• Stem and progenitor cells can be taken from the pelvis, at the iliac crest, using a needle and syringe. (wikipedia.org)
  • It was used extensively in early studies, but is now considered to measure more mature progenitor or transit-amplifying cells rather than stem cells[citation needed]. (wikipedia.org)
  • Also making up a part of the hematopoietic system are short-term repopulating progenitor cells, which give rise to lineage-specific cell types. (stemcell.com)
  • Collectively, these are referred to as hematopoietic stem and progenitor cells (HSPCs). (stemcell.com)
  • The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
  • Colonies derived from different types of progenitor cells are classified and counted based on morphological and phenotypic criteria. (stemcell.com)
  • CD34 is the most commonly used cell surface marker to identify human HSPCs as it is expressed on HSCs, in addition to both multipotent and more differentiated progenitor cells of individual blood cell lineages. (stemcell.com)
  • Hematopoietic stem cell transplantation (HSCT) is a broad term that covers the transplantation of blood progenitor/stem cells from any source. (drugdiscoverynews.com)
  • In this study, we observed that the expression of pseudouridine (Ψ) synthase 10 is increased in aged hematopoietic stem and progenitor cells (HSPC) and enforced protein of Ψ synthase 10 (PUS10) recapitulates the phenotype of aged HSC, which is not achieved by its Ψ synthase activity. (haematologica.org)
  • Bone marrow stem cells are targets for benzene-induced cytotoxicity and DNA damage that could result in changes to the genome of these progenitor cells, thereby leading to hematopoietic disorders and cancers. (rti.org)
  • Human bone marrow CD34(+) hematopoietic progenitor cells (HPC) were exposed in vitro to 1,4-BQ to assess cytotoxicity, genotoxicity, and DNA damage responses and the molecular mechanisms associated with these events. (rti.org)
  • Myeloid angiogenic cells (MAC) derive from hematopoietic stem/progenitor cells (HSPCs) that are mobilized from the bone marrow. (hindawi.com)
  • Such myeloid angiogenic cells (MAC) [ 1 ] of bone marrow origin were known as endothelial progenitor cells (EPCs). (hindawi.com)
  • However, the features initially ascribed to endothelial progenitor cells, i.e ., phenotype, expression of endothelial markers, and ability to form tube-like structures on Matrigel, can be mimicked by blood monocytes [ 3 ]. (hindawi.com)
  • Cells previously known as endothelial progenitor cells are considered now to be rather myeloid angiogenic cells than endothelial precursors [ 4 ]. (hindawi.com)
  • In our study, we worked on cells derived from the CD34 + hematopoietic stem and progenitor cells, enriched with CD133 + population, and used culture media promoting growth of hematopoietic progenitors. (hindawi.com)
  • Gene editing of the erythroid-specific BCL11A enhancer in hematopoietic stem and progenitor cells (HSPCs) from sickle cell disease (SCD) patients induces fetal hemoglobin (HbF) without detectable toxicity as assessed by mouse xenotransplant. (physiciansweekly.com)
  • Notch activation in human CD34+ hematopoietic stem/progenitor cells (HSPCs) by treatment with Delta1 ligand has enabled clinically relevant ex vivo expansion of short-term HSPCs. (confex.com)
  • We observed enhanced progenitor cell activity within the CD34+ cell population treated with Delta1 in hypoxia, but the benefits provided by low-oxygen cultures were most notable in the primitive HSC compartment. (confex.com)
  • Most clusters could be computationally assigned to a defined hematopoietic subpopulation, including progenitor cells (clusters 0 to 4) and a single transcriptionally defined HSC population (cluster 5). (confex.com)
  • The ability to genetically manipulate hematopoietic stem and progenitor cells (HSPCs) in particular has significantly advanced our understanding of the mechanisms that regulate hematopoiesis and is contributing to the development of novel cellular therapies. (stemcell.com)
  • Progenitor cell function may be measured post-modification by in vitro CFU assays. (stemcell.com)
  • As a first step toward the goal of defining the effects of DP treatment on haematopoiesis of preterm infants, we designed this in vitro study to determine whether DP affects the clonal growth of erythroid or granulocyte-macrophage progenitor cells in preterm infants, and if the preterm infant's progenitors respond to DP in a different manner from those of adults. (bmj.com)
  • 5 In this study we started with a highly enriched population of CD34+ progenitor cells 6 obtained by incubating the mononuclear fraction of blood and bone marrow with biotinylated anti-CD34 antibodies and running the CD34 labelled cells over an avidin column (Cellpro, Inc., Bothell, Washington, USA). (bmj.com)
  • The aryl hydrocarbon receptor directs hematopoietic progenitor cell expansion and differentiation. (umassmed.edu)
  • Moreover, we observed molecular changes in cell cycle, MYC and mTOR signaling in both HSCs, and progenitor subsets. (lu.se)
  • This review outlines current protocols and cell markers used in flow cytometric analysis of hematopoietic stem and progenitor cell populations . (bvsalud.org)
