Cells histidine. Medical search. Frequent questions

In addition to kinase activity, the intracellular domains typically have regions that bind to a secondary effector molecule or complex of molecules that further propagate signal transduction within the cell. (wikipedia.org)
Distinct from other classes of protein kinases, HKs are usually parts of a two-component signal transduction mechanisms in which HK transfers a phosphate group from ATP to a histidine residue within the kinase, and then to an aspartate residue on the receiver domain of a response regulator protein (or sometimes on the kinase itself). (wikipedia.org)
In terms of enzymology, a histidine kinase (EC 2.7.13.3, EnvZ, histidine protein kinase, protein histidine kinase, protein kinase (histidine), HK1, HP165, Sln1p) is an enzyme that catalyzes the chemical reaction ATP + protein L-histidine ⇌ {\displaystyle \rightleftharpoons } ADP + protein N-phospho-L-histidine. (wikipedia.org)
The mechanism for the reactions catalyzed by histidine kinase have not been completely elucidated, but current evidence suggests that the catalytic domain of one dimeric unit may rotate in such a way that the ATP binding pocket of that unit can come into contact with a particular histidine residue on the opposite unit and a nucleophilic addition results in a phosphorylated histidine. (wikipedia.org)
Histidine kinase 2 (Hik2) is a sensor histidine kinase found in all known cyanobacteria and as chloroplast sensor kinase in eukaryotic algae and plants. (biorxiv.org)
Histidine kinase 2 is a sensor of sodium ion concentration and redox potential, regulating transcription of genes for light-harvesting and reaction center proteins of photosynthesis in cyanobacteria and chloroplasts of algae and plants. (biorxiv.org)
Using radiolabeling, chemical crosslinking, chromatography and electron microscopy, we find that sodium ion concentration governs the oligomeric state of Histidine Kinase 2 and its phosphorylation by ATP. (biorxiv.org)
The objectives of the proposed Transnational Network are to bring together scientists from academia and industry working in relevant multidisciplinary fields to develop integrated strategies to overcome the permeability barrier of Gram-negative cell envelopes and further develop newly discovered histidine kinase inhibitors as anti-virulence therapies. (wur.nl)
BvgS isa histidine kinase sensor on the inner membrane. (kenyon.edu)

Our hypothesis is that this effect is of electrostatic origin and that the observed behavior is due to proton charge fluctuations of the histidines, promoting attractive electrostatic interactions between the positively charged proteins and the anionic surfaces, with concomitant counterion release. (lu.se)
Histidine kinases (HK) are multifunctional, and in non-animal kingdoms, typically transmembrane, proteins of the transferase class of enzymes that play a role in signal transduction across the cellular membrane. (wikipedia.org)
In marked contrast to Ser, Thr and Tyr phosphorylation, the analysis of phosphorylated Histidine using standard biochemical and mass spectrometric approaches is much more challenging, and special procedures and separation techniques are required for their preservation alongside classical Ser, Thr and Tyr phosphorylation on proteins isolated from human cells. (wikipedia.org)
Histidine-containing phosphotransfer proteins (HPts) take part in hormone signal transduction in higher plants. (rcsb.org)
HPts function in histidine-aspartate phosphorelays in which they mediate the signal from sensory kinases (usually membrane proteins) to RRs in the nucleus. (rcsb.org)
The chromatography medium provides very high binding capacity for histidine-tagged proteins and shows negligible leakage of Ni 2+ ions. (sigmaaldrich.com)
Ni Sepharose High Performance precharged with Ni2+ for high-performance puriﬁcation of histidine-tagged proteins. (sigmaaldrich.com)
To prevent the binding of host cell proteins with exposed histidines, it is essential to include imidazole at a low concentration in the sample and binding buffer ( Optimizing purification of histidine-tagged proteins ). (sigmaaldrich.com)
We are interested how extracellular matrix proteins instruct cells to differentiate, migrate, and proliferate, and how cells sense normal and pathologically altered extracellular matrix. (mcgill.ca)
This Special Issue, "Plant Cell Wall Proteins and Development", will cover a selection of recent research topics in the field of cell wall biology focused on cell wall proteins and their roles during development. (mdpi.com)
They mainly comprise polymers like polysaccharides and lignin in lignified secondary walls and a minute amount of cell wall proteins (CWPs). (mdpi.com)
Cell wall proteomics, as well as numerous genetic or biochemical studies, have revealed the high diversity of CWPs, among which proteins acting on polysaccharides, proteases, oxido-reductases, lipid-related proteins and structural proteins. (mdpi.com)
It is also possible to capture the entire population of proteins in cells that bind to metals such as copper or iron, the so-called metalloproteome," adds Dr. Boronowsky. (genengnews.com)
Six to eight histidines are typically used to increase specificity over intrinsic histidine in contaminating proteins. (genengnews.com)
GE Healthcare has announced the availability of its single-use spin column designed for purification of histidine-tagged proteins using a standard microcentrifuge. (outsourcing-pharma.com)
can phosphorylate substrate proteins on serine, threonine, tyrosines or histidine residues. (horizondiscovery.com)
Thus, the proteins that transport zinc likely facilitate cell signaling processes that contribute to glycemic control in peripheral tissues by modulating cytosolic zinc concentrations. (hindawi.com)
Cell surface biotinylation and immunofluorescence analyses with confocal microscopy indicated that rMATE1 mutant proteins were localized at plasma membranes. (aspetjournals.org)
Individual cells are supported by a broad array of proteins with a variety of functions. (elifesciences.org)

