• Looking at the distribution of DNA in the nucleus, they discovered that much of the change in the viscoelastic nature of the nuclei is to do with the aggregation of chromatin, multi-component structures made of DNA and proteins. (scitechdaily.com)
  • In addition, our experiments show that heat-treating donor nuclei destabilizes higher-order features of chromatin (but leaves intact its nucleosomal organization) and results in a high proportion of reconstructed embryos developing to the blastocyst stage and beyond. (cnrs.fr)
  • Megaloblasts are large nucleated red blood cell (RBC) precursors with noncondensed chromatin due to impaired DNA synthesis. (msdmanuals.com)
  • Eukaryotic cells usually have a single nucleus, but a few cell types, such as mammalian red blood cells, have no nuclei, and a few others including osteoclasts have many. (wikipedia.org)
  • Lastly, we compare this single-cell data to that generated using three single-nucleus RNA sequencing workflows. (biomedcentral.com)
  • Finally, cell type composition differences are observed between single-cell and single-nucleus RNA sequencing libraries. (biomedcentral.com)
  • In particular, we note an underrepresentation of T, B, and NK lymphocytes in the single-nucleus libraries. (biomedcentral.com)
  • The genes within these chromosomes are structured in such a way to promote cell function. (wikipedia.org)
  • Although the interior of the nucleus does not contain any membrane-bound subcompartments, a number of nuclear bodies exist, made up of unique proteins, RNA molecules, and particular parts of the chromosomes. (wikipedia.org)
  • Inside the nucleus are forty-six threadlike structures known as chromosomes, and each one of these structures contains thousands of genes. (innerbody.com)
  • Nucleus with chromosomes, DNA molecule (double helix), telomere and gene (length of DNA that codes for a specific protein). (istockphoto.com)
  • Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). (harvard.edu)
  • Each of our cells houses all its chromosomes inside the nucleus, as well as many specialized proteins that regulate how our genes switch on or off. (alleninstitute.org)
  • Genomic data describes the makeup of the chromosomes inside a cell's nucleus and is typically represented as one- or two-dimensional data. (cmu.edu)
  • Imaging data and chromosome structure models, which show how chromosomes are folded and where they are located inside the nucleus, are three-dimensional in nature. (cmu.edu)
  • 14 Embedded within the inner membrane, various proteins bind the intermediate filaments that give the nucleus its structure. (wikipedia.org)
  • This size selectively allows the passage of small water-soluble molecules while preventing larger molecules, such as nucleic acids and larger proteins, from inappropriately entering or exiting the nucleus. (wikipedia.org)
  • Histones are proteins that keep DNA strands curled up and packed into the cell nucleus. (sciencedaily.com)
  • The pores are filled with special proteins, called nucleoporins, which select what can move in or out of the nucleus. (rug.nl)
  • Biologists at the Allen Institute have further engineered these stem cells to tag certain proteins or cellular structures with glowing fluorescent labels. (alleninstitute.org)
  • However, in an investigation of BRAF localisation using fluorescence microscopy combined with subcellular fractionation of Green Fluorescent Protein (GFP)-tagged proteins expressed in NIH3T3 cells, surprisingly, we detected N-terminally truncated BRAF (ΔBRAF) in both nuclear and cytoplasmic compartments. (bath.ac.uk)
  • Even without stimulation, Forkhead is constantly moving into the nucleus, but is transported out again by export proteins. (broadinstitute.org)
  • The nucleus is also responsible for the production of proteins, which are essential for a wide range of functions in the body. (the-one.world)
  • These cells, in an image captured by scientist Chris Frick , Ph.D., are engineered with a label that marks the nuclear envelope, the thin membrane that surrounds the cell's nucleus. (alleninstitute.org)
  • Researchers in the School of Computer Science's Computational Biology Department have developed a tool that aims to help scientists gain a fuller and deeper understanding of the structure and function of a cell's nucleus. (cmu.edu)
  • IVDs are composed of two distinct components: The inner gel-like core nucleus pulposus (NP) and the outer firm annulus fibrosus (AF). (spandidos-publications.com)
  • The intervertebral disc (IVD) consists of a central gelatinous nucleus pulposus (NP) encircled by an elastic, ligamentous annulus fibrosus (AF) and is flanked superiorly and inferiorly by cartilaginous endplates. (biomedcentral.com)
