Cells in human skin human skin cells. Medical search. Frequent questions

Protein expression Collagen peptide Human skin fibroblasts cells UVA. (ajol.info)
In this study, we reported the cloning and over expression of a gene coding for human collagen peptide (CP6) in Escherichia coli and investigated the protective effects of CP6 on UVA-irradiated human skin fibroblasts cells. (ajol.info)
Also, we performed assays with 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT), Annexin V-fluorescein isothiocyanate/propidine iodide (Annexin V-FITC/PI) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) methods to investigate the cytoprotective effects of CP6 on the proliferation of UVA-damaged human skin fibroblasts cells. (ajol.info)
Radiation dosages (5 J/cm 2 ) significantly decreased the proliferation activities of human skin fibroblasts cells (HSF). (ajol.info)
UVA could damage the human skin fibroblasts cell in vitro . (ajol.info)
SALPs inhibited LP-induced phosphorylation of NF-κB p65 and p38 MAPK and reduced cytokine release and gene expression in primary human keratinocytes and dermal fibroblasts. (nature.com)
Therefore, we examined here intracellular AA levels and stability throughout its exposure to human skin fibroblasts in vitro. (nih.gov)
Thus, these results indicate that human skin fibroblasts exposed to AA over time had rising levels of type 1/type 4 collagens and SVCT2 mRNA expression and type 1 procollagen synthesis. (nih.gov)
Keratinocytes and fibroblasts of epidermal and dermal layers of the skin play prominent roles in this process. (indexindex.com)
In cell proliferation assays, asiaticoside induced an increase in the number of normal human dermal fibroblasts. (indexindex.com)
FBN2 silencing recapitulates hypoxic conditions and induces elastic fiber impairment in human dermal fibroblasts. (mcgill.ca)
My Ph.D. studies have focused on developing ways to generate human astrocytes from pluripotent stem cells and fibroblasts for modeling diseases affecting the human brain. (lu.se)

The models tested included melanoma cells, neonatal melanocytes and keratinocytes, and immortalized HaCaT keratinocytes. (biologists.com)
Furthermore, SALPs markedly promoted cell migration via EGFR transactivation and ERK1/2 phosphorylation and accelerated artificial wound closure in keratinocytes. (nature.com)
1999. Response of normal human keratinocytes to sulfur mustard (HD): Cytokine release using a non-enzymatic detachment procedure. (cdc.gov)
A postdoctoral research associate in my lab, Masakazu Kurita , who has a background in plastic surgery, knew that a critical step in wound healing was the migration of stem-cell-like cells called basal keratinocytes-from nearby, undamaged skin-into wounds. (singularityhub.com)
Basal keratinocytes are precursors to many different types of skin cells. (singularityhub.com)
We wanted to convert these mesenchymal cells into basal keratinocytes, without ever taking them out of the body. (singularityhub.com)
We conducted further experiments on each potential reprogramming factor and narrowed the list down to four factors that would transform mesenchymal cells into basal keratinocytes in vitro in petri dishes. (singularityhub.com)
These keratinocytes then formed all the cells present in healthy new skin. (singularityhub.com)
Green is keratinocytes, red is mesenchymal cells, blue is the cell nucleus and magenta marks the innermost layer of skin epithelium. (singularityhub.com)
These four factors reprogrammed the mesenchymal cells in the wound into keratinocytes which then grew into the many cells types that make up healthy skin, closing and healing the sore. (singularityhub.com)
Human epithelial cells induce human melanocyte growth in vitro but only skin keratinocytes regulate its proper differentiation in the absence of dermis. (unimore.it)
Human keratinocytes isolated from a skin biopsy and cultured in vitro reconstitute a stratified squamous epithelium suitable for grafting on burned patients. (unimore.it)
b) melanocyte growth is not autonomous but requires close keratinocyte contact and is regulated to maintain a physiological melanocytes/keratinocytes ratiol and (c) pure skin keratinocytes, but not oral epithelial cells, have all the information required for the proper physiological location and differentiation of melanocytes in the epidermis. (unimore.it)
Psoriatic skin is marked by increased proliferation of keratinocytes the major cell of the outermost layer of skin resulting in a thickening of the epidermis. (bio2009.org)
Massive ex vivo expansion of epidermal cells (called keratinocytes) is needed for the production of grafts. (cea.fr)
It is performed using a skin sample from the patient that contains adult keratinocytes and a minority population of keratinocyte stem cells. (cea.fr)
In the future, keratinocytes produced from pluripotent stem cells could be an alternative to adult stem cells in certain reconstructed tissue bio-engineering applications. (cea.fr)
One of the difficulties encountered in this area is that fact that the keratinocytes obtained do not have all the functions of adult stem cells. (cea.fr)
The study has shown that manipulation of KLF4 expression is also suitable for these cells, as reducing its expression in keratinocytes derived from embryonic stem cells (ESC) improves their proliferation capacity and their ability to reconstruct skin. (cea.fr)
Whereas the expression of syndecan-4 and glypican in intact skin was mostly in the pericellular regions of keratinocytes, the epidermal cells from the wound edge contained mostly intracellular PGs. (lu.se)

The research team had set up a system to grow human skin in the lab, starting with induced pluripotent stem cells, naïve adult human cells with the potential to give rise to many other kinds of tissues. (alleninstitute.org)
Investigators took skin cells biopsied from volunteers with an inherited form of glaucoma and from volunteers without the disease and genetically reprogrammed them to become pluripotent stem cells. (siasat.com)
The researchers started out with human pluripotent stem cells, which can differentiate into various cell types. (israel21c.org)
The researchers reprogrammed the pluripotent stem cells to an earlier (naïve) stage corresponding to day 7 of a natural human embryo, around the time it implants itself in the womb. (israel21c.org)
By using a new approach, we developed a rapid and efficient method to generate functional and mature astrocytes from human pluripotent stem cells through overexpression of certain transcription factors that control the development of astrocytes. (lu.se)

