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  • Toxicity
  • OBJECTIVE: The aim of this study was to determine the direct in vitro toxicity of infliximab. (biomedsearch.com)
  • As a proof of principle the in vitro toxicity of thiopurines and methotrexate was also determined. (biomedsearch.com)
  • In vitro toxicity assays have shown the targeting efficiency of these nanoparticles and the synergistic effect of the two drugs are higher than a single drug delivery system. (nsti.org)
  • Purified parasporal inclusions of the three selected strains, designated 84-HS-1-11, 89-T-26-17 and 90-F-45-14, exhibited no haemolytic activity and no insecticidal activity against dipteran and lepidopteran insects, but were highly cytocidal against leukaemia T cells and other human cancer cells, showing different toxicity spectra and varied activity levels. (wiley.com)
  • vivo
  • Although in vivo tests are usually adopted on the study of environmental toxicology, in order to control the influencing factors better and explore further mechanisms, is in vitro cell culture more suitable for the study of environmental-induced oxidative stress? (omicsonline.org)
  • This was true both when the antigen was given in vivo together with the endotoxin as well as when the cells were exposed to antigen in vitro . (rupress.org)
  • However, the effects of crocin on HL-60 cells in vivo have not been evaluated. (hindawi.com)
  • This study aimed to examine the effects of crocin on HL-60 cells in vitro and in vivo and investigate the underlying mechanisms. (hindawi.com)
  • However, the effects of crocin on HL-60 cells in vivo have not been evaluated, and the mechanism responsible for the antileukemia effects of saffron remains elusive. (hindawi.com)
  • In the present study, a series of experiments were performed to examine the effects of crocin on HL-60 cells in vitro and in vivo and investigate the underlying mechanisms. (hindawi.com)
  • Pieces of mouse embryonic pancreatic epithelium cultured in an inductive situation in vitro , or when examined at critical times in vivo , show a gradient of zymogen granule accumulation. (rupress.org)
  • Cells located internally in explants, or in central acini in vivo , show this overt differentiation first. (rupress.org)
  • The appearance of the endothelial cells following EGF stimulation is similar to migrating cells closing a wound in vivo. (arvojournals.org)
  • cytotoxicity
  • METHODS: Cell survival curves and the half maximal inhibitory concentrations (IC(50)) were obtained after 24, 48 and 72 h of incubation in HepG2 cells with the IBD drugs azathioprine, 6-mercaptopurine, 6-thioguanine, methotrexate or infliximab by using the WST-1 cytotoxicity assay. (biomedsearch.com)
  • RESULTS: No in vitro hepatotoxicity in HepG2 cells was seen with infliximab, while concentration-dependent cytotoxicity was observed when HepG2 cells were incubated with increasing concentrations of azathioprine, 6-mercaptopurine and 6-thioguanine. (biomedsearch.com)
  • Of 1684 B. thuringiensis strains having no haemolytic activity, 42 exhibited in vitro cytotoxicity against leukaemia T cells. (wiley.com)
  • assay
  • Changes in density of viable cells after exposure were measured using the MTT assay. (niom.no)
  • HPMA exposure resulted in reduced SDH activity (MTT assay), altered cell growth pattern and increased cell death in a dose dependent manner in all investigated cell lines. (niom.no)
  • epithelial cells
  • Background: Previously we showed that reduced availability of the essential amino acid tryptophan per se attenuates post-transcriptional control of interleukin (IL)-6 and IL-8 leading to hyperresponsive production of these inflammatory mediators by airway epithelial cells. (uva.nl)
  • Epithelial cells were exposed to these polycationic proteins and L-C-14-arginine uptake was assessed as well as IL-6 and IL-8 production. (uva.nl)
  • Conclusion: We conclude that both reduced arginine availability per se and the presence of polycationic proteins may promote airway inflammation by enhanced pro-inflammatory mediator production in airway epithelial cells, but due to distinct mechanisms. (uva.nl)
  • Tumor necrosis factor- α stimulates gingival epithelial cells to release high mobility-group box 1," Journal of Periodontal Research , vol. 43, no. 1, pp. 76-83, 2008. (hindawi.com)
  • Dye coupling of rabbit ciliary epithelial cells in vitro. (arvojournals.org)
  • Microiontophoretic dye injection in a bathing solution with a high Ca2+ (10 mM) or an acidic pH (6.3) completely inhibited cell-cell dye coupling in the rabbit ciliary epithelial cells. (arvojournals.org)
