Cells in peripheral blood structural clone cells. Medical search. Frequent questions

We report on the cloning and expression of a recombinant F. gigantica 14-3-3 epsilon protein (rFg14-3-3e), and testing its effects on specific functions of goat peripheral blood mononuclear cells (PBMCs). (biomedcentral.com)
The FPV can remain latent in peripheral blood mononuclear cells of healthy cats with high virus-neutralizing titers (Ikeda, Miyazawa et al. (bioguardlabs.com)

The importance of cytotoxic T lymphocytes (CTLs) in the immunosurveillance of Epstein-Barr virus (EBV)-infected B cells is firmly established, and the viral antigens of CTL recognition in latent infection are well defined. (silverchair.com)
By stimulating peripheral blood lymphocytes with HLA-B8+ EBV-transformed B lymphoblastoid cells, the primary virus-specific CTL response was shown to include specificities for two HLA-B8-restricted antigenic determinants, FLRGRAYGL and QAKWRLQTL, which are encoded within the latent EBV nuclear antigen EBNA-3. (silverchair.com)
The original monoclonal antibody was generated by sequencing peripheral blood lymphocytes of a patient exposed to the SARS-CoV. (abcam.com)
The T cell antigen receptor complex expressed on normal peripheral blood CD4-, CD8-T lymphocytes. (bdbiosciences.com)
It is indicated for use as an adjunct to standard cytogenetic analysis for identifying and enumerating chromosome 12 via fluorescence in situ hybridization (FISH) in interphase nuclei of cells obtained from peripheral blood lymphocytes in patients with B-cell chronic lymphocytic leukemia (CLL). (molecularcatalog.abbott)
It is not intended for use with test matrices other than peripheral blood lymphocytes from subjects with CLL, to screen for chromosome 12 aneuploidy, eg, in asymptomatic individuals, or to monitor patients for residual disease. (molecularcatalog.abbott)
The CEP 12 assay has been validated only for use with peripheral blood lymphocytes obtained from patients with B-cell CLL leukemia. (molecularcatalog.abbott)
Natural killer (NK) cells are innate lymphocytes that play a pivotal role in the immune surveillance and elimination of transformed or virally infected cells. (frontiersin.org)
Natural killer cells are cytolytic lymphocytes belonging to the innate immune system and are involved in anti-viral and anti-tumor responses ( 1 ) and are recognized as major players in immune-mediated anti-tumor therapies ( 2 ). (frontiersin.org)
Human peripheral blood lymphocytes were stained with CD19 PE and CD1c (clone L161) APC/Fire™ 750 (left) or Mouse IgG1, κ APC/Fire™ 750 isotype control (right). (biolegend.com)
Recent studies indicate that memory T lymphocytes contain distinct populations of central memory (T CM cells) and effector memory (T EM ) cells characterized by distinct homing capacity and effector function. (scielo.br)
In a very interesting review, P. MARRACK & J. KAPPLER focus on naïve, activated, and memory T cells survival separately, and discuss experiments done in vitro and in mice lacking or containing normal populations of lymphocytes. (scielo.br)
Furthermore, MP increased WBC and HGB counts and reduced the ratio of G0/G1 phase obviously, increased the proportion of S phase and G2/M phase in peripheral blood lymphocytes, increased the counts of CD4+ T cells and the ratio of CD4+/CD8+, and reduced the inhibition rate of splenic lymphocytes. (hindawi.com)

Using a chemo-genetic approach, we identify BET bromodomain containing proteins BRD2 and BRD4 as central regulators of NK cell functions, including direct cytokine secretion, NK cell contact-dependent inflammatory cytokine secretion from monocytes as well as NK cell cytolytic functions. (frontiersin.org)
CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. (thermofisher.com)
Used cell isolation techniques to purify PBMCs from blood (Ficoll), select monocytes and CD4 T cells, differentiate and harvest dendritic cells and macrophages. (livecareer.com)
Activation of endothelial cells via antibody-enhanced dengue virus infection of peripheral blood monocytes. (microbiologyresearch.org)
Mice inoculated with myeloid-restricted ZIKV exhibited similar trends as PBS-sham inoculated mice, including a lack of inflammatory cytokine production and a decrease in Ly6C(hi) ZIKV RNA positive monocytes.ImportanceMyeloid cells, including monocytes, play a crucial role in immune responses to pathogens. (cdc.gov)
Monocytes have also been implicated as 'Trojan horses' during viral infections, carrying infectious virus particles to immune privileged sites and/or to sites protected by physical blood-tissue barriers, such as the blood-testis barrier and the blood-brain barrier. (cdc.gov)
Lastly, we identified Ly6C(mid/hi) murine monocytes as the major myeloid cell population that disseminates ZIKV. (cdc.gov)
for example, a particular drug might inhibit kinase activity in T cells but not in monocytes. (genengnews.com)

In this scholarly study, we present proof that MAGI proteins, structural the different parts of AJC whose function continued to be unclear, regulate apical constriction of epithelial cells through the Par polarity proteins. (aboutsciencenow.info)
Therefore, we exposed the MAGIs are crucial regulators of Par polarity proteins that are central towards the rules of pressure distribution in epithelial cells homeostasis. (aboutsciencenow.info)
Additionally, we found ZIKV RNA in seminal leukocytes and epithelial cells. (cdc.gov)
These changes are thought to lead to tumor-microenvironment interactions via factors secreted by stromal or epithelial cells in response to lymphoma cell-produced cytokines ( 7 ). (spandidos-publications.com)
Although these diseases arise from the transformation of different cell types (namely melanocytes, CD4+ T cell and epithelial cells), they share common features such as a partially elucidated pathophysiology, a lack of treatment at advanced stages, a need to understand the mechanisms leading to relapse or resistance upon currently available treatments and consequently a need for alternative therapies. (hipi-lab-saint-louis.fr)

