• Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. (nature.com)
  • We found that the total transcript level per cell and the expression of most individual genes correlated with progression through the cell cycle, but not with cell size. (frontiersin.org)
  • The molecular processes behind cell cycle progression have been dissected by numerous morphological studies on live or fixed single cells using a plethora of techniques to visualize components and processes during cell division. (frontiersin.org)
  • Epidemiologic, animal, and laboratory studies suggest that 5-amino-salicylic acid (5-ASA) protects from the development of CRC by altering cell cycle progression and by inducing apoptosis. (nih.gov)
  • In this study, we hypothesized that 5-ASA restrains cell cycle progression by activating checkpoint pathways in colorectal cell lines, which would prevent tumor development and improve genomic stability. (nih.gov)
  • Cell cycle progression, phosphorylation, and DNA binding of cell cycle checkpoint proteins were analyzed. (nih.gov)
  • We found that 5-ASA at concentrations between 10 and 40 mmol/L affects cell cycle progression by inducing cells to accumulate in the S phase. (nih.gov)
  • Progression of a cell through the division cycle is tightly controlled at different steps to ensure the integrity of genome replication and partitioning to daughter cells. (ebi.ac.uk)
  • While several studies addressed how cell motility parameters change throughout the cell cycle, the relationship between invadopodia and cell cycle progression has not been elucidated. (biorxiv.org)
  • Cell cycle progression is faster in cells in which invadopodia are abolished (by Tks5 knockdown), evidenced by earlier induction of cyclins A and B. A close look at the regulators of G1 revealed that the overexpression of p27 kip1 , but not p21 cip1 , causes faster turnover of invadopodia and increased ECM degradation. (biorxiv.org)
  • Taken together, these findings suggest that invadopodia function is tightly linked to cell cycle progression and is controlled by specific cell cycle regulators. (biorxiv.org)
  • Cell cycle progression is governed by a complex network of cyclin-dependent kinases that define not only the phase of the cell cycle, but also the timing of transitions between phases 13 . (biorxiv.org)
  • In addition, we found that HBX introduction downregulated miR-18a, upregulated CTGF, elevated the viability, and promoted cell cycle progression. (medscimonit.com)
  • Proliferation and cell cycle progression of the serum-free cultures closely mimicked that of serum-dependent cells. (lu.se)
  • These findings establish a mechanism for co-targeting DNA damage-induced cell cycle checkpoints in combination with repair of cisplatin-DNA lesions in vivo using RNAi nanocarriers, and motivate further exploration of ASL as a generalized strategy to improve cancer treatment. (nature.com)
  • Cell proliferation includes a series of events that is tightly regulated by several checkpoints and layers of control mechanisms. (frontiersin.org)
  • Many more investigations have been made on cells, sorted according to size, or artificially arrested at various cell cycle checkpoints. (frontiersin.org)
  • To ensure the proper replication of cellular components and division, there are control mechanisms known as cell cycle checkpoints after each of the key steps of the cycle that determine if the cell can progress to the next phase. (wikipedia.org)
  • d) investigation of cell-cycle checkpoints and replicative responses to the changing environment in the human host and to antimalarial drugs. (europa.eu)
  • While BRCA2 expression is involved in cell cycle checkpoints and DNA repair, the mechanisms of cell cycle-dependent regulation of BRCA2 gene expression remains elusive. (biomedcentral.com)
  • is known to exert anticancer effects, such as inducing cell cycle arrest, inhibiting metastasis, and overcoming immunotherapy resistance in breast cancer cells. (hindawi.com)
  • Flow cytometry analysis showed that VALD-3 triggered cell cycle arrest and induced apoptosis of breast cancer cells. (researchsquare.com)
  • Eidukevicius R, Characiejus D, Janavicius R, Kazlauskaite N, Pasukoniene V, Mauricas M, Den Otter W: A method to estimate cell cycle time and growth fraction using bromodeoxyuridine-flow cytometry data from a single sample. (biomedcentral.com)
  • The cell cycle distribution was analyzed through PI staining and flow cytometry analysis, while apoptotic cells were measured by double staining with Annexin V-FITC and PI. (biomedcentral.com)
  • Standard techniques like cell synchronization by serum starvation, flow cytometry, N-terminal or C-terminal FLAG epitope-tagged protein expression, immunofluorescence confocal microscopy, dual luciferase assay for promoter evaluation, and chromatin immunoprecipitation assay were employed during this study. (biomedcentral.com)