  • viability
  • However, no continuous coral cell lines have been developed to date, and maintenance of primary cell cultures has encountered problems such as short-term viability or contamination by unicellular eukaryotic organisms, which eventually overgrow the original coral cells ( 8 - 13 ). (pnas.org)
  • This study was to investigate the effect of Nidus Vespae decoction (NVD) on tumor cell viability and immunoregulating functions of human peripheral blood immune cells. (hindawi.com)
  • Cell viability was analyzed by the crystal violet method. (hindawi.com)
  • Geopropolis cytotoxic action was exclusively due to its constituents since 70% ethanol (its solvent) had no effect on cell viability. (hindawi.com)
  • physiology
  • In this system, which significantly facilitates studies of coral cell physiology, we demonstrate in vitro excretion of ECM by primary (nondividing) tissue cultures of both soft ( Xenia elongata ) and hard ( Montipora digitata ) corals. (pnas.org)
  • Here we report on the development of a culturing system that significantly facilitates studies of coral cell physiology. (pnas.org)
  • growth
  • In a study of the effect of L-dopa, an intermediate in the biosynthesis of the pigment melanin, on the growth of human and murine melanoma cells a highly selective inhibition of growth was observed for pigmented cell lines (S91A and human melanoma) as compared to the nonpigmented control cells (amelanotic melanoma S91B, mouse fibroblast L929, and Chinese hamster ovary). (sciencemag.org)
  • Using an in vitro culture system, we investigated the effects of five antiglaucoma drugs on growth and morphologic characteristics of bovine trabecular meshwork cells. (nih.gov)
  • Epinephrine hydrochloride (55-550 microM) and pilocarpine hydrochloride (0.8-16 mM), when added to the cultures for 3 days, inhibited trabecular cell growth in a dose-dependent manner. (nih.gov)
  • These results showed that antiglaucoma drugs, depending on their concentrations, may profoundly influence the growth and activity of trabecular meshwork cells. (nih.gov)
  • Xiang G, Yi Y, Weiwei H, Weiming W. TGIF1 promoted the growth and migration of cancer cells in nonsmall cell lung cancer. (springer.com)
  • Data showed that NVD has no significant inhibiting effects on gastric cancer cells growth. (hindawi.com)
  • In the present study, the inhibitory effect of amygdalin on the growth of renal cell carcinoma (RCC) cells was examined. (spandidos-publications.com)
  • Functional blockade by siRNA was used to determine the impact of several proteins on tumor cell growth. (spandidos-publications.com)
  • Furthermore, amygdalin induced a marked decrease in cell cycle activating proteins, in particular cdk1 and cyclin B. Functional blocking of cdk1 and cyclin B resulted in significantly diminished tumor cell growth in all three RCC cell lines. (spandidos-publications.com)
  • Hence, exposing RCC cells to amygdalin inhibited cell cycle progression and tumor cell growth by impairing cdk1 and cyclin B expression. (spandidos-publications.com)
  • The spindle-shaped morphological change did not occur in response to other growth factors, was not related to cell density, and was reversible within 24 hr after removal of EGF from the media or subculture in the absence of EGF. (arvojournals.org)
  • Treatment with EDCL significantly inhibited cell growth in a concentration- and time-dependent manner by inducing apoptosis. (hindawi.com)
  • tissue culture
  • Peritoneal exudate cells produced hemagglutinating antibody to bovine gamma globulin (BGG) in a replicating tissue culture system for approximately 3 weeks when taken from animals given either primary or secondary injections of BGG. (rupress.org)
  • Furthermore
  • Furthermore, the proteins from 84-HS-1-11 and 89-T-26-17 were able to discriminate between leukaemia and normal T cells, specifically killing the former cells. (wiley.com)
  • Furthermore, HL-60 cells were xenografted into nude mice and treated by crocin, the tumor weight and size were calculated, and the expression of Bcl-2 and Bax in xenografts was detected by immunohistochemical staining. (hindawi.com)
  • Furthermore, EDCL did not alter the expression of bax in HepG2 cells but did selectively downregulate the expression of bcl-2 and bcl-xl, resulting in an increase in the ratio of bax:bcl-2 and bax:bcl-xl. (hindawi.com)
  • particles
  • We further demonstrate in vitro production of SOM and extracellular mineralized particles in cell cultures of M. digitata . (pnas.org)
  • Extracellular production of organic matrices and calcium carbonate particles was examined in primary, nondividing cell cultures of the soft coral Xenia elongata and the stony coral Montipora digitata . (pnas.org)
