Cells in organ culture. Medical search. Frequent questions

The development of emerging in-vitro tissue culture platforms can be useful for predicting the human response to new compounds . (fluigent.com)
Recently, several in-vitro tissue-like microsystems, also known as " organ-on-a-chip studies", have emerged to provide new tools for better evaluating the effects of various chemicals on human tissue . (fluigent.com)
In biological research and biotechnology callus formation is induced from plant tissue samples (explants) after surface sterilization and plating onto tissue culture medium in vitro (in a closed culture vessel such as a Petri dish). (wikipedia.org)
In vitro experiments using NV virus-like particles (VLPs) directly showed NV VLP attachment to HBGA, resulting in VLP internalization into the cell ( 19 ). (cdc.gov)
From these critical studies, putative NV receptors were identified, and thus it was hypothesized that a successful in vitro cell culture system would most likely possess these receptors to support NV replication. (cdc.gov)
Thymic atrophy was the result of direct action of THC and not based on maternal factors inasmuch as THC was able to induce T cell apoptosis in vitro in fetal thymic organ cultures. (aspetjournals.org)
4) By culturing the first node in the SLCE system, we performed a quick survey during the 3 weeks in the culture and found that a combination of 10 μM benzyl adenine and 3 μM thidiazuron was effective for in vitro SAM development, while the addition of 2, 4-D was effective for promoting in vitro RAM development. (scirp.org)
2008) specifically regarding in vitro germination of the caryopses and plant regeneration from nodal segments of both the germinated seedlings and tissue-cultured clone plants [4]. (scirp.org)
In vitro selection of salt tolerant cell lines in Solanum tuberosum L . Biologia Plantarum, 51 (4), 728 - 734. (up.pt)
In vitro organ culture models provide a useful method for examining how specific loading patterns affect the cellular response to load which may explain the early mechanisms of tissue injury associated with tendinopathies and ligament injuries. (cdc.gov)
Experimental pharmacological methods are introduced, including operation techniques and in vitro experiments with excised tissues or organs. (lu.se)

The tissues used to initiate callus formation depends on plant species and which tissues are available for explant culture. (wikipedia.org)
The callus tissues then undergo further cell growth and differentiation, forming the respective organ primordia. (wikipedia.org)
Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
Gottlieb Haberlandt first pointed out the possibilities of the culture of isolated tissues, plant tissue culture . (wikipedia.org)
[12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
Triggering specific chemical reactions in the disease microenvironment can produce species for disease treatment that have high theranostic performance and low side effects on healthy cells/tissues. (rsc.org)
The system can be used to examine the relationship between each loading parameter and biomechanical factors of connective tissues maintained in culture which may provide useful information regarding the etiology of overuse injuries. (cdc.gov)
Although previous recommendations for preventing transmission of human immunodeficiency virus (HIV) through transplantation of human tissue and organs have markedly reduced the risk for this type of transmission, a case of HIV transmission from a screened, antibody-negative donor to several recipients raised questions about the need for additional federal oversight of transplantation of organs and tissues. (cdc.gov)
A working group formed by the Public Health Service (PHS) in 1991 to address these issues concluded that further recommendations should be made to reduce the already low risk of HIV transmission by transplantation of organs and tissues. (cdc.gov)
In 1985, when tests for HIV antibody became available, screening prospective donors of blood, organs, and other tissues also began (2,3). (cdc.gov)
This occurrence raised questions about the need for additional federal oversight of transplantation of organs and tissues. (cdc.gov)
The working group concluded that, although existing recommendations are largely sufficient, revisions should be made to reduce the already low risk of HIV transmission via transplantation of organs and tissues. (cdc.gov)

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Paracoccidioidomycosis is progressive mycosis of the lungs, skin, mucous membranes, lymph nodes, and internal organs caused by Paracoccidioides brasiliensis . (msdmanuals.com)

