• 3T3 cells are several cell lines of mouse embryonic fibroblasts. (wikipedia.org)
  • In particular, we have assembled networks of Swiss 3T3 fibroblasts surrounded by a ring of bacteria. (nih.gov)
  • The present study aims to compare the proliferative effects of chitosan in two forms, membranous and solution forms, on Swiss 3T3 mouse embryonic fibroblasts. (balkanmedicaljournal.org)
  • The membranous form of chitosan induced a significant proliferative effect and increased the ratio of cell-to-cell junctions of Swiss 3T3 mouse embryonic fibroblasts. (balkanmedicaljournal.org)
  • Murine connective tissue-type mast cells, which were sensitive to mast cell secretagogues, such as compound 48/80 and substance P, were generated by co-culture of bone marrow-derived mast cells with Swiss 3T3 fibroblasts in the presence of stem cell factor. (preprints.org)
  • To investigate cell-cell interactions that may influence mammary epithelial cell differentiation, we developed a co-culture system of murine mammary epithelium and adipocytes and other fibroblasts. (silverchair.com)
  • Mammary epithelium from mid-pregnant mice was plated on confluent irradiated monolayers of 3T3-L1 cells, a subclone of the Swiss 3T3 cell line that differentiates into adipocytes in monolayer culture and other cell monolayers (3T3-C2 cells, Swiss 3T3 cells, and human foreskin fibroblasts). (silverchair.com)
  • Swiss 3T3 cells, newborn foreskin fibroblasts, substrate-attached material ("extracellular matrix"), and tissue culture plastic did not support casein synthesis, whereas monolayers of 3T3-L1 and 3T3-C2 cells, a subclone of Swiss 3T3 cells that does not undergo adipocyte differentiation, did. (silverchair.com)
  • Fig. 5: Swiss 3T3 fibroblasts stained with ActiStain 488 (green), Dapi (blue) and Anti Vinuclin (orange). (tebubio.com)
  • We studied whether cellular senescence induced by HDAC inhibitor sodium butyrate in E1a+cHa-Ras -transformed rat embryo fibroblasts (ERas) and A549 human Ki-Ras mutated lung adenocarcinoma cells would enhance the tumor suppressor effect of MEK/ERK inhibition. (aging-us.com)
  • Go¨6976 also induced laminin-dependent cell adhesion in NIH/3T3 and CV-1 fibroblasts, suggesting of a mechanism that may be common to multiple cell-types. (mirnamimic.com)
  • The application of hydrogels as a matrix for 3-dimensional cell cultures has become an indispensable tool in tissue engineering, biotechnology and biomedical research due to the improved functionality and viability of the in vitro biological system. (heraldopenaccess.us)
  • On the other hand, even if 3D aggregate culture methods are able to recapitulate in vitro the cell-extracellular matrix interactions properly observed in vivo, and the synthetic/natural matrix and scaffolds have biochemical and mechanical properties, in order to mimic the native extracellular matrix, both of these systems possesses some limitations and some methodological improvements are needed. (razavihospital.ir)
  • Results: The processes by which re-aggregated adult single cell types or adult and embryonic explanted tissues are able to recapitulate embryogenesis in vitro, when cultured in adhesion or embedded in 3D gels, is not surprising and is clearly under the control of a reminiscent cellular memory which recapitulates early developmental stages. (razavihospital.ir)
  • Moscona A. The Development in Vitro of Chimeric Aggregates of Dissociated Embryonic Chick and Mouse Cells. (razavihospital.ir)
  • Kurosawa H. Methods for inducing embryoid body formation: in vitro differentiation system of embryonic stem cells. (razavihospital.ir)
  • Culture and differentiation of preadipocytes in two-dimensional and three-dimensional in vitro systems. (razavihospital.ir)
  • In vitro characterization and delivery of chitosan-DNA microparticles into mammalian cells. (ualberta.ca)
  • In the present study, we have investigated DNA encapsulation efficiency and loading capacity of chitosan microparticles prepared in different conditions, as well as in vitro DNA release from microparticles, and transfection of different cell lines with chitosan-DNA microparticles, which may be employed in future in vivo studies. (ualberta.ca)
  • Although the transfection efficiency of chitosan-DNA microparticles was typically lower than that of DNA complexed with lipid-based reagents, in vitro transfection results indicated that HEK293 cells take up chitosan-DNA microparticles more efficiently compared to HeLa and mouse fibroblastic 3T3 cell lines. (ualberta.ca)
  • The efficient delivery of functional therapeutic genes into target cells in vitro and in vivo is an important problem in gene therapy approaches for the treatment of hereditary diseases and cancer (9, 10). (ualberta.ca)
  • Chitosan microparticles containing reporter genes are being used for the transfection of mammalian cells both in vitro and in vivo conditions (28-30). (ualberta.ca)
  • 2000. Results of the L5178Y mouse lymphoma assay and the Balb/3T3 cell in vitro transformation assay for eight phthalate esters. (cdc.gov)