In polyhistidine tagging, 6 to 10 histidine residues are added recombinantly to either the protein's C- or N-terminus. (genengnews.com)
Previous studies using rat renal brush-border membrane vesicles indicated that cysteine and histidine residues played critical roles in H + /organic cation antiport activity. (aspetjournals.org)
In the present study, essential histidine and cysteine residues of MATE1 family were elucidated. (aspetjournals.org)
When 7 histidine and 12 cysteine residues of rat (r)MATE1 conserved among species were mutated, substitution of His-385, Cys-62, and Cys-126 led to a significant loss of tetraethylammonium (TEA) transport activity. (aspetjournals.org)
These results suggest that histidine and cysteine residues are required for MATE1 to function and that cysteine residues may serve as substrate-recognition sites. (aspetjournals.org)

More recently, the widespread existence of protein histidine phosphorylation distinct from that of two-component histidine kinases has been recognised in human cells. (wikipedia.org)
Two-component signal transduction systems (TCSs) consist of sensor histidine kinases and response regulators. (biorxiv.org)
We used loss-of-function mutants to study three Arabidopsis thaliana sensor histidine kinases, AHK2, AHK3, and CRE1/AHK4, known to be cytokinin receptors. (nih.gov)
Mitogen-activated protein kinases such as RAF kinases involved in the MAPK signaling pathways and cyclin dependent kinases (CDKs) involved in cell cycle regulation are prime examples of pathways implicated in cancers. (horizondiscovery.com)
We have previously identified a panel of inhibitors targeting bacterial histidine kinases in bacteria that inhibit targets involved in the regulation of virulence and stress response pathways in bacterial pathogens (Velikova et al. (wur.nl)
The activities of this Transnational Network are aimed at aligning international research activities and combining expertise to devise the most efficient strategy to further develop new anti-infective drugs targeted to histidine kinases. (wur.nl)

The transport of TEA via rMATE1 was inhibited by the sulfhydryl reagent p -chloromercuribenzenesulfonate (PCMBS) and the histidine residue modifier diethyl pyrocarbonate (DEPC) in a concentration-dependent manner. (aspetjournals.org)
All copper-transporting ATPases have a histidine residue in the large cytoplasmic loop adjacent to the ATP-binding domain. (medscape.com)
The histidine residue is the most common mutation site in Wilson disease, and this histidine residue is essential for the function of the Menkes ATPase, ATP7A. (medscape.com)

