ProliferationProteinNucleiAntigenTissueEndothelial cellsConstitutively expressedTissuesTumorReceptorApoptosisKappaEmbryonicEpithelial cellsGeneAssaysVectorsNucleusDifferentiationProgenitorA549CytoplasmicDrosophilaAntibodyTranscriptionCellularGenesExpressionHighly expressedUbiquitouslyMiceReplicationPronounced cytotoxicityMalignantEukaryoticTranslocationStem cell transplantRadioactiveChromatinMetabolismEmbryosInteractsLinesPromotesAssayNeuronsMarkersRegulationLymphomaPatternsCarcinomaMutationsFluorescenceHigh-throughputRolesSignificantly

Proliferation11

• C) Numerous neoplastic cells express the proliferation marker Ki67. (cdc.gov)
• It is believed that TGF-β1 signalling is involved in controlling epithelial cell proliferation, to drive epithelial-mesenchymal transition (EMT) and it plays a major role in lung fibrosis and emphysema. (springer.com)
• Combined treatment with selumetinib and a dose of palbociclib sufficient to reinforce G1 arrest in selumetinib-sensitive cells, but not to impair proliferation of resistant cells, delays the emergence of resistant colonies, meaning that escape from G1 arrest is critical in the formation of resistant clones. (babraham.ac.uk)
• Ly6C high MC presented downregulated co-stimulatory receptors (CD2, GITR, and TIM1) which direct immune cell proliferation, and upregulated co-stimulatory ligands (LIGHT and SEMA4A) which trigger antigen priming and differentiation. (frontiersin.org)
• Bone marrow examination showed the proliferation of hematopoietic cells were active, mainly granulocytes, and immature granulocytes increased. (frontiersin.org)
• The expression correlates with the proliferation state of the cells. (sigmaaldrich.com)
• Myc therefore is an important positive regulator of cell growth and proliferation. (sigmaaldrich.com)
• The most well characterized regulatory steps of adult hippocampal neurogenesis are the rate of progenitor cell proliferation and the survival rate of new DGCs. (jneurosci.org)
• The encoded protein binds directly to retinoblastoma protein, which regulates cell proliferation. (fishersci.com)
• Promotes cell proliferation. (thermofisher.com)
• Загальні відомості про плазмоклітинні захворювання Plasma cell disorders are a diverse group of disorders of unknown etiology characterized by Disproportionate proliferation of a single clone of B cells Presence of a structurally and electrophoretically. (msdmanuals.com)