  • The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION. (jefferson.edu)
  • Advances in microfabrication, stem cell technology, tissue engineering, and mathematical modeling have created unprecedented in vitro models-organs-on-a-chip (OOC) systems-to predict risk and efficacy of drugs candidates, and accelerate their steps to the market. (mdpi.com)
  • In primary cultures of mouse embryonic day 14 (E14) striatum, maintained in vitro for 24 hr, 12% of the cells responded to FGF-2, whereas no response to EGF could be detected. (beauty104.com.tw)
  • Priming of striatal cells for 24 hr with FGF-2 but not with EGF was sufficient to induce the appearance of EGF- and FGF-2 responsive cells after only 2 d in vitro. (beauty104.com.tw)
  • Thus, neural precursor cells from the mouse E14 striatum initially responding to FGF-2 only acquire EGF responsiveness later during in vitro development. (beauty104.com.tw)
  • The most efficient method to expand limbal stem cells (LSCs) in vitro for clinical transplantation is to culture single LSCs directly on growth-arrested mouse fibroblast 3T3 cells. (drcremers.com)
  • To reduce possible xenobiotic contamination from 3T3s, primary human adipose-derived stem cells (ASCs) were examined as feeder cells to support the expansion of LSCs in vitro . (drcremers.com)
  • Ésta línea es un sistema hospedador in vitro de gran valor para los estudios de transformación de virus oncogénicos, ya que las células 3T3 poseen una elevada sensibilidad a la inhibición por contacto. (bvsalud.org)
  • RESEARCH DESIGN AND METHODS AND RESULTS- First, we examined whether the PPARβ/δ agonist GW501516 prevents lipopolysaccharide (LPS)-induced cytokine production in differentiated 3T3-L1 adipocytes. (diabetesjournals.org)
  • To test for this, apelin-55 was applied exogenously to HEK293A cells overexpressing proprotein convertase subtilisin kexin 3 (PCSK3), the only apelin processing enzyme identified thus far, and to differentiated 3T3-L1 adipocytes, which endogenously express apelin, PCSK3 and other proprotein convertases. (phoenixpeptide.com)
  • Adherent cells grow as a monolayer. (wikipedia.org)
  • A confluent monolayer yields 40000 cells/cm2. (wikipedia.org)
  • Contact inhibited, a confluent monolayer of 3T3 cells yields approximately 40,000 cells per square centimeter. (fsu.edu)
  • Above: Swiss3T3 cell monolayer labeled with LipiBright™ SMCy3.5. (cytoskeleton.com)
  • It has been repeatedly demonstrated that the presence of a 3D matrix promotes many biologically relevant functions otherwise not observed in 2D monolayer cell cultures [11]. (heraldopenaccess.us)
  • HeLa (human cervical carcinoma), DLD-1 (human colon adenocarcinoma) and Balb/c 3T3 (Swiss mouse embryo) cell lines (purchased from ATCC, Manassas, VA) and HUVEC (human umbilical vein endothelial cells, bought from Cambrex, Walkersville, VA) had been also employed. (estrogen-receptor.com)
  • At the time of their establishment, 3T3 cells were different than most other cell lines in regard to the fact they did not induce tumors to develop when injected into murine species. (fsu.edu)
  • Studies showing that murine fibroblast cell lines are susceptible to infection with mouse-adapted scrapie agent ( 11 , 12 ) increased concern that nonneuronal cell substrates used to propagate viruses for vaccine production might become infected with a TSE agent contaminating some component of culture medium, especially bovine serum ( 13 ). (cdc.gov)
  • Comparison of the resulting apelin-55-derived peptide profile between the two cell lines demonstrated distinct processing patterns, with apelin-36 production apparent in 3T3-L1 adipocytes vs. detection of the prodomain of a shorter isoform (likely the apelin-13 prodomain, observed after additional proteolytic processing) in PCSK3-transfected HEK293A cells. (phoenixpeptide.com)
  • The original 3T3 cell line (3T3-Swiss albino) was established in 1962 by two scientists then at the Department of Pathology in the New York University School of Medicine, George Todaro and Howard Green. (wikipedia.org)
  • Todaro and Green originally obtained their 3T3 cells from Swiss albino mouse embryo tissue. (wikipedia.org)
  • One of the most important functions of bFGF is the promotion of endothelial cell proliferation and the physical organization of endothelial cells into tube-like structures. (beauty104.com.tw)
  • BNP treatment increased vascularisation and the number of endothelial cells in all areas of infarcted hearts. (elifesciences.org)
  • Endothelial cell lineage tracing showed that BNP directly stimulated the proliferation of resident endothelial cells via NPR-A binding and p38 MAP kinase activation. (elifesciences.org)
  • Our results demonstrated that these immature cells have a natural capacity to differentiate into endothelial cells in infarcted hearts. (elifesciences.org)
  • The origin of new endothelial cells (i.e. resident or infiltrating) as well as the underlying mechanism leading to their proliferation (partial endothelial-to-mesenchymal transition [EndMT] or not) have long been debated. (elifesciences.org)