Blood Cells Mol Dis 2010(3): 246-65 [ PubMed abstract ]. (lu.se)

MW) chemicals interact with host cells and molecules and In our daily life, we are confronted with a plethora of natural influence biologic processes such as signal transduction. (cdc.gov)

The autophosphorylation H-box is contained in the N-terminal dimerization and histidine phosphotransfer (DHp) domain. (wikipedia.org)

Christine Jacobs-Wagner's studies of a bacterial species have changed how scientists think about cell shape and polarity. (the-scientist.com)
Over the past 10 years that success has played out in a handful of papers in Cell, Science, and other high-impact journals, where Jacobs-Wagner, now at Yale University, has showcased her award-winning work on bacterial physiology and development. (the-scientist.com)
Now there's a whole new field of people who are working on bacterial cell biology using the same sorts of approaches used to study eukaryotes. (the-scientist.com)

Precursor of histamine by action of histidine decarboxylase. (sigmaaldrich.com)
Imidazole derivatives are widely found in nature, such as histamine, histidine and benzimidazole, etc. (lookchem.com)

The histidine phosphorylation site is located at His-260. (wikipedia.org)

The test chemical did not induce chromosome aberrations in the mammalian cell line in the presence and absence of S9 metabolic activation system and hence it is not mutagenic in the chromosome aberration study performed. (europa.eu)
Test chemical did not induce mutation in mammalian cell line in the presence and absence of metabolic activation and hence it is not likely to classify as a gene mutant in vitro. (europa.eu)
Based upon an equal result in the tests of all oils from the Citrus category, the results of the available gene mutation tests in mammalian cells with a citrus oil can be read across to the other oils. (europa.eu)
For both orange oil and lemon oil, the gene mutation study in mammalian cells was negative. (europa.eu)
Expression of cloned envelope protein genes from the flavivirus tick-borne encephalitis virus in mammalian cells and random mutagenesis by PCR. (microbiologyresearch.org)
The protein diversity of mammalian cells is determined by arrays of isoforms from genes. (elifesciences.org)
Accurate Long-read transcriptome sequencing at single-cell level is required to decipher the spectrum of protein expressions in mammalian organisms. (elifesciences.org)
In conclusion, LOOPseq single-cell technology may hold promise to provide a new level of precision analysis on the mammalian transcriptome. (elifesciences.org)
Mammalian organisms are composed of numerous cells with multiple different roles. (elifesciences.org)

Here we probe these fundamental questions using peroxisomal compartmentalization of the last steps of lysine and histidine biosynthesis in the fission yeast Schizosaccharomyces japonicus . (biorxiv.org)

Activation of endothelial cells via antibody-enhanced dengue virus infection of peripheral blood monocytes. (microbiologyresearch.org)

The sensor component is a protein that becomes covalently modified by a phosphate group on a histidine side chain. (biorxiv.org)

Through Uniform Manifold Approximation and Projection (UMAP) analysis, we identified a panel of mutation mRNA isoforms highly specific to HCC cells. (elifesciences.org)
The combination of gene expressions, fusion gene transcripts, and mutation gene expressions significantly improved the classification of liver cancer cells versus benign hepatocytes. (elifesciences.org)

To understand the role of each cell in an organism, broad spectrum mRNA isoform and mutational gene expression analyses at the single-cell level is necessary. (elifesciences.org)

Histatin 5 is a histidine-rich, intrinsically disordered, multifunctional saliva protein known to act as a first line of defense against oral candidiasis caused by Candida albicans. (lu.se)
Multifunctional receptor molecules such as HKs and RTKs typically have portions on the outside of the cell (extracellular domain) that bind to hormone- or growth factor-like molecules, portions that span the cell membrane (transmembrane domain), and portions within the cell (intracellular domain) that contain the enzymatic activity. (wikipedia.org)

Methodologically, the lab employs a wide spectrum of techniques ranging from in vitro biochemical and biophysical assays, to cell biology methods with primary cells and transformed cell lines, to light and electron microscopic techniques, to in vivo studies with genetically modified mouse models. (mcgill.ca)