  • The distribution of the cell cycle was analyzed by flow cytometry. (fluoridealert.org)
  • Cell viability was determined using Cell Counting Kit-8 assay. (biomedcentral.com)
  • Straub TM , Honer zu Bentrup K , Orosz-Coghlan P , Dohnalkova A , Mayer BK , Batholomew RA , In vitro cell culture assay for human noroviruses. (cdc.gov)
  • The specific aim of our project was to develop an in vitro cell culture infectivity assay for human norovirus (hNoV) to enhance risk assessments when these viruses are detected in water supplies. (cdc.gov)
  • This study is the first to show that VALD-3 induces apoptosis and cell cycle arrest in human breast cancer cells by suppressing Wnt/β-catenin signaling, indicating that it could be a potential drug for the treatment of breast cancer. (researchsquare.com)
  • Most changes were subtle, but frequently the genes with altered expression were involved in basal cellular functions such as cell division, cell cycle regulation, apoptosis and cell signalling. (lu.se)
  • The expression of cyclin-dependent kinase inhibitors was profoundly affected with early activation and then repression of p21 cip1/waf1 and persistent activation of both p27 kip1 and p57 kip2 , whereas genes involved in cell survival and proliferation were suppressed. (aacrjournals.org)
  • Similarly, depletion in the STRIPAK component STRIP1 affects activation of GCKIII kinases and cell cycle disruption through elevated expression of cyclin dependent kinase inhibitors p21 and p27, enhanced levels of which lead to a protective effect from therapeutic treatments and increased proliferation. (lu.se)
  • In response to DNA damage, a synthetic lethal relationship exists between the cell cycle checkpoint kinase MK2 and the tumor suppressor p53. (nature.com)
  • In recent years, cell cycle regulators have been shown to exhibit roles in both tumor suppression and tumor promotion, particularly cyclin-dependent kinase inhibitors (CKI) p27 kip1 and p21 cip1 14 . (biorxiv.org)
  • It has also been demonstrated that TGF-β induced cell cycle arrest can be partially attributed to the regulatory effects of TGF-β on both the expression and activity of cyclin-dependent kinase inhibitors [CDKI] such as p21 and p27. (biomedcentral.com)
  • At least 1/10 of breast cancers and colorectal cancers occur because of heredity and recently the cell cycle kinase 2, CHEK2 1100delC allele has been identified at a particularly high frequency in families with hereditary breast and colorectal cancer. (lu.se)
  • 2010). Polycyclic aromatic hydrocarbon exposure standard mammogram form and the interactive kinase in response to estradiol in breast cancer in oesophageal tissue and risk of oesophageal threshold measurement methods. (who.int)
  • SL originally described a relationship between two genes, where alteration of either gene alone results in viable cells, but alteration (mutation, loss, or inhibition) of both genes simultaneously was lethal. (nature.com)
  • Because BRCA mutations are observed in fewer than 10% of cancer patients (cBioPortal: 6.7%) 11 , 12 , 13 the identification of additional genes that share synthetic lethal sensitivity relationships with mutated oncogenes or tumor suppressors would greatly enhance the implementation of tumor cell-specific synthetic lethal sensitivity to improve an anticancer therapeutic response. (nature.com)
  • The intersection between the differentially expressed genes (DEGs) and the HNK-targets was determined using a Venn diagram, and the results were analyzed using a protein-protein interaction network, hub gene selection, gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses, genetic alteration analysis, survival rate, and immune cell infiltration levels. (hindawi.com)
  • Here, we used quantitative real-time PCR, profiling the expression of 93 genes in single-cells from three different cell lines. (frontiersin.org)
  • Detailed analysis of cell cycle predictive genes allowed us to define subpopulations with distinct gene expression profiles and to calculate a cell cycle index that illustrates the transition of cells between cell cycle phases. (frontiersin.org)
  • In conclusion, we provide useful experimental approaches and bioinformatics to identify informative and predictive genes at the single-cell level, which opens up new means to describe and understand cell proliferation and subpopulation dynamics. (frontiersin.org)
  • Here, we employed single-cell gene expression profiling to describe the dynamic transition between cell proliferative states in three different cell lines using a panel consisting of 93 marker genes. (frontiersin.org)
