Cells producing recombinant proteins. Medical search. Frequent questions

Recombinant protein therapeutics, vaccines, and plasma products have a long record of safety. (researchgate.net)
Therapeutic proteins (e.g., antibodies, vaccines, enzymes, cytokines, and growth factors) account for almost half of this market, followed by industrial proteins (e.g., technical enzymes) and research reagents (e.g., antibodies for protein detection and purification) ( Markets and Markets, 2017 ). (frontiersin.org)
Whether you are producing antibodies, recombinant proteins, vaccines or viral vectors for gene therapies, our cell line platforms can accelerate your upstream progress. (sigmaaldrich.com)
Although the assembled VLPs do not have S protein spikes, they could be developed as formulations that can improve the immunogenicity of vaccines including S antigens, and further could be used as platforms that can carry S antigens of concern for various mutations. (nature.com)
Over one hundred vaccines using varied approaches including protein subunit, viral vector, RNA, DNA, inactivated virus, attenuated virus, and virus like particles (VLPs) platforms are in clinical trials. (nature.com)
These protein subunits can assemble into virus particles without viral RNA genome in mammalian cells to study virus-cell entry 2 and to develop vaccines 3 . (nature.com)
Packed bed bioreactors are widely used in the biopharmaceutical industry to produce various therapeutic proteins, antibodies, and vaccines. (tutorialspoint.com)
Various techniques have been developed for targeting cancer cells: gene therapy, monoclonal antibodies (MAbs), antibody toxin conjugates, small-molecule inhibitors, antisense molecules, and tumor vaccines. (medscape.com)
Animal cell technology is an area of rapid expansion and one that produces a wide range of high-value products, including vaccines, recombinant proteins, drugs for cardiovascular, respiratory and immune diseases, and monoclonal antibodies. (imperial.ac.uk)
Vaccines, plasma derived products, and their recombinant analogues are excluded from the scope of this document. (who.int)
No food proteins like eggs or egg products, gluten, peanuts, tree nuts, nut products, or any nut byproducts (COVID-19 vaccines are not manufactured in facilities that produce food products). (cdc.gov)

For contributions to mammalian cell culture and microbial processes that produce recombinant proteins and antibodies. (nae.edu)
An alternative approach to engage T cells for cancer therapy are antibodies, which are bispecific for a surface target antigen on cancer cells, and for CD3 on T cells. (aacrjournals.org)
Here, we review the principle of a new class of bispecific antibodies called BiTE (for "bispecific T-cell engager") antibodies. (aacrjournals.org)
The concept of using such bispecific antibodies to engage cytotoxic T cells for cancer cell lysis was shown by Staerz and colleagues ( 1 ). (aacrjournals.org)
Likewise, bispecific antibodies for engagement of other cytotoxic immune cells were constructed, for instance, targeting Fcγ RI/CD64 on macrophages and Her2/neu or EGFR on tumor cells. (aacrjournals.org)
Pioneering work by Kufer and colleagues ( 6 ) showed that CD3/target antigen-bispecific antibodies of this particular design had an exceptionally high potency, and could likewise engage CD8 + and CD4 + T cells for redirected lysis of cancer cells at very low effector to target (E:T) ratios. (aacrjournals.org)
By using just one CD3-specific binding arm, such bispecific antibodies can monovalently bind to all T cells, but do so only with rather low affinity ( 7 ), which will not trigger T-cell signaling by CD3, unless the BiTE antibody is presented to the T cell in a multivalent fashion by a target cell ( 8 ). (aacrjournals.org)
VLPs are highly immunogenic and can induce elevated titers of neutralizing antibodies, even without adjuvants, thanks to the maintenance of the native conformation of viral proteins presenting repetitive epitopes to the host immune system cells. (nature.com)
Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies. (antikoerper-online.de)
Packed bed bioreactors can be used to produce a wide range of products, from monoclonal antibodies to recombinant proteins. (tutorialspoint.com)
Targeted molecular therapy, like therapy with monoclonal antibodies, gene therapy, and other therapies, has limited or nonexistent side effects on normal cells of the body, unlike present modalities such as surgery, chemotherapy, and radiotherapy. (medscape.com)
These antibodies detected recombinant protein but failed to detect protein in L. mexicana cell lysates. (gla.ac.uk)
Antibodies raised against a peptide of GPI8 also recognised recombinant GPI8, but not GPI8 in L. mexicana lysates. (gla.ac.uk)
A protein of 38-40 kDa, however, was detected in cell lysates of T. brucei with the antirecombinant GPI8 antibodies. (gla.ac.uk)
Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.com)
Mammalian cells are currently the preferred expression system for high-value bio-pharmaceutical products, in particular, monoclonal antibodies. (imperial.ac.uk)
Sera from residual specimens from NHANES 2001-2002 participants were tested for levels of IgG antibodies against cancer antigen (CA) 125, CA15.3 and human epididymal secretory protein 4 (HE4). (cdc.gov)
Antibodies One of the body's lines of defense ( immune system) involves white blood cells (leukocytes) that travel through the bloodstream and into tissues, searching for and attacking microorganisms and. (msdmanuals.com)

