Cells of catharanthus roseus. Medical search. Frequent questions

In the 1950s, vinca alkaloids, including vinblastine and vincristine, were isolated from Catharanthus roseus while screening for anti-diabetic drugs. (wikipedia.org)
The research team studied the biosynthesis of two alkaloids from the plant Catharanthus roseus that are used in human medicine as anti-cancer agents. (analytica-world.com)
Many plant natural products are highly complex molecules, such as the alkaloids vincristine and vinblastine, which are produced by the Madagascar periwinkle Catharanthus roseus . (analytica-world.com)
The chemo drugs vincristine and vinblastine, vinca alkaloids, although they come from a different plant, Catharanthus roseus, aka the Madagascan Periwinkle, work in the same manner - inhibiting cell division. (acsh.org)
5. Alam MM, Naeem M, Khan MMA and Uddin M. Vincristine and vinblastine anticancer catharanthus alkaloids: pharmacological applications and strategies for yield improvement. (jmp.ir)
9. Hedhili S, Courdavault V, Giglioli-Guivarc'h N, Gantet P. Regulation of the terpene moiety biosynthesis of Catharanthus roseus terpene indole alkaloids. (jmp.ir)
Led by Chenxin Li of UGA's Center for Applied Genetic Technologies in the College of Agricultural and Environmental Sciences, the research team studied the biosynthesis of two alkaloids from the Madagascar periwinkle (Catharanthus roseus) that are used as anti-cancer agents in human medicine. (hortidaily.com)
The research team is confident that the organization of biosynthetic pathways for medicinally relevant alkaloids in Catharanthus roseus is not an isolated phenomenon. (hortidaily.com)
In the new study, they engineered the cells to express genes that code for enzymes that attach chlorine or bromine to vinblastine precursors and other alkaloids. (mit.edu)
The inhibitory effect of E. brevicornum alkaloids on human cervical cancer HeLa cells was investigated. (chinbullbotany.com)
E. brevicornum alkaloids exhibited a significant dose-dependent inhibitory activity against human cervical cancer HeLa cells. (chinbullbotany.com)
The invaluable antineoplastic bisindole alkaloids of Catharanthus roseus and their precursor, vindoline, are not produced in cell cultures. (braf-signal.com)
cultures caused transcriptional upregulation of SGD and enhanced the accumulation of total alkaloids but did not produce vindoline as DAT transcripts were always found to be absent in suspension-cultured cells. (braf-signal.com)
The alkaloids vinblastine and vincristine, which do not have an antidiabetic effect but act on murine leukaemia cells, were soon isolated. (martifarm.com)
One way in which cells have developed resistance against the vinca alkaloids is by drug efflux. (smpdb.ca)
The anticancer agents vinblastine and vincristine are bisindole alkaloids derived from coupling vindoline and catharanthine, monoterpenoid indole alkaloids produced exclusively by the Madagascar periwinkle (Catharanthus roseus). (uea.ac.uk)

To analyze the morphological and aesthetic responses of zinnia ( Zinnia elegans L.) and periwinkle ( Catharanthus roseus (L.) G. Don) to different salinity stress levels, the following treatments were performed: s0 = municipal water (control), s1 = 3 dS m −1 , s2 = 4.5 dS m −1 , and s3 = 6 dS m −1 . (mdpi.com)
Left top) Catharanthus roseus, aka the Madagascan Periwinkle, a member of the Vinca plant family, is the source of the cancer drug vincristine (Right top) Nice structure, huh? (acsh.org)
Review of abiotic and biotic elicitors' roles in secondary metabolites biosynthesis of periwinkle (Catharanthus roseus (Linn. (jmp.ir)
Amiri S, Fotovat R, Panahi B, Tarinezhad A, Mohammadi S A. Review of abiotic and biotic elicitors' roles in secondary metabolites biosynthesis of periwinkle ( Catharanthus roseus (Linn. (jmp.ir)
6. Amiri S, Fotovat R, Tarinejad A, Panahi B, Mohammadi S.. In vitro regeneration of periwinkle (Catharanthus roseus L.) and fidelity analysis of regenerated plants with ISSR Markers. (jmp.ir)
MIT associate professor Sarah O'Connor, right, and graduate student Weerawat Runguphan have engineered periwinkle root cells that can produce variations of compounds that the plant normally synthesizes. (mit.edu)
In previous experiments, O'Connor and her students induced periwinkle root cells to create novel compounds by feeding them slightly altered versions of their usual starting materials. (mit.edu)
And at the start of that road is a beautiful flower called Catharanthus roseus, commonly known as the Madagascar periwinkle, which set the course for decades of exploration. (brocku.ca)
One of the plants they studied was the Madagascar periwinkle (L. Catharanthus roseus), which has traditionally been used to treat diabetes. (martifarm.com)
Vincristine (also named leurocristine) is a natural alkaloid isolated from the leaves of the Catharanthus roseus (commonly known as the Madagascar periwinkle). (smpdb.ca)
A potent inhibitor of cell division and used against lymphomas and testicular, breast, bladder and lung cancers, it is found in the leaves of Madagascar periwinkle ( Catharanthus roseus ). (healthproblemsnews.com)

