Cells ex vivo. Medical search. Frequent questions

The funding from the Gates Foundation will advance Intellia's preclinical validation of in vivo hematopoietic stem cells (HSCs) genome editing using the company's proprietary non-viral delivery systems and CRISPR/Cas9 technology to potentially cure SCD. (newswise.com)
Hematopoietic stem cell transplantation (HSCT) involves the intravenous infusion of hematopoietic stem cells in order to reestablish blood cell production in patients whose bone marrow or immune system is damaged or defective. (medscape.com)

Mesenchymal stem cells (MSCs) possess great therapeutic potential. (hindawi.com)
The differentiation of mesenchymal stem cells (MSCs) into multiple cell lineages can be exploited as an attractive strategy for cell-based therapy and regenerative medicine [ 1 ]. (hindawi.com)
Pittenger, M.F. and Martin, B.J. (2004) Mesenchymal stem cells and their potential as cardiac therapeutics. (scirp.org)
Barry, F.P. and Murphy, J.M. (2004) Mesenchymal stem cells: Clinical applications and biological characterization. (scirp.org)
Caplan, A.I. and Bruder, S.P. (2001) Mesenchymal stem cells: Building blocks for molecular medicine in the 21st century. (scirp.org)
Huang, G.T., Gronthos, S. and Shi, S. (2009) Mesenchymal stem cells derived from dental tissues vs. those from other sources: Their biology and role in regenerative medicine. (scirp.org)
Key mesenchymal components (smooth muscle, intestinal subepithelial myofibroblasts and ganglion cells) were seen. (ca.gov)
Adipose tissue (AT) represents a commonly used source of mesenchymal stem/stromal cells (MSCs) whose proregenerative potential has been widely investigated in multiple clinical trials worldwide. (mdpi.com)
Sun J, Teng G, Ma Z, Ju S. In vivo monitoring of magnetically labeled mesenchymal stem cells intravascularly administered in rat acute renal failure. (smw.ch)
Mesenchymal stem cell medium was used in the control groups. (scielo.br)

Analysis of mitosis and antimitotic drug responses in tumors by in vivo microscopy and single-cell pharmacodynamics. (nature.com)
Tumors frequently induce immature myeloid cells (iMC), which suppress specific and unrelated cytotoxic T lymphocyte (CTL) responses and are termed myeloid derived suppressor cells (MDSC). (mdc-berlin.de)
A Novel Ex Vivo Method for Visualizing Live-Cell Calcium Response Behavior in Intact Human Tumors. (duke.edu)
By live in vivo imaging, ovarian cancer xenograft tumors were reduced after primary treatment with carboplatin. (duke.edu)

To better understand Plasmodium biology, researchers can whether proceed to in vitro studies or use in vivo models. (wjgnet.com)
In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)

show that teratoma formation can be minimized by transplanting the cells into an engineered niche that promotes efficient differentiation. (nature.com)
In this Chapter, we dissect the proof-of-principle studies that demonstrated that cellular reprogramming to pluripotency can be induced in vivo, in spite of unfavorable pro-differentiation signals present within the tissues. (springer.com)
Establishment of germ-line-competent embryonic stem (ES) cells using differentiation inhibiting activity. (springer.com)
In addition, their osteogenic differentiation potential was increased, and genes involved in cell adhesion, FGF-2 signalling, cell cycle, stemness, cell differentiation, and cell proliferation were upregulated, compared to that of the MSCs cultured on uncoated plates. (hindawi.com)
The ECM provides structural and biochemical support to the cells and has various other functions including cell adhesion, cell to cell communication, and differentiation [ 17 , 18 ]. (hindawi.com)
This study was designed to determine the in vivo performance of three different materials as scaffolds for dental pulp stem cells (DPSC) undergoing induced odontogenic differentiation. (scielo.br)
They have a higher odontogenic differentiation capacity compared to other cell groups due to their content of specific progenitor cells. (scielo.br)
The odontogenic differentiation of human dental pulp stem cells on nanofibrous poly(L-lactic acid) scaffolds in vitro and in vivo. (scielo.br)
Recent advances in live imaging and genetics of mammalian division, movement and cell differentiation leading to development which integrate observations of biochemical tissue formation [14 ]. (lu.se)