Glycosylation is an important feature for the stability of "therapeutic proteins," or proteins used in drugs. (cornellsun.com)
Hence, recapitulating the islet ECM in both isolated islets and stem-cell-derived β-cells is of high therapeutic value. (nature.com)
Therapy - Therapeutic types of biopharmaceuticals include recombinant protein therapy, antibody therapy, cell therapy, and gene therapy. (dksh.com)
Apo-2L or tumor necrosis factor (TNF)-related apoptosis ligand, TRAIL, is one of several members of the TNF family and due its selectivity towards transformed versus normal cells it bears the potential as a cancer therapeutic agent. (uni-marburg.de)
This guideline applies to well-established and well-characterized biotherapeutic products such as recombinant DNA-derived therapeutic proteins. (who.int)

Cells were transfected with a plasmid overexpressing BHMT and probed with a BHMT monoclonal antibody (Product # MA5-25055). (thermofisher.com)
The CHO cell line secretes recombinant factor IX into a defined cell culture medium that does not contain hormones, and the recombinant factor IX is purified by a chromatography purification process that does not require a monoclonal antibody step. (globalrph.com)
More than 25 years ago, monoclonal antibody and recombinant DNA technologies enabled the construction of antibody-based molecules that normally do not occur in nature. (aacrjournals.org)
CDNA was prepared from isolated RNA of hybridoma cells expressing monoclonal antibody for TRAIL-R2 and CD95 (APO-1/Fas) and used for PCR amplification of heavy and light variable regions (VH and VL) genes. (uni-marburg.de)
However, compared to the parental anti-APO-1 antibody the potential of this recombinant antibody was not increased. (uni-marburg.de)
This antibody is specific for Myc tagged proteins. (abcam.com)
Current understanding of mammalian cell cultures lies in the association of their metabolic behaviour to their antibody production output. (imperial.ac.uk)
The main objective of the project is to study the effects of hyperosmotic pressure on the antibody production process in the GS-NS0 cell line, with particular attention to the antibody production mechanism. (imperial.ac.uk)

Human CXCL9 Recombinant Protein is purified CXCL9 (MIG) produced in yeast. (immunochemistry.com)
Other systems are used in commercial processes but are less common, including insect cells, yeast, algae, and cell-free expression platforms ( Markets and Markets, 2017 ). (frontiersin.org)
The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. (antikoerper-online.de)
The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. (antikoerper-online.de)
A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. (antikoerper-online.de)
Since Okanagan Specialty Fruits in Summer-land, Canada, planted its first test apples in 2003, the array of foods modified in labs has expanded to include meatless burgers, made with soya protein produced by recombinant yeast, fish fillets grown from seafood stem cells, and mushrooms whose genomes have been edited with CRISPR technology. (scientificamerican.com)
Two of the proteins involved in this step have been identified in yeast and mammals. (gla.ac.uk)
The predicted protein shares 31% identity with yeast and human homologues. (gla.ac.uk)
Sequence comparison of L. mexicana, yeast and human GPI8 proteins identified two potential active site cysteine residues. (gla.ac.uk)

Full length recombinant protein of human Sorbitol Dehydrogenase (NP_003095) produced in HEK293T cells. (novusbio.com)
Full length recombinant protein of human Proteasome 19S S3 (NP_002800) produced in HEK293T cells. (novusbio.com)
This is a Recombinant Protein Lysate produced in HEK293T cells. (innov-research.com)

