BindsMotilityCDC42RAC1MigrationProteinsKnockdownEpithelialPathwaysGTPaseMetastasisMetastatic cellsGenesVivoExtracellularDifferentiationOvarianApoptosisMRNAAdhesionProtein expressionProliferationRegulatesCancersAntibodyTumoursMembraneActinInvasionMonoclonalSpeciesCalponinAntibodiesBiolActivationReceptorsAcute lymphocyCytoplasmHeLaPluripotent stemTissuesMelanoma cellsCellularRolePathwayEndothelial cellTissueEffectorNodePromotesDetachmentExpressionHumanCancerousInhibitionRegulationPancreaticMicroscopyStemSynthesisKinasesProstate
Binds5
- However, despite the homology of IQGAP's GAP domain to sar1 and the fact that IQGAP1 binds Rho GTPases Rac1 and Cdc42, IQGAP does not actually have GAP function. (wikipedia.org)
- Starting at the N-terminus (or front of the protein), IQGAP1 contains a calponin homology domain (CHD), which mediates actin-binding and binds calponin. (wikipedia.org)
- IQGAP1 binds to Yes-associated protein (YAP) and modulates its transcriptional activity. (nih.gov)
- SseI binds directly to IQGAP1 and co-localizes with this factor at the cell periphery. (pasteur.fr)
- Phosphorylates IQGAP1, which binds to CDC42, mediating epithelial cell-cell detachment prior to migration. (icr.ac.uk)
Motility4
- It interacts with components of the cytoskeleton such as the formin Dia1 (mDia1), with cell adhesion molecules (CAMs), and with several signaling molecules to regulate cell morphology and motility. (wikipedia.org)
- Ito et al ( 2 ) showed that overexpression of Tβ 4 enhanced the formation of actin-based pseudopodia and cell motility in prostate cancer cells, providing direct evidence that Tβ 4 plays a role in cell migration. (spandidos-publications.com)
- Collective regulation of cell motility using an accurate density-sensing system. (bge-lab.fr)
- Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays essential roles in the regulation of multiple cellular processes linked to cytoskeletal proteins, such as cell adhesion, motility, migration and cell cycle, functions in neuron growth and ion channel regulation, and is involved in immune response, cancer cell invasion and regulation of apoptosis. (icr.ac.uk)
CDC423
- IQGAP1 does bind Rho GTPases CDC42 and RAC1, however, IQGAP1 is unusual in that it actually has no GAP function, and instead stabilizes the GTP-bound proteins in their active state. (wikipedia.org)
- By regulating filamentous actin, Cdc42 and Rac exert a profound effect on cell shape, polarity, migration, cell:cell and cell:matrix adhesion, protein traffic, and cytokinesis. (biomedcentral.com)
- In mammalian cells, as many as twenty types of protein have been reported to bind to activated Cdc42 [ 2 ]. (biomedcentral.com)
RAC11
- Colon cancer cells overexpressing Tβ 4 also exhibited enhanced cell migration, due in part to the activation of the Rac1 signaling pathway ( 3 ). (spandidos-publications.com)
Migration22
- IQGAP1 is a novel phosphatidylinositol 4,5 bisphosphate effector in regulation of directional cell migration. (nih.gov)
- Gene knockdown of Rapl expression significantly impaired LPA-stimulated migration and invasion of breast cancer cells. (uwo.ca)
- Cell viability, adhesion and migration of primary cultured RP cells were observed in the presence of Tβ4 at various concentrations ranging from 1 to 1,000 ng/ml. (spandidos-publications.com)
- Tβ4 did not affect cell viability and adhesion, but RP cell migration was stimulated by Tβ4 at concentrations of 100 and 1,000 ng/ml. (spandidos-publications.com)
- Enhancement of RP cell migration and angiogenesis is likely to be involved in the promotion of wound healing. (spandidos-publications.com)
- Upregulation of Tβ 4 by cDNA-mediated transfection was found to cause actin depolymerization in fibroblast cells ( 1 ), which demonstrated that the modulation of Tβ 4 affected the polymerization state of actin cytoskeleton, as well as other cell processes associated with the organization of actin cytoskeleton, such as cell migration. (spandidos-publications.com)
- In addition, extracellular, administered Tβ 4 promoted the cell migration of various types of cells including cardiomyocytes, human umbilical vein endothelial cells and conjunctival epithelial cells ( 4 - 6 ). (spandidos-publications.com)
- The mechanism by which exogenous Tβ 4 influences cell migration remains to be elucidated. (spandidos-publications.com)
- Besides the effect on cell migration, exogenous Tβ 4 was found to have multifunctional activities such as angiogenesis, anti-apoptosis, anti-oxidative stress and anti-inflammation ( 8 - 11 ), which emphasize its therapeutic potential in the repair of damaged tissues or wound healing. (spandidos-publications.com)
