Cells progression from g1 phase cell cycle g1 progression. Medical search. Frequent questions

In recent years, epithelial-mesenchymal transition (EMT) has been suggested to play a key role in the process of embryonic development, differentiation of tissues and organs, chronic inflammation and fibrosis, as well as cancer progression ( 14 ). (spandidos-publications.com)
Genetic abnormalities associated with MDS block differentiation of hematopoietic stem and progenitor cells. (medscape.com)
As activation of c-myc expression promotes cell proliferation and blocks differentiation, it may positively regulate cell growth. (wdv.com)
Notably, it promotes the osteogenic differentiation of bone mesenchymal stem cells (BMSCs) [ 15 - 17 ]. (hindawi.com)
All-trans retinoic acid (RA) is the most active metabolite of vitamin A. Several studies have described a pivotal role for RA signalling in different biological processes such as cell growth and differentiation, embryonic development and organogenesis. (mdpi.com)
Additionally, the in vitro effects of PTE on osteoclastic activities were investigated using the RAW 264.7 cell line as an osteoclast differentiation model. (researchgate.net)
Moreover, in the in vitro studies, osteoclast differentiation using the differentiation cell model was significantly inhibited by PTE without cytotoxic effects. (researchgate.net)
Neuronal differentiation and cell-cycle programs mediate response to BET-bromodomain inhibition in MYC-driven medulloblastoma. (cancerindex.org)
and cell differentiation ( SOX2 and TGFB3 ) as well as immunohistochemical assay for VEGFA, TP53, Bcl2, TGFB1, and Ki67 protein expression have been performed in 85 FFPE RCC tumor specimens. (hindawi.com)
An aberrant miRNA expression could contribute to cancer development and progression [ 6 , 7 ] and could affect their target genes that are involved in many biological processes, such as cell differentiation, proliferation, apoptosis, metabolism, and development [ 8 ]. (hindawi.com)
It is involved in p53 pathways and is implicated in cell death/survival signaling, the cell cycle, and differentiation, thereby playing a regulatory role in carcinogenesis [ 12 ]. (hindawi.com)
For the developer, cell differentiation is the key to cancer therapy. (cancer.gov)
Antineoplastons are the surveillance system that directs cancer cells into normal channels of differentiation . (cancer.gov)
The notion of controlling tumor growth through a naturally occurring biochemical mechanism in the body that directs cancer cells into normal channels of differentiation is one of the theoretical foundations of antineoplaston therapy. (cancer.gov)
The proposed components in the body that correct the differentiation problems of abnormal cells and send them into normal pathways have been given the name "antineoplastons" by the developer. (cancer.gov)
The hallmark of embryonic stem (ES) cells is their ability for self-renewal (capability of unlimited cell division without the loss of pluripotency) as well as for differentiation into all cell types of the adult organism. (uni-muenchen.de)
A high expression of Pes1, Bop1 and WDR12 was observed in ES cells, which strongly decreased during in vitro differentiation. (uni-muenchen.de)
The SRY-box 4 (SOX4) gene, which is located on chromosome 6p22.3, encodes a 47-kDa protein that is a member of the sex-determining region Y-related high-mobility group-box transcription factor family and has functions in embryonic development and cell differentiation ( 3 , 4 ). (spandidos-publications.com)
The differentiation is mostly driven by the intensity of PhI, that almost doubles during cellular progression. (elettra.eu)
Key findings: Among five flavonoids isolated from GFF, only apigenin potently inhibited the differentiation of 3T3-L1 cells. (kist.re.kr)
Apigenin-treated 3T3-L1 cells failed to undergo clonal expansion during the early phase of adipocyte differentiation. (kist.re.kr)

Wu X, Song M, Qiu P, Li F, Wang M, Zheng J, Wang Q, Xu F, Xiao H. A metabolite of nobiletin, 4'-demethylnobiletin and atorvastatin synergistically inhibits human colon cancer cell growth by inducing G0/G1 cell cycle arrest and apoptosis. (umassmed.edu)

Cell proliferation includes a series of events that is tightly regulated by several checkpoints and layers of control mechanisms. (frontiersin.org)
Many more investigations have been made on cells, sorted according to size, or artificially arrested at various cell cycle checkpoints. (frontiersin.org)
p53 and its transcriptional targets play an important role in both G1 and G2 checkpoints. (kegg.jp)
G1 Phase Cell Cycle Checkpoints" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
This graph shows the total number of publications written about "G1 Phase Cell Cycle Checkpoints" by people in this website by year, and whether "G1 Phase Cell Cycle Checkpoints" was a major or minor topic of these publications. (umassmed.edu)
Below are the most recent publications written about "G1 Phase Cell Cycle Checkpoints" by people in Profiles. (umassmed.edu)

Loss of MCU prevents mitochondrial fusion in G1-S phase and blocks cell cycle progression and proliferation. (ucsf.edu)
Rb blocks cell cycle progression in G1 by binding E2F transcription factors. (shu.edu)

The expression of p53 is directly linked to a blockage of cell cycle progression at G0/G1 phase and upregulates Bax, cytochrome studies have shown the ability of the bioactive compounds of flavokawain B and alpinetin to target UCK2 enzyme specifically, inducing cell cycle arrest and subsequently leading to cancer cell death, possibly through interfering the MDM2-p53 signalling pathway. (nanoker-society.org)