Protein production Gene expression Protein purification Host cell protein Young CL, Britton ZT, Robinson AS (May 2012). (wikipedia.org)
Market growth has been supported by advances in recombinant protein production technologies, including the engineering of expression hosts, the optimization of upstream cultivation (e.g., bioreactor design, nutritional, and physical parameters), and the development of more efficient protein extraction and purification methods. (frontiersin.org)
LP3 is fully equipped for recombinant protein production in E. coli and insect cells (BEVS) and protein purification using state of the art chromatography systems. (lu.se)
LP3 and PPS offer services for the entire process chain of production, purification, characterization, protein crystallisation and protein structure determination and structure refinement, or each individual step in the chain. (lu.se)

Our recombinant cell culture supplements include albumin, transferrin, insulin, trypsin, NAO growth factors and transport proteins for production of mAbs and recombinant proteins, as well as cell and gene therapies. (sigmaaldrich.com)
For the company, all of these data demonstrate the potential for clinical use both as a monotherapy and in combination with insulin injections and/or other therapies targeting pancreatic beta cells. (investincotedazur.com)
Literature review: This review summarizes current knowledge, barriers, and challenges in the clinical use of adult stem cells, scaffolds, and growth factors for the development and evaluation of regenerative endodontic therapies. (bvsalud.org)
This explains why researchers are now focusing attention on developing stem cell therapies using postnatal stem cells donated by the patients themselves or their close relatives. (bvsalud.org)

Recent studies have shown that it may be possible to produce recombinant lysosomal proteins with microorganisms such as Escherichia coli and Saccharomyces cerevisiae. (wikipedia.org)
His group focuses on understanding the functions and structures of naturally existing protein machinery and creating microorganisms with artificial proteins machinery. (cornellsun.com)
Bioreactors are an essential part of bioprocessing, where they are used to cultivate microorganisms or cells and produce desired products. (tutorialspoint.com)
These reactors have unique design features that make them ideal for the cultivation of cells or microorganisms. (tutorialspoint.com)
The matrix provides a surface area for cells or microorganisms to attach and grow and also serves as a support for the cells or microorganisms. (tutorialspoint.com)
The cells or microorganisms are immobilized in the matrix and are not suspended in the liquid medium. (tutorialspoint.com)
In a batch mode, the medium is added to the bioreactor at the beginning of the process, and the cells or microorganisms are allowed to grow until the desired product is produced. (tutorialspoint.com)
Packed bed bioreactors work by immobilizing cells or microorganisms in a solid matrix and providing optimal growth conditions for their growth and metabolism. (tutorialspoint.com)
The matrix provides a large surface area for the cells or microorganisms to attach and grow, allowing for high cell densities and efficient mass transfer. (tutorialspoint.com)
The growth of cells or microorganisms in packed bed bioreactors can be influenced by various factors, including the flow rate of the medium, the nutrient concentration, and the oxygen concentration. (tutorialspoint.com)
The medium flow rate affects the rate of nutrient delivery to the cells or microorganisms and the removal of waste products. (tutorialspoint.com)
The nutrient concentration affects the growth rate of the cells or microorganisms and their metabolism. (tutorialspoint.com)
Packed bed bioreactors can be used to cultivate microorganisms that produce ethanol and biodiesel from renewable resources, such as sugar cane and algae. (tutorialspoint.com)

Most recombinant proteins are currently produced in prokaryotic cells (mainly the bacterium Escherichia coli ) and a small number of well-characterized mammalian cell lines, such as Chinese hamster ovary (CHO) cells. (frontiersin.org)
Recombinant COMT was produced in Escherichia coli up to 10% of total bacterial protein after the induction of the T7 RNA polymerase gene with isopropyl-β-d-thiogalactopyranoside. (lu.se)

23, 2020 The mammalian cell lines that are engineered to produce high-value recombinant-protein drugs also produce unwanted proteins that push up the overall cost to manufacture these drugs. (sciencedaily.com)
Further, VLPs have the advantage that, contrary to inactivated or attenuated viruses that must be prepared in mammalian cell lines, they can be produced in heterologous systems, such as bacteria, yeasts, insect cells, or plants. (nature.com)

Founded in 2020 based on the research work of Patrick Collombat, one of the leading scientists in the field of pancreatic beta cell regeneration, DiogenX is developing first-in-class recombinant proteins designed for the treatment of type 1 diabetes. (investincotedazur.com)