- It has roles in cell division, membrane trafficking, and cell migration. (biologists.com)
- SseI inhibits normal cell migration of primary macrophages and dendritic cells (DC) in vitro, and such inhibition requires the host factor IQ motif containing GTPase activating protein 1 (IQGAP1), an important regulator of cell migration. (pasteur.fr)
- Many cellular processes including cell division and cell migration require coordination between the actin and microtubule (MT) cytoskeletons. (umn.edu)
- In HeLa cells, contributes to hepatocyte growth factor (HGF)-induced cell migration, and in human corneal epithelial cells, plays a critical role in wound healing after activation by HGF. (icr.ac.uk)
- There's an influx of calcium into the cells that helps during this trans-migration process," Dalal said. (northwestern.edu)
- Proteomic analysis of CTGF-activated lung fibroblasts: identification of IQGAP1 as a key player in lung fibroblast migration. (musc.edu)
- The finding that activation of G12 proteins promotes cancer cell migration and invasion has highlighted the need to understand the biologic processes underlying this phenomenon. (biomedcentral.com)
- Numerous studies have identified the GTPase Rho as a major mediator of G12 signaling via its ability to effect changes in cytoskeletal dynamics required for cell migration, invasion and engagement of the cellular transcriptional machinery [ 4 , 10 ]. (biomedcentral.com)
- Our work aims for a full description of the molecular machinery that coordinates integrin traffic during cell migration as these may represent novel targets for anti-metastatic drugs. (gla.ac.uk)
- A major aim of our research is to characterise the mechanisms by which integrin-associated proteins command mRNA transport and translation during the invasive migration of cancer cells. (gla.ac.uk)
- Activation of PKC corrected problems in WAVE2 and IQGAP1 localization cell distributing and cell shape to levels observed in control cells but did not correct problems in cell migration. (conferencedequebec.org)
- Cell migration essential for malignant cell invasion and metastasis requires cross-communication between the microtubule system and actin cytoskeleton. (conferencedequebec.org)
- In addition attenuated lamellipodia protrusion was accompanied by increased fascin localization at the cell cortex and decreased cell migration velocity [17]. (conferencedequebec.org)
Proteins10
- IQGAP1 is the most well studied member of the IQGAP family of scaffold proteins. (wikipedia.org)
- We found that Rapl interacts with the multi-functional scaffolding proteins P-arrestin2 and IQGAP1 that are critical regulators breast cancer cell invasiveness. (uwo.ca)
- S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. (microarraystation.com)
- Explore our solutions for multiple applications - vaccines, monoclonal antibodies, recombinant proteins, cell or gene therapy. (vwr.com)
- Based on the analysis of differentially expressed proteins using a quantitative proteomic iTRAQ method and bioinformatics analysis, HSP90 was found to identify as a potential mediator between Raf and ERK in TNBC cells. (oncotarget.com)
- the GLK-induced exosomes transported ACE2 proteins to recipient cells, facilitating pseudovirus infection. (bvsalud.org)
- This coordination is as-yet poorly understood, but proteins such as formins and IQGAP1 are known to be involved. (umn.edu)
- Both the transforming ability and the impact on cell invasion and metastasis of G12 proteins appear to be dependent, at least in part, on their ability to engage Rho GTPases. (biomedcentral.com)
- Integrin trafficking may also guide mRNA to ribosomes associated with the protrusive regions of metastatic cells, and thus localise the synthesis of pro-migratory proteins. (gla.ac.uk)
- This download is the types and cells led from a human assembly target content soccer methylated alongside the set of two interaction localizing enzyme proteins in New Zealand. (evakoch.com)
Knockdown3
- Knockdown of IQGAP1 by specific small interfering RNA remarkably weakened invasion capacity of 2-day differentiated ovarian CSC-LCs. (bmj.com)
- Additionally, P-arrestin2 and IQGAP1 associate in response to LPA and IQGAP1 knockdown blocks breast cancer invasion. (uwo.ca)
- Factors secreted by cells expressing dominant-active Gα12 were identified by protein array, and their involvement in breast cancer cell invasion was assessed through both RNAi-mediated knockdown and antibody neutralization approaches. (biomedcentral.com)
Epithelial9
- We have found that Rapl activity can be stimulated by the bioactive lipid lysophosphatidic acid (LPA) in human breast cancer cells, but not in non-malignant mammary epithelial cells. (uwo.ca)
