• There is a longstanding belief that a given nucleus controls a defined volume of cytoplasm, so when a muscle grows (hypertrophy) or shrinks (atrophy), the number of myonuclei change accordingly. (frontiersin.org)
  • Recently, two independent models, one from rodents and the other from insects, have demonstrated that nuclei are not lost from skeletal muscle fibers when they undergo either atrophy or programmed cell death. (frontiersin.org)
  • We previously reported that local administration of bFGF 1 month after recurrent laryngeal nerve (RLN) paralysis compensated for atrophy of the thyroarytenoid muscle. (biomedcentral.com)
  • As increased neuromuscular junctions are expected to maintain myofiber volume, bFGF administration may prevent thyroarytenoid muscle atrophy in the mid to long term. (biomedcentral.com)
  • These treatments provide only static adjustment to the larynx, and their effects may be limited by the remaining hoarseness due to progressive atrophy of the paralyzed thyroarytenoid (TA) muscle. (biomedcentral.com)
  • Therefore, medications that can prevent the progression of muscle atrophy or promote muscle regeneration are in high demand. (biomedcentral.com)
  • Acupuncture is effective for the alleviation of denervated skeletal muscle atrophy. (acupunctureofiowa.com)
  • The results of the aforementioned research indicates that acupuncture is an effective treatment modality for skeletal muscle atrophy. (acupunctureofiowa.com)
  • Effect of electroacupuncture on denervated skeletal muscle atrophy and expression of IGF-1/PI3K/AKT in rats [J]. Chinese Acupuncture and Moxibustion, 2018,38(12):1311-1317. (acupunctureofiowa.com)
  • Experimental study of rat denervated skeletal muscle atrophy [J]. Journal of Shanghai Medical University, 1999, 26(4): 265-268. (acupunctureofiowa.com)
  • This assumption long seemed valid, with many researchers reporting the presence of disintegrating nuclei in muscle tissue during atrophy induced by inactivity, injury or paralysis. (robert-gorter.info)
  • Muscles get damaged during extreme exercise, and often have to weather changes in food availability and other environmental factors that lead to atrophy. (robert-gorter.info)
  • Consistently, p53-null mice are prone to cancer improvement but resistant to cancer-induced muscle atrophy [74]. (lckinhibitor.com)
  • Similarly, in doxorubicin-induced muscle atrophy p53 exerts its effect by means of PW1 [74]. (lckinhibitor.com)
  • Loss of muscle innervation activates the Hippo pathway along with the inhibition of MST1 is sufficient to stop atrophy in denervated, fast-twitch muscles [79]. (lckinhibitor.com)
  • Conversely, but in parallel, denervation increases YAP protein amount and activity in myonuclei, as a compensatory pro-trophic signal to attenuate muscle atrophy improvement [80]. (lckinhibitor.com)
  • YAP/TAZ positively regulate satellite cell/myoblast activation, and we tentatively speculate that dysfunctions within this pathway may possibly play a relevant role in muscle atrophy development,Cells 2021, 10,6 ofespecially in sarcopenia, where lowered recruitment of satellite cells seems to become mechanistically involved in loss of muscle mass [81]. (lckinhibitor.com)
  • Oxidative and Nitrosative Anxiety Oxidative tension, together with nitrosative tension, represents a significant player of muscle atrophy development. (lckinhibitor.com)
  • On the other hand, the upregulation of chaperones and enzymes involved inside the anti-oxidant defense happens prior to muscle atrophy improvement, supporting the hypothesis that the enhance in oxidant production anticipates the raise in protein catabolism [86,87]. (lckinhibitor.com)
  • Obestatin signalling counteracts glucocorticoid-induced skeletal muscle atrophy via NEDD4/KLF15 axis. (institut-myologie.org)
  • More specifically, my laboratory investigates the role inflammation plays in muscle atrophy, as well as during recovery and regeneration after injury. (uncg.edu)
  • In lung diseases, muscle mass usually decreases and severe atrophy occurs. (jeoct.com)
  • Since myostatin and follistatin bind to the same receptor, the follistatin to myostatin (F:M) ratio is associated with muscle atrophy or hypertrophy [ 12 ]. (biomedcentral.com)
  • It is a protein with a key role in regulating muscle differentiation or myogenesis, specifically the development of skeletal muscle. (wikipedia.org)
  • In zebrafish, Myf5 is the first MRF expressed in embryonic myogenesis and is required for adult viability, even though larval muscle forms normally. (wikipedia.org)
  • As no muscle is formed in Myf5;Myod double mutant zebrafish, Myf5 cooperates with Myod to promote myogenesis. (wikipedia.org)
  • Cell culture experiments indicated that Gln could promote cell proliferation in a dose dependent manner, but expression of myogenesis regulatory genes was not altered. (nature.com)
  • Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD. (chinagene.cn)
  • MicroRNA-1/206(miRNA-1/206) is specifically expressed in skeletal muscle and play a critical role in myogenesis. (biomedcentral.com)
  • miR-101a promotes the differentiation of goat skeletal muscle satellite cells (SMSCs), as we previously reported, but the underpinning mechanism remains to be illuminated. (chinagene.cn)
  • miR-101a targeting EZH2 promotes the differentiation of goat skeletal muscle satellite cells[J]. Hereditas(Beijing), 2017, 39(9): 828-836. (chinagene.cn)
  • Methods: Goat skeletal muscle satellite cells isolated in the pre-laboratory were used as the test material. (koreascience.kr)