  • This method identifies cooperative module pairs that significantly influence a cellular process and the correlated genes and interactions that are essential to that process. (biomedcentral.com)
  • DUSP4 alterations lead to hyperactivation of MAPK signaling in many cancers, including breast cancer, which often harbor mutations in cell cycle checkpoint genes, particularly in TP53. (biomedcentral.com)
  • Interestingly, replication stress in Brca2-null cells activates p53 and the expression of its target genes, including senescence-inducing Ink4/Arf. (biomedcentral.com)
  • Some alterations were restored when the cells were transferred back to serum-containing medium, indicating that expression of these genes was controlled by components in serum. (lu.se)
  • Moreover, some studies demonstrated that LAPTM5 was highly expressed in malignant B lymphomas and involved in B cell malignancies ( 10 ), involving in negative regulation of cell surface T and B cell receptor by promoting lysosome degradation ( 6 ). (spandidos-publications.com)
  • A Data-Driven, Mathematical Model of Mammalian Cell Cycle Regulation. (ebi.ac.uk)
  • To develop a data-driven model of cell cycle regulation, we used contiguous, dynamic measurements over two time scales (minutes and hours) calculated from static multiparametric cytometry data. (ebi.ac.uk)
  • The tumour microenvironment (TME) contains several other cell types apart from cancer cells which play a role not only in the regulation of the environment but in response to treatments. (lu.se)
  • Taken together, our results suggested that decreased LAPTM5 inhibited proliferation and viability, as well as induced G0/G1 cell cycle arrest possibly via deactivation of ERK1/2 and p38 in BCa cells. (spandidos-publications.com)
  • Learn about cell viability assays. (abcam.com)
  • Cell viability was detected by CCK-8 method. (fluoridealert.org)
  • Furthermore, we investigated the effect of HBX overexpression on miR-18a and CTGF, as well as the viability and cell cycle status of HepG2 cells. (medscimonit.com)
  • We transfected HepG2.2.15 with miR-18a mimics and CTGF siRNA, finding that upregulated miR-18a and downregulated CTGF suppress the viability and cause cell cycle arrest. (medscimonit.com)
  • 1 ). By using 3-dimensional aggregates of a highly differentiated intestinal epithelial cell line, the investigators claimed to have established an in vitro cell culture model that "support[s] the natural growth of human noroviruses. (cdc.gov)
  • The utility of epithelial-cell micronuclei in the assessment of intermittent exposures. (cdc.gov)
  • Epithelial-cell micronuclei (MN) are potentially useful markers of occupational exposure to genotoxicants. (cdc.gov)
  • Results and Conclusions: The adaptation process was characterized by initial cell population growth. (lu.se)
  • Results and Conclusions: The adaptation process was characterized by initial cell population growth arrest, and after that extensive cell death, followed by proliferation and long-term survival of clonal cultures. (lu.se)
  • Therefore, compared to normal p53-proficient cells, p53-defective cells are more reliant on MK2 activity, which drives an alternative cell cycle checkpoint pathway that stabilizes the CKI inhibitors p27 Kip1 and Gadd45α in order to maintain G 1 /S and G 2 /M arrest after certain types of DNA damage 16 , 18 . (nature.com)
  • VALD-3 also regulated the Wnt/β-catenin signaling pathway in breast cancer cells, inhibiting the activation of downstream molecules. (researchsquare.com)
  • Growth factors do not only stimulate cell proliferation, but they may also act as growth inhibitors, depending on the cell type and the stimulatory pathway that is involved. (biomedcentral.com)
  • Because APE1/Ref-1 is expressed and elevated in prostate cancer, we sought to characterize APE1/Ref-1 expression and activity in human prostate cancer cell lines and determine the effect of selective reduction-oxidation (redox) function inhibition on prostate cancer cells in vitro and in vivo . (oncotarget.com)
  • An image depicting head and neck squamous cell carcinoma in vitro can be seen below. (medscape.com)
  • Head and neck squamous cell carcinoma in vitro (cell culture). (medscape.com)
  • Indeed, in vitro cell culture infectivity assays for the waterborne pathogen Cryptosporidium parvum that supplement approved fluorescent microscopy assays do not result in amplification of the environmentally resistant hard-walled oocysts ( 2 ). (cdc.gov)
  • s assertion regarding the suitability of our method for the in vitro propagation of high titers of hNoV is valid for the medical research community. (cdc.gov)
  • Objective: Serum is usually added to growth media when mammalian cells are cultured in vitro to supply the cells with growth factors, hormones, nutrients and trace elements. (lu.se)