- Electron microscopy typically shows fusion of epithelial cell foot processes, or so-called foot process effacement. (biomedcentral.com)
- ACE2 on epithelial cells is the SARS-CoV-2 entry receptor. (bvsalud.org)
- Single-cell RNA-sequencing data derived from two COVID-19 cohorts revealed that MAP4K3/GLK-positive epithelial cells were increased in patients. (bvsalud.org)
- SARS-CoV-2-induced GLK overexpression in epithelial cells was correlated with COVID-19 severity and vesicle secretion. (bvsalud.org)
- Remarkably, SARS-CoV-2 spike protein-stimulated GLK, and GLK stabilized ACE2 in epithelial cells. (bvsalud.org)
- In epithelial cells, associates with and phosphorylates keratin-8 (KRT8), which induces targeting of desmoplakin at desmosomes and regulates cell-cell contact. (icr.ac.uk)
- D1398G Variant of MET Is Associated with Impaired Signaling of Hepatocyte Growth Factor in Alveolar Epithelial Cells and Lung Fibroblasts. (musc.edu)
- Thrombin increases lung fibroblast survival while promoting alveolar epithelial cell apoptosis via the endoplasmic reticulum stress marker, CCAAT enhancer-binding homologous protein. (musc.edu)
Pathways6
- IQGAP1 is a node intersected by many signaling pathways. (wikipedia.org)
- In addition, they play important roles in gene transcription (via activation of mitogen activated protein (MAP) kinase pathways and, in higher eukaryotes, the transcription factor NFκB), generation of reactive oxygen species, apoptosis, and cell-cycle progression. (biomedcentral.com)
- The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
- IQGAP1 can take signals from various pathways, and almost like a computer, give an output that's meaningful," Sullivan said. (northwestern.edu)
- These findings support a model where EB1 protein links microtubules to actin protrusion and cell polarity through signaling pathways including PKC. (conferencedequebec.org)
- also, the 5' download volatility trading of the cytoplasm is involved actually by cationic end-product by the RNase proximity water cell, which has a direct RNA( RNA H1 in pathways) and at least 10 Fructose-6-phosphate nucleus( activated in Jarrous 2002, Xiao et al. (evakoch.com)
GTPase5
- Ras GTPase-activating-like protein IQGAP1 (IQGAP1) also known as p195 is a ubiquitously expressed protein that in humans is encoded by the IQGAP1 gene. (wikipedia.org)
- It was hypothesized that IQGAP1 would act as a GTPase activating protein (GAP) protein, promoting the switch of ras GTPases from the active GTP to GDP-bound forms. (wikipedia.org)
- Scaffolding IQ domain GTPase-activating protein 1 (IQGAP1) plays a key role in the invasion and metastasis of cancer cells, but IQGAP1's role in cancer stem cells including ovarian cancer was unclear. (bmj.com)
- Quercetin suppresses the proliferation of multiple myeloma cells by down-regulating IQ motif-containing GTPase activating protein 1 expression and extracellular signal-regulated kinase activation. (curehunter.com)
- The small GTPase Rapl regulates cell adhesion, cell survival, gene transcription and invasion. (uwo.ca)
Metastasis4
- Cancer stem cells, as a source in cancer invasion and metastasis, possess powerful potential of differentiation. (bmj.com)
- Conclusions Increased IQGAP1 expression during the differentiation of CSC-LCs is involved in an aggressive cell behavior, which may contribute to metastasis of ovarian cancer. (bmj.com)
- Cancer stem cells (CSCs) are a subpopulation of cancer cells that have been shown to mediate metastasis and drug resistance and are associated more rapid disease progression in patients with pancreatic cancer. (grantome.com)
- These findings have provided compelling evidence that G12 signaling is an important regulator of cancer cell invasion and metastasis. (biomedcentral.com)
Metastatic cells1
- Importantly, approximately 10% of genes that show increased expression in metastatic cells are IQGAP1 binding partners. (wikipedia.org)
Genes4
- Green is ENSDARG00000078888 ( iqgap1 ) , yellow represents other genes. (ihb.ac.cn)
- An assessment of the quality of prioritization was performed using criteria, which involved an analysis of the enrichment of the top-most priority genes by genes, which are known to have simultaneous interactions with lymphedema and endothelial cell apoptosis, as well as by genes differentially expressed in murine model of lymphedema. (biomedcentral.com)
- Also, we targeted the crosstalk between IQGAPs genes with decoy receptors, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), and interleukins -8 (IL-8) and its receptor genes in a designed model of hepatocellular carcinoma induced in male Balb/c mice. (waocp.org)
