Cells of differentiation fetal dermal cells fetal tissue research. Medical search. Frequent questions

Using Foxn1-/- and Foxn1+/+ mice, we demonstrated that epidermally expressed Foxn1 regulates dWAT development and defines the adipogenic capacity of dermal fibroblasts. (bvsalud.org)
Furthermore, Foxn1 activity strengthens adipogenic processes through Bmp2 and Igf2 signaling and regulates lipid metabolism in differentiated dermal fibroblasts. (bvsalud.org)
iPS were generated via a platelet lysate or fetal bovine serum-based culturing protocol from matched dermal, buccal and gingival human fibroblasts, isolated from healthy donors ( n = 2) after informed consent, via episomal plasmid transfection. (biomedcentral.com)
Furthermore, oral fibroblasts generally required more attempts for successful iPS generation as opposed to dermal fibroblasts. (biomedcentral.com)
The oral fibroblasts generally proved to be more difficult to reprogram than dermal fibroblasts. (biomedcentral.com)
Xeno-free dermal, buccal and gingival fibroblasts can successfully generate iPS with a comparable genotype/phenotype to their xenogenic counterparts. (biomedcentral.com)
Conditioned medium from adipose derived stem cells (ADSC-CM) stimulates both collagen synthesis and migration of fibroblasts, and accelerates wound healing in vivo. (indexindex.com)
After ADSC-CM addition, collagen type I, type III, HAS and hyaluronic acid (HA) expressions on human dermal fibroblasts (HDFs) were evaluated. (indexindex.com)
Moreover, proliferating cell nuclear antigen (PCNA) and CD31 showed co-localization with α-SMA, suggesting the differentiation of hBMSCs into epithelial cells and myofibroblasts/fibroblasts. (ijbs.com)
In this study, employing iPS technology, we derived and characterized iPSCs from dermal fibroblasts of an 82-year-old female patient affected by sporadic AD. (biomedcentral.com)
Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. (thermofisher.com)
Herein, we introduce a cardiac-mimetic cell-culture system that resembles the microenvironment in the heart and provides interactions with cardiomyocytes and electrical cues to the cultured fibroblasts for direct cardiac reprogramming. (thno.org)
The human neonatal dermal fibroblasts containing cardiac transcription factors were plated on the membrane and cultured with the murine cardiomyocyte in the presence of the electric stimulus. (thno.org)
Subsequently, the scaffold was bioconjugated with apelin-13 using the EDC-NHS linker and acellular scaffolds were recellularized using fibroblasts, endothelial cells, and smooth muscle cells. (biomedcentral.com)
In the present study, we show that let-7c miRNA is decreased in heat-denatured fibroblasts and that inhibiting let-7c expression leads to the increased proliferation and migration of dermal fibroblasts, whereas the overexpression of let-7c exerts an opposite effect. (molcells.org)

6. Mogami H, Keller PW, Shi H, Word RA (2014) Effect of Thrombin on Human Amnion Mesenchymal Cells, Mouse Fetal Membranes, and Preterm Birth. (yoproteins.eu)
We further discuss how injuries alter tissue environments and how this primes mononuclear phagocytes to enforce this particular environment, for example, to support host defense and pathogen clearance, to support the resolution of inflammation, to support epithelial and mesenchymal healing, and to support the resolution of fibrosis to the smallest possible scar. (hindawi.com)
4 HFSCs are located in the bulge region of the follicle and they interact with mesenchymal SCs (MSCs) located in the dermal papilla (DP). (benitonovas.com)
Mesenchymal stem/stromal cells (MSCs)‐based therapy brings the reassuring capability to regenerative medicine through their self‐renewal and multilineage potency. (biomedcentral.com)
After that, Kaplan firstly proposes the term "mesenchymal stem cells," which are cells isolated from fully developed bone marrow (BM) that can usually differentiate into several types of mesenchymal origin cells [ 4 ]. (biomedcentral.com)
Mesenchymal stromal cells (MSCs) expanded in vitro have been proposed as a potential therapy for congenital or acquired skin defects in pediatrics. (biomedcentral.com)
The aim of this pre-clinical study was to investigate the effects of intradermal injections of MSC in experimental cutaneous wound repair comparing allogeneic and autologous adipose stem cells (ASCs) and autologous bone marrow-mesenchymal stromal cells (BM-MSCs). (biomedcentral.com)
Mesenchymal stromal cells were in vitro expanded from adipose and BM tissues of young female New Zealand rabbits. (biomedcentral.com)
Fracture Healing in Diabetes Fracture repair entails recruitment of mesenchymal stem cells after which differentiation to chondrocytes that type cartilage (see Chapter 12). (dnahelix.com)
While numerous teams continue to refine and expand the role of bone marrow and cord blood stem cells for their vanguard uses in blood and immune disorders, many others are looking to expand the uses of the various types of stem cells found in bone marrow and cord blood, in particular mesenchymal stem cells, to uses beyond those that could be corrected by replacing cells in their own lineage. (biomedcentral.com)
There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)
Mariano García Arranz has the following conflict of interest: MGA is inventor on 2 patents entitled "Identification and isolation of multipotent cells from non-osteochondral mesenchymal tissue" (10157355957US) and "Use of adipose tissue-derived stromal stem cells in treating fistula" (US11/167061). (wjgnet.com)
6,7 Mesenchymal stem cells (MSCs) have also been isolated from other sources, such as bone marrow, 8 the placenta, 9 muscle, 10 or blood. (jcadonline.com)
Mesenchymal stem cell transplantation and DMEM administration in a 3NP rat model of Huntington's disease: morphological and behavioral outcomes. (allergy-link.com)
Transplantation of mesenchymal stem cells (MSCs) may offer a viable treatment for Huntington's disease (HD). (allergy-link.com)