  • One complementary therapy could be 're-activating' vasculogenesis (i.e. the differentiation of precursor cells into mature endothelial cells), a mechanism that occurs in the heart during development but is quiescent in adult hearts. (elifesciences.org)
  • Established by George Todaro and Howard Green in 1962 from disaggregated Swiss mouse ( Mus musculus ) embryo tissue, the 3T3 cell line is a standard fibroblast cell line used in a wide spectrum of research and industrial biomedical applications. (fsu.edu)
  • LipiBright™ should be reconstituted with 200 µl of anhydrous DMSO to create a 340 µM stock solution for cell imaging or with 40 µl of anhydrous DMSO to create a 1.5 mM stock suitable for tissue or small organism imaging. (cytoskeleton.com)
  • This is the first time to our knowledge that living cell arrays of such complexity have been synthesized, and it represents a milestone in synthetic biology and tissue engineering. (nih.gov)
  • As the solution form is easy to prepare and apply to cells compared to the membrane form, the application of Chitosan directly to media appears to be a convenient alternative for tissue engineering approaches. (balkanmedicaljournal.org)
  • The main advantage of hydrogels for microfluidic cell cultures, however, is their mimicry of extracellular matrix structures including adequate porosity for cellular organization, biocompatibility, and representative stiffness, all key parameters that promote native-like tissue function. (heraldopenaccess.us)
  • It has become clear, however, that the susceptibility of cells to infection with TSE agents cannot be reliably predicted either from their tissue of origin or level of expression of PrP C ( 11 , 12 ). (cdc.gov)
  • Tumor cell invasion and metastasis require cancer cells to be both proliferative and invasive, i.e. migrate through the tissue and assemble invadopodia. (biorxiv.org)
  • Liposome-mediated transfection of intact viral particles reveals that plasma membrane penetration determines permissivity of tissue culture cells to rotavirus. (microbiologyresearch.org)
  • Stem cells are known to work via a variety of methods to heal tissue depending on where the stem cells come from and into which tissue it is being placed. (drcremers.com)
  • Here is a general list of all the Ways Stem Cells heal tissues or create new tissue. (drcremers.com)
  • 1. Stem cells have long been known to have the ability to differentiate (ie become/transform) into "good/normal" tissue. (drcremers.com)
  • Embryonic stem cells have been fraught with difficulties in coaxing them into "good/normal" tissue and there are major ethical issues. (drcremers.com)
  • Synthetic peptides or recombinant fragments containing bio- logically active motifs have been shown to partially mimic the effects of native laminin in various cellular functions, such as cell adhesion, spreading, migration, neurite outgrowth, and tumor metastasis [6-10], suggesting that laminin-derived-short peptide- modified biomaterials may have therapeutic potential in tissue regeneration applications. (mirnamimic.com)
  • Inhibiting Rho blocks FN matrix set up, whereas presenting recombinant, constitutively energetic Rho into quiescent Swiss 3T3 cells promotes matrix set up by these cells. (geogise.com)
  • Is 5,11,14 20:3 taken up by cultured human keratinocyte PL, can it displace 20:4n-6 from PL pools and reduce PGE 2 levels, and does it affect tumor necrosis factor α(TNFα) production? (biomedcentral.com)
  • Despite recent achievements of microfluidic 2D cell culture systems [9], they still do not address the fact that in vivo cells coexist in 3D communities that are influenced by spatial orientation of cells and cell-to-cell contact within the extracellular matrix [10]. (heraldopenaccess.us)
  • Metastasis is a complex, multi-step process that is initiated when cancer cells in the primary tumor acquire invasive properties, including motility and the ability to breakdown the extracellular matrix (ECM) 1 , and is responsible for the majority of cancer-related mortalities 2 . (biorxiv.org)
  • Fibronectin (FN)1 is normally a big, multi-module extracellular matrix (ECM) protein that is available in two main state governments, either circulating in plasma being a soluble dimeric protein or present within ECMs as an insoluble element connected with cells and various other ECM elements. (geogise.com)
  • J. Cell Biol. (wikipedia.org)
  • J Cell Biol (1985) 100 (5): 1415-1422. (silverchair.com)
  • This image is the original data file from Fig. 2F "colocalization of MII with GEFs", J. Cell Biol. (ucsd.edu)
  • Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). (jefferson.edu)
  • Int J Biochem Cell Biol. (jefferson.edu)
  • Cellular morphology, time-lapse imaging, and nuclear staining demonstrated that this activity occurred in mitotically active cells. (jneurosci.org)