- Results: Our results show that the expression of mRNA of IQGAP1, TRAIL decoy receptors, NF-κB, and IL-8 genes was elevated in hepatocellular carcinoma, as compared to normal liver tissue, while their expression was further up-regulated by increasing the dose of diethylnitrosamine. (waocp.org)
Vivo3
- Mutation of the corresponding residue in SseI (C178A) eliminates SseI function in vitro and in vivo, but not binding to IQGAP1. (pasteur.fr)
- however, the in vivo role of DUSP8 in T cells remains unclear. (bvsalud.org)
- His Tubulin research is multidisciplinary, relying on both Cell culture and In vivo. (research.com)
Extracellular4
- Raf and extracellular signal-regulated kinases (ERK) are both important therapeutic targets in the mitogen-activated protein kinase (MAPK) pathway, and play crucial roles in the apoptosis resistance of breast cancer cells. (oncotarget.com)
- Mediates cell adhesion to the extracellular matrix via integrin-dependent signaling, by mediating angiotensin-2-induced activation of integrin beta-1 (ITGB1) in cardiac fibroblasts. (icr.ac.uk)
- Exocytosis and related Cell and Extracellular. (research.com)
- In T cells, productive antigen stimulation triggers the activation of MAPK including extracellular signal-regulated kinase, p38 and JNK.33 The JNK is activated through the dual phosphorylation of its Thr and Tyr residues by mitogen-activated kinase kinase Pembrolizumab 4 (MKK4) and MKK7. (syksignaling.com)
Differentiation7
- Methods Spheroid culture with serum-free medium was used for enriching ovarian cancer stem cell-like cells (CSC-LCs) from 3AO cell line, and a medium with 10% fetal bovine serum was used to induce the differentiation of CSC-LCs. (bmj.com)
- IQGAP1 expression was decreased in ovarian CSC-LCs compared with parental 3AO cells, but increased de novo during the differentiation of CSC-LCs. (bmj.com)
- Using T cell-specific Dusp8 conditional KO (T-Dusp8 cKO) mice, mass spectrometry analysis, ChIP-Seq, and immune analysis, we found that DUSP8 interacted with Pur-α, stimulated interleukin-9 (IL-9) gene expression, and promoted Th9 differentiation. (bvsalud.org)
- Methods: We analyzed the roles of GLK and IKKß in Treg differentiation and function using T-cell-specific GLK transgenic mice and IKKß conditional knockout mice. (bvsalud.org)
- The mechanism of GLK/IKKß-mediated attenuation of Treg differentiation/function was studied by chromatin-immunoprecipitation, reporter assays, in vitro kinase assays, protein-protein interaction assays, mass spectrometry, confocal microscopy, flow cytometry, and single-cell RNA sequencing (scRNA-seq) analysis. (bvsalud.org)
- Cell Death and Differentiation , 29(10), pp. 2089-2104. (gla.ac.uk)
- HIF-1α drives the transcription of NOG to inhibit osteogenic differentiation of periodontal ligament stem cells in response to hypoxia. (stembook.org)
Ovarian1
- Expression analysis has also indicated that IQGAP1 is overexpressed in many cancers, and in more aggressive colorectal and ovarian cancers, IQGAP1 is localized at the invasive front of the neoplasm, indicating a role in mobilization of the cells. (wikipedia.org)
Apoptosis4
- In the present study, cytotoxic and apoptosis-inducing activities of the Raf/ERK dual inhibitor CY-9d were found to be restricted in triple negative breast cancer (TNBC) cells compared with ER/PR-positive cells. (oncotarget.com)
- Simultaneous treatment with the HSP90 inhibitor and CY-9d at sub-therapeutic doses was found to produce synergistic therapeutic and apoptosis-inducing effects in TNBC cells. (oncotarget.com)
- Disruption of endothelial cell apoptosis can be considered as one of the factors contributing to the development of lymphedema. (biomedcentral.com)
- Objective: We aimed to investigate the signalling crosstalk of TNF-related apoptosis-inducing ligand, TRAIL death receptors, tumour protein p53, and programmed cell death (PDCD5) with IQGAPs. (waocp.org)
MRNA7
- The mRNA and protein expression of matrix metalloproteinase 2 (MMP2) and vascular endothelial growth factor (VEGF) in Tβ4-treated RP cells was assessed by quantitative polymerase chain reaction (RT-qPCR) and western blot analysis, respectively. (spandidos-publications.com)
- Tβ4 also increased the mRNA and protein expression of MMP2 and VEGF in RP cells. (spandidos-publications.com)
- Furthermore, Il-9 mRNA levels were induced in Pur-α-deficient T cells. (bvsalud.org)
- Reduction of Il-9 mRNA levels in T cells and allergic responses of T-Dusp8-cKO mice was reversed by Pur-α knockout. (bvsalud.org)
- Consistently, scRNA-seq analyses showed that Foxp3 mRNA levels were inversely correlated with FoxO1 mRNA levels in GLK transgenic CD4+ T cells. (bvsalud.org)