Strategies Chimeric viruses had been produced using V1-V3 envelope fragments from a subtype-A/C dually-infected girl with preferential genital replication of subtype-C. Viral version, spread and cellular fusion ability had been examined using peripheral bloodstream mononuclear cellular material (PBMC) and HeLa-CD4-CCR5 cellular lines, sequencing and cloning. (acp2018.org)
Whenever a V1-5 V3-A316T or deletion was included into subtype-A, each increased viral fusion and spread in cell-lines and PBMC with low CCR5-appearance many fold. (acp2018.org)
Viral RNA was purified utilizing the QIAamp Viral RNA Mini Package(Qiagen), reverse-transcribed and amplified utilizing the SuperScript One-Step RT-PCR Package for Long Web templates(Invitrogen), cloned and sequenced. (acp2018.org)
These immune assays will be advanced by the development of standardized viral stocks, such as HIV-1 infectious molecular clones (IMC), that i) express a reporter gene, ii) are representative of globally diverse subtypes and iii) are engineered to easily exchange envelope (env) genes for expression of sequences of interest. (plos.org)
Decreased neutrophil numbers in the spleen were observed during acute infection with myeloid-restricted ZIKV that precluded the generation of viremia and viral dissemination to peripheral organs. (cdc.gov)
By engineering ZIKV clones to encode myeloid-specific microRNA target sequences, viral replication was inhibited in myeloid cells by harnessing the RNAi pathway. (cdc.gov)
Furthermore, viral dissemination to peripheral organs was not observed in these mice. (cdc.gov)
1995). The pathway of viral entry into cells is not fully characterized, however through the feline transferrin receptor (TfR), FPV binds and uses the receptor to infect feline cells (Parker, Murphy et al. (bioguardlabs.com)
2004). Viral DNA is released from the capsid and replicates through double-stranded RNA intermediates in the nucleus of the cell using the host's DNA polymerase (Barrs 2019). (bioguardlabs.com)
Although NK cells play important roles in host defense against various infectious agents, they are critical in host defense against viral infections. (scielo.br)
provides an overview of the dynamic in vivo life of NK cells from their development in the bone marrow to their mature cellular responses in the periphery and their ultimate demise, with emphasis on mouse NK cells and viral infections. (scielo.br)
Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)
genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)

Using TCR alpha and beta repertoire sequencing for T-cell subsets, as well as single-cell RNAseq and TCRseq, we track the concentrations and phenotypes of individual T-cell clones in response to primary and secondary yellow fever immunization - the model for acute infection in humans - showing their large diversity. (elifesciences.org)
Following inoculation of a highly sensitive immunodeficient mouse model, crucial immune parameters, such as quantification of leukocyte cell subsets, cytokine and chemokine secretion, and viremia were assessed. (cdc.gov)
Dr. Kritzik and colleagues are using phosphor-flow cytometry to study three phosphoproteins-all linked to disease and all potential drug targets-in subsets of peripheral blood cells. (genengnews.com)
discuss the role of integrins in T cell- mediated immunity, with a focus on how these receptors participate in lymphocyte recirculation and T cell activation, how antigen stimulation regulates integrin activity, and how integrins define functionally unique subsets of T cells and APCs. (scielo.br)
The mechanism was perhaps related to inhibiting lymphocyte apoptosis and promoting the balance of Th1/Th2 cell subsets. (hindawi.com)

Because substantial interclonal variance exists in relative virulence, molecular population genetic studies have led to the concept that the unit of bacterial pathogenicity is the clone or cell line. (cdc.gov)
Distinct molecular forms of human T cell receptor gamma/delta detected on viable T cells by a monoclonal antibody. (bdbiosciences.com)
This chapter aims to explore this theme in more depth, beginning with an outline of the molecular events that dictate the differentiation of T helper (Th) cells at the outset of adaptive immune responses in regional lymph nodes. (biomedcentral.com)
There is now good evidence that there exists a coordinated programme of molecular events initiated at the outset of T-cell differentiation that leads to the generation of CD4 + Th effector cells [ 1 ]. (biomedcentral.com)
Right here, we propose a molecular system where AJC scaffolding proteins control apical cell contractility by differentially recruiting MAGI-1 and MAGI-3 to apical AZD-3965 junctions. (aboutsciencenow.info)
Used molecular Biology technique to sub-clone the gene of interest. (livecareer.com)
Molecular cloning of MDA5, phylogenetic analysis of RIG-I-like receptors (RLRs) and differential gene expression of RLRs, interferons and proinflammatory cytokines after in vitro challenge with IPNV, ISAV and SAV in the salmonid cell line TO. (uib.no)
The discovery of the molecular basis of single gene disorders , or those conditions linked to a specific genetic variant, was established when the variant that causes sickle cell disease was identified. (ashg.org)
To further characterize single gene disorders, a series of molecular techniques known as positional cloning were developed. (ashg.org)