  • In addition, miR-188-5p was transfected into goat skeletal muscle satellite cells by constructing mimics and inhibitors of miR-188-5p, respectively. (koreascience.kr)
  • After several rounds of proliferation, most SCs differentiate into myoblasts, which fuse to form new myofibers [ 5 ]. (biomedcentral.com)
  • We previously reported that local injection of single, high-dose bFGF into the paralyzed vocal fold 1 month after RLN transection compensated for an atrophied TA muscle by inducing activation of SC proliferation and increasing the number of myoblasts in the RLN paralysis model of rats [ 12 ]. (biomedcentral.com)
  • Despite only being present for a few days during embryonic development, Myf5 is still expressed in certain adult cells. (wikipedia.org)
  • Gros J, Manceau M, Thomé V, Marcelle C. A common somitic origin for embryonic muscle progenitors and satellite cells. (scielo.br)
  • The expression level of the SFRP1 was significantly higher in the embryonic skeletal compared with postnatal skeletal muscle, whereas miR-206 showed the inverse pattern of expression. (biomedcentral.com)
  • Embryonic myogenic progenitor cells express CXCR4, G-protein coupled cell surface receptor, and migrate towards regions of SDF-1 expression during limb muscle development, suggesting that CXCR4/SDF-1 plays a role in muscle cell homing. (biomedcentral.com)
  • A new tumor model, combining human embryonic stem cells (ESC) and tumor cells, develops abundant human vessels. (nih.gov)
  • As one of the key cell markers of satellite cells (the stem cell pool for skeletal muscles), it plays an important role in the regeneration of adult muscle. (wikipedia.org)
  • Cellular and molecular regulation of muscle regeneration. (scielo.br)
  • Satellite cells are essential for skeletal muscle regeneration:the cell on the edge returns centre stage. (scielo.br)
  • Skeletal muscle regeneration is regulated by coordinated activation of multiple signaling pathways. (elifesciences.org)
  • However, the role of individual arms of the UPR in skeletal muscle regeneration remain less understood. (elifesciences.org)
  • Myofiber-specific ablation of IRE1α or XBP1 in mice diminishes skeletal muscle regeneration that is accompanied with reduced number of satellite cells. (elifesciences.org)
  • Finally, targeted ablation of IRE1α also reduces Notch signaling, abundance of satellite cells, and skeletal muscle regeneration in the mdx mice, a model of Duchenne muscular dystrophy. (elifesciences.org)
  • Collectively, our experiments suggest that the IRE1α-mediated signaling promotes muscle regeneration through augmenting the proliferation of satellite cells in a cell non-autonomous manner. (elifesciences.org)
  • The findings shed new light on mechanisms of regeneration of healthy myofibers after severe tissue injury and suggest interplay between these fibers and muscle satellite cells though mechanisms remain to be elucidated. (elifesciences.org)
  • However, the mechanisms of muscle regeneration are not yet fully understood. (elifesciences.org)
  • Considering that reinnervation of the TA muscle can occur even after UVFP through these anastomoses, it would be ideal if drugs that promote nerve regeneration could be used to treat UVFP. (biomedcentral.com)
  • Satellite cells (SCs), which are precursors of skeletal muscles, such as the TA muscle, play a key role in muscle homeostasis and regeneration. (biomedcentral.com)
  • SCs are normally in a quiescent state in adult muscles, and start proliferating during muscle regeneration in response to denervation. (biomedcentral.com)
  • Low-intensity pulsed ultrasound (LIPUS, a form of mechanical stimulation) can promote skeletal muscle functional repair, but a lack of mechanistic understanding of its relationship and tissue regeneration limits progress in this field. (ijbs.com)
  • We investigated the hypothesis that specific energy levels of LIPUS mediates skeletal muscle regeneration by modulating the inflammatory microenvironment. (ijbs.com)
  • LIPUS at intensity of 60mW/cm 2 could significantly promoted skeletal muscle regeneration through shifting macrophage phenotype from M1 to M2. (ijbs.com)
  • Our team is working on the molecular and cellular actors involved in human muscle regeneration , in muscle ageing and in muscular dystrophies including oculopharyngeal muscular dystrophy (OPMD) and Duchenne muscular dystrophy (DMD). (institut-myologie.org)
  • More precisely we are working on RNA metabolism , muscle regeneration , muscle stem cells , and fibrosis , with the final aim of developing innovative therapeutic approaches. (institut-myologie.org)
  • Additionally, we are interested in mechanisms responsible for skeletal muscle recovery and regeneration after injury. (uncg.edu)
  • Aged skeletal muscle displays increased fibrosis and impaired regeneration. (uncg.edu)
  • In aged skeletal muscle, impairments in regrowth and regeneration may be explained by a decreased responsiveness of muscle precursor cells (MPCs) to environmental cues such as growth factors. (uncg.edu)
  • Cell Metabolism 2013;17(2):210-24. (scielo.br)
  • Medium pH modulates matrix, mineral, and energy metabolism in cultured chick bones and osteoblast-like cells. (sciepub.com)
  • Liver kinase B1 (LKB1) plays pivotal roles in cellular processes such as apoptosis, metabolism, and cell cycle regulation. (omicsdi.org)
  • High-fat, low-carbohydrate (CHO) diets increase whole-body rates of fat oxidation and down-regulate CHO metabolism. (edu.au)
  • The manipulation of blood flow in conjunction with skeletal muscle contraction has helped us with the physiological understanding of muscle fatigue, blood pressure reflexes, and metabolism in humans. (pensummed.pro)