  • CC-ASC generated epithelial cells with undifferentiated morphology in all culture methods, among which CC-ASC in 3D culture supported the highest cell doubling (cells doubled 9.0 times compared to cells doubled 4.9 times in control) while maintained the percentage of putative limbal stem/progenitor cells compared to the control. (drcremers.com)
  • Conveniently, the solution form also resulted in enhanced cell proliferation and viability compared to the control group. (balkanmedicaljournal.org)
  • Low power laser irradiation (LPLI) promotes proliferation of multiple cells, which (especially red and near infrared light) is mainly through the activation of mitochondrial respiratory chain and the initiation of cellular signaling. (biomedcentral.com)
  • Cell proliferation is a very important physiological effect for low power laser irradiation (LPLI) used in clinical practice. (biomedcentral.com)
  • However, the mechanisms of cell proliferation induced by LPLI are poorly understood. (biomedcentral.com)
  • Recently, a large number of signaling proteins reported play an important key role in the process of LPLI-induced cell proliferation, probably due to the fact that the molecular events they are involved in are the basic response of the cells to extracellular stimuli. (biomedcentral.com)
  • Epidermal growth factor (EGF) and fibroblast growth factor 2 (FGF-2) induce the proliferation of neural precursor cells isolated from specific regions of the embryonic and adult brain. (beauty104.com.tw)
  • Not long after FGF1 and FGF2 were isolated, another group isolated a pair of heparin -binding growth factors which they named HBGF-1 and HBGF-2, whilst a third group isolated a pair of growth factors that caused proliferation of cells in a bioassay containing blood vessel endothelium cells which they called ECGF-1 and ECGF-2. (beauty104.com.tw)
  • BNP also stimulated the proliferation of WT1 + epicardium-derived cells but only in the hypoxic area of infarcted hearts. (elifesciences.org)
  • In colorectal cell lines, TGF B induces proliferation by RAS independent manner. (microrna1.com)
  • To study the molecular mechanism by which nonmuscle myosin II (MII) regulates protrusion and adhesion dynamics in migrating cells, Swiss3T3 cells were co-stained for βPIX (green) and myosin IIB (red). (ucsd.edu)
  • These findings help elucidate a functional link between MII and Rac1/Cdc42 GTPases, which may regulate protrusion/adhesion dynamics in migrating cells. (ucsd.edu)
  • In experiments probing for a role of PKCa in human laminin-2-integrin-mediated cell adhesion and spreading of PC12 cells, we observed unexpected enhancements of adhesion, spreading and stress fiber formation to 1 mM Go¨6976 with concomitant increase in membrane translocation of PKCd and autophosphorylation of focal adhesion kinase (FAK). (mirnamimic.com)
  • A specific inhibitor of PKCd, rottlerin, completely abrogated Go¨6976-dependent increase in PC12 cell adhesion to laminin as well as the activation of small GTPases, Rac1 and Cdc42, that are downstream of PKCd in adhesion receptor signaling. (mirnamimic.com)
  • Biological activities of laminin are mediated through binding to a subset of integrin family of cell adhesion receptors, a3b1, a6b1, a6b4, and a7b1 [2]. (mirnamimic.com)
  • PKC activation by phorbol myristate acetate has been shown to induce the adhesion, spreading, and migration of cells [12]. (mirnamimic.com)
  • Pharmacologic blockade of PKC inhibits cell adhesion and spreading as well as focal adhesion formation and focal adhesion kinase (FAK) phosphorylation [13,14]. (mirnamimic.com)
  • In preliminary experiments examining the role of PKCa in cell adhesion and spreading, we found that Go¨6976, a widely used, nonglycosidic indolocarbazole inhibitor of PKCa/b, had effects that could not be explained by inhibition of PKCa/b alone. (mirnamimic.com)
  • 3T3 cells can be transformed with SV40 and some other polyomaviruses. (wikipedia.org)
  • Variants of the initial cell line have been tested and found negative for ectromelia virus (mousepox), but most are susceptible to polyoma and simian virus 40 ( SV40 ). (fsu.edu)
  • However, due to examination of 3T3 cells and subsequent research it has become widely accepted that for immortalization of cells to take place, telomere shortening, which can instigate chromosomal rearrangements, must be overcome, a process that is not necessarily related to a cell s ability to undergo oncogenic transformation. (fsu.edu)
  • The isolated 3T3 cell presented in the digital image above was resident in an adherent culture stained for F-actin with Alexa Fluor 568 conjugated to phalloidin, and for DNA with 4',6-diamidino-2-phenylindole ( DAPI ). (fsu.edu)
  • Swiss Mouse Embryo Fibroblast Cells with MitoTracker Red CMXRos, Alexa Fluor 488, and DAPI - A log phase culture of embryonic Swiss mouse fibroblast cells was stained with MitoTracker Red CMXRos, Alexa Fluor 488 conjugated to phalloidin, and DAPI, which target the intracellular mitochondrial network, cytoskeletal actin filaments, and nuclei, respectively. (fsu.edu)