  • Cadi, R. dc.description.abstract: Background: The success of in vitro-fertilization (IVF) cycles is determined in large part by the quality of embryo cleavage, which in turn, is dependent on the quality of the embryo culture media (CM). Many factors can influence the quality of embryo CM, one of which is the levels of Cell Free Deoxyribonucleic acid (DNA). (who.int)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • These results provide a global view of the cell cycle and the method can be used to discover the dynamic coordination properties of functional components in other cellular processes. (biomedcentral.com)
  • The notion could be that unique cellular mechanisms are triggered in the breast cancer cells to stimulate BRCA2 gene expression as a temporary measure to regulate the growth of the breast cancer cells. (biomedcentral.com)
  • We found that Dusp4 loss alone is insufficient in mediating tumorigenesis, but alternatively converges with loss in Trp53 and MYC amplification to induce tumorigenesis primarily through chromosome 5 amplification, which specifically upregulates Dbf4 , a cell cycle gene that promotes cellular replication by mediating cell cycle checkpoint escape. (biomedcentral.com)
  • This study identifies a novel mechanism for breast tumorigenesis implicating Dusp4 loss and p53 mutations in cellular acquisition of Dbf4 upregulation as a driver of cellular replication and cell cycle checkpoint escape. (biomedcentral.com)
  • This is the multihit theory of tumorigenesis, in which a series of multiple triggering events in the genetic and cellular makeup of a cell ultimately cause cancer. (medscape.com)
  • Polarization is a fundamental cellular property that plays a vital role in various biological processes in multi-cellular as well as single-cell organisms. (elifesciences.org)
  • Breast cancer stem cells (BCSCs) play a critical role in chemoresistance, metastasis, and poor prognosis of breast cancer. (hindawi.com)
  • Analysis of the changes of migration and invasion, showed significant reduced LAPTM5 suppressed cell metastasis. (spandidos-publications.com)
  • Tumor cell invasion and metastasis require cancer cells to be both proliferative and invasive, i.e. migrate through the tissue and assemble invadopodia. (biorxiv.org)
  • Metastasis is a complex, multi-step process that is initiated when cancer cells in the primary tumor acquire invasive properties, including motility and the ability to breakdown the extracellular matrix (ECM) 1 , and is responsible for the majority of cancer-related mortalities 2 . (biorxiv.org)
  • Recent studies denoted that alterations in growth factors and growth factor receptors seem to influence the biologic behaviour of pancreatic cancer cells [ 2 ]. (biomedcentral.com)
  • Cell Cycle Arrest and microRNA Signatures in Pancreatic Adenocarcinoma Cells. (bvsalud.org)
  • Effect of Aspirin on G 0 /G 1 Cell Cycle Arrest and microRNA Signatures in Pancreatic Adenocarcinoma Cells. (bvsalud.org)
  • This study aimed to examine the antitumour effects and possible fundamental mechanisms of aspirin in pancreatic cancer cells . (bvsalud.org)
  • Aspirin inhibited cell proliferation in pancreatic cancer cell lines and induced G0/ G1 cell cycle arrest by decreasing the expression of cyclin D1 . (bvsalud.org)
  • Aspirin inhibited the proliferation of pancreatic cancer cells and induced cell cycle arrest . (bvsalud.org)
  • Cancer Lett, 300:215-224.doi:10.1016/j. squamous cell carcinoma in north-eastern Iran. (who.int)
  • Extracts prepared from Trametes versicolor under the trade name of I'm-Yunity exhibit anti-tumorigenic activities, as supported by inhibition of the proliferation and induction of apoptosis in malignant cells. (spandidos-publications.com)
  • Due to the role of oncogenic transcriptional activators NFĸB and STAT3 in survivin protein expression, and APE1/Ref-1 redox activity regulating their transcriptional activity, we assessed selective inhibition of APE1/Ref-1's redox function as a novel method to halt prostate cancer cell growth and survival. (oncotarget.com)
  • Our study demonstrates that survivin and APE1/Ref-1 are significantly higher in human prostate cancer specimens compared to noncancerous controls and that APE1/Ref-1 redox-specific inhibition with small molecule inhibitor, APX3330 and a second-generation inhibitor, APX2009, decreases prostate cancer cell proliferation and induces cell cycle arrest. (oncotarget.com)
  • Most of the identified module pairs cover cooperative pathways and components essential to the cell cycle. (biomedcentral.com)
  • We appraised the antitumour effects of aspirin on cell proliferation and tumour growth , cell cycle distribution, apoptosis , signalling pathways, angiogenesis-related proteins , and phosphorylated receptor tyrosine kinases (p-RTKs) and identify miRNAs associated with its antitumour effects. (bvsalud.org)
