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  • Neonatal
  • In the present study, the decrease in hydrogen peroxide-induced cytotoxicity was ascertained in rat neonatal cardiomyocytes (H9C2) after the administration of A. hookeri roots extracts to determine the ameliorative effect of plants and its extracts on circulation disorders [ 5 , 6 , 7 ]. (ijpsonline.com)
  • mammalian cardiomyocytes
  • While KCC2 is absent from mammalian cardiomyocytes, understanding the role that the other KCC isoforms play in Cl − homeostasis of these cells represents a nascent area of research. (physiology.org)
  • it is generally accepted that Ca 2+ uptake via sarcoplasmic reticulum (SR) Ca 2+ -ATPase (SERCA) and Ca 2+ extrusion via sarcolemmal Na + /Ca 2+ exchanger (NCX) are main pathways for decline of cytosolic free Ca 2+ concentration ([Ca 2+ ] i ) during twitch relaxation of adult mammalian cardiomyocytes. (physiology.org)
  • Though the relative contributions of SERCA and NCX to [Ca 2+ ] i decline differ among species, it has been demonstrated that, in adult mammalian cardiomyocytes, the relative contribution of SERCA to cytosolic Ca 2+ removal is much greater than that of NCX ( 2 - 4 , 11 , 19 , 27 , 29 ). (physiology.org)
  • bone marrow
  • Introduction] Recent reports demonstrating the ability of bone marrow (BM) cells to regenerate cardiomyocytes (CMs) have prompted clinical as well as basic studies for the treatment of ischemic and non-ischemic cardiomyopathies. (ahajournals.org)
  • Background: Using a novel monoclonal antibody, CA12, we have recently immunoselected a population of highly enriched multipotential mesenchymal precursor cells (MPC) from human bone marrow. (ahajournals.org)
  • Characterization
  • Before its characterization at the molecular level, KCC was most often studied in single cells such as red cells where it was implicated in the regulation of cell volume following swelling by promoting the efflux of K + , Cl − , and osmotically obliged water. (physiology.org)
  • Our approach involves isolation of differentiated precursor subset of cells using monoclonal antibodies (mAbs) and cell sorting instruments, and subsequent characterization of their respective hematopoietic and cardiomyogenic potential in culture as well as following engraftment into mouse models of disease. (ca.gov)
  • fetal
  • Single-cell genome analyses reveal the amount of mutations a human brain cell will collect from its fetal beginnings until death. (the-scientist.com)
  • Several studies with fetal cardiomyocytes have shown the SR undeveloped and suggested that the sarcolemmal Ca 2+ influx may be a main resource of [Ca 2+ ] i transients in such cells ( 21 , 26 , 28 ), and high expression and current density of NCX were detected in embryonic cardiomyocytes ( 17 , 21 , 31 , 32 ). (physiology.org)
  • assays
  • We purified this population using three novel cell surface markers, and found a significant enrichment of cardiomyocyte clones in colony formation assays that we developed. (ca.gov)
  • sarcoplasmic reticulum
  • In mammalian adult cardiomyocytes, sarcoplasmic reticulum (SR) Ca 2+ -ATPase (SERCA) plays a major role in controlling the decline of cytosolic free Ca 2+ concentration ([Ca 2+ ] i ) in comparison with sarcolemmal Na + /Ca 2+ exchanger (NCX). (physiology.org)
  • ESCs
  • Combination of T3SS-mediated GMT delivery and Activin A treatment showed an additive effect, resulting in on average 60% of the ESCs differentiated into cardiomyocytes. (sigmaaldrich.com)
  • colony formation
  • Finally, the PRX surface with higher Mf value was found to be higher in cardiomyogenesis and beating colony formation from iPS cells, the extent of which was much higher than that on gelatin-coated surfaces. (rsc.org)
  • molecular
  • Although rapid progress is being made in many areas of molecular cardiology, issues pertaining to the origins of heart-forming cells, the mechanisms responsible for cardiogenic induction, and the pathways that regulate cardiomyocyte proliferation during embryonic and adult life remain unanswered. (ahajournals.org)
  • precursor
  • 5 Recent studies have elegantly identified several growth factors that regulate cardiomyogenic induction of the precursor cells in the anterior mesoderm. (ahajournals.org)
  • 6-9 ⇓ ⇓ ⇓ The failure of these growth factors to promote cardiomyogenic induction in more primitive precursor cells indicates that additional as of yet unidentified factors participate in the process. (ahajournals.org)
  • We discovered four precursor types from early stages of differentiating cells, each expressing genes indicative of commitment to either embryonic or extraembryonic tissues. (ca.gov)
  • therapeutic
  • Background Loss of terminally differentiated cardiomyocytes due to myocardial damage is irreversible and current therapeutic regimes are limited. (ahajournals.org)
  • In addition, we aim to develop mAbs that specifically bind to undifferentiated hESCs for removal of residual teratoma-initiating cells from therapeutic cell preparations, to ensure transplantation safety. (ca.gov)
  • proliferation
  • 13 These outer and inner curvatures play critical roles in the morphogenesis of the 4-chambered heart, as the individual chambers balloon out from the outer curvature due to the rapid proliferation of resident myocardial cells. (ahajournals.org)
  • myocardial cells
  • In contrast, myocardial cells residing in the inner curvature are thought to be relatively undifferentiated, which allows this region to participate in the alignment of the right atrium with the right ventricle and the left ventricle with the outflow tract. (ahajournals.org)
  • regulation
  • In the present study, we review approaches and studies that have shed some light on cardiomyocyte cell cycle regulation. (ahajournals.org)
  • Studies using KCC1 −/− and KCC3 −/− mice have provided strong evidence that KCC3 is the primary isoform involved in cell volume regulation following hyposmotic swelling ( 8 , 10 , 39 ). (physiology.org)
  • These findings suggest a critical role of SR in the regulation of [Ca 2+ ] i homeostasis even in differentiating cardiomyocytes. (physiology.org)
  • damage
  • Therefore, loss of cardiomyocytes causes permanent damage of heart that progressively decreases its functionality and could eventually lead to heart failure and death. (hindawi.com)