In many cases, recombinant human proteins have replaced the original animal-derived version used in medicine. (wikipedia.org)
The prefix "rh" for "recombinant human" appears less and less in the literature. (wikipedia.org)
Bulk Order Inquiry for Recombinant Human CXCL9 (MIG) ------- (please add any order requirements, including desired quantity, timing, etc. (immunochemistry.com)
The human CXCL9/MIG protein can be used in cell culture, as a CXCL9/MIG ELISA Standard, and as a Western Blot Control. (immunochemistry.com)
IDO1 is widely expressed in a variety of human tissues as well as in macrophages and dendritic cells (DCs). (thermofisher.com)
IL21 Protein LS-G3653 is a Recombinant Human IL21 produced in E. coli. (lsbio.com)
Determined by its ability to stimulate the proliferation of human ANBL-6 cells. (lsbio.com)
No human or animal proteins are added during any stage of manufacturing or formulation of RIXUBIS. (globalrph.com)
From efficient biosynthesis of complex protein therapeutics to discovery of new modes of drug delivery, DeLisa seeks to impact a wide range of problems in human health, specifically those that cannot be solved using natural systems. (cornellsun.com)
Thus, human endocrine cells are not in direct contact with the vascular BM components but with the invaginated peri-islet membrane. (nature.com)
Interferon beta-1a (Avonex) is a recombinant form of a physiologic human type I interferon important for antiviral, immunomodulatory and antiproliferative effects. (clinimmsoc.org)
Most companies in this industry produce medications for human illnesses. (dksh.com)
Besides the high apoptosis-inducing activity on cancer cells, no toxicity in mice and on freshly isolated human hepatocytes of either IZ-TRAIL alone or in combination with diverse chemotherapeutic drugs was observed. (uni-marburg.de)
Human ADAM19 Protein Lysate 20ug from Innovative Research is provided as a Lyophilized powder. (innov-research.com)
During preclinical research, DiogenX has achieved a preliminary proof of concept with a significant increase in functional human beta cells, which are responsible for insulin production. (investincotedazur.com)
Recombinant Human KRT6C aa. (creativebiomart.net)
To produce sufficient amounts of recombinant catechol-O-methyltransferase (COMT) for structural and functional studies the coding regions of the rat liver and human placental COMT genes have been introduced into a bacterial expression vector pKEX14. (lu.se)
The possible donor-host rejection of human ES cells is another concern 3 . (bvsalud.org)
To date, four types of human dental stem cells have been isolated and characterized: (i) dental pulp stem cells (DPSCs) 12 (ii) stem cells from exfoliated deciduous teeth (SHED) 21 (iii) stem cells from apical papilla (SCAP) 31 (iv) periodontal ligament stem cells (PDLSCs) 28 . (bvsalud.org)

Proteins are therefore molecules of great complexity and near infinite diversity, making them suitable for many different applications. (frontiersin.org)
Protein molecules play a critical role in every living creature, as they participate in making structures in the cell and working as the liaison in signaling between molecules. (cornellsun.com)
Glycosylation is an essential process which produces sugars, which are attached to organic molecules, such as proteins and lipids. (cornellsun.com)
Together these proteins shorten the end of the mRNAs, preparing the molecules for degradation. (elifesciences.org)
Protein labeling is used by researchers in a variety of fields to help them understand how these important molecules affect the normal functioning of cells. (sciencedaily.com)

All tissues originate from stem cells. (bvsalud.org)
A stem cell is commonly defined as a cell that has the ability to continuously divide and produce progeny cells that differentiate (develop) into various other types of cells or tissues. (bvsalud.org)
Postnatal stem cells have been sourced from umbilical cord blood, umbilical cord, bone marrow, peripheral blood, body fat, and almost all body tissues, including the pulp tissue of teeth 8 . (bvsalud.org)
No tissues like aborted fetal cells, gelatin, or any materials from any animal. (cdc.gov)

Critical for the development and production of biotherapeutic products, cell culture for manufacturing requires scalable products that are traceable and pure yet economically sound. (sigmaaldrich.com)

RIXUBIS [Coagulation Factor IX (Recombinant)] is a purified protein produced by recombinant DNA technology. (globalrph.com)
Each vial contains nominally 250, 500, 1000, 2000 or 3000 international units of recombinant coagulation factor IX. (globalrph.com)

The function of a protein is determined by the number and sequence of amino acids, which controls the three-dimensional structure of the resulting folded polypeptide. (frontiersin.org)
A gene encodes the amino acid sequence of a protein in a linear sequence of 3-base triplet codons that correspond in linear order to the amino acid sequence of the protein. (patentdocs.org)
The entire gene, junk and coding sequence, is transcribed into RNA by the cell and then the junk pieces are 'spliced out,' leaving the coding sequence (termed a messenger RNA or mRNA) that is used to produce the protein. (patentdocs.org)
The recombinant protein is glycosylated and is identical to the native protein in sequence (MW 22.5kDa). (clinimmsoc.org)