  • Quantification of cell-cycle state at a single-cell level is essential to understand fundamental three-dimensional (3D) biological processes such as tissue development and cancer. (nature.com)
  • We applied our workflow to quantify cell-cycle effects of three antimitotic cancer drugs over 8 d in HT-1080 fibrosarcoma xenografts in living mice using a data set of 38,000 cells and compared the induced phenotypes. (nature.com)
  • Cancer cells that are defective in p53 function are deficient in their ability to transcriptionally upregulate the CDK inhibitor p21 after genotoxic stress. (nature.com)
  • In this study, by comparing the activity of normal cell lines and cancer cell lines after treating with resveratrol, it was found that resveratrol has more significant cytotoxicity in cancer cell lines. (mdpi.com)
  • Resveratrol could play a toxic role through inducing apoptosis of the cancer cell in a time- and concentration-dependent manner. (mdpi.com)
  • Chemoresistance, or the insensitivity of cancer cells to drug therapy, is a major factor in the failure of chemotherapy against breast cancer. (hindawi.com)
  • Breast cancer stem cells (BCSCs) are one of the main factors driving chemoresistance, thereby contributing to poor prognosis and clinical outcomes [ 3 - 5 ]. (hindawi.com)
  • Our transcriptome analysis revealed in bladder cancer (BCa) tissues a significant induction of lysosomal-associated multispanning membrane protein 5 (LAPTM5), a lysosomal membrane protein preferentially expressing in immune cells and hematopoietic cells. (spandidos-publications.com)
  • Furthermore, previous studies suggested that knockdown of LAPTM4B , another important subtype of the LAPTM family inhibited proliferation of hepatocellular carcinoma ( 11 ), prostate ( 12 ) and breast cancer cells ( 13 ). (spandidos-publications.com)
  • I'm-Yunity™ is a proprietary method procured version of the Basidomycetes mushroom comprising a heterogeneous family of polysaccharide-protein complexes isolated from the cultivated mycelia of Trametes (formerly Coriolus ) versicolor , which have been used as a major ingredient in traditional Chinese medicinal formulations for the prevention and treatment of chronic diseases including cancer ( 18 - 27 ). (spandidos-publications.com)
  • In this study, we investigated the cytotoxic and apoptotic effects of VALD-3, a Schiff base ligand synthesized from o-vanillin derivatives, on human breast cancer cells and the possible underlying mechanisms. (researchsquare.com)
  • 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-test was used to observe the proliferation of human breast cancer MCF-7 and MDA-MB-231 cells induced on VALD-3. (researchsquare.com)
  • By xenografting human breast cancer cells into nude mice, we found that VALD-3 significantly suppressed tumor cell growth while showing low toxicity against major organs. (researchsquare.com)
  • In our article in BMC Cancer [ 1 ] we modelled our graph for the passage of bromodeoxyuridine-labelled cells through the cell cycle presented in Figure 1 on the figure published by Johansson et al. (biomedcentral.com)
  • Invadopodia are cancer cell protrusions rich in structural proteins (e.g. (biorxiv.org)
  • Deregulated cell cycle is a hallmark of cancer 12 . (biorxiv.org)
  • While studying the activity of BRCA2 gene promoter in breast cancer cells, we discovered that this promoter has bi-directional activity and the product of the reverse activity (a ZAR1-like protein, we named ZAR2) silences the forward promoter at the G0/G1 phase of the cell. (biomedcentral.com)
  • Using a genetically engineered mouse model, we generated mammary-specific Dusp4 -deleted primary epithelial cells to investigate the necessary conditions in which DUSP4 loss may drive breast cancer oncogenesis. (biomedcentral.com)
  • Given the previously reported role of DUSP4 as a p53 target, a potential cell cycle checkpoint, and in mediating senescence downstream of replication stress, we hypothesized that DUSP4 may be a critical oncogenic driver in breast cancer. (biomedcentral.com)
  • In turn, as a result of research into the abnormal cancer cell, the basic understanding of the cell has greatly improved. (medscape.com)
  • Cancer is characterized by uncontrolled growth and division of a cell, with extension beyond the normally limiting basement membrane and through the boundaries of normal cells. (medscape.com)
  • [ 6 ] These events lead the cancer cell to escape normal cell growth and control mechanisms, to avoid system control mechanisms (ie, immunologic surveillance), and to establish a nutrient supply. (medscape.com)
  • In this study, we explore mechanisms in breast cancer cells that potentially lead to recurrence. (lu.se)