  • Developmental processes, in particular those of stem and progenitor cell commitments, are not only topical but also particularly suitable for kinetic modeling due to the finite number of key genes involved in cellular decisions. (lu.se)
  • Stem and progenitor cell commitment processes have been subject to intense experimental studies over the last decade with some emphasis on embryonic and hematopoietic stem cells. (lu.se)
  • The hematopoietic tissue contains cells with long-term and short-term regeneration capacities and committed multipotent, oligopotent, and unipotent progenitors. (wikipedia.org)
  • We examined hematopoietic stem cells at four stages of differentiation, lineage-committed progenitors, and mature myeloid and lymphoid cells. (nih.gov)
  • With this method, we will provide the first in vivo description of the human hematopoietic tree and identify new progenitors and key molecular factors involved in human hematopoiesis. (europa.eu)
  • Endothelial progenitors should be devoid of hematopoietic markers and able to form vessels both in vitro and in vivo . (hindawi.com)
  • 02:14 progenitors that make red blood cells. (yale.edu)
  • Additionally, edited CD34 + cells can be further cultured in conditions stimulating lineage-specific differentiation by combining StemSpan™ media with expansion supplements designed for the expansion and differentiation of progenitors to erythroid, myeloid, megakaryocyte, natural killer (NK), or T cell lineages. (stemcell.com)
  • The in vitro effect of dexamethasone on the clonal growth of haematopoietic progenitors in preterm infants was investigated. (bmj.com)
  • Molecular changes underlying stem cell differentiation are of fundamental interest. (nih.gov)
  • This study reveals effects of cell cycle progression on the expression of lineage specific genes in precursor cells, and suggests that hematopoietic stress changes the balance of renewal and differentiation in these homeostatic cells. (nih.gov)
  • The telomerase-expressing somatic cells observed in this study are not thought to be immortal, and expression was not correlated with cell cycle distribution or differentiation state. (nih.gov)
  • Our microsatellite barcoding method exploits the fact that microsatellites in various loci undergo length changes during cell division, to create a natural barcode for each cell, and reconstruct the overall differentiation tree using a phylogenetic algorithm. (europa.eu)
  • Therefore, we will provide insights into both cell lineage and the molecular mechanisms involved in differentiation. (europa.eu)
  • More generally it will impact our understanding of stem cell differentiation for which hematopoiesis is used as an exemplar. (europa.eu)
  • Cell markers, or surface antigens, are molecules located on a cell's membrane used to identify specific cell types, their lineage, and their stage in the differentiation process according to the presence or absence of the expression of defined markers. (stemcell.com)
  • Aged hematopoietic stem cells (HSC) exhibit compromised reconstitution capacity and differentiation-bias towards myeloid lineage, however, the molecular mechanism behind it remains not fully understood. (haematologica.org)
  • Indoles derivatives are ligands of the receptor Aryl Hydrocarbon (AhR), which regulates the activation and differentiation of immune cells and could therefore contribute to limiting the risk of immune reaction to allogeneic origin of GVHD. (pasteur.fr)
  • I found that rRNA transcription was downregulated during hematopoietic differentiation in both human leukemia cell line and mouse hematopoietic stem cells. (nii.ac.jp)
  • In response to the inhibition of rRNA transcription, nucleolar protein MYBBP1A was translocated from the nucleolus to the nucleoplasm and interacted with c-Myc, which is one of the master regulator of hematopoietic differentiation. (nii.ac.jp)
  • I also found that hematopoietic differentiation was sufficiently induced by specific suppression of rRNA transcription using actinomycin D or siRNA for TIF-IA. (nii.ac.jp)
  • These results suggest that the downregulation of rRNA transcription accelerates cellular differentiation at least in hematopoietic cells. (nii.ac.jp)
  • Specific niches within the bone marrow are discussed, as are metabolic processes that contribute to stem cell self-renewal and differentiation, as well as the role of hematopoietic stem cells outside of the bone marrow at physiologic steady state . (bvsalud.org)
  • Hematopoietic stem cell transplantation (HSCT) involves the intravenous infusion of hematopoietic stem cells in order to reestablish blood cell production in patients whose bone marrow or immune system is damaged or defective. (medscape.com)
  • Cells for HSCT may be obtained from the patient himself or herself (autologous transplant) or from another person, such as a sibling or unrelated donor (allogeneic transplant) or an identical twin (syngeneic transplant). (medscape.com)
  • The National Marrow Donor Program (NMDP), founded in 1986, and the World Marrow Donor Association (WMDA), founded in 1988, were established to (1) locate and secure appropriate unrelated-donor HSCT sources for patients by promoting volunteer donation of bone marrow and peripheral blood stem cells in the community and (2) promote ethical practices of sharing stem cell sources by need, rather than by geographic location of the donor. (medscape.com)