Development of Epstein-Barr virus-specific memory T cell receptor clonotypes in acute infectious mononucleosis. (silverchair.com)
The epitopes targeted by CTLs during primary infection have not been identified, however, and there is only limited information about T cell receptor (TCR) selection. (silverchair.com)
The specificity of T-cells is determined by the T-cell receptor (TCR), a heterodimer of alpha and beta protein chains. (elifesciences.org)
Lanier LL, Ruitenberg J, Bolhuis RL, Borst J, Phillips JH, Testi R. Structural and serological heterogeneity of gamma/delta T cell antigen receptor expression in thymus and peripheral blood. (bdbiosciences.com)
The γ T-cell antigen receptor. (bdbiosciences.com)
Functional and phenotypic differences between CD4+ and CD4- T cell receptor-γ δ clones from peripheral blood. (bdbiosciences.com)
The Flt-3 ( fms- like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. (rndsystems.com)
Herein we used a single bicistronic lentiviral vector to develop a new combination immunotherapy that consists of human anti-carbonic anhydrase IX (CAIX)-targeted chimeric antigen receptor (CAR) T cells engineered to secrete human anti-programmed death ligand 1 (PD-L1) antibodies at the tumor site. (oncotarget.com)
Description: The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). (thermofisher.com)
Our previous work allowed the identification of the first positive marker for MF and SS cutaneous and circulating malignant T cells, namely the NK cell receptor KIR3DL2. (hipi-lab-saint-louis.fr)
By performing ex vivo experiments on circulating cells from Sézary patients, we established that KIR3DL2 was functional on tumor cells and acted as an inhibitory co-receptor. (hipi-lab-saint-louis.fr)

CD1c is expressed on cortical thymocytes, Langerhans cells, dendritic cells, and a subset of B cells. (biolegend.com)
that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)

Unlike dysplastic clones in myelodysplastic syndrome (MDS), which harbour intrinsic defects that predispose the cells to clonal evolution, external factors, such as pressure exerted by the immune system, have for a long time been speculated to be the main causes for clonal escape in PNH [ 5 ]. (annexpublishers.co)
Hematopoietic stem cell transplantation (bone marrow, cord blood, or peripheral blood stem cells) may cure aplastic anemia and prevent myelodysplastic syndrome or leukemia. (medscape.com)

B19 is the primary etiologic agent causing TAC in patients with chronic hemolytic anemias (e.g., sickle cell disease, hemoglobin SC disease, hereditary spherocytosis, alpha-thalassemia, and autoimmune hemolytic anemia) (22,23). (cdc.gov)

Efficient recognition of foreign pathogens by T cells requires adhesive interactions between T cells and other cell types, such as endothelial cells and antigen-presenting cells (APCs), and with components of the extracellular matrix. (scielo.br)

Studies reveal that chronic exposure of T cells to TNF uncouples TCR signal transduction pathways by impairing the assembly and stability of the TCR/CD3 complex at the cell surface. (biomedcentral.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Following activation, NK cells mediate killing of target cells through two major pathways that require direct contact between NK cells and their target cells ( 6 ). (frontiersin.org)
We discuss the rationale of various anti-fibrogenic treatment strategies targeting the clonal hematopoietic stem/progenitor cell, aberrant signaling pathways, fibrogenic cytokines, and the tumor microenvironment. (haematologica.org)
Used Western Blotting to check protein phosphorylation of different cell signaling pathways. (livecareer.com)
Overall the use of flow cytometry to analyze individual cell populations and evaluate the effects of drugs on cell function allows you "to ask the what-if questions" and to begin to integrate pathways, he said. (genengnews.com)
This method allows for multiplexed analysis of cell types and signaling pathways, and has applications in drug target identification and throughout the drug development workflow, including in the analysis of patient samples during clinical testing. (genengnews.com)
Describing the benefits of using flow cytometry to study disease specificity as it relates to cell types and signaling pathways, Dr. Kritzik reported that it is possible to measure at least 10 parameters in 5,000-10,000 single cells per second. (genengnews.com)

Using this approach, a biological sample can be analyzed in parts-exploiting the ability to separate individual cells for analysis using flow cytometry-with the data then combined to piece together the complete picture. (genengnews.com)
Peter Krutzik, Ph.D., a senior scientist in Gary Nolan's group at the Baxter Lab in genetics, pharmacology, microbiology, and immunology at Stanford University, described the use of phosphor flow cytometry to profile perturbations in signaling networks when peripheral blood cells are exposed to drug compounds. (genengnews.com)

Renal cancer occurs in a sporadic (nonhereditary) and a hereditary form, and both forms are associated with structural alterations of the short arm of chromosome 3 (3p). (medscape.com)
The CEP 12 DNA Probe Kit has been optimized only for identifying chromosome 12 in interphase nuclei from peripheral blood specimens from patients with B-cell chronic lymphocytic leukemia. (molecularcatalog.abbott)
It is not intended to be used for chromosome 12 enumeration in other patient populations or with other test matrices such as amniocytes, chorionic villi, fibroblasts, tumor cells, long term cultures, among others. (molecularcatalog.abbott)
it is not designed to detect chromosome 12 structural abnormalities. (molecularcatalog.abbott)
They described two patients with PNH with an acquired rearrangement of chromosome 12 affecting the HMGA2 gene, which encodes a chromatin remodeling protein, in the PIGA mutant cells [ 12 ]. (annexpublishers.co)
3 Myelofibrosis (MF) refers to the Philadelphia chromosome ( BCR-ABL1 )-negative myeloproliferative neoplasm (MPN) originating at the level of the multipotent hematopoietic stem cell. (haematologica.org)
Chromosome analyses showed numeral abnormalities such as 5n ploidies and various kinds of structural abnormalities, such as deletions, translocations, derivatives and isodicentric chromosomes. (biomedcentral.com)
The identified DEGs included 20 downregulated genes and 51 upregulated genes, which were highly associated with the cell cycle, organelle fission, chromosome segregation, cell division and spindle stability. (cancerindex.org)
Charcot-Marie-Tooth disease, which causes degeneration of the peripheral nerves, is an example of a CNV disorder for which a duplication of a specific region in chromosome 17 is the most common cause. (ashg.org)