The researchers showed they could convert human embryonic stem cells to neurons by infecting them with a virus that expressed the same proteins used in the study. (stanforddaily.com)
This treatment, nicknamed "BAM" after an acronym of the three proteins, converted the embryonic stem cells into functional neurons within six days. (stanforddaily.com)
In a feat of bioengineering, scientists take adult skin cells and then essentially reprogram them to turn them into embryonic stem cells that can be grown into all manner of cells. (naturalnews.com)
A paper appearing in the online version of Cell this week describes the successful reprogramming of human skin cells into embryonic stem cells. (bigthink.com)
Scientists have managed to create synthetic human embryo models without using egg, sperm or womb, in a feat that could impact research on fertility, tissue growth and drug testing, as well as improve science's understanding of the first weeks of embryonic development. (israel21c.org)
These embryo-like structures went on to develop for eight days outside the womb, reaching a stage equivalent to day 14 in natural human embryonic development. (israel21c.org)
Scientists at Oregon Health & Science University and the Oregon National Primate Research Center (ONPRC) have successfully reprogrammed human skin cells to become embryonic stem cells capable of transforming into any other cell type in the body. (ohsu.edu)
The research breakthrough, led by Shoukhrat Mitalipov, Ph.D. , a senior scientist at ONPRC, follows previous success in transforming monkey skin cells into embryonic stem cells in 2007. (ohsu.edu)
A thorough examination of the stem cells derived through this technique demonstrated their ability to convert just like normal embryonic stem cells, into several different cell types, including nerve cells, liver cells and heart cells. (ohsu.edu)
In 2000, the National Institutes of Health issued guidelines for the use of embryonic stem cells in research, specifying that scientists receiving federal funds could use only extra embryos that would otherwise be discarded. (cnn.com)
Above, a human stem cell colony, which is no more than 1 millimeter wide and comprises thousands of individual stem cells, grows on mouse embryonic fibroblast in a research laboratory in September 2001. (cnn.com)
In March 2009, President Barack Obama signed an executive order that removed restrictions on embryonic stem cell research. (cnn.com)
His action overturned an order approved by President George W. Bush in August 2001 that barred the National Institutes of Health from funding research on embryonic stem cells beyond using 60 cell lines that existed at that time. (cnn.com)
In November 2010, William Caldwell, CEO of Advanced Cell Technology, said the FDA had granted approval for his company to start a clinical trial using cells grown from human embryonic stem cells. (cnn.com)
Above, dozens of packages containing frozen embryonic stem cells remain in liquid nitrogen in a laboratory at the University of Sao Paulo's human genome research center in Sao Paulo, Brazil, in March 2008. (cnn.com)

Compared to studying individual cells in a petri dish, organoids allow researchers to study cells in more lifelike environs, as the clumps of tissue will form different types of structures akin to real organs in the body. (alleninstitute.org)
Through the course of that project, which used mouse stem cells rather than human cells, he and his colleagues managed to generate skin tissue as a by-product. (alleninstitute.org)
Ironically, the Allen Cell Collection is built from stem cells first generated at the Gladstone Institutes from an adult donor's skin cells - although that doesn't mean the stem cells are more similar to skin than they are to other types of tissue. (alleninstitute.org)
This partial amino acid sequence corresponded to the sequence of human heart chymase, a proteinase isolated from heart tissue with immunologic and hydrolytic properties similar to skin chymase. (duke.edu)
Such treatments include allogeneic and xenogeneic transplants and skin substitutes such as tissue-engineered skin, cultured cells, and stem cells. (biomedcentral.com)
The aim of this paper is to discuss the general overview of the quality assurance and quality control implemented in the manufacturing of cell and tissue product, with emphasis on our experience in the manufacturing of MyDerm ® , an autologous bilayered human skin substitute. (biomedcentral.com)
Manufacturing MyDerm ® requires multiple high-risk open manipulation steps, such as tissue processing, cell culture expansion, and skin construct formation. (biomedcentral.com)
In recent years, the development in cell therapy and stem cell research has led scientist to engage in the production of cells and tissue product that is of clinical grade. (biomedcentral.com)
Circulating memory T cells can be divided into tissue-specific subsets, which traffic through distinct tissue compartments during physiologic immune surveillance, based on their expression of adhesion molecules and chemokine receptors. (biomedcentral.com)
Consequently, circulating T cells can be divided into tissue specific subsets, each of which have the ability to traffic through certain tissue compartments, but which are excluded from others. (biomedcentral.com)
When a tissue experiences inflammation, its cells remember. (scitechdaily.com)
This is particularly relevant for bacterial skin and soft-tissue infections (SSTIs) being most commonly caused by multidrug-resistant bacteria with Staphylococcus aureus as predominant causative Gram-positive bacterium and Pseudomonas aeruginosa as most frequent Gram-negative bacterium and having fatal consequences if treated unproperly 2 . (nature.com)
The research themes rank around molecular mechanisms matrix proteins and fibers exert in disorders affecting blood vessels, bone, skin and adipose tissue. (mcgill.ca)
Rodent skin heals differently from human skin, so there was no visible scar tissue, though it should have been there.Further work is necessary to ensure the safety of this approach, especially over a much longer term, but as an initial test of the concept, the results are very promising. (singularityhub.com)
We are optimistic that our approach represents an initial proof of principle for in vivo regeneration of an entire three-dimensional tissue, like the skin, not just individual cell types. (singularityhub.com)
A CEA-Jacob team has just published a paper in which it demonstrates the central role of the transcription factor KLF4 in regulating the proliferation of epidermal stem cells and their ability to regenerate this tissue. (cea.fr)
Human skin completely renews itself every month thanks to the presence of stem cells in the deepest layer, which generate all the upper layers of this tissue. (cea.fr)
The discoveries of a French research team from the CEA, INSERM and the University of Paris, produced in collaboration with I-Stem, the AFM-Téléthon laboratory, and the University of Évry, opens perspectives for regenerative cutaneous medicine, in particular for the bio-engineering of skin grafts for tissue reconstruction. (cea.fr)
The Wake Forest Institute for Regenerative Medicine (WFIRM) researchers, who were the first to identify that stem cells in human urine have potential for tissue regenerative effects, continue their investigation into the power of these cells. (phys.org)
Their findings provide a novel perspective to evaluate the capacity of telomerase-positive human urine -derived stem cells to become a wide variety of other cell types, and to be used as an optimal cell source for stem cell therapy or cell-based tissue regeneration. (phys.org)
WFIRM's Yuanyuan Zhang, Ph.D, lead author of the paper published by Frontiers in Cell and Developmental Biology , said that human urine-derived stem cells can be easily isolated from urine samples which offers clear advantages over stem cells from other sources, like bone marrow or fat tissue which often require a surgical procedure for collection. (phys.org)
As a safe cell source, telomerase-positive human urine-derived stem cells have a robust regenerative potential, which might induce better tissue repair," said Zhang. (phys.org)
In February 2012, early research published by scientists at Cedars-Sinai Medical Center and Johns Hopkins University showed that a patient's own stem cells can be used to regenerate heart tissue and help undo damage caused by a heart attack. (cnn.com)
a germicide that is used on skin or living tissue for the purpose of inhibiting or destroying microorganisms. (cdc.gov)
In May 2010, the Sixty-third World Health Assembly adopted resolution WHA63.22,1 in which it endorsed the updated WHO Guiding Principles on Human Cell, Tissue and Organ Transplantation and provided strategic directions to support progress in human organ, tissue and cell donation with the aim of maximizing the benefits of transplantation, meeting the needs of recipients, protecting donors and ensuring the dignity of all involved. (who.int)
The benefits of human tissue transplantation can be seen in both children and adults, including in survival rates following severe burn trauma, recovery of movement, closure of chronic wounds, rehabilitation of heart function and restoration of sight. (who.int)
It is very difficult to develop therapeutics for central nervous system disorders owing to several challenges in studying the human brain due to its exceptionally high complexity that is not present in other animal species, and inaccessibility to tissue samples from patients and healthy individuals. (lu.se)

