Cells in vivo cell tracking. Medical search. Frequent questions

Detection thresholds of the labeled cells in vitro and in vivo were determined to be 10 4 and 10 5 cells, respectively. (biomedcentral.com)
Using high-throughput drug screening, he found that benztropine, an approved drug for Parkinson's disease, had the attributes he sought, and resulted in some of the in vitro and in vivo remylenation he was looking for. (ca.gov)
To achieve our objectives, we use start of the art next-generation sequencing technologies, in vitro and in vivo models of acute leukemia, cell sorting, single-cell sequencing and advanced bioinformatics. (lu.se)

Ex vivo MPI and fluorescence microscopy confirmed that pLN MPI signal was due to originally injected Ad SPIO+ and SPIO+ DC. (bvsalud.org)
In all three rats subjected to SCI, green fluorescence was shown to spread from the site of BMSC-GFP injection toward the injury site, suggesting that the transplanted cells had migrated toward the lesion within the 4-week post-transplantation period. (korea.ac.kr)
Histological evaluation suggested that the detected green fluorescence was emitted by cells that had distributed in the dorsal white matter, and demonstrated that some of the transplanted cells expressed neuronal or astrocytic markers. (korea.ac.kr)
Here, we review and discuss the advantages and limitations of a number of strategies commonly used to label and track cells at high resolution in vivo in mouse and zebrafish, using fluorescence microscopy. (ox.ac.uk)
In wild-type and mutant tissues, the authors measured ionic and pH responses to flg22, as well as PIP2;1 localization in guard cells and subsidiary cells using variable-angle total internal reflection fluorescence microscopy (VA-TIRFM), which allowed them to follow the movements of single particles. (plantae.org)
Therefore, we use 4d in vivo fluorescence microscopy data, which offers a variety of mathematical challenges. (helsinki.fi)
After a droplet is introduced between cells in a tissue," the authors continued, "local stresses are determined from droplet shape deformations, measured using fluorescence microscopy and computerized image analysis. (genengnews.com)

This study aims to explore methodology and to understand in vivo biodistribution of clinical-grade haMSCs labeled with fluorescent dye and injected into an immunocompetent OA rat model. (biomedcentral.com)
There remains an urgent need for the noninvasive tracking of transfused chimeric antigen receptor (CAR) T cells to determine their biodistribution, viability, expansion, and antitumor functionality. (snmjournals.org)
However, the fate of MSCs in vivo including their overall biodistribution and local tissue quantities is not fully known, especially how these parameters change over time. (iwmpi.org)
Radiolabeling efficiency under various conditions, in vitro stability in serum at 37 °C, and in vivo biodistribution and imaging in normal mice over 48 h were studied. (acs.org)
In vivo biodistribution profiles in normal female Balb/C mice were similar for all the macrocyclic radioimmunoconjugates with most of the activity remaining in the blood pool up to 48 h. (acs.org)

Here, we report that in AD patients and two mouse models of AD, overexpression of serum response factor (SRF) and myocardin (MYOCD) in cerebral vascular smooth muscle cells (VSMCs) generates an Aβ non-clearing VSMC phenotype through transactivation of sterol regulatory element binding protein-2, which downregulates low density lipoprotein receptor-related protein-1, a key Aβ clearance receptor. (nature.com)
Researchers from Kings College London and University College London have been awarded £500,000 from Cancer Research UK's Multidisciplinary Project Award, to develop a novel PET imaging reagent for Chimeric Antigen Receptor (CAR) T-cell in vivo tracking. (kcl.ac.uk)
In this article, the authors presented "a method to quantify cell-generated mechanical stresses exerted locally within living embryonic tissues, using fluorescent, cell-sized oil microdroplets with defined mechanical properties and coated with adhesion receptor ligands. (genengnews.com)
TARC, a CC chemokine, is predominantly produced by dendritic cells in the thymus, and signals through the CCR4 receptor. (peprotech.com)
Chimeric antigen receptor T cells (CARTs) are a novel cell-based therapy designed to direct host-derived T-cells against cancer cells through tumor-specific surface receptors. (medpagetoday.com)
Chimeric antigen receptor (CAR) T-cell therapy can induce durable remissions and possibly cure patients with relapsed/refractory diffuse large B-cell lymphoma (DLBCL) with poor prognosis and no standard therapeutic options. (medpagetoday.com)
Subsequently, bioactivity assays were evaluated in vitro using the TF-1 tyrosine receptor kinase A (Trk-A) expressing cell line. (frontiersin.org)
Investigators at the Department of Clinical Genetics have a strong track record in identifying novel cell surface markers on leukemia stem cells and were the first to demonstrate the potential therapeutic use of recombinant antibodies directed against the marker IL1RAP (interleukin-1 receptor accessory protein) on cancer stem cells in chronic myeloid leukemia (CML) and acute myeloid leukemia (AML). (lu.se)
This inhibits cleavage of the sialyl-acid bond to the host receptor, thus the virus is unable to be released from infected host cells and spread to new cells. (who.int)

