor protein TRIM28 in the control of transposable elements (TEs) and how TEs control gene regulation in the developing and adult brain. (lu.se)

Sickle cell anemia is an example of an autosomal recessive genetic disorder. (genome.gov)
Synthetic biology goes further than genetic engineering, which usually involves adding a single gene to a cell so that it will do something it wouldn't normally do. (technologyreview.com)
It is also different from metabolic engineering, which uses the techniques of genetic engineering to maximize cells' production of commercially useful products, such as insulin. (technologyreview.com)
Unraveling the genetic and epigenetic determinants of phenotypes is critical for understanding and re-engineering biology and would benefit from improved methods to separate cells based on phenotypes. (stanford.edu)
However, the field lacks an accessible method to screen and sort droplets based on cellular phenotype upstream of genetic analysis, particularly for large and complex cells. (stanford.edu)
Traditional polyclonal and monoclonal antibodies are the product of normal B cell development and genetic recombination. (cellsignal.com)

Mammalian cell lines are most commonly used for recombinant antibody production, although cell lines of bacterial, yeast, or insect origin are also suitable. (cellsignal.com)
She and her team are honing in on studying the structures of some of these proteins and how they interact with copper ions to learn more about how they function within bacterial cells. (lehigh.edu)

TCF1 is the first identified member of the T-cell-specific transcription factor family. (biolegend.com)

We have cloned previously the human long-chain fatty acid-CoA ligase 4 (FACL4), which is a central enzyme in controlling the free arachidonic acid level in cells and thereby regulating eicosanoid production. (nih.gov)
Human-derived hepatic cell lines are a valuable alternative to primary hepatocytes for drug metabolism, transport and toxicity studies. (cancerindex.org)
Accordingly, immunohistochemistry with specific antibodies shows that HMGCS2 is expressed mainly in differentiated cells of human colonic epithelium. (aacrjournals.org)
However, she says, the gene responsible for this repressor, or even its approximate location in the human genome, has been unclear. (sciencedaily.com)
Scholars@Duke publication: Human papillomavirus type 16 infection and squamous cell carcinoma of the head and neck in never-smokers: a matched pair analysis. (duke.edu)
PURPOSE: Infection with human papillomavirus (HPV) type 16 has been suggested to be a risk factor for squamous cell carcinoma of the head and neck (SCCHN) and to be more commonly associated with SCCHN occurring in the oropharynx and in never-smokers. (duke.edu)
Here, we report SPOTlight, a versatile high-throughput technique to isolate individual yeast or human cells with unique spatiotemporal profiles from heterogeneous populations. (stanford.edu)
These results provide a resource of 600 human proteins containing effectors and demonstrate a scalable strategy for assigning functions to protein domains. (stanford.edu)
To understand the functional analyses of miRNAs and their role in neural stem cells by characterizing and identifiying known and novel RNAs bound to the mouse and human RNA induced Silencing Complex in various cell types of the brain. (lu.se)

Contains the pLacI plasmid producing extra Lac repressor. (sigmaaldrich.com)
In our project, we aim at creating a cell-to-cell communication system that allows the propagation of a set of instructions coded on a plasmid, and not just binary information as in quorum sensing. (igem.org)

Using affinity chromatography coupled to multidimensional protein identification technology (MudPIT), DEAF-1 was identified as a candidate regulator. (sdbonline.org)

Mitochondrial carriers catalyse the translocation of numerous metabolites across the inner mitochondrial membrane, playing a key role in different cell functions. (cancerindex.org)
We uncovered the functions of ABCE proteins Rli1/ABCE1 and Arb1 in PIC assembly and ribosome biogenesis, and identified the tRNA methyltransferase Gcd10/Gcd14, which contributed to the discovery of the TRAMP-mediated RNA surveillance pathway. (nih.gov)

One such wide-domain regulatory circuit, essential to all cells, is carbon catabolite repression (CCR): it allows the cell to prefer some carbon sources, whose assimilation is of high nutritional value, over less profitable ones. (biomedcentral.com)

Nucleolar protein 3 is a protein that in humans is encoded by the NOL3 gene. (wikipedia.org)
In each cell, most humans have 23 pairs of chromosomes, for a total of 46. (sciencedaily.com)

HCC cell lines were maintained in high glucose DMEM (Invitrogen, California, USA) supplemented with 10% fetal bovine serum (FBS) (Life Technologies) and 1% (100 U/mL) penicillin-streptomycin (Life Technologies), then sustained at 37°C in a humidified incubator at 5% CO 2 . (hindawi.com)

Transfecting these cell lines with a codon optimized ERV-K113 env overexpressing CMV vector was performed with or without 5'-Aza-2'-deoxycytidine (Aza) treatment to sustain promoter de-methylation. (frontiersin.org)

Repressor proteins bind to operators or promoters, preventing RNA polymerase from transcribing RNA. (wikipedia.org)
Inducers cause repressor proteins to change shape or otherwise become unable to bind DNA, allowing RNA polymerase to continue transcription. (wikipedia.org)
In addition, several other proteins, called transcription cofactors, bind to the transcription factors themselves to control transcription. (wikipedia.org)
Both Int and IHF bind to attP and form an intasome , a DNA-protein-complex designed for site-specific recombination of the phage and host DNA. (wikidoc.org)