  • Stable TGIF-silenced A549 cells were established by infecting shRNA lentiviral particles. (springer.com)
  • Drugs
  • These drugs caused a reduction in cell density, respectively, at concentrations higher than 103, 460, and 616 microM. (nih.gov)
  • To address this problem, we have explored a C 60 fullerene-based nanosized system as a carrier for anticancer drugs for an optimized drug delivery to leukemic cells. (springer.com)
  • culture
  • This Request for Applications (RFA), Papillomavirus In Vitro Cell Culture Systems, is related to the priority area of sexually transmitted diseases. (nih.gov)
  • ELIGIBILITY REQUIREMENTS Applications may be submitted by domestic and foreign for-profit or nonprofit organizations, public and private, such as universities, medical colleges, hospitals, laboratories, units of State and local governments, and eligible agencies of the Federal government having adequate resources or access to resources to support papillomavirus in vitro cell culture research. (nih.gov)
  • Frank Cerra, professor of surgery at the University of Minnesota, explains that the new technique uses a bioreactor - a system that preserves cells in culture so that they can continue to function. (newscientist.com)
  • In vitro Cell Culture Model for Toxic Inhaled Chemical Testing. (ndriresource.org)
  • Is in vitro Cell Culture Suitable for Study of Environmental-Induced Oxidative Stress? (omicsonline.org)
  • Establishing an in vitro coral cell culture could potentially circumvent these complications and also serve as a model for studying physiological processes at a cellular level. (pnas.org)
  • Cell Culture Characterization. (pnas.org)
  • Methods: IL-6 and IL-8 were determined by ELISA in culture supernatants of NCI-H292 airway epithelial-like cells and normal bronchial epithelial (NHBE) cells that were exposed to TNF-alpha, LPS or no stimulus, in medium with or without arginine. (uva.nl)
  • significantly
  • There are structural differences between the ECM produced by X. elongata cell cultures and that of M. digitata , and ascorbic acid, a critical cofactor for proline hydroxylation, significantly increased the production of collagen in the ECM of the latter species. (pnas.org)
  • concentrations
  • Different cell cultures were exposed to different concentrations of HPMA (0-8 mM) for 24 hours. (niom.no)
  • In leukemic cell lines (CCRF-CEM, Jurkat, THP1 and Molt-16), the nanocomplexes revealed ≤ 3.5 higher cytotoxic potential in comparison with the free drug in a range of nanomolar concentrations. (springer.com)
  • Results: For both NCI-H292 and NHBE cells, low arginine concentrations enhanced basal epithelial IL-6 and IL-8 production and synergized with TNF-alpha-induced IL-6 and IL-8 production. (uva.nl)
  • HepG2
  • Infliximab exerts no direct hepatotoxic effect on HepG2 cells in vitro. (biomedsearch.com)
  • These results support a mechanism whereby EDCL induces apoptosis through the mitochondrial pathway and caspase activation in HepG2 cells. (hindawi.com)
  • Kyoung Jin Nho, Jin Mi Chun, and Ho Kyoung Kim, "Ethanol Extract of Dianthus chinensis L. Induces Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells In Vitro ," Evidence-Based Complementary and Alternative Medicine , vol. 2012, Article ID 573527, 8 pages, 2012. (hindawi.com)
  • fibroblasts
  • The outward transport process for steroids has been found in mouse fibroblasts, mouse lymphoma cells, and functional mouse adrenal gland tumor cells growing in vitro. (rupress.org)
  • The transport process is not present in two varieties of cells cultured from human sources-HeLa or diploid fibroblasts, WI-38. (rupress.org)
  • morphology
  • Cell fixation is an essential approach for preserving cell morphology, allowing the targeting and labelling of biomolecules with fluorescent probes. (archives-ouvertes.fr)
  • One of the key requirements for more efficient fluorescent labelling is the preservation of cell morphology, which usually requires a combination of several fixation techniques. (archives-ouvertes.fr)
  • Geopropolis was efficient against canine OSA cells in a dose- and time-dependent way, leading to a distinct morphology compared to control. (hindawi.com)
  • main
  • The main efforts in cancer research aim at finding more powerful and selective ways for direct elimination of cancer cells. (springer.com)
  • explants
  • Phillips, R. & Torrey, J. G. (1973) DNA synthesis, cell division and cytodifferentiation in cultured pea root cortical explants. (springer.com)