We show here, using a chimeric severe combined immunodeficiency (SCID) mouse (hu-SCID) model, that human Vγ2Vδ2 T cells mediate resistance to extracellular gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli and Morganella morganii) bacteria, as assessed by survival, body weight, bacterial loads, and histopathology. (jci.org)
Therefore, this large pool of antigen-specific, yet immediately reactive memory human Vγ2Vδ2 T cells is likely to be an important mediator of resistance against extracellular bacterial infection and may bridge the gap between innate and acquired immunity. (jci.org)
The extracellular matrix (ECM) provides structural and biochemical support to the surrounding cells, and it has been used as a coating substrate for cell culture. (hindawi.com)
MSCs naturally reside in a specialized niche in vivo , which mainly consists of the extracellular matrix (ECM). (hindawi.com)
In this study, we developed an EV production method utilizing in vivo -mimicking extracellular matrix-based 3D cultures, which allows tracking of EVs over the course of invasive development of tumor organoids. (biorxiv.org)
Here, we describe an approach that couples vibratome-mediated viable tissue sectioning with live-cell confocal microscopy imaging to visualize human parathyroid adenoma tumor cell responsiveness to extracellular calcium challenge. (duke.edu)
Live-cell observation of biochemical signaling in response to extracellular calcium challenge in the intact tissue slices reveals discrete, heterogeneous kinetic waveform categories of calcium agonist reactivity within each tumor. (duke.edu)

The findings demonstrate cell rescue by in vivo gene transfer, thus supporting the feasibility of treating an inherited retinal degeneration by somatic gene therapy. (nih.gov)
We demonstrate an in vivo electroporation protocol for transfecting single or small clusters of retinal ganglion cells (RGCs) and other retinal cell types in postnatal mice over a wide range of ages. (jove.com)

In parallel, the development of animal models opened new opportunities to study parasite biology in vivo . (wjgnet.com)
The International Journal of Biochemistry & Cell Biology, 36, 568-584. (scirp.org)

