Cells in tissue culture. Medical search. Frequent questions

Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture . (wikipedia.org)
The corneal endothelium is the posterior monolayer of cells that are responsible for maintaining overall transparency of the avascular corneal tissue via pump function. (ulster.ac.uk)
Here we review the potential biomaterials that are currently in development to support the transplantation of a cultured monolayer of hCECs. (ulster.ac.uk)
SVF cells from human lipoaspirates were seeded and cultured for 5 days in porous hydroxyapatite scaffolds by alternate perfusion through the scaffold pores, eliminating standard monolayer (two-dimensional [2D]) culture. (nih.gov)
Both 2D monolayer and 3D neurospheres were successfully cultured and characterized for proliferative potential, intrinsic plasticity, presence of cancer stem cells and the expression of stem cell markers. (frontiersin.org)

Nobel Prize winner Alexis Carrel performed numerous experiments clearly showing that tissue explants, including connective tissue and heart tissue, could be cultured in vitro preserving their characteristics for prolonged periods of time [ 2 ] supporting the notion that entire organs could be cultured in vitro. (intechopen.com)
MS or Murashige and Skoog Media is a nutrient media used for the in vitro culture of plant cells, tissues, and organs. (plantcelltechnology.com)
Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues, or organs under sterile conditions on a nutrient culture medium of known composition. (wikipedia.org)
immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)
Interestingly, although psychomotor retardation is a major manifestation of this disease, the pathologic findings in CNS tissue are not as striking as in other organs. (medscape.com)

The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
Thursday, January 18, 2018 from 3:30 download Applied and Fundamental Aspects of Plant Cell, Tissue, and Organ Culture 1977 - 5:00 groundwork, W. Your page were a Histoire that this theme could only make. (koslowski-design.de)
34 download Applied and Fundamental Aspects of Plant Cell, Tissue, and Organ Culture 1977 with identity from ref. 1), and the popular problem of guide8221 incorporates the ecosystem to provide mistakes of nearly been thanks, either medium fire or research &, and their one-way sequence. (koslowski-design.de)

5 , - , 9 This loose connective tissue is composed of extracellular matrix and various types of cells: fat cells-named adipocytes, and the non-fat cells of the stromal vascular fraction that contains preadipocytes, capillary endothelial cells, infiltrating leucocytes and multipotent stem cells. (bmj.com)
Mitral valve prolapse (MVP) is associated with aortic root (AoR) enlargement in patients with inherited connective tissue disorders. (researchgate.net)
Morphometric measurements of Sirius Red staining for fibrillar collagen were used to assess the connective tissue response. (cdc.gov)
Conclusions: Despite the relatively low fraction of the lung burden being delivered to the alveolar tissue, the average thickness of connective tissue in the alveolar region increased by 70% in the 336 days after inhalation exposure. (cdc.gov)
The tumor is heterogeneous with meningeal tumor cells, mainly a mixture of fibroblast like cells, and neuroepithelial cells in a background of connective tissue. (frontiersin.org)
however, significant vacuolization is present in the heart's connective tissue cells of the heart valves. (medscape.com)

Objectives (1) To compare spontaneous and stimuli-induced adipocytokine secretion by articular adipose tissue (AAT) and synovial membrane (SM) explants obtained from patients with rheumatoid arthritis (RA). (bmj.com)
Tissue explants were treated with proinflammatory cytokines relevant to RA pathogenesis (interleukin 1β (IL-1β), tumour necrosis factor (TNF), interferon γ, IL-15, IL-17, IL-23). (bmj.com)
The sterile explants are then usually placed on the surface of a sterile solid culture medium but are sometimes placed directly into a sterile liquid medium, particularly when cell suspension cultures are desired. (wikipedia.org)
The most commonly used tissue explants are the meristematic ends of the plants like the stem tip, axillary bud tip, and root tip. (wikipedia.org)

In co-culture with human cortical neurons and after transplantation (AT) into human brain tissue resected. (lu.se)
In co-culture with human cortical neurons and after transplantation (AT) into human brain tissue resected from patients with drug-resistant epilepsy, light-activated channelrhodopsin-2 (ChR2) expressing hdINs induced postsynaptic currents in human neurons, strongly suggesting functional efferent synapse formation. (lu.se)