The pathological potential of human astroglia in Alzheimer's disease (AD) was analysed in vitro using induced pluripotent stem cell (iPSC) technology. (nature.com)
This work not only presents a novel model to study the mechanisms of human astrocytes in vitro , but also provides an ideal platform for further interrogation of early astroglial cell autonomous events in AD and the possibility of identification of novel therapeutic targets for the treatment of AD. (nature.com)
Following in vitro exposure to CTX, hBMSCs showed decreased cell growth in a dose- and time-dependent manner, accompanied by increased expressions of collagen-I/III, and CD31. (ijbs.com)
The stem cell field witnessed a genuine breakthrough when a combination of solely four transcription factors ( Oct3 / 4 , Sox2 , Klf4 and c-Myc, OSKM ) proved enough to revert, in vitro, the differentiated status of a variety of cell types back to pluripotency, giving rise to so-called induced pluripotent stem (iPS) cells. (springer.com)
In 1995, an Editorial in Gastroenterology stated: "… because of differences in relative abundance and ease of study, ductal cells have received less scrutiny than acinar cells… One way to overcome the problems inherent with studies of acutely isolated duct cells is to culture them in vitro… However, whereas monolayers of duct cells in culture have been produced, functional studies have not yet been successful" (7). (pancreapedia.org)
The neuronal cells expressed numerous genes associated with sub-regions within the brain thus suggesting the usefulness of our in-vitro model. (biomedcentral.com)
Rabbit ASCs were isolated and expanded in vitro with relative abundance, cells expressed typical surface markers (CD49e, CD90 and CD29). (biomedcentral.com)
These multipotent cells with innate self-renewal capacity can be in vitro expanded without losing their differentiation potential. (biomedcentral.com)
Therefore, the implantation of human cells reprogrammed in vitro represents an alternative strategy [ 16 - 19 ]. (thno.org)
In vitro toxicity data of these metabolites derived in the development of validated and accepted in vitro and in silico embryonic stem cell test were used as input in the PBK model to extrapolate in vitro concentration-response curves to predicted approaches is urgently needed. (cdc.gov)

Therefore, we aimed to compare the efficiency of using humanized culture conditions, specifically human platelet lysate to fetal bovine serum, for iPS generation from different sources, and to evaluate their stemness. (biomedcentral.com)
Intracel, Frederick, MD), 10 ng/mL salt selenite (Sigma-Aldrich), 0.01 mg/mL transferrin (BD Biosciences), 0.01 mg/mL insulin (Sigma-Aldrich), 10% Kl fetal bovine serum (Mediatech), 100 U/mL penicillin, and 100 g/mL streptomycin (Mediatech). (cancerhugs.com)
HUVECs had been cultured in Y12K formulated with 2 millimeter L-gluatmine, 2.5 g/L sodium bicarbonate, 0.1 mg/mL heparin (Sigma-Aldrich), 0.03 mg/mL ECGS, 10% fetal bovine serum, 100 U/mL penicillin, and 100 g/mL streptomycin. (cancerhugs.com)
NIH3Testosterone levels3 mouse fibroblast cells had been cultured in Dulbeccos customized Eagles moderate (DMEM) formulated with 4 mM L-glutamine, 1.5 g/L sodium bicarbonate, 4.5 g/L glucose (Mediatech), 10% fetal bovine serum, 100 U/mL penicillin, and 100 g/mL streptomycin. (cancerhugs.com)
ARPE-19 cells at passages p22 to p28 were cultured on filters for up to 3 months in DMEM/F12 or DMEM media with or without pyruvate and 1% fetal calf serum . (allergy-link.com)

Activation of muscle-specific genes in pigment, nerve, fat, liver, and fibroblast cell lines by forced expression of MyoD. (springer.com)
The current research was hence undertaken to assess the differential results of hydrogel modulus on cell growth, morphology, and gene phrase of vascular endothelial, simple muscles, and fibroblast cells, to offer even more complete details relevant to the design of healing biomaterials for dealing with cardiac disease. (cancerhugs.com)

Since that Editorial, we have been able to culture and propagate non-transformed and well-differentiated pancreatic duct epithelial cells (PDEC), obtained from the main pancreatic duct of a dog, and have used them as model for pancreatic ductal secretion. (pancreapedia.org)
6), by treating a normal human gallbladder with 0.5% trypsin / 0.2% EDTA for 1 hour to dissociate myofibroblasts (as well as mucosal epithelial cells). (pancreapedia.org)
Because gallbladder myofibroblasts attach and grow on plastic culture plates while epithelial cells do not, HGBMF were selected by culturing them on 60 mm plastic culture plates (seeding density ~ 10 5 cells per dish) in MEM. (pancreapedia.org)
Recent investigations have shown that the RON receptor is expressed in various cell types including epithelial cells (23) and that MSP-mediated effects on epithelial cells are integrin-dependent (23, 24). (123dok.org)
The immunogen used to generate the G8.8 antibody was the TE-71 thymic epithelial cell line. (thermofisher.com)
CD326 is expressed on the majority of epithelial cells, and is considered a pan-carcinoma antigen. (thermofisher.com)
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. (thermofisher.com)
Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. (thermofisher.com)
Loss of tissue is then repaired in a complex process that involves tissue-resident immune, stromal, and epithelial cells, as well as infiltrating immune cells [ 2 ]. (biomedcentral.com)
This thinning is also primarily a dermal change, with loss of elastic fibers, epithelial appendages, and ground substance. (medscape.com)