  • Discovery of active human brown adipose tissue (BAT) has renewed interest in the possibility that it may regulate metabolism and EE in humans [ 3 - 5 ]. (biomedcentral.com)
  • Understanding the mechanisms controling BAT differentiation may identify potential therapeutic targets to enhance BAT-controlled metabolism and to increase EE. (biomedcentral.com)
  • Myokines are peptides or proteins released from skeletal muscle and are involved in multiple physiological processes, including metabolism and hypertrophy in the autocrine and paracrine manner [ 6 ]. (biomedcentral.com)
  • Although it is expressed in both the epaxial (to become muscles of the back) and hypaxial (body wall and limb muscles) portions of the myotome, it is regulated differently in these tissue lines, providing part of their alternative differentiation. (wikipedia.org)
  • The use of a muscle creatine kinase germline knockout of the gene encoding Ire1/Ern1 shifts the focus from traditional studies of tissue repair toward the regenerating myofibers. (elifesciences.org)
  • Skeletal muscle, the most abundant tissue of the body, has remarkable regenerative capacity mainly due to its resident muscle stem cells, also known as satellite cells. (elifesciences.org)
  • More proliferation was observed in muscle tissue of LBW-GLN than LBW-ALA piglets at 5 dpn, but there was no clear effect of supplementation on related gene expression. (nature.com)
  • Overall, Gln supplementation stimulated cell proliferation in muscle tissue and in vitro in myogenic cell culture, whereas muscle growth regulatory genes were barely altered. (nature.com)
  • Glutamine is regarded as the most abundant amino acid in the body, is mainly synthesized in skeletal muscle 10 , and muscle tissue is the most important site for Gln storage 11 . (nature.com)
  • Both miRNAs and siRNAs need to be delivered into the target tissue or cell in order to activate the desired therapeutic effect," Muthiah Manoharan, Ph.D., senior vp, drug discovery, Alnylam Pharmaceuticals, explained. (genengnews.com)
  • However, since there are many different non-muscle cell populations that reside within the tissue, these experiments cannot easily distinguish true myonuclei from those of neighboring mononuclear cells. (frontiersin.org)
  • Skeletal muscle is the largest tissue in the body. (frontiersin.org)
  • In this paper, we discuss the current available information about the role that different phenotypes of macrophages plays after injury and during the remodelling phase in different tissue types, with particular attention to the skeletal muscle. (hindawi.com)
  • Tissue-engineering and cell-based therapies, two prominent branches of Regenerative Medicine, are considered as the most suitable techniques to repair the tissue damage. (sciepub.com)
  • It's actually a special type of tissue in your body, where cells are fused together extra close - so close, that they behave a like a giant single cell. (robert-gorter.info)
  • A beta cell ATGL-lipolysis/adipose tissue axis controls energy homeostasis and body weight via insulin secretion in mice. (omicsdi.org)
  • The results support the concept that fuel excess can drive obesity and diabetes via hyperinsulinaemia, and that an islet beta cell ATGL-lipolysis/adipose tissue axis controls energy homeostasis and body weight via insulin secretion. (omicsdi.org)
  • Der muscle tissue and regulates sarcopenia [64]. (lckinhibitor.com)
  • Another study suggests that p53, by binding directly towards the myogenin promoter, can repress its transcription, impairing the maintenance of muscle tissue homeostasis [71]. (lckinhibitor.com)
  • In small mammals brown adipose tissue (BAT) plays a predominant role in regulating energy expenditure (EE) via adaptive thermogenesis. (biomedcentral.com)
  • To achieve this we firstly established a mouse BAT enriched-miRNA profile by comparing miRNAs expressed in mouse BAT, white adipose tissue and skeletal muscle. (biomedcentral.com)
  • Some steroids are more tissue selective, some will antagonize SHBG and/or Estrogen more than others, some will result in a dryer cosmetic look that differs from another, but when it comes to lean muscle growth, they all more or less do the same thing in a nitrogen retention context. (moreplatesmoredates.com)
  • The response of target cells is further regulated by their microenvironment, involving the extracellular matrix, which may be modified by MSC-produced matrix metalloproteinases (MMPs) and tissue inhibitor of MMPs. (biomedcentral.com)
  • MSCs can be defined by their ability to adhere to tissue culture plastic, their expression of several cell surface molecular epitopes-cluster of differentiation CD73, CD90, and CD105, and others-as well as their lack of several surface markers, including CD45 [ 6 ]. (biomedcentral.com)
  • MSCs can be isolated from a range of tissues, but the most commonly cited sources are bone marrow (BM), adipose tissue, muscle, bone, and perinatal tissues (e.g. (biomedcentral.com)
  • Cellular and molecular mechanisms regulating skeletal muscle development. (scielo.br)
  • The objective of this study was to elucidate the effects of early bFGF administration on the thyroarytenoid muscle after RLN transection and to investigate the underlying mechanisms. (biomedcentral.com)
  • This study will provide a theoretical reference for future studies on the molecular mechanisms of skeletal muscle development in goats. (koreascience.kr)
  • Possible MSY3 mechanisms of action have been studied in cell culture studies. (biomedcentral.com)