They can either modify an existing single-cell organism-for example, a bacterium-or start from scratch and build a membrane-encapsulated protocell. (the-scientist.com)
Existing protocell designs have consisted of a simple lipid membrane separating the inside of the cell and the reactions therein from the outside environment. (the-scientist.com)
When all resources of the host are depleted from building new phages, the host cell is lysed (the cell membrane is broken down), and the new phages are released. (wikidoc.org)

Mol Cell Biol, 2006 Apr. (nih.gov)

We demonstrate the capabilities of this method by encapsulating five standardized mammalian cell lines of varying sizes and morphologies as well as a heterogeneous cell mixture of a whole dissociated flatworm (5-25 mum in diameter) within highly monodisperse double emulsions (35 mum in diameter). (stanford.edu)

As a result, the protein is altered and cannot function normally, though it is unclear how these changes lead to the specific features of CHEDDA syndrome. (medlineplus.gov)
Research has intensified to understand ERV protein function and their role as tumor antigens and targets for cancer (immune) therapy. (frontiersin.org)
It is posited that some of these retn -expressing cells function in females to repress a male behavioral pathway activated in males by fru M ( Ditch, 2005 ). (sdbonline.org)
In the past five years, droplet microfluidic techniques have unlocked new opportunities for the high-throughput genome-wide analysis of single cells, transforming our understanding of cellular diversity and function. (stanford.edu)

The uptake and storage system consists of a metal transporter and metallothioneins (metal binding proteins). (igem.org)

Our previous report showed that FO inhibits HCC cell invasion via AKT-, mitogen-activated protein kinase- (MAPK-), and EMT-related signals [ 7 ]. (hindawi.com)
With the presence of both signals, the AND gate outputs a repressor protein and then changes the state of the bistable switch, which acts as a memory module. (igem.org)
In this way, our E. coli cells can convert the information about the concurrence of the two signals into its memory. (igem.org)

In MLL leukemia, LAMP5-AS1 facilitates the methyltransferase activity of DOT1L to drive leukemia cell stemness 18 . (nature.com)
T he presence and activity of various proteins are required in the cell cycle progression. (ukessays.com)
To measure lysosomal Cathepsin B activity, cells were incubated with Magic Red CathepsinB (ImmunoChemistry Technologies, Cat#: 938) for 1 hr according to the manufacturer's instructions. (immunochemistry.com)
Enzymatic activity assay Cathepsin L activity in living cells was monitored using the Magic Red Cathepsin L detection kit (ImmunoChemistry Technologies, MN, USA). (immunochemistry.com)
In cells with abundant nutrients, protease activity is high, which breaks down cII. (wikidoc.org)
In cells with limited nutrients, protease activity is low, making cII stable. (wikidoc.org)
However, the Id proteins did complex with HES-1, and increased levels of Id2 reduced the DNA binding activity of HES-1. (lu.se)

TC abundance is reduced in starved cells by phosphorylation of eIF2α by Gcn2, converting eIF2 from substrate to inhibitor of its guanine nucleotide exchange factor (GEF) eIF2B. (nih.gov)

retn is implicated as a major repressor of male courtship behavior in the absence of the fruitless ( fru ) male protein. (sdbonline.org)
In the absence of cI proteins, the cro gene may be transcribed. (wikidoc.org)

If so, they could treat biology as a manufacturing technology, programming cells to produce things they wouldn't normally make-for example, drugs, fuels, or plastics. (technologyreview.com)
Cell-to-cell communication systems are important because, in most synthetic biology applications, the desired tasks are generally accomplished by a population of cells, rather than by a single cell. (igem.org)

Males mutant for retn court with normal parameters, although feminization of retn cells in males induces bisexuality. (sdbonline.org)

The initiation and development of tumor cell is accompanied by elevated stresses, such as oncogene activation, DNA damage, genome instability, and reprogrammed tumor metabolism. (nature.com)

We are engineering our E. coli cells to process the correlation information of two enviornmental signal, similar to the process of conditioning in higher orgamisms. (igem.org)
After the memory module is switched and given the "conditioned stimulus", the E. coli cells will pass the information to the reporter module and thus exhibit the "conditioned response. (igem.org)

Based on these findings, we propose that ketogenesis is an undesirable metabolic characteristic of the proliferating cell, which is down-regulated through c-Myc-mediated repression of the key metabolic gene HMGCS2 . (aacrjournals.org)

Nevertheless, the stage is set for the molecular simulations of ever more complex subcellular processes and we discuss one such case, the diffusion of lac repressor in a packed cellular nucleoid. (lu.se)

Anatomy7

Organisms8

Chemicals and Drugs50

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes61

Information Science3

Transcriptional21

Proliferation13

Genes29

Differentiation11

Interacts4

Binds7

MRNA9

Pathways4

Activator2

Homeodomain proteins1

Activators2

Tumor cells3

Nucleus2

Cytoplasmic1

Knockout4

BHLH2

Mutations2

Mediate1

Vascular smooth muscl1

Signal Transduction2

Neurons4

Mice4

Amino acids3

Accumulates1

Interactions6

Immunology1

Chromosome3

Expression10

Inhibit2

Suppress1

Molecules2

Endothelial cells2

Apoptosis7

Evolutionary1

Interaction2

Tumors3

Regulation4

Genetic6

Bacterial2

Transcription factor1

Human9

Plasmid2

Regulator1

Functions2

Regulatory1

Humans2

Glucose1

Promoter1

Bind4

Membrane3

Biol1

Mammalian1

Function4

Consists1

Signals3

Activity7

Phosphorylation1

Absence2

Biology2

Induces1

Metabolism1

Coli2

Repression1

Cellular1