24 - 26 ECs may provide signals for BMP receptor activation in DPCs similar to those signals that promote survival of MSCs in human embryoid bodies composed of multipotent cells. (benitonovas.com)
Although there is no particular quantitative assay to provide MSCs identification in mixed cells population [ 8 ], the International Society for Cellular Therapy (ISCT) has provided minimum principles to determine MSCs. (biomedcentral.com)
However, natural MSCs in vivo survival and their biological effects on tissue recovery decrease with long-term cultivation called aging and also injected cells demonstrate poor targeted migration [ 21 ]. (biomedcentral.com)
MSCs were characterized for plastic adhesion, surface markers, proliferation and differentiation capacity. (biomedcentral.com)
When an adequate number of cells (ASCs 10 × 10 6 and BM-MSCs 3 × 10 6 , because of their low rate of proliferation) was reached, two skin wounds were surgically induced in each animal. (biomedcentral.com)
MSCs have been isolated from various tissues such as bone marrow (BM), umbilical cord blood, skeletal muscle and brain. (biomedcentral.com)
For instance ASCs show a higher proliferative capacity and retain differentiation potential for a longer period in culture compared with human BM-MSCs. (biomedcentral.com)
This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)
The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
11 However, the greater the abundance of adipose tissue in the human body, the significantly higher the yield of MSCs is compared to other tissues. (jcadonline.com)

In order to eliminate the interference from the transplanted cells themselves, fetal stem cells were used for transplantation because the proliferation and differentiation potentials of fetal stem cells are superior to the adult ones ( 8 ). (ijbs.com)

The present study performed on abdominal human intact and scar skin focused on determining gender differences in extracellular matrix (ECM) composition, dermal white adipose tissue (dWAT) accumulation, and Foxn1 expression as a part of the skin response to injury. (bvsalud.org)
Extracellular matrix scaffolds derived from porcine small intestinal submucosa (SIS-ECM) have been shown to promote the formation of site-specific tissue in a number of preclinical animal studies. (researchgate.net)
However, efficient harvest and handling of cell sheets remain challenging, including insufficient extracellular matrix content and poor mechanical strength. (researchgate.net)
Mechanical loading has been widely used to enhance extracellular matrix production in a variety of cell types. (researchgate.net)
We found that the extracellular matrix content and thickness of cell sheet were markedly elevated upon appropriate mechanical conditioning. (researchgate.net)
Unlike many other kinases, GSK3B is believed to be permanently active in resting cells and in neurons without extracellular stimulation and can be inactivated by Ser9 phosphorylation [ 14 ]. (biomedcentral.com)
More research teams are accelerating the use of other types of adult stem cells, in particular neural stem cells for diseases where beneficial outcome could result from either in-lineage cell replacement or extracellular factors. (biomedcentral.com)
These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
Our results proposed 16-h perfusion of 0.25% sodium dodecyl sulfate (SDS) + 0.5% Triton X-100 combination to the vessel as an optimal decellularization protocol in terms of cell elimination as well as extracellular matrix preservation. (biomedcentral.com)

Intradermal adipocytes form dermal white adipose tissue (dWAT), a unique fat depot localized in the lower layer of the dermis. (bvsalud.org)
Intradermal adipocyte progenitor cells are activated toward the end of the telogen phase to proliferate and differentiate into mature adipocytes. (jcadonline.com)
26,27 Dermal adipocytes are organized in small clusters under the reticular dermis and interact with the hair follicular cells regulating the hair cycle. (jcadonline.com)