  • However, the mechanisms of cell proliferation induced by LPLI are poorly understood. (biomedcentral.com)
  • then we review the studies on the molecular mechanisms of LPLI-induced proliferation since January 1999, which will serve as a reference for the researchers in this field. (biomedcentral.com)
  • These cell lines are shared in collaboration with many international teams, either to study physiological or pathophysiological mechanisms, or to test innovative therapeutic approaches. (institut-myologie.org)
  • My research focuses on investigating the mechanisms responsible for sarcopenia (the age-related loss of skeletal muscle mass and strength) and developing exercise interventions for the treatment/prevention of sarcopenia. (uncg.edu)
  • Isolation and culture of individual myofibers and their satellite cells from adult skeletal muscle. (scielo.br)
  • Günther S, Kim J, Kostin S, Lepper C, Fan CM, Braun T. Myf5-positive satellite cells contribute to Pax7-dependent long-term maintenance of adult muscle stem cells. (scielo.br)
  • Inducible depletion of satellite cells in adult, sedentary mice impairs muscle regenerative capacity without affecting sarcopenia. (scielo.br)
  • Myofiber-specific ablation of IRE1α dampens Notch signaling and canonical NF-κB pathway in skeletal muscle of adult mice. (elifesciences.org)
  • Results: It was highly expressed in adult goat latissimus dorsi and leg muscles, goat fetal skeletal muscle, and at the differentiation stage of muscle satellite cells. (koreascience.kr)
  • Morgan and Partridge note that skeletal muscle satellite cells "are normally quiescent in adult muscle, but act as a reserve population of cells, able to proliferate in response to injury and give rise to regenerated muscle and to more satellite cells. (acupunctureofiowa.com)
  • We observed that, as skeletal muscle progressed from perinatal to postnatal and adult developmental stages, MSY3 protein became gradually dephosphorylated and accumulated in the nucleus. (biomedcentral.com)
  • Furthermore, forced expression of Akt in adult skeletal muscle induced MSY3 phosphorylation and myogenin derepression. (biomedcentral.com)
  • Given the significance of myogenin regulation in adult muscle, the Akt/MSY3/myogenin regulatory circuit is a potential therapeutic target to counteract muscle degenerative disease. (biomedcentral.com)
  • In murine-to-murine transplantation experiments, CXCR4 expression marks a population of adult murine satellite cells with robust engraftment potential in mdx mice, and CXCR4-positive murine muscle-derived SP cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice. (biomedcentral.com)
  • Adult mesenchymal stem cells (MSCs) represent a subject of intense experimental and biomedical interest. (biomedcentral.com)
  • To determine their possible roles in tissues and satellite cells in vitro, their expression pattern was examined in tissues from 40-day-old chickens and in satellite cells from the breast muscles of 1-week-old and 2-week-old chickens using RT-PCR and immunocytochemistry analyses. (scielo.br)
  • Therefore, we complemented our investigations into the cellular development of the skeletal muscle of neonatal piglets with in vitro studies using a primary porcine myogenic cell culture model with Gln supplementation. (nature.com)
  • In vivo , the identification of macrophage phenotype is complicated since macrophages are obviously exposed to a microenvironment that is more complex respect to cell culture conditions, and they display characteristics that do not conform to the in vitro defined phenotypic categories. (hindawi.com)
  • Since only small numbers of cells are obtainable from the patient, it requires expansion of cells in vitro . (sciepub.com)
  • Nevertheless, these primary cells grow slowly in vitro and lose their general characteristics during prolonged culturing period. (sciepub.com)
  • Skeletal muscle and myogenic C2C12 cells were used to study the effects of MSY3 phosphorylation in vivo and in vitro on its sub-cellular localization and activity, by blocking the IGF1/PI3K/Akt pathway, by Akt depletion and over-expression, and by mutating potential MSY3 phosphorylation sites. (biomedcentral.com)
  • Then, we comprehensively assessed the functional and histological parameters of skeletal muscle injury in mice and the phenotype shifting in macrophages through molecular biological methods and immunofluorescence analysis both in vivo and in vitro . (ijbs.com)
  • Preliminary results indicate that it also promotes brown adipocyte differentiation in vitro. (met-vasc.com)
  • Feeding with amino acids can stimulate protein synthesis in all tissues, especially in skeletal muscle as reviewed by Davis et al. (nature.com)
  • First, the expression of miR-188-5p in goat muscle tissues at different developmental stages was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). (koreascience.kr)
  • The expression level of the SFRP1 was highly varied across numerous pig tissues and it was down-regulated during porcine skeletal muscle development. (biomedcentral.com)
  • The light must be absorbed by the endogenous chromophores of cells or tissues for actions. (biomedcentral.com)
  • Exercise can produce Irisin and Semaphorin-3A by stimulating muscle and nerve cell, which have positive effects on other tissues, including the lungs. (jeoct.com)
  • In clinical practice, these involve the life-long immunosuppression of the organ transplantation patients, the creation of the supporting environment for engineered tissues in the mended organ, the unsolved issues of cell survival and differentiation of the cell-based therapy, and the selection and development of vectors for gene therapy. (biomedcentral.com)