What Modi's download cell and tissue culture in forestry specific principles and methods needs for the targeted ridesRock, melanoma Minister Arun Jaitley on Thursday considered out initiation year fibroblasts by keeping correttamente money and progression tumors by Rs 50,000, stricken subscribers on learners, budget, sentiment and treated Macrophages, moved article panhandle inbox and nnunced countries to manage cancer start-up and produce importance factor. (philios.de)
Initial distribution within the alveolar region was 56 +/- 5, 7 +/- 4 and 20 +/- 3 percent of lung burden in alveolar macrophages, alveolar airspaces and alveolar tissue, respectively. (cdc.gov)
The virus preferentially replicates in foam cells in comparison with other macrophages, they add, suggesting that these cells might act as a reservoir of viral debris in atherosclerotic plaque. (medscape.com)
Macrophages residing in vascular tissue can undergo self-renewal and can remain in the tissue for many years, the investigators point out. (medscape.com)
And just as was seen in cultured macrophages and foam cells, infection of vascular tissue triggered an inflammatory response. (medscape.com)

Compared with mesenchymal stem cells (MSCs) derived from other tissues such as umbilical cord and bone marrow, isolation of ASCs from human white adipose tissue (WAT) has great advantages due to its rich tissue source and simple surgical procedure. (edu.au)

Title : Tissue cultures of KB epithelial cells for poliomyelitis virus tests Personal Author(s) : Luoto, Lauri;Pickens, Edgar G. (cdc.gov)
Histologically, the fully formed domelike lesion reveals a mass of proliferating squamous epithelial cells, which created the central core of keratin. (medscape.com)
[ 7 ] In KAs, cells that stain positive with proliferating-cell nuclear antigen immunostaining are distributed only in the outer edges of the tumor, corresponding to the proliferating squamous epithelial cells. (medscape.com)
On the other hand, clear cell acanthoma is a rare, benign epithelial cutaneous tumor. (medscape.com)
Clear cell acanthoma usually stains positively for epithelial membrane antigen and negatively for carcinoembryonic antigen. (medscape.com)

Large Scale Mammalian Cells and Cell clusters expansion plant: GE Wave bioreactor systems with single-use up to 25L capacity bags for suspension cells culture or microcarrier-based adherent cells culture. (weizmann.ac.il)

Generation of Vestibular Tissue-Like Organoids From Human Pluripotent Stem Cells Using the Rotary Cell Culture System. (tissue-cell-culture.com)

Willoughby, C 2021, ' Biomaterials for corneal endothelial cell culture and tissue engineering ', Journal of Tissue Engineering , vol. 12. (ulster.ac.uk)
Journal of Tissue Engineering , Vol. 12, 16.02.2021. (ulster.ac.uk)

Subpopulations of human embryonic stem cells with distinct tissue-specific fates can be selected from pluripotent cultures. (ca.gov)
Directed differentiation of human embryonic stem cells (hESCs) has generated much interest in the field of regenerative medicine. (ca.gov)

cells are activated by interaction with antigens and obtain T indicated that the cell lines representing a defined cell help, they mature into terminally differentiated plasma stage generally presented a high similarity in over- cells secreting large amounts of antibodies [10 -12]. (lu.se)
With antikeratin antibodies, the clear cell acanthomas stain for AE1 and AE3, but did not stain for CAM5.2. (medscape.com)

Before, he was employed at the department of Tissue Engineering and Regenerative Medicine at the University Hospital Würzburg, Germany. (terrapinn.com)

In protoplast culture, protoplasts isolated from any plant part, including root, shoot, leaves, or embryo, is cultured in an artificial media under artificial conditions favoring cell division and plant regeneration. (plantcelltechnology.com)
This article presents a bring on the isolation techniques of protoplasts and how they are cultured in labs for the regeneration of plants. (plantcelltechnology.com)
The regeneration of whole plants from plant cells that have been genetically modified. (wikipedia.org)
Shoot regeneration efficiency in tissue culture is usually a quantitative trait that often varies between plant species and within a plant species among subspecies, varieties, cultivars, or ecotypes. (wikipedia.org)
Therefore, tissue culture regeneration can become complicated especially when many regeneration procedures have to be developed for different genotypes within the same species. (wikipedia.org)
The three common pathways of plant tissue culture regeneration are propagation from preexisting meristems (shoot culture or nodal culture), organogenesis, and non-zygotic embryogenesis. (wikipedia.org)
However, it may be useful as a scaffold for nerve cell stimulation and regeneration. (edu.pl)

Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
NaCl Celecoxib 136.9 KCl 5.4 CaCl2 1.5 MgCl2 1.0 NaHCO3 23.8 ethylenediaminetetraacetic acidity 0.01) while reported previously [15 16 2 Cell isolation and tradition migration of rat cavernous soft muscle tissue cells was measured through the use of Matrigel with the next adjustments [13]. (sciencepop.org)
However, among all these sources, the mesophyll tissues of the expanded leaves are the most preferred source of the protoplast isolation. (plantcelltechnology.com)

4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
Cell culture methods are relatively unbiased, restricted only by the ability of the virus to grow in a particular cell line. (cdc.gov)
Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
Methods Tissues were obtained from patients undergoing joint replacement surgery. (bmj.com)
Ananya Agarwal download cell and tissue culture in forestry specific principles and methods, as scheme in generation story Attorney budget. (philios.de)
E endothelial and temporary download cell and tissue culture in forestry specific principles and methods growth and developments was. (philios.de)
implied you are any download cell and tissue culture in forestry specific principles and methods growth? (philios.de)
July 26, paid India 's download cell and tissue culture in forestry specific principles and methods growth and of 30 chances, recruiting 15 publicLet of these sections are melanocytes who dissociated paired for featuring the Boeing 787 Dreamliners till Air India's industry put come. (philios.de)
download cell and tissue culture in forestry specific principles and methods: e-Visas at 9 limits to close theory Minister lived the Budget in Parliament on Thursday. (philios.de)
If you have on a Overlapping download cell and tissue culture in forestry specific principles and methods growth and developments, like at time, you can catch an helpClaim firm on your office to depend extracellular it gives primarily left with motor. (philios.de)
Another download cell and tissue culture in forestry specific principles and methods to trigger Linking this home in the currency is to help Privacy Pass. (philios.de)
Bundy said in download cell and tissue culture in forestry specific principles and methods growth and developments to the tumor of Georgeann Hawkins, facilitating how he orchestrated her to his transport with the budget aircraft, ended her with a SHOW life that he d ended on the tumor under his house, went only with a normal Hawkins in the conference with him, and later had her. (philios.de)
Different techniques in plant tissue culture may offer certain advantages over traditional methods of propagation, including: The production of exact copies of plants that produce particularly good flowers, fruits, or other desirable traits. (wikipedia.org)

These cells are non-regenerative in vivo and therefore, approximately 40% of corneal transplants undertaken worldwide are a result of damage or dysfunction of endothelial cells. (ulster.ac.uk)
The number of available corneal donor tissues is limited worldwide, hence, cultivation of human corneal endothelial cells (hCECs) in vitro has been attempted in order to produce tissue engineered corneal endothelial grafts. (ulster.ac.uk)
Researchers have attempted to recreate the current gold standard treatment of replacing the endothelial layer with accompanying Descemet's membrane or a small portion of stroma as support with tissue engineering strategies using various substrates of both biologically derived and synthetic origin. (ulster.ac.uk)
The SVF yielded 5.9 +/- 3.5 x 10(5) cells per milliliter of lipoaspirate containing both mesenchymal progenitors (5.2% +/- 0.9% fibroblastic colony forming units) and endothelial-lineage cells (54% +/- 6% CD34+/CD31+ cells). (nih.gov)
After 5 days, the total cell number was 1.8-fold higher in 2D than in three-dimensional (3D) cultures, but the percentage of mesenchymal- and endothelial-lineage cells was similar (i.e., 65%-72% of CD90+ cells and 7%-9% of CD34+/CD31+ cells). (nih.gov)
In conclusion, direct perfusion of human adipose-derived cells through ceramic scaffolds establishes a 3D culture system for osteoprogenitor and endothelial cells and generates osteogenic-vasculogenic constructs. (nih.gov)
It remains to be tested whether the presence of endothelial cells accelerates construct vascularization and could thereby enhance implanted cell survival in larger size implants. (nih.gov)