Since rodent models cannot recapitulate many of the human disease features, human induced pluripotent stem cells derived from Parkinson's patients have been used to generate brain organoids, greatly contributing to our understanding of the disease pathophysiology. (nature.com)
To understand the multifaced aspects of Parkinson's disease, it may be desirable to expand the complexity of these models, to include different brain regions, vasculature, immune cells as well as additional diverse organ-specific organoids such as gut and intestine. (nature.com)
The authors said the organotypic model accurately recapitulates appendiceal tumors in terms of cell mix and behavior, which makes it a promising new research tool in addition to other approaches, such as organoids and patient-derived xenografts (transplanted tissue grown in another species, such as a mouse or rat). (ucsd.edu)
For instance, the organ-on-a-chip paradigm - the development of organoids on electronic chips - allows scientists to assay cultures of cardiac cells for electrical activity in real time. (corning.com)
That is according to a study in human cell cultures and organoids by researchers at Karolinska Institutet in Sweden and the University of British Columbia (UBC) in Canada, published in the journal Cell. (sciencebusiness.net)
The authors were also able to verify these data from regular cell cultures in engineered miniature replicas of blood vessels and kidneys, so-called organoids grown from human stem cells. (sciencebusiness.net)
In addition recent development of sophisticated organoids (a simplified 3D version of a corresponding in vivo organ, which can be cultured in lab) will allow us to rely less on animal models and 2D cell cultures (that don't always perfectly recapitulate the cancer biology in patients). (lu.se)

We transplanted 30 donor corneas preserved at 34 C for 15 to 33 days in culture medium containing 1.35% chondroitin sulfate and compared them with 30 corneas transplanted during the same period, but preserved only in McCarey-Kaufman medium at 4 C for one to 81 hours. (nih.gov)
These results demonstrated that donor corneal endothelial cells preserved for up to one month at 34 C in 1.35% chondroitin sulfate appear to survive and function after keratoplasty as well as those preserved at 4 C for one to three days in McCarey-Kaufman medium. (nih.gov)
By using a patient's own stem cells it could help to reduce the risk of the transplanted organ being rejected while also providing a plentiful supply of donor organs. (cbc-network.org)
The abnormal wound healing is due to impaired limbal epithelial stem cells, and the new research, published online July 18 in Diabetologia , involved isolation of those cells from 30 donor eyes of humans with and 23 without diabetes. (medscape.com)
A 1991 investigation determined that several recipients had been infected with HIV by an organ/tissue donor who had tested negative for HIV antibody at the time of donation (4). (cdc.gov)

One group of these proteins is called immunoglobulins, or antibodies, that are produced by specialized cells called B cells. (sciencedaily.com)
Now, new lifecycle analysis from the Good Food Institute, a non-profit group that is working to support the development of alternative proteins including cultured meat, claims the production cost of cell-based meat when manufactured at scale could drop to €4.68 per kg, thus matching the price of its conventional counterparts, as soon as 2030. (foodnavigator.com)
Briefly, cells that are pre-labeled with fluorescence dye, antibodies conjugated to fluorophores, or expressing fluorescence proteins, are loaded into a stream. (bgu.ac.il)
Epithelial cells adjacent to the area of the defect flatten, lose their hemidesmosome attachments, and migrate on transient focal contact zones that are formed between cytoplasmic actin filaments and extracellular matrix proteins. (medscape.com)

However, the molecular mechanisms which regulate Th1 cell differentiation in CD mucosa are unknown. (bmj.com)
Various subsets of invariant natural killer T (iNKT) cells with different cytokine productions develop in the mouse thymus, but the factors driving their differentiation remain unclear. (nih.gov)
Our data therefore suggest that TCR signal strength during thymic differentiation may influence not only IFN-γ production, but also the protective function of iNKT cells in arthritis. (nih.gov)