B. bronchiseptica" infects healthy ciliated epithelial cells whereas most respiratory pathogens cannot. (kenyon.edu)
The Menkes protein is also in retinal pigment epithelial cells and the neurosensory retina. (medscape.com)

Am J Physiol Cell Physiol 323: C354-C366. (mcgill.ca)
Am J Physiol Cell Physiol 323: C536-C549. (mcgill.ca)

Role of histidine for charge regulation of unstructured peptides at interfaces and in bulk. (lu.se)

Carnosine (β-alanine-histidine) is an endogenous histidyl dipeptide, present in high concentration in the skeletal muscle (1-20 mM), heart (0.1-1 mM), and brain (1-2 mM). (frontiersin.org)

To investigate the interrelationship between these two systems further, we have studied the orexinergic inputs to the TM nucleus using intracellular recordings and single-cell PCRs from TM neurons as well as immunocytochemical methods. (jneurosci.org)

Here we are investigating the role of the histidines in more detail by defining a library of variants of the peptide, where. (lu.se)
Here we are investigating the role of the histidines in more detail by defining a library of variants of the peptide, where the former have been replaced by the pH-insensitive amino acid glutamine. (lu.se)
By using experimental techniques such as circular dichroism, small angle X-ray scattering, quartz crystal microbalance with dissipation monitoring, and neutron reflectometry, it was determined that changing the number of histidines in the peptide sequence did not affect the structure of the peptide dissolved in solution. (lu.se)
However, it was shown to affect the penetration depth of the peptide into the bilayer, where all variants except the one with zero histidines were found below the bilayer. (lu.se)
A decrease in the number of histidine from the original seven to zero decreases the ability of the peptide to penetrate the bilayer, and the peptide is then also found residing within the bilayer. (lu.se)
We hypothesize that this is due to the ability of the histidines to charge titrate, which charges up the peptide, and enables it to penetrate and translocate through the lipid bilayer. (lu.se)
Using the mutational analysis of recombinant subviral particles of tick-borne encephalitis virus, we provide direct experimental evidence that the initiation of fusion is crucially dependent on the protonation of one of the conserved histidines (His323) at the interface between domains I and III of E, leading to the dissolution of domain interactions and to the exposure of the fusion peptide. (rupress.org)

In a single-cell RT-PCR study using primers for the two orexin receptors, we found that most tuberomammillary neurons express both receptors and that the expression of the orexin-2 receptor is stronger than that of the orexin-1 receptor. (jneurosci.org)
It is now clear that the ability of T cells to promote an effective immune response depends on a large repertoire of unique T-cell receptors (TCRs) generated and selected in the thymus. (frontiersin.org)

Here, we report on a novel human endothelial cell-based recycling assay (HERA) that can be used for such pre-clinical screening. (nature.com)
The strategy of incorporating LoopSeq long-read technology with single-cell sequencing starts with utilizing the output of 10x Genomics' 3' single-cell assay. (elifesciences.org)

High (millimolar) concentrations of the histidine containing dipeptide - carnosine (β-alanine-L-histidine) are present in the skeletal muscle. (frontiersin.org)
Abnormal copper transport in other cells leads to paradoxical copper accumulation in duodenal cells, kidney, pancreas, skeletal muscle, and placenta. (medscape.com)

In an in vitro chromosomal aberration test using a Chinese hamster fibroblast cell line (CHL) (OECD Guideline 473), lime oil did not significantly induce chromosomal aberrations in CHL cells in vitro at three different concentrations in the absence of metabolic activation and was therefore considered not clastogenic. (europa.eu)
At the cellular level, total zinc concentrations of human cells are 200-300 μ M [ 20 ]. (hindawi.com)

Relationship between hematogenous tumor cell dissemination and cellular immunity in DCIS patients. (nature.com)
The identification of lymphocytes as the main cell type responsible for both cellular and humoral immunity started in the early 1950s with the emergence of cell culture techniques. (frontiersin.org)