Dermal macrophages are macrophages in the skin that facilitate skin homeostasis by mediating wound repair, hair growth, and salt balance. (wikipedia.org)
Dermal macrophages' ability to acquire pro-inflammatory properties also potentiates them in cancer defence. (wikipedia.org)
Dermal macrophages are native immune cells mainly located in the dermis of the skin. (wikipedia.org)
Dermal macrophages belong to the mononuclear phagocyte system that serves a vital role in the innate immunity of the skin. (wikipedia.org)
They are also a type of antigen-presenting cells (APCs) that can mediate the infiltration of immune cells during an immune response, suggesting dermal macrophages' influence on both the innate and adaptive immune systems. (wikipedia.org)
Dermal macrophages are either from embryonic progenitors or circulating progenitors. (wikipedia.org)
Numerous dermal macrophages are present in the skin at birth due to the infiltration of yolk-sac derived macrophages and haematopoietic stem cell (HSC)-derived monocytes. (wikipedia.org)
The prenatal population of dermal macrophages is gradually replaced by the recruitment and differentiation of circulating progenitors in adulthood. (wikipedia.org)
Prenatal dermal macrophages are not to be confused with Langerhans cells as they uniquely express CD14 and FXIIIa and lack CD1a. (wikipedia.org)
The prenatal portion of tissue-resident dermal macrophages is produced from yolk-sac derived precursors. (wikipedia.org)
The generation of dermal macrophages results from primitive haematopoiesis or definitive haematopoiesis. (wikipedia.org)
Primitive haematopoiesis allows the generation of yolk-sac derived macrophages and subsequent release into the foetal bloodstream for tissue infiltration and colonisation. (wikipedia.org)
In this process, cytokines like CSF1 are essential in facilitating the differentiation of monocytes into tissue-resident dermal macrophages and their survival. (wikipedia.org)
Therefore, the differentiation of monocytes to dermal macrophages is CSF-1/CSF1R dependent. (wikipedia.org)
The level of dermal macrophages from prenatal development remains detectible through constant, slow proliferation. (wikipedia.org)
The postnatal population of dermal macrophages is achieved by the infiltration of circulating monocytes given proper CCR2 signalling, a pathway responding to chemokines. (wikipedia.org)
The infiltration of bone-marrow-derived monocytes generated postnatally creates a distinct population of dermal macrophages. (wikipedia.org)
The generation of dermal macrophages is highlighted by the expression of cell markers such as CD64, MERTK and the downregulation of CCR2. (wikipedia.org)
Dermal macrophages can phagocytose and digest foreign substances similar to other cell types in the mononuclear phagocyte system. (wikipedia.org)
Dermal macrophages have a distinct expression of genes to facilitate their specialisation in removing macromolecules and foreign pathogens. (wikipedia.org)
Dermal macrophages' functions suggest their importance in the skin's innate immunity. (wikipedia.org)
Dermal macrophages, Langerhans cells and dendritic cells are the main types of antigen-presenting cells (APCs) in the skin. (wikipedia.org)
The latter refers to a special kind of inflamma tion that's characterised by the presence of activated macrophages (epithelioid cells) [url=https://archives.gov.pk/rx-pharmacy/order-dapsone-online-no-rx/] skin care 101 tips dapsone 100mg cheap mastercard[/url]. (ehd.org)
Most tissues harbor resident mononuclear phagocytes, that is, dendritic cells and macrophages. (hindawi.com)
Mononuclear phagocytes are a group of phenotypic distinct members, often referred to as either macrophages or dendritic cells (DC), that derive from myeloid precursors and that contribute to the functions of peripheral tissues [ 1 ]. (hindawi.com)
In this paper we apply the tissue-centric perspective to discuss the role of resident and infiltrating macrophages and dendritic cells in different organs. (hindawi.com)
All solid organs and most other tissues harbor a network of DC or macrophages (Table 1 ). (hindawi.com)

They can acquire an M1 or M2 phenotype to promote or suppress an inflammatory response, thereby influencing other cells' activity via the production of pro-inflammatory or anti-inflammatory cytokines. (wikipedia.org)
A more tissue-centric view of these processes, claiming that the tissues define phenotype and function of resident and infiltrating immune cells to meet tissues needs during homeostasis and disease, seems provocative [ 4 , 5 ]. (hindawi.com)
The AD-iPSCs were differentiated into neuronal cells, in order to generate disease-specific protein association networks modeling the molecular pathology on the transcriptome level of AD, to analyse the reflection of the disease phenotype in gene expression in AD-iPS neuronal cells, in particular in the ubiquitin-proteasome system (UPS), and to address expression of typical AD proteins. (biomedcentral.com)
Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells. (thermofisher.com)
It is grown in DMEM/F12 medium as standard, but its phenotype is dependent on culture conditions, and many differentiation markers are usually absent. (allergy-link.com)

Embryonic stem cells (ESC) can be expanded indefinitely without undergoing replicative senescence or aging due to their high telomerase expression. (biomedcentral.com)
An immunologically identical cardiac tissue can be produced by direct reprogramming from the patients' tissue without the potential risk of tumorigenesis that occurs with induced pluripotent stem cells [ 2 ] and the ethical issue using embryonic stem cells. (thno.org)
the EST, which assesses the effects of compounds on the differentiation of mouse embryonic stem cells into contracting cardiomyocytes, can be used to rank the potency of chemicals within a series of alkoxyacetic acid metabolites formed from The implementation of the European REACH (Registration, glycol ethers. (cdc.gov)

As greater understanding of the growth factors involved in wound healing emerges, future patient care may include scarless wound healing and transplant of tissues engineered from stem cell progenitors. (medscape.com)

They also can inspire angiogenesis by direct differentiation, cell-to-cell interaction, or paracrine effects. (biomedcentral.com)
Department of Cell and Structural Biology, University of Manchester M I3 9PL, U.K. t Department of ,tmmunology, University of Liverpool, P.O. Box 147, L69 3BX, U.K. Abstraet--Angiogenesis is an essential component of wound healing. (docksci.com)
Wound healing cannot occur without angiogenesis, The vasculature comprises up to 60% of repair tissue (Dyson et aI. (docksci.com)

A classification that sufficiently covers their phenotypic heterogeneity and plasticity during homeostasis and disease does not yet exist because cell culture-based phenotypes often do not match those found in vivo . (hindawi.com)
In this Chapter, we dissect the proof-of-principle studies that demonstrated that cellular reprogramming to pluripotency can be induced in vivo, in spite of unfavorable pro-differentiation signals present within the tissues. (springer.com)
The links of in vivo reprogramming to pluripotency with tumorigenesis and teratoma formation, and the cross-talk with cellular senesce and tissue injury are also discussed. (springer.com)
One of the kinases that phosphorylates tau in-vivo is glycogen synthase kinase-3β (GSK3B), which is widely expressed in all tissues with elevated expression in developing brains [ 13 ]. (biomedcentral.com)