  • Delivering Growth Factors through a Polymeric Scaffold to Cell Cultures Containing both Nucleus Pulposus and Annulus Fibrosus. (bvsalud.org)
  • and collagen type II (COL2A1) gene expressions were determined with quantitative real-time polymerase chain reaction (qRT- PCR ) analysis to show the effects of IGF-1 /BMP-2 administration on annulus fibrosus cell (AFC)/ nucleus pulposus cell (NPC) cultures . (bvsalud.org)
  • In a way, Prof Takemura's hypothesis has its roots in 2001 when, along with PJ Bell, he made the revolutionary proposal that large DNA viruses, like the poxvirus, had something to do with the rise of the eukaryotic cell nucleus. (sciencedaily.com)
  • The 2001 hypothesis by Prof Takemura and PJ Bell is based on striking similarities between the eukaryotic cell nucleus and poxviruses: in particular, the property of keeping the genome separate in a compartment. (sciencedaily.com)
  • Bell proposed a viral origin for the eukaryotic nucleus back in 2001 and refreshed the theory in September. (uncommondescent.com)
  • It arises from conflict between the entraining cycle of light and darkness and the temporal program of the clock ( Czeisler and Dijk, 1995 ) within the hypothalamic suprachiasmatic nuclei (SCN) ( Reppert and Weaver, 2001 ). (jneurosci.org)
  • 5. In 2001, France and Germany requested the United Nations General Assembly to develop international conventions on human reproductive cloning, therapeutic cloning and research on stem cells. (who.int)
  • The nuclear pore complex is one of the biggest protein structures in the cell,' explains Patrick Onck, professor of Micromechanics at the University of Groningen. (rug.nl)
  • The movement of large molecules through the nuclear envelope and into the cell nucleus is regulated by large protein structures called nuclear pore complexes. (phys.org)
  • The term nucleus is also used in zoology for a few other structures, such as the digestive organs of salpa. (sacklunch.net)
  • Some of these parts, called organelles, are specialized structures that perform certain tasks within the cell. (medlineplus.gov)
  • is made up of a jelly-like fluid (called the cytosol) and other structures that surround the nucleus. (medlineplus.gov)
  • Structures that are part of or contained in the CELL NUCLEUS. (bvsalud.org)
  • Pores in this membrane allow communication between the nucleus and the rest of the cell. (rug.nl)
  • Together, both allow for a proper communication between the nucleus and cytoplasm, which is essential for the cell functioning. (cosmeticsbusiness.com)
  • Here we report on the successful reprogramming of nuclei from somatic cells rendered nonviable by heat treatment. (cnrs.fr)
  • Somatic-cell nuclear transfer, the technique by which Dolly was created, was first used 40 years ago in research with tadpoles and frogs. (who.int)
  • The nucleus of an adult somatic cell (such as a skin cell) is removed and transferred to an enucleated egg, which is then stimulated with electric current or chemicals to activate cell division. (who.int)
  • if it implants and the pregnancy goes to term, the resulting individual will carry the same nuclear genetic material as the donor of the adult somatic cell. (who.int)
  • Scientists were initially interested in somatic-cell nuclear transfer as a means of determining whether genes remain functional even after most of them have been switched off as the cells in a developing organism assume their specialized functions as blood cells, muscle cells, and so forth. (who.int)
  • Cellular reprogramming is a new and rapidly emerging field in which somatic cells can be turned into pluripotent stem cells or other somatic cell types simply by the expression of specific combinations of genes. (lu.se)
  • article{4b5db5d0-c1b6-463c-9ca2-f61b01429744, abstract = {{Cellular reprogramming is a new and rapidly emerging field in which somatic cells can be turned into pluripotent stem cells or other somatic cell types simply by the expression of specific combinations of genes. (lu.se)
  • Ecker shares ,"We think it's pretty striking that we can tease apart a brain into individual cells, sequence their methylomes, and identify many new cell types along with their gene regulatory elements, the genetic switches that make these neurons distinct from each other. (epigenie.com)
  • The resulting cells are nonproliferating and present an alternative to induced pluripotent stem cells for obtaining patient- and disease-specific neurons to be used for disease modeling and for development of cell therapy. (lu.se)