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. (origene.com)
This allows the cell to switch networks of related genes off together without affecting the mRNAs it still needs. (elifesciences.org)
This could reveal genes that work together, helping to unravel their roles inside cells. (elifesciences.org)
Genes have been described, properly, as the instructions for producing proteins. (patentdocs.org)
However, in humans (and all other animals) almost all genes are interrupted by 'junk' DNA that does not encode protein (or, at least, the protein the gene encodes). (patentdocs.org)
In contrast, culturing on Laminin-421 induced the expression of β-cell genes, including Ins1 , MafA , and Glut2 , and significantly improved glucose-stimulated insulin secretion. (nature.com)
This task can be accomplished by replacing tumor suppressor genes that have been lost or mutated, selectively inserting genes that produce cytotoxic substances, or modifying the immune system to destroy the tumor cells. (medscape.com)

Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. (novusbio.com)
Many eukaryotic cell surface proteins are attached to the plasma membrane by glycosylphosphatidylinositol (GPI) anchors. (gla.ac.uk)

RNA-binding proteins contribute to specificity by interacting with both Ccr4-Not and target mRNAs, but this is not fully understood. (elifesciences.org)
These are capable of connecting any kind of cytotoxic T cell to a cancer cell, independently of T-cell receptor specificity, costimulation, or peptide antigen presentation. (aacrjournals.org)

Since 1994, ImmunoChemistry Technologies has been producing top quality reagents, kits, and services for cell viability and ELISA applications in a broad range of research areas. (immunochemistry.com)

Cystatins are proteins that inhibit cysteine proteases. (wikipedia.org)

Recently, a book chapter with protocols for the Baculovirus Expression Vector System in insect cells was published by LP3. (lu.se)
Lund Protein Production Platform (LP3) has recently published its standard operating procedures for recombinant protein expression using the Baculovirus Expression Vector System (BEVS) in insect cells. (lu.se)
LP3 is the only academic protein production platform in Sweden providing specialisation in protein production using the baculovirus expression vector system (BEVS, insect cells) and perdeuteration of biological macromolecules. (lu.se)

Receptor for Advanced Glycation End-Products ( Ager gene) encodes RAGE, a transmembrane protein belonging to the immunoglobulin (Ig) superfamily of receptors. (elifesciences.org)

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. (origene.com)
β-Cells depend on the islet basement membrane (BM). (nature.com)
The peri-islet membrane, which encapsulates the islets, separates the endocrine cells from the exocrine pancreas and serves as a barrier from immune cell infiltration to the islets. (nature.com)
Here, we demonstrate that non-infectious SARS-CoV-2 VLPs can be successfully assembled by co-expressing three important viral proteins membrane (M), envelop (E) and nucleocapsid (N) in plants. (nature.com)
SARS-CoV-2 is composed of four essential structural proteins: the spike (S) glycoprotein, membrane (M) protein, envelope (E) protein, and nucleocapsid (N) protein, as well as multiple accessory and non-structural proteins 1 . (nature.com)
For crystallisation, the facility is equipped with state-of-the-art nanolitre pipetting equipment with capability to handle lipidic cubic phases for membrane proteins, as well as a plate hotels with the capacity to store and automatically image crystallisation plates. (lu.se)

Lysosomal proteins: Lysosomal proteins are difficult to produce recombinantly due to the number and type of post-translational modifications that they have (e.g. glycosylation). (wikipedia.org)
Consequently, as the most common expression host for such proteins, E.coli's ability to produce proteins that can be modified for glycosylation is advantageous. (cornellsun.com)
Glycosylation) which are affecting the protein of interest. (boku.ac.at)

The tyrosinase‐induced polymerization of peptides offers facile access to artificial mussel foot protein analogues. (wikipedia.org)

However, tumor-specific T-cell responses are difficult to mount and sustain in cancer patients, and are limited by numerous immune escape mechanisms of tumor cells selected during immunoediting. (aacrjournals.org)
The specific binding of the scFv-anti-TRAIL-R2 fragment to TRAIL-R2 expressing tumor cells was confirmed by FACS analysis. (uni-marburg.de)
Only minimal lytic activity of the scFv-anti-TRAIL-R2 fragment on a variety of tumor cells could be detected. (uni-marburg.de)
The anti-tumoral efficacy of IZ-TRAIL alone or in combination with different chemotherapeutic drugs was evaluated on a variety of tumor cells. (uni-marburg.de)
Therefore, in contrast to non-tagged versions of TRAIL this recombinant IZ-TRAIL shows high activity against tumor cells and, at the same time, it is not toxic to primary cells. (uni-marburg.de)