In its natural rage along the dry coasts of southern Madagascar, Catharanthus roseus is considered weedy and invasive, often self-seeding prolifically in disturbed areas along roadsides and in fallow fields. (wikipedia.org)

Notable TIAs include vincristine and vinblastine of Catharanthus roseus that have anti-tumor activity. (biomedcentral.com)
The mechanism of vincristine is the inhibition of microtubule dynamics that would cause mitotic arrest and eventual cell death. (smpdb.ca)
Industrial production of vinblastine and vincristine currently relies on isolation from C. roseus leaves, a process that affords these compounds in 0.0003-0.01% yields. (uea.ac.uk)

By using single-cell analyses, the scientists were able to discover new genes important for biosynthesis and show that the intermediates of the metabolic pathway accumulate in specific cell types. (analytica-world.com)
Therefore, the goal of the current study was to use a new set of methods collectively termed single-cell omics to investigate specialized and rare cell types that play a central role in the biosynthesis of plant natural products, and whose signals are often obscured by more abundant cell types in plant organs. (analytica-world.com)
The abundant other cells in the leaf dilute the high concentration when whole leaves are crushed," said Sarah O'Connor, head of the Department of Natural Product Biosynthesis at MPI. (hortidaily.com)
Our new spotlight published in Trends in Plant Science, on a report by Li et al on single-cell multi-omics approach used to elucidate the architecture and regulation of anticancer alkaloid biosynthesis in medicinal plants. (univ-tours.fr)
Members of the selection committee and the executive were very impressed with your outstanding contributions to the field of plant biochemistry, notably towards understanding the metabolic pathways involved in the biosynthesis of the anticancer drug vinblastine in the medicinal plant Catharanthus roseus ," CSPB President Daphne Goring wrote to De Luca earlier this year when informing him of his award. (brocku.ca)
However, due to the leaf-specific localization of vindoline biosynthesis, and the lack of production of this precursor in cell suspension and hairy root cultures of C. roseus, further elucidation of this pathway demands the development of reverse genetics approaches to assay gene function in planta. (uea.ac.uk)

The first stage is expressed exclusively in specialized cells associated with vascular bundles in the leaf, called IPAP. (analytica-world.com)
The second stage of the biosynthetic pathway is expressed only in cells of the epidermis, the layer of cells that cover the leaves, and the last known steps of the biosynthetic pathway are expressed exclusively in idioblasts, a rare cell type of the leaf," Chenxin Li summarizes the results. (analytica-world.com)
Read our new study with Pr Chao SUN's group, on single-cell RNA sequencing of Catharanthus roseus leaf published in Nature plants! (univ-tours.fr)
They are involved in regulating cell elongation and division, vascular differentiation, photomorphogenesis, leaf angle inclination, seed germination, stomata development, as well as suppression of leaf senescence and abscission. (5htreceptor.com)
2018 ). It was also confirmed that AgNPs synthesized by the Juniperus chinensis leaf showed the potent anticancer effect on the human adenocarcinoma gastric (AGS) cell lines (Al-Dhafri and Ching 2019 ). (springeropen.com)
Leaf extracts of Alstonia angustiloba, Calotropis gigantea, Catharanthus roseus, Nerium oleander, Plumeria obtusa , and Vallaris glabra displayed positive APF activities. (phcogres.com)

The newer semi-synthetic chemotherapeutic agent vinorelbine, used in the treatment of non-small-cell lung cancer, can be prepared either from vindoline and catharanthine or from the vinca alkaloid leurosine, in both cases via anhydrovinblastine. (wikipedia.org)
Two important precursors of vinblastine - catharanthine and vindoline - occur in the idioblast cells at millimolar concentrations, about three orders of magnitude higher than vinblastine itself. (hortidaily.com)
However, this observation makes sense in that catharanthine and vindoline were found only in the rare idioblast cells. (hortidaily.com)
The utility of this approach in understanding gene function in C. roseus leaves is demonstrated by silencing known vindoline biosynthetic genes previously characterized in vitro. (uea.ac.uk)