  • Among the conditions HSCT can treat are: acute myeloid leukemia, acute lymphoblastic leukemia, chronic myeloid leukemia, chronic lymphocytic leukemia, myeloproliferative disorders, myelodysplastic syndromes, multiple myeloma, non-Hodgkin lymphoma, Hodgkin disease, aplastic anemia and pure red-cell aplasia-but this list is not exhaustive. (drugdiscoverynews.com)
  • Other groups are also working on cell therapies for the treatment of poor graft function after HSCT. (drugdiscoverynews.com)
  • Their ongoing Phase 2 study is evaluating whether infusion of mesenchymal stem cells (MSCs) can treat steroid-resistant acute graft-versus-host disease (GVHD) or poor graft function after HSCT. (drugdiscoverynews.com)
  • The year 2020 marked the 30th anniversary of the Nobel Prize in Medicine awarded to E. Donnall Thomas for the development of allogeneic hematopoietic stem cell transplantation (allo-HSCT) to treat hematologic malignancies and other blood disorders. (broadinstitute.org)
  • Body iron disorders have been reported after myeloablative conditioning in patients undergoing hematopoietic stem cell transplantation (HSCT). (karger.com)
  • There is a paucity of data examining the tolerance and outcome of hematopoietic stem cell transplantation (HSCT) for relapse after tositumomab/iodine-131 tositumomab. (cancernetwork.com)
  • There is a paucity of data examining the tolerance andoutcome of hematopoietic stem cell transplantation (HSCT) for relapse aftertositumomab/iodine-131 tositumomab. (cancernetwork.com)
  • Five patients received autologous HSCT: threemarrow and two peripheral blood stem cells. (cancernetwork.com)
  • The potential role of elastin in patients with myeloid leukemia treated with hematopoietic stem cell transplantation (HSCT) has not been investigated so far. (medscimonit.com)
  • Akari's pipeline includes a Phase 3 clinical trial program investigating nomacopan for severe pediatric hematopoietic stem cell transplant-related thrombotic microangiopathy (HSCT-TMA). (texomashomepage.com)
  • Akari has been granted Orphan Drug, Fast Track and Rare Pediatric Disease designations from the FDA for nomacopan for the treatment of pediatric HSCT-TMA and orphan drug designation from the European Commission for treatment in hematopoietic stem cell transplantation. (texomashomepage.com)
  • This study aimed to know the perceptions and experiences of family companions facing illness and transplantation of hematopoietic stem cells (HSCT) in light of the theory of anticipatory grief. (bvsalud.org)
  • Hematopoietic stem cells (HSCs) are the stem cells that give rise to other blood cells. (wikipedia.org)
  • In vertebrates, the very first definitive HSCs arise from the ventral endothelial wall of the embryonic aorta within the (midgestational) aorta-gonad-mesonephros region, through a process known as endothelial-to-hematopoietic transition. (wikipedia.org)
  • HSCs make up a very small population of the hematopoietic system, however, these cells are invaluable as they have the potential to give rise to all mature blood and immune cell types and sustain life-long blood production. (stemcell.com)
  • HSCs specifically are defined by their ability for self-renewal and to reconstitute the entire hematopoietic system following transplantation. (stemcell.com)
  • Haematopoietic stem cells (HSCs) are a self-renewing population of cells that continuously replenish all blood and immune cells during the lifetime of an individual. (ca.gov)
  • HSCs are used clinically to treat a wide array of diseases, including acute leukaemias and congenital blood disorders, but obtaining suitable numbers of cells and finding immune-compatible donors remain serious problems. (ca.gov)
  • These difficulties have led to an interest in the conversion of embryonic stem cells or induced pluripotent stem cells into HSCs, which is not possible using current methodologies. (ca.gov)
  • Notch signalling downstream of Dlc and Dld is earlier than, and distinct from, known cell-autonomous requirements for Notch, strongly suggesting that novel Notch-dependent relay signal(s) induce the first HSCs in parallel to other established pathways. (ca.gov)
  • Bone marrow hematopoietic stem cells (HSCs) are vital for the maintenance of hematopoiesis. (irbbarcelona.org)
  • In inbred mice housed in gnotobiotic facilities, the top of the hematopoietic hierarchy is occupied by dormant HSCs, which reversibly exit quiescence during stress. (irbbarcelona.org)
  • Long-term hematopoietic stem cells (LT-HSCs) maintain hematopoietic output throughout an animal's lifespan. (biorxiv.org)
  • Here, we show that young and aged LT-HSCs respond differently to inflammatory stress, such that aged LT-HSCs produce a cell-intrinsic, myeloid-biased expression program. (biorxiv.org)
  • Using single-cell RNA-seq, we identify a myeloid-biased subset within the LT-HSC population (mLT-HSCs) that is much more common amongst aged LT-HSCs and is uniquely primed to respond to acute inflammatory challenge. (biorxiv.org)
  • Aged LT-HSCs demonstrate a cell-intrinsic myeloid bias during inflammatory challenge. (biorxiv.org)