This is linked to the lack of a functional G1/S-phase checkpoint, which allows the cells to enter the S-phase almost directly after mitosis. (uni-muenchen.de)
Mitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps known as G1 and G2 phases. (kegg.jp)
The present study confirmed previous observations by others of a progression from G0 through G1 and S phase to G2/mitosis during the first 3 d in culture. (rupress.org)
Cell-cycle manipulation with vinblastine and 5-bromo-2-deoxyuridine (BUdR) revealed that previous passage through both S phase and mitosis was required for entry into this newly observed late phase. (rupress.org)
In yeast and Drosophila, Cdh1-dependent APC (Cdh1-APC) activity targets mitotic cyclins from the end of mitosis to the G 1 phase. (elsevierpure.com)
In yeast and Drosophila, Cdh1-dependent APC (Cdh1-APC) activity targets mitotic cyclins from the end of mitosis to the G1 phase. (elsevierpure.com)
Alternatively, in the quantitative model, the gradual rise of Cdk activity from G1 phase to mitosis leads to ordered substrate phosphorylation at sequential thresholds. (crick.ac.uk)

The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). (thermofisher.com)
The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. (cancerindex.org)
These kinases are expressed throughout the cell cycle, but are only activated upon complex formation with their corresponding cyclins. (shu.edu)
However, similarly to yeast and Drosophila, loss of Cdh1 induced unscheduled accumulation of mitotic cyclins in G1, resulting in abrogation of G1, arrest caused by treatment with rapamycin, an inducer of p27Kipl. (elsevierpure.com)
Here, we study the relative contributions of qualitative and quantitative Cdk control in Saccharomyces cerevisiae All S phase and mitotic cyclins can be replaced by a single mitotic cyclin, albeit at the cost of reduced fitness. (crick.ac.uk)
A single cyclin can also replace all G1 cyclins to support ordered cell cycle progression, fulfilling key predictions of the quantitative model. (crick.ac.uk)
As a biologically important example we have studied the complex formed by cyclins and cyclin-dependent kinases (CDKs), which play an essential role in the control of the eukaryotic cell cycle. (lu.se)

Serum release of serum-starved (G 0 ) 5L rat hepatoma cells triggers transient AhR activation and P4501A1 protein expression concomitant with the G 0 /G 1 -to-S phase transition. (aspetjournals.org)
Our transcriptome analysis revealed in bladder cancer (BCa) tissues a significant induction of lysosomal-associated multispanning membrane protein 5 (LAPTM5), a lysosomal membrane protein preferentially expressing in immune cells and hematopoietic cells. (spandidos-publications.com)
LAPMT5 is a lysosomal membrane protein preferentially expressed in immune cells ( 5 , 6 ) and hematopoietic cells ( 7 ), having a close interaction with the Nedd4 ( 8 ), a member of the E3 ubiquitin ligases family ( 8 ). (spandidos-publications.com)
Abrogation by c-myc of G1 phase arrest induced by RB protein but not by p53. (wdv.com)
Replacement of wild-type RB can suppress the tumorigenicity of some of these cells, suggesting that the RB protein (Rb) may negatively regulate cell growth. (wdv.com)
It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. (thermofisher.com)
The protein p53 is one of the most important tumor suppressor proteins: its activation can lead to cell cycle arrest, DNA repair, or apoptosis. (biomedcentral.com)
CyclinE is a cell cycle regulatory protein which regulates the G1-S phase transition during cell proliferation. (biomedcentral.com)
By performing cell biological and biochemical experiments using human cells, Moyer and Holland show that PLK4 interacts with a protein called STIL that is found in the central part of the centriole. (elifesciences.org)
The modification of STIL at a specific location by PLK4 was needed to link it to another protein in the outer wall of the centriole, and was also necessary for the cells to build new centrioles. (elifesciences.org)
ATR-Chk1-mediated protein degradation of Cdc25A protein phosphatase is also a mechanism conferring intra-S-phase checkpoint activation. (kegg.jp)
Tumour suppressor protein, p53, prevents cancer development by eliminating cells with mutagenic alterations or potential for neoplastic transformation or blocking their cell cycle permanently or by transient DNA repair [3C5]. (nanoker-society.org)
Extraction of total protein Total protein was extracted from lysed HT-29 cells in an appropriate volume of ProteoJET mammalian cell lysis reagent (Fermentas, Burlington, ON, Canada). (nanoker-society.org)
ST caused G0/G1 cell cycle arrest which was accompanied by a decrease in CDK4 and cyclin D1, and an increase in p21/Cip1and p27/Kip1 protein levels. (biomedcentral.com)
Focusing on cyclin D1 as a major cell cycle regulator, we present evidence that a crucial cell cycle function of cyclin D1 is to prevent the transition into quiescence and identify a unique cyclin D1 protein kinetics that are indicative of very short G1 phases. (cam.ac.uk)
Treatment of breast cancer cell lines with the combination of palbociclib and antiestrogens leads to decreased retinoblastoma protein (Rb) phosphorylation resulting in reduced E2F expression and signaling and increased growth arrest compared to treatment with each drug alone. (shu.edu)
In addition, apigenin inhibited the DNA-binding activity of C/EBPβ in differentiating 3T3-L1 cells by down-regulating the 35 kDa isoform of C/EBPβ (liver-enriched activating protein) and up-regulating the expression of two different sets of C/EBP inhibitors: C/EBP homologous protein and the phospho-liver-enriched inhibitory protein isoform of C/EBPβ. (kist.re.kr)
p27 is a protein that binds to and prevents the activation of different G1. (lu.se)