  • The expression of L1-encoded proteins was independent from L1 RNA levels and differentially regulated in time and tissues. (sciencegate.app)
  • These data demonstrated that miR-101a promotes SMSC differentiation directly through EZH2 , which provides a theoretical reference for further elucidating the mechanism of miR-101a in SMSC differentiation. (chinagene.cn)
  • Conclusion: These results suggest that miR-188-5p inhibits the proliferation and promotes the differentiation of goat muscle satellite cells by targeting CAMK2B. (koreascience.kr)
  • Low power laser irradiation (LPLI) promotes proliferation of multiple cells, which (especially red and near infrared light) is mainly through the activation of mitochondrial respiratory chain and the initiation of cellular signaling. (biomedcentral.com)
  • Satellite cells help to repair and regenerate muscle fibers. (acupunctureofiowa.com)
  • Until recently, scientists thought this meant that nuclei - the cell control centers that build and maintain muscle fibers - are also lost to sloth. (robert-gorter.info)
  • Two independent studies - one in rodents and the other in insects - have demonstrated that nuclei are not lost from atrophying muscle fibers, and even remain after muscle death has been initiated. (robert-gorter.info)
  • Diprotin A stimulation of CXCR4, however, significantly increased the number of canine dystrophin-positive muscle fibers and canine-derived satellite cells by enhancing donor cell proliferation. (biomedcentral.com)
  • These cells are located beneath the basal lamina of myofiber in a dormant state. (elifesciences.org)
  • In the RAD51 supplier skeletal muscle, YAP positively regulates basal skeletal muscle mass and protein synthesis. (lckinhibitor.com)
  • When skeletal muscle is injured due to physical or chemical insult, a pool of self-renewing muscle stem cells residing within the skeletal muscles, called satellite cells, can give rise to differentiated myofibers to repair injured muscle ( Charge & Rudinicki, 2004 Chargé SBP, Rudnicki MA. (scielo.br)
  • There is a general agreement that hypertrophy is accompanied by the addition of new nuclei from stem cells to help the muscles meet the enhanced synthetic demands of a larger cell. (frontiersin.org)
  • His laboratory has followed two primary interests: 1) the transcription factor networks that regulate fate determination in various cells that make blood, and 2) the cell surface proteins expressed by hematopoietic stem cells that and allow them to communicate with their microenvironment. (ubc.ca)
  • Muscle growth is accompanied by the addition of new nuclei from stem cells to help meet the enhanced synthetic demands of larger muscle cells," explains Schwartz. (robert-gorter.info)
  • During adolescence muscle growth is enhanced by hormones, nutrition and a robust pool of stem cells, making it an ideal period for individuals to "bank" myonuclei that could be drawn upon to remain active in old age. (robert-gorter.info)
  • Transplantation of myogenic stem cells possesses great potential for long-term repair of dystrophic muscle. (biomedcentral.com)
  • The use of mesenchymal stem cells (MSCs) as a regenerative therapy holds the promise of regrowing injured vasculature, helping DM patients live healthier and longer lives. (thno.org)
  • Tumor vascular cells are critical for the growth of tumor stem cells, which reside within the vascular niche. (nih.gov)
  • One challenge with characterizing tumor stem cells has been finding appropriate conditions for in vivo growth. (nih.gov)
  • We therefore propose (3) to isolate ovarian tumor stem cells and grow them in vivo using the ESC ovarian cancer model. (nih.gov)
  • If successful, this will create a murine tumor model that nearly completely reproduces the human tumor microenvironment with human tumor stroma, vessels and tumor stem cells. (nih.gov)
  • Enforced expression of miR-101 inhibits prostate cancer cell growth by modulating the COX-2 pathway in vivo . (chinagene.cn)
  • Liver kinase B1 inhibits smooth muscle calcification via high mobility group box 1. (omicsdi.org)
  • Yamashita D, Machigashira M, Miyamoto M, Takeuchi H, Noguchi K, Izumi Y, Ban S. "Effect of surface roughness on initial responses of osteoblast-like cells on two types of zirconia. (sciepub.com)
  • Khadra M, Lyngstadaas SP, Haanaes HR, Mustafa K. "Effect of laser therapy on attachment, proliferation and differentiation of human osteoblast-like cells cultured on titanium implant material. (sciepub.com)
  • Significant loss of muscle mass may occur in cachexia and sarcopenia, which are major causes of mortality and disability. (mdpi.com)
  • As well, many older individuals suffer from sarcopenia, a protracted muscle wasting disorder that typically begins after the age of 50 and involves a loss of about 1% of muscle mass per year ( Woo, 2017 ). (frontiersin.org)
  • These and other data argue against the current interpretation of the myonuclear domain hypothesis and suggest that once a nucleus has been acquired by a muscle fiber it persists. (frontiersin.org)
  • These results support the hypothesis that MSY3 phosphorylation by Akt interferes with MSY3 repression of myogenin circuit activity during muscle development. (biomedcentral.com)
  • Differentiation begins (regulated by other genes) after this initial proliferation. (wikipedia.org)
  • Expression of muscle growth related genes was quantified with qPCR. (nature.com)
  • Meta-analysis of genome-wide association studies for cattle stature identifies common genes that regulate body size in mammals. (au.dk)