To study the production of IgA within the lungs, the researchers used mouse models to isolate specialized cells called dendritic cells and cultured them with antibody-producing B cells. (sciencedaily.com)
A drug already tested against lung disease could potentially inhibit COVID-19 by reducing the coronavirus load that enters the lungs and other organs. (sciencebusiness.net)
It distracts the virus from infecting the cells to the same degree and should lead to a reduction in the growth of the virus in the lungs and other organs. (sciencebusiness.net)
ACE2 usually helps lungs and other organs to maintain normal function but when viruses bind to the protein they could damage the cells. (sciencebusiness.net)
Examples include organ transplants and iron lungs. (cdc.gov)

Molecular biological methods, cell culture and tissue culture are treated to a less extent. (lu.se)

FUT2 encodes an enzyme that produces histo-blood group antigens (HBGA) on the surface of epithelial cells and in mucosal secretions ( 21 , 22 ). (cdc.gov)
Metalloproteinases are secreted as proenzymes by neutrophils infiltrating the wound, injured epithelial cells, and keratocytes. (medscape.com)

Lamina propria T lymphocytes (T-LPL) isolated from the intestine of patients with active CD released interferon (IFN)-γ in response to gluten stimulation, and neutralisation of endogenous IFN-γ prevented the gliadin mediated morphological changes in an ex vivo organ culture of treated CD biopsies. (bmj.com)
We conclude that BLI is a promising tool to monitor spatial and temporal cellular behavior in ex vivo organ culture. (amsterdamumc.org)
Hence, ex vivo organ culture systems allow pre-screening and pre-validation of novel therapeutic concepts prior to in vivo large animal experimentation. (amsterdamumc.org)

On the first postoperative day, the organ-cultured grafts were thicker than those grafts preserved in McCarey-Kaufman medium, and the thickest corneas were those cultured for the longest times. (nih.gov)
01), and improved wound healing by 30% in diabetic organ-cultured corneas. (medscape.com)

The researchers injected stem cells from rats into the embryos of mice that had been genetically altered so they could not produce their own organs, creating mice that had rat organs. (cbc-network.org)
The researchers say the technique could allow pigs to grow human organs from patient's stem cells for use as transplants. (cbc-network.org)
It meets the needs of beginners in organ-on-chip cell culture research and advanced organ-on-chip researchers looking for automation and reproducibility. (fluigent.com)
Regeneration of a whole plant from a single cell allows transgenics researchers to obtain whole plants which have a copy of the transgene in every cell. (wikipedia.org)
In a new study published in the journal Scientific Reports , researchers from New York University's School of Medicine say they have found a new organ they're calling the "interstitium. (nationalgeographic.com)
Now researchers report that microbiota regulate the ability of lung dendritic cells to generate immune responses. (sciencedaily.com)
Microbiota--the trillions of bacteria that co-exist in the body--regulate the ability of lung dendritic cells to generate immune responses, according to a study led by researchers from the Icahn School of Medicine at Mount Sinai, published online in the Journal of Experimental Medicine . (sciencedaily.com)
To the researchers' surprise, they found that when they isolated lung dendritic cells from "germ free" or antibiotic-treated mice, the production of IgA was highly impaired. (sciencedaily.com)
In the study now published in Cell , the researchers used tissue samples from a patient with COVID-19 to isolate and cultivate SARS-CoV-2, the virus that causes the disease COVID-19. (sciencebusiness.net)
It is the same mechanism that the original SARS-virus from 2003 used to bind to our cells, and which has been described by several of the researchers in previous studies. (sciencebusiness.net)
By adding a genetically modified variant of this protein, called human recombinant soluble angiotensin-converting enzyme 2 (hrsACE2), the researchers wanted to test if the virus could be stopped from infecting the cells. (sciencebusiness.net)
This could, according to the researchers, explain why some COVID-19 patients suffer severe lung disease and multi-organ failure. (sciencebusiness.net)