In vivo engraftment of stem cell-derived cells into damaged or diseased tissue has many challenges. (nature.com)
For one, if pluripotent stem cells are introduced in numbers large enough for tissue regeneration, there is a high risk of teratomas in an immunodeficient mouse (a standard research model for studying engraftment). (nature.com)
The links of in vivo reprogramming to pluripotency with tumorigenesis and teratoma formation, and the cross-talk with cellular senesce and tissue injury are also discussed. (springer.com)
Quantification of cell-cycle state at a single-cell level is essential to understand fundamental three-dimensional (3D) biological processes such as tissue development and cancer. (nature.com)
The effect of laminin on hard tissue formation using rat bone marrow cells was assessed. (scirp.org)
From the in vivo examination, laminin coating of the scaffolds induced hard tissue in the pores with the cells. (scirp.org)
Yoshikawa, M. , Kakigi, H. , Yabuuchi, T. and Hayashi, H. (2014) Effects of laminin on hard tissue formation by bone marrow cells in vivo and in vitro . (scirp.org)
Human tissue-engineered colon forms from postnatal progenitor cells: an in vivo murine model. (ca.gov)
ReNeuron Group plc (AIM: RENE), a UK based leader in stem cell derived exosome technologies, announces that Dr Samantha Thomas will be presenting in vivo data exemplifying the cellular and tissue targeting capabilities and highlighting the significant advantages of ReNeuron's CustomEX™ drug delivery platform, at Cell 2023 in London. (euroland.com)
Through its conditionally immortalised induced pluripotent stem cell (iPSC) platform, the Group can make allogeneic tissue cells of choice and has the potential to produce exosomes with tissue specific targeting ability. (euroland.com)
Hard tissue formation in a porous HA/TCP ceramic scaffold loaded with stromal cells derived from dental pulp and bone marrow. (scielo.br)
The lung alveolar epithelium represents a major site of tissue injury in IPF and senescence of this cell population is probably detrimental to lung repair. (ersjournals.com)
Here we demonstrate that lung epithelial cells exhibit increased P16 and P21 expression as well as senescence-associated β-galactosidase activity in experimental and human lung fibrosis tissue and primary cells. (ersjournals.com)
Surface associated glycoproteins of endothelial origin, or derived from pericytes, intravascular leukocytes, and plasma, are other important components of the glycocalyx, constituting a vascular cell surface proteome that is dynamic, tissue-specific, and sensitive to changes in vascular homeostasis, blood infection, and inflammation. (lu.se)
The functional impact of intratumoral heterogeneity has been difficult to assess in the absence of a means to interrogate dynamic, live-cell biochemical events in the native tissue context of a human tumor. (duke.edu)
Subsequent fixation and immunofluorescence analysis of the functionally evaluated tissue specimens allows alignment and mapping of the physical characteristics of individual cells within the tumor to specific calcium response behaviors. (duke.edu)
Evaluation of the relative abundance of intracellular PTH in tissue slices challenged with variable calcium concentrations demonstrates that production of the hormone can be dynamically manipulated ex vivo. (duke.edu)
The capability of visualizing live human tumor tissue behavior in response to experimentally controlled conditions opens a wide range of possibilities for personalized ex vivo therapeutic testing. (duke.edu)
Moreover, the homing capacity of the PEGA-pVEC and CREKA-pVEC was conserved in vivo, where the conjugates mainly accumulated in blood vessels in breast tumor tissue and were subsequently translocated into cells. (avhandlingar.se)
That more dendritic cells were required to stimulate rejection in reversed diabetic recipients compared with nondiabetic recipients suggests that other factors, such as the diabetic state and the production of a tolerant status achieved by larger amounts of grafted tissue, may influence graft survival. (diabetesjournals.org)
A histological examination of tissue sections, undertaken after the administration of AcAc LDL or Ac LDL (labeled with either 125I or a fluorescent probe) to rats, dogs, or guinea pigs, was used to identify the specific cells binding and internalizing these lipoproteins in vivo. (rupress.org)
the SH3BP2 protein is particularly important for the function of cells involved in the replacement of old bone tissue with new bone (bone remodeling) and certain immune system cells. (medlineplus.gov)
which are cells that break down bone tissue during bone remodeling. (medlineplus.gov)
of the most critical events which lead to tissue patterning involves mechanisms going beyond single cells. (lu.se)

The specific aims are: 1) Determine the efficacy and potency of anti-human GP88 monoclonal antibody AG1 in mono-therapy for non small cell carcinoma in nude mice xenograft models. (inknowvation.com)

Conclusion: Colon can form from human progenitor cells on a scaffold in a mouse host. (ca.gov)

Indeed, the recently developed 2-photon intravital microscopy technique (2P-IVM) allows capturing videos of cells while interacting in vivo, in organs of living organisms. (usi.ch)
In vivo imaging of ER with two-photon microscopy detected the ER enlargement. (tmd.ac.jp)
Uptake of AcAc LDL by endothelial cells of the liver, spleen, and bone marrow was confirmed by transmission electron microscopy. (rupress.org)