Astroglia are highly heterogeneous neural cells primarily responsible for homeostasis and neuroprotection in the CNS. (nature.com)
Thus, the secretion of gall wasps stimulates plant cell growth and overrules homeostasis of the affected leaf area - this is similar to tumour outgrowth. (nature.com)
Advances in tissue engineering technology have led to the production of novel human skin equivalents and organoids that reproduce cell-cell interactions with tissue-scale tensional homeostasis, and enable us to evaluate skin tissue morphology, functionality, drug response and wound healing. (mdpi.com)
A tissue-centric view assumes that changing tissue microenvironments shape the phenotypes of their resident and infiltrating mononuclear phagocytes to fulfill the tissue's need to maintain or regain homeostasis. (hindawi.com)
Thus, organ- and disease phase-specific microenvironments determine macrophage and dendritic cell heterogeneity in a temporal and spatial manner, which assures their support to maintain and regain homeostasis in whatever condition. (hindawi.com)
We examine tissue needs to maintain homeostasis and how to regain homeostasis upon tissue injury. (hindawi.com)
These cells provide several important physiological functions during homeostasis (Figure 1 ). (hindawi.com)
In this context, regulatory T cells (Tregs) hold a key role in balancing immune homeostasis and mediating cutaneous wound healing. (biomedcentral.com)
Diseases associated with defects in adipocyte homeostasis, such as lipodystrophy and focal dermal hypoplasia, lead to alopecia. (jcadonline.com)

Dulbecco's Phosphate Buffered Saline (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. (sigmaaldrich.com)
DPBS buffer is used to irrigate, wash, and dilute mammalian cells while maintaining osmotic balance. (sigmaaldrich.com)
Viable offspring derived from fetal and adult mammalian cells. (springer.com)

These epidermal and connective tissue layers protect against mechanical and chemical harm and repel pathogenic invaders [ 19 ]. (biomedcentral.com)
Adipose tissue is a loose connective tissue composed of cells supported by an intracellular matrix as well as by vascular, lymphatic, and neural networks. (jcadonline.com)
These lesions often replace the dermal papillae, but they can also extend into the deeper connective tissue. (medscape.com)

As summarized below and detailed in Chapter 3, health effects that have been associated with exposure to PCBs in humans and/or animals include liver, thyroid, dermal and ocular changes, immunological alterations, neurodevelop- mental changes, reduced birth weight, reproductive toxicity, and cancer. (cdc.gov)
Animal studies have shown that PCBs induce effects in monkeys at lower doses than in other species, and that immunological, dermal/ocular, and neurobehavioral changes are particularly sensitive indicators of toxicity in monkeys exposed either as adults, or during pre- or postnatal periods. (cdc.gov)

Treatment for this condition remains difficult and largely ineffective, because the growth of cells in the wound is inhibited ( 2 ) and chemotherapy usually leads to myelosuppression, the latter of which further compromises the migration of multipotent cells from the bone marrow ( 3 ). (ijbs.com)

Also, following transplantation and migration to target tissue, they encounter a harsh milieu accompanied by death signals because of the lack of proper tensegrity structure between the cells and matrix. (biomedcentral.com)
Hu G, Liu P, Feng J, Jin Y. Transplantation with Bone Marrow Stromal Cells Promotes Wound Healing Under Chemotherapy through Altering Phenotypes. (ijbs.com)
Stem cell transplantation is a promising strategy for delayed wound healing caused by chemotherapy. (ijbs.com)
Nevertheless, myelosuppression can be reversed by autologous stem cell transplantation (SCT), even without additional toxicity ( 4 , 5 ). (ijbs.com)
Transplantation of living nuclei from blastula cells into enucleated frogs' eggs. (springer.com)

The timeline for cell migration in a normal wound healing process is predictable. (medscape.com)
Integrin-based effects on growth factor re-ceptors include enhancement of cell migration (11, 12), survival (13), and proliferation (14 -17). (123dok.org)

10. Dumevska B, Chami O, McKernan R, Divya Goel Uli Schmidt (2016) Derivation of Genea052 human embryonic stem cell line. (yoproteins.eu)
16 Although induced pluripotent stem cell (iPSC) technology can be used to investigate human astrocyte development and function, only a limited number of studies have described homogeneous generation of healthy astrocytes. (nature.com)
In this review, we analyze the therapeutic agents that have been used for AGA and emphasize the potential of new therapies based on advances in stem cell technologies and regenerative medicine. (benitonovas.com)
Stem cell therapy has been the subject of many studies for its potential to cure many disorders. (biomedcentral.com)
Embryonic stem cell lines derived from human blastocysts. (springer.com)
Evans MD, Kelley J. US attitudes toward human embryonic stem cell research. (springer.com)
Recent advances in stem cell therapy for tissue engineering approaches have provided promising data on wound repair and tissue regeneration. (biomedcentral.com)
The rapid advance of stem cell clinical trials for a broad spectrum of conditions warrants an update of the review by Trounson (2009) [ 1 ]. (biomedcentral.com)
There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)
Mora MV, Ibán MAR, Heredia JD, Laakso RB, Cuéllar R, Arranz MG. Stem cell therapy in the management of shoulder rotator cuff disorders. (wjgnet.com)
physiologically based animals because a mouse embryonic stem cell line is used. (cdc.gov)

It has been found that some of the SVF cell types possess regenerative and anti-inflammatory potentials in damaged tissues due to their ability to secrete growth factors and anti-inflammatory molecules. (jcadonline.com)

11 , 12 With the emergence of stem cells (SCs), many mechanisms that lead to tissue regeneration have been discovered. (benitonovas.com)
Pre-clinical studies are however necessary to validate the best skin regeneration technique, which could be used in pediatric surgical translational research. (biomedcentral.com)

