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  • prostatic
  • PSCA is expressed in most prostate cancer specimens, including high-grade prostatic intraepithelial neoplasia, primary androgen-dependent tumors, and hormone-refractory metastases. (aacrjournals.org)
  • TRAMP is a transgenic line of C57BL/6 mice harboring a construct comprised of the minimal -426/+28 rat probasin promoter (426 base pairs of the rat probasin (PB) gene promoter and 28 base pairs of 5'-untranslated region) to target expression of the SV40 large T antigen to prostatic epithelium. (atcc.org)
  • SPAS-1 (stimulator of prostatic adenocarcinoma-specific T cells)/SH3GLB2: A prostate tumor antigen identified by CTLA-4 blockade. (nih.gov)
  • stromal
  • The 150-kDa polypeptide was expressed by LNCaP and PC-3 PCA cells, as well as by normal prostate epithelial cells, but not by prostate stromal cells. (aacrjournals.org)
  • Bone marrow stromal cell antigen 2 (BST-2) is a cellular factor that restricts the egress of viruses such as human immunodeficiency virus (HIV-1) from the surface of infected cells, preventing infection of new cells. (biomedcentral.com)
  • One contributing factor to the failure of systemic therapies may be the abundant tumor stromal content that is the characteristic of PDA. (aacrjournals.org)
  • vaccine
  • A distinctive eosinophilic vasculitis was evident near autologous tumor cells at vaccine sites, and at DTH sites. (aacrjournals.org)
  • HPV16 RNA-LPX vaccine mediates complete regression of aggressively growing HPV-positive mouse tumors and establishes protective T cell memory. (nih.gov)
  • This study describes a novel vaccine approach that facilitates delivery of viral or tumor antigens to dendritic cells in vivo . (asm.org)
  • Moreover, highly efficient antigen delivery to human DCs with recombinant human coronavirus 229E and specific stimulation of human CD8 + T cells revealed that this approach is exceptionally well suited for translation into human vaccine studies. (asm.org)
  • Patients undergo T cell harvest 10 days after an initial priming peptide-pulsed antigen presenting cell (APC) vaccine is performed. (clinicaltrials.gov)
  • The development of sipuleucel-T vaccine was based on the concept of antigen-presenting cells (APCs). (ptcommunity.com)
  • mechanisms
  • Campoli M, Ferrone S. HLA antigen changes in malignant cells: epigenetic mechanisms and biologic significance. (springer.com)
  • During the first three days of infection, different innate immune cells (e.g. monocytes, neutrophils, NK cells, dendritic cells) mediate bactericidal mechanisms that minimize Listeria proliferation. (jove.com)
  • The cell lines can be used in studies to elucidate molecular mechanisms associated with the initiation, progression and metastasis of prostate cancer. (atcc.org)
  • viral
  • We treated MMTV-producing GR cells with IFNα or vehicle and then measured BST-2 mRNA and surface protein levels, as well as extra- and intra- cellular viral particles. (biomedcentral.com)
  • metastasis
  • We have reported previously that anti-PSCA monoclonal antibody (mAb) 1G8 inhibits tumor growth, prevents metastasis, and prolongs the survival of mice inoculated with human prostate cancer cell lines and xenografts. (aacrjournals.org)
  • Recent work has revealed that the PDA stroma supports tumor growth and promotes metastasis and simultaneously serves as a physical barrier to drug delivery. (aacrjournals.org)
  • metastases
  • Comparison of Claudin 18.2 expression in primary tumors and lymph node metastases in Japanese patients with gastric adenocarcinoma. (nih.gov)
  • Using the MMTV/PyVmT transgenic model of metastatic breast cancer, we show that administration of ionizing radiation or doxorubicin caused increased circulating levels of TGF-beta1 as well as increased circulating tumor cells and lung metastases. (nih.gov)