In particular, ubiquitin-mediated degradation is critically important at transition points where it provides directionality and irreversibility to the cell cycle, which is essential for maintaining genome integrity. (intechopen.com)
Detailed analysis of cell cycle predictive genes allowed us to define subpopulations with distinct gene expression profiles and to calculate a cell cycle index that illustrates the transition of cells between cell cycle phases. (frontiersin.org)
Here, we employed single-cell gene expression profiling to describe the dynamic transition between cell proliferative states in three different cell lines using a panel consisting of 93 marker genes. (frontiersin.org)
Regulatory signaling systems that control the progression of the CELL CYCLE through the G1 PHASE and allow transition to S PHASE when the cells are ready to undergo DNA REPLICATION. (umassmed.edu)
Therefore, an improved understanding of the functions and the underlying molecular mechanisms of these proteins, particularly in epithelial-mesenchymal transition, may provide novel insights into the breast tumorigenesis and progression, and enable the development of effective anti-cancer therapeutics. (spandidos-publications.com)
I will present an all-in-one cell cycle reporter that allows simultaneous analysis of cell cycle progression, including the transition into quiescence, and the dynamics of individual fate determinants. (cam.ac.uk)
Virtually all mitogenic signals to the cell are processed through CDK4/6 - it is truly the central molecule that governs pRb phosphorylation status up through the R-point transition. (shu.edu)

Bufalin induces G0/G1 phase arrest through inhibiting the levels of cyclin D, cyclin E, CDK2 and CDK4, and triggers apoptosis via mitochondrial signaling pathway in T24 human bladder cancer cells. (umassmed.edu)
This study is the first to show that VALD-3 induces apoptosis and cell cycle arrest in human breast cancer cells by suppressing Wnt/β-catenin signaling, indicating that it could be a potential drug for the treatment of breast cancer. (researchsquare.com)

Our simulations show that, if HMGB1 is overexpressed, then the oncoproteins CyclinD/E, which regulate cell proliferation, are overexpressed, while tumor suppressor proteins that regulate cell apoptosis (programmed cell death), such as p53, are repressed. (biomedcentral.com)
CDKs regulate the cell's progression through the phases of the cell cycle by modulating the activity of key substrates. (kegg.jp)
To understand the molecular mechanism of SOX4 in breast cancer development and progression, the present study aimed to identify miRNAs that regulate the expression of SOX4, which revealed miR-320 as a potential candidate. (spandidos-publications.com)

These findings indicate miR-34a along with its putative target genes could play a role in RCC tumorigenesis and progression. (hindawi.com)
In the present study, the function and underlying molecular mechanisms of miR‑320 in breast cancer tumorigenesis and progression were investigated. (spandidos-publications.com)
Collectively, these results indicated that miR‑320 acts as a tumor suppressor in breast cancer tumorigenesis and progression. (spandidos-publications.com)

Treating serum-released 5L cells with the alkyne metabolism-based P4501A1 inhibitor 1-(1-propynyl)pyrene results in prolonged AhR activation, enhanced p27 Kip1 expression, and G 1 phase arrest after serum release. (aspetjournals.org)
Treatment of mouse NIH3T3 cells with 5FU, a potent inhibitor of rRNA maturation, confirmed an activation of this checkpoint, leading to weak induction of the tumor suppressor p53, induction of the Cdk-inhibitor p21, an increase in active, hypo-phosphorylated Rb, and to accumulation of cells in the G1- and S-phase with an increase of cells in G1-phase. (uni-muenchen.de)
Concretely, E2 or BPA decreased the gene expression of p21, which is a potent cyclin-dependent kinase (Cdk) inhibitor and responsible for the cell cycle arrest at G1 phase, to proliferate the BG-1 cells. (endocrine-abstracts.org)
We studied whether cellular senescence induced by HDAC inhibitor sodium butyrate in E1a+cHa-Ras -transformed rat embryo fibroblasts (ERas) and A549 human Ki-Ras mutated lung adenocarcinoma cells would enhance the tumor suppressor effect of MEK/ERK inhibition. (aging-us.com)
In a Phase 3 Paloma-III study of Pfizer's CDK4/6 inhibitor ( Ibrance - palbociclib) and an estrogen receptor antagonist ( Faslodex - fulvestrant), patients receiving the combination had significantly prolonged progression-free survival than women with breast cancer who received Faslodex, alone. (shu.edu)
Thus, activation of Cdh1-APC plays a crucial role in both cdk inhibitor-dependent G1 arrest and DNA damage-induced G2 arrest. (elsevierpure.com)

Dysregulation of microRNAs (miRs) can contribute to cancer development and progression. (spandidos-publications.com)
SOX4 has been demonstrated to be upregulated in a number of types of cancer, and increased SOX4 activity was found to contribute to cancer development and progression ( 5 - 8 ). (spandidos-publications.com)