To gain pluripotency, the reprogramming process typically involves transfecting adult somatic cells with certain pluripotency markers. (biomedcentral.com)
The developmental capacity of nuclei transplanted from keratinized skin cells of adult frogs. (springer.com)
The differences between fetal and adult scars suggest the possibility of manipulating skin scarring outcomes. (rbcp.org.br)
This study aimed to assess whether the use of adult stem cells from adipose tissue is beneficial to skin healing. (rbcp.org.br)
The adult stem cells used were autologous and were extracted from infraumbilical adipose tissue prior to abdominoplasty. (rbcp.org.br)
Skin healing results after implantation of adult stem cells derived from adipose tissue were satisfactory. (rbcp.org.br)

After a short period, capillary vasodilatation occurs secondary to local histamine release, and the cells of inflammation are able to migrate to the wound bed. (medscape.com)

However, their antigen-presenting ability is relatively lower than dendritic cells (DCs) and Langerhans cells (LCs) in the skin. (wikipedia.org)

Apoptosis was measured by Hoechst 33258/propidium iodide double staining of nuclear chromatin and the formation of gaps into the lymphendothelial barrier in a three-dimensional co-culture model consisting of MCF-7 tumour cell spheroids and human lymphendothelial monolayers. (nature.com)
In HL-60 leukaemia cells, di-GA activated caspase 3 and dose-dependently induced apoptosis. (nature.com)
These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)
Although most cells have vitamin D receptors, several key genes that encode proteins and peptides are modulated in part by vitamin D and thus affect musculoskeletal functions, mitochondrial respiration, cell growth, proliferation, differentiation, and apoptosis [13]. (vitamindwiki.com)

After implantation into the critical-sized calvarial defects of mice, the mechanically conditioned cell sheets significantly promoted new bone formation. (researchgate.net)
Findings from this study reveal that thermo-responsive elastomer, together with mechanical conditioning, can potentially be applied to prepare high-quality cell sheets for bone tissue engineering. (researchgate.net)
Herein we characterized human fetal bone marrow stromal cells (hBMSCs) during wound healing in mice treated with cyclophosphamide (CTX). (ijbs.com)
A significant proportion of clinical studies that are underway involve bone marrow and cord blood stem cells for blood and immune disorders [ 3 ] and cancers. (biomedcentral.com)
Despite improved surgical techniques, the tendon-to-bone healing rate is unsatisfactory due to difficulties in restoring the delicate transitional tissue between bone and tendon. (wjgnet.com)
The epidermis is derived primarily from surface ectoderm but is colonized by pigment-containing melanocytes of neural crest origin, antigen-processing Langerhans cells of bone marrow origin, and pressure-sensing Merkel cells of neural crest origin. (medscape.com)

Mononuclear phagocytes contributions to tissue pathologies relate to their central roles in orchestrating all stages of host defense and wound healing, which often become maladaptive processes, especially in sterile and/or diffuse tissue injuries. (hindawi.com)
Understanding how the body repairs damaged tissue and what factors influence the wound healing process helps the surgeon ensure an acceptable outcome from surgery. (medscape.com)
Wound healing in any tissue follows a predictable sequence of events. (medscape.com)
Although seemingly basic in concept, advances in molecular science have allowed modern medicine to gain a true appreciation of the complex interplay between the cells involved in the phases of wound healing. (medscape.com)
After injury to tissue occurs, the cell membranes, damaged from the wound formation, release thromboxane A2 and prostaglandin 2-alpha, potent vasoconstrictors. (medscape.com)
Cells involved in wound healing. (medscape.com)
The cells appearing in a wound are depicted in sequence from left to right, and the color bars represent the range of days each cell type is in the wound. (medscape.com)
These cells were implanted into the surgical wound dermis in the suprapubic region before skin synthesis. (rbcp.org.br)
The subsequent wound healing processes aim to restore dermal and epidermal integrity and functionality. (biomedcentral.com)
Specifically, the timely orchestration of pro- and anti-inflammatory responses, based on the activation and inhibition of various cell types, such as tissue-resident cells and different types of immune cells, can significantly impact wound repair [ 7 ]. (biomedcentral.com)
While researchers agree on the abundance of Tregs in distinct dermal layers, the exact involvement of Tregs in cutaneous wound healing remains to be elucidated [ 18 ]. (biomedcentral.com)
Wound healing is a complex physiological process necessitating the coordinated action of various cell types, signals and microRNAs (miRNAs). (molcells.org)

95% homogeneous populations of human astrocytes within 30 days of differentiation from cortical neural progenitor cells (NPCs). (nature.com)

In turn, the plant produces GA, which seems to combat the improper growth signals and re-establishes cell-cycle control. (nature.com)
15 , 16 Some of these signals stimulate differentiation of SCs to keratinocytes which are important for HF growth. (benitonovas.com)
17 Other signals can stimulate dermal papilla cells (DPCs) that promote SC proliferation in the HF. (benitonovas.com)
19 - 21 Other signals, such as those from endothelial cells (ECs) located at the DP, are also essential for HF maintenance. (benitonovas.com)
In the heart, cardiomyocytes interact with neighboring cells through direct cell-cell interactions, various secreted cytokines, and electric signals (Figure 1 A) [ 24 ]. (thno.org)

The mildest form is spina bifida aperta, in which osseous fusion of one or more vertebral arches is lacking, without involvement of the underlying meninges or neural tissue. (medscape.com)
Pannexin 1 (PANX1) is expressed in many tissue types including tissues of neural origin. (jcancer.org)
It is widely accepted that the cell origin for NB arises from the sympathoadrenal lineage of the neural crest during development [ 3 ]. (jcancer.org)
The neural crest is present during embryogenesis and gives rise to diverse cell types including enteric neurons and glia, as well as peripheral neurons [ 3 ]. (jcancer.org)
Panx1 in rodents), which forms homo-heptameric single membrane channels permeable to ion and metabolite [ 7 , 8 ], is expressed in many tissue types including tissues of neural origin [ 9 ]. (jcancer.org)
Melanocytes, derived from neural crest cells, primarily function to produce a pigment, melanin, which absorbs radiant energy from the sun and protects the skin from the harmful effects of UV radiation. (medscape.com)