  • In summary, we find that edited HSCs can persist for at least 101 weeks post-transplant, and biallelic edited HSCs provide substantial HbF levels in PB red blood cells, together supporting further clinical translation of this approach. (physiciansweekly.com)
  • Umbilical cord blood (UCB) is a valuable source of hematopoietic stem cells (HSCs) and potential alternative for bone marrow transplantation for patients who lack human leukocyte antigen (HLA)-matched donors. (wjgnet.com)
  • However, sustained engraftment of the expanded cells was not observed after transplantation, suggesting ineffective expansion of hematopoietic stem cells with long-term repopulating activity (LTR-HSCs). (confex.com)
  • At optimal coating densities of Delta1, the frequency of LTR-HSCs measured by limiting dilution analysis 16 weeks after transplantation into NSG mice was 4.9- and 4.2-fold higher in hypoxic cultures (1 in 1,586 CD34+ cells) compared with uncultured cells (1 in 7,706) and the normoxia group (1 in 5,090), respectively. (confex.com)
  • Conversely, we observed no difference in expression of the homing CXCR4 receptor between cells cultured under normoxic and hypoxic conditions, indicating that hypoxia increased the absolute numbers of LTR-HSCs but not their homing potential after transplantation. (confex.com)
  • To corroborate these findings molecularly, we performed transcriptomic analyses and found significant upregulation of a distinct HSC gene expression signature in cells cultured with Delta1 in hypoxia (Fig. A). Collectively, these data show that hypoxia supports a superior ex vivo expansion of human HSCs with LTR activity compared with normoxia at optimized densities of Delta1. (confex.com)
  • To clarify how hypoxia improved Notch-mediated expansion of LTR-HSCs, we performed scRNA-seq of CD34+ cells treated with Delta1 under normoxic or hypoxic conditions. (confex.com)
  • Hematopoietic stem cells (HSCs) enable hematopoietic stem cell transplantation (HCT) through their ability to replenish the entire blood system. (lu.se)
  • Subsequent transplantation of the cells into lethally irradiated mice results in engraftment of the animals with donor haematopoietic tissue containing the bacterial gene. (nature.com)
  • Test the activities of the vectors in hematopoietic stem cells from healthy donor in culture and by growing in immune deficient mice. (ca.gov)
  • For an allogeneic stem cell transplant , after the bone marrow is destroyed, the patient receives blood-forming stem cells from another person - the donor," the American Cancer Society explained. (curetoday.com)
  • Understanding how hematopoietic stem cells are made during embryonic development is important because it could teach us how to make such cells in the laboratory, and possibly allow circumvention of donor immune compatibility issues. (ca.gov)
  • Graft against the host (GVHD) is a major complication that may occur after a allograft, and is characterized by tissue damage induced by donor immune cells which react against those of the recipient. (pasteur.fr)
  • Recent advances in the understanding of immunological mechanisms of GVHD have shown that activation of donor immune cells is tightly regulated by the microenvironment tissue or digestive microbiota changes after transplantation. (pasteur.fr)
  • We used G-CSF-mobilized CD34 + HSPCs, FACS-sorted from healthy donor peripheral blood mononuclear cells (PBMCs). (hindawi.com)
  • We also analyse the engraftment of donor stem cells into non-conditioned and conditioned hosts, quantifying the impact of different treatment scenarios. (uzh.ch)
  • We predict the time taken for mutant clones to expand within a host, as well as chimerism levels that can be expected following transplantation therapy, and the probability that a preconditioned host is reconstituted by donor cells. (uzh.ch)
  • A study by MacMillan et al reported that alternative donor hematopoietic cell transplantation had a high success rate in patients with Fanconi anemia who did not have a history of opportunistic infections or transfusions and who underwent conditioning with single fraction total body irradiation 300 cGy, cyclophosphamide, fludarabine, and antithymocyte globulin. (medscape.com)
  • Stem cells are removed from another person, called a donor. (medlineplus.gov)
  • Leukapheresis -- First, the donor is given several days of shots to help stem cells move from the bone marrow into the blood. (medlineplus.gov)
  • The red blood cells are returned to the donor. (medlineplus.gov)
  • Hematopoietic stem cells give rise to different types of blood cells, in lines called myeloid and lymphoid. (wikipedia.org)
  • Myeloid and lymphoid lineages both are involved in dendritic cell formation. (wikipedia.org)
  • Hematopoietic stem cells constitute 1:10,000 of cells in myeloid tissue. (wikipedia.org)
  • These factors may regulate myeloid versus lymphoid balance with age, and can potentially mitigate the long-term deleterious effects of inflammation that lead to hematopoietic pathologies. (biorxiv.org)
  • One sentence summary Murine hematopoietic stem cells display transcriptional heterogeneity that is quantitatively altered with age and leads to the age-dependent myeloid bias evident after inflammatory challenge. (biorxiv.org)