  • Radiation and chemotherapy increase circulating tumor cells and lung metastases. (nih.gov)
  • macrophages
  • Infiltrating leukocyte populations are similar to those found in human breast carcinomas, including the presence of αβ and γδ T cells, macrophages and MDSCs. (jove.com)
  • tissues
  • Effective intranasal immunisation strategies suggest the prospect of protection from inhaled antigens within the respiratory and nasal mucosa and also throughout other mucosal tissues that together comprise the common mucosal immune system 1 , 2 . (wiley.com)
  • The expression of B7-H1 protein is limited to macrophage lineage of cells in normal tissues, although its mRNA transcription is found in a broad range of tissues. (nih.gov)
  • Oncolytic viruses, which are optimized to kill cancer cells without harming the surrounding normal tissues, have gathered increasing interest in recent years. (sciencemag.org)
  • secrete
  • We have previously described a long-lived population of virus-specific antibody-secreting cells (ASC) found within the NALT that actively secrete antibody for the life of the animal after just one infection with influenza virus 4 . (wiley.com)
  • antiviral
  • Our data provide evidence that BST-2 restricts MMTV release from naturally infected cells and that BST-2 is an antiviral factor in vivo . (biomedcentral.com)
  • While the antiviral activity of BST-2 has been demonstrated in tissue culture cells, there has been no evidence that BST-2 exerts antiviral activity in vivo . (biomedcentral.com)
  • cancer
  • In a Phase I human gene therapy trial, eight immunocompetent prostate cancer (PCA) patients were treated with autologous, GM-CSF-secreting, irradiated tumor vaccines prepared from ex vivo retroviral transduction of surgically harvested cells. (aacrjournals.org)
  • nonetheless, infected cells do not produce high virus titer, and time to MMTV-induced cancer is rather long, suggesting that virus replication and spread may be restricted by host factors like BST-2. (biomedcentral.com)
  • We conducted a first-in-man clinical trial of TCR gene-transduced T-cell transfer in patients with recurrent MAGE-A4-expressing esophageal cancer. (aacrjournals.org)
  • This article describes the first TCR gene T-cell therapy for esophageal cancer. (aacrjournals.org)
  • These findings should advance our current knowledge in devising antitumor immunotherapeutic remedies to complement virotherapy-mediated tumor destruction and restrict cancer remission in clinical settings. (aacrjournals.org)
  • ref. 31 ) cell lines were kindly provided by Dr. J. Xiang (Saskatchewan Cancer Agency, Saskatoon, Saskatchewan, Canada) and Dr. E. Podack (University of Miami School of Medicine, Miami, FL), respectively. (aacrjournals.org)
  • CAR T cells are genetically engineered T cells with major histocompatibility complex-independent, tumor-specific, immune-mediated cytolytic actions against cancer cells. (springer.com)
  • In this review article, we highlight the current knowledge regarding CAR T cell therapy in ovarian cancer. (springer.com)
  • We have discussed basic functioning of CAR T cells, their rationale and clinical outcome in ovarian cancer with limitations. (springer.com)
  • June CH. Adoptive T cell therapy for cancer in the clinic. (springer.com)
  • A phase I clinical trial of adoptive T cell therapy using IL-12 secreting MUC-16(ecto) directed chimeric antigen receptors for recurrent ovarian cancer. (springer.com)
  • Cancer Cell. (springer.com)
  • Identification of responder of gastric cancer patients for anti-PD-1 antibody therapy by quantification the capacity of CD28 molecules on the surface of T cells. (nii.ac.jp)
  • Harnessing Tumor Mutations for Truly Individualized Cancer Vaccines. (nih.gov)
  • We hypothesized that both frequency and expression levels of ABC transporters in breast tumors would vary by cancer subtype, and be associated with prognosis. (jove.com)
  • prostate