Scholars@Duke publication: Determination of the primary structures of human skin chymase and cathepsin G from cutaneous mast cells of urticaria pigmentosa lesions. (duke.edu)
The deduced amino acid sequence between the primers was identical to that reported for neutrophil cathepsin G, indicating that the protein of cutaneous mast cells previously shown to be immunologically cross-reactive with neutrophil cathepsin G has a comparable amino acid sequence. (duke.edu)
For instance, studies in mice have demonstrated that CD4+ T cells activated in cutaneous lymph nodes upregulate trafficking determinants specific for the skin, such as P-selectin ligand, while T cells responding to antigen in intestinal lymph nodes express high levels the gut-associated adhesion molecule integrin α4β7 and acquire responsiveness to the intestinal CC-chemokine ligand CCL25 [ 2 ]. (biomedcentral.com)
These defects include impaired cutaneous responses to recall antigens, decreased ratio of circulating T-helper cells to suppressor cells, impaired lymphocyte proliferative responses to mitogen, impaired production of interferon in lymphocytes, and reduced natural killer cell activity. (medscape.com)
People with severe burns, bedsores, or chronic diseases such as diabetes are at risk for developing wounds known as cutaneous ulcers, which can extend through multiple layers of the skin. (singularityhub.com)
This work has been extended to other types of cells of interest for cutaneous cell therapy. (cea.fr)
The purpose of this article is to integrate renal and cutaneous aspects of disease as well as highlight some important, although frequently underappreciated, clinical or laboratory findings that ally renal and skin diseases. (medscape.com)
Natural History of Incident and Persistent Cutaneous Human Papillomavirus and Human Polyomavirus Infections. (who.int)

When in vitro fertilization was first developed, a lot of people were disturbed by the idea of creating a baby outside of the human baby - and many still are. (naturalnews.com)
Scientists are expected to have the ability to create babies from human skin cells within the next two decades in a process known as in vitro gametogenesis, or IVG. (naturalnews.com)
To explore the influence of human amniotic mesenchymal stem cells (hAMSCs) on the in vivo and in vitro regulation of macrophage phenotypes and inflammatory factors associated with wound healing of full-thickness skin wounds in mice . (bvsalud.org)
Peptide fractions extracted from Flathead by-products were evaluated for in vitro free radical scavenging and cancer cell cytotoxic activities. (springer.com)
Using in vitro systems, we observed the effects of asiaticoside on normal human skin cell behaviors related to healing. (indexindex.com)
Methodologically, the lab employs a wide spectrum of techniques ranging from in vitro biochemical and biophysical assays, to cell biology methods with primary cells and transformed cell lines, to light and electron microscopic techniques, to in vivo studies with genetically modified mouse models. (mcgill.ca)
To investigate the mechanisms of anaphylactoid reactions to radiocontrast media, in vitro mediator release induced by three iodinated contrast agents was examined using peripheral blood basophils and mast cells purified from human lung parenchyma, heart, and skin tissues. (johnshopkins.edu)
In vitro cleavage of diisocyanate -glutathione conjugates by human gamma-glutamyl transpeptidase-1. (cdc.gov)

We determine a comprehensive map of lineage-specific lncRNAs in human dermal lymphatic and blood vascular endothelial cells (LECs and BECs), combining RNA-Seq and CAGE-Seq. (nature.com)
Here, we evaluated dermal toxicity of ionic liquids using human keratinocyte and fibroblast cell line, 3D reconstructed human epidermis, and full-thickness model to investigate underlying mechanisms. (elsevierpure.com)