Also, this recombinant protein promoted the release of NO and cell apoptosis, and inhibited the proliferation and migration of goat PBMCs and suppressed monocyte phagocytosis. (biomedcentral.com)

Much of the remainder of the discussion focuses on the different ways in which, in the longer term, the chronic inflammatory process influences maturation, differentiation, and function of effector T cells at sites of inflammation. (biomedcentral.com)
Myelophthisis is a form of bone marrow failure that results from the destruction of bone marrow precursor cells and their stroma, which nurture these cells to maturation and differentiation. (medscape.com)
Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. (thermofisher.com)
SM-AP1-3 cells were immunopositive for keratin only, indicating their duct-epithelial or squamous cell differentiation, while SM-AP4/5 cells were positive for both keratin and S-100 protein, indicating their myoepithelial cell differentiation. (biomedcentral.com)
Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)

Building of recombinant HIV-1 clones Infectious HIV-A and -C chimeric clones with V1-V3 fragments from bloodstream or cervix of the dually-infected patient had been generated as referred to previously for HIV clones 81A-4 and 49-5[20-23]. (acp2018.org)
To assess whether initial ZIKV replication in myeloid cell populations is critical for dissemination during acute infection, recombinant ZIKVs were generated that could not replicate in these specific cells. (cdc.gov)

However, the unusual phenotype of synovial T cells, including their profound proliferative hyporesponsiveness to TCR ligation, has challenged the notion that T-cell effector responses are driven by cognate cartilage antigens in inflamed synovial joints. (biomedcentral.com)
Indeed, there are now compelling data to suggest that chronic cytokine activation may contribute substantially to the phenotype and effector function of synovial T cells. (biomedcentral.com)
As an evasion mechanism, many tumors are able to stimulate the expression of immune checkpoint molecules, resulting in an exhausted phenotype of T cells that cannot restrain tumor progression [ 1 ]. (oncotarget.com)
Although malignant cells were identified as a clonal expansion of CD4+ T cell with memory phenotype, their phenotypic detection relies on the loss of pan-T cell antigens such as CD26 or/and CD7. (hipi-lab-saint-louis.fr)

A common theme demonstrated by these analyses is that distinct bacterial clones are responsible for disease outbreaks and increases in infection frequency. (cdc.gov)
One of the primary themes emerging from this research is that distinct bacterial clones have been responsible for several infection outbreaks ( Table ). (cdc.gov)
The resulting diverse naïve repertoire contains T-cell clones that recognize epitopes of yet unseen pathogens, and can participate in the immune response to infection or vaccination. (elifesciences.org)
Although overt inflammatory responses play a major role in malignant transformation of host cells following an infection, it is a disbalanced immune responses, which contribute to drive malignant transformation. (frontiersin.org)
Infection may produce a symmetrical peripheral polyarthropathy. (cdc.gov)
Whether ZIKV infection of white blood cells is required for dissemination of the virus to immune privileged sites has not been definitively shown. (cdc.gov)
Second, the authors discuss that inability to resolve infection and prevent disease may not be a consequence of the generation of an inadequate number of Th1 cells but of an intrinsic deficiency in macrophage function that prevents these cells from expressing immunity. (scielo.br)
The authors also argue that memory B and T cell responses are long-lived in the absence of antigen, but they are modulated and influenced by other infections and that memory B and T cells can either provide protective immunity or contribute to immunopathology on infection with homologous or heterologous viruses. (scielo.br)

Here we report that multiple independent somatic hematopoietic clones arise early in life, most commonly harboring heterozygous mutations in EIF6 or TP53 . (nature.com)
We show that germline SBDS deficiency establishes a fitness constraint that drives selection of somatic clones via two distinct mechanisms with different clinical consequences. (nature.com)
Somatic TP53 mutations have been observed in patients with SDS who develop MDS 13 , raising the possibility that next-generation sequencing could be integrated into surveillance for somatic clones with enhanced leukemia potential. (nature.com)
Somatic, inactivating PIGA mutations in haematopoietic stem cells, followed by an unknown autoimmune selection process in favor of the mutated clone, are thought to be important events in the pathogenesis of paroxysmal nocturnal hemoglobinuria (PNH). (annexpublishers.co)
Biochemically, PNH is a consequence of nonmalignant clonal expansion of hematopoietic stem cells with somatic mutation of PIGA [ 2 , 3 ], and mutant, inactive PIGA explains the deficiency of glycosyl phosphatidylinositol-anchored proteins [ 4 ]. (annexpublishers.co)
Risk increases with age due to the acquisition of somatic mutations that can promote clonal expansion and dominance of a particular hematopoietic stem cell, and possibly due to exposure to environmental toxins such as benzene, radiation, and chemotherapeutic agents (particularly long or intense regimens and those involving alkylating agents, hydroxyurea , and/or topoisomerase inhibitors). (msdmanuals.com)
To identify germline or somatic genomic variants associated with drug resistance of ALL cells to conventional and newer targeted anti-leukemic agents in a non-clinical, research setting. (centerwatch.com)
To compare drug sensitivity of ALL cells from diagnosis to relapse in vitro and in vivo and determine if acquired resistance to specific agents is related to specific somatic genome variants that are not detected or found in only a minor clone at initial diagnosis. (centerwatch.com)