  • Using a transgenic mouse model to specifically direct expression of reprogramming genes to parenchymal astrocytes residing in the striatum, we also show that endogenous mouse astrocytes can be directly converted into neural nuclei (NeuN)-expressing neurons in situ. (lu.se)
  • However, as the result of recent advancements in microscopic imaging technology and computational image processing techniques [ 1 ], there has been significant growth of research towards translational computational systems that can detect, analyze, classify, and quantify cell nuclei from microscopic images. (hindawi.com)
  • To analyse the time-series 3D microscopic images of developing embryos with fluorescently labelled nuclei, these studies used image segmentation. (nature.com)
  • The genetic material delivered by mRNA vaccines never enters the nucleus of your cells, which is where your DNA is kept, so the vaccine does not alter your DNA. (cdc.gov)
  • Here, we compare gene expression and cellular composition of single-cell suspensions prepared from adult mouse kidney using two tissue dissociation protocols. (biomedcentral.com)
  • Upon phosphorylation, R-SMADs recruit the co-SMAD SMAD-4 to form protein complexes that translocate into the nucleus and exhibit transcriptional activity. (springer.com)
  • The protein labeled in these cells, known as lamin B1, is a key part of the nuclear envelope. (alleninstitute.org)
  • In addition, nuclear factor κB (NF‑κB), c‑Jun N‑terminal kinase (JNK), extracellular signal‑regulated kinases and p38 mitogen‑activated protein kinase inhibitors and TNF‑α were used to determine the molecular mechanism of autophagy during the inflammatory conditions, and only the NF‑κB and JNK inhibitor were found to enhance the autophagy of rat NP cells. (spandidos-publications.com)
  • This 96-well plate has images of cytoplasm to nucleus translocation of the Forkhead (FKHR-EGFP) fusion protein in stably transfected human osteosarcoma cells, U2OS. (broadinstitute.org)
  • From the intricate mechanisms of DNA to the orchestration of protein synthesis, the animal cell with a nucleus is a testament to the remarkable achievements and progress of life. (the-one.world)
  • Immortalized cells were clonally expanded and characterized based on collagen type I, collagen type II, α1 (COL2A1), and SRY-box 9 (SOX9) protein expression profiles, as well as on expression of a subset of established in vivo NP cell lineage markers. (biomedcentral.com)
  • Phospholipid content was determined by extraction of cells with chloroform and methanol and protein and DNA were measured. (cdc.gov)
  • Both messenger RNA (mRNA) and protein subunit COVID-19 vaccines work by delivering instructions (genetic material) to your cells to start building protection against the virus that causes COVID-19. (cdc.gov)
  • An exchange of genetic material that occurred when ancient giant viruses infected ancient eukaryotic cells could have caused the nucleus of the eukaryotic cell -- its defining feature -- to form. (sciencedaily.com)
  • The most widely accepted theories that have emerged state that the eukaryotic cell is the evolutionary product of the intracellular evolution of proto-eukaryotic cells, which were the first complex cells, and symbiotic relationships between proto-eukaryotic cells and other unicellular and simpler organisms such as bacteria and archaea. (sciencedaily.com)
  • Prof Takemura further explains the reasons for his inquiry into the nucleus of the eukaryotic cell as such: "Although the structure, function, and various biological functions of the cell nucleus have been intensively investigated, the evolutionary origin of the cell nucleus, a milestone of eukaryotic evolution, remains unclear. (sciencedaily.com)
  • The eukaryotic cell is neatly compartmentalized into membrane-bound organelles that perform various functions. (sciencedaily.com)
  • In the recent decades, with exponential advancement in the fields of genomics, molecular biology, and virology, several scientists on this quest have taken to looking into the evolutionary twists and turns that have resulted in eukaryotic cells, the type of cell that makes up most life forms today. (sciencedaily.com)
  • An adherent vessel preserves cell biology, but a cell suspension culture yields scale. (corning.com)
  • S2 Genomics is developing integrated sample preparation systems for processing tissues into genomic samples for single-cell genomics and cell biology studies. (bucher.ch)
  • Our cell lines yield novel insights into NP biology and provide promising new tools for studies of IVD development, cell function and disease. (biomedcentral.com)