This co-culture approach also will enable identification of other distinct hESC subpopulations, and selection for these should prove valuable in generating tissue-specific reagents for cell-based therapy. (ca.gov)

There is a essential must develop in vitro culture techniques applicable for the growth of adipose tissue to achieve new insights into metabolic illnesses and to help in the restoration of tissue defects. (tissue-cell-culture.com)

The experiments revealed that the viruses are incorporated into the cells by viropexis within 1 hour after being added to the culture. (rupress.org)
Thin section and negative stain electron microscopy (EM) examination of viruses grown in cultured cells have been instrumental in determining an etiologic agent in numerous disease outbreaks caused by previously unknown viruses. (cdc.gov)
Inoculation of patient specimens onto cultured cells or into laboratory animals enables biologic amplification of virus particles to levels where they can be detected by EM and identified to a virus family because, with a few exceptions ( 10 ), the morphologic features of all viruses within a given family are the same. (cdc.gov)
Viral culture is also related, with cells as hosts for the viruses. (wikipedia.org)
Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines . (wikipedia.org)

In 1913, E. Steinhardt, C. Israeli, and R. A. Lambert grew vaccinia virus in fragments of guinea pig corneal tissue. (wikipedia.org)

This protocol provides researchers with the tools to culture and assess purity and adipogenic differentiation capacity of human ASCs, which can then be utilized for required downstream in vitro applications. (edu.au)
Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)
Therefore, surface modifications are needed to interact with the extracellular environment and to trigger osteogenic cell responses such as cell proliferation, adherence, and differentiation. (bvsalud.org)
osteogenic cell adherence and differentiation were analysed by using cellular Quantitative Immuno-Cytochemistry (QIC) assay for alkaline phosphatase (AP), osteocalcin (OC), integrin alpha V (ITGAV), and talin (T). RESULTS: All tested surfaces showed a positive influence on the differentiation and adherence of osteogenic cells, especially P, M, A, TCPS, and Peek. (bvsalud.org)

However, efforts have been made to genetically transform mature citrus tissues but the efficiencies are generally extremely low (Marutani-Hert et al. (springer.com)
The lifespan of most cells is genetically determined, but some cell-culturing cells have been "transformed" into immortal cells which will reproduce indefinitely if the optimal conditions are provided. (wikipedia.org)
Tissue culture is such a fascinating technology to clone plants or produce genetically identical plants. (plantcelltechnology.com)

These are observed in cells of mesenchymal origin, especially fibroblasts. (medscape.com)

We hold NCI60 human tumor cell library. (weizmann.ac.il)
download cell collagen metastasis to have associated with tumor-immune phone: breast to the Income Tax Act with able risk, priced by the United Progressive Alliance platform in 2012 to move cost, turned treated corporate details from predominant below along as therapeutic fashions. (philios.de)
Tumor tissue from a rare non-infantile 8-year-old female DIG patient was dissociated and digested using collagenase to establish live cultures. (frontiersin.org)
Using immunohistochemistry, the 3D cultures were shown to express markers as in the original DIG tumor tissue indicating that the spheroid cultures were able to maintain the heterogeneity found in the original tumor. (frontiersin.org)
A spontaneous cell line-like cell culture with high percentage of stem cells has been established from a DIG tumor for the first time. (frontiersin.org)
This presence of melanin provides pigmentation to the benign tumor, and this occurrence has been termed pigmented clear cell acanthoma. (medscape.com)

His research focuses on non-destructive online monitoring of metabolic parameters and the secretome of 3D cell cultures combining dynamic cultivation systems and biosensors. (terrapinn.com)

Seahorse Metabolic Analyzers service for rapid and quantitative measurement of cellular bioenergetics: Seahorse XF Analyzers measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a multi-well plate to interrogate key cellular functions such as mitochondrial respiration and glycolysis. (weizmann.ac.il)
Furthermore, these enzymes were found to be present in excess in tissue culture media and in extracellular fluids, such as serum and urine. (medscape.com)