The distribution and differentiation of the transplanted cells were subsequently evaluated with immunohistochemistry. (korea.ac.kr)
Senescent cells supply beneficial growth factors which promote myofibroblast differentiation. (jax.org)
Based on these findings, the Campisi team proposes that PDGF-AA derived from senescent cells stimulates local myofibroblast differentiation, allowing for greater contractility and more efficient wound closure. (jax.org)
The proliferation and differentiation of pluripotent stem cells give rise to progeny that can populate the entire immunologic and hematopoietic systems through committed progenitors of both the lymphoid and myeloid lineages. (medscape.com)
Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)

Such noninvasive bioimaging techniques would be valuable for monitoring the fate of these transplanted cells and assessing the safety and efficacy of their transplantation. (korea.ac.kr)
Over the years, several dyes have been developed to allow rapid, effective and bright ex vivo labelling of cells for subsequent transplantation and imaging. (ox.ac.uk)
In this ongoing Phase II multicenter study, 55 heavily pretreated patients with relapsed/refractory DLBCL, including almost 50% without a response to autologous stem cell transplantation (ASCT), were treated with fludarabine/cyclophosphamide lymphodepletion, followed by a dose of JCAR17. (medpagetoday.com)
A more appealing strategy involves the transplantation of beta cells grown from stem cells, particularly with recent advances in generating large numbers of beta cells from human stem cells. (medscape.com)

â ¢ Magnetic particle imaging is a suitable modality to quantify in vivo dendritic cell migration. (bvsalud.org)
Such changes in dendritic cell migration may be associated with immunological events that maintain inflammation at the sites of infection. (biomedcentral.com)

Imaging studies showed 86 Y-AABD was retained by DAbR1-positive T cells while it continuously cleared from normal tissues, allowing for in vivo tracking of intravenously administered CAR T cells. (snmjournals.org)
Technological advances that allow deeper penetration in live tissues, such as the development of confocal and two-photon microscopy, together with the generation of ever-new fluorophores that facilitate bright labelling of cells and tissue components have made imaging of vertebrate model organisms efficient and highly informative. (ox.ac.uk)
Senescent cells in aging tissues are characterized by increased inflammatory cytokine and growth factor secretion. (jax.org)
Mechanochemical Principles of Spatial and Temporal Patterns in Cells and Tissues. (mpi-cbg.de)
Patterns are ubiquitous in living systems and underlie the dynamic organization of cells, tissues, and embryos. (mpi-cbg.de)
Although the tractive forces exerted by cells roaming petri dishes have been measured, hardly anything is known about the forces generated by cells as they assemble three-dimensional tissues and shape embryonic organs. (genengnews.com)
The technique was described in an article published December 8 in Nature Methods, in an article entitled "Quantifying cell-generated mechanical forces within living embryonic tissues. (genengnews.com)
Highlighting the differences between cellular forces generated in an embryo and those generated in a petri dish, Dr. Ingber, director of the Wyss Institute, added, "It has not been possible to demonstrate a direct causal relationship between mechanics and behavior in vivo because we previously had no way to directly quantify force levels at specific locations in 3D living tissues. (genengnews.com)
The technique," they wrote, "is well suited for any study that requires quantification of stresses generated by individual living cells or groups of cells in culture, embryonic tissues or adult organs. (genengnews.com)
Organ-on-chips refer to the cell culture devices based on microfluidic technology to simulate the physiological functions and behaviors of tissues and organs through arranging living cells in a micrometer-sized chamber and the continuous supply of cell culture media. (selectbiosciences.com)
There is also an imaging station for imaging cells, tissues and small animals with simultaneous monitoring of vital functions and motion tracking. (lu.se)
Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)

