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  • progenitors
  • Highly purified in vivo transplantable mouse stem cells are maintained in AFT024 cultures at input levels, whereas other primitive progenitors are expanded. (pnas.org)
  • G-CSF), some myelosuppressive drugs used in cancer treatment, and compounds that disrupt the interaction between hematopoietic and BM stromal cells can rapidly mobilize large numbers of stem and progenitors into the circulation. (stemcell.com)
  • therapies
  • The discovery has implications for developing stem cell-based therapies for diseases like leukemia and congenital blood disorders. (redorbit.com)
  • Stem cells hold great promise for regenerative medicine and cell-based therapies ( 1 ). (pnas.org)
  • The second edition is fully revised and includes new chapters on microbiome, metabolism, kinase targets, micro-RNA and mRNA regulatory mechanisms, signaling pathways in GVHD, innate lymphoid system development, recovery and function in GVHD, genetically engineered T-cell therapies, immune system engagers for GVHD and graft-versus-tumor, and hematopoietic cell transplant for tolerance induction in solid organ grafts. (elsevier.com)
  • HSPCs are also being investigated in cell-based therapies for non-hematopoietic disorders. (stemcell.com)
  • Methods
  • In 'Nature Methods' they describe how their software predicts the future cell type based on microscopy images. (innovations-report.com)
  • Hematopoietic stem cells under the microscope: New methods are helping the Helmholtz scientists to predict how they will develop. (innovations-report.com)
  • Compared to conventional methods, such as fluorescent antibodies against certain surface proteins, we know how the cells will decide three cell generations earlier," reports ICB scientist Dr. Felix Buggenthin, joint first author of the study together with Dr. Florian Büttner. (innovations-report.com)
  • plasticity
  • This view has been challenged by recent observations that suggest a high degree of plasticity or transdifferentiation for stem cells ( 3 - 5 ). (pnas.org)
  • vivo
  • They used a specific dose (5uM) of a pharmacologic inhibitor of Cdc42, CASIN, to reduce the protein's activity in the cells - processing them for 16 hours ex vivo in laboratory cultures. (redorbit.com)
  • We suggest that the AFT024 cell line represents a component of an in vivo stem cell niche. (pnas.org)
  • These were tested for their ability to support primitive hematopoietic stem cells in ex vivo cultures. (pnas.org)
  • Human hematopoietic stem/progenitor cells modified by zinc-finger nucleases targeted to CCR5 control HIV-1 in vivo," Nature Biotechnology , vol. 28, no. 8, pp. 839-847, 2010. (hindawi.com)
  • HSC population is then the ideal target for the correction of hematopoietic genetic diseases and also for the knockout of the responsible genes to in vitro and in vivo model those hematopoietic diseases. (intechopen.com)
  • Advances
  • An opportunity for scientists, clinicians, educators and industry professionals to share new data, learn from peers, and discover global advances within the stem cell field. (constantcontact.com)
  • cytokines
  • An RNA expression survey for known stem cell regulatory cytokines, both positive and negative, did not reveal significant differences among supportive and nonsupportive cell lines ( 8 ). (pnas.org)
  • survival
  • who found that signaling induced by a microbe-derived agonist of NOD2 promoted survival of intestinal stem cells and improved their resistance to oxidative stress-induced cell death. (sciencemag.org)
  • In one type of assay, lethally irradiated recipient mice are coinjected with congeneic donor-derived "test" cells along with syngeneic (host-type) "competitor" cells to provide shortterm radioprotection, ensure survival, and provide a selective pressure to identify stem cells with high competitive repopulating potential. (stemcell.com)
  • After sublethal irradiation these animals can be transplanted with donor "test" cells from wild-type mice without the need for co-transplanted radioprotective cells to promote survival. (stemcell.com)
  • sickle-cell di
  • Other conditions treated with stem cell transplants include sickle-cell disease , myelodysplastic syndrome , neuroblastoma , lymphoma , Ewing's sarcoma , desmoplastic small round cell tumor , chronic granulomatous disease , Hodgkin's disease and Wiskott-Aldrich syndrome . (wikipedia.org)
  • vitro
  • In vitro , various model systems have been employed to mimic thymic function supportive of T-cell development. (sigmaaldrich.com)
  • phenotype
  • We reasoned that molecules responsible for the stem cell supportive phenotype would be preferentially expressed in AFT024 compared with nonsupporting lines derived from the same tissue source. (pnas.org)
  • niche
  • An important feature of stem cells is their maintenance in their respective hypoxic niche. (springer.com)
  • Simsek T, Kocabas F, Zheng J, DeBerardinis RJ, Mahmoud AI, Olson EN et al (2010) The distinct metabolic profile of hematopoietic stem cells reflects their location in a hypoxic niche. (springer.com)
  • The data have been assembled into a biological process oriented database, and represent a molecular profile of a candidate stem cell niche. (pnas.org)
  • mouse cells
  • The findings are early and involve laboratory manipulation of mouse cells, so it remains to be seen what direct application they may have for humans. (redorbit.com)