We will examine the role of proteins in achieving functional cell polarity compatible with efficient motility and response to directional cues, using unique techniques and analysis tools in the context of the living organism. (europa.eu)
Herein, we report morphologic, phenotypic and functional evaluation of Class II+/CD11c+ cells generated by in vivo administration of ProGP-4. (medscimonit.com)
CONCLUSIONS: The data presented in this report highlight the ability of E. coli derived ProGP-4 to expand large numbers of functional DC in the peripheral blood and lymphoid organs in vivo using a rodent model of hematopoiesis. (medscimonit.com)
Conventional histological methods can reveal morphology and static biomarker expression patterns but do not provide a means to probe and evaluate tumor functional behavior and live-cell responsiveness to experimentally controlled stimuli. (duke.edu)
In mutant HD-model mice, the ER enlarges, and the cell body forms an asymmetrical balloon shape until, finally, it ruptures.Pharmacological and genetic analyses revealed that this subtype of necrotic cell death is distinct from the RIP1/3 pathway-dependent necroptosis, as it is mediated by a functional deficiency of TEAD/YAP-dependent transcription. (tmd.ac.jp)
IkappaBalpha levels did not diminish even though the protein became phosphorylated and ubiquitinated, as determined based on detection of the characteristic high-molecular-weight form of the protein, and the 26S proteasome remained functional in VZV-infected cells. (simulations-plus.com)
The present review describes the unique properties and in vivo roles of the Rab27 system, and the functional relationship among different effectors coexpressed in single cells , with pancreatic beta cells used as an example.Key words membrane trafficking, regulated exocytosis , insulin granules, pancreatic beta cells . (bvsalud.org)

It combines image analysis and machine learning methods for automated 3D segmentation and cell-cycle state identification of individual cell-nuclei with widely varying morphologies embedded in complex tumor environments. (nature.com)
The precise function of the identified proteins will be determined by combining mathematical tools aimed at quantitatively gauging the role of the molecules in conferring proper cell shape, biophysical methods aimed at measuring forces, rigidity and cytoplasm flow and determination of the effect on the organization of relevant structures using cryo electron tomography. (europa.eu)
Hence, computer vision methods have the potential to extract biomedical knowledge from these videos by analyzing the movement of cells. (usi.ch)
Unfortunately, common methods for movement analysis poorly apply to 2P-IVM videos of leukocytes (the main cells of the immune system). (usi.ch)

The ability to label and genetically manipulate postnatal RGCs in vivo is a powerful tool for developmental studies. (jove.com)

However, MSCs need to be expanded in vitro in order to obtain sufficient cells for clinical trials since they are extremely rare in various tissues. (hindawi.com)
Through the generation of several unique and scalable exosome producer cell lines, our CustomEX™ platform can be optimised for specific tissues targets and payloads leading to improvements in therapeutic outcome and a reduction in off-target effects. (euroland.com)
Although different stem cells, morphogens and scaffolds can be combined to simulate natural odontogenic regeneration, it is crucial to determine which options most closely replicate the characteristics of human dental tissues. (scielo.br)
The development of novel therapy against target critical and specific to the cancer cells but not to the normal tissues is expected to be efficacious and minimize damage to healthy cells. (inknowvation.com)
Although the number of transplantations each year has grown rapidly over the past two decades, the demand for transplantation using human cells, tissues and organs has also increased significantly, resulting in a continuing shortage of human material, particularly organs. (who.int)
As few countries are near to being self- sufficient in the provision of cells, tissues and organs for transplantation, new ways have been sought to increase the donation of human material. (who.int)
Likewise, commercial trade in cells, tissues and organs - and even trafficking involving human beings who are kidnapped or lured into other countries where they are forced to be "donors" - continues to be a serious problem, particularly in countries with substantial transplant tourism. (who.int)

In these experiments, we demonstrated that VZV interferes with NF-kappaB activation in cultured fibroblasts and in differentiated epidermal cells in skin xenografts of SCIDhu mice infected in vivo. (simulations-plus.com)
Human CMV grows only in human cells and replicates best in human fibroblasts. (medscape.com)

Therefore, the differential ability of dendritic cells and macrophages (splenic adherent cells [SACs]) to stimulate pancreatic islet allograft rejection in reversed alloxan-induced diabetic rats was examined. (diabetesjournals.org)

and stem cell regeneration. (lu.se)

Despite lower co-stimulatory molecule expression, ProGP-4-generated Class II+/CD11c+ cells stimulated proliferation of allogeneic T cells and an antigen-specific T cell hybridoma as efficiently as bone marrow derived dendritic cells from untreated mice. (medscimonit.com)
Cells for HSCT may be obtained from the patient himself or herself (autologous transplant) or from another person, such as a sibling or unrelated donor (allogeneic transplant) or an identical twin (syngeneic transplant). (medscape.com)