Flow cytometric analysis evidenced that piperine arrested the cell cycle at growth 2/mitosis phase, hence suppressed the breast cancer progression. (ijpsonline.com)
But what all these life forms have in common is that their genetic code is copied from cell to cell thanks to the process of mitosis, whereby the nucleus of a cell splits into two before the cell divides. (visionlearning.com)
The term mitosis refers specifically to the process whereby the nucleus of a eukaryotic cell splits into two identical daughter nuclei prior to cell division. (visionlearning.com)
The rate at which mitosis occurs depends on the cell type. (visionlearning.com)
Background: Catharanthus roseus could inhibit the mitosis in tumor cell, while Curcuma xanthorhiza beside could activate immune system it also more triggered Catharanthus roseus to killed cancer. (undip.ac.id)
Broadly, most chemotherapeutic drugs work by impairing mitosis ( cell division ), effectively targeting fast-dividing cells . (wikidoc.org)
The term chemotherapy has come to connote non-specific usage of intracellular poisons to inhibit mitosis (cell division) or induce DNA damage, which is why inhibition of DNA repair can augment chemotherapy. (worldsbest.rehab)
Traditional chemotherapeutic agents are cytotoxic by means of interfering with cell division (mitosis) but cancer cells vary widely in their susceptibility to these agents. (worldsbest.rehab)

Catharanthus roseus is a perennial species of flowering plant in the family Apocynaceae. (stuartxchange.org)
7. Chen Q, Lu X, Guo X, Guo Q and Li D. Metabolomics characterization of two apocynaceae plants, Catharanthus roseus and Vinca minor, using GC-MS and LC-MS methods in combination. (jmp.ir)

Analysis of the interface between primary and secondary metabolism in Catharanthus roseus cell cultures using 13C-stable isotope feeding and coupled mass spectrometry. (mpg.de)
In this study, laboratory scale biotechnical production processes were developed for different types of plant cell cultures: embryogenic cell culture, undifferentiated cell suspension culture and hairy root culture. (vtt.fi)
Radioactive tracer studies in cell cultures of Catharanthus roseus established the steps of the BR metabolic pathway. (5htreceptor.com)

As the analyses showed, the entire biosynthetic pathway for the alkaloid vinblastine is organized in three stages and three discrete cell types. (analytica-world.com)
It induced apoptosis, suppressed cell migration and invasion, blocked cell cycle and phosphatidylinositol 3-kinase/ protein kinase B signaling pathway. (ijpsonline.com)
As the analyses showed, the entire biosynthetic pathway for the alkaloid vinblastine is organized into three stages and three discrete cell types. (hortidaily.com)
The researchers measured the concentrations of several intermediates in the metabolic pathway for vinblastine in single cells and were surprised by the results. (hortidaily.com)
In addition, analysis of genes expressed simultaneously in a particular cell type has helped us identify new players in this metabolic pathway, said Robin Buell, the Georgia Research Alliance (GRA) Eminent Scholar Chair in Crop Genomics at UGA. (hortidaily.com)

angusta Steenis Catharanthus roseus is an evergreen subshrub or herbaceous plant growing 1 m (39 in) tall. (wikipedia.org)

Lochnera rosea f. alba (G.Don) Woodson Lochnera rosea var. (wikipedia.org)
Lochnera rosea var. (wikipedia.org)

In conclusion, the current investigation regarding anticancer effects of piperine against breast cancer revealed remarkable suppression of cancer cells. (ijpsonline.com)
The extracts of Vinca have demonstrated significant anticancer activity against numerous cell types 10 . (ijpsr.com)
2016 ). This study was conducted to analyze the anticancer effects of green-synthesized AgNPs using Juniperus polycarpos on A549 (adenocarcinomic human alveolar basal epithelial cells) cell lines, as well as comparing it with Cisplatin commercial drug. (springeropen.com)

They are widely distributed in plants from different families and their effect stems from the ability to interact with membrane sterols, disrupting the cell integrity from target organisms ( 34 - 37 ). (elifesciences.org)
A VIGS method was developed herein to investigate gene function in C. roseus plants using the pTRV vector system. (uea.ac.uk)

Factorial design was also tested for the optimization of alkaloid production medium for a cell suspension of Catharanthus roseus (L.) G. Don, but the conventional method of parallel testing of different media proved to be more successful. (vtt.fi)

The scientists know that gene activity is not the same in all cells of a plant and that the chemistry can differ drastically from cell to cell. (analytica-world.com)
Liscombe, DK & O'Connor, SE 2011, ' A virus-induced gene silencing approach to understanding alkaloid metabolism in Catharanthus roseus ', Phytochemistry , vol. 72, no. 16, pp. 1969-1977. (uea.ac.uk)