[ 2 ] Options for second-line therapy in patients with relapsed/refractory disease include chemotherapy-free regimens with biologic targeted agents such as covalent Bruton tyrosine kinase (BTK) inhibitors, lenalidomide,venetoclax, and chimeric antigen receptor (CAR) T-cell therapy. (medscape.com)
Centriole duplication occurs once per cell cycle and is regulated by Polo-like kinase 4 (PLK4). (elifesciences.org)
Abstract Uridine-cytidine kinase 2 is an enzyme that is overexpressed in abnormal cell growth and its implication is considered a hallmark of malignancy. (nanoker-society.org)
Metformin (MTF) has been reported to target NLK (Nemo-like kinase) to inhibit non-small lung cancer cells. (cancerindex.org)
Two models have been put forward for cyclin-dependent kinase (Cdk) control of the cell cycle. (crick.ac.uk)

Flow cytometry was used to analyze the mitotic cell cycle during the mitotic clonal expansion phase. (kist.re.kr)

HLA- em DRB1 /em genotypes, high disease activity, high disability scores, and high levels of acute phase proteins [2]. (exposed-skin-care.net)
The cell cycle is strictly regulated and controlled by a complex network of signaling pathways [ 1 ], comprised of hundreds of proteins. (biomedcentral.com)
If some important proteins are mutated or there are defects in the signaling mechanisms, normal cell growth regulation will break down, possibly leading to the occurrence of cancer in the future. (biomedcentral.com)
Moreover, a number of extracellular proteins can bind to their receptors and activate signaling pathways that promote the proliferation of cancer cells. (biomedcentral.com)
The HMGB1 signal transduction can influence the cell's fate by two important processes - apoptosis and cell proliferation - which are regulated respectively by the proteins p53 and CyclinE, acting in two different signaling pathways. (biomedcentral.com)
During this work, the function of the proteins Pes1, Bop1 and WDR12, which were shown previously to be involved in ribosome biogenesis of mature cell lines, was investigated in mouse ES cells. (uni-muenchen.de)
Localization of the proteins was predominantly nucleolar and the formation of a stable complex (PeBoW-complex), including all three proteins, was experimentally validated in mature mouse cells as well as in mouse ES cells. (uni-muenchen.de)
When cells are about to divide, they make a new set of centrioles by assembling a variety of proteins. (elifesciences.org)
The expression of pro-apoptotic proteins and anti-apoptotic proteins in MCF-7 and MDA-MB-231 cells was investigated by Western blotting. (researchsquare.com)
A previous study demonstrated that various proteins are dysregulated in primary tumors, and are associated with breast cancer development and progression ( 2 ). (spandidos-publications.com)
Although live imaging has provided extensive insights into this relationship at the single-cell level, the limited number of fluorescent markers that can be used in a single experiment has hindered efforts to link the dynamics of individual proteins responsible for decision making directly to cell cycle progression. (cam.ac.uk)
In addition, to allow manipulating the levels of endogenous proteins at will, we introduce a novel approach to render already existing GFP (trap) lines amenable to auxin-dependent degradation by simple ectopic expression of a degradation cassette using human cells and zebrafish as a proofs of principle. (cam.ac.uk)

During cell growth and proliferation, ubiquitin plays an outsized role in promoting progression through the cell cycle. (intechopen.com)

Increasing evidence indicates that senescent cells could be a promising new target for therapeutic intervention known as senotherapy, which includes depleting senescent cells, modulating SASP and restoration of senescence inhibitors. (frontiersin.org)
Cyclin-CDK inhibitors (CKIs), such as p16Ink4a, p15Ink4b, p27Kip1, and p21Cip1, are involved in the negative regulation of CDK activities, thus providing a pathway through which the cell cycle is negatively regulated. (kegg.jp)
Despite a large number of promising inhibitors for Ras/Raf/MEK/ERK pathway, recent works show that cancer cells often develop an autophagy-dependent resistance to inhibitors of Ras pathway [ 16 ]. (aging-us.com)
Due to the selective expression of UCK2 in malignancy cells, a selective inhibition of this important enzyme necessitates the discovery of its potential inhibitors for malignancy chemotherapy. (nanoker-society.org)
Here, we employed the used of to investigate the effectiveness of natural UCK2 inhibitors to cause HT-29 cell death. (nanoker-society.org)

Here, we used quantitative real-time PCR, profiling the expression of 93 genes in single-cells from three different cell lines. (frontiersin.org)
We found that the total transcript level per cell and the expression of most individual genes correlated with progression through the cell cycle, but not with cell size. (frontiersin.org)
In conclusion, we provide useful experimental approaches and bioinformatics to identify informative and predictive genes at the single-cell level, which opens up new means to describe and understand cell proliferation and subpopulation dynamics. (frontiersin.org)
Also, the alteration of p21 gene expression by E2, BPA, or genistein affected the expression of its downstream genes of cell cycle, cyclin D1 and Cdk-4. (endocrine-abstracts.org)
The expression of further E2F target genes, as cyclin A, facilitates progression through S phase. (shu.edu)

A mutation in this gene resulting in reduced cell proliferation, and impaired cell motility and polarity, and has been identified in patients with primary microcephaly. (cancerindex.org)