Cellular senescence, which is morphologically characterized by an enlarged and flattened cell shape, was first described by Hayflick [ 9 ]. (hindawi.com)
Cellular senescence refers to active cells that eventually enter a state of irreversible growth arrest. (hindawi.com)
These findings show that the vast majority of HIV-1 proviruses within expanded T cell clones, including intact proviruses, may be transcriptionally silent at any given time, implying that infected T cells may be able to be activated to proliferate without inducing the expression of the integrated provirus or, alternatelively, may be able to proliferate without cellular activation. (frontiersin.org)
Together, this approach would provide a non-conventional understanding of cell migration by correlating structural, morphological and dynamic cellular properties with the ability of cells to effectively migrate towards their target. (europa.eu)
ReNeuron has developed a proprietary stem cell-derived, exosome-based, drug delivery platform with customisable cellular targeting capabilities for the delivery of complex drug modalities. (euroland.com)
The capability of cell-penetrating peptides (CPPs) to transport cargos over different cellular membranes both in vitro and in vivo have drawn major attention in the past decade. (avhandlingar.se)
The translocation of CPPs into plant protoplasts might open up a new method for transformation of plant cells.Next, the cell-penetrating ability of the novel peptide YTA2 was characterized and it was established that chemical coupling between YTA2 and the protein cargo is not needed for the transport of the cargo over the cellular membrane in vitro. (avhandlingar.se)
As coculture gene expression better correlated with in vivo gene expression, we suggest that cellular cocultures may offer enhanced in vitro models for nanoparticle risk assessment and the reduction of in vivo toxicological testing. (cdc.gov)

Scholars@Duke publication: Targeting Dormant Ovarian Cancer Cells In Vitro and in an In Vivo Mouse Model of Platinum Resistance. (duke.edu)
The protein kinase C inhibitor UCN-01 (7-hydroxystaurosporine) has shown higher efficacy against slow proliferating and/or quiescent ovarian cancer cells. (duke.edu)
In this study, tumorigenic potential was assessed using anchorage-independent growth assays and spheroid-forming capacity, which was determined with ovarian cancer cell lines as well as primary ovarian cancers. (duke.edu)

In this in vivo examination, porous cylindrical hydroxyapatite scaffolds with a hollow center were immersed in 100 m g/ml laminin solution and air-dried. (scirp.org)
Rat bone marrow cells in 200 m l culture medium at 1 × 10 6 cells/ml were seeded in the scaffolds. (scirp.org)
It is concluded that laminin is useful for bone formation, as in an in vitro culture study using bone marrow cells, in hydroxyapatite scaffolds in vivo . (scirp.org)

Surprisingly, this bacterial resistance was evident 1 day after infection, and bacteria were cleared well before γδ T cell expansion was detected 6 days after infection. (jci.org)

Confirmation in vitro and in vivo that exosome targeting is dependent on cell source and selection of a specific exosome population results in the improved delivery of therapeutic payloads when compared to a conventional HEK293 exosome approach. (euroland.com)
Confirmation that therapeutic payloads can be successfully delivered in vivo using the CustomEX™ platform following systemic administration. (euroland.com)
This highly adaptable system provides a unique platform for live-cell ex vivo provocative testing of human tumor responsiveness to a range of physiological agonists or candidate therapeutic compounds. (duke.edu)

The present application proposes feasibility studies to investigate if this agent can be inhibit lung cancer, particularly on small cell lung carcinoma. (inknowvation.com)
Previous studies indicated that as few as 10 3 dendritic cells initiate allograft rejection in nondiabetic recipients. (diabetesjournals.org)
In vivo studies of microglial activation have been delayed by technical limitations to tracking microglial behavior without altering the CNS environment. (eugs.org)
Multi-walled carbon nanotube -induced gene expression in vitro: concordance with in vivo studies. (cdc.gov)