This study establishes the primary structure of human skin chymase and provides further evidence for the presence of a cathepsin G-like proteinase within human mast cells. (duke.edu)
UP is a disease characterized by skin lesions containing high numbers of mast cells. (duke.edu)
These studies provide further evidence that human skin mast cells contain two different chymotrypsin-like proteinases. (duke.edu)
Three iodinated contrast agents, sodium and meglumine salts of ioxaglic acid, sodium and meglumine salts of ioxithalamic acid, and ioversol, were incubated with basophils purified from peripheral blood and human mast cells isolated and purified from different anatomical sites. (johnshopkins.edu)
All three induced the release of preformed mediators (histamine and tryptase) from human lung, but not from skin mast cells. (johnshopkins.edu)
They also induced histamine and tryptase release from human heart mast cells. (johnshopkins.edu)
Cross-linking of IgE by anti-IgE induced the release of leukotriene C 4 or prostaglandin D 2 from human basophils or mast cells. (johnshopkins.edu)
Mannitol, an osmotic stimulus, induced the release of histamine from human basophils, but to a lesser extent from mast cells. (johnshopkins.edu)
These results show that different contrast media can differ in their ability to release mediators from enriched preparations of human basophils and mast cells. (johnshopkins.edu)
The three contrast agents examined act on basophils and mast cells as incomplete secretagogues, causing the release of preformed mediators, but not the de novo synthesis of chemical mediators. (johnshopkins.edu)

DENV-infected LCs efficiently transmitted DENV to target cells such as dendritic cells (DCs). (nih.gov)
This is happening in natural killer cells, T cells, dendritic cells from human skin, and epidermal stem cells in mice," says Samantha B. Larsen, a former graduate student in the laboratory of Elaine Fuchs at The Rockefeller University. (scitechdaily.com)
In LPS-stimulated human monocyte-derived dendritic cells and Langerhans-like cells, the peptides blocked IL-6 secretion, downregulated expression of maturation markers and inhibited dendritic cell migration. (nature.com)

In the present study, the venoms from five sea anemones (Heteractis crispa, Heteractis magnifica, Heteractis malu, Cryptodendrum adhaesivum and Entacmaea quadricolor) were obtained by the milking technique, and the potential of these venoms to kill cancer cells was tested on three cell lines (A549 lung cancer, T47D breast cancer and A431 skin cancer). (scielo.br)
The results indicate that the sea anemone venom is cytotoxic to human cancer cells. (scielo.br)
sea anemone venoms, cytolysin, cancer cell line. (scielo.br)
Genetic studies in mice have shown that mutation of the CK2 phospho-acceptor site in p53 increases UV-induced skin cancer formation,(11) highlighting an unexpected role for p53 phosphorylation in mediating p53-dependent tumor suppression. (dundee.ac.uk)
3 kDa inhibited the growth of HT-29 colon cancer cells up to 91.04 %, although the activity was found to be non-selective. (springer.com)
In: Mukhtar H (ed) Skin cancer: mechanisms and human relevance. (springer.com)
A gene recently linked to liver, skin, and pancreatic cancer also lies behind an often deadly form of breast cancer. (sciencenews.org)
The protein made by a gene called RhoC helps both normal and cancer cells move, says Kenneth van Golen of the University of Michigan Health System in Ann Arbor. (sciencenews.org)
The DeGroote facility is shared with the Centre for Function Genomics, Centre for Gene Therapeutics, Institute for Cancer and Stem Cell Biology Research, Robert E. Fitzhenry Vector Laboratory, Centre for Asthma and Allergy Research (Allergen) and North American Headquarters for West Nile studies, as well as the Bachelor of Health Sciences undergraduate program. (wikipedia.org)
In addition to wound healing, our approach could be useful for repairing skin damage, countering the effects of aging and helping us to better understand skin cancer. (singularityhub.com)
Skin cancer is the most common form of cancer in the United States. (medlineplus.gov)
The two most common types are basal cell cancer and squamous cell cancer. (medlineplus.gov)
Another type of skin cancer, melanoma , is more dangerous but less common. (medlineplus.gov)
If not treated, some types of skin cancer cells can spread to other tissues and organs. (medlineplus.gov)
PDT uses a drug and a type of laser light to kill cancer cells. (medlineplus.gov)
What Can I Do to Reduce My Risk of Skin Cancer? (medlineplus.gov)
What's New in Basal and Squamous Cell Skin Cancer Research? (medlineplus.gov)
Better understanding of alterations in this cell subpopulation throughout the human lifespan, and how they translate into, aging, kidney damage, or cancer, among others will be beneficial overall. (phys.org)
Screening finds abnormal cells on the cervix so they can be treated before they turn into cancer. (cdc.gov)
Transcriptomic profiling of T-cell populations in non-muscle invasive and muscle invasive bladder cancer. (lu.se)

GENERAL INFORMATION Human A375 Skin Melanoma Cells were isolated from a female patient with malignant skin melanoma. (ubpone.com)
Here we investigated the effect Cisplatin (CDDP), one of the most potent chemotherapeutic agent used to treat tumors, in association with fasting in wild type and mutated BRAF(V600E) melanoma cell lines. (unisalento.it)
In addition, we show that autophagy is not involved in the enhanced sensitivity of melanoma cells to combined CDDP/EBSS-induced apoptosis. (unisalento.it)

For much of its history psoriasis was believed to be solely a disorder of the skin characterised by aberrant keratinocyte activity. (bio2009.org)