  • Sera from three of eight vaccinated men contained new antibodies recognizing polypeptides of 26, 31, and 150 kDa in protein extracts from prostate cells. (aacrjournals.org)
  • No antibodies against prostate-specific antigen were detected. (aacrjournals.org)
  • Prostate stem cell antigen (PSCA), a 123-amino acid cell surface glycoprotein, is highly expressed in both local and metastatic prostate cancers as well as in a large proportion of bladder and pancreatic cancers. (aacrjournals.org)
  • These results were confirmed in in vivo models in which both 1G8 and F(ab′) 2 fragments were able to inhibit prostate tumor formation and growth equally. (aacrjournals.org)
  • The advent of prostate-specific antigen (PSA) screening in the 1980s has led to considerable stage migration with increasing numbers of cancers being diagnosed at a potentially curable juncture. (aacrjournals.org)
  • Prostate stem cell antigen (PSCA) is a member of the Thy-1/Ly-6 family of glycosylphosphatidylinositol (GPI)-anchored cell surface antigens ( 1 ). (aacrjournals.org)
  • The TRAMP-C1 (ATCC- CRL-2730), TRAMP- C2 (ATCC-CRL-2731) and TRAMP-C3 ( ATCC CRL-2732 ) cell lines were derived in 1996 from a heterogeneous 32 week primary tumor in the prostate of a PB-Tag C57BL/6 (TRAMP) mouse. (atcc.org)
  • Spas-1 expression is not restricted to the prostate but is increased in older TRAMP tumors. (nih.gov)
  • B ) Mouse Spas-1 expression in normal versus tumor prostate: the level of Spas-1 expression was determined in a panel of normal prostates and primary TRAMP tumors from 21- and 27-week-old mice. (nih.gov)
  • This process activates the APCs, which are now ready to fight the cancerous prostate cells. (ptcommunity.com)
  • significantly inhibited
  • However, conjugates of two derivatives, α 3 ı and N -acetyl-γ 1 ı, in which the rhamnose residue is present but the amino sugar residue of the parent drug is either missing or modified, significantly inhibited tumor growth over a 4-fold dose range and produced long-term tumor-free survivors. (aacrjournals.org)
  • peptide
  • Following chemotherapy, infusion of harvested T cells followed by infusion of peptide-pulsed APC vaccinations occurs every 6 weeks for a total of 3 post-priming vaccinations. (clinicaltrials.gov)
  • Interleukin -2 (IL-2) is administered as a continuous intravenous (IV) infusion for 4 days/week for 3 successive weeks starting on the same day as T cell /peptide-pulsed infusions. (clinicaltrials.gov)
  • We show that the immunodominant SPAS-1 epitope SNC9-H(8) arose from a point mutation in one allele of the gene in TRAMP tumor cells, and that immunization with dendritic cells pulsed with SNC9-H(8) peptide resulted in protection against TRAMP-C2 tumor challenge. (nih.gov)
  • recombinant
  • Furthermore, human DCs transduced with Melan-A-recombinant human coronavirus 229E efficiently activated tumor-specific CD8 + T cells. (asm.org)
  • Sipuleucel-T is composed of recombinant antigen protein, which must be incubated with the patient's isolated APCs ex vivo . (ptcommunity.com)
  • At the facility, the harvested APCs are incubated with recombinant fusion protein antigen, which contains both PAP and GM-CSF. (ptcommunity.com)
  • epithelial cells
  • Five weeks later, lungs from irradiated mice and controls were harvested and lysates (250 μg/ml) added in triplicate wells to mink lung epithelial cells that stably express a plasminogen activator inhibitor-1/luciferase reporter (PAI-1/luciferase reporter). (nih.gov)
  • lymph
  • After intradermal injection, liposomes formed antigen depots which facilitated long-lasting in vivo antigen loading of dendritic cells almost exclusively in the local draining lymph nodes. (uzh.ch)
  • After 7-8 weeks post-adenovirus injection, vasculature is observed connecting the tumor mass to distal lymph nodes, with eventual lymphovascular invasion of YFP+ tumor cells to the distal axillary lymph nodes. (jove.com)