Protein40

• However, in an investigation of BRAF localisation using fluorescence microscopy combined with subcellular fractionation of Green Fluorescent Protein (GFP)-tagged proteins expressed in NIH3T3 cells, surprisingly, we detected N-terminally truncated BRAF (ΔBRAF) in both nuclear and cytoplasmic compartments. (bath.ac.uk)
• However, no correlation was found between these cells and RA patient age, disease duration, or the levels of erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), rheumatoid factor (RF) and anti-citrullinated peptide antibody (ACPA). (bvsalud.org)
• D) Typical nuclear expression of a protein related to Kaposi's sarcoma-associated herpesvirus LANA in neoplastic cells. (cdc.gov)
• Deep RNA-DNA, RNA-protein interaction studies, and phenotype rescue analyses reveal that LETR1 is a nuclear trans-acting lncRNA modulating, via key epigenetic factors, the expression of essential target genes, including KLF4 and SEMA3C , governing the growth and migratory ability of LECs. (nature.com)
• Concordant results for the detection of TNFα at protein and mRNA levels were obtained with an unexpectedly low number of TNFα producing cells compared with IL1 expressing cells in many patients with RA. (bmj.com)
• Expression of the fusion protein cloned in pCLIP-H2B can be achieved by transiently transfecting cells in culture with standard transfection protocols. (neb.com)
• 2003), Darmin is a novel secreted protein expressed du. (xenbase.org)
• Darmin is a novel secreted protein expressed during endoderm development in Xenopus. (xenbase.org)
• I am a plant cell biologist and protein biochemist at Oxford Brookes University with expertise in the structure and function of the plant endoplasmic reticulum (ER), membrane proteins and auxin biosynthesis using biochemical techniques as well as high-resolution live cell imaging. (brookes.ac.uk)
• I am committed to interdisciplinary research, and an example of the successes gained from this approach is the project with Prof A Nabok (Engineering Sheffield Hallam University) using total internal reflection ellipsometry to quantify protein-membrane interactions on native plant membranes and human cell lines. (brookes.ac.uk)
• Program access grant to the STFC Harwell Laser Facility 'The Plant Cell Initiative: Protein interactions in the higher plant secretory pathway' 2017-2021 (approximate value of £200K). (brookes.ac.uk)
• The RNA-binding protein NANOS3 poses as an interesting candidate gene for POI since members of the Nanos family have an evolutionarily conserved function in germ cell development and maintenance by repressing apoptosis. (hindawi.com)
• The central effector of canonical Wnt signaling, β-catenin (CTNNB1), is a multifunctional protein, and has been extensively studied with respect to its roles in cell-cell adhesion and in regulation of Wnt-driven transcription. (nih.gov)
• Here, a novel mass spectrometry-based proteomics technique in colorectal cancer cells expressing stabilized β-catenin, was used to identify a protein-protein interaction between β-catenin and DNA methyltransferase I (Dnmt1) protein, the primary regulator of DNA methylation patterns in mammalian cells. (nih.gov)
• Dnmt1 and β-catenin strongly colocalized in the nuclei of colorectal cancer cells, and the interaction is mediated by the central domain of the Dnmt1 protein. (nih.gov)
• Dnmt1 protein abundance is dependent upon the levels of β-catenin, and is increased in cells expressing stabilized mutant β-catenin. (nih.gov)
• In summary, a functional protein-protein interaction was identified between two critically important oncoproteins, in turn revealing a link between Wnt signaling and downstream nuclear functions mediated by Dnmt1. (nih.gov)
• Laboratory tests showed leukocytosis (20.79 × 10 9 /L), moderate anemia (69 g/L), increased percent of neutrophils (91.6%), increased C-reactive protein (CRP) (80.90 mg/L), procalcitonin (PCT) (5.87 ng/ml) and erythrocyte sedimentation rate (ESR) (106.0 mm/h) level, and mild hypoalbuminemia (31.0 g/L). Stool routine revealed Leukocyte ++++/HP, pus cell ++++/HP, stool blood was positive. (frontiersin.org)
• Myc encodes a protein of 65 kDa which is expressed in almost all normal and transformed cells. (sigmaaldrich.com)
• I plan to express a gfp fusion protein where a nuclear protein is fused to the Nterm of gfp. (bio.net)
• This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. (fishersci.com)
• and a second, previously identified and constitutively expressed and non-oxygen-regulated protein known as ARNT. (nobelprize.org)
• In heterotrimeric G-protein signalling, cell surface receptors (GPCRs) are coupled to membrane-associated heterotrimers comprising a GTP-hydrolysing subunit G-alpha and a G-beta/G-gamma dimer. (embl-heidelberg.de)
• Mammalian Purkinje-cell protein-2 (Pcp2). (embl-heidelberg.de)
• It may function as a cell-type specific modulator for G protein-mediated cell signalling. (embl-heidelberg.de)
• A GTPase activator for the nuclear ras-related regulatory protein RAP-1A. (embl-heidelberg.de)
• Interaction of heterotrimeric G protein Galphao with Purkinje cell protein-2. (embl-heidelberg.de)
• The heterotrimeric G protein Galphao is ubiquitously expressed throughout the central nervous system, but many of its functions remain to be defined. (embl-heidelberg.de)
• Pcp2 (Purkinje cell protein-2) was identified as a partner for Galphao in this system. (embl-heidelberg.de)
• They combined "in-cell" nuclear magnetic resonance (NMR) spectroscopy, a bioreactor system and cutting-edge computational algorithms to determine protein structures in crowded intracellular environments for the first time. (analytica-world.com)
• This has made it difficult to see what each protein inside the cells does in its natural environment, despite the obvious biomedical benefits of knowing e.g. how a particular protein reacts when cells are subjected to chemical stimuli, like pharmaceutical drugs. (analytica-world.com)
• To tackle this challenge, a team from Tokyo Metropolitan University led by Assistant Professor Teppei Ikeya and Professor Yutaka Ito applied nuclear magnetic resonance (NMR) spectroscopy measurements to specific proteins expressed inside sf9 cultured insect cells, a strain of cells originally derived from a type of moth larva widely used for protein production. (analytica-world.com)
• Thus, they combined a sparse sampling -based rapid NMR measurement scheme with state-of-the-art computational methods employing statistical techniques like Bayesian inference , methods tailored to elucidate protein structures efficiently based on a limited amount of structural information from in-cell NMR spectra with inherently low-sensitivity. (analytica-world.com)
• A nuclear protein and tumor suppressor that contains a C-terminal PHD ZINC FINGER. (bvsalud.org)
• It is expressed in different isoforms in various tissues and interacts with TUMOR SUPPRESSOR PROTEIN P53 to negatively regulate cell growth. (bvsalud.org)
• In animals receiving excitotoxic lesions of the striatum we detected an eightfold increase of green ¯uorescent protein (GFP)-expressing cells. (lu.se)
• Diagnosis typically requires demonstration of M-protein (sometimes present in urine and not serum but rarely absent entirely) and/or light-chain proteinuria, and excessive plasma cells in the bone marrow. (msdmanuals.com)
• In 15 to 20% of patients, plasma cells secrete only Bence Jones protein. (msdmanuals.com)
• EDMD1 is caused by mutations in the EMD gene on the X chromosome that codes for the nuclear envelope protein emerin. (medscape.com)
• New mutations have been found in the synaptic nuclear envelope protein 1 ( SYNE1 ) gene and in the synaptic nuclear envelope protein 2 ( SYNE2 ) gene in a few families, also termed Nesprin-1 and Nesprin-2, respectively. (medscape.com)

Nuclei13

• Using a group of little interfering RNAs (siRNAs) concentrating on CtsW, we demonstrate that knockdown of CtsW leads to a loss of IAV nucleoprotein deposition in the nuclei of contaminated cells at 3?h postinfection. (epf2013.org)
• For Purkinje cell output to reach the rest of the brain it must be conveyed by neurons of the DCN or vestibular nuclei. (jneurosci.org)
• Cerebellar input arrives from climbing and mossy fibers to stimulate neurons in the deep nuclei and the cortex, in which climbing fibers stimulate Purkinje cells directly and granule cells excited by mossy fibers stimulate Purkinje cells by way of parallel fibers. (jneurosci.org)
• DSRCT is composed of small cells with round hyperchromatic nuclei and a dense fibrous or spindle cell stroma. (medscape.com)
• TGF-β1 activated accumulation of phosphorylated SMAD2 (pSMAD2-C) at centrioles of motile cilia and at cell nuclei. (springer.com)
• This triggered an increase in paracellular permeability via cellular redistribution of claudin 3 (CLDN3) from TJs into cell nuclei followed by disruption of epithelial integrity and formation of epithelial lesions. (springer.com)
• In summary, we demonstrate a role of motile cilia in TGF-β1 sensing and showed that TGF-β1 disturbs TJ permeability of conductive airway epithelia by redistributing CLDN3 from TJs into cell nuclei. (springer.com)
• Some of the nuclei are enlarged and overlapping with partial nuclear clearing, but without any intranuclear inclusions. (cap.org)
• These proteins are expressed in the nuclei of activated T cells in a distinctly biphasic fashion, with p55 and p75 induction occurring within minutes whereas the induction of p50 and p85 occurs after several hours. (jci.org)
• In contrast, p50 and p55 are constitutively expressed in the nuclei of U937 and THP-1 monocytic cells. (jci.org)
• Immunostaining confirmed expression of NL-RSKs was confined to nuclei of HEK293, SVEC (endothelial cells) or neonatal rat cardiomyocytes. (bmj.com)
• BN are cells which have two similar-sized nuclei (almost the same size). (bvsalud.org)
• For this reason they are not included in the daughter cells' nuclei, thus remaining in the cytoplasm of interphase cells [5, 10, 16, 35]. (bvsalud.org)