Interestingly, the presence of a low number of PNH cells, even in healthy individuals, confirms the hypothesis that defective stem cells actually predate the stem cell failure and depletion [ 11 ]. (annexpublishers.co)
Using his expertise in global gene expression profiling, he dissected gene regulatory networks involved in the maintenance of pluripotency of embryonic stem cells. (dna-chip.co.jp)
Dissecting Oct3/4-regulated gene networks in embryonic stem cells by expression profiling. (dna-chip.co.jp)
Myelodysplastic syndromes are a group of clonal hematopoietic stem cell disorders unified by the presence of distinct mutations of hematopoietic stem cells, most frequently in genes involved in RNA splicing. (msdmanuals.com)

Moreover, the distinct clones are frequently characterized by unique combinations of virulence genes or alleles of virulence genes. (cdc.gov)
Furthermore, longitudinal repertoire analysis revealed long-term preservation of a multiclonal effector response throughout convalescence, with the reemergence of distinct memory T cell clonotypes sharing similar structural restrictions. (silverchair.com)
have recently sequenced the exome of affected cells in 12 PNH patients, and found a complex pattern of clonal evolution denoted by shared and distinct mutations in subclones, but not a single recurrent mutation besides PIGA [ 19 ]. (annexpublishers.co)
They conclude that although armed with highly distinct effector functions, there are many parallels between the T cell and B cell components of the immune response and their conversion into memory. (scielo.br)

The hierarchy of T-cell dysfunction from peripheral blood to inflamed joint suggests that these defects are acquired through prolonged exposure to proinflammatory cytokines such as tumour necrosis factor (TNF)-α. (biomedcentral.com)
The expanding number and volume of pathologic cells and the release of suppressive cytokines can eventually lead to bone marrow failure without the characteristic morphologic features of myelophthisis. (medscape.com)
Thus, the local immunological milieu in tissue compartments forms the nature and magnitude of the host responses, i.e., frequencies of regulatory T cells (Tregs) vs. T-helper 17 (Th17) cells, amount of pro-inflammatory cytokines vs. anti-inflammatory cytokines, extent of neutrophilia and antigen-presenting-cell (APC) activation, among others. (frontiersin.org)
Moreover, NK cells are poised to release cytokines such as IFN- γ , TNF-α and growth factors that can initiate inflammatory responses mediated by both the innate and the adaptive arm of the immune system. (frontiersin.org)
During the process of airway inflammation, complex interactions of innate and adaptive immune cells as well as structural cells and their cytokines have many important roles. (cdc.gov)
T helper (Th) 2 cells, which recruit and accumulate in the lungs and produce a range of different effector cytokines. (cdc.gov)
However, more recent studies have revealed the potential collaboration of other helper T cells and their cytokines in this process. (cdc.gov)
The aim of this review is to summarize the current knowledge about the possible roles of newly identified helper T cells derived cytokines (IL-9, 17, 22, 25 and IL-33) in asthma. (cdc.gov)

[ 2 ] to the presence of a few teardrop-shaped red blood cells and early myeloid precursor cells in the peripheral blood smear. (medscape.com)
Diagnosis is by blood count, peripheral smear, and bone marrow aspiration and biopsy. (msdmanuals.com)

Secondary myelofibrosis is due to implantation or invasion by malignant cancer cells that have metastasized because of implantation of blood-borne tumor cells from a distant cancer. (medscape.com)
Although bone marrow fibrosis is seen in a variety of malignant and non-malignant disease states, the deposition of reticulin and collagen fibrosis in the bone marrow of patients with myelofibrosis is believed to be mediated by the myelofibrosis hematopoietic stem/progenitor cell, contributing to an impaired microenvironment favoring malignant over normal hematopoiesis. (haematologica.org)
CD20 is a B-cell-specific antigen expressed on malignant FL cells. (spandidos-publications.com)
We mainly focus our research activity on two types of CTCL: the Mycosis Fungoides (MF), where tumor cells accumulate in the skin, and the Sézary syndrome (SS), a highly aggressive form characterized by the presence of malignant cells in the skin and blood. (hipi-lab-saint-louis.fr)
Our in vitro, ex vivo and in vivo results demonstrated that such antibody was able to promote depletion of the malignant cells by antibody-dependent cell-cytotoxicity or -phagocytosis. (hipi-lab-saint-louis.fr)
Because 10% of Sézary patients display malignant T cells that do not express KIR3DL2, we pursue our search for specific tumor antigens. (hipi-lab-saint-louis.fr)
We recently reported that CD39, an ectonuclotidase involved in the process of generating adenosine from ATP, is aberrantly expressed by Sézary patients' malignant cells. (hipi-lab-saint-louis.fr)
It is still poorly understood how and which benign pleomorphic adenoma cells transform into its malignant form, carcinoma ex pleomorphic adenoma. (biomedcentral.com)
Although pathologists in their daily services of surgical pathology had recognized such singular atypical cells in pleomorphic adenomas, these atypical cells were not always regarded as evidence or the source for malignant transformation [ 4 - 6 ]. (biomedcentral.com)

For cytokine genes, at least three stages are thought to be required for the acquisition of transcriptional competence in T cells: an initiation phase, a commitment phase, and a phase of acute gene transcription (Fig. 1 ). (biomedcentral.com)
Recently, a second clonal event involving the HMGA2 gene was reported in some PNH cases, and over expression of this gene was postulated to promote proliferation of the mutated clone. (annexpublishers.co)
Selected mutations were introduced into a prM-E gene cassette and expressed transiently in COS cells to investigate whether the mutations impaired prM/E association or secretion. (microbiologyresearch.org)