  • The nucleus maintains the integrity of genes and controls the activities of the cell by regulating gene expression. (wikipedia.org)
  • Independent phylogenetic analyses suggested that genes had been transferred between these viruses and eukaryotic cells as they interacted at various points down the evolutionary road, in a process called "lateral gene transfer. (sciencedaily.com)
  • Further, unlike other viruses, it does not construct its own enclosed "viral factory" in the cytoplasm of the cell within which to replicate its DNA and contains none of the genes required to carry out the replication process. (sciencedaily.com)
  • Its ability to perform cold dissociation minimizes the expression of stress-related genes in cells and helps preserve RNA quality in nuclei. (bucher.ch)
  • The recent identification of mammalian circadian clock genes now makes it possible to examine time zone adjustments from the perspective of molecular events within the suprachiasmatic nucleus (SCN), the principal circadian oscillator. (jneurosci.org)
  • The fact that the DNA of a fully differentiated (adult) cell could be stimulated to revert to a condition comparable to that of a newly fertilized egg and to repeat the process of embryonic development demonstrates that all the genes in differentiated cells retain their functional capacity, although only a few are active. (who.int)
  • Accurate results of cell nuclei segmentation are often adapted to a variety of applications such as the detection of cancerous cell nuclei and the observation of overlapping cellular events occurring during wound healing process in the human body. (hindawi.com)
  • This triggered an increase in paracellular permeability via cellular redistribution of claudin 3 (CLDN3) from TJs into cell nuclei followed by disruption of epithelial integrity and formation of epithelial lesions. (springer.com)
  • We can see where cellular components are in the nucleus and how they interact with what's around them spatially. (cmu.edu)
  • This image shows an osteosarcoma cell with DNA in blue, energy factories (mitochondria) in yellow, and actin filaments, part of the cellular skeleton, in purple. (nih.gov)
  • Single-cell RNA sequencing has been widely adopted to estimate the cellular composition of heterogeneous tissues and obtain transcriptional profiles of individual cells. (biomedcentral.com)
  • Relatively little is known about cellular subpopulations in the mature nucleus pulposus (NP). (biomedcentral.com)
  • Detailed understanding of the ontogenetic, cellular and molecular characteristics of functional intervertebral disc (IVD) cell populations is pivotal to the successful development of cell replacement therapies and IVD regeneration. (biomedcentral.com)
  • Here we report the generation of clonal NP cell lines from nondegenerate human IVD tissue and present a detailed characterization of NP cellular subpopulations. (biomedcentral.com)
  • Successful development of cell replacement therapies and IVD regeneration is crucially dependent on an in-depth understanding of cellular and molecular characteristics of the functional IVD. (biomedcentral.com)
  • They calculated the CH methylation level for each neuron in non-overlapping 100-kb bins and clustered the cells according to their CH methylation profiles, where they annotated the clusters by known cell-type markers. (epigenie.com)
  • 5 . Luthman J, Hoebeek FE, Maex R, Davey N, Adams R, De Zeeuw CI, Steuber V (2011) STD-dependent and independent encoding of input irregularity as spike rate in a computational model of a cerebellar nucleus neuron. (yale.edu)
  • A cell nuclei segmentation algorithm incorporating unsupervised color clustering, morphological operations, and local thresholding has been proposed to distinguish the cancerous and noncancerous areas in histologically stained images and then segment the clustered cell nuclei [ 3 ]. (hindawi.com)
  • To quantify the nuclear region of every cell from a time-series 3D fluorescence microscopic image of living cells, we developed QCANet, a convolutional neural network-based segmentation algorithm for 3D fluorescence bioimages. (nature.com)
  • These large molecules must be actively transported into the nucleus instead. (wikipedia.org)
  • In the cell, large molecules that must be transported into or out of the nucleus can only do so when they are attached to such a chaperone. (rug.nl)
  • Entropic-based thresholding methods for cell nuclei segmentation are proposed by Wang and Gudla et al. (hindawi.com)
  • The latter name is given to it from its property of absorbing staining reagents much more readily than the rest of the cell. (sacklunch.net)