Stem cell therapies have long been seen as a holy grail treatment for many common diseases including multiple sclerosis and Parkinson's disease, yet many have questioned the ethics of using developing human embryos for this purpose. (bigthink.com)
associated with skin-homing) as well as the expression of integrin α4β7 and CCR9 (associated with gut-homing) was analyzed on CD4+ memory T cells in CSF from individuals with non-inflammatory neurological diseases using flow cytometry. (biomedcentral.com)
Diseases or conditions that might be treated through stem cell therapy include Parkinson's disease, multiple sclerosis, cardiac disease and spinal cord injuries. (ohsu.edu)
This is a remarkable accomplishment by the Mitalipov lab that will fuel the development of stem cell therapies to combat several diseases and conditions for which there are currently no treatments or cures," said Dr. Dan Dorsa, Ph.D. , OHSU Vice President for Research. (ohsu.edu)
This achievement has significant implications for understanding pregnancy development, studying pregnancy-related diseases, and advancing cell therapies. (austfhu.org.au)
2. University of Évry, University of Paris-Saclay, INSERM U861, Institute for Stem Cell Therapy and Exploration of Monogenic Diseases (I-Stem), 91100 Corbeil Essonne, France. (cea.fr)
3. Centre for the Study of Stem Cells (CECS), Institute for Stem Cell Therapy and Exploration of Monogenic Diseases (I Stem), 91100 Corbeil Essonne, France. (cea.fr)
Experts in the field of regenerative medicine believe one of the first areas of success when using stem cell-derived therapies will be the treatment of macular degeneration, which causes progressive loss of sight, and other retinal diseases. (cnn.com)
The transplantation of human tissues, organs or cells is an established form of treatment that has been acknowledged as the best and very often only life-saving therapy for several serious and life-threatening congenital, inherited and acquired diseases and injuries. (who.int)
Haematopoietic stem cell transplants have been performed in more than 1 500 000 patients (both autologous and allogeneic) to date.1 Although haematological cancers remain the main indication, haematopoietic stem cell transplants are increasingly considered in the treatment of non-malignant disorders and genetic diseases such as haemoglobinopathies (sickle cell anaemia, thalassaemia) that can benefit greatly from this type of transplant. (who.int)
Instead, by developing models in the dish with high biological relevance and predictive value for the human brain, we can accelerate our knowledge about what occurs during diseases affecting humans. (lu.se)
We focused on one of the main cell types found in our brains, the astrocyte, which over the last decade has emerged as an important contributor to a wide range of neurological diseases. (lu.se)

Influence of human amniotic mesenchymal stem cells on macrophage phenotypes and inflammatory factors in full-thickness skin wounds of mice]. (bvsalud.org)

By not using actual embryos, scientists at two Oregon-based institutions sidestepped one of the largest issues surrounding stem cell technology. (bigthink.com)
In the laboratory of Prof. Jacob Hanna at the Weizmann Institute of Science , researchers created complete models of human embryos from stem cells cultured in a lab grew them up to day 14. (israel21c.org)

These are able to differentiate into any cell type in the body. (siasat.com)
During development, the blood vascular system arises from endothelial cell progenitors that differentiate from mesodermal cells, mostly through the expression of the transcription factor (TF) ETV2. (nature.com)
Activation of the VEGFA/VEGFR2 signaling and expression of blood vascular endothelial cell (BEC) markers, such as NRP1 and EphrinB2, further differentiate these precursor cells into BECs, which then form the hierarchical network of blood vessels 4 . (nature.com)
We are interested how extracellular matrix proteins instruct cells to differentiate, migrate, and proliferate, and how cells sense normal and pathologically altered extracellular matrix. (mcgill.ca)

Scientists from the Oregon Health & Science University and the Oregon National Primate Research Center figured out that the fragility of human egg cells - into which a nucleus from another human cell, in this case skin, is transferred and begins to produce stem cells - required a different approach. (bigthink.com)
Based on a better understanding of the basement membrane zone (BMZ) and the genes responsible for its components, newer treatments (eg, gene or protein therapy) may provide solutions to the skin fragility found in patients with epidermolysis bullosa. (medscape.com)

The researchers found that BAM treatment to skin cells from fetuses and newborns didn't have the same effect as it did on the stem cells. (stanforddaily.com)
The researchers say that cells from women could be used to produce sperm, but that sperm would only be able to produce female babies because they lack a Y chromosome. (naturalnews.com)
Researchers have succeeded in growing the most lifelike human skin in the lab to date. (alleninstitute.org)
They turned to cell lines from the Allen Cell Collection , a suite of human stem cells gene-edited by researchers at the Allen Institute for Cell Science, a division of the Allen Institute, to tag certain parts of the cell with fluorescent labels. (alleninstitute.org)
These glowing structures allowed the researchers to better track the formation of different skin layers in the organoids - they could see the epidermis developing in real-time as the fluorescent signal got brighter under the microscope. (alleninstitute.org)
New York: Using stem cells derived from human skin cells, US researchers have revealed that these can be turned into retinal ganglion cells (RGCs) - the neurons that conduct visual information from the eye to the brain - to prevent or cure glaucoma. (siasat.com)
Researchers then directed the stem cells to become RGCs, at which point the cells began adopting features specific to RGCs - features that were different in the cells of individuals with glaucoma than in the cells that came from healthy individuals. (siasat.com)
Prof. Jacob Hanna (center) and his team of researchers working on the development of the stem-cell embryo models. (israel21c.org)
Through moving findings between monkey cells and human cells, the researchers were able to develop a successful method. (ohsu.edu)
In such cases, researchers may isolate skin stem cells from a patient , grow them in the laboratory and transplant them back into the patient. (singularityhub.com)
In January 2014, researchers announced they had developed a new method of making stem cells: by placing skin cells in an acidic environment. (cnn.com)

The differentiation of naïve T cells into an activated memory phenotype is characterized by an extensive change in the expression of trafficking determinants, resulting in the acquisition of homing receptors that enable the cells to migrate from the circulation into peripheral tissues. (biomedcentral.com)
Using conventional methods, you are only able to grow tissues which are a few millimetres thick, which is fine for growing artificial skin, but if you wanted to grow cartilage, for instance, it would be impossible. (bbc.co.uk)
Connective tissues weave your different body parts - skin, bones, and organs - together and give your body shape. (webmd.com)
While the development of xenografts (products of animal origin) and bioengineered alternatives is advancing, to date they cannot fully mimic or replace all human-sourced tissues on an equivalent basis. (who.int)
Thus, the availability of and access to human tissues for transplantation remains essential. (who.int)
In June 2018, the Secretariat established the WHO Task Force on Donation and Transplantation of Human Organs and Tissues as an advisory group composed of experts from all WHO regions. (who.int)