  • Tubulin, Actin, and DNA Distribution in 3T3 Cells - A culture of 3T3 cells was immunofluorescently labeled with primary anti-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to the cyanine dye, Cy3. (fsu.edu)
  • In addition, the cells were simultaneously probed for DNA with the ultraviolet-absorbing probe DAPI, and for the cytoskeletal filamentous actin network with Alexa Fluor 488 conjugated to phalloidin. (fsu.edu)
  • Wide-field fluorescent imaging of live Swiss3T3 labeled with LipiBright™ SMCy3.5 (Pink), a live cell actin cytoskeleton stain (Green, SiR-Actin), and a live cell DNA probe (Blue, SPY505-DNA). (cytoskeleton.com)
  • Fig. 1: Immunofluorescence images of mouse Swiss 3T3 cells stained with anti-actin antibody (027AAN01). (tebubio.com)
  • Actin and Tubulin, as the major cytoskeleton structures, are crucial components of a plethora of processes in cell biology. (tebubio.com)
  • Thus visualizing Actin and Tubulin in fixed or living cells and detecting them in biological samples (e.g. in Western Blots) belongs to basic experimental set-ups in Cell Biology. (tebubio.com)
  • Fig. 2: Western blot of purified actin and cell extracts probed with anti-actin antibody (027AAN01). (tebubio.com)
  • In fixed cells Actin can be stained with antibodies (see above) but often fluorescent phalloidins are used. (tebubio.com)
  • The natural toxicity of Phalloidin is due to its stabilizing effect on F actin in cells. (tebubio.com)
  • Finally, I would like to introduce brand new tools to stain Actin and Tubulin in living cells without the need to transfect them with vectors carrying the information for fluorescently labeled Actin/Tubulin or binding proteins. (tebubio.com)
  • I have routinely used fluorescently conjugated phalloidin to stain actin cytoskeletal elements for years, in fixed, permeabilized cells. (tebubio.com)
  • Purified 5,11,14 20:3 was taken up by cultured human skin keratinocytes, reduced 20:4n-6, and reduced PGE 2 levels dramatically. (biomedcentral.com)
  • While several studies addressed how cell motility parameters change throughout the cell cycle, the relationship between invadopodia and cell cycle progression has not been elucidated. (biorxiv.org)
  • In adult Swiss Webster female mice fed technical captan in their diet at levels ranging from 4 000 to 16 000 ppm for up to 35 days, soluble thiol levels in the duodenum from the proximal end were elevated throughout the feeding period. (inchem.org)
  • Shih CC, Forman SJ, Chu P, Slovak M. Human embryonic stem cells are prone to generate primitive, undifferentiated tumors in engrafted human fetal tissues in severe combined immunodeficient mice. (razavihospital.ir)
  • branching in 3D cultures, an occasion that correlated with enhanced pulmonary outgrowth of breast cancer cells in mice. (microrna1.com)
  • OR cell inocu lated into the lateral tail veins of mice to stay dormant from the lungs for a span of up to five wk. (microrna1.com)
  • In this study, using invadopodia- and cell cycle- fluorescent markers, we show in 2D and 3D cell cultures, as well as in vivo , that breast carcinoma cells assemble invadopodia and invade into the surrounding ECM preferentially during the G1 phase of the cell cycle. (biorxiv.org)
  • Here we show by in vivo fate mapping that brown, but not white, fat cells arise from precursors that express Myf5, a gene previously thought to be expressed only in the myogenic lineage. (bioseek.eu)
  • Visualizing Structural Features of the Golgi Complex and Nucleus in Swiss Mouse Embryo Cells - In order to examine structural features of the Golgi complex and nucleus at relatively high magnification, a log-phase culture of 3T3 cells was fixed, permeabilized, blocked with normal goat serum, and then treated with rabbit anti-giantin (Golgi protein) primary antibodies followed by goat anti-rabbit secondary antibodies (IgG) conjugated to Alexa Fluor 568. (fsu.edu)
  • Synthesis and accumulation of alpha-, beta-, and gamma-mouse casein within the epithelium was shown by immunohistochemical staining of cultured cells with anti-casein monoclonal antibodies, and the specificity of the immunohistochemical reaction was demonstrated using immunoblots. (silverchair.com)
  • The LipiBright™ probes consists of bright, fluorogenic live cell dyes that stain lipid droplets. (cytoskeleton.com)
  • The formation of new vessels in the hypoxic area restores blood flow, provides oxygen and nutriments to the surviving cells, and promotes the migration and engraftment of new cells. (elifesciences.org)
  • During this time, precursor cells undergo interkinetic nuclear migration ( Seymour and Berry, 1975 ) in which cells in the DNA synthetic S phase have their nuclei in the upper third of the VZ. (jneurosci.org)
  • 3T3 Cells" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (jefferson.edu)
  • Prominent collagen synthesis was detected in the membrane groups on the 10th day of culture for both methods. (balkanmedicaljournal.org)
  • There was evidence of specificity in the cell-cell interaction that mediates hormone-dependent casein synthesis. (silverchair.com)