  • In older explants, thymidine incorporation is largely restricted to the periphery of the tissue as zymogen appears in the internal cells. (rupress.org)
  • If explants are treated continuously from 72 to 120 hours, on the other hand, zymogen still forms in some internal cells. (rupress.org)
  • epithelium
  • The presence of a post-DNA-synthetic population is seen in the form of a group of nonlabeling central cells that remains intact in the midst of a labeled epithelium for as long as 48 hours in vitro (from 72 to 120 hours). (rupress.org)
  • PURPOSE: To examine the in vitro properties of gap junctions on the isolated rabbit ciliary epithelium. (arvojournals.org)
  • RESULTS: Dye spread was observed throughout both layers (horizontally and vertically) of the ciliary epithelium adjacent to the injected cell that was more intensely labeled. (arvojournals.org)
  • Extracellular acidosis and high concentration of extracellular Ca2+ cause loss of cell-cell coupling in the rabbit ciliary epithelium. (arvojournals.org)
  • Methods
  • In the second part of this paper experiments are detailed which prove that cells preserved by the methods here recorded function excellently when reintroduced into the body. (rupress.org)
  • Due to demonstrated high accuracy and non-invasiveness, electrochemical detection methods have been used for cell-based investigation. (mdpi.com)
  • METHODS: Intracellular potential was measured and lucifer yellow (5% in 1 M LiCl) was iontophoretically injected into a ciliary epithelial cell. (arvojournals.org)
  • differentiation
  • It is hoped that this paper has summarized the background of xylem cell differentiation studies and has set the scene for the papers to follow in this issue. (springer.com)
  • It is hoped that further advancement of our knowledge in the study of xylem cell development will help understanding in the field of cell differentiation in general. (springer.com)
  • Dodds, J. H. (1979) Is cell cycle activity necessary for xylem cell differentiation? (springer.com)
  • Dodds, J. H. (1981) The role of the cell cycle and cell division in xylem differentiation. (springer.com)
  • Dodds, J. H. (1982) The role of the cell cycle in differentiation, a new model. (springer.com)
  • Fosket, D. E. (1968) Cell division and the differentiation of wound vessel members in cultured stem segments of Coleus . (springer.com)
  • As the epithelia age, the more peripheral cell population proceeds in a similar differentiation. (rupress.org)
  • Some daughter cells apparently return peripherad to divide again, whereas others remain centrally where they undergo differentiation. (rupress.org)
  • proteins
  • Parasporal inclusion proteins from a total of 1744 Bacillus thuringiensis strains, consisting of 1700 Japanese isolates and 44 reference type strains of existing H serovars, were screened for cytocidal activity against human leukaemia T cells and haemolytic activity against sheep erythrocytes. (wiley.com)
  • Expression of the cell cycle activating proteins cdk1, cdk2, cdk4, cyclin A, cyclin B, cyclin D1 and D3 as well as of the cell cycle inhibiting proteins p19 and p27 was examined by western blot analysis. (spandidos-publications.com)
  • effects
  • Effects of antiglaucoma medications on bovine trabecular meshwork cells in vitro. (nih.gov)
  • Conventional anticancer chemotherapy is limited because of severe side effects as well as a quickly evolving multidrug resistance of the tumor cells. (springer.com)
  • These results suggest that NVD appears to have an immunoenhancing effect on immune cells, indicating that Nidus Vespae is worth exploring for immunomodulatory effects in tumor treatment. (hindawi.com)
  • human
  • In citrated blood, as such, human red cells tend to break down rather rapidly, no matter what the proportion of citrate. (rupress.org)
  • But in a mixture of 3 parts of human blood, 2 parts of isotonic citrate solution (3.8 per cent sodium citrate in water), and 5 parts of isotonic dextrose solution (5.4 per cent dextrose in water), the cells remain intact for about 4 weeks. (rupress.org)
  • Genomic structure, alternative splicing and expression of TG-interacting factor, in human myeloid leukemia blasts and cell lines. (springer.com)
  • Dobashi Y. Cell cycle regulation and its aberrations in human lung carcinoma. (springer.com)
  • Human leukemia HL-60 cells were gifted from the Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences, Tianjin. (hindawi.com)
  • The Human Red Cell in Vitro. (annals.org)
  • Functional characterization of the parathyroid hormone 1 receptor in human periodontal ligament cells," Clinical Oral Investigations , vol. 18, no. 2, pp. 461-470, 2014. (hindawi.com)