Our off-the-shelf and customizable bioprocessing cell culture media (CCM) products enhance productivity in upstream mAb, vaccine, gene/cell therapy processes. (sigmaaldrich.com)
Models of animal cell culture systems have a wide range of potential applications, such as analysis and prediction of experimental results, optimisation of culture conditions for prolonged viability, and perhaps most importantly, the investigation of fundamental metabolic processes and their subsequent elucidation. (imperial.ac.uk)
The integration of these three research tools represents a unique, novel, and interdisciplinary approach to addressing the complicated research and industrial problem of model-based control and optimisation of mammalian cell culture processes. (imperial.ac.uk)

To decipher the molecular basis for the variation in hlgCB expression and protein production among strains, different regulation levels were analyzed in representative clinical isolates and reference strains. (bvsalud.org)
Methods and Materials: We recently showed that differential susceptibility to PQ neurotoxicity in four inbred mouse strains from the family of BXD recombinant inbred strains is associated with the increase in iron concentration in the ventral midbrain - the area containing both the SNc and ventral tegmentum. (cdc.gov)
We also studied genetic differences among 10 BXD recombinant inbred mouse strains to MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine), a proneurotoxicant (the active agent is the metabolite, MPP+ produced in astrocytes) used to model the pathophysiology of Parkinson's disease. (cdc.gov)

0.0-7.5) virus-mediated insect cell expression system. (cdc.gov)
Over the last three decades, the expression of recombinant proteins in plants and plant cells has been promoted as an alternative cost-effective production platform. (frontiersin.org)
Here, we discuss the strengths of plant expression systems for specific applications, but mainly address the bottlenecks that must be overcome before plants can compete with conventional systems, enabling the future commercial utilization of plants for the production of valuable proteins. (frontiersin.org)
Even so, plants as an alternative expression platform offer unique advantages, particularly when target proteins are difficult to produce in conventional systems, require specific qualitative properties such as particular glycan profiles, and/or must be produced on a larger scale in response to urgent demand. (frontiersin.org)
Whereas α2 laminins promoted islet cell clustering, they did not affect gene expression. (nature.com)
The recombinant proteins were produced by transformation of E.coli with the generated expression leucine zipper plasmids. (uni-marburg.de)
But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. (antikoerper-online.de)
3) Episomal re-expression of GPI8 restored GP63 to the cell surface. (gla.ac.uk)
This unexpected finding suggests that by preserving the expression of RAGE protein (in a dominant-negative form), Ager AHA/AHA mouse might represent a cleaner genetic model to study physiological roles of RAGE in vivo compared to Ager −/− mice. (elifesciences.org)
It appears to produce its antiangiogenic effect by down-regulating the expression of basic fibroblast growth factor (bFGF) in the rapidly proliferating vascular lesion. (medscape.com)

LP3 is colocalised with and closely collaborates with the DEMAX platform of the ESS on production of deuterium labeled biomolecules (proteins, lipids) for neutron scattering experiments and the development of methods for crystal growth for neutron crystallography. (lu.se)

More than 300 protein-based medicines have been approved in the USA and Europe, and proteins account for almost a third of all pharmaceuticals in development ( Walsh, 2018 ). (frontiersin.org)

RECOTHROM thrombin is available as 5000-unit and 20,000-unit vials of sterile recombinant topical thrombin lyophilized powder for solution. (drugs.com)
Our custom cell culture product design experts can offer advice and support as you scale your bioprocess from clinical to commercial supply, assisting with liquid products, dry powder products, selection of raw materials, quality testing, flexible product packaging, and product documentation. (sigmaaldrich.com)

Biopharmaceuticals are medical therapeutics or drugs that have been synthesized or extracted from a biological source, such as living cells or organisms. (dksh.com)

Ultimately, his works in this topic will lead to "humanizing" proteins, which are produced by E.coli cells. (cornellsun.com)
Glu163~Gly482 (Accession # P48668)) fused with N-terminal His & T7 tag was produced in E. coli cells. (creativebiomart.net)
Both enzymes were purified from E. coli cells and partially characterized by determining their specific activity, apparent molecular weight and pI. (lu.se)