  • The SFRPs inhibit Wnt receptor binding to down-regulate pathway signaling during development [ 8 ]. (biomedcentral.com)
  • Hepatocyte growth factor receptor (c-Met), a member of tyrosine protein kinase receptors (TPKR), is phosphorylated during LPLI-induced proliferation, but tumor necrosis factor alpha (TNF-alpha) receptor has not been affected. (biomedcentral.com)
  • Still, the existence of these separate "MyoD-dependent" and "Myf5-dependent" subpopulations has been disputed, with some claiming that these MRFs are indeed coexpressed in muscle progenitor cells. (wikipedia.org)
  • Bober E, Franz T, Arnold HH, Gruss P, Tremblay P. Pax-3 is required for the development of limb muscles:a possible role for the migration of dermomyotomal muscle progenitor cells. (scielo.br)
  • Findings also indicated that acupuncture increased the number of progenitor and satellite cells. (acupunctureofiowa.com)
  • The changes of differentiation marker gene expression were detected by qPCR method. (koreascience.kr)
  • He performed some of the first studies to identify transcription factors that regulate the gene expression and differentiation of eosinophils, which are known to play a major role in allergic and asthmatic responses. (ubc.ca)
  • In muscle aging, the altered shuttle of nNOS towards the nucleoskeleton [76] determines a fail in p53 S-nitrosylation, which benefits in MuRF-1 gene expression upregulation [77]. (lckinhibitor.com)
  • Gene expression profiling of porcine skeletal muscle in the early recovery phase following acute physical activity. (au.dk)
  • It functions during that time to commit myogenic precursor cells to become skeletal muscle. (wikipedia.org)
  • The stimulation can be very carefully regulated for your comfort. (acupunctureofiowa.com)
  • While acknowledging progress in the other areas mentioned, this work will focus on the current debates concerning sourcing, MSC alterations of angiogenesis, cell differentiation/stimulation, and strategies to improve MSC differentiation. (biomedcentral.com)
  • There he worked with Dr. Max D. Cooper (Howard Hughes Medical Institute, National Academy of Sciences) and his research focused on cell surface proteins expressed by preB cells that regulate B cell maturation and homing. (ubc.ca)
  • In addition, he identified a number of novel hematopoietic stem cell surface proteins and began analyzing their function. (ubc.ca)
  • In this regard, his lab has identified a novel family of hematopoietic cell surface proteins, called the CD34 family, and shown that these are essential for a number of developmentally important processes. (ubc.ca)
  • Recently, the signaling proteins involved in LPLI-induced proliferation merit special attention, some of which are regulated by mitochondrial signaling. (biomedcentral.com)
  • LPLI-induced cell cycle progression can be regulated by the activation or elevated expressions of cell cycle-specific proteins. (biomedcentral.com)
  • Recently, a large number of signaling proteins reported play an important key role in the process of LPLI-induced cell proliferation, probably due to the fact that the molecular events they are involved in are the basic response of the cells to extracellular stimuli. (biomedcentral.com)
  • We hypothesize that the ESC ovarian tumor model, with human vascular cells, will provide an ideal microenvironment to support human stem cell growth. (nih.gov)
  • The purpose of this brief review is to discuss whether or not ambulatory exercise elicits leg muscle hypertrophy in older adults. (researchgate.net)
  • In addition, slow-walk training with a combination of leg muscle blood flow restriction elicits muscle hypertrophy only in the blood flow restricted leg muscles. (researchgate.net)
  • Competitive marathon running and regular high intensity distance running in young and middle-aged adults may not produce leg muscle hypertrophy due to insufficient recovery from the damaging running bout, although there have been no studies that have investigated the effects of running on leg muscle morphology in older subjects. (researchgate.net)
  • It is clear that skeletal muscle hypertrophy can occur independently of exercise mode and load. (researchgate.net)
  • It has been generally accepted that muscle hypertrophy requires high-intensity training utilizing loads of at least 70% of 1-repetition max or lower loads until failure. (pensummed.pro)
  • However, there is mounting evidence that now supports low-load resistance training (20-40%RM) combined with blood flow restriction can similarly induce muscle hypertrophy and strength gains. (pensummed.pro)
  • What we thought we knew and understood about muscle cell physiology and hypertrophy has been turned upside down through blood flow restriction research. (pensummed.pro)
  • This study aimed to determine the efficacy of functional training with and without blood flow restriction (BFR) on muscle hypertrophy indices and strength in older men. (biomedcentral.com)
  • The findings showed that FT was effective in increasing circulating biomarkers involved in hypertrophy in older adults while adding BFR to FT had a slight increase in these biomarkers but had a tremendous increase in muscle strength. (biomedcentral.com)
  • microRNAs in skeletal muscle differentiation and disease. (chinagene.cn)
  • This study elucidated effects of glutamine (Gln) supplementation on the cellular muscle development of LBW and normal birth weight (NBW) piglets. (nature.com)
  • We observed a temporarily increased intramuscular availability of free Gln that could have influenced cellular processes in skeletal muscle. (nature.com)