Antigen5

• Identification of primary effusion lymphoma and immunohistochemical staining of primary effusion lymphoma cells with Kaposi's sarcoma-associated herpesvirus latent nuclear-associated antigen (LANA)-specific antibody. (cdc.gov)
• Since the pioneering use of 131 I in differentiated thyroid cancer in the 1940s, remarkable achievements in nuclear medicine endoradiotherapy have been demonstrated, mainly in the treatment of neuroendocrine neoplasms by using 177 Lu-labeled somatostatin analogs or in the treatment of advanced prostate cancer using prostate-specific membrane antigen-directed radionuclide therapy. (snmjournals.org)
• Tissue repair was assessed by proliferating cell nuclear antigen (PCNA) immunoreactivity and by expression of keratinocyte growth factor (KGF) mRNA by real-time PCR. (cdc.gov)
• Epithelial proliferating cell nuclear antigen staining was increased at 1 and 3 days post exposure and was similar in the two groups. (cdc.gov)
• Antibody- and T-cell-based approaches to targeting of B-cell maturation antigen have shown efficacy. (msdmanuals.com)

Tissue13

• Here, using the hemimetabolous insect Blattella germanica , we show that nuclear hormone receptors Seven-up-B (BgSvp-B) and Fushi tarazu-factor 1 (BgFTZ-F1) have essential roles in the tissue- and stage-specific activation of adult CA JH-biosynthetic activity. (nature.com)
• Inflammation-associated tissue injury may activate stem/progenitor cells, and mutagenic stimuli from inflammation can accumulate multiple mutations and epigenetic changes in stem/progenitor cells [ 3 , 10 ]. (hindawi.com)
• SFTs of the pleura are localized mesenchymal neoplasms composed of fibroblastlike cells believed to arise from the subpleural connective tissue. (medscape.com)
• Specimens acquired arthroscopically from areas with maximum signs of macroscopic inflammation showed an increased number of TNFα producing cells in pannus tissue compared with that occurring in synovial villi of a given joint. (bmj.com)
• 6-16 However, most previous studies based on immunohistochemical staining of intra-articular cytokine production in RA have been performed on acetone fixed tissue, a procedure which does not readily allow subsequent discrimination of cytokine producing cells and cytokine binding cells. (bmj.com)
• Antigens present on cell surface membranes and extracellularly in the tissue specimens are generally accurately shown after acetone fixation, while the intracellular morphology is poorly preserved. (bmj.com)
• Monocytes (MC) are bone marrow (BM) derived mononuclear phagocytes that play an important role in innate immune response and are the major immune cell population in chronic tissue inflammatory ( 1 , 2 ). (frontiersin.org)
• Similarly, there was interest in using the procedure to produce cloned tissue and organs for possible future transplantation in the nuclear donor and perhaps other tissue- compatible recipients. (who.int)
• These changes in gene expression alter cell metabolism, tissue re-modeling, and even organismal responses such as increases in heart rate and ventilation. (nobelprize.org)
• OBJECTIVE- Chronic activation of the nuclear factor-κB (NF-κB) in white adipose tissue leads to increased production of pro-inflammatory cytokines, which are involved in the development of insulin resistance. (diabetesjournals.org)
• Multiple myeloma is a cancer of plasma cells that produce monoclonal immunoglobulin and invade and destroy adjacent bone tissue. (msdmanuals.com)
• [ 1 ] Even though these proteins are ubiquitously expressed, disease manifestations are tissue specific for as yet unclear reasons. (medscape.com)
• The first suggests that disruption of the inner nuclear membrane and the nuclear lamina causes disorganization of nuclear chromatin and gene expression, while the second proposes that the mechanical strength of the cell nucleus is disrupted when the nuclear lamina is weakened leading to structural and signaling defects in mechanically stressed tissue such as muscle and heart. (medscape.com)

Endothelial cells5

• We determine a comprehensive map of lineage-specific lncRNAs in human dermal lymphatic and blood vascular endothelial cells (LECs and BECs), combining RNA-Seq and CAGE-Seq. (nature.com)
• Thereafter, a distinct subpopulation of endothelial cells lining the cardinal vein starts differentiating by expressing the TF PROX1, the master regulator of lymphatic endothelial cell (LEC) identity, via the TFs SOX18 and COUPTFII. (nature.com)
• IL1α was predominantly seen in vascular endothelial cells, whereas IL1β staining was mainly shown in macrophages and fibroblasts. (bmj.com)
• Choroidal endothelial cells (RF/6A) were maintained in a semiconfluent state and hypoxia was induced by exposing the cells to cobalt chloride for 24 h. (molvis.org)
• However, its role has not been evaluated in hypoxic choroidal endothelial cells (CECs), the precursor of VEGF-mediated choroidal angiogenesis. (molvis.org)

Constitutively expressed1

• KGF mRNA was constitutively expressed in both groups and did not increase significantly after Cl2 but expression was lower in TCR-delta-/- mice. (cdc.gov)