Periodically 'cleansed' of spontaneous mutants: Prior to dose-range finding and mutagenicity testing, the mouse lymphoma cells were grown for 1 day in R10-medium containing hypoxanthine, aminopterine and thymidine to reduce the amount of spontaneous mutants, followed by a recovery period of 2 days on R10-medium containing hypoxanthine and thymidine only. (europa.eu)
Our team has expertise in skin cancers, with a special interest in cutaneous T cell lymphoma, melanoma and carcinoma. (hipi-lab-saint-louis.fr)
Our team develops research projects dedicated to skin cancers with a main interest on melanoma, cutaneous T cell lymphoma (CTCL) and carcinoma. (hipi-lab-saint-louis.fr)
Mycosis Fungoides and Sézary syndrome are two forms of cutaneous T cell lymphoma associated to a poor prognosis at advanced stages due to a lack of curative treatments. (hipi-lab-saint-louis.fr)
Cutaneous T cell lymphoma (CTCL) is a heterogeneous group of non-Hodgkin lymphomas that originate from the skin. (hipi-lab-saint-louis.fr)
and one follicular mixed small cleaved and large cell (fm) lymphoma according to the working formulation) are presented. (liverpool.ac.uk)
Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)

Go to Clear Cell Renal Cell Carcinoma and Sarcomatoid and Rhabdoid Renal Cell Carcinoma for complete information on these topics. (medscape.com)
One of the new emerging mechanisms associated with the progression of clear cell renal cell carcinoma (ccRCC) and other tumors is the immune checkpoint pathway, which consists of cellular interactions that prevent excessive activation of T cells under normal conditions. (oncotarget.com)

MTT colorimetric assays, colony formation assays and 5‑ethynyl‑20‑deoxyuridine incorporation assays were also conducted to evaluate ACC cell proliferation. (cancerindex.org)

Therefore, a central goal in clinical care of SDS patients is to identify incipient leukemic transformation and initiate pre-emptive treatment with allogeneic stem cell transplantation. (nature.com)
Experimental treatment approaches include vaccines and nonmyeloablative allogeneic peripheral blood stem cell transplantation. (medscape.com)

Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. (rndsystems.com)
Transcriptomic profiling of T-cell populations in non-muscle invasive and muscle invasive bladder cancer. (lu.se)
Automation of the entire process-from sample preparation, to running complex assays, to data analysis-makes it possible to perform functional phenotypic profiling of cell populations efficiently. (genengnews.com)
This capability is particularly important when studying heterogeneous cell populations and a variety of different phosphoproteins. (genengnews.com)
The researchers were able to correlate cell populations with pathway activity. (genengnews.com)
To assess clonal diversity and evolution of pre-leukemic and leukemic populations using DNA variant detection and single-cell genomic analyses in a non-clinical, research setting. (centerwatch.com)

Many of these clones are characterized by unique combinations of virulence genes or alleles of virulence genes. (cdc.gov)
Genetic studies of the families at high risk for developing renal cancer led to the cloning of genes whose alteration results in tumor formation. (medscape.com)
Expression of cloned envelope protein genes from the flavivirus tick-borne encephalitis virus in mammalian cells and random mutagenesis by PCR. (microbiologyresearch.org)
We have identified many other causes of genetic anomalies, including single nucleotide variants and larger structural changes that alter protein-encoding genes. (ashg.org)

Plasmalemmal Cl - -HCO3 - exchangers regulate intracellular pH and [Cl - ] and cell volume. (tcdb.org)
a technique of derivatization of proline (pro) and 4-hydroxyproline (hyp) by 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole permitted the measurement of pro and hyp radioactivities, concentrations, and specific activities in the main fractions separated from cultures of fibroblast cells (extracellular collagen and non-collagen proteins, intracellular free pro and hyp, pro- and hyp-containing peptides, procollagen, and non-collagen proteins). (liverpool.ac.uk)

T-cell clones were then tested for specificity with amoxicillin, unmodified- and amoxicillin-modified peptides, and structural variants. (liverpool.ac.uk)

PNH clones are present in a majority of patients with aplastic anemia, which is consistent with the strong immune component in the pathogenesis of this disease [ 7-9 ]. (annexpublishers.co)

The mechanisms of action by which RTX kills FL cells include complement-dependent cytotoxicity (CDC) ( 3 ), antibody-dependent cellular cytotoxicity (ADCC) ( 4 , 5 )-herein referred to as 'effector function' mechanisms-as well as CD20 signaling effects ( 6 ). (spandidos-publications.com)

At the time of disease progression, the same chromosomal abnormalities were present in the bone marrow, peripheral blood and the EM lesion: del(13)(q14), del(17)(p13), t(4;14)(p16;q32) and gain(1)(q21). (muni.cz)
Extramedullary lesion most probably originated by an expansion of one clone of tumor plasma cells from the bone marrow, which was confirmed by identical genomic profile of both tested samples. (muni.cz)
Current surveillance strategies for patients with SDS and other leukemia predisposition syndromes rely on monitoring hematologic status by serial peripheral blood counts to identify worsening cytopenias and bone marrow examinations to identify morphologic changes or development of clonal chromosomal abnormalities 11 . (nature.com)
Infiltrating lesions caused by nonhematopoietic cells invading bone marrow can result in varying degrees of cytopenia, including anemia , thrombocytopenia, neutropenia, and pancytopenia. (medscape.com)
[ 1 ] Bone marrow failure resulting from secondary infiltration is a possible cause of lack of blood cell production (as differentiated from a primary cause of failure). (medscape.com)
Bone marrow cells are completely replaced by large carcinoma cells with clear nucleoli. (medscape.com)
The bone marrow becomes infiltrated by collagen, reticulin, and other forms of fibrosis, which replace the normal, hematopoietic cells. (medscape.com)
The most common causes of extensive bone marrow infiltrative damage or invasion without much structural damage are discussed below. (medscape.com)
Leukemic cells, such as those occurring in chronic leukemias in which the expanding cells are mature and coexist peacefully with the normal bone marrow cells, show no evidence of myelophthisis, and marrow damage does not occur. (medscape.com)
Hematopoietic stem cell transplantation remains the only curative therapeutic approach that reliably results in resolution of bone marrow fibrosis in patients with myelofibrosis. (haematologica.org)
Bone marrow-derived cells were prepared by flushing femurs and tibiae with sterile RPMI 1640 supplemented with 10% heat-inactivated FCS, as previously described [22]. (ainhibitor.com)
To evaluate and implement deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) sequencing-based methods to monitor levels of MRD in bone marrow, blood, and cerebrospinal fluid. (centerwatch.com)

