Loading...
  • mammalian cell
  • 15. The isolated cell of claim 14 which is selected from the group consisting of a mammalian cell, an insect cell, a bacterial cell, a yeast cell and a plant cell. (patentsencyclopedia.com)
  • This report describes a comparative study of the susceptibility of one avian and four mammalian cell lines to avian reovirus S1133 and IBDV vaccine GBV-8 strain (Biovet Laboratory) through of presence of cytopathic effect (CPE), virus titration and detection of viral RNA by polyacrylamide gel electrophoresis (PAGE) to better define satisfactory and sensitive cell culture systems for their isolation. (scielo.br)
  • infection
  • isolated memory B cells from people who had survived infection with Andes hantavirus (pictured here). (sciencemag.org)
  • Development of an effective vaccine against EV71 infection is hampered by the lack of appropriate animal models for efficacy testing of candidate vaccines. (biomedcentral.com)
  • Previously, we have successfully tested the immunogenicity and protectiveness of a candidate EV71 vaccine, containing recombinant Newcastle disease virus capsids that display an EV71 VP1 fragment (NPt-VP1 1-100 ) protein, in a mouse model of EV71 infection. (biomedcentral.com)
  • Abortive infection in mammalian cells (no production of progeny viruses) and their ability to accommodate multiple gene inserts are some of the characteristics that make APVs promising vaccine vectors. (biomedcentral.com)
  • Although abortive infection in mammalian cells conceivably represents a major vaccine bio-safety advantage, molecular mechanisms restricting APVs to certain hosts are not yet fully understood. (biomedcentral.com)
  • A continuous bovine kidney cell line, LF-BK, arose from primary bovine calf kidney cells that survived infection with a temperature-sensitive mutant of foot-and-mouth disease virus. (nih.gov)
  • Severe secondary DENV infection was associated with extraordinary peak plasmablast frequencies between 4 and 7 d of illness, averaging 46% and reaching 87% of B cells, significantly greater than those seen in mild illness or primary infections. (jimmunol.org)
  • 70% of IgG-secreting cells in individuals with severe secondary DENV infection were DENV specific. (jimmunol.org)
  • Furthermore, advantage can be taken of a property of the pseudotype VSV, which is competence for single-round infection, in handling many different viruses that are either difficult to amplify in cultured cells or animals or that require specialized containment facilities. (frontiersin.org)
  • Rather, E28 treatment decreased viral load in the serum early in the course of infection, which resulted in blunted dissemination to the brain, an effect that required phagocytic cells, C1q, and FcγRIII (CD16). (asm.org)
  • WNV infection requires attachment to cell surface receptors, which remain poorly defined, endocytosis, and acid-catalyzed fusion of the virus within the late endosome. (asm.org)
  • In the case of cell-free HIV-1, FcγRs have been implicated in both infection enhancement ( 3 ) and virus destruction by phagocytosis ( 4 , 5 , 6 ). (asm.org)
  • Rapid diagnosis of rabies can help initiate prompt infection control and public health measures, obviate the need for unnecessary treatment/medical tests, and assist in timely administration of pre- or postexposure prophylactic vaccination to family members and medical staff. (hindawi.com)
  • The role of T cells in DENV infection and subsequent disease manifestations is not fully understood. (frontiersin.org)
  • According to the original antigenic sin theory, skewing of T-cell responses induced by primary infection with one serotype causes less effective response upon secondary infection with a different serotype, predisposing to severe disease. (frontiersin.org)
  • Virus yields from CEF and cell lines infection with both viruses was determined in the CEF culture. (scielo.br)
  • CPE of reovirus infection was characterized by formation of syncytia followed by degeneration leaving holes in the monolayer and giant cells floating in the medium. (scielo.br)
  • Laboratory
  • Assists in a laboratory by preparing culture media used to develop vaccines and toxoids or to conduct chemical, microscopic, and bacteriologic tests. (erieri.com)
  • The main focus of the laboratory is the molecular and cell biology of virus-host interactions. (icgeb.org)
  • Reports that Trimethoprim® plus Sulfalene® are an effective cure for Plasmodium falciparum malaria in man suggested a laboratory study of these drugs to aid in evaluating tests with animals in the search for new antimalarials. (ajtmh.org)
  • receptors
  • Coencapsulation of T cells and target cells into arrays of subnanoliter droplets improves the identification of functional T cell receptors. (sciencemag.org)
  • However, receptors or co-receptors of several viruses have been difficult to identify because of the lack of reliable cell culture systems, an insufficient amount of native viral particles, or difficulty in handling because of the requirement for biosafety level (BSL)-3 or -4 containment. (frontiersin.org)
  • Many cells of the immune system express Fc receptors that bind immune complexes through the constant region of immunoglobulins to mediate a variety of innate and acquired immune defense mechanisms ( 1 , 2 ). (asm.org)
  • To study the impact of the four major Fcγ receptors on cell-free HIV-1 in greater detail, we stably expressed each of the cDNAs for the human FcγRs on TZM-bl cells. (asm.org)
  • vectors
  • Targeting vectors pseudotyped with distinct viral envelope proteins that influence cell tropism and transfection efficiency are useful tools not only for examining entry mechanisms or cell tropisms but also for vaccine vector development. (frontiersin.org)
  • Purified soluble envelope proteins, viral-like particles which are produced in insect cells by baculoviral vectors, and authentic viral particles obtained from patients have been used to study the mechanisms of viral attachment and to identify binding receptor molecules. (frontiersin.org)