Tissues12

• The expression of these Src family members are not the same throughout all tissues and cell types. (wikipedia.org)
• To investigate the involvement of stem cells in Epstein-Barr virus infection-related nasopharyngeal carcinoma (NPC), we used double immunofluorescence staining to examine several cancer stem/progenitor cell markers (CD44v6, CD24, and ALDH1A1) in NPC tissues and NPC cell lines. (hindawi.com)
• The staining intensity of 8-nitroguanine was significantly higher in cancer cells and inflammatory cells in the stroma of NPC tissues than in chronic nasopharyngitis tissues. (hindawi.com)
• Expression levels of CD44v6 and ALDH1A1 were significantly increased in cancer cells of primary NPC specimens in comparison to chronic nasopharyngitis tissues. (hindawi.com)
• 8-Nitroguanine was detected in both CD44v6- and ALDH1A1-positive stem cells in NPC tissues. (hindawi.com)
• Although the LAT family plays important roles in the development and function of normal tissues, they are frequently increased in cancer cells ( 3 ). (spandidos-publications.com)
• Mechanochemical Principles of Spatial and Temporal Patterns in Cells and Tissues. (mpi-cbg.de)
• Patterns are ubiquitous in living systems and underlie the dynamic organization of cells, tissues, and embryos. (mpi-cbg.de)
• No ethical problems were envisaged with the use of somatic cell nuclear transfer techniques which would lead directly to cloned differentiated cells or tissues such as skin, for future use by the nuclear donor. (who.int)
• It was found that the lncRNA ABHD11-AS1 expression levels are up-regulated in chronic Cr(VI) exposure-transformed human bronchial epithelial cells, chronically Cr(VI)-exposed mouse lung tissues, and human lung cancer cells as well. (cdc.gov)
• Bioinformatics analysis revealed that ABHD11-AS1 levels are up-regulated in lung adenocarcinomas (LUADs) tissues and associated with worse overall survival of LUAD patients but not in lung squamous cell carcinomas. (cdc.gov)
• genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)

Tumor7

• The role of tumor necrosis factor α (TNF-α) and interleukin 1 ß ( IL-1 ß) in inducing RANKL expression in B10 cells was evaluated by in vitro stimulation assay. (bvsalud.org)
• In conclusion, CD44v6 and ALDH1A1 are candidate stem cell markers for NPC, and the increased formation of DNA lesions by inflammation may result in the mutation of stem cells, leading to tumor development in NPC. (hindawi.com)
• These cells are defined as tumor-initiating cells or rare cells with indefinite potential for self-renewal that drives tumorigenesis [ 15 ]. (hindawi.com)
• Nuclear localized HER family receptor tyrosine kinases (RTKs) have been observed in primary tumor specimens and cancer cell lines for nearly two decades. (plos.org)
• Tumor necrosis factor alpha induces the nuclear expression of p55 and p75 in these monocytic cells within 20 min, presumably reflecting the liberation of these proteins from I kappa B. In contrast, phorbol myristate acetate (PMA) induces the expression of these proteins with delayed kinetics, raising the possibility that PMA is incapable of mediating the efficient release of p55 and p75 from I kappa B in these cells. (jci.org)
• William Kaelin, Jr. was in 1995 engaged in the study of the von Hippel-Lindau tumor suppressor gene, and after isolation of the first full-length clone of the gene showed that it could suppress tumor growth in VHL mutant tumorigenic cell lines. (nobelprize.org)
• Se expresa en diferentes isoformas en diversos tejidos e interacciona con la PROTEÍNA SUPRESORA DE TUMOR P53 para regular negativamente el crecimiento celular. (bvsalud.org)

Receptor10

• Increased receptor activator of nuclear factor kappa B ligand expressed on B10 cells in rheumatoid arthritis]. (bvsalud.org)
• To detect receptor activator of nuclear factor kappa B ligand (RANKL) expressed on B10 cells in rheumatoid arthritis (RA) and to evaluate the correlation between RANKL-producing B10 cells in RA and clinical features and laboratory parameters , trying to reveal the possible role of B10 cells in the pathogenesis of RA and the potential mechanism of impaired immunosuppressive capacities. (bvsalud.org)
• Since epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors, including gefitinib (GEF) have been reported to induce the apoptosis of several cancer cell lines, in the present study, we examined whether the cytotoxic effects of GEF are further enhanced under amino acid starvation (AAS) culture conditions. (spandidos-publications.com)
• Brand TM, Iida M, Luthar N, Wleklinski MJ, Starr MM, Wheeler DL (2013) Mapping C-Terminal Transactivation Domains of the Nuclear HER Family Receptor Tyrosine Kinase HER3. (plos.org)
• Classically, HER family members function from the cell surface, where binding to cognate ligands can induce receptor homo- or hetero-dimerization with other HER family receptors [3] . (plos.org)
• Ly6C high MC exhibited activated neutrophil degranulation, lysosome, cytokine production/receptor interaction and myeloid cell activation pathways, and Ly6C low MC presented features of lymphocyte immunity pathways in both mice. (frontiersin.org)
• Gastrin releasing peptide receptor (GRPR) may be a valid target for nuclear imaging of PCa. (rug.nl)
• The aim of this thesis was to develop novel bombesin tracers to target Gastrin Releasing Peptide Receptor (GRPR), which is over-expressed in Prostate Cancer (PCa). (rug.nl)
• This expanding panel of cell-based receptor assays, which currently includes 54 targets, is being offered by CMT for custom compound profiling to evaluate compound selectivity over a wide range of likely GPCR targets. (genengnews.com)
• GH receptor is expressed in human oocytes and cumulus cells, [ 12 , 13 ] and GH has been shown to promote in vitro nuclear maturation of denuded human oocytes. (medscape.com)