With the addition of four proteins, adult human skin cells can be transformed into neurons over a month-long period. (stanforddaily.com)
Pinning proteins to its genetic material at the height of inflammation, the cells bookmark where they left off in their last tussle. (scitechdaily.com)
To do so, we compared the levels of different proteins inside the two cell types to figure out what distinguished them and find out what we would need to change in order to reprogram one cell type into the other. (singularityhub.com)

The University of Manchester team say the inkjets will be able to "print out" tailor-made human cells to fit a patient's exact dimensions. (bbc.co.uk)
Being able to use a patient's own stem cells for therapy is considered advantageous because they do not induce immune responses or rejection," said WFIRM Director Anthony Atala, MD, who is a co-author of the paper. (phys.org)
A follow-up investigation of rat breeders who supplied the initial patient's rats revealed six additional human cases of Seoul virus infections occurring at two Illinois rat-breeding facilities. (cdc.gov)

He expressed hope that one day the cells could be used for human therapies. (stanforddaily.com)
It is believed that stem cell therapies hold the promise of replacing cells damaged through injury or illness. (ohsu.edu)
The presence of a large number of immune cells in psoriatic skin suggested that they play a part in the disease and the discovery that therapies targeting the immune system were effective for the treatment of psoriasis further highlighted its importance. (bio2009.org)

it triggered the skin cells' transformation into functional neurons within about four to five weeks. (stanforddaily.com)
The cells expressed electrical activity characteristic of neurons and even integrated and interacted with mouse neurons on a laboratory dish. (stanforddaily.com)
While they found that approximately 20 percent of mouse skin cells transform directly into functional neurons, under current culture conditions only about two to four percent of human skin cells do the same. (stanforddaily.com)
Although, there is still an incomplete understanding of how the teamwork between astrocytes, neurons, and other cells in the brain is carried out and what consequences occur when astrocytes stop working properly. (lu.se)

1998. Protection of human upper respiratory tract cell lines against sulphur mustard toxicity by hexamethylenetetramine (HMT). (cdc.gov)
However, little is known about toxicity of ionic liquids on the skin, a major exposure portal to toxic substances. (elsevierpure.com)
In 3D reconstructed human epidermis and full-thickness model, a single application of [TFSI] induced toxicity although it was minimal and largely limited to epidermal layer. (elsevierpure.com)
The toxicity information for some of the health effects listed above may be categorical in nature (presence/absence of genotoxicity or skin irritation, for example) while other outcomes are expressed through quantitative information and/or potency data. (cdc.gov)
At that time, data were insufficient to assess the efficacy of ZDV as a prophylactic agent in humans or the toxicity of this drug in persons not infected with HIV. (cdc.gov)

Recent genetic studies have identified a range of potential molecules relating to NK cell biology that are known to be important in psoriasis. (bio2009.org)
Yingai Shi et al, Differentiation Capacity of Human Urine-Derived Stem Cells to Retain Telomerase Activity, Frontiers in Cell and Developmental Biology (2022). (phys.org)
Development: For advances in developmental biology and stem cells. (lu.se)

Thereafter, a distinct subpopulation of endothelial cells lining the cardinal vein starts differentiating by expressing the TF PROX1, the master regulator of lymphatic endothelial cell (LEC) identity, via the TFs SOX18 and COUPTFII. (nature.com)

A group of scientists outlined serious reservations about the consequences of such technology, writing in the Science Translational Medicine journal: "I.V.G. may raise the specter of 'embryo farming' on a scale currently unimagined, which might exacerbate concerns about the devaluation of human life. (naturalnews.com)
Scientists have long suspected that even cells that are not traditionally involved in the immune response have the rudimentary ability to remember prior insults and learn from experience. (scitechdaily.com)
Scientists are developing an inkjet printer that can create "made to measure" skin and bones to treat people with severe burns or disfigurements. (bbc.co.uk)
Scientists view stem cells as a possible gateway to curing many medical conditions, from Parkinson's disease to diabetes. (cnn.com)

and even, one day, as the basis for better skin grafts for burn victims or other surgical uses. (alleninstitute.org)
KLF4 is therefore a new molecular target for preserving the functionality of stem cells and making progress in the bio-engineering of skin grafts. (cea.fr)

So far, it has only been used in mice, but it is believed to be just a matter of time before it can be used for human reproduction. (naturalnews.com)
2) Full-thickness skin wound model was reproduced in the back of 56 C57BL/6 mice , which were divided into hAMSCs group and phosphate buffer solution (PBS) group using the random number table, with 28 mice in each group. (bvsalud.org)
Mice in hAMSCs group were subcutaneously injected with 100 µL of cell suspension containing 1×10(7) hAMSCs per mL in PBS suspension along the wound edge. (bvsalud.org)
So the Fuchs lab once again exposed mice skin to irritants, and watched as stem cells in the skin changed. (scitechdaily.com)
We then tested the power of these four factors to treat skin ulcers on mice. (singularityhub.com)
When we examined the mice three months and six months later, we saw that the newly generated cells functioned like healthy skin. (singularityhub.com)

Dual-immunofluorescence also revealed that Ser392 and Ser15 phosphorylation of p53 also occur in the same basal cells, although often within distinct regions of the nucleus. (dundee.ac.uk)
It involves transplanting the nucleus of one cell, containing an individual's DNA, into an egg cell that has had its genetic material removed. (ohsu.edu)

While, the exposure to 2-DG further enhanced the apoptotic rate observed in SK Mel 28 cells upon treatment with both CDDP and EBSS. (unisalento.it)
The presence of bioactive peptides during H 2 O 2 exposure increased viability of T4056 normal cells. (springer.com)
Research has also shown that following a significant exposure of the skin to the ultraviolet light, the amounts of these antioxidants present in the skin are significantly depleted. (mattek.com)

Global burden of HPV-attributable squamous cell carcinoma of the anus in 2020, according to sex and HIV status: A worldwide analysis. (who.int)