Rat bone marrow cells were obtained from femora of 6-week-old male Fischer 344 rats. (scirp.org)
For i n vitro examinations, 1 × 10 5 rat bone marrow cells in 2 ml culture medium were then cultured with the addition of dexamethasone and laminin. (scirp.org)
Rat bone marrow cells were also cultured in laminin-coated culture plates. (scirp.org)

Activation of muscle-specific genes in pigment, nerve, fat, liver, and fibroblast cell lines by forced expression of MyoD. (springer.com)
Microglia, which are CNS-resident neuroimmune cells, transform their morphology and size in response to CNS damage, switching to an activated state with distinct functions and gene expression profiles. (eugs.org)
This study compared global multi-walled carbon nanotube (MWCNT)-induced gene expression from human lung epithelial and microvascular endothelial cells in monoculture and coculture with gene expression from mouse lungs exposed to MWCNT. (cdc.gov)
Using a cutoff of 10% false discovery rate and 1.5 fold change, we determined that there were more concordant genes (gene expression both up- or downregulated in vivo and in vitro) expressed in both cell types in coculture than in monoculture. (cdc.gov)

The typical cell deaths are apoptosis, necrosis and autophagic cell death. (tmd.ac.jp)
In addition, we found that a cell cycle regulator, Plk1, switches the balance between TEAD/YAP-dependent necrosis and p73/YAP-dependent apoptosis by shifting the interaction partner of YAP from TEAD to p73 through YAP phosphorylation at Thr77. (tmd.ac.jp)

Characterization of dendritic cells generated in vivo by an E. coli derived chimeric dual receptor agonist. (medscimonit.com)
Dendritic cells and macrophages have been attributed with stimulatory capacity for in vivo and in vitro immune responses. (diabetesjournals.org)
Rats bearing established allografts were challenged with various dosages of donor-strain dendritic cells or SACs, and graft rejection was assessed by analysis of plasma glucose levels and/or histological criteria. (diabetesjournals.org)
Challenge stimuli consisting of ≤ 10 5 SACs or ≤10 4 dendritic cells failed to induce graft rejection. (diabetesjournals.org)
These findings indicate that dendritic cells are potent stimulator cells for in vivo immune responses. (diabetesjournals.org)

Intravenous treatment of infected, reconstituted hu-SCID mice with pamidronate, a human Vγ2Vδ2 T cell-specific aminobisphosphonate antigen, markedly increased the in vivo antibacterial effect of Vγ2Vδ2 T cells. (jci.org)
Memory effectors: a potent, IL-4-secreting helper T cell population that develops in vivo after restimulation with antigen. (umassmed.edu)

Pluripotent teratocarcinoma-thymus somatic cell hybrids. (springer.com)

Derubeis, A.R. and Cancedda, R. (2004) Bone marrow stromal cells (BMSCs) in bone engineering: Limitations and recent advances. (scirp.org)

Immunofluorescence staining confirmed human origin, identified differentiated epithelial cell types and verified the presence of supporting mesenchyme. (ca.gov)
However, the potential pathomechanisms of alveolar epithelial cell senescence and the impact of senolytic drugs on senescent lung cells and fibrosis remain unknown. (ersjournals.com)

Uptake of chemically modified low density lipoproteins in vivo is mediated by specific endothelial cells. (rupress.org)
Because macrophages, Kupffer cells, and to a lesser extent, endothelial cells metabolize these modified lipoproteins in vitro, it was of interest to determine whether endothelial cells or macrophages could be responsible for the in vivo uptake of these lipoproteins. (rupress.org)
Isolated sinusoidal endothelial cells from the rat liver displayed saturable, high affinity binding of AcAc LDL (Kd = 2.5 X 10(-9) M at 4 degrees C), and were shown to degrade AcAc LDL 10 times more effectively than aortic endothelial cells. (rupress.org)
These data indicate that specific sinusoidal endothelial cells, not the macrophages of the reticuloendothelial system, are primarily responsible for the removal of these modified lipoproteins from the circulation in vivo. (rupress.org)

Dental pulp stem cells (DPSC) seem to be the most appropriate cell group for the regenerative approach. (scielo.br)
Dental pulp stem cells: state of the art and suggestions for a true translation of research into therapy. (scielo.br)
Odontogenic capability: bone marrow stromal stem cells versus dental pulp stem cells. (scielo.br)