In 2011, we showed that a stem cell population (named MuStem cells) isolated from healthy dog skeletal muscle induces long-term muscle repair and striking clinical efficacy after its systemic delivery in the dystrophic dog representing the clinically relevant DMD animal model. (emc-proceedings.com)
There are currently over 800 registered clinical trials that use mesenchymal stem cells (MSCs) for tissue repair and immunomodulation. (iwmpi.org)
Multi-modal imaging techniques that can track cell therapeutics may allow facilitation and optimization of clinical translation therapeutic outcome. (iwmpi.org)
In preclinical and clinical studies, progenitor cell therapy (cord blood and mesenchymal stem cells) has shown promise in reversing the underlying pathology of SNHL, the loss of cochlear sensory hair cells. (intechopen.com)
We will present a summary of the effect of hearing loss on auditory development, existing preclinical and clinical data on progenitor cell therapy, and its potential role in the (re)habilitation of non-genetic SNHL. (intechopen.com)
These models are more predictive of clinical outcomes and are superior to cell lines for preclinical drug evaluation and therapeutic strategies. (biorxiv.org)
Patient-derived xenografts, or PDXs, have begun to supplant traditional cell lines due to their retention of clinical biomarkers and heterogeneity from the original tumour ( 10 ). (biorxiv.org)
Pneumonia virus of mice (PVM) is a natural rodent pathogen that replicates in bronchial epithelial cells and reproduces many clinical and pathological features of the more severe forms of disease associated with human respiratory syncytial virus. (nih.gov)
One fundamental challenge in the successful development and clinical application of CAR T-cell based therapies, is the need to better understand their behaviour and location once they are injected into patients. (kcl.ac.uk)
Integrated imaging studies are essential to visualise the CAR T-cells in patients at the earliest stage of preclinical and clinical development of cell based treatments. (kcl.ac.uk)
I am an MRC Clinician Scientist and Honorary Consultant Paediatric Oncologist, providing a clinical service for young people with lymphoma or Langerhans cell histiocytosis at the Great North Children's Hospital, Newcastle. (ncl.ac.uk)
I also work closely with UK and European colleagues (iBFM, EICNHL, ESID) to develop our understanding of development of lymphoid malignancy in children with inherited immune deficiency or DNA repair disorders and provide the paediatric clinical service for Langerhans cell histiocytosis and collaborate with Newcastle Human Dendritic Cell Laboratory ( http://www.hudendritic.org/ ) run by Professor Matt Collin. (ncl.ac.uk)
By generating recombinant antibodies against a set of such markers, validating their clinical use, and providing in vivo proof-of-concept data in preclinical models for a targeted antibody-based therapy, novel diagnostic and therapeutic modalities for AML will be designed. (lu.se)
The biological properties and clinical potential of stem cells elicit that are generated must not be unduly sensitive to small fluctu- continued scientific, commercial, and public interest. (lu.se)
[ 3 ] Clinical trials are currently under way on the safety, tolerability, and efficacy of transplanting such cells in encapsulated forms in persons with type 1 diabetes, with results to be announced in the coming years. (medscape.com)

However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)
To enable the tracking of Arc molecules from individual neurons in vivo, we devised an adeno-associated virus (AAV) mediated approach to tag the N-terminal of the mouse Arc protein with a fluorescent reporter using CRISPR/Cas9 homologous. (lu.se)
To enable the tracking of Arc molecules from individual neurons in vivo, we devised an adeno-associated virus (AAV) mediated approach to tag the N-terminal of the mouse Arc protein with a fluorescent reporter using CRISPR/Cas9 homologous independent targeted integration (HITI). (lu.se)
Huntington disease (HD) is an incurable, adult-onset, autosomal dominant inherited disorder associated with cell loss within a specific subset of neurons in the basal ganglia and cortex. (medscape.com)