It had layers of dermis and epidermis, just like real skin, and tiny mouse hair follicles. (alleninstitute.org)
The outer layer of skin, the epidermis, is chock full of desmosomes. (alleninstitute.org)
Inflamed mouse stem cells located in the basal layer (red) of the epidermis and FOS (green), a near-universal stress response factor essential to inflammatory memory. (scitechdaily.com)

Metaphase is a stage in the cell's natural division process (meiosis) when genetic material aligns in the middle of the cell before the cell divides. (ohsu.edu)
1991. Genetic activity of the human carcinogen sulphur mustard towards salmonella and the mouse bone marrow. (cdc.gov)
This paper will discuss the evidence 7-Methyluric Acid both cellular and genetic for NK cell involvement in psoriasis. (bio2009.org)
There is marked angiogenesis and infiltration of immune cells into the skin [1 2 The cause of psoriasis is still unknown although it is clear that there is a strong genetic component to the disease. (bio2009.org)

The A549 cell line was the most sensitive of the cell lines tested with a significant reduction in viability observed at 40 µg/mL. (scielo.br)
Viability of cultured primary human skin cells treated with HDI monomer and HDI isocyanurate. (cdc.gov)

Their latest study, published in the prestigious journal Nature Communications, reveals a remarkable breakthrough in converting skin samples into functional human placenta cells . (austfhu.org.au)
1. A single functional stem cell is able to regenerate the skin throughout a person's life. (cea.fr)

Although preventing blood exposures is the primary means of preventing occupationally acquired human immunodeficiency virus (HIV) infection, appropriate postexposure management is an important element of workplace safety. (cdc.gov)
Infectious causes of glomerulonephritis included streptococcal infections, human immunodeficiency virus (HIV) infection, and hepatitis viral infections, both hepatitis C (HCV) and hepatitis B (HBV). (medscape.com)

It is highly recommended to perform cell manufacturing for therapeutic use in a well-designed GMP-compliant cleanroom facility. (biomedcentral.com)
One important distinction is that while the method might be considered a technique for cloning stem cells, commonly called therapeutic cloning, the same method would not likely be successful in producing human clones otherwise known as reproductive cloning. (ohsu.edu)
Here, we investigated the potential of Pep19-2.5 and the structurally related compound Pep19-4LF for their therapeutic application in bacterial skin infections. (nature.com)
Conclusively, our data suggest a novel therapeutic target for the treatment of patients with acute and chronic skin infections. (nature.com)
and as a bioactive component of an artificial skin, may have therapeutic value. (indexindex.com)

The research team has already shown that in a mouse model of wound healing, these little skin blobs flatten out and grow into a skin sheet, complete with tufts of black hair. (alleninstitute.org)
We're hoping we can use skin organoids kind of like seeds to generate new skin in a wound. (alleninstitute.org)
Although inflammation is an important process to combat infections and to accelerate wound healing, overactivation of the immune system can lead to detrimental effects such as chronic skin inflammation 12 . (nature.com)
Wound healing proceeds through a complex collaborative process involving many types of cells. (indexindex.com)
In a wound closure seeding model, asiaticoside increased migration rates of skin cells. (indexindex.com)
Typically, these ulcers are treated by surgically transplanting existing skin to cover the wound. (singularityhub.com)
In a report in the journal Nature, we describe a new technique to directly convert the cells naturally present in an open wound into new skin cells by reprogramming the wounded cells to a stem-cell-like state , in which cells revert to an earlier, more flexible state from which they can develop into different cell types. (singularityhub.com)
Moreover, as these wounds heal, the cells multiplying in the area, known as mesenchymal cells, are involved primarily in closing the wound and inflammation, but they cannot rebuild healthy skin. (singularityhub.com)
In the wound edge, syndecan-4 was predominantly expressed by epidermal basal layer cells. (lu.se)

Obtain a skin biopsy following a thorough history and physical examination. (medscape.com)

Matrix metalloproteinase 1 overexpression has been shown to occur in both aging of skin and carcinogenesis. (medscape.com)

With its complex layers and different types of cells, skin takes a while to form, be it in a petri dish or a growing human. (alleninstitute.org)
Unlike some other types of organoids, which grow in spheres, the skin organoids sprout fleshy tentacles, Koehler said, like tiny octopi. (alleninstitute.org)
However, it is not known in which cell types CK2-mediated phosphorylation of p53 occurs. (dundee.ac.uk)
The study, published online in the journal Stem Cells, showed that this work has potential implications for treatment of optic-nerve injuries of the types incurred by soldiers in combat or athletes in contact sports. (siasat.com)
Now, a new study in Cell Stem Cell describes the mechanism behind inflammatory memory, also commonly referred to as trained immunity, and suggests that the phenomenon may be universal across diverse cell types. (scitechdaily.com)
But the molecular mechanism that explained this process, and how the cell could use it to respond to types of inflammation and injury that it had never seen before, remained a mystery. (scitechdaily.com)
Both SALPs showed a low cytotoxicity in all investigated cell types. (nature.com)
The skin comprises various cell types providing the potential to react to bacterial pathogenicity factors and can thus initiate inflammatory responses if exposed to these factors 5 . (nature.com)
This image is a cross-section of regenerated skin with different cell types indicated with fluorescent tags. (singularityhub.com)
In their latest published study, they focus on how telomerase activity affects the regenerative potential of these and other types of stem cells. (phys.org)
Telomerase is an enzyme that is essential for the self-renewal and potential of different types of stem cells. (phys.org)
There are approximately 28 different types of collagen in the human body, but four are considered the most common. (webmd.com)
Astrocytes are one of the major cell types in the central nervous system and are indispensable for brain development and function. (lu.se)
Research on the various cell types in the brain, such as astrocytes, has been hindered by difficulties in obtaining the actual cells to study. (lu.se)
Different human papillomavirus types share early natural history transitions in immunocompetent women. (who.int)