However, the immune system relies on a complex network of cell-to-cell interactions which remains largely unexplored and difficult to be interpreted. (usi.ch)
Together, iMC expand independent of the type of anti-tumor response and are not immune-suppressive in a cell-autonomous fashion. (mdc-berlin.de)

In this paper, we review the different protocols available to study human Plasmodium species either by using stem cell or alternative animal models. (wjgnet.com)
Thanks to recent progresses, stem cells have been extensively employed to study Plasmodium liver and blood cycle in vitro . (wjgnet.com)
The unique advantages of highly varied composition, multicompartmentalized architecture, and preserved functionality in vivo open new biological avenues ranging from the study of biorelevant processes in robust cell-like environments to the production of specific bioactive compounds. (nih.gov)
The purpose of this study is to evaluate markers in the eye that can predict and help diagnose limbal stem cell deficiency. (ca.gov)
The fact that molecules facilitating the motility of zebrafish PGCs are evolutionary conserved and the finding that the cells are directed by chemokines, molecules that control a wide range of cell trafficking events in vertebrates, make this in vivo study of particular importance. (europa.eu)
This study compares in vitro and in vivo models of lipid digestion from almond particles within a complex food matrix (muffins) investigating whether the cell-wall barrier regulates the bioaccessibility of nutrients within this matrix. (nuthealth.org)

The developmental capacity of nuclei transplanted from keratinized skin cells of adult frogs. (springer.com)
Viable offspring derived from fetal and adult mammalian cells. (springer.com)
Unlike embryonic stem cells, adult stem cells (MSCs) have a limited lifespan and stop proliferating during in vitro culture due to replicative senescence [ 8 ]. (hindawi.com)

J Cell Biol (1985) 100 (1): 103-117. (rupress.org)

Figure 4: Quantitative analysis of drug response to paclitaxel treatment at a single-cell level. (nature.com)

Plotting the proportion of maximally responsive tumor cells as a function of calcium concentration yields a sigmoid dose-response curve with a calculated calcium EC50 value significantly elevated above published reference values for wild-type calcium-sensing receptor (CASR) sensitivity. (duke.edu)

Intellia believes the CRISPR/Cas9 technology has the potential to transform medicine by both producing therapeutics that permanently edit and/or correct disease-associated genes in the human body with a single treatment course, and creating enhanced engineered cells that can treat oncological and immunological diseases. (newswise.com)
When reduced to only those genes involved in inflammation and fibrosis, known outcomes of in vivo MWCNT exposure, there were more disease-related concordant genes expressed in coculture than monoculture. (cdc.gov)

These cells make up 2-5% percent of human peripheral blood T cells but expand to make up 8-60% of peripheral blood T cells during bacterial and parasitic infections. (jci.org)
Peripheral myelination is a dynamic process orchestrated by axons and Schwann cells. (jneurosci.org)
ProGP-4 possesses cross-species activity and treatment of mice with ProGP-4 results in increases in the number of WBC and Class II+/CD11c+ cells in both spleen and peripheral blood. (medscimonit.com)
RESULTS: Administration of this dual receptor agonist to mice resulted in dose-dependent increases in the numbers of total white blood cells and Class II+/CD11c+ cells in spleen and peripheral blood. (medscimonit.com)
The National Marrow Donor Program (NMDP), founded in 1986, and the World Marrow Donor Association (WMDA), founded in 1988, were established to (1) locate and secure appropriate unrelated-donor HSCT sources for patients by promoting volunteer donation of bone marrow and peripheral blood stem cells in the community and (2) promote ethical practices of sharing stem cell sources by need, rather than by geographic location of the donor. (medscape.com)

The developmental capacity of nuclei taken from differentiating endoderm cells of Xenopus laevis. (springer.com)
The developmental capacity of nuclei taken from intestinal epithelium cells of feeding tadpoles. (springer.com)
Transplantation of living nuclei from blastula cells into enucleated frogs' eggs. (springer.com)
Figure 2: Automatic segmentation of cell nuclei. (nature.com)