Protein crystallization is a key assay for structural studies of proteins. (gracebio.com)
Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. (creativebiomart.net)

Pancreatic pericytes directly support β-cells through mostly unidentified secreted factors. (nature.com)
Here, we show that pericytes produce multiple components of the mouse pancreatic and islet interstitial and BM matrices. (nature.com)
Compared to ECs, pancreatic pericytes produce significantly higher levels of α2 and α4 laminin chains, which constitute the peri-islet and vascular BM. (nature.com)
The main program of DiogenX aims to regenerate insulin-producing pancreatic beta cells using a recombinant protein that modulates the Wnt/β-catenin signaling pathway. (investincotedazur.com)
Type 1 diabetes is an autoimmune disease in which the insulin-producing pancreatic beta cells are destroyed by the immune system. (investincotedazur.com)

Deiters and Chin developed a special 21st amino acid that they added to cells that were specially engineered to incorporate this amino acid into the protein they wanted to study (there are normally only 20 amino acids). (sciencedaily.com)

This antiserum gave a similar immunofluorescence pattern in T. brucei to those of an epitope-tagged ribosomal protein, QM. (gla.ac.uk)

Lund Protein Production Platform (LP3) is a cross-faculty expert center and user facility of Lund University (LU). (lu.se)

These include both commercially available proteins (for example most of the enzymes used in the molecular biology laboratory), and those that are generated in the course specific research projects. (wikipedia.org)
Filgrastim is a natural man-made protein produced commercially by recombinant DNA technology, which stimulates production of white blood cells. (worldpharmanews.com)

We offer the industry's highest quality sterile filtered liquid capabilities, supplying ready-to-use cell culture media, buffers, CIP and SIP products from GMP facilities worldwide to optimize your biopharma production. (sigmaaldrich.com)

All NHANES serum biospecimens were tested at a 10-fold dilution and repeated at a 50-dold dilution if producing an outlier value in the specific antigen or albumin spots. (cdc.gov)

Many other proteins are used as diagnostics or research reagents. (frontiersin.org)

Plant cell culture was used to produce FDA-approved glycosylated lysosomal protein-drug, and additional drug candidates. (wikipedia.org)
Although plant systems are now gaining widespread acceptance as a platform for the larger-scale production of recombinant proteins, there is still resistance to commercial uptake. (frontiersin.org)
Nevertheless, some plant-derived proteins for research or cosmetic/pharmaceutical applications have reached the market, showing that plants can excel as a competitive production platform in some niche areas. (frontiersin.org)
There are also several platforms based on plants and plant cells, but these have not been included in the latest market studies, indicating they have not yet commanded a significant share of commercial protein production capacity. (frontiersin.org)
These plant-produced VLPs are capable of inducing protective immunity. (nature.com)
He found his answer in Australia, where researchers at the Commonwealth Scientific and Industrial Research Organisation had figured out how to delete a gene encoding an enzyme that causes plant cells to brown when exposed to oxygen. (scientificamerican.com)
The familiar leafy plant of Bryophytes is the sexual, gamete-producing (gametophyte) generation of their life cycle. (tripod.com)
E. coli, P. pastoris , Mammalian cells, Plant cells and in vitro by cell free synthesis. (lu.se)

We are deeply committed to providing you with robust products to meet your mammalian cell culture needs. (sigmaaldrich.com)
Our highly validated, optimized products, including upstream chemicals, supplements, chemically defined cell culture media, bioreactors and clarification filters, coupled with decades of expertise, provide convenient solutions to even the most complex challenges. (sigmaaldrich.com)
CDC, our planners and presenters wish to disclose they have no financial relationships with ineligible companies whose primary business is producing, marketing, selling, reselling, or distributing healthcare products used by or on patients. (cdc.gov)
If we are honest in defining what are actually 'products of nature,' then they must by definition be produced by nature. (patentdocs.org)
RAGE binds a wide array of ligands including advanced glycation end products, amyloid β-peptide, S100 family proteins, and high mobility group Box 1 (HMGB1). (elifesciences.org)

Homo sapiens programmed cell death 1 (PDCD1), mRNA. (origene.com)
When a cell needs to make a particular protein, it first copies the instructions from the matching gene into a molecule known as a messenger RNA (or an mRNA for short). (elifesciences.org)
The more mRNA copies it makes, the more protein it can produce. (elifesciences.org)
A simple way to control protein production is to raise or lower the number of these mRNA messages, and living cells have lots of ways to make this happen. (elifesciences.org)
The ability to destroy specific mRNA messages is critical for cell survival. (elifesciences.org)