During EMT, cells will undergo transformation from epithelial phenotype to mesenchymal phenotype ( 14 ) and many characteristics of cells will change including loss of cell-cell adhesion and acquisition of aggressive and metastatic ability ( 15 ). (spandidos-publications.com)
Here, we evaluated the efficacy of ST on three different human cancer cell lines including skin epidermoid carcinoma A431 cells, lung epithelial carcinoma A549 cells and breast adenocarcinoma MDA-MB-231. (biomedcentral.com)
SOX4 has been revealed to induce EMT, a key process during organ development and the progression of epithelial tumors to metastatic cancers ( 9 ), via its activation of the Wnt and transforming growth factor β signaling pathways in cancer cells ( 10 , 11 ). (spandidos-publications.com)

The properties of HUCMSCs were validated by untargeted label-free quantitation of the cell and CM proteome, suggesting increased activity of regeneration, autophagy and anti-inflammation pathways and mitochondrial function. (nature.com)

Taken together, our results suggested that decreased LAPTM5 inhibited proliferation and viability, as well as induced G0/G1 cell cycle arrest possibly via deactivation of ERK1/2 and p38 in BCa cells. (spandidos-publications.com)
Main methods: Cell viability of 3T3-L1 preadipocytes was assessed by MTT assays, and lipid accumulation was measured by Oil Red O. Adipogenesis related factors were checked by Western blot analysis. (kist.re.kr)
Cdh1 was dispensable for viability and cell cycle progression. (elsevierpure.com)

Furthermore, we established a BCa cell model with downregulated LAPTM5, revealing a significantly delayed growth rate in the BCa cells with knockdown of LAPTM5. (spandidos-publications.com)
Moreover, cell cycle arrest at G0/G1 phase was triggered by decreased LAPTM5 as well, which could lead to delayed BCa cell growth. (spandidos-publications.com)
Such data are only indirectly related to quantitative changes in cells at different states of division and growth. (frontiersin.org)
Generally, cells have to closely coordinate growth and cell cycle progression during proliferation to prevent premature division. (uni-muenchen.de)
One important factor for cell growth is ribosome biogenesis. (uni-muenchen.de)
When the body needs to make more cells, for example during growth or repair, existing cells divide in two in order to replicate themselves. (elifesciences.org)
In this study, we examined the effect of a phytoestrogen, genistein, on the cell growth of BG-1 ovarian cancer cells expressing estrogen receptors (ERs) caused by E2 and BPA. (endocrine-abstracts.org)
In the cell proliferation test in vitro, E2 or BPA increased the growth of the BG-1 ovarian cancer cells expressing ERs. (endocrine-abstracts.org)
On the other hand, genistein upregulated the expression of p21 gene cultured in the presence of E2 or BPA, leading to the growth inhibition of the BG-1 cells. (endocrine-abstracts.org)
By xenografting human breast cancer cells into nude mice, we found that VALD-3 significantly suppressed tumor cell growth while showing low toxicity against major organs. (researchsquare.com)
Cell growth assay, cell cycle analysis, FACS, JC-1 staining, annexin V staining and immunoblotting were used to study the efficacy of ST on cancer cells. (biomedcentral.com)
ST (30-90 μM) treatments for 48 h and 72 h did not show any significant effect on cell growth and death in A431 cells. (biomedcentral.com)
Whereas similar ST treatments moderately inhibited the growth of A549 cells by up to 13% (p ≤ 0.05) in 48 h and 14% (p ≤ 0.05-0.0001) in 72 h. (biomedcentral.com)
In MDA-MB-231 cells, ST caused a significant dose-dependent cell growth inhibition by 31- 63% (p ≤ 0.0001) in 48 h and 40-50% (p ≤ 0.0001) in 72 h. (biomedcentral.com)
The knowledge of cell cycle phase distribution is of paramount importance for understanding cellular behaviour under normal and stressed growth conditions. (elettra.eu)
Estrogen is a mitogenic growth factor - disrupting the production or effective signaling of estrogen deprives cells of signals to divide. (shu.edu)

Our results suggest that budding yeast has become dependent on G1 cyclin specificity to couple cell cycle progression to essential morphogenetic events. (crick.ac.uk)

Centrosomes nucleate the formation of the microtubule cytoskeleton in interphase cells and form the poles of the mitotic spindle during cell division. (elifesciences.org)

Erythropoietin is a glycoprotein hormone that stimulates red blood cell (RBC) production in bone marrow via binding to the cell-surface receptor on hematopoietic progenitor cells, and it has been widely used for treating anemia [ 13 ]. (hindawi.com)

The study exhibited that this expression of UCK2 mRNA were substantially reduced in treated HT-29 cells. (nanoker-society.org)

In addition, downregulation in expression of 18S ribosomal RNA was also observed in all treated HT-29 cells. (nanoker-society.org)

The aryl hydrocarbon receptor (AhR) transcription factor is increasingly recognized as functioning in cell cycle control. (aspetjournals.org)
The data are consistent with a cell cycle role for P4501A1 because they show that P4501A1 negatively regulates the duration of AhR action through the metabolic removal of the receptor agonist, thereby preventing AhR-mediated G 1 phase arrest. (aspetjournals.org)
Moreover, some studies demonstrated that LAPTM5 was highly expressed in malignant B lymphomas and involved in B cell malignancies ( 10 ), involving in negative regulation of cell surface T and B cell receptor by promoting lysosome degradation ( 6 ). (spandidos-publications.com)
In vitro, palbociclib reduced cellular proliferation of estrogen receptor (ER)-positive breast cancer cell lines by blocking progression of the cell from G1 into S phase of the cell cycle. (shu.edu)