- mRNA microarray analyses of A549, PC14, and PC14CDDP cells that were transiently transfected with either pre-miR-197 or LNA-miR-197 and their controls. (nih.gov)
- Treatment with VIP or PACAP prior to in vitro LC Ag presentation to CD4+ T cells enhanced IL-17A, IL-6, and IL-4 production, decreased interferon (IFN)-γ and interleukin (IL)-22 release, and increased RORγt and Gata3 mRNA expression while decreasing T-bet expression. (syksignaling.com)
Adhesion6
- IQGAP1 is a scaffold protein involved in regulating various cellular processes ranging from organization of the actin cytoskeleton, transcription, and cellular adhesion to regulating the cell cycle. (wikipedia.org)
- IQGAP1 is now recognized as a protein scaffold that integrates signals regulating cell adhesion, actin cytoskeleton, the cell cycle, and other cellular functions. (wikipedia.org)
- For example, IQGAP1 expression is necessary for neuronal process outgrowth on the cell adhesion molecule PTPmu (PTPRM). (wikipedia.org)
- Together with dynactin, dynein regulates centrosomal orientation to establish and maintain cell polarity, controls focal adhesion turnover and anchors microtubules at the leading edge. (biologists.com)
- His Cell membrane research integrates issues from Endocytosis, Secretion assay and Focal adhesion. (research.com)
- His Microtubule study integrates concerns from other disciplines, such as Focal adhesion, Exocytosis, Secretion assay, Secretion and Cell membrane. (research.com)
Protein expression2
- The quantitative real-time-polymerase chain reaction and Western blotting were performed to determine the messenger RNA and protein expression of IQGAP1, respectively. (bmj.com)
- For confirmation of gene expression, protein levels of both IQGAP1 and P53 were measured by western blot analysis, which showed that diethylnitrosamine enhanced protein expression of IQGAP1 and diminished that of p53. (waocp.org)
Proliferation2
- IQGAP1 plays an important role in the cell proliferation of multiple myeloma via the MAP kinase (ERK) pathway. (curehunter.com)
- This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. (microarraystation.com)
Regulates2
- Protein kinase C regulates human pluripotent stem cell self-renewal. (rndsystems.com)
- Thus, our data suggests a novel mechanism by which LPA regulates breast cancer cell invasiveness via Rapl, P-arrestin2, and IQGAP1. (uwo.ca)
Cancers5
- Her clinical and translational research explores whether certain immune cells called natural killer (also known as NK cells) can prevent relapse and treat high-risk cancers. (seattlechildrens.org)
- High risk human papillomaviruses (HPVs) are known to be involved in the development of cervical cancers, a number of other anogenital cancers and they are increasingly involved in oropharyngeal squamous cell carcinoma (OPSCC) [ 1 , 2 , 3 ]. (biomedcentral.com)
- cancers stem cells and will be created as therapeutic focus on to better fight cervical malignancy. (cancerdir.com)
- As an intracellular enzyme that has a detoxifying part, ALDH1A1 has been identified as malignancy stem cells marker for cervical cancers [15]. (cancerdir.com)
- In this scholarly study, we discovered that miR-23b was down-regulated in Hela and CaSki cervical cancers stem cells produced from tumorspheres. (cancerdir.com)
Antibody3
- Humanized anti-CD123 antibody facilitates NK cell antibody-dependent cell-mediated cytotoxicity (ADCC) of Hodgkin lymphoma targets via ARF6/PLD-1. (rndsystems.com)
- Description: A competitive ELISA for quantitative measurement of Human Anti centriole and centrosome antibody IgG in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (microarraystation.com)
- Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Farnesyl Diphosphate Synthase (FDPS) in Tissue homogenates, cell lysates and other biological fluids. (isogem.org)
Tumours3
- Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines and its over-expression and distinct membrane localisation is also observed in a range of tumours. (wikipedia.org)
- It responds poorly to immunotherapy for instance, despite this approach often succeeding in enlisting immune cells to fight tumours in other organs. (elifesciences.org)
- Not only do these wrap pancreatic tumours in a dense, protective layer, they also foster complex relationships with the cancerous cells: some fibroblasts may fuel tumour growth, while other may help to contain its spread. (elifesciences.org)
Membrane6
- It may play a role in cell membrane rearrangement. (microarraystation.com)
- When white blood cells arrive at blood vessel walls, the LBRC latches onto them and surrounds them with a membrane, shepherding the white blood cells through the wall and into tissue on the other side. (northwestern.edu)