  • PROPERTIES: the cells contain the transforming gene neu which induces synthesis of a tumor antigen of relative molecular mass 185,000 (p185), strongly associated with the presence of neuro/glioblastoma oncogenes. (hsanmartino.it)
  • Identification, synthesis, and modifications of simian rotavirus SA11 polypeptides in infected cells. (microbiologyresearch.org)
  • Modulation of intracellular free calcium concentration ([Ca 2+ ] i ) may be part of the signaling pathway by which both local environmental factors and cell autonomous developmental programs influence corticogenesis. (jneurosci.org)
  • The successful strategy based on the activation of cell death in Ras-expressing cells is associated with the suppression of kinases involved in Ras pathway. (aging-us.com)
  • Senescent ERas cells do not develop cytoprotective autophagy upon inhibition of MEK/ERK pathway due to spatial dissociation of lysosomes and autophagosomes in the senescent cells. (aging-us.com)
  • Our data show that suppression of MEK/ERK pathway in senescent cells provides a new strategy for elimination of Ras-expressing cells. (aging-us.com)
  • Despite a large number of promising inhibitors for Ras/Raf/MEK/ERK pathway, recent works show that cancer cells often develop an autophagy-dependent resistance to inhibitors of Ras pathway [ 16 ]. (aging-us.com)
  • We previously demonstrated that invasive cells oscillate between two distinct states, termed the Invadopodia state and the Migration state 10 . (biorxiv.org)
  • The Invadopodia state is characterized by cell stasis and the presence of invadopodia, whereas the Migration state is defined by cell translocation and the absence of invadopodia. (biorxiv.org)
  • Both are very much involved in stabilizing the cell shape, and especially in cell movements (e.g. cell migration) and intracellular movements and transport mechanisms. (tebubio.com)
  • The primary mouse embryonic fibroblast cells were transferred (the "T") every 3 days (the first "3"), and inoculated at the rigid density of 3×105 cells per 20 cm2 dish (the second "3") continuously. (wikipedia.org)
  • Mouse Embryo Fibroblast Cells with Texas Red-X, Alexa Fluor 488, and DAPI - In this section, a Swiss mouse embryo fibroblast cell is presented that was resident in a culture labeled with the fluorophore Texas Red-X conjugated to wheat germ agglutinin, a fluorescent lectin that selectively binds to sialic acid residues. (fsu.edu)
  • Immunohistochemical staining confirmed that these events occurred primarily in precursor cells rather than in postmitotic neurons. (jneurosci.org)
  • The morphological characteristics of these cells and immunohistochemical staining suggested that the coordinated events occurred in gap junction-coupled precursor cells. (jneurosci.org)
  • Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. (razavihospital.ir)
  • Although spontaneous changes of [Ca 2+ ] i have been examined in immature cortical neurons, the calcium dynamics of cortical precursor cells have received less attention. (jneurosci.org)
  • These results demonstrate distinct patterns of spontaneous [Ca 2+ ] i change in cortical precursor cells and raise the possibility that these dynamics may contribute to the regulation of neurogenesis. (jneurosci.org)
  • Both intrinsic and extrinsic signals are likely to influence the proliferative potential and eventual fates of precursor cells within the VZ. (jneurosci.org)
  • With high GM values (2400 to 13300), the LipiBright SMCy3.5 and SMCy5.5 probes have been confirmed to work with two photon microscopy to work in multiple models including cells and tissues 1 . (cytoskeleton.com)
  • The main benefit of microfluidic systems for cell culture analysis is that they can perform a number of crucial liquid handling steps including cell loading, nutrient supply and waste removal under physiologically relevant shear force conditions, all while offering real time microscopy [4]. (heraldopenaccess.us)
  • Using an intact cortical mantle and confocal laser microscopy, we examined the spatiotemporal patterns of spontaneous [Ca 2+ ] i fluctuations in neocortical ventricular zone (VZ) cells in situ . (jneurosci.org)
  • Moreover, knockdown of FAAH by siRNA was shown to confer decreased cancer cell invasiveness and increased TIMP-1 expression. (oncotarget.com)
  • Outgrowth proficient cells lack E cad expression Given the differential necessity of EMT to boost the pulmo nary outgrowth of NM E cells and never that of D2. (microrna1.com)
  • A1 cells readily up regu lated the expression of three integrin in response to TGF, a molecule we established as one particular on the most delicate and robust markers of TGF signaling. (microrna1.com)
  • OR cells failed to down regulate their expression of E cad. (microrna1.com)
  • Inclusion of the R I antagonist, SB431542, to these cultures resulted in the dramatic diminution of E cad expression. (microrna1.com)