Apoptosis3

• The identification of an inactivating missense mutation in NANOS3 suggests a mechanism for POI involving increased primordial germ cells (PGCs) apoptosis during embryonic cell migration and highlights the importance of NANOS proteins in human ovarian biology. (hindawi.com)
• Myc has been demonstrated also to be a potent inducer of apoptosis when expressed in the absence of serum or growth factors. (sigmaaldrich.com)
• However,these neurons subsequently underwent cell death through apoptosis. (biologists.com)

Kappa4

• By immunostains, the lymphocytes are predominantly T cells, and plasma cells are polyclonal, expressing both kappa and lambda light chains. (cap.org)
• Nuclear expression of the 50- and 65-kD Rel-related subunits of nuclear factor-kappa B is differentially regulated in human monocytic cells. (jci.org)
• The nuclear factor (NF)-kappa B transcription factor system is composed of at least four inducible nucleoprotein adducts termed p50, p55 (NF-kappa B p50), p75 (NF-kappa B p65), and p85 (c-Rel). (jci.org)
• These findings highlight important differences in the regulation of these proteins in monocytic cells versus T cells and suggest that the induced expression of NF-kappa B p65 in monocytes may play a central role in the activation of HIV-1 gene expression. (jci.org)

Embryonic2

• It plays a role in the regulation of embryonic development and cell growth. (wikipedia.org)
• This proto-oncogene may play a role in the regulation of embryonic development and cell growth. (wikipedia.org)

Epithelial cells3

• Herein we elaborated effects of TGF-β1 on TJs of primary human bronchial epithelial cells. (springer.com)
• Lung epithelial cells form a barrier that lines airway and alveolar surfaces and constitute an air-liquid interface. (springer.com)
• c ) Current shape representations of 3D epithelial cells: prism, frustum, prismatoid, and scutoid. (elifesciences.org)

Gene12

• This discovery changed the current thinking about cancer from a model wherein cancer is caused by a foreign substance (a viral gene) to one where a gene that is normally present in the cell can cause cancer. (wikipedia.org)
• Our findings demonstrate that acquisition of MEK inhibitor resistance often occurs through gene amplification and can be suppressed by impeding cell cycle entry in drug. (babraham.ac.uk)
• cndp2 (CNDP dipeptidase 2 (metallopeptidase M20 family)) gene expression in Xenopus laevis embryo , assayed via in situ hybridization, NF stage 3 (4-cell), dorsal view. (xenbase.org)
• Members of the evolutionarily conserved Nanos gene family are preferentially expressed in the ovaries and are known to play an important role in germ cell development, maintenance, and survival [ 24 - 30 ]. (hindawi.com)
• Myc proto-oncogene encodes nuclear DNA-binding phosphoproteins that are involved in the regulation of gene expression and DNA replication during cell growth and differentiation. (sigmaaldrich.com)
• Lesser ERK1/2 activation by PE causes nuclear activation of only RSK2, and this signalling is associated with a different gene expression profile. (bmj.com)
• Our hypothesis is that activation of nuclear-localised RSK1 in addition to RSK2 promotes changes in gene expression that are not compatible with compensated hypertrophy induced by α1ARs. (bmj.com)
• Animal cells undergo fundamental shifts in gene expression when there are changes in the oxygen levels around them. (nobelprize.org)
• Through the combined work of these three laureates it was thus demonstrated that the response by gene expression to changes in oxygen is directly coupled to oxygen levels in the animal cell, allowing immediate cellular responses to occur to oxygenation through the action of the HIF transcription factor. (nobelprize.org)
• Pcp2 was first identified as a candidate gene to explain Purkinje cell degeneration in pcd mice (Nordquist, D. T., Kozak, C. A., and Orr, H. T. (1988) J. Neurosci. (embl-heidelberg.de)
• The vast majority of these cells did not divide, suggesting that the transgene was indeed regulated in a similar fashion as the endogenous GFAP gene. (lu.se)
• Emerin is a ubiquitous inner nuclear membraneprotein, presentin nearly all cell types, although its highest expression is in skeletal and cardiacmuscle.Emerin binds to many nuclear proteins, including several gene-regulatory proteins (eg, barrier-to-autointegration factor, germ cell-less, Btf), nesprins (proteins that act as molecular scaffolds), F-actin, and lamins. (medscape.com)

Assays9

• To understand how the B 1 and B 2 regions influenced the transcriptional functions of nuclear HER3, we performed cyclin D1 promoter-luciferase assays in which HER3 deleted of the B 1 and B 2 regions was severely hindered in regulating this promoter. (plos.org)
• Cell-based and in vitro assays show that Retro-2 blocks delivery of newly-synthesized TA-proteins to the ER-targeting factor ASNA1 (TRC40). (elifesciences.org)
• Screening using cell-based assays has been performed for many years, but recent technologies allow screening of cells that are alive and changing. (genengnews.com)
• Cell-based assays are becoming increasingly popular for small molecule discovery in high-throughput screening. (genengnews.com)
• As cell-based assays are used more commonly in robotic high-throughput compound screening, cells themselves have become critical reagents. (genengnews.com)
• We experimented with cells division-arrested with low level mitomycin C treatment and demonstrated that they perform with better consistency than non-division arrested counterparts in high-content screening imaging assays, says Zhong Zhong, vp of development at CMT. (genengnews.com)
• We developed Redistribution cell-based assays for use in both primary screening and lead optimization of compounds targeting GPCRs. (genengnews.com)
• Redistribution assays are independent of the imaging platform and have been shown to give consistent results on a number of instruments on the market, including: IN Cell 3000 (GE Healthcare), Opera (Evotec Technologies), ArrayScan (Cellomics), Pathway HT (BD Biosciences), Discovery1 (Molecular Devices), and Icyte (CompuCyte). (genengnews.com)
• Redistribution assays can be multiplexed by either expressing two targets in the same cell line labeled with different colors, or by mixing different assay cell lines directly in the microtiter plate well. (genengnews.com)