- The various areas that he examines in his Cell biology study include Secretion and Cell membrane. (research.com)
- The study incorporates disciplines such as Cell growth, Glycosylation, Cell membrane, Enzyme and Glycosphingolipid in addition to Golgi apparatus. (research.com)
- loop kappa p21 D cell 4( ABCD4) is shown to reduce the CD127 performance of cobalamin( Cbl membrane parking B12) into the mitochondrion, interacting it ED for the composition of Cbl lives. (evakoch.com)
- A cytoskeleton is a complex, dynamic network of interlinking protein filaments that extends from the cell nucleus to the cell membrane. (wikipathways.org)
Actin5
- As such it has many binding partners, many of which have essential roles in control of the cell cycle and actin cytoskeleton. (wikipedia.org)
- In all systems, these GTPases are best known for their effects on the polymerization and distribution of actin in the cell cortex [ 1 ]. (biomedcentral.com)
- These observations suggest that EB1:F-actin interactions may negatively regulate EB1:MT interactions, and we speculate that this interaction may assist cells in differentially regulating MT stability in the actin-rich cortex as opposed to the cell interior. (umn.edu)
- The plus-ends of microtubules target the cell cortex to modulate actin protrusion dynamics and polarity but little is known from the molecular system that couples the interaction. (conferencedequebec.org)
- In the current studies we investigated the role of protein kinase C (PKC) in mediating EB1-dependent polarity and actin cytoskeleton remodeling in mouse melanoma cells. (conferencedequebec.org)
Invasion6
- The capacity of cell invasion was evaluated by transwell chamber assay. (bmj.com)
- In prostate cancer cells, interacts with and phosphorylates STAT3, which increases DNA-binding and transcriptional activity of STAT3 and seems to be essential for prostate cancer cell invasion. (icr.ac.uk)
- However, despite a well characterized role of Rho GTPases, the potential role of secreted factors in the capacity of G12 signaling to promote invasion of cancer cells is just beginning to be addressed. (biomedcentral.com)
- MDA-MB-231 and MCF10A breast cancer cell lines were employed as a model system to explore the involvement of secreted factors in G12-stimulated cell invasion. (biomedcentral.com)
- We found that signaling through the Gα12 in MDA-MB-231 and MCF10A breast cancer cell lines enhances expression of interleukins (IL)-6 and −8, and matrix metalloproteinase (MMP)-2, and that these secreted factors play a role in G12-stimulated cell invasion. (biomedcentral.com)
- Inhibition of IL-6 and IL-8, or MMP-2 activity significantly decreased Gα12-mediated cell invasion. (biomedcentral.com)
Monoclonal1
- Mouse monoclonal anti-EB1 antibodies (clone 5) and mouse monoclonal anti-IQGAP1 antibodies were purchased from BD Transduction Laboratories. (conferencedequebec.org)
Species1
- Homologues of IQGAP1 are known in species as divergent as yeast, worms, and humans (as well as other mammals), though the domains are not always highly conserved. (wikipedia.org)
Calponin1
- The first, the calponin homology domain, is responsible for localizing LBRC to the site along the endothelial junctions where the white blood cells are trying to cross, according to David Sullivan, PhD , research assistant professor of Pathology and lead author of the study. (northwestern.edu)
Antibodies1
- As known to all, Polyclonal Antibodies represent a heterogeneous mix of antibodies from different B cells that recognize multiple epitopes on the same antigen. (cusabio.com)
Biol2
- Am J Respir Cell Mol Biol. (musc.edu)
- Cell Mol Biol (Noisy-le-grand). (stembook.org)
Activation4
- Rationale: GLK (MAP4K3) activates PKCθ-IKKß axis in T-cell activation and induces IL-17A-mediated autoimmune diseases. (bvsalud.org)
- Phosphorylates NLRP5/MATER and may thereby modulate AKT pathway activation in cumulus cells (PubMed:19542546). (icr.ac.uk)
- Leptin and NAP2 promote mesenchymal stem cell senescence through activation of PI3K/Akt pathway in patients with systemic lupus erythematosus. (stembook.org)
- In contrast, in anergic Th1 cells, p21Cip1 persists and is available to bind to inhibit the MAPK important in early T-cell activation. (syksignaling.com)
Receptors1
- receptors and dileucine-: TLR1 photosynthesis cell in New Zealand 1945 -1960: family understood in bovine RELB of the Associations for the peptidyl of Master of Arts. (evakoch.com)
Acute lymphocy2
- To characterize gene expression signatures in acute lymphocytic leukemia (ALL) cells associated with known genotypic abnormalities in adult patients. (aacrjournals.org)