A rapid and sensitive bioassay for chitin oligosaccharides was also used employing suspension-cultured plant cells of Catharanthus roseus. (url.edu)

4. Dugede Bernonville T, Clastre M, Besseau S, Oudin A, Burlat V, Glévarec G, Lanoue A, Papon N, El-Sayed M. and Verpoorte R. Growth, metabolic profiling and enzymes activities of Catharanthus roseus seedlings treated with plant growth regulators. (jmp.ir)

The objective of this research is to find out the effect of Catharanthus roseus and Curcuma xanthorhiza in Tumor Size macroscopically and Lungs Histology Description and axilla lymph MiceC3H inoculated by Adenocarcinoma mammae. (undip.ac.id)
Conclusion: The providing of Catharanthus roseus 19.5 mg/object/day and Curcuma xanthorhiza 10.4 mg/object/day combination for three weeks resulted in the difference of weigh and volume of tumor. (undip.ac.id)
With succeeding generations of tumor cells, differentiation is typically lost, growth becomes less regulated, and tumors become less responsive to most chemotherapeutic agents. (wikidoc.org)
Because only a fraction of the cells in a tumor die with each treatment (fractional kill), repeated doses must be administered to continue to reduce the size of the tumor. (worldsbest.rehab)

The growth as well as the production characteristics and the nutrient uptake of the different plant cell systems were determined. (vtt.fi)
Cancer is the uncontrolled growth of cells coupled with malignant behavior: invasion and metastasis . (wikidoc.org)
This means that other fast dividing cells such as those responsible for hair growth and for replacement of the intestinal epithelium (lining) are also often affected. (wikidoc.org)
As chemotherapy affects cell division, tumors with high growth fractions (such as acute myelogenous leukemia and the aggressive lymphomas , including Hodgkin's disease ) are more sensitive to chemotherapy, as a larger proportion of the targeted cells are undergoing cell division at any time. (wikidoc.org)
Drugs affect "younger" tumors (i.e. more differentiated) more effectively, because mechanisms regulating cell growth are usually still preserved. (wikidoc.org)
Extracts of C. gigantea and V. glabra inhibited the growth of all six cancer cell lines while all extracts of N. oleander were effective against MCF-7 cells. (phcogres.com)

Against The Human Promyelocytic leukemia (HL-60) and The Human Cervical Cancer (HeLa) Cells. (unisza.edu.my)

8. Sain M and Sharma V. Catharanthus roseus (An anti-cancerous drug yielding plant). (jmp.ir)

Cell migration as well as cell invasive ability of MCF-7 cells was reduced to minimum by the application of piperine in dose dependent-manner. (ijpsonline.com)

The concentration of the two precursors in these cells was much higher than we expected and even exceeded their concentrations in whole organ extracts. (hortidaily.com)
The aim of the present study was to determine the antibacterial activity of crude extracts from root parts of Catharanthus roseus against several bacterial species of clinical significance. (ijpsr.com)
Dichloromethane (DCM) extract of C. gigantea, and DCM and DCM:MeOH extracts of V. glabra showed strong APF activities against all six human cancer cell lines. (phcogres.com)
Against breast cancer cells of MCF-7 and MDA-MB-231, DCM extracts of C. gigantea and N. oleander were stronger than or comparable to standard drugs of xanthorrhizol, curcumin, and tamoxifen. (phcogres.com)
All four extracts of N. oleander were effective against MCF-7 cells. (phcogres.com)

Chemotherapy , in its most general sense, refers to treatment of disease by chemicals that kill cells, specifically those of micro-organisms or cancer . (wikidoc.org)

BC comprises of variant subtypes and those subtypes arise from basal progenitor or luminal progenitor cells undergoing different genetic mutations[ 2 ]. (ijpsonline.com)
We tested the hypothesis that oxidized low-density lipoprotein (oxLDL)-induced click here inactivation of Akt within endothelial progenitor cells (EPCs) is mediated at the level of Phosphoinositide 3-kinase (PI3K), specifically by nitrosylation of the p85 subunit of PI3K, and that this action is critical in provoking oxLDL-induced EPC apoptosis. (braf-signal.com)

Hairy roots of C. roseus were cultivated in three different types of bioreactors: an air-sparged bioreactor with no stirring, an air-sparged bioreactor in which the medium is slowly circulated through the vessel with a peristaltic pump and a standard air-sparged and stirred bioreactor. (vtt.fi)