  • Musgrove E, Seaman M, Hedley D. "Relationship between cytoplasmic pH and proliferation during exponential growth and cellular quiescence. (sciepub.com)
  • Furthermore, Myf5 is a master regulator of muscle development, possessing the ability to induce a muscle phenotype upon its forced expression in fibroblastic cells. (wikipedia.org)
  • This expression is eventually repressed to prevent extraneous muscle formation. (wikipedia.org)
  • Upon increasing culture times in chicken´s primary skeletal muscle satellite cells, SULF1 and SULF2 expression in 1-week-old chickens was significantly higher than in 2-week-old chickens, suggesting that sulfatases play a key role in satellite cell development. (scielo.br)
  • In addition, we showed that during SMSC differentiation, the downregulated levels of EZH2 mRNA and protein were accompanied by increasing miR-101a expression via qRT-PCR and Western blot. (chinagene.cn)
  • Anderson C, Catoe H, Werner R. MIR-206 regulates connexin43 expression during skeletal muscle development. (chinagene.cn)
  • Crist CG, Montarras D, Pallafacchina G, Rocancourt D, Cumano A, Conway SJ, Buckingham M. Muscle stem cell behavior is modified by microRNA-27 regulation of Pax3 expression. (chinagene.cn)
  • Knocking down Akt expression increased the amount of dephosphorylated MSY3 and reduced myogenin expression and muscle differentiation. (biomedcentral.com)
  • While Akt over-expression rescued myogenin expression in MSY3 overexpressing myogenic cells, ablation of the Akt substrate, (Ser126 located in the MSY3 cold shock domain) promoted MSY3 accumulation in the nucleus and abolished this rescue. (biomedcentral.com)
  • This study highlights a previously undescribed Akt-mediated signaling pathway involved in the repression of myogenin expression in myogenic cells and in mature muscle. (biomedcentral.com)
  • Intramuscular injection of donor muscle-derived cells into chimeric cxmd recipients restored dystrophin expression for at least 24 weeks in the absence of post-transplant immunosuppression, indicating that cell transplantation may be a viable therapeutic option for muscular dystrophy. (biomedcentral.com)
  • We used the xenotransplant model to show that CXCR4 expression on canine donor muscle cells is also important for cell engraftment, but FACS sorting for CXCR4-positive cells decreased their engraftment efficiency. (biomedcentral.com)
  • We serially evaluate several facets of the recovery process, including α V β 3 -integrin expression (a marker of angiogenesis), blood perfusion, and muscle function. (thno.org)
  • We hypothesize that ovarian cancer cells will induce human ovarian TVM expression. (nih.gov)
  • It's been suggested that miRNAs regulate up to 50% of all mRNAs in the human genome. (genengnews.com)
  • Moreover, miRNAs play a modulatory role in the development and growth of skeletal muscles [ 22 ]. (biomedcentral.com)
  • Three miRNAs, miRNA-1, −133 and −206, are specifically expressed in muscle and are considered to be myomiRs [ 23 , 24 ]. (biomedcentral.com)
  • Heparan sulfate proteoglycans (HSPGs) are present on the cell surface and in the extracellular matrix in all metazoans. (scielo.br)
  • When activated by injury, satellite cells reenter the cell cycle and proliferate in response to extracellular growth factors ( Relaix & Zammit, 2012 Relaix F, Zammit PS. (scielo.br)
  • The proliferation and differentiation of satellite cells are regulated by a number of extracellular signals ( Wang & Rudnicki, 2012 Wang YX, Rudnicki MA. (scielo.br)
  • Rosiglitazone attenuates the metalloprotease/anti-metalloprotease imbalance in emphysema induced by cigarette smoke: involvement of extracellular signal-regulated kinase and NFκB signaling. (jeoct.com)
  • These MSC influences can range from relatively rare activities that require cell contact, such as mitochondrial transfer and cell fusion, to relatively common paracrine MSC actions through extracellular microvesicles or secreted factors. (biomedcentral.com)
  • Ex vivo cultures of myofiber explants demonstrate that ablation of IRE1α reduces the proliferative capacity of myofiber-associated satellite cells. (elifesciences.org)
  • The miRNA-mRNA profiles and bioinformatics study suggested that the SFRP1 gene was potentially regulated by miRNA-1/206 during porcine skeletal muscle development. (biomedcentral.com)
  • Dual luciferase assay and Western-blot results demonstrated that SFRP1 was a target of miR-1/206 in porcine iliac endothelial cells. (biomedcentral.com)
  • MicroRNA identity and abundance in porcine skeletal muscles determined by deep sequencing. (au.dk)
  • Some evidence shows that Myf5 and MyoD are responsible for the development of separate muscle lineages, and are not expressed concurrently in the same cell. (wikipedia.org)
  • Our team has demonstrated some important differences between man and mouse, in the regulation of the proliferation of myogenic precursors. (institut-myologie.org)
  • Therefore, in collaboration with W. Wright, we have developed an approach for the immortalisation of human muscle precursors that allows them to retain their ability to differentiate: this has enabled the generation of immortal cell lines derived from patient cells. (institut-myologie.org)
  • He has delineated the function of these molecules in diverse set of biological processes including: 1) gut and kidney formation, 2) vascular permeability, 3) mucosal inflammatory disease, 4) stem cell homing and migration, and 5) epithelial tumor progression. (ubc.ca)
  • Nature Reviews Molecular Cell Biology 2012;13(2):127-33. (scielo.br)