However, understanding the mode of action of the cell therapy has been limited by cell tracking capability and correlation between the pharmacokinetics of the injected cells and the intended pharmacodynamics effect. (biomedcentral.com)
The current study represents the first attempt to correlate cell therapy efficacy on OA with the physical presence of the injected haMSCs in the OA model, and demonstrates that human adipose-derived mesenchymal stem cells persisted for 10 weeks locally in the rat joint, coinciding with the efficacy observed. (biomedcentral.com)
However, the success of CAR T-cell therapy for solid tumors has been limited so far, and the mechanisms for the tumor's treatment resistance are not well understood. (snmjournals.org)
Progenitor cell therapy may also allow functional reorganization of the auditory pathways including primary auditory cortex (Heschl's gyrus). (intechopen.com)
To establish a platform where an international network of experts from multiple sectors (public and private sector research scientists, regulators, clinicians, health foundations, and technology developers) can collaborate sharing knowledge, experience, and resources in the rapidly evolving field of cell therapy. (hesiglobal.org)
The HESI CT-TRACS Tumorigenicity Working Group will leverage its successful track record of multi-laboratory evaluation of relevant methods and assays in support of tumorigenicity evaluation of cell therapy products in support of the challenge winners. (hesiglobal.org)
Some of the recent focuses include salt nanoparticle therapeutics, nanoparticle-based adjuvants for enhancing immunotherapy, nanoparticle-based radiosensitizers, X-ray induced photodynamic therapy or radiodynamic therapy, and cell-based drug delivery. (uga.edu)
Our aim is to deeply characterise paediatric B-cell non-Hodgkin lymphoma to provide a comprehensive understanding of disease initiation and evolution in response to therapy. (ncl.ac.uk)
These cells are not killed by conventional cancer therapy and thus it is critical to identify those cancer stem cells to be able to improved diagnostics and treatment of cancer. (lu.se)
In type 1 diabetes , beta cells succumb to autoimmunity, leaving individuals with significantly reduced beta-cell mass and a lifelong dependence on insulin replacement therapy. (medscape.com)

No significant differences in viability, phenotype and in vivo pLN migration were noted for Ad SPIO+ and SPIO+ DC. (bvsalud.org)
In work performed by Division of Translational Cancer Research it is shows that the hypoxic state pushes neuroblastoma and breast cancer towards an immature, stem cell-like phenotype. (lu.se)

The successful candidate will join a team of researchers who investigate new CAR T-cell therapies for the treatment of glioblastoma in mouse models with multi-modality imaging techniques. (stanford.edu)
Imaging of PDX cells provides a powerful system to better develop personalized therapies targeting breast cancer brain metastasis. (biorxiv.org)
While these cell lines have been well characterized, they do not represent the tumour heterogeneity, metastatic behaviours, and histopathology seen clinically, and are unsuitable for evaluating therapies due to their fast progression in vivo ( 8 , 9 ). (biorxiv.org)
The T-ALERT Challenge aims to develop an in vitro assay that can reliably evaluate tumourigenicity of human-engineered T cell therapies with the potential to be applied to multiple immune cell types. (hesiglobal.org)
This working group aims to identify current approaches, gaps, and needs in monitoring/evaluating the fate and activity of cells after their administration in vivo , to assess the safety of cell-based therapies. (hesiglobal.org)
The third project is focused on identifying targets affecting leukemia stem cells and on providing in vivo proof of concept data for targeted therapies with a focus on antibody-based strategies. (lu.se)
Although a few FDA-approved therapies for type 2 diabetes (meglitinides, sulfonylureas, incretin-based drugs) directly enhance beta-cell function, no therapies for any form of diabetes lead to the growth of healthy, new beta cells to replace those that were lost or dysfunctional. (medscape.com)

Dr. Robert Kass (Columbia University), gave further examples of the power of pluripotent stem cells to ameliorate a bad situation. (ca.gov)
This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)
These strat- egies provide a robust way to visualize and isolate specific cell pop- ulations of differentiated pluripotent stem cells. (lu.se)
In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)

To study PDX cells preclinically, we used both bioluminescence imaging (BLI) to evaluate cell viability and magnetic particle imaging (MPI), an emerging imaging technology to allow for detection and quantification of iron nanoparticles. (biorxiv.org)

Despite widespread study of dendritic cell (DC)-based cancer immunotherapies , the in vivo postinjection fate of DC remains largely unknown. (bvsalud.org)
RELEVANCE STATEMENT Tracking the in vivo fate of DC using noninvasive quantifiable magnetic particle imaging can potentially serve as a surrogate marker of therapeutic effectiveness . (bvsalud.org)
Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)
In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)
2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)

Although MSC is not a traditional drug, and preclinical data generated from MSC may not always be as instructive as for drug molecules, investigating the PK/PD of MSC is essential to understand the dosing, scheduling, and mode of action (MOA) in vivo. (biomedcentral.com)
The presence of these labeled cells coincided with wound-healing markers, such as p16, p21, SA-β-gal, and inflammatory and pro-angiogenic molecules. (jax.org)
In addition, validation of preliminary findings of solute carrier molecules (usually used for drug-delivery) selectively expressed in tumor cells with the aim to develop imaging-led theranostic approaches of personalized treatment will be performed. (lu.se)
Our facilities provide the opportunity to study molecules, cells, organs and entire organisms. (lu.se)