  • Both of these altered proteins lead to the eventual ability of cancer cell recurrence. (lu.se)
  • We explore signalling alterations resulting in cancer stem cells as well as cell cycle arrest and cell fate determination. (lu.se)
  • Methods: We utilized the Southern Sweden population-based cancer registry to identify women with double primary breast and colorectal cancer and sequenced tumor material in order to assess the contribution of the CHEK2 1100delC to the development of such metachronous tumors. (lu.se)
  • 99:209 -215.doi:10.1007/s11060 - 010 - 0129 -5 cell carcinoma-a population-based study in with and without cervical cancer in Tbilisi, Georgia. (who.int)
  • However, the molecular mechanisms of action of HNK in BCSCs, as well as its effects on the cell cycle, remain unclear. (hindawi.com)
  • This study aimed to explore the potential targets and molecular mechanisms of HNK on metastatic BCSC (mBCSC)-cell cycle arrest and the impact of the TME. (hindawi.com)
  • These results are consistent with previous studies and demonstrate that our methodology is effective for studying cooperative mechanisms in the cell cycle. (biomedcentral.com)
  • The model accounts for important details of the physiology, biochemistry, and genetics of cell cycle control in stalked C. crescentus cell. (ebi.ac.uk)
  • Because of its mutated aggressive genetics, this cell has a selective growth advantage over its neighbors. (medscape.com)
  • Frontiers in cell and developmental biology. (lu.se)
  • LAPMT5 is a lysosomal membrane protein preferentially expressed in immune cells ( 5 , 6 ) and hematopoietic cells ( 7 ), having a close interaction with the Nedd4 ( 8 ), a member of the E3 ubiquitin ligases family ( 8 ). (spandidos-publications.com)
  • It reproduces protein time courses in wild-type cells, mimics correctly the phenotypes of many mutant strains, and predicts the phenotypes of currently uncharacterized mutants. (ebi.ac.uk)
  • The alkaline phosphatase (ALP) activity and bone Gla protein (BGP) content were detected by micronutrient enzymes standard method and ELISA. (fluoridealert.org)
  • Further, the results of miR-4755-5p mimic transfection confirmed that under the action of NaF, miR-4755-5p overexpression reduced Cyclin D1 protein expression within osteoblasts and further inhibited cell proliferation and activation. (fluoridealert.org)
  • In that study investigators failed to observe an increase in viral shedding from symptomatic piglets upon serial passage, despite successful intracellular detection of viral RNA and newly synthesized virus-encoded protein in host cells dying of apoptosis. (cdc.gov)
  • WBV exposure resulted in a significant reduction in number of developing follicles, and decrease in circulating estradiol concentrations, ovarian luteinizing hormone receptor protein levels, and marked changes in transcript levels for several factors involved in follicular development, cell cycle, and steroidogenesis. (cdc.gov)
  • In this compiled work, we elaborate on the effect of protein expression alterations and their resulting effect on recurrence capability of cells. (lu.se)
  • It combines image analysis and machine learning methods for automated 3D segmentation and cell-cycle state identification of individual cell-nuclei with widely varying morphologies embedded in complex tumor environments. (nature.com)
  • Figure 2: Automatic segmentation of cell nuclei. (nature.com)
  • In cells with nuclei (eukaryotes, i.e., animal, plant, fungal, and protist cells), the cell cycle is divided into two main stages: interphase and the mitotic (M) phase (including mitosis and cytokinesis). (wikipedia.org)
  • In cells without nuclei (prokaryotes, i.e., bacteria and archaea), the cell cycle is divided into the B, C, and D periods. (wikipedia.org)
  • In schizogony a single parasite first generates many nuclei via independent, asynchronous rounds of genome replication, prior to cytokinesis which is the physical division of the cell. (europa.eu)
  • Timecourses have been conducted to quantify the number and type of replicating nuclei, together with other cell-biological features, in P. falciparum parasites across the course of both erythrocytic schizogony and gametogenesis. (europa.eu)
  • Our data demonstrate that 5-ASA causes cells to reversibly accumulate in S phase and activate an ATR-dependent checkpoint. (nih.gov)
  • We find that together with loss-of-function mutations in p53 and, to some degree, potentiated by cMyc overexpression, Dusp4 deletion aids in cell cycle checkpoint escape while simultaneously potentiating hallmarks of replicative stress, including multinucleation and Chk1 phosphorylation. (biomedcentral.com)