  • functional
  • Cellular biochemical parameters can be used to reveal the physiological and functional information of various cells. (mdpi.com)
  • Survival
  • The addition of a little gelatin (⅛ per cent) to the wash fluid suffices for this purpose, and by its use the period of survival in salt solutions of washed rabbit, sheep, and dog cells is greatly prolonged. (rupress.org)
  • Carboplatin-induced gene expression changes in vitro are prognostic of survival in epithelial ovarian," BMC Medical Genomics , vol. 1, article 59, 2008. (hindawi.com)
  • Extracellular
  • The evolution of multicellularity in animals required the production of extracellular matrices that serve to spatially organize cells according to function. (pnas.org)
  • species
  • The fragility of the red cells, as indicated by washing or shaking them in salt solution is different, not only for different species, but for different individuals. (rupress.org)
  • Different preservative mixtures are required for the cells of different species. (rupress.org)
  • biological
  • Its biological properties have not been investigated, such as propolis, and herein its cytotoxic action on canine osteosarcoma (OSA) cells was evaluated. (hindawi.com)
  • When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring. (mdpi.com)
  • Rabbit
  • It is greatest in the case of dog corpuscles, and well marked with sheep and rabbit cells. (rupress.org)
  • biology
  • New techniques developed in the area of cell biology should prove useful tools in further study of this most interesting area of cytodifferentiation. (springer.com)
  • protective
  • Though gelatin acts as a protective for red cells it is not preservative of them in the real sense. (rupress.org)
  • Under inflammatory and hyperglycemic conditions, RANKL contributed to the promotion of pro-calcific signalling, whilst TRAIL exhibited a protective influence on vascular cells. (dcu.ie)
  • efficiency
  • The results of this study indicated that C 60 fullerene-based delivery nanocomplexes had a potential value for optimization of doxorubicin efficiency against leukemic cells. (springer.com)
  • bovine
  • HL-60 cells were cultured in RPMI-1640 medium (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS) in a humidified incubator of 5% CO2 at 37°C. Crocin was purchased from Sigma (CAS Number 42553-65-1) and diluted in 10 mmol/L phosphate-buffered saline for the appropriate concentration upon used. (hindawi.com)
  • cultures
  • Cell elongation was seen in cultures treated with epinephrine and dipivefrin, whereas levobunolol and timolol induced the cells to adopt a rounded appearance. (nih.gov)
  • study
  • The objective of the current study was to explore possible cellular responses to the methacrylate monomer 2-hydroxy-propylmethacrylate (HPMA) in vitro . (niom.no)
  • analysis
  • Analysis of the NIR luminescence properties of these MCs was performed under different experimental conditions, including in a suspension of stained cells. (archives-ouvertes.fr)
  • Wang J, Wu C, Hu N, Zhou J, Du L, Wang P. Microfabricated Electrochemical Cell-Based Biosensors for Analysis of Living Cells In Vitro . (mdpi.com)
  • Doxorubicin
  • Using prostate cancer as a model, we report the drug release of pacitaxel and doxorubicin from aptamer-poly (D,L lactic co glycolic acid)-poly(ethylene glycol) (PLGA-PEG) nanoparticles used to target prostate-specific membrane antigen (PSMA), a well characterized antigen that is expressed on the surface of prostate cancer cells. (nsti.org)
  • phagocytosis
  • By scanning electron microscopic techniques, epinephrine, timolol, and levobunolol were found to retard the phagocytosis of latex beads by trabecular meshwork cells. (nih.gov)
  • sheep erythrocytes
  • Antibody produced by the cells was assayed by an hemagglutination technique in which the antigens used were adsorbed to formalinized tanned sheep erythrocytes. (rupress.org)
  • Cancer
  • Cancer is a disease of the cells, which are the body's basic building blocks. (cancervic.org.au)
  • Cancer Cell. (springer.com)
  • These targeted drug delivery nanoparticles could be used as a powerful therapeutic tool, in comparison to the single drug approach, to combat cancer cells displaying drug resistance. (nsti.org)
  • Renal cell carcinoma (RCC) is the most common renal cancer and most aggressive urologic tumor, and its incidence is increasing ( 1 ). (spandidos-publications.com)
  • results
  • Our results demonstrate the ability of isolated, differentiated coral cells to undergo fundamental processes required for multicellular organization. (pnas.org)