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  • proliferation
  • No significant differences in cell surface expression of leukocyte markers, in efficiency of selection of PBMC subpopulations, or in mitogen-induced proliferation were detected in freshly isolated or cryopreserved cells. (duke.edu)
  • PBMC
  • 95% depletion of monocytes from PBMC following immunomagnetic selection with anti-CD14. (duke.edu)
  • No significant differences in cell surface expression of leukocyte markers, in efficiency of selection of PBMC subpopulations, or in mitogen-induced proliferation were detected in freshly isolated or cryopreserved cells. (duke.edu)
  • clinical
  • The currently available techniques of magnetic-bead-based enrichment and subsequent phenotyping analysis of rare tumor cells from clinical samples facilitate their detailed characterization. (biomedcentral.com)
  • However, studies of tlie action of these cytokines on hemopoietic stem cells are important research fields in regarding to clinical uses such as in votro expansion for transplantation. (nii.ac.jp)
  • Highly purified autologous CD133+ cells can stimulate neo-angiogenesis, as based on clinical and CEUS data. (biomedcentral.com)
  • Basal cell carcinoma and squamous cell carcinoma are common entities in clinical practice. (biomedsearch.com)
  • receptor
  • NKT cells, and is associated with the T cell receptor. (miltenyibiotec.com)
  • Th17-derived Th1 lymphocytes, termed nonclassic, differ from classic Th1 cells because of the presence of retinoic acid orphan receptor (ROR)C2 and the surface expression of CD161 and CCR6. (jimmunol.org)
  • Expression and function of the P2X(7) receptor in rat C6 glioma cells. (biomedsearch.com)
  • Our results demonstrate the first findings of expression and function of the purinergic P2X7 receptor (P2X7R) in rat C6 glioma cells. (biomedsearch.com)
  • carcinoma
  • Buffy coat cells spiked with varying numbers of BT-474 carcinoma cells were permeabilized and fixed, following which carcinoma cells were magnetically labelled with an anti-cytokeratin 8 mAb. (biomedsearch.com)
  • MCSP is expressed on the majority (>90%) of human melanoma tissues and melanoma cell lines but not on carcinoma cells, fibroblastoid cells, and cells of hematopoietic origin. (miltenyibiotec.com)
  • Even though immunohistochemical comparisons of microcystic adnexal carcinoma vs infiltrative basal cell carcinoma and desmoplastic trichoepithelioma exist, they are mostly restricted to the use of a single stain. (biomedsearch.com)
  • In addition, a comparison with squamous cell carcinoma has not been reported previously. (biomedsearch.com)
  • CD-10 immunostaining differentiates superficial basal cell carcinoma from cutaneous squamous cell carcinoma. (biomedsearch.com)
  • Orbital metastasis of keratinizing squamous cell cervical carcinoma with giant cells. (biomedsearch.com)
  • A case of orbital metastasis of cervical keratinizing squamous cell carcinoma is presented. (biomedsearch.com)
  • Our previous study using proteomic profiling demonstrated significant up-regulation of Septin1, a conserved family of GTPase proteins, in oral squamous-cell carcinoma (OSCC)-derived cell lines. (biomedsearch.com)
  • In this study, the role of HPV in 72 patients with anal squamous cell carcinoma was investigated. (biomedsearch.com)
  • negative
  • All cell lines were negative for Mycoplasma infection. (aacrjournals.org)
  • Using EasySep™ technology, RoboSep™-S performs all steps necessary to magnetically label and separate virtually any cell type by positive or negative selection. (stemcell.com)
  • polymerase
  • By reverse transcriptase-polymerase chain reaction (RT-PCR), however, basal levels of CXCR-4 mRNA were also detected in all leukemic cell lines. (bloodjournal.org)
  • Depletion
  • The 2-step protocol was developed for processing 20 mL blood and based on red blood cell lysis followed by leukocyte depletion. (biomedcentral.com)
  • Implications
  • These differences could have major functional implications, particularly in relation to DC-T cell interactions, the so-called immunological synapse, and, therefore, need to be considered when interpreting data obtained from model DC systems. (biomedcentral.com)
  • Furthermore
  • Furthermore, these techniques can be employed under sterile conditions, allowing the enrichment of a small tumor cell population from peripheral blood, which may be grown in culture for functional investigations in order to elucidate their biology. (biomedcentral.com)
  • Furthermore, recent studies have shown that mTOR is also involved in cell death, so that a deregulation of this kinase could lead to the activation of antiapoptotic mechanisms ( 16 , 17 ). (aacrjournals.org)