  • Physiologic aging in both humans and mice leads to permanent changes in LT-HSC function, such as myeloid-biased hematopoietic output ( Akunuru and Geiger, 2016 ). (biorxiv.org)
  • Our study is aimed at investigating whether precursors of myeloid angiogenic cells (PACs) treated with known pharmaceuticals would produce media with better proangiogenic activity in vitro and if such media can be used to stimulate blood vessel growth in vivo . (hindawi.com)
  • Therefore, the cells we used should be considered as hematopoietic precursors of myeloid angiogenic cells and are referred as PACs. (hindawi.com)
  • Among 1462 patients who had allogeneic hematopoietic cell transplantation (HCT) between January 2000 and December 2005, 116 (7.9%) developed stage 3-4 gut GVHD. (nature.com)
  • Reduced mortality after allogeneic hematopoietic-cell transplantation. (nature.com)
  • Effect of race on outcomes after allogeneic hematopoietic cell transplantation for severe aplastic anemia. (aamds.org)
  • CD34 is a transmembrane phosphoglycoprotein that was first identified on HSPCs, but its expression is also present on other cell types, such as vascular tissue. (stemcell.com)
  • Additional markers can be used to distinguish HSPC subsets within the CD34+ population and isolate HSPCs with different engraftment abilities and capacities to expand or generate mature blood cells in culture. (stemcell.com)
  • citation needed] Hematopoietic stem cells can be identified or isolated by the use of flow cytometry where the combination of several different cell surface markers (particularly CD34) are used to separate the rare Hematopoietic stem cells from the surrounding blood cells. (wikipedia.org)
  • A significant increase in the percentage of micronucleated CD34(+) cells was detected in cultures treated with 1,4-BQ. (rti.org)
  • In addition, the p21 mRNA level was elevated in 1,4-BQ-treated cells, suggesting that human CD34(+) cells utilize the p53 pathway in response to 1,4-BQ-induced DNA damage. (rti.org)
  • These results show that human CD34(+) cells are sensitive targets for 1,4-BQ toxicity that use the p53 DNA damage response pathway in response to genotoxic stress. (rti.org)
  • CD34 + cells after six days in culture were stimulated with atorvastatin (AT), acetylsalicylic acid (ASA), sulforaphane (SR), resveratrol (RV), or metformin (Met) for 48 h. (hindawi.com)
  • EPCs are enriched in the population coexpressing CD34, CD133, and KDR with a low or absent expression of pan-hematopoietic marker CD45 [ 1 ]. (hindawi.com)
  • This Technical Bulletin provides instructions for CRISPR-Cas9 genome editing and maintenance of human CD34 + cells, including optimized pre- and post-editing culture conditions and methods to evaluate genome editing efficiency. (stemcell.com)
  • While CRISPR-Cas9 technology has been successfully applied in numerous cell lines, its application in primary human CD34 + cells has been hampered by challenges in efficient expression and delivery of CRISPR-Cas9 components. (stemcell.com)
  • The ArciTect™ CRISPR-Cas9 RNP is then prepared and delivered into CD34 + cells using electroporation, and cells are plated in StemSpan™ SFEM II supplemented with StemSpan™ CD34 + Expansion Supplement post-electroporation. (stemcell.com)
  • scRNA-seq on murine hematopoietic stem cells (HSC) and their progeny MPP1 separated the cells into 3 main clusters with distinct features: active, quiescent, and an un-characterized cluster. (nih.gov)
  • While murine hematopoiesis has been considered as resolved science, recent results using barcoding, which allows the tracing of single cells in vivo, clearly show that the topology of the murine hematopoietic tree needs revisions. (europa.eu)
  • The hematopoietic stem cells (CD34+ cells) are collected from the peripheral blood and used for autologous transplantation in patients with non-Hodgkin's lymphoma and multiple myeloma . (drugs.com)
  • The safety profile of venetoclax following allogeneic hematopoietic stem cell transplantation in patients with chronic lymphocytic leukemia appears to be comparable with other reports in clinical trials. (cancernetwork.com)
  • NOD-SCID-Gamma (NSG) mice) and measuring the presence of human blood cells in the blood or bone marrow (BM) after extended periods of engraftment, e.g., 20 weeks or longer. (stemcell.com)
  • Incomplete engraftment (poor graft function) requires the patient to undergo more transfusions of red cells or platelets. (drugdiscoverynews.com)
  • One trial is testing whether MSCs with or without peripheral blood stem cells could treat poor graft function and delayed platelet engraftment. (drugdiscoverynews.com)
  • The study, which involved 130 patients, also found that adding fludarabine to conditioning caused three-fold enhancement of hematopoietic cell engraftment. (medscape.com)
  • An acute graft-versus-host disease activity index to predict survival after hematopoietic cell transplantation with myeloablative conditioning regimens. (nature.com)
  • Prospective evaluation for upper gastrointestinal tract acute graft-versus-host disease after hematopoietic stem cell transplantation. (nature.com)