  • OR cells to down regulate E cad expression as a part of their EMT plan, a deficit that may underlie their preferential acquisi tion of dormant phenotypes during pulmonary metastasis. (microrna1.com)
  • Importantly, enhanced cellular behavior and membrane translocation of PKCd induced by Go¨6976 was retained in siRNA-transfected PC12 cells to knockdown PKCa expression. (mirnamimic.com)
  • siRNA knockdown of Rac1 and Cdc42 expression inhibited cell spreading and lamellipodia formation in PC12 cells. (mirnamimic.com)
  • Later, as a principal investigator position at the National Cancer Institute in Bethesda, Maryland, Todaro repeated the isolation procedure from the NIH Swiss mouse embryo with his students and established NIH-3T3 cell line. (wikipedia.org)
  • previous to studies of the 3T3 line, it was believed that these characteristics were necessarily synonymous with one another. (fsu.edu)
  • No PrP TSE or infectivity has been detected in any exposed cell line under study so far. (cdc.gov)
  • Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines. (hsanmartino.it)
  • This is the complete description of the cell line that you selected. (hsanmartino.it)
  • Cell line available for distribution. (hsanmartino.it)
  • Proapelin is processed extracellularly in a cell line-dependent manner with clear modulation by proprotein convertases. (phoenixpeptide.com)
  • The intent of vaccination is to induce a combined antibody and T-cell anti-HER-2 immune response, which is intended to target HER-2-expressing tumor cells, and may induce tumor regression or slow progression of disease. (stanford.edu)
  • The primary purpose of this study is to determine whether breast cancer tumors respond (as measured by pathologic complete response: the absence of microscopic evidence of invasive tumor cells in the breast) to combined chemotherapy of AC(doxorubicin and cyclophosphamide) followed by paclitaxel plus trastuzumab or lapatinib or both given before surgery to patients with HER2-positive breast cancer. (stanford.edu)
  • RATIONALE: Drugs used in chemotherapy use different ways to stop tumor cells from dividing so they stop growing or die. (stanford.edu)
  • However, under these microenvironmental conditions, only 15% of tumor cells assemble invadopodia at any given time, while the rest are non-motile. (biorxiv.org)
  • In recent years, cell cycle regulators have been shown to exhibit roles in both tumor suppression and tumor promotion, particularly cyclin-dependent kinase inhibitors (CKI) p27 kip1 and p21 cip1 14 . (biorxiv.org)
  • Chemotaxis of metastatic tumor cells: Clues to mechanisms from the Dictyostelium paradigm. (godman-inc.com)
  • This can provide Ras-expressing tumor cells additional selective advantages under stress or damage. (aging-us.com)
  • The macrophageregulator GM-CSF, which can be made by some types of cancer cells [18,19], particularly induces HB-EGF in macrophages and neutrophils [20]. (estrogen-receptor.com)
  • Feeder layers: co-culture with nonneoplastic cells. (jefferson.edu)
  • To optimize the ASC-supported culture, freshly isolated limbal epithelial cells in the form of single cells (SC-ASC) or cell clusters (CC-ASC) were cultured using three different methods: LSCs seeded directly on feeder cells, a 3-dimensional (3D) culture system and a 3D culture system with fibrin (fibrin 3D). (drcremers.com)
  • The standard method to culture LSCs on 3T3 feeder cells that have been used in clinical study is cultivating single LSC directly on top of the growth-arrested 3T3 feeder cells [ 14 ]. (drcremers.com)
  • This study addressed the impact of two FAAH inhibitors (arachidonoyl serotonin [AA-5HT], URB597) on A549 lung cancer cell metastasis and invasion. (oncotarget.com)
  • Fig. 3: Immunofluorescence image of Arabidopsis thaliana metaphase cell stained with Anti-Tubulin polyclonal antibody (027ATN02). (tebubio.com)
  • Are there similar probes to be used as non-antibody stains of tubulin cytoskeletal elements in fixed, permeabilized cells? (tebubio.com)
  • Our SIR tubulin probes are meant for live cell imaging. (tebubio.com)
  • Seventy-five percent of these FGF-2-responsive cells were beta tubulin III (TuJ1)-positive neurons, and 25% expressed nestin, a marker for neuroepithelial precursors. (beauty104.com.tw)
  • We have assembled three-dimensional heterotypic networks of living cells in hydrogel without loss of viability using arrays of time-multiplexed, holographic optical traps. (nih.gov)
  • Cell-free supernatants (SN) had been stored at -80 .Immunochemistry on tissues and cellsMethodsEthical requirementsThe blood and histological samples applied in our study have been in compliance with Institutional Review Board regulations.Cells and reagentsHistological samples were obtained by hepatic lobectomy to excise metastatic nodules derived from colon cancer. (estrogen-receptor.com)
  • The light must be absorbed by the endogenous chromophores of cells or tissues for actions. (biomedcentral.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • The epidemic of obesity, closely associated with increases in diabetes, hypertension, hyperlipidemia, cancer and other disorders, has propelled a major interest in adipose cells and tissues. (bioseek.eu)