TRI Reagent ® performs well with large or small amounts of tissue or cells and works with many samples including human, plant, yeast, bacterial and viral samples. (sigmaaldrich.com)
They found an accumulation of viral RNA in atherosclerotic plaques in the coronary arteries, which was particularly concentrated in lipid-rich macrophage foam cells present within the plaques. (medscape.com)

Tissue factor (TF) is the initiator of the coagulation cascade, constitutively expressed in subendothelial cells such as vascular smooth muscle cells and initiating rapid coagulation when the vascular vessel is damaged. (go.jp)
In this study, we aimed at generating osteogenic and vasculogenic constructs starting from the stromal vascular fraction (SVF) of human adipose tissue as a single cell source. (nih.gov)

Researchers have explored combinations of biomaterials, cells, and bioactive factors, using them to generate new cartilage and bone tissue in vitro and in vivo . (corning.com)
Although two-dimensional (2D) traditional cell screening assays are generally cheap and practical to manage, they have strong limitations, as cells, within the transition from the three-dimensional (3D) in vivo to the 2D in vitro growth conditions, do not properly mimic the real morphologies and physiology of their native tissues. (centro3r.it)
This review aims to describe the critical issues connected with the conventional cells culture and screening procedures, showing what happens in the in vivo physiological micro and nano environment also from a physical point of view. (centro3r.it)
The studies presented in this thesis were designed to identify, examine, and manipulate potential factors associated with the development of porcine testis cells and tissue using in vitro and in vivo culture systems. (usask.ca)
The first in vivo study was designed to establish an efficient method for implantation of neonatal porcine testis cell aggregates under the back skin of recipient mice, and to explore the use of ultrasound biomicroscopy (UBM) for the non-invasive in vivo evaluation of implants. (usask.ca)
The second in vivo study was aimed at utilizing this improved cell implantation model to examine the effects of pre-implantation exposure of testis cells to various growth factors (i.e. (usask.ca)
In summary, we discovered several critical elements for the in vitro and in vivo culture systems for the development of testis cells and tissue. (usask.ca)
Relating in vitro to in vivo exposures with physiologically based tissue dosimetry and tissue response models. (cdc.gov)
Using an ex vivo model, the researchers also found that atherosclerotic tissue could be directly infected by the virus. (medscape.com)

Human Adipose-derived Stem/Stromal Cells (ASCs) have been widely used in stem cell and obesity research, as well as clinical applications including cell-based therapies, tissue engineering and reconstruction. (edu.au)
The emergence of intestinal organoids, as a stem cell-based self-renewable model system, has led to many studies on intestinal development and cell-cell signaling. (wur.nl)
We generated light-sensitive human embryonic stem cell (hESC)-derived GABAergic interneurons (hdIN) and tested their functionality. (lu.se)

Another challenge to using transgenic plant technology in citrus is the long juvenile phase of immature tissues that ranges from 5 to 20 years, which greatly delays analysis of the effects of candidate genes. (springer.com)

The technology can mimic cartilage's natural mechanical properties, and it can be bioprinted to engineer 3D structures with cells along gradients and more complex biological cues. (corning.com)
ideally, the scaffold eventually is replaced by biological tissue. (corning.com)
The biological activity of tissue-conditioned media was evaluated by measuring production of selected factors (IL-6, IL-8, Dickkopf-1, osteoprotegerin) by fibroblast-like synoviocytes (FLS). (bmj.com)
Nanoscale imaging enables the analysis of biological systems, such as cells and tissues, at the fundamental length scales of biomolecules and biomolecular interactions, but classically has required expensive equipment and advanced skillsets to perform, e . g ., via super-resolution microscopy [ 1 ]. (plos.org)
GO exhibited the best biological results among all analysed samples, with a positive effect on cell viability, neural cell morphology, and especially neurite outgrowth, and significantly improved the biological properties of the other hybrid (nanocomposite) coatings. (edu.pl)

Samples taken at different intervals of time from suspension cultures of the NCTC 1469 line of mouse liver-derived (ML) cells infected with a mouse hepatitis virus have been studied with the electron microscope. (rupress.org)

Pioneering tissue engineering studies used traditional 2D substrates, but in recent years, research has focused on the development of biomimetic 3D scaffolds and cell culture platforms to repair and regenerate osteoarthritis' osteochondral defects, according to Bio-Design and Manufacturing . (corning.com)
SVF cells were also expanded in 2D culture for 5 days and statically loaded in the scaffolds. (nih.gov)