Vectors3

• Methods/results Nuclear-localised (NL) RSK1 or RSK2 were expressed in cultured cells using adenoviral vectors. (bmj.com)
• There are a number of hypothesized that regulating the transgene with a GFAP promoter in a different vectors that transduce cells in the brain in a slightly different viral vector would give rise to a high transgenic expression in the manner (for a review see, e.g. (lu.se)
• These vectors have a number of appealing features including the expression by using the machinery of the host cell instead of depending abilities to ef®ciently transduce cells in the central nervous system, on recombinant regulatory proteins. (lu.se)

Nucleus2

• Nuclear GFP-ΔBRAF retains MEK/ERK activating potential and is associated with the accumulation of phosphorylated MEK and ERK in the nucleus. (bath.ac.uk)
• Hürthle cell neoplasms of the thyroid show a distinct nodule or nodules that are comprised mostly of large oncocytes with distinct cell borders, deeply eosinophilic and granular cytoplasm, and a large nucleus with prominent nucleolus. (cap.org)

Differentiation1

• Ganglion cells, cone photoreceptors, horizontal cells and amacrine cells were produced and initiated differentiation. (biologists.com)

Progenitor1

• Adult neurogenesis is a complex process whereby neural progenitor cells adopt a neuronal fate, migrate, and mature over several weeks. (jneurosci.org)

A5495

• A) A549 cells had been transfected with siRNAs and contaminated with A/Ukraine/1/1963 (MOI of 10) at 48?h posttransfection. (epf2013.org)
• Demeclocycline HCl (B) A549 cells had been transfected with siRNAs and contaminated with lymphocytic choriomeningitis trojan (LCMV) at 48?h posttransfection. (epf2013.org)
• C) A549 control cells or CtsW-overexpressing cells had been contaminated with A/WSN/33 (MOI of 50) for 60 min on glaciers and then used in 37C for 60 or 120 min before these were lysed. (epf2013.org)
• A549 control cells, A549 stably expressing CtsW_res #2 or CtsW(C153A)_res #2 had been contaminated with A/WSN/33 at an MOI of 0.01. (epf2013.org)
• Under AAS culture conditions, the cell killing effect of GEF was synergistically pronounced in the EGFR-expressing cell lines, namely, CAL 27, Detroit 562, A549 and PANC-1 cells compared with those treated with either GEF or AAS alone. (spandidos-publications.com)

Cytoplasmic1

• The analysis shows strong cytoplasmic and nuclear positivity in neuronal cells. (thermofisher.com)

Drosophila3

• Fluorescent reporter proteins in the Drosophila model system offer a degree of specificity that allows monitoring cellular and biochemical phenomena in vivo, such as autophagy, mitophagy, and changes in the redox state of cells. (intechopen.com)
• 3D super-resolution imaging of the nuclear envelope of a Drosophila cell. (cnrs.fr)
• Nanos was first identified in Drosophila , where it represses the translation of target mRNAs through binding to their 3′ UTR and has a conserved function in germ cell development across species. (hindawi.com)

Antibody1

• and 177 Lu-lilotomab, a CD37 antibody for the treatment of B-cell lymphomas, are being highlighted. (snmjournals.org)

Transcription5

• During development, the blood vascular system arises from endothelial cell progenitors that differentiate from mesodermal cells, mostly through the expression of the transcription factor (TF) ETV2. (nature.com)
• Conversely, the Dnmt1 regulates the levels of nuclear β-catenin and β-catenin/TCF-driven transcription. (nih.gov)
• Significantly differentially expressed (SDE) immunological genes and transcription factor (TF) were selected for functional pathways and transcriptional signaling identification. (frontiersin.org)
• Transcription is repressed in quiescent or terminally differentiated cells. (sigmaaldrich.com)
• We use RT-PCR reverse transcription system and the expand high fidelity PCR system to detect spliced XBP1 mRNA, and fraction cells to determine the location of the unconventional splicing of XBP1 mRNA. (mdpi.com)

Cellular1

• However, cellular activation is required for the nuclear expression of p75 in these cells. (jci.org)

Genes5

• High-throughput screen for genes predominantly expressed in the ICM of mouse blastocysts by whole mount in situ hybridization. (nih.gov)
• The genes encoding the enzymes involved in both modules are highly enriched, or even exclusively expressed, in the CA cells 9 . (nature.com)
• Approximately 20 genes consistently associated with T2DM mainly implicate pancreatic β-cell function in the pathogenesis of T2DM. (scialert.net)
• Selumetinib causes long-term G1 arrest accompanied by reduced expression of DNA replication and repair genes, but cells stochastically re-enter the cell cycle during treatment despite continued repression of pERK1/2. (babraham.ac.uk)
• Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)

Expression9

• After in vitro stimulation by TNF-α, but not IL-1 ß, B10 cells isolated from healthy donors demonstrated fundamentally upregulated expression of RANKL. (bvsalud.org)
• Activation of the VEGFA/VEGFR2 signaling and expression of blood vascular endothelial cell (BEC) markers, such as NRP1 and EphrinB2, further differentiate these precursor cells into BECs, which then form the hierarchical network of blood vessels 4 . (nature.com)
• It is possible to use pools of stable cell populations for initial cell labeling to test for the presence of CLIP-tag expression. (neb.com)
• Darmin-r was maternally expressed and showed at later stages expression in the lens and pronephric glomus . (xenbase.org)
• Human MC were initially divided into three subsets based on the cell surface expression of CD14 and CD16, and recently classified based on CD40 expression ( 2 - 5 ). (frontiersin.org)
• Expression of Myc is generally induced after mitogenic stimulation of cells or serum induction. (sigmaaldrich.com)
• Our aim was to investigate if expression of nuclear-localised RSK1 is detrimental to the hypertrophic response induced in vivo by PE. (bmj.com)
• It was further determined that up-regulation of ABHD11-AS1 expression plays an important role in chronic Cr(VI) exposure-induced cell malignant transformation and tumorigenesis, and the stemness of human lung cancer cells. (cdc.gov)
• 2000). We of glial cell line-derived neurotrophic factor (GDNF) in models of have recently reported that this vector directs transgene expression to Parkinson's disease (Georgievska et al. (lu.se)