The implants achieve rapid and efficient repair of a calvarial defect with a very low incidence of teratomas (they are seen in 2 of 42 mice) after injection of 1 million human pluripotent cells. (nature.com)
Embryonic stem cell lines derived from human blastocysts. (springer.com)
Evans MD, Kelley J. US attitudes toward human embryonic stem cell research. (springer.com)
Depletion of Vδ2 T cells exacerbated bacterial infection ( a - c ), whereas live bacterial product augmented the antibacterial effects of Vγ2Vδ2 T cells ( d - g ). ( a ) All SCID-beige mice infected with a lethal dose of E. coli (1 × 10 7 CFUs, administered intraperitoneally) were dead within 2 days, whereas all mice reconstituted with human PBMCs survived this lethal infection. (jci.org)
In addition to the new in vitro systems, in recent years there were also significant advances in the development of new animal models that allows studying the entire cell cycle of human malaria. (wjgnet.com)
In previous work, human fetal intestinal cells developed epithelium without mesenchyme following implantation in mice. (ca.gov)
Immunofluorescence staining demonstrated human origin and the three differentiated cell types of mature colon epithelium. (ca.gov)
Using this method, combined with proteomic profiling, we show that PC3 human prostate cancer organoids secrete EVs with previously undefined protein cargo, which substantially differs from EV cargo of 2D cultured cells. (biorxiv.org)
editors Culture of human stem cells. (scielo.br)
Myelodysplastic Syndromes Are Propagated by Rare and Distinct Human Cancer Stem Cells In Vivo. (lu.se)
Evidence for distinct human cancer stem cells (CSCs) remains contentious and the degree to which different cancer cells contribute to propagating malignancies in patients remains unexplored. (lu.se)
These findings provide definitive evidence for rare human MDS-SCs in vivo, with extensive implications for the targeting of the cells required and sufficient for MDS-propagation. (lu.se)
First, we are utilizing a modified chromatin immunoprecipitation approach to capture and identify genomic DNA target sequences conditionally associated with pRB-containing complexes recovered from intact chromatin in untransformed primary human cells. (duke.edu)

All identified somatically acquired genetic lesions were backtracked to distinct MDS-SCs, establishing their distinct MDS-propagating function in vivo. (lu.se)

Stem cells and regenerative medicine - future perspectives. (springer.com)
Stem cells in regenerative medicine: introduction. (springer.com)

Using a mouse model, we show that nuclear factor κB activation in Schwann cells is not required for myelination in vivo . (jneurosci.org)

Here, these limitations are overcome and customizable cell mimic is achieved-molecular factories (MFs)-by supplementing giant plasma membrane vesicles derived from donor cells with nanometer-sized artificial organelles (AOs). (nih.gov)

doi:10.1016/j.cell.2009.11.021. (wikipedia.org)

Anatomy50

Organisms24

Diseases11

Chemicals and Drugs52

Analytical, Diagnostic and Therapeutic Techniques and Equipment74

Phenomena and Processes49

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture2

Information Science6

Health Care5

Hematopoietic stem2

Mesenchymal10

Tumors4

Vitro studies2

Differentiation9

Extracellular7

Retinal2

Biology2

Tissue24

Xenograft1

Progenitor Cells1

Microscopy3

Functional7

Methods4

Postnatal1

Tissues7

Fibroblasts2

Pancreatic1

Regeneration1

Allogeneic2

Cellular8

Ovarian3

Scaffolds3

Expansion1

Therapeutic3

Studies4

Bone Marro3

Expression4

Apoptosis2

Dendritic cells5

Antigen2

Somatic cell1

Stromal cells1

Epithelial cell2

Endothelial cells4

Dental pulp stem cells3

Immune2

Study6

Adult3

19851

Paclitaxel1

Tumor cells1

Genes2

Peripheral5

Nuclei4

Human13

Distinct1

Regenerative medicine2

Nuclear1

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