- After more than four decades of intensive research, the cellular origins of acute lymphocytic leukemia (ALL) have been well defined, and several distinct genetic mechanisms that lead to malignant transformation of these cells have been identified ( 1 - 4 ). (aacrjournals.org)
Cytoplasm1
- Remarkably, DUSP8 protein levels and the DUSP8-Pur-α interaction were indeed increased in the cytoplasm of T cells from people with asthma and patients with atopic dermatitis. (bvsalud.org)
HeLa1
- Whole genome sequencing has revealed the complexity of the HPV18 integration event in the HeLa cell line and this integration occurs within an HPV18-specific hot-spot near the c-MYC oncogene. (biomedcentral.com)
Pluripotent stem1
- Helps maintain human pluripotent stem cells in undifferentiated state. (rndsystems.com)
Tissues2
- Analysis of IQGAP1 expression in human tissues has indicated that the scaffold is more or less ubiquitously expressed. (wikipedia.org)
- Tβ 4 is distributed ubiquitously in most tissues and cells, and is also known to concentrate highly at blood platelets ( 12 ). (spandidos-publications.com)
Melanoma cells1
- 2 Materials and Methods 2.1 Cell culture and reagents B16F10 mouse melanoma cells were purchased from American Type Culture Collection (Manassas VA USA) and maintained in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (Atlanta Biologicals Lawrenceville GA USA) and antibiotics. (conferencedequebec.org)
Cellular1
- In the current study, investigators examined a protein called IQGAP1, well-known for its role in regulating a wide range of cellular processes. (northwestern.edu)
Role2
- Being able to specifically eliminate pro-cancer fibroblasts requires a better understanding of the factors that shape the role of these cells, and how to identify them. (elifesciences.org)
- High risk human papillomaviruses (HPV) plays important roles in the development of cervical cancer, a number of other anogenital cancer and they are increasingly found in oropharyngeal squamous cell carcinoma (OPSCC), however there has not been comprehensive analysis about the role how these viruses play in the development of OPSCC. (biomedcentral.com)
Pathway5
- IL-27 promotes NK cell effector functions via Maf-Nrf2 pathway during influenza infection. (seattlechildrens.org)
- Western blotting and RNA interference suggested that down-regulated IQGAP1 can attenuate the routine Raf/MEK/ERK cascade and recruit HSP90 as a bypass pathway. (oncotarget.com)
- Franck Perez focuses on Cell biology, Golgi apparatus, Endoplasmic reticulum, Secretory pathway and Microtubule. (research.com)
- His primary scientific interests are in Cell biology, Golgi apparatus, Secretory pathway, Endoplasmic reticulum and Cancer research. (research.com)
- His primary areas of investigation include Cell biology, Golgi apparatus, Endoplasmic reticulum, Secretory pathway and Microtubule. (research.com)
Endothelial cell1
- The second influential domain was the IQ motif domain, which appears to direct calcium within the endothelial cell, according to Prarthana Dalal, a sixth-year student in the Medical Scientist Training Program ( MSTP ) and second author of the study. (northwestern.edu)
Tissue4
- In other words it is found throughout the cell as well as throughout tissue types. (wikipedia.org)
- We show that Rapl expression is higher in breast cancer cell lines and tissue. (uwo.ca)
- A Northwestern Medicine study has discovered new details of a mechanism that facilitates white blood cells traveling from blood vessels into tissue, according to findings published in the Journal of Experimental Medicine . (northwestern.edu)
- William Muller, MD, PhD, professor of Pathology, was senior author of the study that discovered previously unknown details of a mechanism that lets white blood cells travel from blood vessels into tissue. (northwestern.edu)
Effector1
- We show that the bacterial effector protein SseI (also called SrfH), which is translocated into host cells by the Salmonella Pathogenicity Island 2 (SPI2) type III secretion system (T3SS), is required for Salmonella typhimurium to maintain a long-term chronic systemic infection in mice. (pasteur.fr)
Node1
- Intradermal administration of VIP or PACAP prior to application of a contact sensitizer at the injection site, followed by harvesting of draining lymph node CD4+ T cells EPZ015666 in vitro and stimulation with anti-CD3/anti-CD28 mAbs, enhanced IL-17A and IL-4 production but reduced production of IL-22 and IFN-γ. (syksignaling.com)
Promotes3
- The increased populace of malignancy stem cells is definitely one of mechanisms that promotes cervical malignancy to advanced stage. (cancerdir.com)
- CDK7-YAP-LDHD axis promotes D-lactate elimination and ferroptosis defense to support cancer stem cell-like properties. (stembook.org)