Highly expressed3

• Both factors are highly expressed in adult CA cells. (nature.com)
• Ly6C high MC expressed higher levels of macrophage (MΦ) markers, whereas, Ly6C low MC highly expressed lymphocyte markers in both mice. (frontiersin.org)
• GRPR is highly expressed in several human cancer cell lines and could be used as a potential target for cancer detection. (rug.nl)

Ubiquitously1

• Src, Fyn and Yes are expressed ubiquitously in all cell types while the others are generally found in hematopoietic cells. (wikipedia.org)

Mice7

• Previously we reported that increased lateral deviation while ambulating and slips while traversing a narrow beam of ataxic Kcnc3 -null mice were corrected by restoration of Kv3.3 channels specifically to Purkinje cells, whereas Kcnc3 -mutant mice additionally lacking one Kcnc1 allele were partially rescued. (jneurosci.org)
• As Kcnc1 , but not Kcnc3 , alleles are lost, mutant mice exhibit increasing gait ataxia accompanied by spike broadening and deceleration in DCN neurons, suggesting the facet of coordination rescued by Purkinje-cell-restricted Kv3.3 restoration in mice lacking just Kcnc3 is hypermetria, while gait ataxia emerges when additionally Kcnc1 alleles are lost. (jneurosci.org)
• In mice, Nanos3 is expressed in the primordial germ cells (PGCs) from their formation until shortly after their appearance in the gonads (E13.5 in female and E14.5 in male embryos) [ 24 ]. (hindawi.com)
• I plan to express this in the mouse and FACS sort live cells obtained from such mice. (bio.net)
• The role of gamma delta T cells in airway epithelial injury and bronchial responsiveness after chlorine gas exposure in mice. (cdc.gov)
• Epithelial cell counts in BAL after Cl2 exposure were greater in TCR-delta-/- mice, but macrophages showed a later peak and granulocyte numbers were lower in TCR-delta-/- than in wild type mice. (cdc.gov)
• One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)

Replication1

• The results allowed comparison of replication timing between polytene chromosomes in salivary glands and chromosomes from cultured diploid cell lines and to observe a substantial similarity in the global replication patterns at the band resolution level. (sdbonline.org)

Pronounced cytotoxicity1

• The addition of essential amino acids, but not non-essential amino acids to the cell culture medium resulted in the cancellation of this pronounced cytotoxicity. (spandidos-publications.com)

Malignant2

• The term fibroma is inappropriate because the neoplasm exhibits defined histologic features that differ from those of fibromas and may express malignant behavior. (medscape.com)
• Together, these findings indicate that lncRNA ABHD11-AS1 interacts with SART3 and regulates CD44 RNA alternative splicing to promote cell malignant transformation and lung carcinogenesis. (cdc.gov)

Eukaryotic4

• Macroautophagy (thereafter designated as autophagy) is a self-digestive system conserved in all eukaryotic cells. (spandidos-publications.com)
• Eukaryotic cells destroy many endocytosed pathogens by targeting them to the lysosome. (elifesciences.org)
• Researchers from Tokyo Metropolitan University have successfully determined the high-resolution three-dimensional structure of proteins inside living eukaryotic cells. (analytica-world.com)
• Eukaryotic cells are the building blocks of a vast range of organisms, including all fungi, plants and animals. (analytica-world.com)

Translocation2

• Here we show that GFP-ΔBRAF nuclear translocation does not involve a canonical Nuclear Localisation Signal (NLS), but is suppressed by N-terminal sequences. (bath.ac.uk)
• Required for nuclear translocation of FLOT1. (thermofisher.com)

Stem cell transplant1

• High-dose melphalan followed by autologous peripheral blood stem cell transplantation may also be used. (msdmanuals.com)

Radioactive3

• the rate of decay of radioactive material expressed as the number of atoms breaking down per second measured in units called becquerels or curies . (cdc.gov)
• Man-made sources of radioactive materials are found in consumer products, industrial equipment, atom bomb fallout, and to a smaller extent from hospital waste, medical devices, and nuclear reactors. (cdc.gov)
• Radioactive forms of cesium are produced by the fission of uranium in fuel elements (fuel rods) during the normal operation of nuclear power plants, or when nuclear weapons are exploded. (cdc.gov)

Chromatin1

• Electron microscopy demonstrated that the CAL 27 cells treated with GEF under AAS culture conditions exhibited swelling of the cytosol and organelles with an increased number of autophagosomes and autolysosomes, but without chromatin condensation and nuclear fragmentation. (spandidos-publications.com)

Metabolism1

• Growth hormone (GH) is a peptide hormone secreted by the anterior pituitary gland in pulsatile manner, and it has important roles in cell growth and metabolism throughout the body. (medscape.com)

Embryos1

• Several participants reported interest among the scientific and medical communities of their countries and regions in the use of somatic cell nuclear transfer techniques to produce cloned human embryos for time-limited basic research on ageing and genetic diseases. (who.int)

Interacts1

• Nuclear localized USP15 interacts with pre-mRNA processing factor 19 (PRPF19) to increase CD44 RNA alternative splicing activating β-catenin and enhancing cancer stemness. (cdc.gov)