Her pioneering studies on the molecular mechanisms underlying cell shape and cell polarity in Caulobacter crescentus , says Errington, "have helped change the way people think about bacteria. (the-scientist.com)
Cytotoxin production by other bacteria (ie, Shigella dysenteriae, Vibrio parahaemolyticus, Clostridium difficile, enterohemorrhagic E coli ) results in mucosal cell destruction that leads to bloody stools with inflammatory cells. (medscape.com)

Most studies have focused on the identification of memory T cells that recognize drugs/chemicals and the insights obtained have led to the development of allergy diagnostic tests ( 7 - 21 ). (frontiersin.org)

The plates were observed for a dose dependent increase in the number of Histidine- independent (his+) colonies. (europa.eu)

Li C, Sato K, Monari S, Salard I, Sola M, Banfield MJ, Dennison C. Metal-Binding Loop Length Is a Determinant of the pK(a) of a Histidine Ligand at a Type 1 Copper Site . (ncl.ac.uk)
Schlief et al found that copper is protective and copper chelation exacerbates NMDA-mediated excitotoxic cell death in hippocampal neurons. (medscape.com)
Under normal circumstances, ATP7A transports copper into the secretory pathway of the cell for incorporation into the cuproenzymes and excretion from the cell. (medscape.com)
As the copper is concentrated into vesicles for excretion from the cell, the cytosolic copper concentration decreases and ATP7A returns to the trans-Golgi network. (medscape.com)
In Menkes disease, transport of dietary copper from intestinal cells is impaired, leading to the low serum copper levels. (medscape.com)

L-Histidine is an essential amino acid. (sigmaaldrich.com)
These factors are short peptides with molecular weights of 1,000 to 7,000 that contain the amino acid histidine. (usda.gov)
DESCRIPTION: Histidine is a semi-essential amino acid. (drstandley.com)
Nine of them are called "essential" amino acids: These organic molecules obtained from protein-containing foods are crucial to countless processes in our bodies, including giving cells their structure, forming organs and muscles, repairing tissue, producing energy, and more. (mindbodygreen.com)
The essential amino acid histidine is needed for the growth and repair of tissue, particularly for the maintenance of myelin sheaths-sleeves of fatty tissue that protect nerve cells, ensuring that they're able to send and receive messages. (mindbodygreen.com)

Histidine dipeptides are key regulators of excitat. (fapesp.br)

Thus, the two substrates of this enzyme are ATP and protein L-histidine, whereas its two products are ADP and protein N-phospho-L-histidine. (wikipedia.org)
Working with experts in protein chemistry, genetics, beta-lactamase biochemistry, cell wall synthesis, and medical microbiology - in Belgium, France, the United States, and Sweden - Jacobs-Wagner discovered a regulatory protein that can sense the peptidoglycans that accumulate when antibiotics disrupt cell wall synthesis, and then activate transcription of beta-lactamase, the enzyme that disarms the drugs. (the-scientist.com)
People knew there was a connection between enzyme induction and cell wall breakdown," he says. (the-scientist.com)
Succinate:quinone oxidoreductase (SQR) is both a Krebs´ citric acid cycle enzyme and a component (Complex II) of the respiratory chain in aerobic cells. (lu.se)

The entry of enveloped viruses into host cells involves a fusion step between the viral and a cellular membrane. (rupress.org)
Given the low plasma concentration of zinc and its importance in cellular signaling, it is essential that the availability and distribution of "free" zinc (free zinc is used to differentiate zinc involved in cell signaling from zinc that tightly bound to protein and therefore thermodynamically unavailable) are tightly controlled [ 6 ]. (hindawi.com)

American Journal of Respiratory Cell and Molecular Biology. (lu.se)

Leaves of ahk2 ahk3 mutants formed fewer cells, had reduced chlorophyll content, and lacked the cytokinin-dependent inhibition of dark-induced chlorophyll loss, indicating a prominent role of AHK2 and, particularly, AHK3 in the control of leaf development. (nih.gov)
Fewer or more histidines are also possible. (genengnews.com)