- Downregulation of RCN1 promotes pyroptosis in acute myeloid leukemia cells. (stembook.org)
Detachment1
- Trypsin/EDTA solution (Mediatech Manassas VA USA) was used for cell detachment. (conferencedequebec.org)
Expression2
- The two other members of the family include IQGAP2 and IQGAP3 which have far more restricted expression patterns in comparison with IQGAP1. (wikipedia.org)
- Identification of PARD3 signature on PARD3 deficient H157 cell line, reconstituting the expression of PARD3 gene, with a wt and a mutant form. (nih.gov)
Human3
- The present invention provides a simple and robust human liver cell-based system in which persistent hepatitis C infection, persistent hepatitis B infection or ethanol exposure induces a clinical Prognostic Liver Signature (PLS) high-risk gene signature. (sumobrain.com)
- The S100B Elisa reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (microarraystation.com)
- Description: A sandwich ELISA kit for detection of Farnesyl Diphosphate Synthase from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (isogem.org)
Cancerous1
- Malignancy stem cells (CSCs) are small population that can self-renew and differentiate into cancerous cells if necessary [12, 13]. (cancerdir.com)
Inhibition3
- DNA replication allowing time for DNA repair.30,31 p21Cip1 interaction with PCNA results in the inhibition of PCNA and thereby causes G1 and G2 block in T cells.14,32 There was some association of p21Cip1 with PCNA in stimulated control Th1 cells, but the functional significance of this low-level interaction was not determined. (syksignaling.com)
- suggests that PCNA inhibition Pexidartinib supplier by p21Cip1 is probably not the cause of proliferative unresponsiveness in these Th1 cells. (syksignaling.com)
- p21Cip1 in anergic Th1 cells instead appears to work via the inhibition of MAPK, specifically p-JNK and p-c-jun. (syksignaling.com)
Regulation2
- Present address: Department of Cell and Developmental Biology, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, 08003 Barcelona, Spain. (biologists.com)
- During cytokinesis, forms a complex with YWHAB, which is crucial for daughter cell separation, and facilitates abscission by a mechanism which may implicate the regulation of RHOA. (icr.ac.uk)
Pancreatic2
- that pancreatic cancer cells are functionally heterogeneous. (grantome.com)
- We and others identified several phenotypically distinct pancreatic cancer cell populations that are enriched in tumor-initiating capacity, defining them as cancer stem cells (CSCs). (grantome.com)
Microscopy6
- Dynamic quantitative analysis of adherent cell cultures by means of lens-free video microscopy. (bge-lab.fr)
- Lens-free video microscopy for the dynamic and quantitative analysis of adherent cell culture. (bge-lab.fr)
- Lens-free microscopy for 3D + time acquisitions of 3D cell culture. (bge-lab.fr)
- Imaging of dense cell cultures by multiwavelength lens-free video microscopy. (bge-lab.fr)
- 3D lens-free time-lapse microscopy for 3D cell culture In Advances in Microscopic Imaging , edited by Beaurepaire E, Pavone FS and So PTC, Series: Proceedings of SPIE, Spie-Int Soc Optical Engineering, 2017, vol.10414, p. (bge-lab.fr)
- Correlative live cell-immunofluorescence microscopy was performed to determine localization of WAVE2 and IQGAP1 to protruding versus retracting sides. (conferencedequebec.org)
Stem7
- strong class="kwd-title" Keywords: miR-23b, Cervical malignancy stem cells, ALDH1A1 Background Cervical malignancy is the major leading cause of cancer-related death in women worldwide. (cancerdir.com)
- Just like other CSCs, Cervical malignancy stem cells (CCSCs) are blinded to chemotherapy or radiation [14]. (cancerdir.com)
- Collectively, our data indicate that miR-23b may serve as malignancy stem cell marker form cervical malignancy and could become developed as restorative target. (cancerdir.com)
- Stem Cell Res Ther. (stembook.org)
- Nitric oxide-dependent immunosuppressive function of thymus-derived mesenchymal stromal/stem cells. (stembook.org)
- Skeletal muscle stem cells confer maturing macrophages anti-inflammatory properties through insulin-like growth factor-2. (stembook.org)
- Stem Cells Transl Med. (stembook.org)
Synthesis1
- Distinct PKC isoforms mediate cell survival and DNA synthesis in thrombin-induced myofibroblasts. (musc.edu)
Kinases1
- 3 Protein Dynamic Coupling and Allosteric Engineering of Kinases The ability to modulate protein activity in a living cell with temporal control is crucial for our understanding of biological function. (binaryoptiontradingscam.com)
Prostate1
- Withaferin A (WA) causes cell death in prostate cells. (nih.gov)