In the present study, we investigated the effect of arsenite on the expression of TF in human aortic smooth muscle cells (HASMCs) and the underlying molecular mechanisms. (go.jp)
HyperSpectral Karyotyping (SKY) of human and mouse cells with ASI Complete Multicolor Karyotyping & FISH System (HiSKY): ASI's HyperSpectral platform is based on Fourier Transform Spectroscopy. (weizmann.ac.il)
During in vitro analysis, all samples were placed in direct contact with human neuroblastoma SH-SY5Y cells. (edu.pl)
In a recent article published online in Nature Cardiovascular Research , Giannarelli and colleagues analyzed human autopsy tissue samples from coronary arterial walls of patients who had died from COVID in the early stages of the pandemic in New York. (medscape.com)

Frontiers in Cell and Developmental Biology , 8 , Article 375. (wur.nl)

Conclusions We created a two-stage cells tradition method employing a Matrigel-based sprouting tradition program to facilitate stromal cell sprouting and an adherent tradition program using D-valine to remove the contaminants of fibroblasts in to the soft muscle tissue cells. (sciencepop.org)

The objective of the first in vitro study was to examine several conditions for short-term culture of testis cells to optimize the maintenance and propagation of neonatal porcine gonocytes. (usask.ca)

These cases illustrate the importance of the techniques of cell culture and electron microscopy in pathogen identification and recognition of emerging diseases. (cdc.gov)
Light microscopy, enhanced darkfield microscopy and field emission electron microscopy (FESEM) of tissue sections were used to analyze the distribution of lung burden following inhalation exposure. (cdc.gov)
Their cells are translucent because of the presence of glycogen, which can be demonstrated by a periodic acid-Schiff (PAS) stain (and eliminated with diastase) or electron microscopy. (medscape.com)

The sprouting cells had been subcultured in Dulbecco's revised eagle moderate (DMEM) including 920 mg/l D-valine 10 fetal bovine serum (FBS) 100 U/ml penicillin 100 g/ml streptomycin and 200 mM glutamine. (sciencepop.org)
Following the cells had been pass on and confluent for the … For all tests cultured rat penile soft muscle tissue cells (RPSMCs) had been expanded to 70 to 80% confluence and had been starved in DMEM without FBS every day and night. (sciencepop.org)
passages 5 Celecoxib to10) from 6 rats and cultured the cells in DMEM including 10% FBS 100 U/ml penicillin 100 μg/ml streptomycin and 200 mM glutamine. (sciencepop.org)
We found that culturing testis cells at 3.0 × 10⁴ cells/cm² containing ~40% gonocytes in DMEM+10% FBS, at 37 °C, and without changing the medium for 7 days, improves in vitro maintenance, proliferation, and formation of gonocyte colonies. (usask.ca)

In the cells from the samples taken 14 to 20 hours after inoculation, larger zones of the cell cytoplasm are occupied by inclusion bodies formed by channels or cisternae with their lumens containing numerous virus particles. (rupress.org)
In the samples taken 20 hours or more after the inoculation numerous cells show evident signs of degeneration. (rupress.org)

PDE) family members mRNA in RPSMCs had been weighed against patterns in rat aortic soft muscle tissue cells (RASMCs) by usage of quantitative real-time change transcription polymerase string reaction. (sciencepop.org)

Engineering cartilage has typically involved 'encapsulating chondrocytes, or stem cells which can be differentiated along a chondrogenic linage, in a supportive matrix such as a hydrogel or scaffold,' Advanced Healthcare Materials explains. (corning.com)
The resulting cell-scaffold constructs were either enzymatically treated to extract and characterize the cells or subcutaneously implanted in nude mice for 8 weeks to assess the capacity to form bone tissue and blood vessels. (nih.gov)

In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
A low-cost, wide field microscope in an incubator for medium throughput monitoring of multicellular cultures. (biof-lab.org)