  • Our previous results indicate that 5-ASA improves replication fidelity in colorectal cells, an effect that is active in reducing mutations. (nih.gov)
  • These events include the duplication of its DNA (DNA replication) and some of its organelles, and subsequently the partitioning of its cytoplasm, chromosomes and other components into two daughter cells in a process called cell division. (wikipedia.org)
  • The B period extends from the end of cell division to the beginning of DNA replication. (wikipedia.org)
  • The D period refers to the stage between the end of DNA replication and the splitting of the bacterial cell into two daughter cells. (wikipedia.org)
  • Motile, piliated swarmer cells differentiate into stalked cells at the G1-S transition by shedding their polar flagellum, growing a stalk at that site, losing the polar pili, and initiating DNA replication. (harvard.edu)
  • CtrA is present in the shaded cells, where it represses DNA replication initiation and is cleared by proteolysis during the swarmer cell-stalked cell (G1-S) transition. (harvard.edu)
  • Malaria parasites replicate inside the cells of their human host via 'schizogony', which is fundamentally different from conventional binary fission - the replication mode used by most cells from human cells to yeast cells. (europa.eu)
  • This project is elucidating the spatio-temporal dynamics of DNA replication in these contrasting cell cycles. (europa.eu)
  • To do this, I have developed a method for labelling nascent DNA replication, which was not hitherto possible in Plasmodium. (europa.eu)
  • Aim 1: detailed characterisation, at the whole-cell level, of the asynchronous genome replication that occurs in schizogony and gametogenesis. (europa.eu)
  • While the authors provide compelling evidence of successful virus infection through microscopy, hybridization of viral RNA after 5 passages in cell culture, and preliminary evidence of viral RNA replication through limiting dilution PCR, we question the level of virus replication that is actually achieved in this system. (cdc.gov)
  • This suggests that the demonstration of cytopathic effect and virus internalization in cells alone may not provide direct evidence of productive virus replication. (cdc.gov)
  • Here, we present an integrated workflow for quantitative in vivo cell-cycle profiling. (nature.com)
  • Analysis of mitosis and antimitotic drug responses in tumors by in vivo microscopy and single-cell pharmacodynamics. (nature.com)
  • Single-cell and subcellular pharmacokinetic imaging allows insight into drug action in vivo . (nature.com)
  • In this study, using invadopodia- and cell cycle- fluorescent markers, we show in 2D and 3D cell cultures, as well as in vivo , that breast carcinoma cells assemble invadopodia and invade into the surrounding ECM preferentially during the G1 phase of the cell cycle. (biorxiv.org)
  • The in vivo effects of β-peltatin and podophyllotoxin were evaluated on a subcutaneously-xenografted BxPC-3 cell nude mice model. (biomedcentral.com)
  • ZAR2 binds to BRCA2/ZAR2 bi-directional promoter in vivo and is responsible, at least in part, for the silencing of BRCA2 gene expression in the G0/G1 phase in human breast cells. (biomedcentral.com)
  • It has been reported that TGF-β influence different cell functions, including growth, proliferation and differentiation. (biomedcentral.com)
  • This dynamic oscillatory behavior is regulated by the activity of ECM receptor integrin β1, which determines the length of time a cell spends in each state. (biorxiv.org)
  • Erythropoietin is a glycoprotein hormone that stimulates red blood cell (RBC) production in bone marrow via binding to the cell-surface receptor on hematopoietic progenitor cells, and it has been widely used for treating anemia [ 13 ]. (hindawi.com)
  • Silencing of the EBF3 locus was observed in brain, colorectal, breast, liver, and bone tumor cell lines and its reactivation was achieved on treatment with 5-aza-2′-deoxycytidine and trichostatin A in a significant portion of these tumor cells. (aacrjournals.org)
  • Laub MT, McAdams HH, Feldblyum T, Fraser CM, Shapiro L. Global analysis of the genetic network controlling a bacterial cell cycle. (harvard.edu)
  • By assaying their anti-proliferative and anti-apoptotic effects using human promyelocytic HL-60 cells, we found that the ethanolic extract of I'm-Yunity-Too was more active in inducing cell death compared to I'm-Yunity, based on measured changes in the expression of caspase 3 and Bax. (spandidos-publications.com)
  • Remarkably little is known about Plasmodium cell cycles, despite a wealth of knowledge on the subject in human cells. (europa.eu)
  • This takes a period of ~24 hours when the parasite is inside human red blood cells. (europa.eu)