However, the use of cell culture to produce recombinant proteins is still susceptible to contamination with viruses. (researchgate.net)

Proteins are also widely used in industry, including enzymes used to manufacture textiles and chemicals and to process food and feed. (frontiersin.org)
Packed bed bioreactors can be used to produce a wide range of food ingredients and additives, such as enzymes for cheese production, flavours for beverages, and preservatives for baked goods. (tutorialspoint.com)

In addition to providing biomechanical support to the islets, ECM components are required for β-cell development, proliferation, survival, and proper insulin secretion. (nature.com)

Unlike traditional nonsteroidal anti-inflammatory drugs and selective cyclooxygenase-2 inhibitors, AK106-001616 reduced prostaglandin E 2 (PGE 2 ) and leukotriene B 4 (LTB 4 ) production by stimulated cells. (aspetjournals.org)
Our ready-to-use, off-the-shelf cell line systems greatly reduce the time, cost, and risk of developing new cell lines for production. (sigmaaldrich.com)
A transfection-based solution to viral vector production using a suspension cell line, chemically defined medium, and a process with proven performance at scale. (sigmaaldrich.com)
Thus, we hypothesized that pericytes regulate β-cells through the production of BM components. (nature.com)
Recombinant GPI8 was produced in E. coli and used to inoculate rabbits for the production of anti-serum. (gla.ac.uk)
As such, we provide services and make equipment available in the areas of recombinant protein production, crystallisation, biophysical characterization, and structure determination. (lu.se)

In other words, they are medicines derived from living systems and produced using biotechnology. (dksh.com)
Sandoz develops, produces and markets these medicines along with pharmaceutical and biotechnological active substances and anti-infectives. (worldpharmanews.com)

Envelope protein of the hepatitis B virus marketed as Engerix-B by SmithKline Beecham HPV Vaccine proteins Polyphenol oxidases (PPOs): These include both catechol oxidases and tyrosinases. (wikipedia.org)
After the body produces an immune response, it gets rid of all of the vaccine ingredients just as it would discard any information that cells no longer need. (cdc.gov)

There is increasing evidence that T cells are able to control tumor growth and survival in cancer patients, both in early and late stages of the disease. (aacrjournals.org)
The HER (erbB) family of transmembrane receptor tyrosine kinases is one of the cytostatic targets in tumor cell growth and survival. (medscape.com)
It remains to be established if GPI-anchored proteins are required for survival in the sandfly. (gla.ac.uk)
Opinions are embraced and defended, but transfusion of red blood cells has not reliably demonstrated increased survival, other than in 2 specific populations, as follows: (1) those with active hemorrhage, and (2) those with active cardiac ischemia. (medscape.com)

This is particularly notable because, in these species, 20E is also produced by females after every blood meal, whereby 20E drives the oogenetic cycles (reviewed in ref. 8 ). (nature.com)

E.coli is used widely in biotechnology as a host cell to produce recombinant proteins. (cornellsun.com)

The protocols for crystallization of protein are challenging due to the stringent requirement for pure samples and control of environmental conditions during the crystallization process. (gracebio.com)

Anatomy16

Organisms28

Diseases1

Chemicals and Drugs95

Analytical, Diagnostic and Therapeutic Techniques and Equipment52

Phenomena and Processes72

Disciplines and Occupations1

Technology, Industry, Agriculture4

Information Science4

Health Care5

Vaccines11

Antibodies18

Therapeutic5

Antibody8

Yeast9

HEK293T3

Purification4

Therapies4

Microorganisms13

Escherichia2

Mammalian cell lines2

20201

Human19

Molecules5

Tissues4

Biotherapeutic products1

Coagulation2

Sequence4

Genes7

Eukaryotic2

Specificity2

ELISA1

Cysteine1

Insect cells3

Receptors1

Membrane6

Glycosylation3

Analogues1

Tumor cells5

Processes3

Strains3

Expression10

Lipids1

20181

Powder2

Therapeutics1

Coli cells3

Structural2

Pancreatic5

Amino1

Ribosomal1

Lund Protein Production Platform1

Commercially2

Sterile1

Albumin1

Reagents1

Plant8

Products5

MRNA5

Susceptible1

Enzymes2

Insulin1

Production6

Medicines2

Vaccine2

Survival4

Species1

Biotechnology1

Protocols1