Biologic factors that include signaling from stem cells, dermal papilla cells, and platelet-rich plasma are some of the current therapeutic agents being studied for hair restoration with milder side effects. (benitonovas.com)

This slightly different perspective may somewhat shape our understanding of macrophage heterogeneity and tissue pathology but certainly also raise new questions for future research. (hindawi.com)
Due to their considerable plasticity and heterogeneity, the tissue-based DC and macrophage populations have been defined as mononuclear phagocytes [ 1 , 6 , 7 ]. (hindawi.com)

The mammalian skin consists of three main layers: the epidermis, the dermis, and the subcutaneous tissue, including the fascia (Fig. 1 ). (biomedcentral.com)
Adipose cells organized in small clusters under the reticular dermis closely interact with hair follicular cells and regulate the hair cycle. (jcadonline.com)
Denatured dermis caused by deep skin burns is strongly associated with functional impairment, cell metabolism disorders and pathologically morphological changes. (molcells.org)

Transcriptome analysis of AD-iPS derived neuronal cells revealed significant changes in the expression of genes associated with AD and with the constitutive as well as the inducible subunits of the proteasome complex. (biomedcentral.com)

showed that the differentiation assay of benchmark dose. (cdc.gov)

They construct the mononuclear phagocyte system together with dendritic and Langerhans cells. (wikipedia.org)

Adipose-derived stem cells (ASCs) from human and animal fat have emerged as therapeutic alternatives for damaged tissues. (bvsalud.org)

To achieve this we firstly established a mouse BAT enriched-miRNA profile by comparing miRNAs expressed in mouse BAT, white adipose tissue and skeletal muscle. (biomedcentral.com)

Nano-thin and nano-porous membranes and heart like electric stimulus were used in the cardiac-mimetic cell-culture system. (thno.org)

The immunohistochemical (IHC) staining of CD31, α-SMA, and vimentin markers suggested placement of seeded cells in the suitable sites and considerable elevation of cell attachment within the scaffolds bioconjugated with apelin-13 compared to the non-bioconjugated, and decellularized groups. (biomedcentral.com)
Pyruvate, in combination with DMEM, induced dark pigmentation and promoted differentiation markers such as CRALBP and MerTK. (allergy-link.com)
This study demonstrated important differentiation markers, including pigmentation and Western blots of RPE65 protein, and showed human POS phagocytosis in ARPE-19 cultures using a simple differentiation protocol. (allergy-link.com)
Introduction The research of Hatch (1) has greatly increased our understand- ing of the use and potential limitations of biological markers for adverse reproductive effects. (cdc.gov)
This area of research has lagged con- siderably behind the field of developing new effect markers, such as semen analysis and early pregnancy loss. (cdc.gov)
Exposure to a potential fetal health hazard can be estimated through ecological assessment (e.g., testing the community water supply), questionnaires (e.g., classifying residents accord- ing to whether they drink bottled or tap water), or biological markers (e.g., testing for exposures to chemicals or solvents found in tap water). (cdc.gov)

To this end, multiple tissue-resident cells and recruited immune cells cooperate to efficiently repair the injured tissue. (biomedcentral.com)
This function of the cutaneous system is mediated by a myriad of highly specialized immune cells. (biomedcentral.com)
In interaction with other non-immune cells, such immune cell subsets ensure the functionality and integrity of the skin layers [ 1 ]. (biomedcentral.com)

Superficial lesions consist of dilated lymph vessels lined by flat endothelial cells in a discontinuous layer immediately subjacent to the oral epithelium. (medscape.com)
Negative WT1 staining implies a nonproliferative nature of the oral lymphangiomas, thus growth is most likely secondary to enlargement of lymphatic vessels and not neoplastic proliferation of lymphatic endothelial cells. (medscape.com)

Recently, the production and secretion of growth factors has been identified as an essential function of adipose-derived stem cells (ADSCs). (indexindex.com)

To this end and within their broad functionality and operationality, Tregs are able to adopt unique features of skin cells and contribute to the skin's role as an immune barrier. (biomedcentral.com)

The main property that sets ESC apart from other cells is their pluripotent nature, meaning they can give rise/differentiate to cells of the three primary germ layers (mesoderm, endoderm and ectoderm) [ 13 ]. (biomedcentral.com)
During the fourth week of embryologic development, the single cell thick ectoderm and underlying mesoderm begin to proliferate and differentiate. (medscape.com)

Understanding the mechanisms controling BAT differentiation may identify potential therapeutic targets to enhance BAT-controlled metabolism and to increase EE. (biomedcentral.com)

The epidermis is a stratified, squamous epithelium that consists primarily of keratinocytes in progressive stages of differentiation from deeper to more superficial layers. (medscape.com)
This single cell layer of keratinocytes is attached to the basement membrane via hemidesmosomes. (medscape.com)

ARPE-19 cells maintained in this medium could also phagocytose human photoreceptor outer segments (POS). (allergy-link.com)

In this review, we discuss the potential influence of stromal vascular fraction from adipose tissue in the management of alopecia as well as its involvement in preclinical and clinical trials. (jcadonline.com)
however, a variety of other cell types are found as well, collectively labeled as stromal vascular fraction (SVF). (jcadonline.com)