  • Through gene knockout studies he has shown that these molecules act as a type of molecular "Teflon" to make cells more mobile and invasive and also facilitate chemotaxis. (ubc.ca)
  • Molecular motors lie at the heart of biological processes from DNA replication to cell migration. (nih.gov)
  • The delayed growth of LBW piglets is mainly due to a slower development of skeletal muscle as the myogenic activity is usually impaired in these animals during the fetal and early postnatal period 3 , 4 . (nature.com)
  • We have previously shown that MSY3/Csda binds a highly conserved cis-acting element located upstream of the myogenin promoter (myogHCE) and regulates the postnatal down-regulation of myogenin [ 16 ]. (biomedcentral.com)
  • The Y-box protein MSY3/Csda represses myogenin transcription in skeletal muscle by binding a highly conserved cis-acting DNA element located just upstream of the myogenin minimal promoter (myogHCE). (biomedcentral.com)
  • BFRT exercise alters acute physiological stressors such as local muscle oxygen availability and vascular shear stress which may lead to adaptations that are not easily attained with conventional training. (pensummed.pro)
  • Vascular endothelial growth factor (VEGF) is important for the maintenance of the skeletal muscle capillaries. (uncg.edu)
  • Recently, we observed that muscle capillarization, vascular endothelial growth factor (VEGF) protein, and the VEGF mRNA response to acute exercise were lower in aged compared with young women. (uncg.edu)
  • Finally, our preliminary data suggest that the ESC-ovarian tumor model has human tumor vascular cells. (nih.gov)
  • However, diprotin A, a positive modulator of CXCR4-SDF-1 binding, significantly enhanced engraftment and stimulated sustained proliferation of donor cells in vivo . (biomedcentral.com)
  • The comprehensive functional tests and histological analysis in vivo indirectly and directly proved the effectiveness of LIPUS for muscle recovery. (ijbs.com)
  • Depletion of macrophages in vivo eliminated the therapeutic effects of LIPUS, indicating that improvement in muscle function was the result of M2-shifted macrophage polarization. (ijbs.com)
  • MicroRNA-133 controls brown adipose determination in skeletal muscle satellite cells by targeting Prdm16. (scielo.br)
  • To directly assess the role of beta cell lipolysis in insulin secretion and whole-body energy homeostasis, inducible beta cell-specific adipose triglyceride lipase (ATGL)-deficient (B-Atgl-KO) mice were studied under normal diet (ND) and high-fat diet (HFD) conditions. (omicsdi.org)
  • From a research, medical, and business standpoint, mesenchymal stem cell (MSC)-based therapies are fascinating. (biomedcentral.com)
  • There his work focused on transcriptional control of hematopoietic stem cell maturation and cell fate. (ubc.ca)
  • Immortalized Canine Dystrophic Myoblast Cell Lines for Development of Peptide-Conjugated Splice-Switching Oligonucleotides. (institut-myologie.org)
  • The high redundancy of this system shows how crucial the development of skeletal muscle is to the viability of the fetus. (wikipedia.org)
  • Our results indicate that the SFRP1 gene is regulated by miR-1/206 and potentially affects skeletal muscle development. (biomedcentral.com)
  • We used a clinically acceptable regimen of hematopoietic stem cell transplantation to establish mixed donor/host blood cell chimerism and immune tolerance in a canine model of Duchenne muscular dystrophy ( cxmd ) [ 9 ]. (biomedcentral.com)
  • In living organisms, if the temperature exceeds the normal range (thermo-neutral zone), it disturbs the normal physiological functioning and induces cell injury. (frontiersin.org)
  • Cell proliferation is a very important physiological effect for low power laser irradiation (LPLI) used in clinical practice. (biomedcentral.com)
  • Increased oxidative stress within the inactive muscle derives from the imbalance amongst the muscle anti-oxidant defense, reduced by the enhance of protein catabolism, along with the physiological oxidant production [85]. (lckinhibitor.com)
  • The cross-sectional thyroarytenoid muscle area was significantly larger in the high-dose group than in the saline and low-dose groups on days 28 and 56. (biomedcentral.com)
  • Immunohistochemistry indicated that bFGF significantly increased the number of satellite cells in the thyroarytenoid muscle up to day 14 and that of neuromuscular junctions on days 28 and 56. (biomedcentral.com)
  • Compared with the control group, the cell death index of the electroacupuncture (EA) group was significantly improved, indicating that acupuncture reduced muscle cell death. (acupunctureofiowa.com)
  • They have proinflammatory functions: they produce effector molecules (reactive oxygen and nitrogen intermediates) and inflammatory cytokines (IL-1 β , TNF α , and IL-6) and participate as inducer and effector cells in polarized Th1 responses. (hindawi.com)
  • But modern cell-type-specific dyes and genetic markers have shown that the dying nuclei other researchers had detected were in fact inflammatory and other cells recruited to atrophic muscle. (robert-gorter.info)
  • We measured substrate utilization and skeletal muscle mitochondrial respiration to determine whether these adaptations are driven by high fat or low CHO availability. (edu.au)
  • After 5 d of HFAT, skeletal muscle mitochondrial respiration supported by octanoylcarnitine and pyruvate, as well as uncoupled respiration, was decreased at rest, and rates of whole-body fat oxidation were higher during exercise compared with HPRO. (edu.au)