  • This technologic and biologic revolution continued through the 1960s to today, and the explosion in technology has fueled the current expansion of knowledge into the working of the human cell. (medscape.com)
  • Non-proliferative (non-dividing) cells in multicellular eukaryotes generally enter the quiescent G0 state from G1 and may remain quiescent for long periods of time, possibly indefinitely (as is often the case for neurons). (wikipedia.org)
  • I don't understand how you get 1000 cycles above 80% 100% dod for example for M36- not realistic. (endless-sphere.com)
  • 4.1v 0.3C - 0.5C discharge - around 80% at 1000 cycles. (endless-sphere.com)
  • for the 90-day cumulative exposure LR = 4.44, p = 0.048, coefficient = 0.045 MN per 1000 cells per ppm-hr, 95% CI = 0.0038, 0.086). (cdc.gov)
  • Because most tumors are deficient in one or more aspects of the function of the p53 tumor suppressor, either as a consequence of mutations within p53, or impairment of upstream and downstream modulators of p53 activity 19 , targeting MK2 has the potential to selectively enhance tumor cell killing without increasing the genotoxic effects of chemotherapy on normal p53-wild type tissues. (nature.com)
  • White blood cells of the immune system that are derived from the bone marrow and spleen. (cdc.gov)
  • The effects of erythropoietin (EPO) on the behaviors of bone marrow mesenchymal stem cells (BMSCs) subjected to mechanical stretch remain unclear. (hindawi.com)
  • Using the yeast cell cycle as an example, we identified 101 cooperative module associations among 82 modules, and importantly, we established a cell cycle-specific cooperative module network. (biomedcentral.com)
  • The mutations of BRCA2 gene predispose the cells towards neoplastic development. (biomedcentral.com)
  • Notably, it promotes the osteogenic differentiation of bone mesenchymal stem cells (BMSCs) [ 15 - 17 ]. (hindawi.com)
  • Figure 4: Quantitative analysis of drug response to paclitaxel treatment at a single-cell level. (nature.com)
  • Such data are only indirectly related to quantitative changes in cells at different states of division and growth. (frontiersin.org)
  • The mechanism, which is based on the synthesis and degradation of three ''master regulator'' proteins (CtrA, GcrA, and DnaA), is converted into a quantitative model, in order to study the temporal dynamics of these and other cell cycle proteins. (ebi.ac.uk)
  • Since the method of data generation is extensible, this model can be continually modified based on new correlated, quantitative data. (ebi.ac.uk)
  • As further validation of our published findings, we have now optimized RT quantitative PCR to assess the level of viral production in cell culture, where we are finding increases in viral titer. (cdc.gov)
  • Day 2 and day 3 CM corresponding to each one of the embryos was analyzed, by quantitative PCR, for estimation of Cell-free DNA levels. (who.int)
  • Active cells secreted more extracellular polymeric substances and oxidized lignin more strongly. (lu.se)
  • CRC cells with different genetic backgrounds such as HT29, HCT116, HCT116(p53-/-), HCT116+chr3, and LoVo were treated with 5-ASA for 2-96 hours. (nih.gov)
  • Fibroblastic reticular cells (FRCs) are known to inhabit T cell-rich areas of lymphoid organs where they function to coordinate T cell and dendritic cell interactions. (harvard.edu)
  • Transforming growth factor-β [TGF-β] is such an example, being a growth stimulator in fibroblastic cells with TGF-β receptors, but a negative regulator in epithelial cells. (biomedcentral.com)
  • Individual unsynchronized cells from three different cell lines were collected in different cell cycle phases (G0/G1 - S - G2/M) with variable cell sizes. (frontiersin.org)
  • The percentages of cells in S, G 2 , and M phases in cultures from AML patients were also clearly higher than those from ALL patients. (karger.com)
  • Since many of the proteins involved in regulating the cell cycle of C. crescentus are conserved among many genera of a-proteobacteria, the proposed mechanism may be applicable to other species of importance in agriculture and medicine. (ebi.ac.uk)
  • In single-celled organisms, a single cell-division cycle is how the organism replicates itself. (wikipedia.org)
  • During EMT, cells will undergo transformation from epithelial phenotype to mesenchymal phenotype ( 14 ) and many characteristics of cells will change including loss of cell-cell adhesion and acquisition of aggressive and metastatic ability ( 15 ). (spandidos-publications.com)
  • Other TME components are investigated for the purpose of anti-stromal therapy as a method to bypass the desmoplastic reaction within certain tumour types. (lu.se)
  • Swarmer cells from wild-type C. crescentus were isolated and allowed to proceed synchronously through their 150-min cell cycle (Fig. 1A). (harvard.edu)