In 2005, he wrote an essay in Nature challenging the importance of "cell theory"-a concept that holds cells as the basic structure of all organisms, and in 2001, he published a paper finding that adult stem cells could be made to act similarly to embryonic cells. (nationalgeographic.com)

Callus cells are not necessarily genetically homogeneous because a callus is often made from structural tissue, not individual cells. (wikipedia.org)
Regeneration of a whole plant that has some genetically transformed cells and some untransformed cells yields a chimera. (wikipedia.org)
The lifespan of most cells is genetically determined, but some cell-culturing cells have been "transformed" into immortal cells which will reproduce indefinitely if the optimal conditions are provided. (wikipedia.org)

One ultimate goal is for 3D culture to drive synthetic tissue and organ growth for transplants. (corning.com)

Scientists have found they can create chimeric animals that have organs belonging to another species by injecting stem cells into the embryo of another species. (cbc-network.org)
More importantly, there is a clear upward trend in studies that utilize human-induced pluripotent stem cells (hiPSC) to develop personalized tissue or organ models . (fluigent.com)
Goat adipose-derived stem cells (gADSCs) were transduced with either Firefly luciferase (Fluc) or Gaussia luciferase (Gluc) reporter genes and injected in isolated goat intervertebral discs (IVD). (amsterdamumc.org)
However, treatment with various approaches, including exogenous WNT5A methylation inhibitors and a nanoconjugate that inhibits WNT5A suppression, improved corneal epithelial wound healing as well as expression of the limbic epithelial stem cells. (medscape.com)
Overall, Wnt-5a is a new corneal epithelial wound healing stimulator that can be targeted to improve wound healing and stem cells in the diabetic cornea," write Ruchi Shah, PhD, of the Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, California, and colleagues. (medscape.com)
The finding of Wnt-5a as a new diabetic corneal marker regulating wound healing and stem cell function may have implications for other diabetes complications involving impaired wound healing, including diabetic foot ulcers, as they share similar neurovascular, sensory, and immunological compromise with diabetic eye disease, Shah and colleagues say. (medscape.com)
On the other hand, the market is also divided by application, including cancer studies and stem cell research. (corning.com)
But fields such as regenerative medicine and stem cell research could grow as the market matures in the coming years, as they represent the majority of the overall upswing in research spending. (corning.com)
One of the most promising avenues for 3D cell culture technology is the use of organoid models , synthetic environments that grow small tissue samples using stem cells. (corning.com)
Organoid models could also enable doctors to grow a wide variety of cell types from harvested stem cells, making the dream of truly personalized medicine much more achievable. (corning.com)
Basal and limbal stem cells contribute to mitosis. (medscape.com)
A sufficient supply of progenitor stem cells to facilitate epithelial cell proliferation is important for the cornea. (medscape.com)
A deficiency of limbal stem cells, from either disease (eg, aniridia) or trauma (eg, chemical burn), can preclude adequate epithelial wound healing. (medscape.com)

These biomimetic platforms overcome many of the drawbacks encountered with conventional tissue culture models. (fluigent.com)
For example, studies have speculated on the potential health benefits and drawbacks of cultured meat. (foodnavigator.com)

Peripheral T cells from such mice showed decreased proliferative response to T cell mitogen as well as both T cell and antibody response to HIV-1 p17/p24/gp120 antigens. (aspetjournals.org)
Further, when the germ-free mice were fed with a component of bacterial cell wall called LPS, the lung dendritic cells started instructing B cells to produce IgA again. (sciencedaily.com)

In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph . (wikipedia.org)
At 7wk, HF-fed animals exhibited several immune alterations (blood leukocyte/neutrophil number, lymph node B-cell proportionality)-effects which were more pronounced in SD rats. (cdc.gov)

In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
ABSTRACT To determine the microbiology of wound infection following caesarean section and to evaluate the use of Gram stain for the predicton of subsequent microbiological culture results, 1319 surgical wounds were followed up. (who.int)

Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture . (wikipedia.org)

According to Reuters' Market Study Report, income from 3D cell culture products could almost double in the next five years, from $510 million in 2019 to a projected $970 million in 2024. (corning.com)

In alfalfa, Medicago truncatula, however callus and somatic embryos are derived from mesophyll cells that undergo dedifferentiation. (wikipedia.org)
2005). Effect of hydrogen peroxide on catalase gene expression, isoform activities and levels in leaves of potato sprayed with homobrassinolide and ultrastructural changes in mesophyll cells . (up.pt)

Organisms seen by Gram stain yielded a sensitivity of 96.6%, specificity of 88.9%, positive predictive value of 97.7% and negative predictive value of 84.2% when used to predict positive culture results for bacterial wound infection. (who.int)
logic recovery of pathogenic organisms by Purulent exudates were obtained from culture. (who.int)
Culturing was done within 1 hour showed predominantly white blood cells using standard bacteriological inoculation and no organisms [ 6,7 ]. (who.int)

Two months after keratoplasty there was no statistically significant difference in central endothelial cell loss between the organ-cultured grafts and those preserved in McCarey-Kaufman medium (9% vs 7% cell loss, respectively). (nih.gov)

Several studies have clearly demonstrated that distinct cytokine activated signalling and transcription factors regulate the commitment of a naïve T cell along the Th1 or T helper cell type 2 (Th2) phenotype, as well as maintenance of the polarised phenotype. (bmj.com)

Microfluidics provides the unique ability to control the cellular microenvironment with high spatiotemporal precision and to present cells with mechanical and biochemical signals in a more physiologically relevant context . (fluigent.com)
Integrating ECM materials in microfluidics for 3D cell culture. (rsc.org)

2) Since the obtained shoots of a 1-month-old culture box showed growth variation, we distinguished shoot types based on plant height, i . e ., short (less than 5 cm), medium (ca. 5 - 10 cm), and tall (more than 10 cm). (scirp.org)
After 4 weeks, shoots were reversed from cultured cyathiums. (ncl.edu.tw)

1990. Subcommittee report on biological indicators of organ damage. (cdc.gov)
Fluorescence-Activated Cell Sorting (FACS) is a method for analysis and sorting of cells and other biological particles (e.g. exosomes) based on light scattering and fluorescence characterizations. (bgu.ac.il)

1- 5 Moreover, we have previously shown that the Th1 cell response in fetal gut explants results in villous atrophy and crypt cell hyperplasia, 6, 7 thus supporting the role of local Th1 cell inflammation in promoting CD associated tissue damage. (bmj.com)

Moreover, perinatal exposure to Δ 9 -tetrahydrocannabinol (THC) had a profound effect on the fetus as evidenced by a decrease in thymic cellularity on gestational days 16, 17, and 18 and postgestational day 1 and marked alterations in T cell subpopulations. (aspetjournals.org)

The motor symptoms are attributed to dopaminergic cell loss within the substantia nigra (SN) pars compacta, resulting in subsequent dysfunction of the basal ganglia, a cluster of deep nuclei that participate in the initiation and execution of movements [ 3 ]. (nature.com)
There are several types of basal salt mixtures used in plant tissue culture, but most notably modified Murashige and Skoog medium, White's medium, and woody plant medium. (wikipedia.org)
The phrase lives of a cell refers to the independent yet interrelated parts of a human cell including mitochondria, centrioles, and basal bodies that once led independent lives. (cdc.gov)