Labeled cells were used to establish detection threshold of a noninvasive biofluorescent imaging system before the cells (2.5 × 10 6 ) were injected into a conventional rat OA model induced by medial meniscectomy for 8 weeks. (biomedcentral.com)

PhD, MD/PhD or equivalent with a major in biomaterial design, cell and molecular biology, biochemistry or Regenerative Medicine. (stanford.edu)

Magnetic particle imaging (MPI) has emerged as a suitable modality to quantify in vivo migration of superparamagnetic iron oxide (SPIO)-labeled DC. (bvsalud.org)
Herein, we describe a popliteal lymph node (pLN)-focused MPI scan to quantify DC in vivo migration accurately and consistently. (bvsalud.org)
We overcame a reported limitation of MPI by using a pLN-focused MPI scan to quantify pLN-migrated Ad SPIO+ and SPIO+ DC in 100% of cases and detected as few as 1000 DC (4.4 ng Fe) in vivo. (bvsalud.org)
Here, the utility of OX40, a costimulatory molecule mainly expressed on activated effector T cells known to play an important role in eliminating cancer cells, was evaluated as a PET imaging biomarker to quantify and track response to immunotherapy. (aacrjournals.org)
The paper's authors reported that after applying their oil-drop method, they were able to quantify the anisotropic stresses generated by mammary epithelial cells cultured within 3D aggregates. (genengnews.com)

Two days later, in vivo DC migration was quantified using whole animal , pLN-focused, and ex vivo pLN MPI scans. (bvsalud.org)
KEY POINTS â ¢ Adenoviral-transduced and iron oxide -labeled dendritic cells are in vivo migration competent. (bvsalud.org)

Determined by its ability to chemoattract murine BW5147.3 thymic lymphoma cells. (peprotech.com)
Finally, in vivo studies using our well-established murine tibia fracture model were performed and a single injection of the β-NGF loaded PEGDMA microrods, non-loaded PEGDMA microrods, or soluble β-NGF was administered to assess the extent of fracture healing using Micro-computed tomography (µCT) and histomorphometry. (frontiersin.org)
In vivo studies using our murine tibia fracture model show that PEGDMA microrods injected at the site of fracture remained adjacent to the callus for over 7 days. (frontiersin.org)
This article compares the cytotoxicity and cellular interactions of positively and negatively charged CdSe/CdS/ZnS QDs prepared by a microwave method using a murine alveolar macrophage-like cell culture model. (cdc.gov)

Recent experimental studies have shown that bone marrow stromal cells (BMSC) differentiate into neural cells and reduce neurological deficits when transplanted into traumatized spinal cord. (korea.ac.kr)
We conducted a study directed chiefly at developing a non-invasive system for tracking BMSC transplanted into the spinal cord of living animals. (korea.ac.kr)
These results suggest the possibility of tracking BMSC transplanted into the spinal cord in living animals. (korea.ac.kr)
9. Björklund, A., Skagerberg, G.: Evidence for a major spinal cord projection from the diencephalic A11 dopamine cell group in the rat. (lu.se)

Human adipose-derived mesenchymal stem cells (haMSCs) have shown efficacy in treating osteoarthritis (OA) both preclinically and clinically via intra-articular (IA) injection. (biomedcentral.com)
We attempted to reveal the existence of labeled cells in vivo by imaging and a molecular biomarker approach, and to correlate with the in vivo efficacy and physical presence over a follow-up period up to 10 weeks. (biomedcentral.com)
10 5 cells) lasted for about 10 weeks in the surgical knee joint at the same time as efficacy was observed. (biomedcentral.com)
Current methods used to predict or monitor the activity of infused T cells in patients provide limited data on treatment efficacy ( 6 , 7 ). (snmjournals.org)
Currently, little is known about the homing in the muscle tissue and the whole body distribution of the MuStem cells following a systemic delivery which are nevertheless essential to determine the safety and efficacy aspect of the therapeutic strategy. (emc-proceedings.com)
Most recently, data for the efficacy of the JCAR17 platform for relapsed/refractory diffuse large B-cell lymphoma (DLBCL), the most common lymphoma in the United States, were presented by Dr. Jeremy Abramson and his colleagues at the 2017 International Conference on Malignant Lymphoma. (medpagetoday.com)