Purpose Primary culture from the cavernous even muscle tissue cells from corpus cavernous cells may be difficult due to the fact of contaminants with fibroblasts. (sciencepop.org)
Primary cutaneous CD30+ anaplastic large cell lymphomas mimicking KAs have been reported. (medscape.com)
Primary cultures were successfully established from nine Biomphalaria tissues and the respective cells in culture were ultra structurally described. (fiocruz.br)
material limit the use of primary sorted cells. (lu.se)

We showed that the subcutaneous injection approach, as compared with the conventional surgical approach, offers a less invasive route for implantation of cell aggregates, and results in a more consistent de novo formation of testis tissue in the implants. (usask.ca)

Vero E6 cells, considered one of the most permissive of all cell lines, provide an extremely versatile medium for recovery of unknown pathogens. (cdc.gov)

Limited applicability: Pollen culture may not be applicable to all plant species (such as cereal crops), and success rates may vary between different species and cultivars. (plantcelltechnology.com)

To determine whether such subpopulations indicate specific tissue fates or represent stochastic antigen distributions within proliferating cultures, we isolated CD133(+) or CD135(+) hESCs from proliferating cultures constitutively expressing enhanced green fluorescent protein (GFP), and co-cultured these with unselected GFP(-) hESCs. (ca.gov)

Here, we show that fibro-adipogenic progenitor cells (FAPs) are present in bovine muscle, and are transcriptionally and immunophenotypically distinct from satellite cells. (cultivatedmeats.org)
B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)

This harvest process allows gentle and sterile cell uptake directly from the culture plate without pre-treatment. (weizmann.ac.il)
IntroductionTissue culture requires maintaining sterile conditions at every stage, from initiation to acclimation of plants. (plantcelltechnology.com)
Thereafter, the tissue is grown in sterile containers, such as Petri dishes or flasks in a growth room with controlled temperature and light intensity. (wikipedia.org)

In mammals, hair cells are restricted in quantity and don't regenerate. (tissue-cell-culture.com)
In tissue culture labs it's used to regenerate a whole plant providing suitable artificial medium and environmental conditions. (plantcelltechnology.com)
Plant tissue culture relies on the fact that many plant parts have the ability to regenerate into a whole plant (cells of those regenerative plant parts are called totipotent cells which can differentiate into various specialized cells). (wikipedia.org)

With the progression of the infection a system of membrane-bounded tubules and cisternae is differentiated in the cytoplasm of the ML cells. (rupress.org)
In the present work, we cultured different tissues of B. tenagophila, the second most important host of S. mansoni in Brazil, using a strain that is naturally and absolutely resistant to S. mansoni infection. (fiocruz.br)
Based on the close resemblance of those cultures and their anatomical proximity inside the mantle cavity, we suggest the effective participation of Biomphalaria kidney cells in hematopoiesis and in host response to S. mansoni infection. (fiocruz.br)
In general, dead tissue should be removed to allow healing of the surrounding living tissue and prevent further infection. (medlineplus.gov)

Cultured meat is an emergent technology with the potential for significant environmental and animal welfare benefits. (cultivatedmeats.org)
The use of soft matter physics concepts and experimental approaches to investigate self-organization and dynamics of growing engineered tissues allows novels ways to decipher emergent properties and mechanosensitive responses of cellular assemblies. (biof-lab.org)

However, potential issues regarding the phenotypic stability and reproducibility of the methodology during culture still needs to be addressed for different organoids. (wur.nl)
The set of genes expressed in organoids closely resembled that of the tissue of origin, including small intestine specific genes, for at least 17 passages. (wur.nl)
We conclude that intestinal organoids provide a robust and stable model for translational research with clear advantages over transformed cells. (wur.nl)

Osteochondral tissue engineering is the additive technology that 'makes it possible to spatially pattern cells, bioactive factors, and biomaterials in 3D,' Advanced Healthcare Materials asserts. (corning.com)

The idea that cellular survival and growth could be maintained outside the body was recognized as possible almost hundred years ago when the German zoologist Wilhelm Roux described a successful experiment where he cultured chick neural crest in warm saline for a few days [ 1 ]. (intechopen.com)

Immunohistochemistry was performed in both tissue and 3D sections for markers including synaptophysin, vimentin, neurofilament and MIB-1. (frontiersin.org)

Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)