  • Adipose tissues contain two distinct types of fat cells, white and brown. (bioseek.eu)
  • OR cells, we up coming sought to confirm the response of D2 HAN derivatives to TGF by characterizing a repertoire of target genes recognized to be regulated by this selelck kinase inhibitor multi practical cytokine. (microrna1.com)
  • The objective of this study was to determine the potential susceptibility to infection of 5 cell lines used or proposed for manufacture of biological products, as well as other lines. (cdc.gov)
  • Cell lines were exposed to the infectious agents of sporadic and variant Creutzfeldt-Jakob disease and bovine spongiform encephalopathy (BSE). (cdc.gov)
  • All cell lines we studied resisted infection with 3 TSE agents, including the BSE agent. (cdc.gov)
  • SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines). (hsanmartino.it)
  • SMAD3 mutation is often a incredibly unusual occasion in human solid tumors, nonetheless, a missense mutation in SMAD3 gene was found in human colorectal cell lines. (microrna1.com)
  • Proteomic screen of SMAD4 TAK-960 wt and SMAD4 deficient cell lines detected distinctive protein levels in cell lines pointing to SMAD4 dependent and independent TGF B responses in colon carcinoma cells. (microrna1.com)
  • This decrease was partially, but not fully, attenuated by PCSK inhibitor treatment in both cell lines. (phoenixpeptide.com)
  • Exposed cultures were tested for TSE-associated prion protein (PrP TSE ) and TSE infectivity by assay in rodents and nonhuman primates. (cdc.gov)
  • Mei H, González S, Nakatsu MN, Baclagon ER, Chen FV, Deng SX (2017) Human adipose-derived stem cells support the growth of limbal stem/progenitor cells. (drcremers.com)
  • In some experiments, the conditioned medium was replaced with fresh medium just after 24 hours of stimulation and the cells were then maintained in culture for up to 48 hours. (estrogen-receptor.com)
  • More recent research applications include microchips for genomics, proteomics and cell-based assays. (heraldopenaccess.us)
  • Soon after growing in cultures for the proper instances in unique conditions, the cells have been either lysed for total RNA extraction or utilized for functional assays. (estrogen-receptor.com)
  • These methods involve the use of genetically modified viruses to deliver the genetic material (11, 12), non-viral gene transfers including the direct physical introduction of the genetic material into cells (13-15) and formation of DNA complexes with inorganic salts, polycations, or lipids to transfer the DNA into the cells (16-18). (ualberta.ca)
  • In PC12 cells, we recently showed that the engagement of integrin a3b1 to a binding sequence within the human laminin a2 chain was sufficient to recruit PKCa and PKCd to the integrin receptor [6]. (mirnamimic.com)
  • High signal levels from all three of the fluorophores employed to stain the culture are present. (fsu.edu)
  • When it's utilized to stain regular cultures that are developing stress, a matrix is revealed because of it indistinguishable from that revealed by polyclonal anti-fibronectin antibodies. (geogise.com)
  • The combination of 3-dimensional hydrogel cell cultures with microchip technology further allows (i) spatial and temporal control of cell growth, (ii) application of defined mechanical (e.g. shear, strain, stretch) and chemical (e.g. gradients) stimuli, as well as (iii) monitoring of dynamic cellular responses using integrated sensing strategies. (heraldopenaccess.us)
  • This review focuses primarily on recent advances in biologically inspired microfluidic systems that are based on 3-dimensional hydrogel cell cultures and discusses advantages and current challenges, various applications of microfluidic hydrogel cell cultures and future perspectives. (heraldopenaccess.us)
  • In addition, the culture was immunofluorescently labeled with Cy2 conjugated to antibodies that target peroxisomal membrane protein 70 ( PMP 70 ), an abundant and integral membrane component of peroxisomes. (fsu.edu)
  • The serine/threonine kinase protein kinase D1 (PKD1) is a stress-responsive kinase and sensor for reactive oxygen species, which can initiate cell survival through NF-κB signaling. (biologists.com)
  • In this study we used phosphorylation of the transcription factor cAMP response element-binding protein as a functional readout to identify cells responding to EGF and FGF-2. (beauty104.com.tw)
  • OR cells with TGF truly enhanced the ranges of E cad mRNA and protein. (microrna1.com)
  • MAPKs are Serine-threonine protein kinases that are activated in response to a variety of extracellular stimuli, and mediate signal transduction from the cell surface to the nucleus. (thermofisher.com)
  • Komura H, Ogita H, Ikeda W, Mizoguchi A, Miyoshi J, Takai Y. Establishment of cell polarity by afadin during the formation of embryoid bodies. (razavihospital.ir)