To determine in a randomized study design whether the incidence and/or severity of acute vincristine-induced peripheral neuropathy can be reduced by decreasing the dosage of vincristine in patients with the high-risk CEP72 TT genotype or by shortening the duration of vincristine therapy in standard/high-risk patients with the CEP72 CC or CT genotype. (centerwatch.com)

Deletions of 3p occur commonly in renal cell carcinoma associated with von Hippel-Lindau disease. (medscape.com)

Therefore whilst CR3022 can neutralise SARS-COV in in a concerted action with clone CR3014, CR3022 is not believed to independently neutralise SARS-COV2, based on in vitro studies (32226289, 32065055, 32383254, 32416259). (abcam.com)
Anti-TCR-γ/δ is intended for in vitro diagnostic use in the identification of cells expressing TCR-γ/δ antigen, using a BD FACS™ brand flow cytometer. (bdbiosciences.com)
This study demonstrates in vitro that certain cell types from pleomorphic adenoma are able to clone and survive over a long term and develop subcutaneous tumors in nude mice. (biomedcentral.com)

In contrast, knockdown of BRD4 but not of BRD2 impairs NK cell cytolytic responses, suggesting BRD4 as critical regulator of NK cell mediated tumor cell elimination. (frontiersin.org)
This is supported by pharmacological targeting where the first-generation pan-BET bromodomain inhibitor JQ1(+) displays anti-inflammatory effects and inhibit tumor cell eradication, while the novel bivalent BET bromodomain inhibitor AZD5153, which shows differential activity towards BET family members, does not. (frontiersin.org)
The present study demonstrated that CA125 could bind to RTX and reduce its tumor cell killing activity. (spandidos-publications.com)
Our main goal is to identify new tumor antigens specifically expressed at the tumor cell surface and to validate their potential use as new therapeutic targets through the development of antibody-based immune-therapies. (hipi-lab-saint-louis.fr)
Notably, the pathophysiology of these diseases is still controversial especially regarding the tumor cell origin or the tumorigenic triggering factors. (hipi-lab-saint-louis.fr)

The expression of PD-L1 and Ki67 in the tumors decreased and an increase in granzyme B levels was found in CAR T cells. (oncotarget.com)

Functional characterization of IgM+ B cells and adaptive immunity in lumpfish (Cyclopterus lumpus L.). Developmental and Comparative Immunology. (uib.no)

The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. (rndsystems.com)
Endothelial cell senescence or activation is dependent on the type of cytokine, ligand concentration, cell-cell interaction, MK 1775 and a multitude of other signaling molecules. (ainhibitor.com)

This blood film at 1000X magnification demonstrates a leukoerythroblastic blood picture with the presence of precursor cells of the myeloid and erythroid lineage. (medscape.com)
MF is characterized by variable degrees of cytopenias, a leukoerythroblastic blood picture, and extramedullary hematopoiesis resulting in progressive splenomegaly and debilitating disease-related constitutional symptoms, compromising quality of life. (haematologica.org)

Amoxicillin-modified KP-2 and KP-3 peptide-specific CD4+ clones proliferated and secreted interferon gamma (IFN-γ), interleukin (IL)-10, perforin and/or IL-17/IL-22 in a dose-dependent manner and displayed no cross-reactivity with amoxicillin, unmodified peptide or with positional derivatives. (liverpool.ac.uk)

TCR-beta sequence analysis of CTL clones specific for each epitope showed polyclonal TCR-beta repertoire selection, with structural restrictions on recognition that indicated antigen-driven selection. (silverchair.com)
Structural modelling has confirmed that CR3022 targets a conserved epitope between SARS-CoV and SARS-CoV2 in the RBD domain (32245784, 32065055). (abcam.com)
The binding epitope of clone CR3022 does not overlap with the ACE2 binding site of SARS-COV2 (32065055). (abcam.com)
Estimating the fraction of the T-cell response directed against the single immunodominant epitope, we identify the sequence features of TCRs that define the high precursor frequency of the two major TCR motifs specific for this particular epitope. (elifesciences.org)
Mutations in the CT have been shown to affect the conformation of gp120 ectodomain [ 16 - 18 ], and more recently, Durham et al suggested that the CT regulates the conformation of Env at the cell surface and control epitope exposure through T cell virological synapses [ 19 ]. (plos.org)

However, the involvement of this protein in parasite pathogenesis remains elusive and its effect on the functions of innate immune cells is unknown. (biomedcentral.com)
Because of its involvement in F. gigantica recognition by innate immune cells, rFg14-3-3e protein may have applications for development of diagnostics and therapeutic interventions. (biomedcentral.com)