  • S2 Genomics provides a selection of pre-set protocols and pre-formulated reagents for cell isolations for an expanding set of mouse, rat, and human tissues, including tumors. (bucher.ch)
  • The Singulator 100TM System comes with a unique Single-Shot MechanismTM for convenient cell-isolation reagent loading and a Reagent Chiller that stores reagents for up to 100 nuclei runs. (bucher.ch)
  • Stevenage Bioscience Catalyst's new Spark Building opened its doors in January to accommodate the rapid growth of scale-up businesses within Hertfordshire's world-class cell and gene cluster. (onenucleus.com)
  • The Spark Building was built to accommodate the companies scaling up to create a new wave of advanced cell and gene therapy treatments with the potential to cure cancer and other life-threatening diseases. (onenucleus.com)
  • SBC is a world-leading science park of global significance, at the centre of the largest cluster of cell and gene therapy companies in Europe. (onenucleus.com)
  • Since its opening in 2012, occupiers of the SBC campus have raised over £1.6bn of investment, around 65% of which has been invested into cell and gene therapy companies. (onenucleus.com)
  • Dr Sally Ann Forsyth, Chief Executive Officer at SBC, commented: "The Spark Building ensures that we maintain our position as Europe's leading hub for cell and gene therapy and underlines the importance to the UK of capabilities here in Stevenage. (onenucleus.com)
  • Paul Witcombe, Head of Enterprise and Innovation, Hertfordshire LEP, said: "Our £1.2m investment has helped provide the immediate accommodation needed to support the continued expansion of the burgeoning cell and gene therapy cluster in Stevenage, to further strengthen Hertfordshire's vibrant life sciences ecosystem and ultimately provide high quality jobs for our local people. (onenucleus.com)
  • I am pleased that this important work is underway providing jobs for local people and strengthening Hertfordshire as a leading hub for cell and gene therapy. (onenucleus.com)
  • The Spark Building, co-located alongside the SBC Incubator and Accelerator Buildings, and the Cell and Gene Therapy Catapult Manufacturing Centre, has supported the scale up of outstanding cell and gene therapy companies such as Achilles Therapeutics, Autolus, Freeline and TCR 2 Therapeutics. (onenucleus.com)
  • In parallel to the Spark Building, and supported by a further £2.92m Local Growth Fund investment from Hertfordshire LEP, the Cell and Gene Therapy Catapult Manufacturing Centre is also undergoing expansion, to allow it to support twelve companies to develop their manufacturing at scale, up from the current five. (onenucleus.com)
  • The co-location of SBC and the Cell and Gene Therapy Catapult in Stevenage has allowed resident companies to benefit from close proximity to a full supply chain, from research and development to manufacturing. (onenucleus.com)
  • That Stevenage Bioscience Catalyst is continuing to expand its world-class cell and gene therapy capabilities with the opening of the SBC Spark Building is great for our business," said Garry Menzel, Ph.D., President and Chief Executive Officer of TCR 2 Therapeutics. (onenucleus.com)
  • The foresight and ongoing commitment of the Government to tap into the huge potential of the local talent pool has made the Campus an ideal location for us as we can partner locally with the Cell and Gene Therapy Catapult for our manufacturing needs as we expand our presence in the UK. (onenucleus.com)
  • This includes UK Government support provided through the Cell and Gene Therapy Catapult (£55m), Industrial Strategy Challenge Fund (£12m), Innovate UK grant funding (£15m) and Hertfordshire LEP funding (£4.1m), which helped to unlock further European Regional Development Funding (£3.6m). (onenucleus.com)
  • They have designed ioCRISPR-Ready Cells to significantly reduce the time investment required to produce gene knockouts and run CRISPR screens in human iPSC-derived cells. (onenucleus.com)
  • Join this webinar to learn more about the alteration of host gene expression as a result of COVID-19 infection, and novel laboratory techniques designed to evaluate this at a single-cell resolution. (bucher.ch)
  • Single-cell RNA sequencing (scRNA-seq) is an increasingly powerful technology that enables analysis of gene expression in individual cells. (biomedcentral.com)
  • 2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)
  • It maintains a controlled and balanced environment, ensuring that the cell can carry out its activities efficiently. (the-one.world)