J Cell Biol (2008) 183 (2): 353-361. (rupress.org)
J Cell Biol (2004) 165 (6): 869-880. (rupress.org)

Specifically, FHA binds directly to the ciliary membrane host-receptor glycospingolipids, allowing "B. bronchiseptica" to attach to the host's cells. (kenyon.edu)
In the Fp subunit of SQR, and the closely related membrane-associated fumarate reductase of anaerobic organisms, FAD is covalently attached via an 8α-N(3)-histidyl linkage to a histidine (His) in a conserved sequence in the N-terminal part of the polypeptide. (lu.se)

Blue cells = expressed in wild-type. (jax.org)
On one hand, drug-specific T cells provide the necessary help for mounting an effective B-cell response observed in immediate-type hypersensitivity reactions. (frontiersin.org)

ATP7A increases in CA2 hippocampal pyramidal and cerebellar Purkinje neurons but decreases in other cell populations postnatally. (medscape.com)

Histidine to repair damaged tissue and improve blood cell production. (wishtv.com)

However, the E. coli cells did not show increased succinate dehydrogenase activity nor did the operon complement a sdhCDAB defective E. coli mutant [7]. (lu.se)
The B. subtilis Fp polypeptide was found to lack covalently bound FAD when produced in E. coli although E. coli Fp in the same cell was flavinylated [8]. (lu.se)

for example, they provide structural stability to cells and tissues, give motility to individual cells, carry messages within and between cells, and regulate gene expression and metabolism. (biologists.com)

This discrepancy is explained by thymic selection where the fate of T-cell precursors is dependent on the recognition of self-peptides (self-p) presented by MHC molecules on thymic stromal cells ( 28 ). (frontiersin.org)

The results showed evolutionary patterns of single molecule mutational gene expression from benign hepatocytes to liver cancer cells. (elifesciences.org)

Conserved histidines located outside this critical interface were found to be completely dispensable for triggering fusion. (rupress.org)
On the other hand, a large T-cell repertoire found in multiple donors would underline the potential of a drug/chemical to be recognized by many donors. (frontiersin.org)
HER2 is found in large amounts on the surface of some cancer cells where it stimulates their growth. (who.int)

Pretreatment of murine myoblast (C2C12) cells with octyl-D-carnosine or carnosine enhanced HIF-1α protein expression, VEGF mRNA levels and VEGF release under hypoxic conditions. (frontiersin.org)
However, little progress has been made in developing a technology to analyze mutated mRNA expressions at the single-cell level. (elifesciences.org)

Histidine is widely present at the active sites of enzymes. (sigmaaldrich.com)

Each swarmer cell will eventually differentiate into a stalk cell and begin the cycle again. (the-scientist.com)

Allergic reactions to drugs and chemicals are mediated by an adaptive immune response involving specific T cells. (frontiersin.org)
T cells are central to allergic reactions. (frontiersin.org)
On the other hand, T cells constitute the main pathogenic effector cells in delayed hypersensitivity reactions ( 4 - 6 ). (frontiersin.org)

The aim of this review is to address the existence and the role of a naive T-cell repertoire in drug and chemical allergy. (frontiersin.org)

When [BT-ON013 trade name] binds to HER2 it stops the growth of such cells and causes them to die. (who.int)

Drug and Chemical Allergy: A Role for a Specific Naive T-Cell Repertoire? (frontiersin.org)
POTENTIAL BENEFITS: Helps in the role of allergies, the production of red (thereby preventing anemia) and white blood cells. (drstandley.com)

Organisms4

Chemicals and Drugs49

Analytical, Diagnostic and Therapeutic Techniques and Equipment14

Phenomena and Processes27

Disciplines and Occupations1

Information Science3

Kinase9

Proteins19

Residues5

Kinases6

Residue3

Abstract1

Signal transduction1

Phosphotransfer1

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Histamine2

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Mammalian9

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Endothelial cells1

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Physiol2

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Peptide7

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