  • Lack of expression of lineage markers is used in combination with detection of several positive cell-surface markers to isolate hematopoietic stem cells. (wikipedia.org)
  • Cells with surface markers of long term HSC increased the expression of a group of TFs expressed highly in normal cycling MPP1 cells. (nih.gov)
  • Our technique, which is highly detailed with respect to current versions of microsatellite barcoding, provides high dimensional characterization of the cells and will allow the inference of important properties such as new phenotypic markers and key molecular regulators. (europa.eu)
  • Identification of Gene Expression-Based Prognostic Markers in the Hematopoietic Stem Cells of Patients With Myelodysplastic Syndromes. (stembook.org)
  • An infectious retrovirus vector has been used to transfer a bacterial gene encoding resistance to the neomycin analogue G418 into pluripotent haematopoietic stem cells present in explanted murine bone marrow tissue. (nature.com)
  • Develop lentiviral vectors carrying human alpha-globin gene for gene therapy of alpha thalassemia (AT) and perform initial tests in a cell line. (ca.gov)
  • Retroviral gene transfer to primitive normal and leukemic hematopoietic cells using clinically applicable procedures. (jci.org)
  • Clinical uses of gene transfer to bone marrow transplants require the establishment of a reproducible method for infecting large numbers of very primitive hematopoietic cells at high efficiency using cell-free retrovirus-containing media. (jci.org)
  • Marrow cells enriched greater than or equal to 500-fold for LTC-IC (1-2% pure) by flow cytometry showed gene transfer efficiencies of 27% when infected in a similar fashion over a shorter period (24 h), but in the presence of added soluble IL-3 and G-CSF without stromal feeders, and this increased to 61% when Steel factor was also present during the infection period. (jci.org)
  • Other uses of the CFU assay include studying the effects of stimulatory and inhibitory growth factors, screening novel compounds to predict potential toxicity to the hematopoietic system, and testing the effects of various in vitro manipulations (e.g. cell processing, cryopreservation, gene transduction, and transmission) on cellular products used in hematopoietic cell transplantation. (stemcell.com)
  • We found that gene edits persisted in peripheral blood (PB) and bone marrow (BM) for up to 101 weeks similarly for BCL11A enhancer or control locus (AAVS1) targeted cells. (physiciansweekly.com)
  • To assess the relative impact of normoxia and hypoxia on the HSC compartment, we performed gene set enrichment analysis (GSEA) of cells within HSC cluster 5 from each culture condition. (confex.com)
  • Such models mostly concern kinetics and include gene and protein interactions as well as cell population dynamics. (lu.se)
  • Southern analysis of DNA from the nonadherent cells produced by these LTC over a 6-wk period provided evidence of clonal evolution of LTC-IC in vitro. (jci.org)
  • These data demonstrate the feasibility of using cell-free virus preparations for infecting clinical marrow samples suitable for transplantation, as well as for further analysis of human marrow stem cell dynamics in vitro. (jci.org)
  • Concluding, high concentrations of atorvastatin stabilize tubes and enhance the paracrine angiogenic activity of human PAC cells in vitro . (hindawi.com)
  • 02:47 and red blood cells in vitro. (yale.edu)
  • Another CFU, the colony-forming unit-spleen (CFU-S), was the basis of an in vivo clonal colony formation, which depends on the ability of infused bone marrow cells to give rise to clones of maturing hematopoietic cells in the spleens of irradiated mice after 8 to 12 days. (wikipedia.org)
  • However, at least Id1 and Hes1 were significantly activated in both HSC and MPP1 cells in anemic mice. (nih.gov)
  • No significant alteration of hematopoietic homeostasis and HSC function is observed in young Pus10-/- mice, while aged Pus10-/- mice exhibit mild alteration of hematopoietic homeostasis and HSC function. (haematologica.org)
  • In vertebrates, the vast majority of hematopoiesis occurs in the bone marrow and is derived from a limited number of hematopoietic stem cells that are multipotent and capable of extensive self-renewal. (wikipedia.org)
  • Allogeneic hematopoietic stem cell (HSC) is a curative treatment used for many hematologic malignancies and congenital or acquired anomalies of hematopoiesis. (pasteur.fr)
  • Dr. Thomas, "father of bone marrow transplantation," first developed and reported this technique in 1957, and in the ensuing decades, this seminal study has impacted fundamental work in hematology and cancer research, including advances in hematopoiesis, stem cell biology, tumor immunology, and T-cell biology. (broadinstitute.org)
  • Hematopoietic stem cells and extramedullary hematopoiesis in the lungs. (bvsalud.org)
  • Hematopoietic stem cells are key players in hematopoiesis as the body maintains a physiologic steady state , and the signaling pathways and control mechanisms of these dynamic cells are implicated in processes from inflammation to cancer . (bvsalud.org)
  • Although the bone marrow is commonly regarded as the site of hematopoiesis and hematopoietic stem cell residence, these cells also circulate in the blood and reside in extramedullary tissues , including the lungs . (bvsalud.org)