The third passage of cells was used for identification of adipogenic and osteogenic differentiation. (bvsalud.org)
The research team investigated the regenerative significance of telomerase activity , particularly in terms of characteristic cell surface marker expression, multipotent differentiation capability, chromosomal stability, and safety of in vivo formation of tumors. (phys.org)

The amino acid sequence of human skin chymase was established by protein methods and by analysis of PCR amplification products obtained with cDNA-derived from urticaria pigmentosa (UP) lesions. (duke.edu)
This sequence, along with that established for the purified protein, constituted 99% of the heart chymase primary structure, strongly indicating that human skin and heart chymases have identical primary structures. (duke.edu)

We have used a human epidermal ex vivo infection model as well as isolated LCs to investigate infection by DENV. (nih.gov)

These data identify a physiological model with which to identify signaling pathways that mediate cross-talk between p63alphaDeltaN and activating p53 kinase pathways after DNA damage in basal cell populations. (dundee.ac.uk)

however these treatments have limited efficacy and induce significant toxic effects on healthy cells. (unisalento.it)
Bacteria can release endotoxins like lipopolysaccharide (LPS) or other pathogenicity factors such as lipopoteins/peptides (LP) from their cell envelope, even due to treatment with conventional antibiotics, being able to activate Toll-like receptors (TLRs) and induce a strong inflammatory response. (nature.com)
However, they did not induce the de novo synthesis of leukotriene C 4 or prostaglandin D 2 from human basophils or any type of mast cell examined. (johnshopkins.edu)

The cytotoxicity on different cell lines was assayed using the 3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide (MTT) assay which measures survival based on the detection of mitochondrial activity and by the crystal violet assay, which measures survival based on the ability of cells to remain adherent to microplates. (scielo.br)

In May 2011, stem cell therapy in sports medicine was spotlighted after New York Yankees pitcher Bartolo Colon was revealed to have had fat and bone marrow stem cells injected into his injured elbow and shoulder while in the Dominican Republic. (cnn.com)

Clinical studies of the skin of patients with xeroderma pigmentosum indicate prominent depletion of Langerhans cells induced by UV radiation. (medscape.com)

The hair-sprouting skin organoids also developed melanocytes (pink), which are cells that produce the skin and hair's pigment, melanin. (alleninstitute.org)

Trained immunity allows cells to respond to entirely new threats by drawing on general memories of inflammation. (scitechdaily.com)
Interestingly, many of these memory domains were the same regions that had unraveled most prodigiously in the early days of skin inflammation. (scitechdaily.com)
CONCLUSIONS: The altered expression patterns of glypican and syndecan-1 and -4 in chronic ulcers reflect their possible roles during inflammation and cell proliferation. (lu.se)

Tiny clumps of skin grown from human stem cells - complete with hair follicles - represent the most lifelike human skin grown in the lab to date. (alleninstitute.org)

While previous studies of cellular aggregates derived from human stem cells could not be considered accurate human embryo models because they lacked many of the defining characteristics of a post-implementation embryo, the Weizmann synthetic embryo models had all the structures characteristic of this stage, such as the placenta and yolk sac. (israel21c.org)
A stem cell-derived human embryo model equivalent to a day-14 embryo has all the compartments that define this stage: the yolk sac (yellow) and the part that will become the embryo itself, topped by the amnion (blue) - all enveloped by cells that will become the placenta (pink). (israel21c.org)
The 14-day human embryo model under the microscope reveals the hormone used in pregnancy tests (green) and the outer layer slated to become the placenta (pink), which contains characteristic cavities, called lacunae. (israel21c.org)

Some of these were derived from reprogrammed adult skin cells and others were the progeny of lab-cultured human stem-cell lines. (israel21c.org)

When thinking about our immune system, we default to specific immunity-that cadre of T cells and B cells trained, by experience or vaccination, to remember the specific contours of the last pathogen that broke into our bodies. (scitechdaily.com)
But there's a less specific strategy available to many cells, known as trained immunity. (scitechdaily.com)
Various other defects in cell-mediated immunity have been reported in xeroderma pigmentosum. (medscape.com)
Dr. Axe: "Chicken Collagen Benefits Digestion, Immunity & Skin Health. (webmd.com)

The breakthrough follows similar success with monkey skin cells in 2007. (bigthink.com)

In 1998, President Bill Clinton requested a National Bioethics Advisory Commission to study the question of stem cell research. (cnn.com)

The study demonstrates that human primary urinary stem cells with positive telomerase activity act as a distinct subpopulation with potential regeneration capacity in both cell growth and its capacity to become other cells, Zhang said. (phys.org)

HiFive cells maintained in Grace medium between MCPyV and chimpanzee polyomaviruses, and (Invitrogen) were infected with the different recombinant between HPyV9 and simian lymphotropic polyomavirus baculoviruses for the production of the different VLPs. (cdc.gov)

Anatomy20

Organisms9

Diseases29

Chemicals and Drugs34

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes29

Disciplines and Occupations1

Technology, Industry, Agriculture2

Information Science2

Health Care4

Fibroblasts12

Keratinocytes20

Pluripotent stem5

Embryonic15

Tissue27

Cutaneous8

Vitro8

Dermal2

Mast Cells10

Dendritic3

Cancer20

Melanoma3

Keratinocyte1

Diseases12

Mesenchymal stem1

Embryos2

Differentiate4

Fragility2

Researchers11

Tissues6

Proteins3

Patient's3

Therapies3

Neurons4

Toxicity5

Biology3

Endothelial cells1

Scientists4

Grafts2

Mice6

Nucleus2

Exposure3

Squamous1

Epidermis3

Genetic4

Viability2

Functional2

Immunodeficiency virus2

Therapeutic5

Wound9

Biopsy1

Carcinogenesis1

Types15

Differentiation2

Protein2

Vivo1

Basal cell1

Induce3

Adherent1

Bone marrow1

Clinical1

Melanocytes1

Inflammation3

Grown from human1

Placenta3

Adult skin cells1

Immunity4

Monkey skin cells1

19981

Subpopulation1

Recombinant1