2 ] The living cell is an autonomous cybernetic system connected to, and receiving, information from its environment through an energy pathway and an information pathway. (cancer.gov)
Hence, ribosomal stress does not lead to checkpoint activation via the p53-p21-Rb pathway in ES cells. (uni-muenchen.de)
The successful strategy based on the activation of cell death in Ras-expressing cells is associated with the suppression of kinases involved in Ras pathway. (aging-us.com)
Senescent ERas cells do not develop cytoprotective autophagy upon inhibition of MEK/ERK pathway due to spatial dissociation of lysosomes and autophagosomes in the senescent cells. (aging-us.com)
Our data show that suppression of MEK/ERK pathway in senescent cells provides a new strategy for elimination of Ras-expressing cells. (aging-us.com)
VALD-3 also regulated the Wnt/β-catenin signaling pathway in breast cancer cells, inhibiting the activation of downstream molecules. (researchsquare.com)

Piperine, a natural product from black pepper, has been demonstrated to suppress tumor cell proliferation by enhancing reactive oxygen species (ROS), subsequently leading to cell death. (bvsalud.org)

Eukaryotic cells respond to DNA damage by activating signaling pathways that promote cell cycle arrest and DNA repair. (kegg.jp)

Inactivating mutations of the retinoblastoma gene (RB) are found in a wide variety of tumour cells. (wdv.com)
Patients with ACVRL1 mutations who do develop PAH 7 are particularly young, have often rapid disease progression and have a worse prognosis than patients with BMPR2 mutations 10 . (nature.com)
PMID:18786442 mutations and the risk of esophageal squamous cell carcinoma. (who.int)

Mantle cell lymphoma (MCL) is recognized in the Revised European-American Lymphoma and World Health Organization classifications as a distinct clinicopathologic entity. (medscape.com)
Pope PA, Pryciak PM. Functional overlap among distinct G1/S inhibitory pathways allows robust G1 arrest by yeast mating pheromones. (umassmed.edu)
It therefore appears that, in this system, stimulated cells remain in a distinct cell-cycle phase for a number of hours before their return to the resting state. (rupress.org)
In the qualitative model, cell cycle events are ordered by distinct substrate specificities of successive cyclin waves. (crick.ac.uk)

This can provide Ras-expressing tumor cells additional selective advantages under stress or damage. (aging-us.com)
Many of the current efforts regarding anti-cancer drug development are focused on directing tumor cells to undergo apoptosis. (mdpi.com)
Possible mechanisms for overcoming the resistance of HPV-infected tumor cells to anticancer drugs will be discussed. (mdpi.com)

These remedies at best only slow, but not completely halt, CKD progression, and they are often ineffective and possess adverse side effects. (frontiersin.org)
DNA DAMAGE, or the deficiencies in specific cellular components or nutrients may cause the cells to halt before progressing through G1 phase. (umassmed.edu)

Co-injection of c-myc, but not EJ-ras, c-fos or c-jun, inhibits the ability of Rb to arrest the cell cycle. (wdv.com)
Further investigating the physiological activity of B5 in HeLa cells, we found that B5 selectively inhibits the activity of TrxR by binding to Sec residues on TrxR. (bvsalud.org)