  • A critical role of autophagy was identified in rat nucleus pulposus (NP) cells: Inhibition of autophagy suppresses, while activation of autophagy enhances, the catabolic effect of cytokines. (spandidos-publications.com)
  • Upon inhibition of nuclear export, FKHR accumulates in the nucleus. (broadinstitute.org)
  • To our knowledge, this is the first report of cloned mammalian offspring originating from nonviable cells. (cnrs.fr)
  • Furthermore, autophagy of AF cells may be secondary to endoplasmic reticulum stress ( 12 , 13 ). (spandidos-publications.com)
  • packages molecules processed by the endoplasmic reticulum to be transported out of the cell. (medlineplus.gov)
  • However, an animal created through this technique would not be a precise genetic copy of the source of its nuclear DNA because each clone derives a small amount of its DNA from the mitochondria of the egg (which lie outside the nucleus) rather than from the donor of cell nucleus. (who.int)
  • Thus, the clone would be genetically identical to the nucleus donor only if the egg came from the same donor or from her maternal line. (who.int)
  • Reproducibly generate high-quality suspensions of nuclei from a wide range of tissues. (bucher.ch)
  • Solid tissues need to be dissociated to release individual cells suitable for 10x Genomics Chromium scRNA-seq. (biomedcentral.com)
  • Researchers can now easily obtain suspensions of nuclei or high-viability cells for a wide range of single-cell analyses. (bucher.ch)
  • The Singulator™ 100 enables rapid and hands-off tissue dissociations, making it easy for researchers to reproducibly prepare suspensions of nuclei or highly viable cells from small samples in high yield, for a wide range of single-cell analyses. (bucher.ch)
  • This study was designed to examine whether the unconventional spicing of XBP1 mRNA could occur in the nucleus and its possible biological relevance. (mdpi.com)
  • We use RT-PCR reverse transcription system and the expand high fidelity PCR system to detect spliced XBP1 mRNA, and fraction cells to determine the location of the unconventional splicing of XBP1 mRNA. (mdpi.com)
  • Demonstration of a dynamic, transcription-dependent organization of pre-mRNA splicing factors in polytene nuclei. (rupress.org)
  • We describe the dynamic organization of pre-mRNA splicing factors in the intact polytene nuclei of the dipteran Chironomus tentans. (rupress.org)
  • The animal cell with a nucleus, one of the greatest advancements in the realm of life, is like a sophisticated control center that drives the wonders of living organisms. (the-one.world)
  • In approximately 20% of organisms only 1 nucleus is present. (cdc.gov)
  • The term applies not only to entire organisms but also to copies of molecules (such as DNA) and cells. (who.int)
  • The bench-top Singulator System and its single-use cartridges enable reproducible, rapid and hands-off tissue dissociations into single-cell or nuclei suspensions. (bucher.ch)
  • The nucleus contains nearly all of the cell's DNA, surrounded by a network of fibrous intermediate filaments called the nuclear matrix, and is enveloped in a double membrane called the nuclear envelope. (wikipedia.org)
  • In most stem cells, the nuclear envelope is smooth, but wrinkles can occasionally form in its thin surface - sometimes taking on unexpected shapes. (alleninstitute.org)
  • To infect cells productively, HIV-1 must traverse the nuclear envelope to enable integration of the viral DNA into the genomic DNA of host cells. (phys.org)
  • MX2, which is localized at the cytoplasmic face of the nuclear envelope, inhibits infection by blocking the nuclear import of HIV-1 DNA and preventing its accumulation within the nucleus. (phys.org)
  • Therefore, by maintaining an efficient transport of the molecules into and out of the nucleus and by preserving the organisation of the nuclear envelope, the communication inside the cell is optimised and cells can adapt more quickly to the ever-changing environment, making our skin more resilient. (cosmeticsbusiness.com)
  • Our interdisciplinary study reveals how changes in cell nuclei induced by cryoprotectants affect the ability of cells to withstand freezing and thawing and how nuclei changes correlate with processes during freezing and thawing. (muni.cz)
  • The initial stages of DDD correlate with reduced cellularity, aberrant cell function, loss of proteoglycans and concomitant tissue dehydration [ 6 ]. (biomedcentral.com)
  • In eukaryotes the nucleus in many cells typically occupies 10% of the cell volume. (wikipedia.org)
  • Delivery of oncolytic adenovirus into the nucleus of tumorigenic cells by tumor microparticles for virotherapy. (stembook.org)