Lines4

• Together, our study provides several lines of evidence supporting the intriguing concept that every cell type expresses precise lncRNA signatures to control lineage-specific regulatory programs. (nature.com)
• In addition clonal cell lines can be isolated and characterized if desired. (neb.com)
• c ) Normalised apical and basal cell area distributions in the tip (broken lines) and tube (solid lines) datasets. (elifesciences.org)
• The advantage of our division-arrested cell lines is that it is a live cell, yet it is not dividing, Zhong explains. (genengnews.com)

Promotes2

• Conclusions Increased nuclear-localised RSK1 in cardiomyocytes promotes cardiac hypertrophy, but compromises the compensated hypertrophic response to PE. (bmj.com)
• The interaction of ABHD11-AS1 with SART3 promotes USP15 (ubiquitin specific peptidase 15) nuclear localization. (cdc.gov)

Assay3

• www.cmt-inc.net), makes it possible to use frozen assay-ready cells for screening as if they were ordinary assay reagents like enzymes or membrane preparations. (genengnews.com)
• The idea of a robust, cell-based assay is no longer an oxymoron. (genengnews.com)
• CMT also offers a cell-based assay technology based on Sentigen Biosciences (www.sentigen.com) Tango Assay System. (genengnews.com)

Neurons7

• Thus, fast repolarization in Purkinje cells appears important for normal movement velocity, whereas DCN neurons are a prime candidate locus where fast repolarization is necessary for normal gait patterning. (jneurosci.org)
• Purkinje cells in turn inhibit deep nuclear neurons also, at a latency, leading to rebound excitation. (jneurosci.org)
• Large, non-GABAergic DCN projection neurons express Kv3.1 and Kv3.3 channel subunits. (jneurosci.org)
• Finally, neurons developing within a highly active environment exhibited a delayed maturation when their intrinsic electrical activity was reduced by the cell-autonomous overexpression of Kir2.1, an inward-rectifying potassium channel. (jneurosci.org)
• By contrast, Sfrs1 was not required for the survival of the neurons generated later, including later-born amacrine cells, rod photoreceptors,bipolar cells and Müller glia. (biologists.com)
• It is uniquely expressed in cerebellar Purkinje cells and in retinal bipolar neurons. (embl-heidelberg.de)
• Pcp2 is expressed in cerebellar Purkinje cells and retinal bipolar neurons, two locations where Galphao is also expressed. (embl-heidelberg.de)

Markers1

• Endoderm development is an area of intense interest in developmental biology, but progress has been hampered by the lack of specific markers for differentiated endodermal cells. (xenbase.org)

Regulation2

• and regulation of cell motility. (nih.gov)
• Next, we identified full-length nuclear HER3 association and regulation of a 122 bp region of the cyclin D1 promoter. (plos.org)

Lymphoma9

• A) Diffuse infiltration of the pleura pulmonalis and pleural space with pleomorphic round cells resembling primary effusion lymphoma. (cdc.gov)
• Extranodal NK/T cell lymphoma is a rare non-Hodgkin lymphoma mainly involving the upper aerodigestive tract, even rarer is primary extranasal disease involving the intestine. (frontiersin.org)
• We present a case of primary intestinal NK/T cell lymphoma with diagnostic challenge, which eventually developed into multiple intestinal perforations. (frontiersin.org)
• The surgical samples underwent pathological analysis, and a diagnosis of extranodal NK/T cell lymphoma nasal type was confirmed. (frontiersin.org)
• Early diagnosis of primary intestinal NK/T cell lymphoma is frequently difficult. (frontiersin.org)
• Extranodal NK/T cell lymphoma nasal type (ENKTL) is a subtype of non-Hodgkin lymphoma (NHL). (frontiersin.org)
• It is an invasive lymphoma derived from NK cells or cytotoxic T cells, which is related to Epstein-Barr virus infection. (frontiersin.org)
• Diffuse large B-cell lymphoma is the most common histologic subtype of NHL, with ENKTL of the large bowel being less frequent ( 4 ). (frontiersin.org)
• Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)

Patterns1

• These neoplasms can show follicular, trabecular, solid, or papillary growth patterns with occasional nuclear grooves or nuclear pseudoinclusions. (cap.org)

Carcinoma1

• The distinction between Hürthle cell adenoma and carcinoma is based on presence of capsular or vascular invasion, or both. (cap.org)

Mutations3

• Moreover, several studies have shown that cancer cells have genetic instability, epigenetic changes, and an accumulation of mutations, suggesting that cancer is a genetic disease [ 16 ]. (hindawi.com)
• Melanoma in particular exhibits a high incidence of activating BRAF and NRAS mutations and such cells are addicted to the activity of these mutant oncoproteins. (babraham.ac.uk)
• Whatever the true mechanism, the discovery of mutations in several different nuclear membrane proteins that cause similar diseases will likely eventually lead to a better understanding of nuclear membrane physiology and the pathophysiology of diseases caused by mutations in these proteins. (medscape.com)

Fluorescence2

• Note that the intensity of the fluorescence may vary depending on cell line and labeling substrate used. (neb.com)
• Labeling of transfected cells with a fluorescent CLIP-Cell substrate should show strong nuclear fluorescence. (neb.com)

High-throughput1

• Advanced high-throughput screening technologies require standardized, consistent cell culture as reagents to generate reliable results. (genengnews.com)

Roles2

• During inflammation, reactive oxygen species (ROS) and reactive nitrogen species (RNS) are generated from inflammatory cells and are considered to play key roles in carcinogenesis [ 3 ]. (hindawi.com)
• Lamin A/C proteins have key roles in nuclear structural integrity and chromosomal stability. (mdpi.com)

Significantly1

• The problem with simply applying the same techniques to proteins in sf9 cells was the significantly lower concentration of target proteins and short lifetime of cells, making it difficult to collect high quality multi-dimensional NMR spectra for nuclear Overhauser effect spectroscopy (NOESY) which would give precise information about how different atoms are spaced inside individual molecules. (analytica-world.com)