Again, we see the importance of animal research, with the potential that the organ shortage could be largely solved by growing patient DNA-identical human organs in pigs for transplant. (cbc-network.org)
If we can eat bacon, we can use pigs to grow organs for human use. (cbc-network.org)
Organ-on-a-chip (OOAC) is the concept of mimicking the organ-level function of human physiology or disease using cells inside a microfluidic chip . (fluigent.com)
Organ-on-chip cell culture models can therefore be used for accurate prediction and mechanistic investigation of dose-limiting human toxicities of prospective drugs , as well as for the exploration of new therapeutic approaches to mitigate the observed toxic effects. (fluigent.com)
About 70 percent of the human body is made of water, and about two thirds of that is found in cells. (nationalgeographic.com)
Recently, ex vivo three-dimensional organ culture systems have emerged to study the physiology and pathophysiology of human organs. (amsterdamumc.org)
The human body contains approximately ten times as many bacterial cells as human cells. (sciencedaily.com)
MATERIALS AND METHODS: Human osteosarcoma cell lines U2OS, SaOS2, MNNG/HOS (HOS) and 143B, were used. (bvsalud.org)
Our human lives do not depend just on the lives in our individual cells. (cdc.gov)

SASSONE-CORSI, P. Light acts directly on organs and cells in culture to set the vertebrate circadian clock. (bvsalud.org)

Viral culture is also related, with cells as hosts for the viruses. (wikipedia.org)
The result now published shows that hrsACE2 reduced viral growth of SARS-CoV-2 by a factor of 1,000 to 5,000 in cell cultures. (sciencebusiness.net)

The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth. (helsinki.fi)
2006). Phylogenetic relationship of potato CAT1 and CAT2 genes, their differential expression in non-photosynthetic organs and during leaf development, and their association with different cellular processes . (up.pt)

The target of Al toxicity is the root tip, in which Al exposure causes inhibition of cell elongation and cell division, leading to root stunting accompanied by reduced water and nutrient uptake. (usda.gov)
Roots are the principal organ for water and nutrient absorption and the location for synthesizing different hormones, organic acids, and amino acids. (techscience.com)

Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)

The cells that give rise to callus and somatic embryos usually undergo rapid division or are partially undifferentiated such as meristematic tissue. (wikipedia.org)
Compact calluses are typically green and sturdy, while friable calluses are white to creamy yellow in color, fall apart easily and can be used to generate cell suspension cultures and somatic embryos. (wikipedia.org)

Therefore, we investigated luciferase-mediated bioluminescence imaging (BLI) as a non-invasive technique to continuously monitor cellular behavior in ex vivo whole organ culture. (amsterdamumc.org)
Everything from how single cells in culture respond to their substrate to the way whole organ systems develop and respond to environmental stress. (lu.se)

The viruses attach to the host cells through hemagglutinin, which specifically combines with neuraminic acid receptors in the host cells. (medscape.com)

This provides important information on the development of the disease and the fact that severe cases of COVID-19 present with multi-organ failure and evidence of cardiovascular damage. (sciencebusiness.net)

The study detected with 90% power a difference in cell loss of approximately 10% or more. (nih.gov)
For this purpose, the use of cell culture chips allows users to study complex culture configurations by joining a culture well with a microfluidic channel via a porous membrane. (fluigent.com)
A previous study, which used a 3-dimensional (3-D) intestinal model derived from INT-407 cells reported NoV replication and extensive cytopathic effects (CPE). (cdc.gov)
To determine the potential cause of CPE observed in the previous study, we exposed 3-D cultures to lipopolysaccharide concentrations consistent with contaminated stool samples and observed morphologic features similar to CPE. (cdc.gov)
The lack of an efficient cell culture system in which to study NoV infections has hindered development of antiviral drugs to control or limit NoV outbreaks ( 5 ). (cdc.gov)
Senapati, S.K. and Rout, G.R. (2008) Study of Culture Conditions for Improved Micropropagation of Hybrid Rose. (scirp.org)
Liquid-phase electron microscopy, a new method for real-time nanoscopic imaging in liquid, makes it possible to study cells or biomolecules with a singular combination of spatial and temporal resolution. (rsc.org)
Our study provides new insights into how SARS-CoV-2 infects the cells of the body, including in blood vessels and kidneys," says Ali Mirazimi , adjunct professor at the Department of Laboratory Medicine at Karolinska Institutet and one of the study's corresponding authors. (sciencebusiness.net)
The current study was limited to cell cultures and engineered miniature organs. (sciencebusiness.net)
During the study of methionine- and choline-deficient diet-induced nonalcoholic fatty liver disease, we observed a specific decrease in the plasmacytoid dendritic cell (pDC) fraction from murine spleens. (bvsalud.org)