When senescent cells were killed by GCV treatment, wound closure was delayed, and immune and endothelial cells were absent. (jax.org)
The etiology of bone marrow failure (BMF) includes defective stem/progenitor cells and/or stroma/accessory cells/growth factors, as well as deficient nonspecific nutrients or, as in the case of acquired aplastic anemia, immune-mediated abnormalities. (medscape.com)
When bacteria like salmonella infect and sicken people, they hijack a person's cell proteins to develop a defense against an immune response. (purdue.edu)
Salmonella bacteria fend off a cell's immune defenses by creating a pocket within the cell, called a Salmonella-containing vacuole, in which to hide. (purdue.edu)
CAR T-cell immunotherapy is a new type of cancer treatment in which a patient's T-cells (a type of immune or white blood cell) are modified in the laboratory so they will bind to the cancer cells and kill them. (kcl.ac.uk)
Spatiotemporal in vivo tracking of polyclonal human regulatory T cells (Tregs) reveals a role for innate immune cells in Treg transplant recruitment. (peprotech.com)

The major breakthrough is the identification of a biomarker of HIV persistent cells in vivo. (europa.eu)

Quantitative "hot-spot" imaging of transplanted stem cells using superparamagnetic tracers and magnetic particle imaging. (iwmpi.org)

Successful treatment of B-cell malignancies with CD19-CAR T cells was a milestone for this approach ( 4 , 5 ). (snmjournals.org)
Still missing from these approaches is any attempt to induce regeneration or replication of endogenous beta cells in the pancreas, an approach that would be physiologically desirable. (medscape.com)

DAbR1 was subcloned together with green fluorescent protein into an SFG-retroviral vector and used to transduce CD3/CD28-activated primary human T cells and second-generation 1928z (CAR) T cells. (snmjournals.org)
We have developed a fully functional recombinant PVM strain with a fluorescent reporter protein (rK2-PVM) that permits us to track infection of target cells in vivo. (nih.gov)
Protein Nanocage Mediated Fibroblast-Activation Protein Targeted Photoimmunotherapy To Enhance Cytotoxic T Cell Infiltration and Tumor Control. (uga.edu)
Bioactivity of protein post-loading was confirmed with the TF-1 cell line. (frontiersin.org)
When inducing long-term potentiation (LTP) in the hippocampus, we observed an increase of Arc protein highly correlated with an increase in fluorescent intensity and the number of mCherry-positive cells. (lu.se)
In addition to infrastructures for bioimaging, protein and genes & cells, we also provide other resources e.g., databases, networks and specialized labs. (lu.se)

Our preliminary experience with CARTs has been primarily against a variety of relapsed, refractory B-cell malignancies including ALL, CLL, and NHL. (medpagetoday.com)
Nevertheless, as the CART repertoire expands to other B-cell malignancies and solid cancers, we can expect to see further studies investigating potential variables regarding their function in various settings. (medpagetoday.com)

Experience in stem cell and islet biology is highly desirable. (stanford.edu)

Using these p16-3MR mice and in vivo bioluminescent imaging, the researchers observed a robust, but transient, increase in senescent cells at the site of cutaneous injury. (jax.org)
Likewise, p16/p21 double knockout mice, which have very few senescent cells, showed similar delays in wound closure. (jax.org)
Scar tissue (stained for collagen, in orange) is evident in healed wounds from transgenic p16-3MR mice lacking senescent cells (bottom panel, GCV-treated) compared to control mice (top panel, PBS-treated). (jax.org)
To understand how senescent cells promote repair, the Campisi team surveyed wound sites in GCV-treated p16-3MR mice and found fewer endothelial cells and fibroblasts, including myofibroblasts, which are contractile cells responsible for wound closure. (jax.org)
NOD/SCID/ILIIrg -/- (n=5) mice received injections of 1×10 6 iron-labeled F2-7 cells into the fourth mammary fat pad (MFP). (biorxiv.org)
CD11c(+) sialic acid-binding immunoglobulin-like lectin F(+)) in vivo and likewise detect mKATE2(+) DCs in mediastinal lymph nodes from infected mice. (nih.gov)
In contrast, AMs from L. plantarum-primed mice challenged with virus ex vivo exhibited no differential susceptibility to rK2-PVM. (nih.gov)
Uninfected or Leishmania -infected thioglycollate-elicited peritoneal exudate cells were transferred from C57BL/6 to BALB/c mice or from Ly5.1 + to Ly5.1 - mice. (biomedcentral.com)
[ 7 ] More recent data from striatal neuronal cultures transfected with mutant huntingtin and transgenic mice carrying the spinocerebellar ataxia-1 (SCA-1) gene (another CAG repeat disorder) suggest that NIIs may not be necessary or sufficient to cause neuronal cell death, but translocation into the nucleus is sufficient to cause neuronal cell death. (medscape.com)
This team showed that injection of just three genes- Neurog3, Mafa, and Pdx1 -into the pancreatic parenchyma of mice leads to conversion of exocrine cells to functional beta cells, a process popularly known as "reprogramming. (medscape.com)