M. Valko, D. Leibfritz, J. Moncol T. D. Mark, M. C. Mi- lan and J. Telser, "Free Radicals and Antioxidants in Normal Physiological Functions and Human Disease," The International Journal of Biochemistry and Cell Biol- ogy, Vol. 39, No. 1, 2007, pp. 44-84. (scirp.org)

Catharanthus roseus (L.) G.Don is an accepted species. (stuartxchange.org)

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay was executed for determination of effects of piperine on breast cancer cell viability. (ijpsonline.com)
Clonogenic assay was implemented for checking the effect of piperine on clonogenic potency of breast cancer cells. (ijpsonline.com)
Western blotting assay was implemented to check the levels of phosphatidylinositol 3-kinase and protein kinase B. Results revealed that piperine induced dose reliant cytotoxicity in MCF-7 human breast cancer cells. (ijpsonline.com)
Further, application of piperine on cancer cell colonies remarkably reduced the number of cell colonies to minimum. (ijpsonline.com)
It was reasoned that an agent that damaged the rapidly growing white blood cells might have a similar effect on cancer. (wikidoc.org)
Abrogation of G2 checkpoint specifically sensitize p53 defective cells to cancer chemotherapeutic agents. (mcmaster.ca)
3β-Hydroxylup-20(29)-ene-27,28-dioic acid dimethyl ester (7), isolated from root of Plumbago zeylanica collected from India, also exhibited anti-proliferative and anti-migration activity against triple-negative breast cancer cell lines at IC 50 value of 5 µg/mL [ 7 ] . (encyclopedia.pub)
Vinblastine is a drug used to fight a number of cancers by stopping cancer cell division. (brocku.ca)
Further, this study elucidated the cellular and molecular mechanisms of nanoparticles for anti-proliferative and apoptotic effects on human lung cancer cells (A549) and compared them with commercial drug cisplatin. (springeropen.com)
Cell cycle analysis and downregulation of cyclin D1 indicated cancer cell cessation in the G0/G1 phase. (springeropen.com)
Overall, the results revealed that the green-synthetized AgNPs had anti-metastasis and anti-proliferation effects on lung cancer cells in comparison to cisplatin with lower side effects on the normal cell line. (springeropen.com)
AgNPs are utilized for treating different types of cancerous cells due to their extraordinary biomedical features (anti-cancer, anti-bacterial, anti-viral, and anti-angiogenic properties) as well as surface-specific characteristics (Burdușel et al. (springeropen.com)

The genes for the formation of these active substances are expressed in different cell types. (analytica-world.com)

This revealed increased expression of B-cell lymphoma 2 associated X and reduced expression of B-cell lymphoma 2, indicating apoptosis induction by piperine. (ijpsonline.com)

Root part of C. roseus was extracted in appropriate solvent followed by evaluation of antibacterial activity by agar well diffusion assay against a total of eight bacterial stains. (ijpsr.com)
The root part of Catharanthus roseus was collected from the sandy beaches of Kanyakumari, Tamilnadu, India, in the month of March 2012. (ijpsr.com)

It apparently induces apoptosis in HEK293T cells while at the same time it directly represses the pro-apoptotic Bax protein. (5htreceptor.com)
Notch activator cyclopiazonic acid induces apoptosis in HL-60 cells through calcineurin activation. (go.jp)

Unfortunately, scientists have yet to identify specific features of malignant and immune cells that would make them uniquely targetable (barring some recent examples, such as the Philadelphia chromosome as targeted by imatinib ). (wikidoc.org)
Because of the effect on immune cells (especially lymphocytes), chemotherapy drugs often find use in a host of diseases that result from harmful overactivity of the immune system against self (so-called autoimmunity). (worldsbest.rehab)

Colchicine is a microtubule inhibitor - a drug that prevents the formation of the mitotic spindle, which is an essential process in dividing cells. (acsh.org)

Anatomy3

Organisms5

Diseases1

Chemicals and Drugs14

Phenomena and Processes4

Information Science1

Geographicals1

Alkaloids16

Periwinkle11

Madagascar1

Vincristine3

Biosynthesis6

Leaf6

Vindoline4

Mitosis8

Apocynaceae2

Cultures3

Pathway5

Steenis1

Lochnera2

Anticancer3

Plants2

Alkaloid production1

Gene2

Suspension-cultured1

Enzymes1

Tumor4

Growth6

Cervical cancer1

Plant1

Invasive1

Extracts5

Chemicals1

Progenitor cells2

Hairy1

Biochemistry1

Species1

Cancer12

Genes1

Apoptosis induction1

Root2

Induces apoptosis2

Immune2

Mitotic spindle1