Together, our data demonstrate for the first time that perinatal exposure to THC triggers profound T cell dysfunction, thereby suggesting that the offspring of marijuana abusers who have been exposed to THC in utero may be at a higher risk of exhibiting immune dysfunction and contracting infectious diseases including HIV. (aspetjournals.org)
We've learned that appendiceal cancer has a distinctive genomic landscape and is surprisingly full of immune cells," said senior author Andrew Lowy, MD , chief of the Division of Surgical Oncology at Moores Cancer Center and a professor of surgery at UC San Diego School of Medicine. (ucsd.edu)

He has a professional background in research labs, genetics, and cell culturing. (selectbiosciences.com)

The interstitium, scientists found, is under our skin and between our organs. (nationalgeographic.com)
Lurking just under your skin might be a new organ only now identified for the first time, say a team of scientists. (nationalgeographic.com)
The organ has seemingly been hidden in plain sight, and scientists say they missed it because of the way tissue is studied. (nationalgeographic.com)
Scientific Bioprocessing (SBI) develops leading-edge instruments that make the work of cell scientists easier and more reproducible with optical sensing. (selectbiosciences.com)
SBI is changing the way cell scientists are able to optimize their bioprocessing applications and continues to introduce new and exciting technologies to enhance efficiency, reduce variability, and increase reproducibility with real-time monitoring. (selectbiosciences.com)
Scientists working in basic, translational, and clinical cancer metabolism research are invited to join the Academy in New York on April 17th to discuss the intersection between cell signaling and metabolism. (nyas.org)

This is the optimal device for Air Liquid Interface (ALI) culture, endothelium/epithelium barrier and crosstalk studies. (fluigent.com)

Lowy and colleagues developed an organotypic slice culture of living appendiceal cancer cells. (ucsd.edu)
Organotypic slices are three-dimensional cultures of an organ. (ucsd.edu)

The work of PulseNet provides insight into the lives of a bacterial cell through DNA pattern matches. (cdc.gov)
All 3 interpretations of lives of a cell the once independent lives of a single cell, the many lives of the earth, and the lives of a bacterial cell that travels throughout the earth strongly suggest a need for multidisciplinary and interdisciplinary collaborations, such as, One Health. (cdc.gov)

After four to five days of culture, the B cells started producing the IgA antibody in a process called "IgA class switch recombination. (sciencedaily.com)

As each of the cell progeny divides, the new pattern lives on in each new clone. (cdc.gov)

Unlike conventional meat, cultured muscle cells may be safer, without any adjacent digestive organs. (foodnavigator.com)

[6] In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. (wikipedia.org)

Within minutes after a small corneal epithelial injury, cells at the edge of the abrasion begin to migrate centripetally to cover the defect rapidly at a rate of 60-80 µm/h. (medscape.com)

If this is the same grains - such as soy, wheat and corn - associated with the production of conventional meat and deforestation -- then that puts the cell-based sector's environmental credentials in question. (foodnavigator.com)

In coeliac disease (CD) mucosa, the histological lesion is associated with marked infiltration of T helper cell type 1 (Th1) cells. (bmj.com)
There is now little doubt that in coeliac disease (CD) the histological lesion is associated with a predominant T helper cell type 1 (Th1) cell response. (bmj.com)
In a mouse experimental arthritis model, NKT17 cells are increased as the disease progresses, while NKT1 numbers negatively correlates with disease severity, with this protective effect of NKT1 linked to their IFN-γ expression. (nih.gov)
This will help us in finding organ/tissue specific potential therapeutic targets and also disease prevention by better understanding organ specific mechanisms behind initiation and progression of the disease. (lu.se)
Microphysiological systems mimic interconnections important in physiological or pathophysiological states of organ(s) and are often used for instance in HIGH THROUGHPUT SCREENING of drugs and factors important in disease modeling. (bvsalud.org)