  • To investigate tumor necrosis factor α (TNF α) and interleukin-1β (IL-1 β) regulation of CCL3 expression in nucleus pulposus (NP) cells and in macrophage migration. (shu.ac.uk)
  • Ho CY, Maleki Z. Giant cell tumor of tendon sheath: cytomorphologic and radiologic findings in 41 patients. (ctsicn.org)
  • The study, HIV-1-induced nuclear invaginations mediated by VAP-A, ORP3, and Rab7 complex explain infection of activated T cells , was the result of a collaboration of researchers from Touro University Nevada College of Osteopathic Medicine, Touro College of Osteopathic Medicine in New York, researchers from the Biotechnology Center (BIOTEC) of TUD Dresden University of Technology in Germany, and researchers from Italy. (israelnationalnews.com)
  • Growing evidence has persuaded some researchers, however, that the nucleus might have arisen through a symbiotic partnership much like the one believed to have produced mitochondria. (uncommondescent.com)
  • Researchers from Tokyo Metropolitan University have discovered how the sponginess and stickiness of stem cell nuclei controls how they "differentiate" into specialized cells. (scitechdaily.com)
  • Allen Institute for Cell Science researchers photograph thousands and thousands of different cells under the microscope. (alleninstitute.org)
  • Researchers can get a more unbiased and complete view of how the cell nucleus is structured," Ma said. (cmu.edu)
  • Systematic comparison of recovered cell types and their transcriptional profiles across the workflows has highlighted protocol-specific biases and thus enables researchers starting single-cell experiments to make an informed choice. (biomedcentral.com)
  • Through a process called mitosis, cells can create exact copies of themselves, ensuring the growth and maintenance of the organism. (the-one.world)
  • 12 In human cells, the diameter of the nucleus is approximately six micrometres (µm). (wikipedia.org)
  • A study published in the journal Nature Communications has identified a new pathway that human immunodeficiency virus (HIV) uses to enter the nucleus of a healthy cell, where it can then replicate and go on to invade other cells. (israelnationalnews.com)
  • Early Alzheimer's disease pathology in human cortex involves transient cell states. (harvard.edu)
  • An atlas of healthy and injured cell states and niches in the human kidney. (harvard.edu)
  • A team led by Associate Professor Hiromi Miyoshi of Tokyo Metropolitan University have been looking at the nuclei of human mesenchymal stem cells, a type of cell that can mature (or "differentiate") into a wide range of cell types, including muscle, fat, bone, and cartilage. (scitechdaily.com)
  • This was the first time that the viscoelasticity of nuclei was tracked through the whole differentiation process in human stem cells. (scitechdaily.com)
  • Reference: "Intranuclear mesoscale viscoelastic changes during osteoblastic differentiation of human mesenchymal stem cells" by Kojiro Matsushita, Chiharu Nakahara, Shun Kimura, Naoya Sakamoto, Satoshi Ii and Hiromi Miyoshi, 25 November 2021, The FASEB Journal . (scitechdaily.com)
  • Herein we elaborated effects of TGF-β1 on TJs of primary human bronchial epithelial cells. (springer.com)
  • The cells these scientists work with are human stem cells, derived from an adult donor's skin cells and engineered to revert to a more "naive" state. (alleninstitute.org)
  • The mechanisms of immune privilege in human nucleus pulposus (NP) remain unclear. (medsci.org)
  • We aimed for addressing the role of FasL expression in human intervertebral disc degeneration (IDD) and immune privilege in terms of the interaction between NP cells and immunocytes via the FasL-Fas machinery. (medsci.org)
  • FCM analysis found that human NP cells with increased FasL expression resulted in significantly increased apoptosis ratio of macrophages and CD8 + T cells. (medsci.org)
  • Moreover, studies with human disc cells, especially focusing on nucleus pulposus (NP) cells, are seldom found in the literature. (medsci.org)
  • Finally, IKKβ knockdown was used to further confirm the effect of the NF‑κB signal on human NP cells autophagy, and the data showed that IKKβ knockdown upregulated the autophagy of NP cells during inflammatory conditions. (spandidos-publications.com)
  • In this study, we aimed to investigate whether phenotypically distinct clonal cell lines representing different subpopulations in the human NP could be generated using immortalization strategies. (biomedcentral.com)
  • NP cells were immortalized by simian virus 40 large T antigen (SV40LTag) and human telomerase reverse transcriptase expression. (biomedcentral.com)