Notably, the G1/S boundary represents a major barrier to cell proliferation and is universally dysfunctional in cancer cells, allowing for the unbridled proliferation observed in malignancy. (intechopen.com)
Numerous E3 ubiquitin ligases, which facilitate the ubiquitination of specific substrates, have been shown to control G1/S. In this chapter, we will discuss components in the ubiquitin proteasome system that are implicated in G1/S control, how these enzymes are interconnected, gaps in our current knowledge, and the potential role of these pathways in the cancer cycle and disease proliferation. (intechopen.com)
Furthermore, previous studies suggested that knockdown of LAPTM4B , another important subtype of the LAPTM family inhibited proliferation of hepatocellular carcinoma ( 11 ), prostate ( 12 ) and breast cancer cells ( 13 ). (spandidos-publications.com)
At the molecular level, abnormal cells that are potential cancer cells need to be "switched" to normal mode. (cancer.gov)
Which signaling pathways are fundamental for describing HMGB1 signal transduction, and what mechanisms are responsible to explain recent results linking overexpression of HMGB1 with decrease of apoptosis (and increased cancer cell survival)? (biomedcentral.com)
This editorial highlights the relevance of interfering in cancer cell progression through the pharmacological manipulation on the cell metabolism of cyclic nucleotides such as cAMP, and on the intracellular Ca2+ signaling, which may avail the reduction of toxic effects promoted by chemotherapy, radiotherapy and immunotherapy, thus decreasing the incidence of interruption in antitumoral treatment. (researchgate.net)
Their proliferation activity was reversed by the treatment of ICI 182,780, a well-known antagonist of ERs, which demonstrates that the cell proliferation by E2 or BPA is mediated by ERs and BPA certainly acts as a xenoestrogen in the BG-1 ovarian cancer cells. (endocrine-abstracts.org)
Cancer cells exploit a variety of protective mechanisms that allow them to acquire selective advantage and proliferate under unfavorable conditions. (aging-us.com)
Macroautophagy (hereafter referred to as autophagy) plays an important role in cancer cells survival upon various conditions of intra- and extracellular stress. (aging-us.com)
One of the mechanisms of high basal autophagy activity in cancer Ras-expressing cells might be associated with high PP2A phosphatase activity targeting directly ULK1-Ser757 that could explain apparent contradiction: maintenance of high mTORC1 functions and high autophagic activity simultaneously [ 22 ]. (aging-us.com)
In this study, we investigated the cytotoxic and apoptotic effects of VALD-3, a Schiff base ligand synthesized from o-vanillin derivatives, on human breast cancer cells and the possible underlying mechanisms. (researchsquare.com)
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-test was used to observe the proliferation of human breast cancer MCF-7 and MDA-MB-231 cells induced on VALD-3. (researchsquare.com)
Flow cytometry analysis showed that VALD-3 triggered cell cycle arrest and induced apoptosis of breast cancer cells. (researchsquare.com)
While exploring the molecular changes associated with strong ST efficacy in breast cancer cells, we observed that ST induced cell cycle arrest as well as cell death. (biomedcentral.com)
This study provides valuable insight into the chemopreventive efficacy and associated molecular alterations of ST in breast cancer cells whereas it had only moderate efficacy on lung cancer cells and did not show any considerable effect on skin cancer cells. (biomedcentral.com)
In addition, knockdown of miR‑320 in breast cancer cell lines promoted cell proliferation and invasion in vitro, whereas miR‑320 overexpression had the opposite effect. (spandidos-publications.com)
Previously, it has been demonstrated that certain transcriptional targets of SOX4 are associated with cancer development and progression and the processing of microRNAs (miRNAs/miRs) ( 12 ). (spandidos-publications.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Ibrance has now shown activity in combination with anti-estrogen therapy in patients with metastatic breast cancer both in the front-line and after progression following endocrine therapy. (shu.edu)
In vitro treatment of ER-positive breast cancer cell lines with the combination of palbociclib and antiestrogens leads to increased cell senescence, which was sustained for up to 6 days following drug removal. (shu.edu)
Anti-estrogen therapy involves blocking the production, binding, or signaling of estrogen in cancer cells. (shu.edu)
S1-Puro is selectively activated by TrxR and shows the TrxR-dependent cytotoxicity to cancer cells, while S2-Puro is readily activated by thiols. (bvsalud.org)
Three steps are described in the development of cancer namely initiation, promotion and progression. (bvsalud.org)

The functional regulation of cells is a complex and dynamic process. (nature.com)

Lymphocyte cell-cycle analysis by flow cytometry. (rupress.org)
we report on the use of FTIRM as technique alternative to flow cytometry using propidium iodide staining for studying cell cycle distribution in live cells. (elettra.eu)

Its roles include regulation of proliferation, promotion of immune system and activation of apoptosis in B cells. (intechopen.com)

The molecular processes behind cell cycle progression have been dissected by numerous morphological studies on live or fixed single cells using a plethora of techniques to visualize components and processes during cell division. (frontiersin.org)
Moreover, alkaline phosphatase activity was equally detectable after depletion of Pes1 or Bop1 and no morphological changes within the ES cell colonies were observed. (uni-muenchen.de)
TIF) pone.0170233.s001.tif (215K) GUID:?4A59CDD6-F306-4806-8FCD-576435A1A3FA S2 Fig: Morphological examination of HT-29 cells treated with crude hexane extract at IC25: 10.52, IC50: 21.05, and IC75:42.1 g/mL. (nanoker-society.org)

Mantle cell lymphoma (MCL) is a lymphoproliferative disorder derived from a subset of naive pregerminal center cells localized in primary follicles or in the mantle region of secondary follicles. (medscape.com)
Mantle cell lymphoma (MCL) with a nodular and diffuse pattern associated with hyalinized blood vessels. (basicmedicalkey.com)
Cyclin-D1 immunostain shows nuclear expression in the cells of mantle cell lymphoma. (basicmedicalkey.com)

Specifically, the boundary between G1 and S-phase is tightly regulated by the ubiquitin proteasome system. (intechopen.com)

In contrast, sustained AhR activation in response to TCDD treatment increases p27 Kip1 expression in addition to P4501A1, resulting in G 1 phase cell cycle arrest. (aspetjournals.org)
In mature cells, disruptions in ribosome biogenesis are directly linked to the cell cycle machinery by a p53-dependent activation of the G1/S-phase checkpoint, leading to an arrest of cells in G1-phase. (uni-muenchen.de)
Precise activation and inactivation of CDKs at specific points in the cell cycle are required for orderly cell division. (kegg.jp)
During early G1 phase, mitogenic signals trigger activation of the CDK4/6-cyclin D complex, which partially deactivates Rb by phosphorylation. (shu.edu)

For pediatric patients with refractory cytopenia, certain cytogenetic abnormalities, or malignant transformation, hematopoietic stem cell transplantation (HSCT) from a matched related or unrelated donor early in the course of the disease is the treatment of choice. (medscape.com)
According to recent data, Ras-transformed cells require autophagy to survive and maintain malignant phenotype [ 5 - 10 ]. (aging-us.com)
oxidation modification of the genetic material may also participate in the progression of benign to malignant neoplasms. (bvsalud.org)