  • titers
  • In the presence of FcγRI expression, neutralization titers for 4E10 and 2F5 were increased as much as 5,000-fold depending on the virus used. (asm.org)
  • CPE and viral RNA were detected in CER and BHK-21 cells after reovirus inoculation and in RK-13 cell line after IBDV inoculation and with high virus titers. (scielo.br)
  • augment
  • M-CSF-induced Mphi produced low levels of IL-10 constitutively and high levels after stimulation with LPS, but neutralization of IL-10 did not augment Mphi IL-12 production. (ox.ac.uk)
  • This mechanism is supported by the finding that cell-free HIV-1 immune complex formation was not required prior to Ab-FcγRI engagement in order for FcγRI to augment the neutralization activities of 2F5 and 4E10 ( 7 ). (asm.org)
  • cultures
  • Avian reoviruses and infectious bursal disease virus (IBDV) are usually isolated and grown in embryonated eggs and/or in primary avian cell cultures (5, 13, 17, 18). (scielo.br)
  • Cell cultures. (scielo.br)
  • The serial 10-fold dilutions of each cell cultures-virus supernatants were prepared in growth medium and 50 µl of each dilution were transferred to each of the four wells of a 96-well microtiter plate that contained the same volume of fresh CEF suspensions (3.0 x 10 5 cells/ml). (scielo.br)
  • bovine
  • The cell line and primary bovine kidney cells were less susceptible than primary bovine thyroid cells to several subtypes of the virus in suspensions of tongue epithelium. (nih.gov)
  • a positive intradermal test for M. bovis was obtained in two contact-infected calves by different bovine PPD tuberculins based on M. bovis bacillus Calmette-Guerin (BCG) secreted or somatic antigens. (univ-amu.fr)
  • 2002
  • Amor KB, Breeuwer P, Verbaarschot P, Rombouts FM, Akkermans AD, De Vos WM, Abee T (2002) Multiparametric flow cytometry and cell sorting for the assessment of viable, injured, and dead bifidobacterium cells during bile salt stress. (univ-amu.fr)
  • pathogenesis
  • The present rheologic study was performed in monkeys infected by Plasmodium coatneyi in order to assess further the role of abnormal red cell deformability in the pathogenesis of disease. (ajtmh.org)
  • Herein, we will discuss the role of T cells in protection and pathogenesis from severe disease as well as the implications for vaccine design. (frontiersin.org)
  • immune
  • 30. A method of generating an immune response in a subject, comprising transfecting the gene delivery vector of claim 17 into cells of the subject under conditions suitable for expression of the polynucleotide and production of the polypeptide in the subject, wherein an immunological response to the polypeptide is elicited in the subject. (patentsencyclopedia.com)
  • A previous analysis ( 20 ) documented the substantial knowledge gaps existing at the level of defined T- and B-cell immune responses. (frontiersin.org)
  • viral
  • This narrow time window led to limitations in the testing of various parameters for vaccination and viral challenge. (biomedcentral.com)
  • During the past few years, the lab, in addition to photobleaching techniques, has developed a unique method to visualize viral RNAs in real time in living cells. (icgeb.org)
  • serum
  • Serum samples of 20 patients with bladder cancer and 17 patients with renal cancer without neurological disorders as well as of 49 healthy controls and 39 patients with other known antineuronal autoantibodies (anti-NMDAR, anti-Hu, anti-Yo, anti-Ri, anti-AQP4, anti-LGI1, anti-CASPR2, anti-GAD65) were tested as controls. (biomedcentral.com)
  • Cells were grown in Eagle's minimum essential medium (MEM) containing 8% fetal calf serum (FCS). (scielo.br)
  • significantly
  • Results After A549/DDP co-culture with CIK cells, the DDP resistance of A549/DDP significantly decreased in a time-dependent manner. (jcancer.org)
  • The secretion of interferon (IFN)- γ significantly increased along with the co-culture time of A549/DDP with CIK cells. (jcancer.org)
  • line
  • Objective This study aimed to evaluate the effects of CIK cells on cisplatin (DDP) resistance in the human lung adenocarcinoma cell line A549/DDP. (jcancer.org)
  • HeLa cells, from which the TZM-bl cell line was developed, are known to exhibit properties of nonprofessional phagocytes ( 9 , 10 , 11 ). (asm.org)
  • The RK-13 cell line was the only one that presented CPE after IBDV inoculation, while the CER and BHK-21 cells were sensible to the reovirus ( Table 1 ). (scielo.br)
  • methods
  • Since March 2006 to February 2007, 86 specimens from Sistan & Balouchestan,63 specimens from Tehran and 48 samples from Fars sewage disposal systems and surface water were collected by Grab Sample method and tested for enteroviruses directly by using two concentration methods: Pellet and Two-phase. (biomedcentral.com)
  • This long case definition aims to describe the methods presently available for screening specimens and confirming the identity of STEC and to provide guidance for laboratories, which might wish to introduce tests for STEC. (health.gov.au)
  • laboratories
  • However this method is slow and only available to laboratories with cell culture facilities. (health.gov.au)
  • Finally, chemical compounds synthesized in collaborating laboratories are tested for antiviral activity. (icgeb.org)
  • positive
  • On these samples, TB complex-specific polymerase chain reaction (PCR) tests for IS6110 were also positive, albeit with a different time kinetics. (univ-amu.fr)
  • partially
  • The effect of CIK cells on re-sensitizing lung cancer cells to the chemotherapy drug was partially dependent on the secretion of IFN-γ. (jcancer.org)
  • surface
  • Thus, surface expression of FcγRs may, in principle, turn TZM-bl cells into professional phagocytes. (asm.org)
  • effect
  • We showed that expression of FcγRI/CD64 had a profound effect on HIV-1 neutralization by the gp41 membrane-proximal external region (MPER)-specific MAbs 2F5 and 4E10 ( 7 ). (asm.org)