CD326 mediates calcium-independent, homophilic, cell-cell adhesion and may function as a growth factor receptor. (thermofisher.com)
In conclusion, our results provide a platform in which xenograft vessels are decellularized properly in a short time, and the recellularization process is significantly improved after the bioconjugation of the acellular scaffold with apelin-13 in terms of cell adhesion and viability within the scaffold. (biomedcentral.com)

Stem cells and regenerative medicine - future perspectives. (springer.com)
Stem cells in regenerative medicine: introduction. (springer.com)

1991), and the original name for the temporary organ of repair, 'granulation tissue' (Hunter, 1787), is derived from the prominence of its vessels. (docksci.com)

The pathways involved in cellular differentiation or transformation are Smad, Rho proteins, and PI3-kinase. (molvis.org)

22 The underlying mechanism is believed to be a higher concentration of dihydrotestosterone (DHT) to androgen-sensitive hair follicles, causing thinning of the dermal papillae and shortening of the anagen phase. (jcadonline.com)

Exposure to PCBs has been assessed by measuring PCBs in blood, breast milk, and adipose tissue. (cdc.gov)
Nevertheless, over exposure to sunlight, does not cause hypervitaminosis D but can cause damage to skin cells. (vitamindwiki.com)
It serves as a protective barrier that prevents internal tissues from exposure to trauma, ultraviolet (UV) radiation, temperature extremes, toxins, and bacteria. (medscape.com)

However, the presence of excess numbers of myofibroblasts in wounded tissue is undesirable because of the risk of fibrotic scar formation. (molvis.org)
These cells were subsequently transferred to Vitrogen-coated Transwell inserts (0.5 ml of diluted Vitrogen per insert, see next section for more details), and the inserts were suspended above a confluent feeder layer of cultured human gallbladder myofibroblasts. (pancreapedia.org)
Cultured human gallbladder myofibroblasts (HGBMF) are used as a feeder layer, providing factors (yet uncharacterized) that support the growth and differentiation of the dog PDEC. (pancreapedia.org)

Monocytes are produced from haematopoietic stem cells in the liver, and they subsequently infiltrate the skin via the foetal bloodstream. (wikipedia.org)

Interestingly, bundles of aligned and uniformly spaced collagen fibrils were more prevalent in keratocytes in Y-27632-treated corneas, which is reminiscent of fibripositor-like structures that have been proposed as a mechanism of matrix deposition in embryonic connective tissues. (molvis.org)
In some antiphospholipid syndrome lesions, hemosiderin deposition can make differentiation from Kaposi sarcoma difficult. (medscape.com)

The effect of PNIPAAm grafting yields on cell behaviours was investigated to optimize surfaces suitable for cell sheet culturing and harvesting. (researchgate.net)
27 Together, dermal blood vessels and DPCs orchestrate a suitable microenvironment for the growth and survival of HFSCs. (benitonovas.com)
Tissue engineering of native vessels is an alternative approach for patients with vascular disease who lack sufficient saphenous vein or other suitable conduits for autologous vascular graft. (biomedcentral.com)

growth, differentiation, and survival. (123dok.org)

Plating human gallbladder myofibroblast cells (HGBMF). (pancreapedia.org)
Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3^+ cells. (thermofisher.com)
Intracellular staining of human peripheral blood mononuclear cells (PBMCs) with PCH101 antibody using the anti-human Foxp3 Staining Set and protocol reveals approximately 0.5-4% of lymphocytes staining, with the majority of staining occurring in the CD25^bright population. (thermofisher.com)
Induction of differentiation by pyruvate and DMEM in the human retinal pigment epithelium cell line ARPE-19. (allergy-link.com)
The human RPE cell-line ARPE-19 is used widely as an alternative to primary RPE. (allergy-link.com)
Discovery of active human brown adipose tissue (BAT) has renewed interest in the possibility that it may regulate metabolism and EE in humans [ 3 - 5 ]. (biomedcentral.com)

Anatomy61

Organisms11

Diseases13

Chemicals and Drugs49

Analytical, Diagnostic and Therapeutic Techniques and Equipment33

Psychiatry and Psychology3

Phenomena and Processes54

Disciplines and Occupations16

Anthropology, Education, Sociology and Social Phenomena6

Humanities9

Information Science3

Named Groups3

Health Care17

Geographicals1

Fibroblasts15

Mesenchymal15

Vitro10

Serum5

Fibroblast2

Epithelial10

MSCs10

Proliferation and differentiation1

Extracellular9

Adipocytes3

Macrophages27

Phenotype5

Embryonic stem3

Progenitors1

Angiogenesis3

Vivo4

Homeostasis9

Mammalian cells3

Connective tissue3

Immunological2

Multipotent cells1

Transplantation5

Migration2

Stem cell11

Potentials1

Regeneration2

Adult6

Inflammation1

Dendritic cells1

Apoptosis4

Bone6

Wound12

Progenitor cells1

Signals5

Neural6

Signaling from stem1

Macrophage2

Dermis3

Genes1

Assay1

Langerhans1

ASCs1

White adipos1

Membranes1

Markers6

Immune cells3

Endothelial cells2

Adipose-derived stem cells1

Skin's1

Differentiate2

Therapeutic1

Keratinocytes2

Phagocytose1

Stromal vascular fraction2

Adhesion2

Regenerative medicine2

Granulation tissue1

Pathways1

Papillae1

Exposure3

Myofibroblasts3

Liver1

Deposition2

Suitable3

Survival1

Human6