Accelerated hematopoietic cell apoptosis has been demonstrated in virtually all IBMFS. (medscape.com)

These cells include dermal resident dendritic cells (DC), epidermal Langerhans cells, neutrophils and monocytes, which are released from the blood when the dermal capillary is disrupted and a reputed blood lake is formed [ 3 - 6 ]. (biomedcentral.com)
DR+ epidermal macrophage induction, and noma in young women: An evaluation of the Langerhans cell depletion. (who.int)

In Vivo Micro-CT imaging of human mesenchymal stem cells labeled with gold-poly-L-lysine nanocomplexes. (iwmpi.org)

Understanding how that works and developing methods for defending against these bacteria is difficult because scientists haven't been able to track the hundreds of proteins involved in real time. (purdue.edu)
The "In vivo Brain Circuit Analysis" platform is a research infrastructure devoted to integrating methods and techniques to interrogate neuronal circuits in health and disease using advanced methods for the perturbation and recording of cell activity. (lu.se)

Cell Biol. (nature.com)
Cell Chem Biol. (nih.gov)
Nat Rev Mol Cell Biol , Art. (mpi-cbg.de)

This is the first study to provide support for inter-neuronal in vivo transfer of Arc in the mammalian brain. (lu.se)

The project will provide important information about whether the therapeutic cells are safe to use, how they kill the cancer cells, and how effective the CAR T-cell based treatments are. (kcl.ac.uk)
The strategy used to identify new therapeutic markers on leukemia stem cells will also be explored in other tumor types studied within the MAD for Cancer Program. (lu.se)

2000: Two papers in Nature report synthetic biological circuits, a genetic toggle switch and a biological clock, by combining genes within E. coli cells. (wikipedia.org)
The precise pathophysiology of inherited single cell and multilineage cytopenias has not been elucidated despite the identification of many of the genes mutated in these disorders. (medscape.com)
explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)
Below is a non-exhaustive list of in-house infrastructures that are categorized into three overarching themes: bio-imaging, proteins, genes & cells and other resources. (lu.se)
Below you can see some examples of the infrastructure for research on genes and cells, available for researchers at Lund University. (lu.se)
For example, how can genes be delivered at the precise dose to cells in humans? (medscape.com)

Besides promising to explicate the role of cell-generated mechanical forces in embryonic development, this technique may advance knowledge regarding other processes including birth defects, tumor growth and metastasis, and diseases in which imbalanced cellular forces play a role. (genengnews.com)
In addition, they confirmed that these stresses "are dependent on myosin II activity and are more than twofold larger than stresses generated by cells of embryonic tooth mesenchyme, either within cultured aggregates or in developing whole mouse mandibles. (genengnews.com)
We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)

Hematopoietic cells are almost totally absent. (medscape.com)
The microenvironment, consisting of lymphocytes, macrophages, fibroblasts, endothelial elements, and stroma in which hematopoietic cells reside, creates a regulatory niche that determines the local area network. (medscape.com)

Presentations documented the impact of stem cell research on the understanding of human biological mechanisms, amelioration of human health, and treatment of disease and trauma. (ca.gov)

This specific construct consists of equal quantities of CD4+/CD8+ CARTs which are transduced separately after apheresis and directed against CD-19 on tumor cells subsequently in a 1:1 infusion. (medpagetoday.com)
1995) and subsequently in primary cells (Heyworth et al. (lu.se)
Using careful, state-of-the-art cell tracking techniques, the same group subsequently showed that Pax4 misexpression initially caused the conversion of cells lining the ducts to become alpha cells, which were then converting to beta cells. (medscape.com)

2006: Researchers engineer a synthetic circuit that promotes bacterial invasion of tumour cells. (wikipedia.org)
The Scientific Symposium at Stem Cell Meeting on the Mesa (SCMOM) aims to advance scientific progress through the collaboration of over 16 researchers and academic leaders who were selected to present at the 8th annual conference in 2013. (ca.gov)
The iPS cells from the patient allowed the doctors and researchers to understand the specific genetic background of this patient that caused the bad, off-target effects of the drugs usually used to treat Long QT Syndrome. (ca.gov)

Genetic manipulation leading to expression of fluorescent proteins is undoubtedly the labelling method of choice and has been used to visualize several cell types in vivo. (ox.ac.uk)
Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)