  • Hematopoiesis in the lungs is a new and emerging concept, and discovering ways in which the study of lung -resident hematopoietic stem cells can be translated from murine models to patients will impact clinical treatment . (bvsalud.org)
  • Here we review kinetic modeling efforts applied to stem cell developmental systems with focus on hematopoiesis. (lu.se)
  • We highlight the future challenges including multi-scale models integrating cell dynamical and transcriptional models. (lu.se)
  • We assayed telomerase activity in single cells of the hematopoietic and immune systems. (nih.gov)
  • Hematopoietic stem cells in the bone marrow give rise to red blood cells and immune cells . (fightaging.org)
  • Aging of the hematopoietic system promotes various blood, immune, and systemic disorders and is largely driven by hematopoietic stem cell (HSC) dysfunction. (fightaging.org)
  • Hematopoietic stem cells are an important population of cells that continuously produce and replace red blood and immune system cells during life. (ca.gov)
  • Indications for hematopoietic cell transplantation and immune effector cell therapy: Guidelines from the American Society for Transplantation and Cellular Therapy. (msdmanuals.com)
  • The very first hematopoietic stem cells during (mouse and human) embryonic development are found in aorta-gonad-mesonephros region and the vitelline and umbilical arteries. (wikipedia.org)
  • In this research we describe a previously unknown set of molecular inputs that are required to make hematopoietic stem cells during embryonic development. (ca.gov)
  • The frequency of telomerase-expressing cells within each population was proportional to the frequency of cells thought to have self-renewal potential. (nih.gov)
  • This knowledge will allow us to test whether cells with different origins have different functions, a key question in immunology in particular during infection and inflammation, and it will help design ways of therapeutically manipulating the hematopoietic system. (europa.eu)
  • We found that during systemic cytopathic virus infection, hematopoietic cells were essential for production of IFN-I, inhibition of viral spread to peripheral organs, and limiting cell damage. (uzh.ch)
  • In a model of autoimmune diabetes induced by noncytopathic virus infection, hematopoietic cell-derived IFN-I was essential for CD8(+) T cell-dependent cytotoxicity in pancreatic beta-islet cells and induction of diabetes. (uzh.ch)
  • These data suggest that during systemic viral infection primarily hematopoietic cell-derived IFN-I controls viral replication and viral-induced disease. (uzh.ch)
  • Examples of secondary immunodeficiency include HIV infection, hematopoietic malignancies, treatment with radiation, and treatment with immunosuppressive drugs. (cdc.gov)
  • For many cancers, the donor's white blood cells may attack any remaining cancer cells, which are seen as foreign, similar to when white cells attack bacteria or viruses when fighting an infection. (medlineplus.gov)
  • Hematopoietic cell transplant is currently the only known cure for myelodysplastic syndrome. (curetoday.com)
  • Stem cell transplant side effects can include low blood counts and graft-versus-host disease and can potentially be life-threatening, according to the American Cancer Society. (curetoday.com)
  • Under immunosuppression conditions, all infectious foci can be activated and, for this reason, all active infections in hematopoietic stem cell pre-transplant patients must be eradicated prior to transplant so as to prevent or reduce the risk of systemic complications in these patients. (bvsalud.org)
  • Aim: Present a case report of dental care provided to a non-Hodgkin lymphoma patient with an urgent need for hematopoietic stem cell transplant. (bvsalud.org)
  • Guidelines for preventing opportunistic infections among hematopoietic stem cell transplant recipients. (cdc.gov)
  • A bone marrow transplant is a procedure to replace damaged or diseased bone marrow with healthy bone marrow stem cells. (medlineplus.gov)
  • The stem cells are frozen and stored until they are needed for a transplant. (medlineplus.gov)
  • A stem cell transplant is usually done after chemotherapy and radiation is complete. (medlineplus.gov)
  • Haematopoietic stem cell transplants have been performed in more than 1 500 000 patients (both autologous and allogeneic) to date.1 Although haematological cancers remain the main indication, haematopoietic stem cell transplants are increasingly considered in the treatment of non-malignant disorders and genetic diseases such as haemoglobinopathies (sickle cell anaemia, thalassaemia) that can benefit greatly from this type of transplant. (who.int)
  • Hematopoietic cell transplantation improved survival among high-risk genetic subgroups of patients with myelodysplastic syndrome, a recent study found. (curetoday.com)
  • Hematopoietic stem cell transplantation (bone marrow, cord blood, or peripheral blood stem cells) may cure aplastic anemia and prevent myelodysplastic syndrome or leukemia. (medscape.com)
  • Ablative (myeloablative) treatment -- High-dose chemotherapy, radiation, or both are given to kill any cancer cells. (medlineplus.gov)