In contrast, no significant alteration of apoptosis in the BCa cells with downregulated LAPTM5 was noticed. (spandidos-publications.com)
In contrast, ES cells showed strong induction of p53, but no induction of its target gene p21. (uni-muenchen.de)
In contrast to its normal counterparts, the initiated cell can escape from cellular control mechanisms when responding to external or intercellular signals 5 . (bvsalud.org)

Analysis of the changes of migration and invasion, showed significant reduced LAPTM5 suppressed cell metastasis. (spandidos-publications.com)
A new investigation proposing piRNA as a prognostic and diagnostic biomarker in the treatment of CRC Therefore, this review focuses on the role of piRNAs in the initiation, progression, and metastasis of CRC and its molecular mechanisms to understand its function and provide a better therapeutic strategy. (jcimcr.org)

The signaling mechanisms of IL-21 in different cell types have been identified. (intechopen.com)
However, we know less about the biological effects of IL-21 and its signaling mechanisms in leukemia cells and monocytes. (intechopen.com)
Overall, the results suggest that ES cells use different mechanisms as mature cells to coordinate their proliferation rate with ribosome biogenesis. (uni-muenchen.de)
To avoid elimination by this mechanism, human papillomaviruses (HPV) have developed several mechanisms that enable the cells they infect to elude both extrinsic and intrinsic apoptosis. (mdpi.com)
In this manuscript, we review the current literature regarding how HPV-infected cells avoid apoptosis and the molecular mechanisms involved in these events. (mdpi.com)
Two mechanisms are involved in the generation of mature piRNAs: a primary synthesis mechanism that is carried out in both somatic and germ cells and a 'ping pong' amplification mechanism that is only formed in the germline [9,20]. (jcimcr.org)

This event explains the presence of multiple derangements observed in the bone marrow that involve several cell lineages. (medscape.com)
As the affected cell lines continue to divide and to provide the marrow with dysplastic cells, bone marrow dysfunction becomes apparent. (medscape.com)
The effects of erythropoietin (EPO) on the behaviors of bone marrow mesenchymal stem cells (BMSCs) subjected to mechanical stretch remain unclear. (hindawi.com)
Myelodysplastic syndrome (MDS) in childhood encompasses a diverse group of bone marrow disorders that share a common clonal defect of stem cells and that result in ineffective hematopoiesis with dysplastic changes in the marrow. (medscape.com)

Low AKAP12 expression in a larger cohort of patients (75) characterized tumor invasiveness, recurrence, and progression, indicating its potential as a prognostic biomarker. (nature.com)

Aberration occurs in a stem cell that can give rise to multiple lineages. (medscape.com)

Several recent reports have shown that AhR activity in the absence of exogenous agonists or presence of the prototypical ligand 2,3,7,8-tetrachlorodibenzo- p -dioxin can affect G 1 phase progression in cultured cells. (aspetjournals.org)
Progression through the cell cycle is driven by the oscillating activity of Cyclin Dependent Kinases (CDKs). (intechopen.com)
Testing the activity of PLK4 in fruit flies revealed that it plays a similar role as in human cells. (elifesciences.org)
Given that autophagy is initiated upon suppressed mTORC1 activity, a protective autophagy in Ras-transformed cells with a high level of mTORC1 activity appears to be mTORC1-independent [ 20 , 21 ]. (aging-us.com)

AKAP12 knockdown in benign meningioma cells SF4433 increases proliferation, cell cycle, migration, invasion, and confers an anaplastic profile. (nature.com)
By conducting an in vitro CRC cell line study through the knockdown of EGFR expression, we analyzed cell proliferation, colony formation and migration. (oncotarget.com)

Impaired ribosome biogenesis is known to activate a p53-dependent checkpoint in mature cell lines, which leads to an arrest of cells in G1-phase. (uni-muenchen.de)
In order to enter S phase, cells must sequentially activate CDK4/6 and CDK2. (shu.edu)

risk of esophageal squamous cell carcinoma. (who.int)
Arbyn M, Bergeron C, Klinkhamer P, Martin-Hirsch squamous cell carcinoma in a high-risk region in Iran. (who.int)

Anatomy21

Organisms11

Diseases23

Chemicals and Drugs96

Analytical, Diagnostic and Therapeutic Techniques and Equipment37

Phenomena and Processes71

Disciplines and Occupations1

Information Science4

Health Care15

Differentiation21

Arrest and apoptosis1

Checkpoints6

Blocks cell cycle progression2

Inducing cell cycle arrest1

Mitosis7

Cyclins7

Protein18

Transition7

Induces2

Regulate3

Tumorigenesis and progression3

Inhibitor6

Contribute to cancer development and progression2

Kinase5

Mitotic cell cycle1

Proteins12

Role in promoting p1

Inhibitors5

Genes5

Reduced cell proliferation1

Epithelial3

Mitochondrial1

Viability3

Growth16

Specificity1

Interphase1

Progenitor1

MRNA1

Downregulation1

Receptor4

Pathway6

Suppress1

Eukaryotic1

Mutations3

Distinct4

Tumor cells3

Halt2

Inhibits2

Cancer23

Functional1

Flow Cytometry2

Regulation1

Morphological3

Mantle Cell Lym3

Tightly regulated1

Activation4

Malignant3

Contrast3

Metastasis2

Mechanisms6

Bone marrow4

Invasiveness1

Occurs1

Activity4

Knockdown2

Activate2

Squamous2