Insight into the mechanisms and pathophysiology of breast cancer brain metastasis have long relied on the use of immortalized cell lines that have been injected intracardially into the left ventricle of the mouse heart in order to selectively grow tumours within the brain. (biorxiv.org)
We still know very little about the mechanisms controlling the ability of these cells containing live parasite or parasite fragments to remain in the infection site or to migrate to the draining lymph node. (biomedcentral.com)
The precise B cell subsets, mechanisms, and factors that facilitate the maintenance of latency and prevent reactivation are not fully understood. (nature.com)

The goal of this study was to develop the first successful iron labeling method of breast cancer cells derived from patient brain metastases and validate this method with imaging during tumour development. (biorxiv.org)
Biocompatible and label-free separation of cancer cells from cell culture lines from white blood cells in ferrofluids. (uga.edu)
immunotherapeutics being a prime example of the latter) with the potential to eliminate all cancer cells, despite their clonal heterogeneity and their surrounding microenvironment. (nih.gov)

Neuraminidase inhibitors block the release of progeny virions from a host cell by selectively binding to the active site of the neuraminidase enzyme. (who.int)
To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)

In type 2 diabetes , beta cells fail to secrete sufficient insulin to overcome the prevailing insulin resistance . (medscape.com)

Chimeric antigen receptors (CARs) endow T cells with antigen-specific recognition, activation, and proliferation independent of major histocompatibility complex ( 1 - 3 ). (snmjournals.org)

Selective T cell ablation in vivo with 177 Lu-AABD seems feasible for clustered T-cell populations. (snmjournals.org)
To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)

In order to track virus-target cell interactions during acute infection in vivo, we developed rK2-PVM, bacterial artificial chromosome-based recombinant PVM strain J3666 that incorporates the fluorescent tag monomeric Katushka 2 (mKATE2). (nih.gov)
With rK2-PVM, we demonstrate infection of leukocytes in the lung, notably, dendritic cells and alveolar macrophages. (nih.gov)
We show here that Lactobacillus administration also limits infection of leukocytes in vivo and results in diminished release of infectious virions from alveolar macrophages. (nih.gov)
We predict that ABCs could be playing a role throughout γHV68 infection due to their long-term persistence, activation of T cells, and continuous cytokine and antibody production. (nature.com)
B cells are also known to be important during latent γHV68 infection. (nature.com)

In addition, they discovered that senescent cells fluorescently isolated from p16-3MR wounds produced large amounts of PDGF-AA, a mitogen and chemoattractant for fibroblasts. (jax.org)

The preclinical instrument is stationed in the in vivo department of Lund University Biomedical Center (BMC), and is managed by Associate Professor Gustav Smith. (lu.se)

During last years, our group isolated the human counterparts (hMuStem) and demonstrated that they share the same phenotypic and in vitro behavioral features of canine cells. (emc-proceedings.com)
Accumulating data suggests that many human tumors are organized in cellular hierarchies initiated and maintained by a small population of self-renewing so called cancer stem cells. (lu.se)
Although possible, these strategies are often complicated to transfer to human cells due to technical issues (9 ), and only a few successful cases have been described ( 10, 11 ). (lu.se)
Here we describe how this system can be used for human embry- onic stem cells. (lu.se)

Cells with high expression of HIF-2 α have properties rendering the tumors aggressive behavior potentially being the neuroblastoma tumor stem cells. (lu.se)

Anatomy1

Analytical, Diagnostic and Therapeutic Techniques and Equipment2

Phenomena and Processes8

Vitro and in vivo3

Fluorescence7

Biodistribution5

Receptor9

Differentiation9

Transplantation4

Dendritic cell migration2

Tissues12

Clinical15

Neurons5

Therapy10

Phenotype2

Therapies7

Pluripotent5

Viability1

Fate5

Molecules4

Noninvasive1

Regenerative Medicine1

Quantify5

Migration2

Murine4

Spinal4

Efficacy6

Immune6

Biomarker1

Superparamagnetic1

Approach2

Protein6

Malignancies2

Biology1

Mice10

Apoptosis1

Langerhans2

Mesenchymal1

METHODS2

Biol3

Neuronal1

Therapeutic2

Genes6

Embryonic3

Hematopoietic2

Stem Cell Res1

Subsequently3

Researchers3

Visualize2

Mechanisms3

Cancer cells3

Progeny2

Secrete1

Proliferation1

Populations2

Infection5

Fibroblasts1

Lund University1

Human4

Tumors1