  • The human body is composed of trillions of cells. (medlineplus.gov)
  • The National Human Genome Research Institute's Talking Glossary provides a definition of a cell as well as an illustration and video. (medlineplus.gov)
  • Taken together, our data provide proof of principle that direct neural conversion can take place in the adult rodent brain when using transplanted human cells or endogenous mouse cells as a starting cell for neural conversion. (lu.se)
  • In addition, because the cells do not pass a stem cell intermediate, direct neural conversion has the potential to be performed in vivo. (lu.se)
  • It also reveals cell types more abundant either in the cold or warm dissociations that may represent populations that require gentler or harsher conditions to be released intact. (biomedcentral.com)
  • The size of the nucleus is correlated to the size of the cell, and this ratio is reported across a range of cell types and species. (wikipedia.org)
  • While some techniques only perform the task of cell nuclei segmentation and quantification, techniques that are capable of further detecting and classifying abnormal tumors (cell nuclei) that cause various types of cancer have also been proposed. (hindawi.com)
  • Overall, snmC-seq allows for the large-scale multiplex sequencing of sorted neuronal nuclei that is needed to gain insight into the diverse cell types of the brain. (epigenie.com)
  • There are hundreds, if not thousands, of types of brain cells that have different functions and behaviors and it's important to know what all these types are to understand how the brain works. (epigenie.com)
  • We identified changes of cell nuclei states caused by different types of cryoprotectants and associate them with alterations of the freeze/thaw process in cells. (muni.cz)
  • The characterization of both metastable and fully committed embryonic stem cells (ESC) - pluripotent cells that are able to differentiate into almost all cell types in the body - is highly elusive. (illinois.edu)
  • However, optimal dissociation needs to achieve a balance between releasing cell types that are difficult to dissociate while avoiding damage to those that are fragile. (biomedcentral.com)
  • Each of the approaches mentioned above introduces specific biases and artifacts that can manifest themselves in altered transcriptional profiles or altered representation of cell types. (biomedcentral.com)
  • Cells also contain the body's hereditary material and can make copies of themselves. (medlineplus.gov)
  • They have their own genetic material, separate from the DNA in the nucleus, and can make copies of themselves. (medlineplus.gov)
  • But what about individual cells in our bodies? (uncommondescent.com)
  • There are certain bodies resembling cells in all respects except in the absence of a nucleus. (sacklunch.net)
  • Diagnosis is usually based on a complete blood count and peripheral smear, which usually shows a macrocytic anemia with anisocytosis and poikilocytosis, large oval red blood cells (macro-ovalocytes), Howell-Jolly bodies (residual fragments of the nucleus), hypersegmented neutrophils, and reticulocytopenia. (msdmanuals.com)
  • HIV infection requires the virus to enter a cell and gain access to the well-guarded nucleus in order for the viral components to be integrated into the healthy cell's DNA. (israelnationalnews.com)
  • He points to the "viral factories" that giant viruses build inside prokaryotic cells. (uncommondescent.com)
  • PL: nuclei) is a membrane-bound organelle found in eukaryotic cells. (wikipedia.org)
  • It is believed that TGF-β1 signalling is involved in controlling epithelial cell proliferation, to drive epithelial-mesenchymal transition (EMT) and it plays a major role in lung fibrosis and emphysema. (springer.com)
  • 649 Together, these membranes serve to separate the cell's genetic material from the rest of the cell contents, and allow the nucleus to maintain an environment distinct from the rest of the cell. (wikipedia.org)
  • They must enter a "host" cell and use that cell's machinery to replicate its genetic material, and therefore multiply. (sciencedaily.com)
  • Multiple tissue preparation protocols are compatible with Chromium, but the protocol of choice should ideally maintain RNA integrity and cell composition of the original tissue. (biomedcentral.com)
  • Cell Suspension Culture or Adherent Surface: Which Is Right for Your Lab? (corning.com)
  • HeLa, mouse L, and CHO cells were grown in suspension culture. (harvard.edu)
  • In this case, samples need to be preserved either as an intact tissue or in a dissociated form as a single-cell suspension. (biomedcentral.com)