  • These rare cells are the important element of bone marrow transplants, which are used to treat a variety of conditions including many forms of blood cancer. (ca.gov)
  • Among seven allogeneic transplants,three received matched related peripheral blood stem cells and four receivedmatched unrelated marrow (n = 3) or peripheral blood (n = 1) stem cells.Allogeneic recipients received tacrolimus and methotrexate for graft-vs-hostdisease prophylaxis. (cancernetwork.com)
  • The purpose is to deliver chemotherapy, immunotherapy, and/or radiation to eliminate malignancy, prevent rejection of new stem cells, and create space for the new cells. (medscape.com)
  • HCT is a procedure where healthy blood-forming cells are administered to replace a patient's cells destroyed by chemotherapy or radiation treatments. (curetoday.com)
  • using cells from bone marrow, blood, or less often, umbilical cord blood cells are given after high- or moderate-dose chemotherapy and/or radiation therapy. (msdmanuals.com)
  • Stem cells are removed from you before you receive high-dose chemotherapy or radiation treatment. (medlineplus.gov)
  • After high-dose chemotherapy or radiation treatments, your stems cells are put back in your body to make normal blood cells. (medlineplus.gov)
  • Hematopoietic stem cells can replenish all blood cell types (i.e., are multipotent) and self-renew. (wikipedia.org)
  • Lymphoid cells include T cells, B cells, natural killer cells, and innate lymphoid cells. (wikipedia.org)
  • Nature Cell Biology 2022). (irbbarcelona.org)
  • Department of Cell and Molecular Biology, St. Jude Children's Research Hospital, Memphis, United States of America. (physiciansweekly.com)
  • CRISPR-Cas9, an RNA-guided genome editing technology, is revolutionizing cell biology due to the ease and efficiency by which it enables genetic manipulation of mammalian cells. (stemcell.com)
  • As cell and molecular biology is becoming increasingly quantitative, there is an upsurge of interest in mechanistic modeling at different levels of resolution. (lu.se)
  • Pluristem Therapeutics, based in Haifa, Israel, is currently recruiting patients in the United States and Israel for its Phase 1 trial of PLX-R18 cells in this unmet need. (drugdiscoverynews.com)
  • The cells are also being evaluated in a variety of other hematologic indications. (drugdiscoverynews.com)
  • Alejo Rodriguez-Fraticelli, Ph.D - Group Leader , Quantitative Stem Cell Dynamics, Aging and Metabolism Programme. (irbbarcelona.org)
  • Each of those cell sources has specific advantages and disadvantages, and each has found particular clinical applications. (medscape.com)
  • It is used both by hematopoietic researchers and clinical labs to assess the potency of transplantation units of cord blood (CB) and hematopoietic cellular therapy products. (stemcell.com)
  • BCL11A enhancer edited hematopoietic stem cells persist in rhesus monkeys without toxicity. (physiciansweekly.com)
  • For erythroid colony forming unit (CFU-E) quantification, we used 2000 cells/ml, while for erythroid burst forming units (BFU-E), colony forming units mix (CFU-mix), and granulocyte-macrophage colony-forming unit's (CFU-GM), we used 7000 cells/ml. (bmj.com)
  • In May 2010, the Sixty-third World Health Assembly adopted resolution WHA63.22,1 in which it endorsed the updated WHO Guiding Principles on Human Cell, Tissue and Organ Transplantation and provided strategic directions to support progress in human organ, tissue and cell donation with the aim of maximizing the benefits of transplantation, meeting the needs of recipients, protecting donors and ensuring the dignity of all involved. (who.int)
  • Conditioned media from such cells were then used to stimulate human aortic endothelial cells (HAoECs) to enhance tube-like structure formation in a Matrigel assay. (hindawi.com)
  • Then, we used atorvastatin-stimulated cells or conditioned media from them in the Matrigel plug in vivo angiogenic assay. (hindawi.com)
  • Lineage-specific genes were differently expressed between cells, and correlated with the cell cycle stages with a specific augmentation of erythroid related genes in the G2/M phase. (nih.gov)
  • Most lineage specific TFs were stochastically expressed in the early precursor cells, but a few, such as Klf1, were detected only at very low levels in few precursor cells. (nih.gov)
  • Eventually these findings may help us discover the complete set of molecular controls necessary for making hematopoietic stem cells. (ca.gov)
  • Outcomes of corneal transplantation in Europe: report by the European Cornea and Cell Transplantation Registry. (who.int)
  • Cellular activation through pattern recognition receptors induces interferon production in a wide variety of hematopoietic and nonhematopoietic cell types, including dendritic cells, fibroblasts, hepatocytes, and cells of neuronal origin. (uzh.ch)
  • Stem cells are removed from a newborn baby's umbilical cord right after birth. (medlineplus.gov)
  • Umbilical cord blood cells are very immature so there is less of a need for perfect matching. (medlineplus.gov)
  • Genetic dissection of mesodermal commitment to the hematopoietic fates. (ca.gov)