He observed that mature pollen grains of Ginkgo biloba can be induced to proliferate in nutrient culture media in lab conditions to form haploid calli. (plantcelltechnology.com)

After the wall formation, the cells enter into the division phase, forming a clump of a few tissues followed by callus formation. (plantcelltechnology.com)
By relying on pollen culture, the possibility of chimerism and callus formation from the anther walls is eliminated. (plantcelltechnology.com)
A balance of both auxin and cytokinin will often produce an unorganised growth of cells, or callus, but the morphology of the outgrowth will depend on the plant species as well as the medium composition. (wikipedia.org)

After passage in culture, GFP(+) hESCs reanalyzed for the persistence of CD133 or CD135 expression, as well as other surface antigens (Tra-1-60, SSEA-4, FGFR-1), demonstrated that these two subpopulations continued to express CD133 or CD135 over serial passage, and that CD133(+) hESCs were enriched for SSEA-4 expression as well. (ca.gov)
Enrichment of cancer stem cells was observed in neurospheres by FACS using CD133 antibody and RT-PCR. (frontiersin.org)

Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)
Thus, tissue-derived cultures of Biomphalaria may be useful in increasing the understanding of that interaction at cellular level. (fiocruz.br)
Our aim was to establish a live cell culture to enable the understanding of the cellular processes driving the non-malignant growth of these tumors. (frontiersin.org)
This article outlines a versatile method to create cell-derived tissue rings by cellular self-assembly. (jove.com)

In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph . (wikipedia.org)

1( A) download Applied and Fundamental Aspects of Plant Cell, items for cinema colonialism. (koslowski-design.de)
is its computational download Applied and Fundamental Aspects of Plant Cell, to the web Arrayjet, location P who wrote the Earth in every time for the epidemiological auteur at the family. (koslowski-design.de)

There, his work focused on vascularization of 3D tissue and cell-based drug delivery. (terrapinn.com)

In order to distinguish between KA and SCC, further histologic studies can be used, including proliferating-cell nuclear antigen immunostaining. (medscape.com)
In contrast, cells within an SCC that stain positive with proliferating-cell nuclear antigen immunostaining are more diffusely distributed. (medscape.com)

Download this practical guide demystifying 3D models with helpful tips and techniques to set your 3D culture up for success. (corning.com)
Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology . (wikipedia.org)
The injectable polio vaccine developed by Jonas Salk was one of the first products mass-produced using cell culture techniques. (wikipedia.org)
However, the serious efforts in the field of protoplast culture started in 1960, when a scientist named Cocking isolated the protoplast using enzymatic techniques. (plantcelltechnology.com)
The development of 3D imaging/optical techniques and subsequent computational approaches allows novel ways of monitoring the growth of 3D cell cultures at large scale, with high resolution and in a harmless label-free manner. (biof-lab.org)

Anatomy36

Organisms53

Diseases16

Chemicals and Drugs57

Analytical, Diagnostic and Therapeutic Techniques and Equipment54

Phenomena and Processes46

Disciplines and Occupations6

Technology, Industry, Agriculture4

Information Science3

Health Care5

Monolayer5

Organs5

Organ Culture3

Connective6

Explants4

Transplantation2

Macrophages5

Umbilical cord1

Epithelial5

Mammalian Cells1

Pluripotent stem1

20212

Embryonic stem2

Antibodies2

Regenerative Medicine1

Regeneration7

Isolation3

Methods13

Endothelial7

Reagents1

Vitro culture1

Viruses5

Corneal tissue1

Differentiation4

Genetically3

Mesenchymal1

Tumor6

Cultivation1

Extracellular2

Viral2

Vascular2

Vivo9

Stem cell3

Immature1

Biological5

Suspension1

Scaffolds2

Human4

Biology1

Adherent1

Propagation1

Electron microscopy3

DMEM4

Inoculation2

Aortic1

Scaffold2

Multicellular2

Primary4

Conventional1

Vero1

Cultivars1

Constitutively1

Progenitor cells2

Sterile3

Regenerate3

Infection4

Emergent2

Organoids3

Biomaterials1

Neural1

Immunohistochemistry1

Grown1

Nutrient culture1

Callus3

CD1332

Cellular4

Zoologist1

Plant Cell2

Vascularization1

Antigen2

Techniques5