EIF6 inactivation mediates a compensatory pathway with limited leukemic potential by ameliorating the underlying SDS ribosome defect and enhancing clone fitness. (nature.com)

Results Lack of ZO proteins highly perturbs Par-3 localization and alters apical morphology We previously demonstrated that depletion of ZO proteins in the mouse mammary epithelial cell range, EpH4, delays the forming of the contractile belt-like AJ18, recommending that ZO proteins are necessary for epithelial polarization. (aboutsciencenow.info)

Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. (thermofisher.com)

Tissue, cells or virus corresponding to Human coronavirus SARS-CoV-2 Spike Glycoprotein S1. (abcam.com)
Mouse Flt-3 has also been cloned and shown to share 85% amino acid sequence identity with human Flt-3. (rndsystems.com)
The anti-PD-L1 IgG1 isotype, which is capable of mediating ADCC, was also able to recruit human NK cells to the tumor site in vivo . (oncotarget.com)
These armed second-generation CAR T cells empowered to secrete human anti-PD-L1 antibodies in the ccRCC milieu to combat T cell exhaustion is an innovation in this field that should provide renewed potential for CAR T cell immunotherapy of solid tumors where limited efficacy is currently seen. (oncotarget.com)
Staining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0038-42) and 0.25 µg of Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (left) or Anti-Human CD8a eFluor® 450 (right). (thermofisher.com)
Applications Tested: This RPA-T8 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
The development of the human blood-CSF-brain barrier. (cdc.gov)
the disease caused by the cognate cel s from human cord blood. (who.int)

The diverse repertoire of T-cell receptors (TCR) plays a key role in the adaptive immune response to infections. (elifesciences.org)

However, the results of detailed phenotypic and functional analyses of chronically activated T cells derived from inflamed joints are difficult to reconcile with traditional models of cartilage-antigen-driven inflammatory disease in patients with RA. (biomedcentral.com)

little attention has been devoted to the mechanisms and other immune cells. (hindawi.com)

the CEP 12 DNA Probe Kit has been optimized for archived peripheral blood specimens stored only at - 20°C or - 80°C, as recommended. (molecularcatalog.abbott)
B19 was discovered in England in 1975 in serum specimens from healthy blood donors (1). (cdc.gov)

Generally, in myelophthisic anemia, a form of fibrosis, occurs secondary to injury by nonhematopoietic cells or pathogens. (medscape.com)
Monitored secondary and tertiary structural changes of the protein as a function of denaturant using circular dichroism (CD) and Tryptophan. (livecareer.com)
In the B cell system protective memory is mediated by plasma cells that secrete antibodies, whereas reactive memory is mediated by memory B cells that proliferate and differentiate to plasma cells in response to secondary antigenic stimulation. (scielo.br)
The histological features of squamous cell carcinoma from the transplanted cell systems in nude mice might suggest a secondary onset of malignancy from a pre-existing benign adenoma. (biomedcentral.com)
Symptoms are referable to the specific cell line most affected and may include fatigue, weakness, pallor (secondary to anemia), increased infections and fever (secondary to neutropenia), and increased bleeding and bruising (secondary to thrombocytopenia). (msdmanuals.com)

T-cells play a crucial role in the immune response to pathogens by mediating antibody formation and clearance of infected cells, and by defining an overall response strategy. (elifesciences.org)

Drug-specific CD4+ T cells have been detected in patients with DILI, suggestive of an immune etiology. (liverpool.ac.uk)

2007. Leukocyte and Stromal Cell Molecules:The CD Markers. (biolegend.com)

Mutations encoding basic (Arg), non-polar (Leu and Pro) and uncharged polar (Asn, Gln and Tyr) amino acids at M39 were introduced into a DENV-2 genomic-length cDNA clone and their effects on virus replication were examined. (microbiologyresearch.org)

Interleukin (IL)-9-producing subset called Th9 cell, Th22 cells which primarily secrete IL-22, IL-13 and tumor necrosis factor- and Th25 cells via producing IL-25 are believed to be important for initiating allergic reactions and developing airway inflammation. (cdc.gov)

Reliable studies, showing negative results with and without metabolic activation, are available on genetic toxicity in bacteria and mammalian cells. (europa.eu)

Anatomy48

Organisms11

Diseases26

Chemicals and Drugs77

Analytical, Diagnostic and Therapeutic Techniques and Equipment51

Phenomena and Processes51

Disciplines and Occupations1

Information Science4

Health Care4

Mononuclear cells2

Lymphocytes13

Monocytes8

Epithelial cells5

Viral13

Subsets5

Molecular9

Receptor11

Dendritic cells2

Myelodysplastic syndrome2

Sickle cell di1

Endothelial cells1

Pathways8

Flow Cytometry2

Chromosome9

Apoptosis1

Differentiation5

Recombinant2

Phenotype4

Infection8

Somatic8

Stem cells4

Distinct4

Cytokines8

Smear2

Malignant9

Gene3

Lymphoma7

Clear Cell Renal Cell Carc2

Proliferation1

Allogeneic2

Populations6

Genes4

Intracellular2

Specificity1

Aplastic anemia1

Antibody-dependent ce1

Bone12

Acute1

Deletions1

Vitro3

Tumor cell5

Expression1

Characterization1

Ligand2

Leukoerythroblastic blood picture2

Positional1

Epitope5

Innate2

Pathway1

Depletion1

Antigen-prese1

Human8

Adaptive1

Analyses1

Immune cells1

Specimens2

Secondary5

Pathogens1

Etiology1

Leukocyte1

Mutations1

Subset1

Mammalian cells1