It can be combined with M&S vitamins or Gamborg′s vitamins and supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture. (sigmaaldrich.com)

Genes can be inserted into callus cells using biolistic bombardment, also known as a gene gun, or Agrobacterium tumefaciens. (wikipedia.org)

All cell and organ cultures and materials of animal origin, including those from humans. (uwgb.edu)

The higher multiplication of single-node explant of 'rg01', 'rg02' and 'rg12' was cultured on half strength of MS medium supplemented with 2 mg L-1 BA and 5 g L-1 agar. (ncl.edu.tw)

BACKGROUND/AIM: Methionine addiction is a fundamental and general hallmark of cancer cells, which require exogenous methionine, despite large amounts of methionine synthesized endogenously. (bvsalud.org)

It can be easily transported from incubator to microscope for live cell imaging while maintaining cell perfusion under battery power. (fluigent.com)
The majority of neoplasms have mucinous histology, a characteristic that makes them difficult to assess under a microscope and to culture. (ucsd.edu)

cells are the most sensitive cell type of this epithelium. (cdc.gov)

Characterization of a primary cell cu. (ugent.be)
Characterization of a primary cell culture from lymphoid organ of Litopenaeus vannamei and use for studies on WSSV replication," AQUACULTURE , vol. 433, pp. 157-163, 2014. (ugent.be)

Microfluidic chips can now use geometries and structures to permit the use of physiological length scales, concentration gradients, and the mechanical forces generated by fluid flow to mimic the in vivo microenvironment experienced by cells. (fluigent.com)

The market's not just getting bigger but more diverse, and an increase in the variety of products available allows the 3D cell culture market to pursue a wider array of therapeutic targets. (corning.com)

Organ-on-chip cell culture platforms have proven potential in providing tremendous flexibility and robustness in drug screening and development by employing engineering techniques and materials. (fluigent.com)
The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
This review presents the three main approaches that have been taken to develop crops with Al tolerance: recurrent selection and breeding, development of Al tolerant somaclonal variants, and ectopic expression of transgenes to reduce Al uptake or limit damage to cells by Al. (usda.gov)

Anatomy82

Organisms19

Diseases11

Chemicals and Drugs44

Analytical, Diagnostic and Therapeutic Techniques and Equipment36

Phenomena and Processes28

Disciplines and Occupations2

Information Science2

Named Groups1

Health Care2

Vitro11

Tissues12

Plant Cell6

Internal organs1

Organoids7

Donor5

Proteins4

Differentiation3

Lungs5

Molecular1

Epithelial cells2

Vivo organ culture3

Corneas2

Researchers12

Adult stem1

Genetically3

Transplants1

Stem13

Drawbacks2

Mice2

Lymph2

Microbiological culture2

Grown2

20191

Mesophyll cells2

Organisms3

Endothelial1

Phenotype1

Microfluidics2

Shoots2

Biological2

Fetal1

Thymic1

Basal3

Human9

Vertebrate1

Viral2

Leaf2

Water and nutrient2

Biology1

Embryos2

Whole organ2

Receptors1

Multi-organ1

Study10

Immune2

Genetics1

Scientists6

Endothelium1

Organotypic2

Bacterial cell2

Antibody1

Clone1

Adjacent1

Embryonic1

Corneal epithelial1

Conventional1

Disease5

Generate1

Genes1

Humans1

Explant1

Exogenous1

Microscope2

Epithelium1

Characterization2

Mimic1

Therapeutic targets1

Development3